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tumor chemosensitivity assay

Keigo Yokoi, Hiroki Harada, Kazuko Yokota, Satoru Ishii, Toshimichi Tanaka, Nobuyuki Nishizawa, Masashi Shimazu, Ken Kojo, Hirohisa Miura, Takahiro Yamanashi, Takeo Sato, Takatoshi Nakamura, Masahiko Watanabe, Keishi Yamashita
BACKGROUND: Cysteine dioxygenase type 1 (CDO1) acts as a tumor suppressor gene, and its expression is regulated by promoter DNA methylation in human cancer. The metabolic product mediated by CDO1 enzyme increases mitochondrial membrane potential (MMP), putatively representing chemoresistance. The aim of this study is to investigate the functional relevance of CDO1 gene in colon cancer with chemotherapy. PATIENTS AND METHODS: We investigated 170 stage III colon cancer patients for CDO1 methylation by using quantitative methylation-specific polymerase chain reaction (PCR)...
October 11, 2018: Annals of Surgical Oncology
Chuiming Jia, Dejuan Kong, Yiwei Guo, Lianqiao Li, Lina Quan
Bortezomib (BTZ) is one of the most frequently used drugs in treatment of multiple myeloma (MM), but drug-resistance often occurs and limits its clinical efficacy. Annexin A1 (ANXA1) is upregulated in MM, and its knockdown enhances chemosensitivity in MM. However, whether ANXA1 inhibition can increase antitumor activity of BTZ in MM cells remains unknown. In the present study, Cell Counting Kit-8 (CCK-8) and colony formation assays showed that ANXA1 silencing combined with BTZ treatment led to a more significant inhibition of MM cell proliferation than each treatment alone...
October 3, 2018: Biochemical and Biophysical Research Communications
Chuchu Zhao, Ermei Lu, Xiaoli Hu, Huihui Cheng, Jian-An Zhang, Xueqiong Zhu
Objective: Our previous research has shown that the expression of S100 calcium-binding protein A9 (S100A9) in tumor cells was associated with neoadjuvant chemotherapy sensitivity in cervical squamous cell carcinoma. In the present study, we altered the expression of S100A9 through infecting lentivirus, investigated its effect on the chemosensitivity to cisplatin of cervical cancer cells and then made a primary exploration of the involved mechanism. Materials and methods: Lentivirus was employed to upregulate and downregulate S100A9 expression in SiHa cells...
2018: Cancer Management and Research
Xiaobing Liu, Xing Luo, Yuqi Wu, Ding Xia, Wei Chen, Zhenqiang Fang, Jianping Deng, Yaxing Hao, Xia Yang, Teng Zhang, Luqiang Zhou, Yingbing Wu, Qingqing Wang, Jie Xu, Xiaoyan Hu, Longkun Li
BACKGROUND/AIMS: Treatment options for metastatic castrate-resistant prostate cancer (mCRPC) are limited and typically centered on paclitaxel-based chemotherapy. In this study, we aimed to evaluate whether miR-34a attenuates chemoresistance to paclitaxel by regulating target genes associated with drug resistance. METHODS: We used data from The Cancer Genome Atlas to compare miR-34a expression levels in prostate cancer (PC) tissues with normal prostate tissues. The effects of miR-34a inhibition and overexpression on PC proliferation were evaluated in vitro via Cell Counting Kit-8 (CCK-8) proliferation, colony formation, apoptosis, and cell-cycle assays...
October 3, 2018: Cellular Physiology and Biochemistry
Huanling Li, Jie Liang, Feng Qin, Yunfang Zhai
MicroRNAs (miRNAs) are important regulators in tumorigenesis and progression of multiple human cancers, including ovarian cancer (OC). As a member of miRNAs family, miR-374b-5p has been reported to be a tumor suppressive gene in human cancers. In this study, the lower expression of miR-374b-5p was identified in OC tissues and cell liens using quantitative real time PCR (qRT-PCR). Forkhead box protein P1 (FOXP1) can act as an oncogene in human cancers. Mechanism experiments revealed that FOXP1 is a target of miR-374b-5p...
September 28, 2018: Biochemical and Biophysical Research Communications
Lei Yong, Yunlong Ma, Bin Zhu, Xiao Liu, Peng Wang, Chen Liang, Guanping He, Zhigang Zhao, Zhongjun Liu, Xiaoguang Liu
PURPOSE: Our previous studies have reported the antitumor effect of oleandrin on osteosarcoma; however, its chemosensitizing effect in osteosarcoma treatment is still unknown. Therefore, we explored the sensitizing effects of oleandrin to cisplatin in osteosarcoma and investigated the potential mechanisms. METHODS: After exposure to oleandrin and/or cisplatin, CCK-8 and colony formation assays, DAPI staining and flow cytometry were performed to detect cell proliferation and apoptosis in 143B, U-2OS and MG-63 osteosarcoma cells...
