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https://www.readbyqxmd.com/read/28207794/protected-graft-copolymer-formulated-fibroblast-growth-factors-mitigate-the-lethality-of-partial-body-irradiation-injury
#1
Gerardo M Castillo, Akiko Nishimoto-Ashfield, Cynthia C Jones, Kasim K Kabirov, Alexander Zakharov, Alexander V Lyubimov
We evaluated the mitigating effects of fibroblast growth factor 4 and 7 (FGF4 and FGF7, respectively) in comparison with long acting protected graft copolymer (PGC)-formulated FGF4 and 7 (PF4 and PF7, respectively) administered to C57BL/6J mice a day after exposure to LD50/30 (15.7 Gy) partial body irradiation (PBI) which targeted the gastrointestinal (GI) system. The PGC that we developed increased the bioavailability of FGF4 and FGF7 by 5- and 250-fold compared to without PGC, respectively, and also sustained a 24 hr presence in the blood after a single subcutaneous administration...
2017: PloS One
https://www.readbyqxmd.com/read/28185978/long-term-serial-changes-in-platelet-activation-indices-following-sirolimus-eluting-and-bare-metal-stents-implantation-in-patients-with-stable-coronary-artery-disease
#2
Maria Marketou, George E Kochiadakis, Aikaterini Giaouzaki, Katerini Sfiridaki, Stelios Petousis, Fragiskos Maragoudakis, Konstantinos Roufas, Despoina Vougia, John Logakis, Gregory Chlouverakis, Panos E Vardas
BACKGROUND: Platelet activation is crucial in the development of stent thrombosis following percutaneous coronary intervention (PCI). We carried out a long-term assessment of multiple factors implicated in the thrombotic process and markers of platelet activation, after implantation of sirolimus-eluting stents (SES) in patients with stable coronary artery disease (CAD), and we compared the results with those after bare-metal stent (BMS) implantation. METHODS: Forty-seven consecutive patients, aged <70 years, with severe stenosis (>70% narrowing of the lumen) of one major epicardial coronary artery and stable CAD, underwent successful elective PCI and were randomly allocated to SES (n=25) or BMS (n=22)...
February 6, 2017: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/28167447/associations-between-ambient-wood-smoke-and-other-particulate-pollutants-and-biomarkers-of-systemic-inflammation-coagulation-and-thrombosis-in-cardiac-patients
#3
Daniel P Croft, Scott J Cameron, Craig N Morrell, Charles J Lowenstein, Frederick Ling, Wojciech Zareba, Philip K Hopke, Mark J Utell, Sally W Thurston, Kelly Thevenet-Morrison, Kristin A Evans, David Chalupa, David Q Rich
BACKGROUND: Increased particulate air pollution has been associated with both an increased risk of myocardial infarction (MI) and adverse changes in cardiac biomarkers. Up to 30% of ambient wintertime fine particles (PM2.5) in Rochester, NY are from wood burning. Our study examined associations between ambient levels of a marker of wood smoke (Delta-C) and other particulate air pollutants and biomarkers of inflammation, coagulation and thrombosis. METHODS: We measured blood concentrations of C-reactive protein (CRP), D-dimer, fibrinogen, P-selectin, platelet factor 4 (PF-4), von Willebrand factor (vWF), and myeloperoxidase (MPO) of 135 patients undergoing cardiac catheterization during the winters of 2011-2013...
February 3, 2017: Environmental Research
https://www.readbyqxmd.com/read/28118750/characterization-of-pf4-heparin-complexes-by-photon-correlation-spectroscopy-and-zeta-potential
#4
Sabrina Bertini, Jawed Fareed, Laura Madaschi, Giulia Risi, Giangiacomo Torri, Annamaria Naggi
Heparin-induced thrombocytopenia (HIT) is associated with antibodies to complexes between heparin and platelet factor 4 (PF4), a basic protein usually found in platelet alpha granules. Heparin-induced thrombocytopenia antibodies preferentially recognize macromolecular complexes formed between positively charged PF4 and polyanionic heparins over a narrow range of molar ratios. The aim of this work was to study the complexes that human PF4 forms with heparins from various species, such as porcine, bovine, and ovine; heparins from various organs, such as mucosa and lung; and different low-molecular-weight heparins (LMWHs) at several stoichiometric ratios to evaluate their sizes and charges by photo correlation spectroscopy and zeta potential measurements...
