keyword
MENU ▼
Read by QxMD icon Read
search

Pf4

keyword
https://www.readbyqxmd.com/read/29146335/antioxidant-activity-and-sensory-characteristics-of-maillard-reaction-products-derived-from-different-peptide-fractions-of-soybean-meal-hydrolysate
#1
Min Yu, Shudong He, Mingming Tang, Zuoyong Zhang, Yongsheng Zhu, Hanju Sun
Four peptide fractions PF1 (>5;kDa), PF2 (3-5;kDa), PF3 (1-3;kDa), PF4 (<1;kDa) were isolated from soybean hydrolysate using the ultrafiltration method. Then, d-xylose and l-cysteine were reacted with specific peptide solution at 120;°C for 2;h, and the molecular weight distribution (MWD), pH, colour, browning intensity, DPPH radical-scavenging activity, free amino acids and sensory characteristics of corresponding Maillard reaction products (MRPF1, MRPF2, MRPF3 and MRPF4) were evaluated, respectively...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29107969/assessing-single-stranded-oligonucleotide-drug-induced-effects-in-vitro-reveals-key-risk-factors-for-thrombocytopenia
#2
Sabine Sewing, Adrian B Roth, Michael Winter, Andreas Dieckmann, Cristina Bertinetti-Lapatki, Yann Tessier, Claudia McGinnis, Sylwia Huber, Erich Koller, Corinne Ploix, John C Reed, Thomas Singer, Andreas Rothfuss
Single-stranded oligonucleotides (ON) comprise a promising therapeutic platform that enables selective modulation of currently undruggable targets. The development of novel ON drug candidates has demonstrated excellent efficacy, but in certain cases also some safety liabilities were reported. Among them are events of thrombocytopenia, which have recently been evident in late stage trials with ON drugs. The underlying mechanisms are poorly understood and the risk for ON candidates causing such events cannot be sufficiently assessed pre-clinically...
2017: PloS One
https://www.readbyqxmd.com/read/29104956/deletion-of-the-arp2-3-complex-in-megakaryocytes-leads-to-microthrombocytopenia-in-mice
#3
David S Paul, Caterina Casari, Congying Wu, Raymond Piatt, Swetha Pasala, Robert A Campbell, Kathryn O Poe, Dorsaf Ghalloussi, Robert H Lee, Jeremy D Rotty, Brian C Cooley, Kellie R Machlus, Joseph E Italiano, Andrew S Weyrich, James E Bear, Wolfgang Bergmeier
Actin reorganization regulates key processes in platelet activation. Here we examined the role of the Arp2/3 complex, an essential component in actin filament branching, in platelet function. The Arpc2 gene, encoding the p34 subunit of the Arp2/3 complex, was deleted in the megakaryocyte lineage (Arpc2(fl/fl)PF4-Cre). Deletion of the Arp2/3 complex resulted in marked microthrombocytopenia in mice, caused by premature platelet release into the bone marrow compartment and impaired platelet survival in circulation...
August 8, 2017: Blood Advances
https://www.readbyqxmd.com/read/29098740/does-non-contact-low-frequency-ultrasound-nlfu-therapy-contribute-to-wound-healing-at-the-molecular-level
#4
Cornelia Wiegand, Kyle Bittenger, Robert D Galiano, Vickie R Driver, Gary W Gibbons
Non-contact low frequency ultrasound (NLFU) is used to treat various types of chronic wounds including venous, diabetic and pressure ulcers. The objective for this sub study of the IN BALANCE RCT VLU trial was to characterize and compare the NLFU treatment group and patients receiving standard of care (SOC) with respect to the effect of the assigned study treatment on content/quantity of inflammatory cytokines and fibrinogen as well as bacteria. Higher mean wound area reduction was observed in the NLFU treatment group (67...
November 2, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29048130/beneficial-effect-of-exogenous-platelet-factor-4-for-detecting-pathogenic-heparin-induced-thrombocytopenia-antibodies
#5
Caroline Vayne, Eve-Anne Guery, Claire Kizlik-Masson, Jérôme Rollin, Anne Bauters, Yves Gruel, Claire Pouplard
The laboratory diagnosis of heparin-induced thrombocytopenia (HIT) is based on an enzyme immunoassay combined with a functional test, and serotonin release assay (SRA) is the gold standard for detecting activating HIT antibodies. However, a recent atypical history of HIT prompted us to evaluate whether addition of platelet factor 4 (PF4) during SRA could improve its ability to detect pathogenic HIT antibodies. Using 5B9, a monoclonal antibody to PF4/H with a human Fc fragment, we first defined the optimal PF4 concentration for detecting low amounts of platelet-activating IgG with SRA...
