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Platelet mapping

Kaipu Jin, Heqing Wang, Mengsu Zeng, Shengxiang Rao, Lixia Yan, Yuan Ji, Caixia Fu, Ruofan Sheng
OBJECTIVE: To evaluate the value of MR liver extracellular volume (ECVliver ) in assessment of liver fibrosis with chronic hepatitis B (CHB), and to compare its performance with two-dimensional (2D) shear-wave elastography (SWE). MATERIALS AND METHODS: A total of 68 CHB patients who were histologically diagnosed as fibrosis stages F0 to F4 were retrospectively analyzed. All patients underwent gadopentetate dimeglumine-enhanced T1-mapping and 2D SWE. ECVliver and liver stiffness were measured and compared between fibrosis subgroups; their correlations with histologic findings were evaluated using Spearman correlation test and multiple regression analysis...
December 7, 2018: Abdominal Radiology
José Juan-Colás, Ian S Hitchcock, Mark Coles, Steven Johnson, Thomas F Krauss
Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution...
December 10, 2018: Proceedings of the National Academy of Sciences of the United States of America
Lindsay S Hall, Charlotte S Lennon, Andrew M Hall, Stanislaw J Urbaniak, Mark A Vickers, Robert N Barker
Platelet destruction in immune thrombocytopenia is caused by autoreactive antibody and T cell responses, most commonly directed against platelet glycoprotein IIb/IIIa. Loss of self-tolerance in the disease is also associated with deficient activity of regulatory T cells. Having previously mapped seven major epitopes on platelet glycoprotein IIIa that are recognised by helper T cells from patients with immune thrombocytopenia, the aim was to test whether peptide therapy with any of these sequences, alone or in combination, could inhibit responses to the antigen in humanized mice expressing HLA-DR15...
December 4, 2018: Haematologica
Khairul Syahputra, Per W Kania, Azmi Al-Jubury, Rzgar M Jafaar, Ron P Dirks, Kurt Buchmann
The parasite Ichthyophthirius multifiliis infecting skin, fins and gills of a wide range of freshwater fish species, including rainbow trout, is known to induce a protective immune response in the host. Although a number of studies have reported activation of several immune genes in infected fish host, the immune response picture is still considered incomplete. In order to address this issue, a comparative transcriptomic analysis was performed on infected versus uninfected rainbow trout gills and it showed that a total of 3352 (7...
December 1, 2018: Fish & Shellfish Immunology
Fernando M Berganza, Cesar Gonzalez de Alba, Alexander C Egbe, Sergio Bartakian, John Brownlee
RationaleAspirin resistance has been reported in up to 80% of children with cardiovascular defects undergoing surgery. Because of a patient who had embolic stroke while on therapeutic aspirin dose but in whom aspirin resistance was present on his thromboelastography platelet mapping, we chose to obtain thromboelastography platelet mapping on cardiac patients on aspirin to assess their risk. OBJECTIVES: This study evaluates aspirin resistance noted in these patients and their characteristics.Methods and resultsThis is a retrospective study of 25 patients taking aspirin for a month at therapeutic dose...
December 3, 2018: Cardiology in the Young
Shuang Zhou, Vicky A Appleman, Christopher M Rose, Hyun Jung Jun, Juechen Yang, Yue Zhou, Roderick T Bronson, Steve P Gygi, Al Charest
Activation of the platelet-derived growth factor receptors (PDGFRs) gives rise to some of the most important signaling pathways that regulate mammalian cellular growth, survival, proliferation, and differentiation and their misregulation is common in a variety of diseases. Herein, we present a comprehensive and detailed map of PDGFR signaling pathways assembled from literature and integrate this map in a bioinformatics protocol designed to extract meaningful information from large-scale quantitative proteomics mass spectrometry data...
June 2018: Life science alliance
Katharina Seif, Lejla Alidzanovic, Barbara Tischler, Nahla Ibrahim, Branislav Zagrapan, Sabine Rauscher, Manuel Salzmann, Lena Hell, Lisa-Marie Mauracher, Ulrich Budde, Johannes A Schmid, Bernd Jilma, Ingrid Pabinger, Alice Assinger, Patrick Starlinger, Christine Brostjan
Thrombospondin-1 (TSP-1) is primarily expressed by platelets and endothelial cells (ECs) and rapidly released upon their activation. It functions in haemostasis as a bridging molecule in platelet aggregation, by promoting platelet adhesion to collagen and by protecting von Willebrand factor strings from degradation. In blood of patients undergoing surgery and in co-cultures of neutrophils with platelets or ECs, we observed proteolysis of the 185 kDa full-length TSP-1 to a 160-kDa isoform. We hypothesized that TSP-1 processing may alter its haemostatic properties...
