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tumor chemosensitivity assay

Hongmei Zhu, Shu Ruan, Feng Jia, Jiusheng Chu, Yong Zhu, Yongjiu Huang, Guan Liu
Background: Berbamine (BBM), one of the bis-benzylisoquinoline products isolated from Berberis amurensis , has been demonstrated for its anticancer effect against leukemia, breast cancer, liver cancer, etc. There are some studies focusing on the chemosensitization effect of BBM. However, there is no report about whether BBM could enhance the anticancer effect of radiation, which made us to explore the possible radiosensitization effect of BBM. Materials and methods: Here, in vitro cytotoxicity of BBM was evaluated on two kinds of head and neck squamous cancer cell lines...
2018: OncoTargets and Therapy
Wei Sun, Wei Ping, Yitao Tian, Wenbin Zou, Jiawei Liu, Yukun Zu
BACKGROUND/AIMS: KRas is usually mutated in non-small cell lung cancer (NSCLC). The mutated KRas gene is a negative prognostic indicator that promotes tumor proliferation, metastasis, and drug resistance in NSCLC, and thus has become a target for cancer therapy. This study is focused on the effects of the microRNA (miR)-202/KRas axis in regulating chemosensitivity in NSCLC. METHODS: Quantitative reverse transcriptase real-time PCR analysis was performed to examine the expression of miR-202...
2018: Cellular Physiology and Biochemistry
Suat Erdogan, Oguzhan Doganlar, Zeynep B Doganlar, Kader Turkekul
Background: The aim of the study was to evaluate whether the use of chemotherapy in combination with naringin, a dietary plant polyphenolic flavonoid, could enhance the therapeutic efficacy of paclitaxel treatment in human prostate cancer (PCa) cells. Materials and methods: DU145, PC3, and LNCaP cells were treated with various concentrations of paclitaxel, naringin, and their combinations. Methylthiazolyldiphenyl-tetrazolium bromide (MTT), image-based cytometer, quantitative reverse transcription PCR (RT-qPCR), Western blot, and transwell assay were used to evaluate cell viability, apoptosis and cell cycle, the mRNA expression, protein expression, and cell migration, respectively...
December 2018: Prostate International
Jeong Yong Moon, Le Van Manh Hung, Tatsuya Unno, Somi Kim Cho
Drug resistance is a major problem in the treatment of non-small-cell lung cancer (NSCLC). In this study, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed to identify the differentially expressed genes in Adriamycin (ADR)-resistant NSCLC A549/ADR cells compared with parental A549 cells. Among the tested phytochemicals, nobiletin (NBT) is able to overcome the ADR resistance of A549/ADR cells. NBT treatment decreased the expression of a neuroblastoma-derived MYC (MYCN) and multidrug resistance-associated protein 1 (MRP1) as well as downregulating Akt, GSK3β, and β-catenin...
November 26, 2018: Nutrients
Gerhard Hamilton, Barbara Rath
Drug screening assays employing 2D-cultures of cancer cells have been largely replaced by 3D multicellular tumor spheroid (MCTS) models which more closely represent patient's tumors. The predictive power of the different MCTSs depends on source of the cells, techniques of preparation and characteristics of the aggregates. Areas covered: The preparation of MCTSs and a comparison of the spheroids assembled from permanent cancer and patient-derived cell lines in respect to the correlation of their chemosensitivity to clinical responses are discussed...
November 28, 2018: Expert Opinion on Drug Metabolism & Toxicology
Lisi Zeng, Quanxing Liao, Zhaowei Zou, Yuefeng Wen, Jingshu Wang, Chang Liu, Qingjun He, Nuoqing Weng, Judeng Zeng, Hongsheng Tang, Runya Fang, Ziying Lei, Zhen Tang, Xianzi Yang, Shuzhong Cui
BACKGROUND/AIMS: The development of multidrug resistance (MDR), which results in disease recurrence and metastasis, is a crucial obstacle to successful chemotherapy for patients with gastric cancer (GC). Long non-coding RNAs (lncRNAs) have been found to play various roles in cancer. This study aimed to investigate the effect of XLOC_006753 on the development of MDR in GC cells. METHODS: The expression levels of XLOC_006753 in GC patients and MDR GC cell lines (SGC-7901/5-FU and SGC-7901/DDP cell line) were assessed by qRT-PCR...
