keyword
https://read.qxmd.com/read/38237593/a-gut-microbiota-bile-acid-axis-promotes-intestinal-homeostasis-upon-aspirin-mediated-damage
#1
JOURNAL ARTICLE
Ting Li, Ning Ding, Hanqing Guo, Rui Hua, Zehao Lin, Huohuan Tian, Yue Yu, Daiming Fan, Zuyi Yuan, Frank J Gonzalez, Yue Wu
Aspirin-related gastrointestinal damage is of growing concern. Aspirin use modulates the gut microbiota and associated metabolites, such as bile acids (BAs), but how this impacts intestinal homeostasis remains unclear. Herein, using clinical cohorts and aspirin-treated mice, we identified an intestinal microbe, Parabacteroides goldsteinii, whose growth is suppressed by aspirin. Mice supplemented with P. goldsteinii or its BA metabolite, 7-keto-lithocholic acid (7-keto-LCA), showed reduced aspirin-mediated damage of the intestinal niche and gut barrier, effects that were lost with a P...
January 11, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38173516/chemo-informatics-applications-in-the-design-of-novel-7-keto-sempervirol-derivatives-as-smcb1-inhibitors-with-potential-for-treatment-of-schistosomiasis
#2
JOURNAL ARTICLE
Salim Bitrus Anyubaga, Gideon Adamu Shallangwa, Adamu Uzairu, Stephen Eyije Abechi
The quest for a sound treatment on the vulnerable population suffering and dying as a result of the blood flukes, S. mansoni is on the increase because both Praziquantel and Oxamniquine widely used for the treatment of Schistosomiasis for over 51 years suffer resistance and recurrence. Here-in, chemo-informatics techniques such as QSAR modeling, pharmacokinetic, docking alongside MD simulation were harnessed in designing novel 7-keto- sempevirolsempevirol derivatives that are more competent against S. mansoni...
January 15, 2024: Heliyon
https://read.qxmd.com/read/37512573/variation-in-terpenoid-and-flavonoid-content-in-different-samples-of-salvia-semiatrata-collected-from-oaxaca-mexico-and-its-effects-on-antinociceptive-activity
#3
JOURNAL ARTICLE
Nancy Ortiz-Mendoza, Rubén San Miguel-Chávez, Martha Juana Martínez-Gordillo, Francisco Alberto Basurto-Peña, Mariana Palma-Tenango, Eva Aguirre-Hernández
Salvia semiatrata Zucc. (Lamiaceae) is endemic to Oaxaca, Mexico, and is known for its analgesic properties. Terpenoids and phenolic compounds with antinociceptive potential have been characterised from this species. The aim of this research was to determine the variation in terpenoids and flavonoids in ethyl acetate extracts of S. semiatrata collected from ten different localities, as well as to evaluate the antinociceptive effect between plants with higher and lower contents of these secondary metabolites...
July 20, 2023: Metabolites
https://read.qxmd.com/read/36566478/a-systematic-review-of-the-impact-of-7-keto-dhea-on-body-weight
#4
REVIEW
Nishanthini Jeyaprakash, Sara Maeder, Heidrun Janka, Petra Stute
7-Keto-DHEA has been commercially advertised as a dietary supplement to support weight loss. The objective of the present systematic review it to summarize the evidence supporting the use of 7-keto-DHEA in overweight and obese population. The systematic search was conducted in Medline, Embase, Cochrane Library, CINAHL, Web of Science, Scopus, ICTRP, and ClinicalTrials.gov. Additionally, reference lists of eligible studies were considered, and authors of relevant studies were contacted. Two authors independently screened the studies against the inclusion criteria and assessed their risk of bias...
