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https://www.readbyqxmd.com/read/28092911/behaviour-of-cd11b-positive-cells-in-an-animal-model-of-laser-induced-choroidal-neovascularisation
#1
Lu Li, Peter Heiduschka, Anne F Alex, Daniel Niekämper, Nicole Eter
BACKGROUND/AIM: Immune cells, e.g. microglial cells of the retina, appear to be involved in pathological processes in neovascular age-related macular degeneration. Therefore, the purpose of this study was to immunohistochemically check the expression of various factors and cytokines by CD11b-positive (CD11b+) immune cells in an animal model of choroidal neovascularisation (CNV). METHODS: We used the animal model of laser-induced CNV in mice. Eyes were isolated at 1, 4, 7, and 14 days after laser treatment...
January 17, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/28092864/augmentation-of-humoral-and-cellular-immunity-in-response-to-tetanus-and-diphtheria-toxoids-by-supercritical-carbon-dioxide-extracts-of-hippophae-rhamnoides-l-leaves
#2
Bindhya Jayashankar, Divya Singh, Himanshi Tanwar, K P Mishra, Swetha Murthy, Sudipta Chanda, Jigni Mishra, R Tulswani, K Misra, S B Singh, Lilly Ganju
Hippophae rhamnoides L. commonly known as Seabuckthorn (SBT), a wild shrub of family Elaegnacea, has extensively used for treating various ailments like skin diseases, jaundice, asthma, lung troubles. SBT leaves have been reported to possess several pharmacological properties including immunomodulatory, antioxidant, anti-inflammatory, antimicrobial and tissue regeneration etc. The present study was undertaken to evaluate the adjuvant property of supercritical carbon dioxide extracts (SCEs 300ET and 350ET) of SBT leaves in balb/c mice immunized with Tetanus and Diphtheria toxoids...
January 13, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28092841/phytochemical-study-and-protective-effect-of-trigonella-foenum-graecum-fenugreek-seeds-against-carbon-tetrachloride-induced-toxicity-in-liver-and-kidney-of-male-rat
#3
Sakhria Mbarki, Hichem Alimi, Hafsia Bouzenna, Abdelfettah Elfeki, Najla Hfaiedh
Liver and kidney diseases are a global concern, therefore considerable efforts to obtain fine herbs useful as drugs from medicinal plants are currently in progress. The aim of this work was to study the antioxidant effects of previous supplementation with fenugreek seeds (FS) against carbon tetrachloride (CCl4) toxicity in the liver and kidney. CCl4 toxicity was induced by one dose (i.g. 5ml CCl4/kg of body weight, 50% CCl4 in olive oil) after 7 weeks of normal diet or diet rich in 10% of grinded fenugreek seeds (20g of pellet rat food/rat/day)...
January 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28092811/towards-the-structure-of-the-tir-domain-signalosome
#4
REVIEW
Surekha Nimma, Thomas Ve, Simon J Williams, Bostjan Kobe
TIR (Toll/interleukin-1 receptor/resistance protein) domains feature in animal, plant and bacterial proteins involved in innate immunity pathways and associated processes. They function through protein:protein interactions, in particular self-association and homotypic association with other TIR domains. Structures of TIR domains from all phyla have been determined, but common association modes have only emerged for plant and bacterial TIR domains, and not for mammalian TIR domains. Numerous attempts involving hybrid approaches, which have combined structural, computational, mutagenesis and biophysical data, have failed to converge onto common models of how these domains associate and function...
January 13, 2017: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/28092770/magnolin-inhibits-prostate-cancer-cell-growth-in-vitro-and-in-vivo
#5
Yeqing Huang, Xiangyu Zou, Xiaowen Zhang, Feng Wang, Weidong Zhu, Guangyuan Zhang, Jun Xiao, Ming Chen
BACKGROUND: Magnolin is the most active ingredient in the herb Magnolia fargesii, which has been traditionally used in oriental medicine to treat headaches and nasal congestion. Recent researches demonstrate that Magnolin inhibits cancer cell migration and invasion. MATERIALS AND METHODS: This study used cell culture and the BALB/c nu/nu mouse xenograft model to investigate whether or not magnolin can inhibit the growth of PC3 and Du145 prostate cancer cells. MTT assay and flow cytometry were performed to estimate the proliferation, cycle, and apoptosis of the cells in vitro...