January 1, 2017: Clinical and Applied Thrombosis/hemostasis
https://www.readbyqxmd.com/read/28115521/platelet-factor-4-binds-to-vascular-proteoglycans-and-controls-both-growth-factor-activities-and-platelet-activation
#5
Megan S Lord, Bill Cheng, Brooke L Farrugia, Simon McCarthy, John M Whitelock
Platelet factor 4 (PF4) is produced by platelets with roles in both inflammation and wound healing. PF4 is stored in platelet α-granules bound to the glycosaminoglycan (GAG) chains of serglycin. This study revealed that platelet serglycin is decorated with chondroitin/dermatan sulfate (CS/DS) and that PF4 binds to these GAG chains. Additionally, PF4 had a higher affinity for endothelial-derived perlecan heparan sulfate (HS) chains than serglycin GAG chains. The binding of PF4 to perlecan was found to inhibit both FGF2 signaling and platelet activation...
January 23, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28052584/activated-platelet-derived-and-leukocyte-derived-circulating-microparticles-and-the-risk-of-thrombosis-in-heparin-induced-thrombocytopenia-a-role-for-pf4-bearing-microparticles
#6
Elena Campello, Claudia M Radu, Elena Duner, Anna M Lombardi, Luca Spiezia, Raffaele Bendo, Silvia Ferrari, Paolo Simioni, Fabrizio Fabris
BACKGROUND: Though the presence of platelets-derived microparticles (MPs) have previously been described in heparin-induced thrombocytopenia (HIT), the mechanism of thrombosis in HIT remains poorly understood. We aimed to assess the presence and origin of MPs in patients with HIT and their possible contribution to HIT with thrombosis (HITT). METHODS: Forty-five patients with HIT and 45 matched hospitalized patients with not confirmed HIT (HIT-negative) were enrolled...
January 3, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28008280/thicker-carotid-intima-media-thickness-and-increased-plasma-vegf-levels-suffered-by-post-acute-thrombotic-stroke-patients
#7
Yuyun Yueniwati, Ni Komang Darmiastini, Eko Arisetijono
BACKGROUND AND OBJECTIVES: Atherosclerosis causes reduction of the oxygen supply to structures in the far arterial wall, provoking the release of factors that drive angiogenesis of vasa vasorum, including VEGF. Other studies have revealed the inflammatory response in atherosclerosis and the role of platelet factor 4 (PF4) as an anti-angiogenic chemokine through the inhibition of VEGF. This cross-sectional study aims at measuring the effect of atherosclerosis assessed through carotid intima-media thickness (CIMT) against plasma VEGF levels in patients with post-acute thrombotic stroke...
2016: International Journal of General Medicine
https://www.readbyqxmd.com/read/27913490/heparin-induced-thrombocytopenia-research-and-clinical-updates
#8
Oluwatoyosi Onwuemene, Gowthami M Arepally
Heparin-induced thrombocytopenia (HIT) remains an important diagnosis to consider in hospitalized patients developing thrombocytopenia. HIT is an immune-mediated prothrombotic disorder caused by antibodies to platelet factor 4 (PF4) and heparin. Recent basic scientific studies have advanced our understanding of disease pathogenesis through studies of the PF4/heparin structure, immune mechanisms, and cellular basis of thrombosis. Clinical advances have also occurred in areas of HIT prevention, description of disease variants, and diagnostic strategies...
December 2, 2016: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/27829148/pf4-promotes-platelet-production-and-lung-cancer-growth
#9
Ferdinando Pucci, Steffen Rickelt, Andita P Newton, Christopher Garris, Ernesto Nunes, Charles Evavold, Christina Pfirschke, Camilla Engblom, Mari Mino-Kenudson, Richard O Hynes, Ralph Weissleder, Mikael J Pittet
Co-option of host components by solid tumors facilitates cancer progression and can occur in both local tumor microenvironments and remote locations. At present, the signals involved in long-distance communication remain insufficiently understood. Here, we identify platelet factor 4 (PF4, CXCL4) as an endocrine factor whose overexpression in tumors correlates with decreased overall patient survival. Furthermore, engineered PF4 over-production in a Kras-driven lung adenocarcinoma genetic mouse model expanded megakaryopoiesis in bone marrow, augmented platelet accumulation in lungs, and accelerated de novo adenocarcinogenesis...