September 2023: Archives of Gynecology and Obstetrics
https://read.qxmd.com/read/36450588/omphalines-a-e-ent-rosane-type-diterpenoids-from-the-madagascar-endemic-plant-omphalea-oppositifolia
#5
JOURNAL ARTICLE
Susumu Kawakami, Chieko Kanagawa, Liva Harinantenaina Rakotondraibe, Masanori Inagaki, Motohiro Nishimura, Hideaki Otsuka, Toshio Seyama, Katsuyoshi Matsunami, Falitiana Marrino Rakotoarisoa, Stéphan Richard Rakotonandrasana, Alain Michel Ratsimbason
From the less polar fraction of the MeOH extract of the leaves and twigs of Omphalea oppositifolia, five new ent-rosane-type diterpenoids, named omphalines A-E (1-5), were isolated together with one known compound, 7-keto-ent-kaurane-16β,17-diol (6), by a combination of various kinds of chromatography. The structure of omphaline A (1) was elucidated to be 19-nor-ent-rosane-4,15-diene-2β,6α-diol-3-one. Omphalines B (2), C (3), D (4), and E (5) possessed two double bonds at 5- and 15-positions, and hydroxy functional groups at 3β-, 2α,3α-, 2α,3β-, and 2α,19-positions, respectively...
2022: Chemical & Pharmaceutical Bulletin
https://read.qxmd.com/read/36431934/chemical-indices-and-kinetic-evaluation-of-%C3%AE-sitosteryl-oleate-oxidation-in-a-model-system-of-bulk-oil
#6
JOURNAL ARTICLE
Manat Chaijan, Worawan Panpipat, Ling-Zhi Cheong
Concerns have been raised about the safety and tolerability of phytosterol esters due to their vulnerability to oxidation. Herein, oxidation of the unsaturated fatty acid-phytosterol ester, namely β-sitosteryl oleate, was observed in comparison to native β-sitosterol after accelerated storage at 65 °C for 35 days in a bulk oil model system. Depending on the sterol structure, various chemical indices of lipid oxidation, including hydroperoxide value (HPV), thiobarbituric acid reactive substances (TBARS), p -anisidine value (AnV), and 7-keto derivatives, changed at varying rates in both samples...
November 14, 2022: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/36361795/neuropeptide-y-promotes-human-m2-macrophage-polarization-and-enhances-p62-sqstm1-dependent-autophagy-and-nrf2-activation
#7
JOURNAL ARTICLE
Elisabetta Profumo, Elisa Maggi, Marzia Arese, Claudio Di Cristofano, Bruno Salvati, Luciano Saso, Rita Businaro, Brigitta Buttari
Neuropeptide Y (NPY) is an abundantly expressed peptide capable of modulating innate and adaptive immune responses and regulating chemotaxis and cytokine secretion by macrophages. Abnormal regulation of NPY is involved in the development of atherosclerosis. The inflammatory infiltrate within atherosclerotic plaque is characterized by accumulation of macrophages, which are subject to reprogram their phenotypes in response to environmental signals. Macrophage number and phenotype influence plaque fate. Here, we investigated the effect of NPY on the changes in phenotype and functions of human macrophages, from the pro-inflammatory phenotype M1 to the reparative M2, indicative of atherosclerosis regression or stabilization...
October 27, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/36195466/gc-ms-analysis-of-oxysterols-and-their-formation-in-cultivated-liver-cells-hepg2
#8
JOURNAL ARTICLE
Elisabeth Koch, Mustafa Bagci, Michael Kuhn, Nicole M Hartung, Malwina Mainka, Katharina M Rund, Nils Helge Schebb
Oxysterols play a key role in many (patho)physiological processes and they are potential biomarkers for oxidative stress in several diseases. Here we developed a rapid gas chromatographic-mass spectrometry-based method for the separation and quantification of 11 biologically relevant oxysterols bearing hydroxy, epoxy, and dihydroxy groups. Efficient chromatographic separation (resolution ≥ 1.9) was achieved using a medium polarity 35%-diphenyl/65%-dimethyl polysiloxane stationary phase material (30 m × 0...