January 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28092763/growth-and-antioxidant-defense-responses-of-wheat-seedlings-to-di-n-butyl-phthalate-and-di-2-ethylhexyl-phthalate-stress
#6
Minling Gao, Youming Dong, Ze Zhang, Wenhua Song, Yun Qi
Phthalate acid esters (PAEs) are vital environmental hormone-like chemicals that are noxious to plants, animals, and human beings. In this study, the influences of di-n-butyl phthalate (DBP) and di (2-ethylhexyl) phthalate (DEHP) on the seed germination, root morphology, and various physiological changes of wheat seedlings were investigated by analyzing superoxide anion (O2(-)) accumulation, antioxidant enzyme activity, and lipid peroxidation. DBP and DEHP were found to obviously inhibit germination only at high concentrations, but significantly affected root morphology even at lower concentrations...
January 5, 2017: Chemosphere
https://www.readbyqxmd.com/read/28092742/short-term-in-vivo-exposure-to-graphene-oxide-can-cause-damage-to-the-gut-and-testis
#7
Marta Dziewięcka, Julia Karpeta-Kaczmarek, Maria Augustyniak, Magdalena Rost-Roszkowska
Graphene oxide (GO) has unique physicochemical properties and also has a potentially widespread use in every field of daily life (industry, science, medicine). Demand for nanotechnology is growing every year, and therefore many aspects of its toxicity and biocompatibility still require further clarification. This research assesses the in vivo toxicity of pure and manganese ion-contaminated GO that were administrated to Acheta domesticus with food (at 200mgkg(-1) of food) throughout their ten-day adult life...
January 9, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28092740/temporal-variations-in-early-developmental-decisions-an-engine-of-forebrain-evolution
#8
REVIEW
H Bielen, S Pal, S Tole, C Houart
Tight control of developmental timing is pivotal to many major processes in developmental biology, such as patterning, fate specification, cell cycle dynamics, cell migration and connectivity. Temporal change in these ontogenetic sequences is known as heterochrony, a major force in the evolution of body plans and organogenesis. In the last 5 years, studies in fish and rodents indicate that heterochrony in signaling during early development generates diversity in forebrain size and complexity. Here, we summarize these findings and propose that, additionally to spatio-temporal tuning of neurogenesis, temporal and quantitative modulation of signaling events drive pivotal changes in shape, size and complexity of the forebrain across evolution, participating to the generation of diversity in animal behavior and emergence of cognition...
January 13, 2017: Current Opinion in Neurobiology
https://www.readbyqxmd.com/read/28092737/complex-molecular-changes-induced-by-chronic-progestogens-exposure-in-roach-rutilus-rutilus
#9
Gabor Maasz, Zita Zrinyi, Peter Takacs, Sandor Lovas, Istvan Fodor, Tibor Kiss, Zsolt Pirger
In our previous study, we measured 0.23-13.67ng/L progestogens (progesterone, drospirenone, levonorgestrel) in natural waters in the catchment area of the largest shallow lake of Central Europe, Lake Balaton. Progestogen contaminations act as potent steroids with mixed progestagenic, androgenic and mild estrogenic effects that is why our aim was to investigate the morphological and molecular effects of mixture of progesterone, drospirenone, and levonorgestrel in environmentally relevant (10ng/L) and higher (50 and 500ng/L) exposure concentrations in common roach, Rutilus rutilus...