November 8, 2016: Cell Reports
https://www.readbyqxmd.com/read/27812736/integrative-analysis-for-identification-of-shared-markers-from-various-functional-cells-tissues-for-rheumatoid-arthritis
#10
Wei Xia, Jian Wu, Fei-Yan Deng, Long-Fei Wu, Yong-Hong Zhang, Yu-Fan Guo, Shu-Feng Lei
Rheumatoid arthritis (RA) is a systemic autoimmune disease. So far, it is unclear whether there exist common RA-related genes shared in different tissues/cells. In this study, we conducted an integrative analysis on multiple datasets to identify potential shared genes that are significant in multiple tissues/cells for RA. Seven microarray gene expression datasets representing various RA-related tissues/cells were downloaded from the Gene Expression Omnibus (GEO). Statistical analyses, testing both marginal and joint effects, were conducted to identify significant genes shared in various samples...
November 3, 2016: Immunogenetics
https://www.readbyqxmd.com/read/27785869/probing-functional-heteromeric-chemokine-protein-protein-interactions-through-conformation-assisted-oxime-ligation
#11
Stijn M Agten, Rory R Koenen, Hans Ippel, Veit Eckardt, Philipp von Hundelshausen, Kevin H Mayo, Christian Weber, Tilman M Hackeng
Protein-protein interactions (PPIs) govern most processes in living cells. Current drug development strategies are aimed at disrupting or stabilizing PPIs, which require a thorough understanding of PPI mechanisms. Examples of such PPIs are heteromeric chemokine interactions that are potentially involved in pathological disorders such as cancer, atherosclerosis, and HIV. It remains unclear whether this functional modulation is mediated by heterodimer formation or by the additive effects of mixed chemokines on their respective receptors...
November 21, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27772839/pf4-induced-apoptosis-in-breast-cancer-in-vivo-study-the-role-of-the-caspases-family
#12
Tengku Ahmad Damitri Al Astani Tengku Din, Azman Seeni, Muhamad Saiful Bahri Yusoff, Shaharum Shamsuddin, Hasnan Jaafar
No abstract text is available yet for this article.
February 2016: Pathology
https://www.readbyqxmd.com/read/27734602/recurrent-heparin-induced-thrombocytopenia-due-to-heparin-rinsing-before-priming-the-machine-in-a-hemodialysis-patient-a-case-report
#13
Jin Han Lim, Kyung Pyo Kang, Sik Lee, Sung Kwang Park, Won Kim
Heparin has remained the most commonly used anticoagulant for patients undergoing hemodialysis. It is usually safe to use but can have severe adverse effects in some cases. Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of exposure to heparin. It results from an autoantibody directed against endogenous platelet factor 4 (PF4) in complex with heparin, which activates platelets and can cause catastrophic arterial and venous thromboses. Here, we present the case of an 80-year-old woman with a recent diagnosis of chronic renal failure who developed acute HIT (platelet count nadir, 15 × 10(9) /L) on day 7 of hemodialysis performed with routine heparin anticoagulation, who despite subsequent heparin-free hemodialysis (with argatroban and warfarin) developed recurrent HIT (complicated by acute cerebral infarction) on day 11 that we attributed to "rinsing" of the circuit with heparin-containing saline (3,000 units of unfractionated heparin, with subsequent saline washing) performed pre-dialysis as per routine...
October 13, 2016: Hemodialysis International
https://www.readbyqxmd.com/read/27720587/composition-of-growth-factors-and-cytokines-in-lysates-obtained-from-fresh-versus-stored-pathogen-inactivated-platelet-units
#14
Felix Sellberg, Erik Berglund, Martin Ronaghi, Gabriel Strandberg, Helena Löf, Pehr Sommar, Norbert Lubenow, Folke Knutson, David Berglund
BACKGROUND: Platelet lysate is a readily available source of growth factors, and other mediators, which has been used in a variety of clinical applications. However, the product remains poorly standardized and the present investigation evaluates the composition of platelet lysate obtained from either fresh or stored pathogen-inactivated platelet units. MATERIALS AND METHODS: Platelet pooled units (n = 10) were obtained from healthy blood donors and tested according to standard procedures...
December 2016: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/27716443/a-discrete-cluster-of-urinary-biomarkers-discriminates-between-active-systemic-lupus-erythematosus-patients-with-and-without-glomerulonephritis
#15
Carolina Landolt-Marticorena, Stephenie D Prokopec, Stacey Morrison, Babak Noamani, Dennisse Bonilla, Heather Reich, James Scholey, Carmen Avila-Casado, Paul R Fortin, Paul C Boutros, Joan Wither
BACKGROUND: Management of lupus nephritis (LN) would be greatly aided by the discovery of biomarkers that accurately reflect changes in disease activity. Here, we used a proteomics approach to identify potential urinary biomarkers associated with LN. METHODS: Urine was obtained from 60 LN patients with paired renal biopsies, 25 active non-LN SLE patients, and 24 healthy controls. Using Luminex, 128 analytes were quantified and normalized to urinary creatinine levels...