November 27, 2018: Cellular Physiology and Biochemistry
Xiao-Long Hu, Jia-Hao Feng, Thi-Anh Pham, Hai-Yan Ma, Ming-Xi Ma, Rui Song, Wei Shen, Fei Xiong, Xiao-Qi Zhang, Wen-Cai Ye, Hao Wang
BACKGROUND: Nuclear protein poly (ADP-ribose) polymerase-1 (PARP-1) is a key enzyme in the repair of DNA and is a promising target in the development of chemosensitizers. This study first investigated the inhibitory effects of amentoflavone (AMF) and its derivatives on PARP-1 and the potentiation of AMF on carboplatin (CBP) in non-small cell lung cancer (NSCLC). PURPOSE: This study aims to evaluate the inhibitory effect of AMF against PARP-1 and its potentiation on CBP in lung cancer both in vitro and in vivo...
November 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Lin Ye, Tingxiu Xiang, Jing Zhu, Dairong Li, Qin Shao, Weiyan Peng, Jun Tang, Lili Li, Guosheng Ren
BACKGROUND/AIMS: Interferon consensus sequence-binding protein 8 (IRF8) belongs to a family of interferon (IFN) regulatory factors that modulates various important physiological processes including carcinogenesis. As reported by others and our group, IRF8 expression is silenced by DNA methylation in both human solid tumors and hematological malignancies. However, the role of IRF8 in lung carcinoma remains elusive. In this study, we determined IRF8 epigenetic regulation, biological functions, and the signaling pathway involved in non-small cell lung cancer (NSCLC)...
2018: Cellular Physiology and Biochemistry
Longsheng Wang, Shaojun Chen, Ziwei Zhang, Junfeng Zhang, Shiyu Mao, Jiayi Zheng, Yang Xuan, Mengnan Liu, Keke Cai, Wentao Zhang, Yadong Guo, Wei Zhai, Xudong Yao
BACKGROUND: This study aimed to explore hyper-O-linked N-acetylglucosaminylation (O-GlcNAcylation) with an elevation of the expression of O-linked-β-N-acetylglucosamine transferase (OGT) in human bladder cancer. METHODS: Immunohistochemical staining for OGT and O-GlcNAcylation was performed in 20 paired human bladder cancer and adjacent normal tissues, as well as in human bladder cancer tissue microarrays (N = 169). The expression level of OGT and O-GlcNAcylation in cell lines were detected using the Western blot analysis...
November 20, 2018: BMC Cancer
Yan Zhang, Hongying Xu, Junhao Mu, Shuliang Guo, Lin Ye, Dairong Li, Weiyan Peng, Xiaoqian He, Tingxiu Xiang
Lung cancer is the most frequently diagnosed cancer worldwide. Epigenetic regulation contributes to lung cancer pathogenesis. The ADAMTS18 tumor suppressor gene is inactivated in some cancers, but its involvement in lung cancer has not been shown. Immunohistochemistry, quantitative reverse-transcription polymerase chain reaction (qRT-PCR), and methylation-specific PCR were used to assay ADAMTS18 expression and promoter methylation in lung tumor tissues and adjacent tissues. Cell viability, transwell, and wound-healing assays, as well as flow cytometry were used to characterize the biological activity of ADAMTS18...