January 13, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28092693/pathogenic-streptococcus-strains-employ-novel-escape-strategy-to-inhibit-bacteriostatic-effect-mediated-by-mammalian-peptidoglycan-recognition-protein
#10
Jing Wang, Youjun Feng, Changjun Wang, Swaminath Srinivas, Chen Chen, Hui Liao, Elaine He, Shibo Jiang, Jiaqi Tang
Pathogenic streptococcal species are responsible for some of the most lethal and prevalent animal and human infections. Previous reports have identified a candidate pathogenicity island (PAI) in two highly virulent clinical isolates of Streptococcus suis type 2 (SS2), a causative agent of high-mortality streptococcal toxic shock syndrome (STSS). This PAI contains a Type-IVC secretion system C subgroup (Type-IVC secretion system) that is involved in the secretion of unknown pathogenic effectors that are responsible for STSS caused by highly virulent strains of SS2...
January 16, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28092624/changes-in-reticulorumen-content-temperature-and-ph-according-to-time-of-day-and-yearly-seasons
#11
R Antanaitis, V Žilaitis, V Juozaitienė, R Stoškus, M Televičius
The monitoring of rumen content temperature can be useful for the evaluation of cow health condition and heat. The aim of this research was to evaluate the influence of the circadian rhythm (time of day) and season on reticulorumen acidity (pH) and temperatures (RT) in lactating dairy cows. The research was performed on ten 2nd - lactation, clinically healthy Lithuanian Black and White fresh dairy cows (up to 1 day after calving). The cows were milked twice daily at 05:00 and 17:00. The cows were kept in a loose housing system, and were fed a feed ration throughout the year at the same time, balanced according to their physiological needs...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28092623/ultrasonography-a-method-used-for-pregnancy-imaging-of-the-fire-salamander-salamandra-salamandra
#12
A Najbar, Z Kiełbowicz, J Szymczak, M Ogielska
Ultrasound imaging has more frequently been used in veterinary medicine of amphibians and reptiles. In this study, we have verified the usefulness of ultrasound imaging in pregnancy determination of the fire salamander Salamandra salamandra. We have also undertaken to estimate the number of larvae and their developmental stage directly in the oviducts. Three gravid females from Lower Silesia (southern Poland) were examined. Due to the small size of the scanned animals, and the particular arrangement of embryos in the oviducts and ultrasound beams dispersal, the method proved to be inaccurate...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28092617/supplementation-of-pigs-diet-with-zinc-and-copper-as-alternative-to-conventional-antimicrobials
#13
B Dębski
Modern commercial pig farming systems inflict increased stress in animals, which often leads to various negative changes in the gastro-intestinal tract, especially in the case of piglets. Ban of antibiotics, used as growth promoters, has caused a need for alternatives to conventional antimicrobials in swine diets. Use of pre-/or probiotics, organic acids and plant extracts is often recommended, but it seems that zinc oxide and cooper salts, which were traditionally included in high doses to piglets diet, possess the highest efficacy...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28092616/generation-and-characterization-of-a-monoclonal-antibody-against-duck-tembusu-virus-envelope-protein
#14
K Han, D Zhao, Y Liu, Q Liu, X Huang, J Yang, K Bi, T Xu, Y Li
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28092612/evaluation-of-serum-cytokine-levels-in-recurrent-airway-obstruction
#15
A Niedźwiedź, H Borowicz, K Kubiak, J Nicpoń, P Skrzypczak, Z Jaworski, M Cegielski, J Nicpoń
Recurrent airway obstruction (RAO) represents a serious health problem and is traditionally classified as an allergic disease, where contact with an antigen can induce clinical airway inflammation, bronchial hyper-responsiveness and reversible airway obstruction. Previous studies have demonstrated the presence of the Th2 response in the lungs of human patients with asthma and horses with heaves. These cells are involved in the production of cytokines which regulate the synthesis of immunoglobulins. 40 horses were evaluated: 30 horses with RAO and 10 healthy animals...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28092610/effects-of-antioxidant-vitamins-a-d-e-and-trace-elements-cu-mn-se-zn-on-some-metabolic-and-reproductive-profiles-in-dairy-cows-during-transition-period