September 28, 2018: Cancer Chemotherapy and Pharmacology
Zhi-Hong Xu, Tie-Zhu Yao, Wei Liu
Ovarian cancer has gradually become one of the commonest gynecological tumor in the world. Although various therapies have been developed by researchers, the chemoresistance of ovarian cancer is still a huge challenge. MircroRNAs (miRNAs) have been widely studied due to their anti-oncogenic functions. MiR-378a-3p has been reported to sensitize breast cancer cells to chemotherapy. Here, we hypothesized that miR-378a-3p is a potential chemosensitizer in ovarian cancer. Firstly, miR-378a-3p was uncovered to down-regulated in ovarian cancer tissues and cell lines through using qRT-PCR analysis and northern blot analysis...
November 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jun Zhao, Jingjie Cao, Lurong Zhou, Yunyi Du, Xiaoling Zhang, Bo Yang, Yangjun Gao, Yu Wang, Ning Ma, Wei Yang
Colorectal cancer (CRC) is the most common malignant tumor type and has become resistant to 5-fluorouracil (5-FU) in recent decades, which is one of the most popular therapies. Recently, microRNA (miRNA or miR) has been investigated as a potential therapeutic strategy for CRC. However, there has been little investigation of the underlying mechanism of the association between expression of miRNA and chemosensitivity. The present study aimed to investigate the effect of miR-1260b inhibitor on CRC cells, and their chemosensitivity to 5-FU, by treating them with the miR-1260b inhibitor...
October 2018: Oncology Letters
Yuan-Yu Wu, Jun-Nan Jiang, Xue-Dong Fang, Fu-Jian Ji
In China, majority of the mortality in gastric cancer are associated with peritoneal metastasis. Since most gastric tumors are metastatic at initial diagnosis, the treatment of gastric cancer is limited to radical resection. Therefore, it is imperative to identify diagnostic and prognostic biomarkers. From 2014 to 2015, 20 patients were enrolled in the study. To search translationally upregulated genes in the context of epithelial to mesenchymal transition (EMT), polysome profiling was performed. The MTT, migration, and invasion assay were conducted to determine cell proliferation, migration, and invasion ability respectively...
2018: Frontiers in Physiology
Xiao Han, Jing-Jing Zhang, Zheng-Quan Han, Hai-Bin Zhang, Zi-An Wang
Platinum-based chemotherapy is currently a standard treatment strategy for patients with gastric cancer. Eventhough it has been widely shown that microRNAs (miRNAs) are involved in tumor development, whether miRNAs have a role in chemosensitivity of gastric cancer cells to platinum-based treatment remain largely undefined. In this study, a cisplatin-resistant gastric cancer cell line (SGC7901/DDP) with stable enhanced expression or knockdown of let-7b was generated. MTT and TUNEL assays were carried out to assess whether miR-let-7 is crucial for cell viability and apoptosis, respectively...
September 20, 2018: Cancer Gene Therapy
Wen-Jian Liu, Yi-Bo Yin, Jing-Yi Sun, Sai Feng, Jin-Kui Ma, Xiao-Yan Fu, Ya-Jun Hou, Ming-Feng Yang, Bao-Liang Sun, Cun-Dong Fan
Background: Temozolomide (TMZ)-based chemotherapy represents an effective way for treating human glioma. However, its clinical application is limited because of its side effects and resistance to standard chemotherapy. Hence, the search for novel chemosensitizers to augment their anticancer efficiency has attracted much attention. Natural borneol (NB) has been identified as a potential chemosensitizer in treating human cancers. However, the synergistic effect and mechanism of NB and TMZ in human glioma have not been investigated yet...
2018: OncoTargets and Therapy
Xin-Min Zheng, Chun-Wei Xu, Fei Wang
Osteosarcoma (OS) is one of the most common types of malignant bone tumor in adolescent. MicroRNAs (miRNAs) are widely studied regulatory molecules which play important roles in tumor development, differentiation, growth, invasion, chemosensitivity and cellular metabolism. Recently, miR-33b has been reported to act as a tumor suppressor in osteosarcoma. However, the detailed mechanism of miR-33b in regulating osteosarcoma cell proliferation remains unclear. In this study, we detected miR-33b was significantly downregulated in osteosarcoma tissues compared to their matched adjacent nontumor tissues...
August 30, 2018: Cellular and Molecular Biology
Changjin Chen, Ziyi Zhao, Shiyun Tang, Cuiwei Zhang
Rab-like protein 1 A (RBEL1A), which is a predominant isoform of RBEL1, has been identified to serve an important function in breast tumorigenesis and may be upregulated in breast tumor cells. RBEL1A may block the transcriptional activity of p53, which is important in the induction of cisplatin sensitivity. Previous studies supported the association between the induction of chemoresistance and the inhibition of p53 by RBEL1A. However, the response of RBEL1A to chemotreatment and its interaction with p53 remains to be investigated...