October 4, 2016: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/27714919/the-association-of-anti-platelet-factor-4-heparin-antibodies-with-early-and-delayed-thromboembolism-after-cardiac-surgery
#16
I J Welsby, E F Krakow, J A Heit, E C Williams, G M Arepally, S Bar-Yosef, D F Kong, S Martinelli, I Dhakal, W W Liu, J Krischer, T L Ortel
: Essentials We evaluated antibody status, thromboembolism and survival after cardiac surgery. Positive antibody tests are common - over 50% are seropositive at 30 days. Seropositivity did not increase thromboembolism or impair survival after cardiac surgery. Results show heparin induced thrombocytopenia antibody screening after surgery is not warranted. SUMMARY: Background Heparin-induced thrombocytopenia (HIT) is a prothrombotic response to heparin therapy with platelet-activating, anti-platelet factor 4 (PF4)/heparin antibodies leading to thrombocytopenia associated with thromboembolism...
January 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/27658429/biophysical-tools-to-assess-the-interaction-of-pf4-with-polyanions
#17
Mihaela Delcea, Andreas Greinacher
The antigen in heparin-induced thrombocytopenia (HIT) is expressed on platelet factor 4 (PF4) when PF4 complexes with polyanions. In recent years, biophysical tools (e. g. circular dichroism spectroscopy, atomic force microscopy, isothermal titration calorimetry, x-ray crystallography, electron microscopy) have gained an important role to complement immunological and functional assays for better understanding the interaction of heparin with PF4. This allowed identification of those features that make PF4 immunogenic (e...
October 28, 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27631372/the-role-of-cytokine-pf4-in-the-antiviral-immune-response-of-shrimp
#18
Yulei Chen, Jiao Cao, Xiaobo Zhang
During viral infection in vertebrates, cytokines play important roles in the host defense against the virus. However, the function of cytokines in invertebrates has not been well characterized. In this study, shrimp cytokines involved in viral infection were screened using a cytokine antibody microarray. The results showed that three cytokines, the Fas receptor (Fas), platelet factor 4 (PF4) and interleukin-22 (IL-22), were significantly upregulated in the white spot syndrome virus (WSSV)-challenged shrimp, suggesting that these cytokines played positive regulatory roles in the immune response of shrimp against the virus...
2016: PloS One
https://www.readbyqxmd.com/read/27609736/utilization-of-4t-score-to-determine-the-pretest-probability-of-heparin-induced-thrombocytopenia-in-a-community-hospital-in-upstate-new-york
#19
Yazan Samhouri, Mohammad Telfah, Ruth Kouides, Timothy Woodlock
BACKGROUND: Thrombocytopenia is common in hospitalized patients. Heparin-induced thrombocytopenia (HIT) is a life-threatening condition which can lead to extensive thrombosis. Diagnosis of HIT relies on clinical suspicion determined by 4T score and immunoassays through testing for anti-PF4/heparin antibodies. Clinical practice guidelines published by the American Society of Hematology in 2013 recommended use of the 4T score before ordering the immunoassays as a measure of pretest probability...
2016: Journal of Community Hospital Internal Medicine Perspectives
https://www.readbyqxmd.com/read/27597121/acute-intraoperative-heparin-induced-thrombocytopenia-hit-and-thrombosis-during-coronary-artery-bypass-grafting-two-case-reports-providing-evidence-for-the-role-of-preoperative-lmwh-in-triggering-sensitization
#20
M Khoury, A Pitsis, H Poumpouridou-Kioura, G Soufla, C Kanthou, N Matoula, A Angelidis, E Melissari
INTRODUCTION: Systemic anticoagulation is necessary during cardiac surgery. To date, the only well established anticoagulation protocol involves the use of heparin. However, heparin can cause heparin-induced thrombocytopenia (HIT) a potentially life threatening immune-mediated thromboembolic syndrome. Until now, devastating consequences of HIT syndrome in patients undergoing heart surgery have been described, but only postoperatively. Here we report the development of HIT syndrome during cardiac revascularization by intra-operative heparin administration in two patients previously exposed to LMWH...
October 2016: Thrombosis Research
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