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Yanzhi Luo, Wei Yu, Yongyao Yu, Shuai Dong, Yaxing Yin, Zhenyu Huang, Xinyu Wan, Liqiang Zhang, Yunzhen Yu, Taoshan Ai, Qingchao Wang, Zhen Xu
In mammalian, T-cell receptors (TCRs) play a key role in recognizing the presented antigen from external to protect organisms against environmental pathogens. To understand the potential roles of TCRγ and TCRδ in dojo loach (Misgurnus anguillicaudatus), Ma-TCRγ and Ma-TCRδ cDNAs were cloned and their gene expression profiles were investigated after bacterial, parasitic and fungal challenge. The open reading frame (ORF) of Ma-TCRγ and Ma-TCRδ cDNAs contained 948 and 867 bp, encoding 316 and 288 amino acid residues, respectively...
November 25, 2018: Fish & Shellfish Immunology
Yi He, Bin Men, Xiaofang Yang, Yaxuan Li, Hui Xu, Dongsheng Wang
Organic matter (OM) is an important component of sediment. Bioturbation/bioirrigation can remobilize OM and heavy metals that were previously buried in the sediment. The remobilization of buried organic matter, thallium (Tl), cadmium (Cd), copper (Cu) and zinc (Zn) from sediment was studied in a laboratory experiment with three organisms: tubificid, chironomid larvae and loach. Results showed that bioturbation/bioirrigation promoted the release of dissolved organic matter (DOM) and dissolved Tl, Cd, Cu and Zn, but only dissolved Zn concentrations decreased with exposure time in overlying water...
January 2019: Journal of Environmental Sciences (China)
George Shigueki Yasui, Taiju Saito, Yan Zhao, Takafumi Fujimoto, Etsuro Yamaha, Katsutoshi Arai
SummaryPolyspermy was initiated by microinjecting a multiple number of sperm into the activated and dechorionated eggs of dojo loach Misgurnus anguillicaudatus (Teleostei: Cobitidae). A 10-nl sperm suspension from an albino (recessive trait) male (105, 106, 107 or 108 sperm ml -1) was microinjected into eggs from a wild-type female. Although the rates of embryos developing into the blastula stage in the injection group at the highest sperm concentration were similar to that of the control group, the hatching rates of the injection group were much lower...
October 29, 2018: Zygote: the Biology of Gametes and Early Embryos
Xiaoyao Chen, Weiguang Kong, Yongyao Yu, Shuai Dong, Zhenyu Huang, Wei Yu, Jie Xu, Yanzhi Luo, Qingchao Wang, Zhen Xu
Interleukin 15 (IL15) is a pleiotropic cytokine that participates in innate and adaptive immunity along with its receptor α-chain (IL15Rα). In order to investigate the potential roles of IL15 and IL15Rα in dojo loach (Misgurnus anguillicaudatus), we firstly cloned the cDNA sequence of Ma-IL15 and Ma-IL15Rα, which contain 1096bp and 1236bp and code proteins of 193 amino acids and 210 amino acids, respectively. A short signal peptide and Pfam IL15 domain were found in Ma-IL15, while a highly conserved sushi domain existed in Ma-IL15Rα...
November 2018: Molecular Immunology
Liang Cao, E Zhang
Schistura alboguttata, a new species of nemacheilid loach, is herein described from the Leli-He, a tributary flowing to the You-Jiang of the Pearl River basin (Zhu-Jiang in Chinese) at Tianlin County, Guangxi, South China. This new species can be readily distinguished from all other Chinese species of Schistura by its striking body coloration consisting of irregular white spots scattered over the dorsal and lateral regions of the body, with occasional irregular bars with narrow interspaces on the predorsal region...
September 4, 2018: Zootaxa
Anabel Perdices, Cevher S Ozeren, Füsun Erkakan, Jörg Freyhof
Accurate determination of species diversity in areas of high endemicity, particularly those lacking comprehensive systematic knowledge, represents a challenge for both taxonomists and conservationists. This need is particularly evident in areas greatly affected by anthropogenic disturbances such as the Eastern Mediterranean and its freshwater environments. To improve our knowledge of Eastern Mediterranean freshwater fishes, we phylogenetically studied Western Palearctic Cobitis species, focusing on those found in Turkey...
2018: PloS One
Ming Zhu, Shengjun Wu
Four diets supplemented with three different levels of β-1,3-glucan (0.5, 1, and 2 g kg-1 ) were formulated and tested for growth, intestine digestive-enzyme activities, PO, SOD and GPx activities and erythrocytes number, phagocytic percentage of erythrocytes and lysozyme activity and resistance to Aeromonas hydrophila of loach Paramisgurnus dabryanus. A diet without any β-1,3-glucan served as the control. After 60 days of feeding, the growth performance, intestine digestive-enzyme activities, nonspecific immunity, and resistance to Aeromonas hydrophila of P...