#16
A Omur, A Kirbas, E Aksu, F Kandemir, E Dorman, O Kaynar, O Ucar
The objective of this study was to determine the effects of some antioxidant vitamins and trace elements on some metabolic and postpartum reproductive profiles in dairy cows during transition period. In the study, altogether 20 clinically healthy Brown Swiss dairy cows (aged 4-5 years-old) under the same management and feeding conditions in periparturient period were used. The animals were divided into two equal groups: control (C) and treatment (T) group (n=10 for each group). Vitamins (A, D, E) and trace elements (Cu, Mn, Se, Zn) were administered intramuscularly into the cows of the T group, while isotonic saline, as placebo, was injected subcutaneously into those in the C group...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28092607/proanp-as-a-screening-biomarker-for-hypertrophic-cardiomyopathy-in-maine-coon-cats
#17
M Parzeniecka-Jaworska, M Garncarz, W Kluciński
: The aim of this study was to determine if atrial natriuretic peptide can be used as an early screening tool for hypertrophic cardiomyopathy in Maine coon cats. ANIMALS: The study was performed in 43 Maine coon cats of both sexes, aged 11 to 92 months. Clinical and echocardiographic examinations were done and proANP serum concentrations were measured every three months over a period of one year (each cat had a total of five examinations). Cats were divided into 3 groups based on echocardiographic results: group 1 - healthy cats, group 2 - cats with unequivocal hypertrophic cardiomyopathy results, group 3 - cats with HCM...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28092603/gut-microbiota-isolated-from-the-european-pond-turtle-emys-orbicularis-and-its-antimicrobial-resistance
#18
M Ruzauskas, S Misyte, L Vaskeviciute, Z Mikniene, R Siugzdiniene, I Klimiene, A Pikuniene, J Kucinskiene
The aim of the study was to isolate cultivable gut microbiota from European pond turtles kept at the Lithuanian Zoo and to determine antimicrobial resistance of the isolates. The study subjects included 8 elderly turtles living at the Lithuanian Zoo for about 50 years as well as their offspring - 24 young individuals (1-2 years old) that were hatched at the same zoo. Animals were not exposed by treatment with antimicrobials during the last 3 years. Gut samples were taken from the cloaca and inoculated onto universal media...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28092600/influence-of-horse-breed-on-transepidermal-water-loss
#19
M P Szczepanik, P M Wilkołek, Ł R Adamek, M Pluta, M Gołyński, W Sitkowski, G Kalisz, I Taszkun, Z J H Pomorski
Non-invasive methods of skin condition assessment include, among others, the evaluation of transepidermal water loss (TEWL). The aim of the study was to examine whether TEWL values depend on horse breed. The study was conducted on four breeds: "Felin" ponies (FP) (n=16), Polish koniks (PK) (n=15), Polish cold-blooded horses (PcbH) (n=11) and Wielkopolska horses (WH) (n=12). It was found that horse breed influences TEWL values. In the neck region, statistically significant differences were found between PK and FP (p=0...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28092599/wild-boars-sus-scrofa-as-bioindicators-of-environmental-levels-of-selenium-in-poland
#20
E Nowakowska, B Pilarczyk, R Pilarczyk, A Tomza-Marciniak, M Bąkowska, A Marciniak
The objective of the study was to determine selenium content in selected organs (liver, kidney) of wild boars from different regions of Poland. Materials for the study were obtained from 28 sites located in 16 provinces of Poland. Selenium concentrations in organs were determined using spectrofluorometric methods after wet mineralization in HNO3 and HClO4 mixture. Mean selenium concentrations in the investigated wild boars from Poland were 0.230 μg/g wet weight in the liver and 1.327 μg/g w.w. in the kidneys...
December 1, 2016: Polish Journal of Veterinary Sciences
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