October 2018: Oncology Letters
Ya-Lan Zhou, Qiu-Mei Yao, Jiao Zhou, Yan Chang, Jin-Lan Li, Ya-Zhe Wang, Hong-Ping Wu, Yu-Hong Chen, Yan-Rong Liu, Xiao-Jun Huang, Guo-Rui Ruan
Background: Daunorubicin is a traditional chemotherapeutic agent that plays a pivotal role in leukemia therapy. However, the dose-related toxicity remains a considerable challenge. The apoptosis-regulating gene, PDCD5 , is downregulated in various tumors, including leukemias, and may provide a potential target for the diagnosis and treatment of leukemia. The purpose of this study was to construct a triple-regulated oncolytic adenovirus carrying a PDCD5 gene expression cassette (SG611-PDCD5) and explore the combined antitumor efficacy of SG611-PDCD5 in combination with low dose daunorubicin on leukemic cells...
2018: OncoTargets and Therapy
Wei Zhang, Hengfei Shi, Changmai Chen, Ke Ren, Yujun Xu, Xiaoyi Liu, Long He
BACKGROUND: Curcumin is a naturally occurring polyphenol which has been demonstrated to possess diverse biological activities. We previously reported that curcumin is a biologically active copper chelator with antitumor activity. Copper transporter 1 (CTR1) on the plasma membrane of eukaryotic cells mediates both copper as well as anticancer drug cisplatin uptake. PURPOSE: This study aims to investigate whether curcumin enhances cisplatin sensitivity of human non-small cell lung cancer (NSCLC) through influencing Cu-Sp1-CTR1 regulatory loop...
September 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Yong Li, Ping Gong, Ji-Xue Hou, Wei Huang, Xiao-Ping Ma, Yu-Li Wang, Jing Li, Xiao-Bin Cui, Na Li
Although aberrant expression of miR-34a, an essential tumor suppressor miRNA, has been frequently observed in colon cancer (CCa), whether miR-34a can regulate CCa progression by modulating other facets of this malignancy (such as multidrug resistance, MDR) remains unknown. Here, we report for the first time that miR-34a expression was significantly downregulated in clinical CCa samples from oxaliplatin-resistant patients and in experimentally established multidrug-resistant CCa cells. By using histoculture drug response assay, we further confirmed that clinical CCa samples with lower miR-34a expression appeared to be more resistant to chemotherapy...
2018: Journal of Immunology Research
Chunhui Lang, Miao Xu, Ziyi Zhao, Jinyao Chen, Lishi Zhang
MicroRNA-96 (miR-96) is transcriptionally associated with the induction of chemoresistance following chemotherapy by targeting to FOXO1 mRNA at one of two predicted binding sites in its 3'-untranslated region sequence. The upregulation of miR-96 is associated with a high risk of chemoresistance. Nevertheless, the mechanism by which miR-96 is upregulated remains largely undefined. In the present study, the gastric cancer SGC7901 cell line was treated with different doses of the chemotherapeutic agents cisplatin and doxorubicin...
September 2018: Oncology Letters
Qingyu Cui, Bixiao Wang, Kaifu Li, Haichen Sun, Tao Hai, Yan Zhang, Hua Kang
Chemotherapy is an important comprehensive treatment for breast cancer, which targets micro-environment of tumors as well as their characterisitcs. A previous microarray analysis revealed that matrix metalloproteinase (MMP)-1 was highly upregulated in carcinoma-associated fibroblasts (CAFs) prior to and following treatment with Taxotere under co-culture conditions. However, whether the chemotherapeutic effects of Taxotere were influenced by the changes in MMP-1 remained unclear. The purpose of the present study was to investigate the impact and mechanism of CAFs in regulating the efficacy of Taxotere on breast cancer cells...
September 2018: Oncology Letters
Yong Zhang, Sanxiu Huang, Yu Guo, Lingfeng Li
BACKGROUND: Dysregulation of miRNAs is critical for chemosensitivity to platinum-based agents in ovarian cancer (OC) which is the most aggressive gynecological cancer. However, the underlying mechanisms of miRNA-regulated platinum resistance in ovarian cancer remain unclear. In this study, we intended to investigate the effect of miR-1294 on platinum-resistant OC. METHODS: The expression of miR-1294 in OC tissues (n = 30) and cell lines was measured by qRT-PCR...
October 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Huixing Deng, Geng Qianqian, Ji Ting, Yang Aimin
microRNAs (miRNAs) have been implicated to play critical roles in non-small celllung cancer (NSCLC) progression and participate in the regulation of drug resistance during cancer chemotherapy. However, the underlying molecular mechanism of how miR-539 modulates the chemosensitivity to cisplatin (DDP) in NSCLC cells remains largely unknown. In this study, we found that miR-539 was significantly downregulated in DDP-resistant cell lines (A549/DDP and H1299/DDP). Overexpression of miR-539 enhanced the sensitivity of DDP-resistant NSCLC cells to DDP by suppressing cell proliferation, causing cell cycle arrest, inducing apoptosis, repressing invasion and migration...
October 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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