December 2018: Fish & Shellfish Immunology
Yanjie Qin, Xia Li, Yanjin Yang, Zhuangzhuang Li, Yan Liang, Xiaoyu Zhang, Shan Jiang
The effects of different concentrations of copper sulfate on diploid and triploid fin cell lines (named DIMF and TRMF, respectively) in Misgurnus anguillicaudatus were studied. The LC50 of copper sulfate estimated by an MTT assay was 268.39 in DIMF cells, and 311.54 μmol/L in TRMF cells, respectively. Activity of superoxide dismutase (SOD) in DIMF cells gradually increased as the concentration of copper sulfate increased (up to 200 μmol/L), and then gradually decreased. SOD activity in triploid loach fin cells, as well as glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activity in both diploid and triploid cells, decreased as the concentration of copper sulfate increased, which suggested that excessive copper exposure at the concentrations tested in this study was detrimental to anti-oxidative capability...
December 1, 2018: Science of the Total Environment
V K Anoop, Neelesh Dahanukar, Siby Philip, Liju Thomas, Rajeev Raghavan
Rivers draining the Western Ghats (WG) mountain ranges in peninsular India harbor an exceptionally diverse, unique and evolutionarily distinct assemblage of lower vertebrates with high levels of endemism, attributed to their evolution and potentially long history of isolation during the Late Cretaceous or Early Tertiary. A molecular phylogeny of hillstream loaches of the genus Mesonoemacheilus endemic to the WG revealed the presence of four clades which we designate as 'species groups'. A consensus of various species delimitation methods indicates the likelihood of 'at least' seven more undescribed species within Mesonoemacheilus...
December 2018: Molecular Phylogenetics and Evolution
Annette Jaya-Ram, Farhana Fuad, Mohd Shafiq Zakeyuddin, Amir Shah Ruddin Md Sah
One of the oldest reservoirs in Peninsular Malaysia, Bukit Merah Reservoir, is a place in which locals participate in fishing activities. Inland fisheries are important to individuals, society and the environment; whereby they generate a source of income and food security. It is essential to gauge the nutrition value of fish caught in this location as food source, especially in terms of fatty acid composition, to better demonstrate its potential towards the betterment of human health and general well-being...
July 2018: Tropical Life Sciences Research
Han Qu, Ruixue Ma, Fang Wang, Jing Gao, Peng Wang, Zhiqiang Zhou, Donghui Liu
In this work, the enantioselective toxicity, enrichment, and distribution of fipronil and its main metabolites (fipronil sulfone, fipronil sulfide, and fipronil desulfinyl) in loach (Misgurnus anguillicaudatus) were studied. The influence of maize-straw derived biochar on acute toxicity and bioaccumulation of contaminants were also investigated. The three main metabolites were more toxic to loach than parent fipronil. Meanwhile, loach exhibited more sensitive to S-enantiomer. The alleviated toxic response of loach was observed in the presence of biochar during 72-h acute toxicity test...
October 15, 2018: Journal of Hazardous Materials
Shaokui Yi, Xiaoyun Zhou, Jie Li, Manman Zhang, Shuangshuang Luo
Reconstruction and annotation of transcripts, particularly for a species without reference genome, plays a critical role in gene discovery, investigation of genomic signatures, and genome annotation in the pre-genomic era. This study generated 33,330 full-length transcripts of diploid M. anguillicaudatus using PacBio SMRT Sequencing. A total of 6,918 gene families were identified with two or more isoforms, and 26,683 complete ORFs with an average length of 1,497 bp were detected. Totally, 1,208 high-confidence lncRNAs were identified, and most of these appeared to be precursor transcripts of miRNAs or snoRNAs...
August 3, 2018: Scientific Reports
Dominik K Kölmel, Richard P Loach, Thomas Knauber, Mark E Flanagan
A new procedure for the photoredox-mediated conjugate addition of radicals that can be conveniently generated from α-amino acids to DNA-tagged Michael acceptors and styrenes is presented. This C(sp3 )-C(sp3 ) coupling tolerates a broad array of structurally diverse radical precursors, including all of the 20 proteinogenic amino acids. Importantly, this reaction proceeds under mild conditions and in DNA-compatible aqueous media. Furthermore, the presented reaction conditions are compatible with DNA, making this reaction platform well suited for the construction of DNA-encoded libraries...