November 11, 2018: Journal of Cellular Physiology
Irina Druzhkova, Nadezhda Ignatova, Natalia Prodanets, Nikolay Kiselev, Iliya Zhukov, Marina Shirmanova, Vladimir Zagainov, Elena Zagaynova
BACKGROUND: The conventional chemotherapy of colorectal cancer with irinotecan, 5-fluorouracil, and oxaliplatin remains one of the front-line treatments worldwide. However, its efficacy is quite low. Recently studies of the epithelial-mesenchymal transition (EMT) have become the focus of investigations into the cause of chemoresistance in several types of cancer, including colorectal cancer. The data about the role of EMT in chemosensitivity are controversial. MATERIALS AND METHODS: Human colon adenocarcinoma cell lines HT29 and HCT116 and 14 primary short-term cultures established from patient tumors were used...
October 16, 2018: Clinical Colorectal Cancer
Ke-Ting Que, Yun Zhou, Yu You, Zhen Zhang, Xiao-Ping Zhao, Jian-Ping Gong, Zuo-Jin Liu
BACKGROUND: MicroRNAs (miRNAs) posttranscriptionally regulate gene expression and thereby contribute to the modulation of numerous complex and disease-relevant cellular processes, including cell proliferation, cell motility, apoptosis and stress response. miRNA-31-5p is encoded on a genomic fragile site, 9p21.3, which is reportedly lost in many hepatocellular carcinoma (HCC) tumors. Based on previous findings, we hypothesized that miR-31-5p alters chemosensitivity and that miR-31-5p mimics may influence sensitivity to chemotherapeutics in HCC as well as in a variety of other cancers...
November 6, 2018: Journal of Experimental & Clinical Cancer Research: CR
Jing Jiang, Chuanmei Xie, Yujuan Liu, Qi Shi, Yan Chen
MicroRNAs are small non-coding RNAs which play important roles in tumor progression. MiR-383-5p has been characterized as a cancer suppressor in several cancers. The aim of theses present study was to explore the role of miR-383-5p in the proliferation and chemosensitivity of ovarian cancer cells. MiR-383-5p expression was down-regulated while the expression of TRIM27 was up-regulated in ovarian cancer tissues and cell lines. We came up with the hypothesis that miR-383-5p might be involved in the tumor progression and chemoresistance of ovarian cancer through targeting TRIM27...
November 3, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Xu Chen, Ruihui Xie, Peng Gu, Ming Huang, Jinli Han, Wen Dong, Weibin Xie, Bo Wang, Wang He, Guangzheng Zhong, Ziyue Chen, Jian Huang, Tianxin Lin
PURPOSE: Chemoresistance and tumor relapse are the leading cause of deaths in bladder cancer patients. Bladder cancer stem cells (BCSCs) have been reported contribute to these pathological properties. However, the molecular mechanisms underlying their self-renewal and chemoresistance remain largely unknown. In the current study, a novel lncRNA-LBCS has been identified and explored in BCSCs. EXPERIMENTAL DESIGN: Firstly, we establish BCSCs model and explore the BCSCs associated lncRNAs by transcriptome microarray...
November 5, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Ge Yang, Yang Fu, Xiaoyan Lu, Menghua Wang, Hongtao Dong, Qiuming Li
The present study aimed to explore the combined role of microRNA (miR)-34a, melanoma antigen-A (MAGE‑A) and p53 in altering the chemosensitivity of retinoblastoma (RB) cells. Human RB and adjacent tumor tissues, as well as human RB cell lines (HXO‑Rb44, SO‑Rb50, Y79 and WERI‑Rb-1) were used. In addition, four chemotherapeutic drugs, including carboplatin, etoposide, Adriamycin and vincristine, were used to treat the cell lines, in order to evaluate the sensitivity of RB cells. Furthermore, miR‑34a expression was detected by reverse transcription-quantitative polymerase chain reaction, and western blotting was implemented to quantify expression levels of MAGE‑A and p53...