November 12, 2018: Thrombosis and Haemostasis
Stefan Skalsky, John Molloy, Mira Naftaly, Toby Sainsbury, Keith R Paton
Few-layer graphene (FLG) platelets exfoliated directly from graphite are finding a wide range of potential applications, including composites and printed electronics. However, characterisation of the FLG material following incorporation into polymers, including the quality of the dispersion, remains a challenge. Here, we present the use of terahertz time-domain spectroscopy as a potential solution to this challenge which could form the basis of a rapid characterisation tool. The THz refractive index was found to be highly sensitive to the loading of FLG, opening the route to mapping local FLG concentration within a polymer composite sample...
January 11, 2019: Nanotechnology
Sonia Salha, Sebastian Gehmert, Vanessa Brébant, Alexandra Anker, Markus Loibl, Lukas Prantl, Sanga Gehmert
INTRODUCTION: Mesenchymal stem cells (MSCs) have been described in breast cancer models to migrate towards carcinoma and integrate into tumor associated stroma supporting tumor growth, increasing their metastatic potency and contributing to tumor-angiogenesis. Platelet-derived growth factor (PDGF) isoforms (AA, BB, CC) stimulate growth, survival and motility of MSCs and certain other cell types. Noteworthy, breast carcinomas are known to express PDGF. We aim to further shed light on i) the relevance of the different PDGF isoforms on adipose tissue derived stem cells (ASCs) migration and ii) the underlying pathway dependent on PDGF stimulation...
October 19, 2018: Clinical Hemorheology and Microcirculation
John S Kim, Jon Kaufman, Sonali S Patel, Marilyn Manco-Johnson, Jorge Di Paola, Eduardo M da Cruz
BACKGROUND: Ketorolac is used for pediatric analgesia after surgery despite its known platelet inhibition via the arachidonic acid (AA) pathway. The degree of platelet inhibitory effect after cardiac surgery is not well characterized. Thromboelastography with platelet mapping (TEG-PM) is emerging as a frequently used test to evaluate platelet inhibition via the AA pathway. METHODS: Post hoc analysis of a data set collected in a prospective observational cohort study evaluating platelet inhibition in children after congenital heart surgery with cardiopulmonary bypass (CPB)...
November 2018: World Journal for Pediatric & Congenital Heart Surgery
Ruixia Song, Chenming Dong, Cuiting Wang, Hong Zhang, Zhaohui Yang
OBJECTIVE: To systematical evaluate the effect of Xuebijing injection in the treatment of multiple organ dysfunction syndrome (MODS). METHODS: With the keywords including Xuebijing, multiple organ dysfunction syndrome, multiple organ dysfunction and multiple organ failure, PubMed, the Cochrane Library, Embase, China National Knowledge Infrastructure (CNKI), China Biology Medicine (CBM), VIP and Wanfang Data from the database start until March 4th, 2018 were searched for relevant randomized controlled trials (RCTs) related to Xuebijing injection combined conventional treatment versus conventional treatment alone for MODS...
September 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Aaron Seo, Suleyman Gulsuner, Sarah Pierce, Miri Ben-Harosh, Hanna Shalev, Tom Walsh, Tanya Krasnov, Orly Dgany, Sergei Doulatov, Hannah Tamary, Akiko Shimamura, Mary-Claire King
Severe thrombocytopenia, characterized by dysplastic megakaryocytes and intracranial bleeding, was diagnosed in six individuals from a consanguineous kindred. Three of the individuals were successfully treated by bone marrow transplant. Whole exome sequencing and homozygosity mapping of multiple family members, coupled with whole genome sequencing to reveal shared non-coding variants, revealed one potentially functional variant segregating with thrombocytopenia under a recessive model: GALE p.R51W (c.C151T, NM_001127621)...
September 20, 2018: Human Molecular Genetics
Lindsay J May, Angela Lorts, Christina VanderPluym, Jennifer Conway, M Patricia Massicotte, Morgan M Millar, Marie E Steiner, David N Rosenthal
Management of antithrombotic therapy (ATT) for pediatric ventricular assist devices is challenging, and the Berlin EXCOR remains the only FDA-approved option. Among those on the EXCOR, 28% have neurologic complications and major bleeding occurs in 50%. The Edmonton Protocol was developed to guide ATT, but the adverse event rate remains high, leading most centers to make modifications. The objective of this study is to characterize antithrombotic practice variation among North American pediatric ventricular assist device programs, in order to guide future research...
September 12, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Kodai Uematsu, Yukiko Enomoto, Takashi Onuma, Masanori Tsujimoto, Tomoaki Doi, Rie Matsushima-Nishiwaki, Haruhiko Tokuda, Shinji Ogura, Hiroki Iida, Osamu Kozawa, Toru Iwama
BACKGROUND/AIMS: Thrombin induces the activation of human platelets through protease-activated receptor (PAR) 1 and PAR4, and Rac, a member of the Rho family of small GTPases, is implicated in PAR activation. We previously reported that phosphorylated-heat shock protein 27 (HSP27) is released from the thrombin receptor-activating peptide (TRAP)-stimulated platelets of diabetic patients. In the present study, we investigated the role of Rac in the TRAP-elicited release of phosphorylated-HSP27 from human platelets...