October 4, 2022: Lipids
https://read.qxmd.com/read/35872051/regulation-of-gut-microbiota-of-astragali-radix-in-treating-for-chronic-atrophic-gastritis-rats-based-on-metabolomics-coupled-with-16s-rrna-gene-sequencing
#9
JOURNAL ARTICLE
Yuetao Liu, Congcong Cai, Xuemei Qin
Astragali Radix (HQ), a common traditional Chinese medicine (TCM), is widely used to treat chronic atrophic gastritis (CAG). However, its mechanism in treating CAG is still not clear. Accumulating evidence highlights the link between gut microbiota and CAG. We hypothesized that the gut microbiota might be involved in the effect of HQ. Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-Q-TOF/MS) based metabolomics and 16S rRNA gene sequencing techniques of the cecal contents were applied to study its mechanisms...
September 25, 2022: Chemico-biological Interactions
https://read.qxmd.com/read/35840217/impact-of-photosensitizers-and-light-wavelength-on-photooxidation-of-phytosterols-in-soymilk-emulsions
#10
JOURNAL ARTICLE
Bo-Wen Yang, Tao Xu, Yan Liu, Tian Zhao, Fan Xiao, Bai-Yi Lu
The impact of photosensitizer and wavelength on photooxidation of phytosterols (PS) in soymilk and the oxidative stability of lipid and protein was determined. The oxidation of lipid and the consumption of dissolved oxygen showed a close relationship with PS oxidation. Riboflavin (Rb) leads to extra oxidation of both lipid and protein, and chlorophyll (Chl) prefer to absorb to and change the structure of protein. The influence of Rb and Chl on PS degradation under different wavelengths of light in the range of 365-665 nm was measured...
August 2022: Food Research International
https://read.qxmd.com/read/35063161/determination-of-phytosterol-oxidation-products-in-pharmaceutical-liposomal-formulations-and-plant-vegetable-oil-extracts-using-novel-fast-liquid-chromatography-tandem-mass-spectrometric-methods
#11
JOURNAL ARTICLE
Asmita Poudel, George Gachumi, Randy Purves, Ildiko Badea, Anas El-Aneed
Phytosterol oxidation products (POPs) formed by the auto-oxidation of phytosterols can lead to negative health consequences. New liquid chromatography-tandem mass spectrometry (LC-MS/MS) quantitative and qualitative approaches were developed. For quantification, sixteen phytosterol oxidation products (POPs) in liposomal formulations; namely 7-keto, 7-hydroxy, 5,6-epoxy, and 5,6-dihydroxy derivatives of brassicasterol, campesterol, stigmasterol, and β-sitosterol were quantified. The method has a short run time of 5 min, achieved on a poroshell C18 column, using isocratic elution...
February 15, 2022: Analytica Chimica Acta
https://read.qxmd.com/read/34738273/metabolomics-workflow-as-a-driven-tool-for-rapid-detection-of-metabolites-in-doping-analysis-development-and-validation
#12
JOURNAL ARTICLE
Patrizia Leogrande, Daniel Jardines, Dayamin Martinez-Brito, Eleonora Domenici, Xavier de la Torre, Maria Kristina Parr, Francesco Botrè
RATIONALE: This work demonstrates the high potential of combining high-resolution mass spectrometry with chemometric tools, using metabolomics as a guided tool for anti-doping analysis. The administration of 7-keto-DHEA was studied as a proof-of-concept of the effectiveness of the combination of knowledge-based and machine-learning approaches to differentiate the changes due to the athletic activities from those due to the recourse to doping substances and methods. METHODS: Urine samples were collected from five healthy volunteers before and after an oral administration by identifying three time intervals...