July 31, 2018: ChemMedChem
Yuichi Kano, Jun Nakajima, Takeshi Yamasaki, Jyun-Ichi Kitamura, Ryoichi Tabata
Background: Loach is one of the major cypriniform fishes in freshwater habitats of Japan; 35 taxa/clades have, until now, been recognised. Parallel to genetic studies, morphological examinations are needed for further development of loach study, eventually ichthyology and fish biology. Digital archiving, concerning taxonomy, ecology, ethology etc., is one of the progressive challenges for the open science of biology. This paper aimed to online publish photo images, 3D models and CT scanned data of all the known clades of loaches inhabiting Japan (103 individuals in total with several type specimens), contributing to ichthyology and public interest of biodiversity/biology...
2018: Biodiversity Data Journal
S Shamsi, A Stoddart, L Smales, S Wassens
There is a paucity of information on the diversity and occurrence of freshwater fish parasites in Australia. This study investigates the distribution and occurrence of a parasitic nematode of the genus Contracaecum in freshwater fish from south-eastern Australia. Fish (n = 508) belonging to nine species and eight families were collected from eight wetlands associated with the Murrumbidgee River floodplain in the southern Murray-Darling Basin and subjected to laboratory examination. Third-stage nematode larvae were found in eight of the nine fish species...
July 18, 2018: Journal of Helminthology
Han Qu, Ruixue Ma, Bin Wang, Yizhe Zhang, Lina Yin, Gang Yu, Shubo Deng, Jun Huang, Yujue Wang
In this study, we investigated the enantioselective environmental behaviors of the chiral antidepressant venlafaxine (VFX) in lab-scale aquatic ecosystems in the presence of microplastics (MPs). To determine the bioaccumulation, distribution, and metabolism as well as the effects of MPs on aquatic ecosystems, water-sediment, water-Lemna.minor (L.minor), water-Misgurnus.anguillicaudatus (M.anguillicaudatus), and water-sediment-L.minor-M.anguillicaudatus ecosystems were set up and exposed to venlafaxine and two levels of microplastics over a 90-day period...
October 5, 2018: Journal of Hazardous Materials
Masamichi Kuroda, Takafumi Fujimoto, Masaru Murakami, Etsuro Yamaha, Katsutoshi Arai
Wild-type dojo loach (Misgurnus anguillicaudatus) commonly reproduces bisexually as a gonochoristic diploid (2n = 50), but gynogenetically reproducing clonal diploid lines (2n = 50) exist in certain districts in Japan. Clones have been considered to develop from past hybridization event(s) between two genetically diverse groups, A and B, within the species. Fluorescence in situ hybridization analyses using the repetitive sequence "ManDra" as a probe clearly distinguished 25 chromosomes derived from group B out of a total of 50 diploid chromosomes of the clone, providing strong molecular cytogenetic evidence of its hybrid origin...
June 7, 2018: Chromosome Research
He Zhou, Qi-Zheng Xu, Rui Zhang, Zi-Xin Zhuang, Yin-Qiang Ma, Wei Wang, Tian-Yu Ma, Yi Sui, Yang Liu, Xiaojuan Cao
Hybrid triploid loaches (Misgurnus anguillicaudatus) were generated from natural tetraploid and diploid loaches. We studied the gonads of parents and offspring from direct and reciprocal crosses through histological and transcriptome analyses. The triploid offspring had inferior ability to form sperm and egg cells compared with diploid forms. After sequencing the transcriptomes, 655,109,158 clean reads were obtained, and 62,821 unigenes and 178,962 transcripts were assembled. Of these unigenes, 23,189 were annotated in the GO database, 18,525 in the KEGG database and 24,661 in the KOG database...
2018: PloS One
J J Kim, Y K Nam, I C Bang, S P Gong
  BACKGROUND: Miho spine loach (Cobitis choii) is an endangered Korean endemic fish. Whole testis cryopreservation is a good way for species preservation, but needs to the sacrifice of a large number of fish to optimize the freezing condition. Considering this limitation, a surrogate fish species was used for the protocol development. OBJECTIVE: This study was to establish the effective condition for Miho spine loach whole testis cryopreservation by optimizing the conditions for whole testis cryopreservation in an allied species, mud loach (Misgurnus mizolepis)...
January 2018: Cryo Letters
Jingwen Chen, Yun Cui, Jie Yan, Jimin Jiang, Xiaojuan Cao, Jian Gao
Elongase of very long-chain fatty acids 6 (ELOVL6) is a rate-limiting enzyme catalyzing elongation of saturated and monounsaturated long-chain fatty acid. Although functional characteristics of Elovl6 have been demonstrated in mammal, the role of elovl6 in fish remains unclear. In this study, we firstly cloned three isoforms of elovl6 (elovl6a, elovl6b and elovl6-like) from loach (Misgurnus anguillicaudatus). Molecular characterizations of the three elovl6 isoforms in loach and their expressions of early life stages and different tissues were then determined...
August 5, 2018: Gene
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