2018: Cellular Physiology and Biochemistry
Chomaw Sitotaw, Fikir Asrie, Mulugeta Melku
OBJECTIVE: To evaluate platelet and White cell parameters in women with preeclampsia (PE) in comparison with a healthy pregnancy. METHODS: A cross-sectional study was carried in 2015 at University of Gondar hospital. Thirty-three mild PE, 30 severe PE cases and 63 healthy pregnant women were enrolled in the study. About 3 mL venous blood sample was collected from each study participants. Hematological parameters were determined by Sysmex KX-21 hematological analyzer...
July 2018: Pregnancy Hypertension
Shane A Shapiro, Jennifer R Arthurs, Michael G Heckman, Joseph M Bestic, Shari E Kazmerchak, Nancy N Diehl, Abba C Zubair, Mary I O'Connor
Objective Bone marrow aspiration and concentration (BMAC) is becoming a more common regenerative therapy for musculoskeletal pathology. In our current pilot study, we studied patients with mild-to-moderate bilateral knee osteoarthritis, compared pain at 12-month follow-up between BMAC-injected and saline-injected knees, and examined cartilage appearance measured by magnetic resonance imaging (MRI) T2 quantitative mapping. Design Twenty-five patients with mild-to-moderate bilateral osteoarthritic knee pain were randomized to receive BMAC into one knee and saline placebo into the other...
August 30, 2018: Cartilage
Sante E I Carone, Danilo L Menaldo, Marco A Sartim, Carolina P Bernardes, Renato C Caetano, Ronivaldo R da Silva, Hamilton Cabral, Benedito Barraviera, Rui S Ferreira Junior, Suely V Sampaio
Snake venom serine proteases (SVSPs) are commonly described as capable of affecting hemostasis by interacting with several coagulation system components. In this study, we describe the isolation and characterization of BjSP from Bothrops jararaca snake venom, a serine protease with distinctive properties. This enzyme was isolated by three consecutive chromatographic steps and showed acidic character (pI 4.4), molecular mass of 28 kDa and N-carbohydrate content around 10%. Its partial amino acid sequence presented 100% identity to a serine protease cDNA clone previously identified from B...
August 24, 2018: Toxicology and Applied Pharmacology
Michael Caspers, Nadine Schäfer, Matthias Fröhlich, Ursula Bauerfeind, Bertil Bouillon, Manuel Mutschler, Marc Maegele
BACKGROUND: External factors following trauma and iatrogenic intervention influence blood coagulation and particularly clot formation. In particular, three external factors (in detail dilution via uncritical volume replacement, acidosis and hypothermia), in combination, referred to as the "lethal triad", substantially aggravate the hypocoagulative state after trauma. Contribution of these external factors to the resulting hypocoagulative state in trauma and especially their influence on primary haemostasis has still not been investigated systematically...
August 15, 2018: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
Hong Li, Manuel Zeitelhofer, Ingrid Nilsson, Xicong Liu, Laura Allan, Benjamin Gloria, Angelo Perani, Carmel Murone, Bruno Catimel, A Munro Neville, Fiona E Scott, Andrew M Scott, Ulf Eriksson
PDGF-CC is a member of the platelet-derived growth factor (PDGF) family that stimulates PDGFRα phosphorylation and thereby activates intracellular signalling events essential for development but also in cancer, fibrosis and neuropathologies involving blood-brain barrier (BBB) disruption. In order to elucidate the biological and pathological role(s) of PDGF-CC signalling, we have generated high affinity neutralizing monoclonal antibodies (mAbs) recognizing human PDGF-CC. We determined the complementarity determining regions (CDRs) of the selected clones, and mapped the binding epitope for clone 6B3...
2018: PloS One
Rachael Morris, Shauna-Kay Spencer, Allison Barnes, Teylor Bowles, Patrick B Kyle, Kedra Wallace
HELLP (hemolysis elevated liver enzyme low platelet) syndrome is associated with hypertension, inflammation, oxidative stress and endothelial activation. The objective of this study was to determine if oxygen scavenging or endothelin A receptor antagonism improved hypertension and oxidative stress. sFlt-1 and sEndoglin were infused via mini-osmotic pump into normal pregnant rats (NP) on gestational day 12 to create HELLP syndrome. On gestational day 18 arterial catheters were inserted and on gestational day 19 mean arterial pressure was analyzed in rats; serum, urine and tissues were collected for molecular analysis...
September 5, 2018: European Journal of Pharmacology
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