January 30, 2022: Rapid Communications in Mass Spectrometry: RCM
https://read.qxmd.com/read/34685915/the-bark-of-picea-abies-l-a-waste-from-sawmill-as-a-source-of-valuable-compounds-phytochemical-investigations-and-isolation-of-a-novel-pimarane-and-a-stilbene-derivative
#13
JOURNAL ARTICLE
Stefania Sut, Valeria Baldan, Marta Faggian, Irene Ferrarese, Erica Maccari, Eduardo Teobaldo, Nicola De Zordi, Paolo Bertoni, Gregorio Peron, Stefano Dall'Acqua
In this work, the sawmill waste from Picea abies debarking was considered as source of valuable phytoconstituents. The extraction was performed using different ethanol/water mixtures, and characterization was obtained by LC-MSn . This latter revealed flavonoid glycosides, lignans, and procyanidins. Extraction with organic solvents (dichloromethane and methanol) and chromatographic separations of the obtained extracts by silica column followed by semi-preparative HPLC led to the isolation of polyphenols and terpenoids such as 21 α -metoxy-serrat-14-en-3-one, 21 α -hydroxy-serrat-14-en-3-one, pinoresinol, dehydroabietic acid, 15-hydroxy-dehydroabietic acid, 7-oxo-dehydroabietic acid, pimaric acid, 9 β -pimara-7,15-dien-19-ol, 13-epi-manoyl oxide, taxifolin-3'-O-glucopyranoside, trans-astringin, and piceasides...
October 4, 2021: Plants (Basel, Switzerland)
https://read.qxmd.com/read/34586790/sterols-and-sterol-oxidation-products-effect-of-dietary-intake-on-tissue-distribution-in-apoe-deficient-mice
#14
JOURNAL ARTICLE
Mengmeng Wang, Bowen Yang, Ping Shao, Fan Jie, Xuan Yang, Baiyi Lu
Sterols and sterol oxidation products (SOPs) are well-known dietary factors influencing atherosclerosis; however, their distribution in vivo after dietary sterol/SOP intake is still unclear. Here, we investigated the tissue distribution of sterols and SOPs in ApoE-/- mice after dietary exposure to diets supplemented with phytosterols (PS), phytosterol oxidation products (POPs), or cholesterol oxidation products (COPs). The results showed that PS intake reduced cholesterol in serum and the liver but increased cholesterol in the brain...
October 13, 2021: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/34397319/an-overview-of-7%C3%AE-and-7%C3%AE-hydroxysteroid-dehydrogenases-structure-specificity-and-practical-application
#15
JOURNAL ARTICLE
Deshuai Lou, Xi Liu, Jun Tan
7α-Hydroxysteroid dehydrogenase and 7β-hydroxysteroid dehydrogenase are key enzymes involved in bile acid metabolism. They catalyze the epimerization of a hydroxyl group through 7-keto bile acid intermediates. Basic research of the two enzymes has focused on exploring new enzymes and the structure-function relationship. The application research focused on the in vitro biosynthesis of bile acid drugs and the exploration and improvement of their catalytic ability based on molecular engineering. This article summarized the primary and advanced structural characteristics, specificities, biochemical properties, and applications of the two enzymes...
August 16, 2021: Protein and Peptide Letters
https://read.qxmd.com/read/34327850/the-catalytic-activity-of-mycelial-fungi-towards-7-oxo-dhea-an-endogenous-derivative-of-steroidal-hormone-dehydroepiandrosterone
#16
JOURNAL ARTICLE
Paulina Łyczko, Anna Panek, Ireneusz Ceremuga, Alina Świzdor
Seventeen species of fungi belonging to thirteen genera were screened for the ability to carry out the transformation of 7-oxo-DHEA (7-oxo-dehydroepiandrosterone). Some strains expressed new patterns of catalytic activity towards the substrate, namely 16β-hydroxylation (Laetiporus sulphureus AM498), Baeyer-Villiger oxidation of ketone in D-ring to lactone (Fusicoccum amygdali AM258) and esterification of the 3β-hydroxy group (Spicaria divaricata AM423). The majority of examined strains were able to reduce the 17-oxo group of the substrate to form 3β,17β-dihydroxy-androst-5-en-7-one...