October 31, 2018: International Journal of Oncology
Nobuyuki Tanaka, Shintaro Katayama, Aparna Reddy, Kaneyasu Nishimura, Naoya Niwa, Hiroshi Hongo, Koichiro Ogihara, Takeo Kosaka, Ryuichi Mizuno, Eiji Kikuchi, Shuji Mikami, Ayako Miyakawa, Ernest Arenas, Juha Kere, Mototsugu Oya, Per Uhlén
Cancers acquire resistance to systemic treatment with platinum-based chemotherapy (eg, cisplatin [CDDP]) as a result of a dynamic intratumoral heterogeneity (ITH) and clonal repopulation. However, little is known about the influence of chemotherapy on ITH at the single-cell level. Here, mapping the transcriptome of cancers treated with CDDP by scRNA-seq, we uncovered a novel gene, COX7B, associated with platinum-resistance, and surrogate marker, CD63. Knockdown of COX7B in cancer cells decreased the sensitivity of CDDP whereas overexpression recovered the sensitivity of CDDP...
October 26, 2018: Cancer Medicine
Wanyuan Chen, Weimin Fan, Guoqing Ru, Fang Huang, Xiaming Lu, Xin Zhang, Xiaozhou Mou, Shibing Wang
Pancreatic cancer (PC) is a lethal solid malignancy with resistance to traditional chemotherapy. Recently, considerable studies have demonstrated the ubiquitous antitumor properties of gene therapy mediated by the oncolytic vaccinia virus. The second mitochondrial‑derived activator of caspase (Smac) has been identified as an innovative tumor suppressor that augments the chemosensitivity of cancer cells. However, the therapeutic value of oncolytic vaccinia virus (oVV)‑mediated Smac gene transfer in pancreatic cancer is yet to be elucidated...
October 24, 2018: Oncology Reports
Chao Zhang, Lian-Jun He, Hai-Zhu Ye, Ding-Feng Liu, Yi-Bao Zhu, Dong-Dong Miao, Sheng-Peng Zhang, Yun-Yu Chen, Yuan-Wei Jia, Jie Shen, Xiao-Ping Liu
Multidrug resistance (MDR) is a major concern when using chemotherapy for the treatment of patients with colorectal cancer. MDR modulators are agents that can reverse MDR and, thus, enhance the chemosensitivity of tumor cells. The development of MDR modulators can improve the therapeutic efficacies of MDR in cancer. However, few effective MDR modulators have been identified so far. Curcumin has been reported to be an effective compound in the reversal of MDR in colorectal cancer cells. However, the mechanisms associated with the reversal effect of curcumin on MDR and its regulation of target factors in MDR cells remain to be fully elucidated...
December 2018: Molecular Medicine Reports
Qing Fang, Yuyin Zhu, Qilai Wang, Meijun Song, Guosheng Gao, Zhiming Zhou
Safe, affordable and efficacious agents are urgently required for cancer prevention. Sesamin, a lipid‑soluble lignan from sesame (Sesamum indicum) displays anticancer activities through an unknown mechanism. In the present study, the anticancer activity of sesamin via cyclooxygenase 2 (COX2) was investigated in lung cancer. Quantitative polymerase chain reaction was performed to determine the mRNA expression levels of COX2 in cells, while western blot analysis was used to determine its protein expression levels...
October 18, 2018: International Journal of Molecular Medicine
Yan Zhang, Jiangxia Fan, Yichao Fan, Lili Li, Xiaoqian He, Qin Xiang, Junhao Mu, Danfeng Zhou, Xuejuan Sun, Yucheng Yang, Guosheng Ren, Qian Tao, Tingxiu Xiang
BACKGROUND: Nasopharyngeal carcinoma (NPC) is prevalent in South China, including Hong Kong and Southeast Asia, constantly associated with Epstein-Barr virus (EBV) infection. Epigenetic etiology attributed to EBV plays a critical role in NPC pathogenesis. Through previous CpG methylome study, we identified Disheveled-associated binding antagonist of beta-catenin 2 (DACT2) as a methylated target in NPC. Although DACT2 was shown to regulate Wnt signaling in some carcinomas, its functions in NPC pathogenesis remain unclear...
February 27, 2018: Clinical Epigenetics
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