July 29, 2021: Microbial Biotechnology
https://read.qxmd.com/read/34250527/characterisation-of-a-hyperthermophilic-transketolase-from-thermotoga-maritima-dsm3109-as-a-biocatalyst-for-7-keto-octuronic-acid-synthesis
#17
JOURNAL ARTICLE
Max Cárdenas-Fernández, Fabiana Subrizi, Dragana Dobrijevic, Helen C Hailes, John M Ward
Transketolase (TK) is a fundamentally important enzyme in industrial biocatalysis which carries out a stereospecific carbon-carbon bond formation, and is widely used in the synthesis of prochiral ketones. This study describes the biochemical and molecular characterisation of a novel and unusual hyperthermophilic TK from Thermotoga maritima DSM3109 (TKtmar). TKtmar has a low protein sequence homology compared to the already described TKs, with key amino acid residues in the active site highly conserved. TKtmar has a very high optimum temperature (>90 °C) and shows pronounced stability at high temperature (e...
July 12, 2021: Organic & Biomolecular Chemistry
https://read.qxmd.com/read/34201735/plasma-metabolomics-for-discovery-of-early-metabolic-markers-of-prostate-cancer-based-on-ultra-high-performance-liquid-chromatography-high-resolution-mass-spectrometry
#18
JOURNAL ARTICLE
Xiangping Lin, Lucie Lécuyer, Xinyu Liu, Mohamed N Triba, Mélanie Deschasaux-Tanguy, Aïcha Demidem, Zhicheng Liu, Tony Palama, Adrien Rossary, Marie-Paule Vasson, Serge Hercberg, Pilar Galan, Philippe Savarin, Guowang Xu, Mathilde Touvier
BACKGROUND: The prevention and early screening of PCa is highly dependent on the identification of new biomarkers. In this study, we investigated whether plasma metabolic profiles from healthy males provide novel early biomarkers associated with future risk of PCa. METHODS: Using the Supplémentation en Vitamines et Minéraux Antioxydants (SU.VI.MAX) cohort, we identified plasma samples collected from 146 PCa cases up to 13 years prior to diagnosis and 272 matched controls...
June 23, 2021: Cancers
https://read.qxmd.com/read/33972354/xre-type-regulator-biox-acts-as-a-negative-transcriptional-factor-of-biotin-metabolism-in-riemerella-anatipestifer
#19
JOURNAL ARTICLE
Xiaomei Ren, Zongchao Chen, Pengfei Niu, Wenlong Han, Chan Ding, Shengqing Yu
Biotin is essential for the growth and pathogenicity of microorganisms. Damage to biotin biosynthesis results in impaired bacterial growth and decreased virulence in vivo However, the mechanisms of biotin biosynthesis in Riemerella anatipestifer remain unclear. In this study, two R. anatipestifer genes associated with biotin biosynthesis were identified. AS87_RS05840 encoded a BirA protein lacking the N-terminal winged helix-turn-helix DNA binding domain, identifying it as a Group I biotin protein ligase, and AS87_RS09325 encoded a BioX protein, which was in the helix-turn-helix xenobiotic response element family of transcription factors...
May 10, 2021: Journal of Bacteriology
https://read.qxmd.com/read/33607268/11%C3%AE-hydroxysteroid-dehydrogenases-a-growing-multi-tasking-family
#20
JOURNAL ARTICLE
Elise P Gomez-Sanchez, Celso E Gomez-Sanchez
This review briefly addresses the history of the discovery and elucidation of the three cloned 11β-hydroxysteroid dehydrogenase (11βHSD) enzymes in the human, 11βHSD1, 11βHSD2 and 11βHSD3, an NADP+ -dependent dehydrogenase also called the 11βHSD1-like dehydrogenase (11βHSD1L), as well as evidence for yet identified 11βHSDs. Attention is devoted to more recently described aspects of this multi-functional family. The importance of 11βHSD substrates other than glucocorticoids including bile acids, 7-keto sterols, neurosteroids, and xenobiotics is discussed, along with examples of pathology when functions of these multi-tasking enzymes are disrupted...
February 16, 2021: Molecular and Cellular Endocrinology
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