October 19, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28991552/a-platelet-factor-4-dependent-platelet-activation-assay-facilitates-early-detection-of-pathogenic-heparin-induced-thrombocytopenia-antibodies
#6
Curtis G Jones, Shannon M Pechauer, Brian R Curtis, Daniel W Bougie, Mehraboon S Irani, Binod Dhakal, Brenda Pierce, Richard H Aster, Anand Padmanabhan
Heparin-induced thrombocytopenia (HIT) is a dangerous complication of heparin therapy. HIT diagnosis is established by recognizing thrombocytopenia and/or thrombosis in an affected patient and from the results of serological tests such as the platelet factor 4 (PF4)/heparin immunoassay (PF4 ELISA) and serotonin release assay (SRA). Recent studies suggest that HIT antibodies activate platelets by recognizing PF4 in a complex with platelet glycosaminoglycans (and/or polyphosphates) and that an assay based on this principle, the PF4-dependent P-selectin expression assay (PEA), may be even more accurate than the SRA for HIT diagnosis...
October 2017: Chest
https://www.readbyqxmd.com/read/28990414/biomarkers-of-endothelial-renal-and-platelet-dysfunction-in-stage-5-chronic-kidney-disease-hemodialysis-patients-with-heart-failure
#7
Ryan McMillan, Leonidas Skiadopoulos, Debra Hoppensteadt, Nil Guler, Vinod Bansal, Ravipresenna Parasuraman, Jawed Fareed
The aim of this study was to determine the role of endothelial, renal, and inflammatory biomarkers in the pathogenesis of heart failure (HF) in patients with stage 5 chronic kidney disease (CKD5) undergoing maintenance hemodialysis (HD). Plasma levels of biomarkers-kidney injury molecule 1 (KIM-1), N-terminal pro brain natriuretic peptide (NT-proBNP), glycated hemoglobin, neutrophil gelatinase-associated lipocalin, interleukin-18,platelet-derived growth factor, platelet factor 4 (PF4), 25-OH vitamin D, parathyroid hormone (PTH), endothelin, and endocan-were measured in CKD5-HD patients at the Loyola University Ambulatory Dialysis facility...
January 1, 2017: Clinical and Applied Thrombosis/hemostasis
https://www.readbyqxmd.com/read/28982395/pseudomonas-phage-inhibition-of-candida-albicans
#8
Hasan Nazik, Lydia-Marie Joubert, Patrick R Secor, Johanna M Sweere, Paul L Bollyky, Gabriele Sass, Lynette Cegelski, David A Stevens
Pseudomonas aeruginosa (Pa) and Candida albicans (Ca) are major bacterial and fungal pathogens in immunocompromised hosts, and notably in the airways of cystic fibrosis patients. The bacteriophages of Pa physically alter biofilms, and were recently shown to inhibit the biofilms of Aspergillus fumigatus. To understand the range of this viral-fungal interaction, we studied Pa phages Pf4 and Pf1, and their interactions with Ca biofilm formation and preformed Ca biofilm. Both forms of Ca biofilm development, as well as planktonic Ca growth, were inhibited by either phage...
October 6, 2017: Microbiology
https://www.readbyqxmd.com/read/28978870/anthocyanin-cyanidin-3-glucoside-attenuates-platelet-granule-release-in-mice-fed-high-fat-diets
#9
Fang-Hang Zhou, Xiu-Juan Deng, Yan-Qiu Chen, Fu-Li Ya, Xian-Dan Zhang, Fenglin Song, Dan Li, Yan Yang
Platelet granule release is considered an important target for preventing and treating cardiovascular diseases (CVDs). Cyanidin-3-glucoside (Cy-3-g) is a predominant bioactive anthocyanin compound in many edible plants and has been reported to be protective against CVDs by attenuating platelet dysfunction. However, direct evidence of the action of Cy-3-g on platelet granule secretion in purified platelets from in vivo assays is still poor. In the present study, we demonstrated that dietary supplementation of purified Cy-3-g reduces serum lipid levels and facilitates down-regulation of the platelet granule release of substances such as P-selectin, CD40L, 5-HT, RANTES and TGF-β1 in gel-filtered platelets, in addition to attenuating serum PF4 and β-TG levels in mice fed high-fat diets...
2017: Journal of Nutritional Science and Vitaminology
https://www.readbyqxmd.com/read/28968537/pf4-hit-antibody-kko-complexes-activate-broad-innate-immune-and-inflammatory-responses
#10
Lydia A Haile, Roshni Rao, Swamy K Polumuri, Gowthami M Arepally, David A Keire, Daniela Verthelyi, Cynthia D Sommers
INTRODUCTION: Heparin-induced thrombocytopenia (HIT) is an immune-mediated complication of heparin anticoagulation therapy resulting in thrombocytopenia frequently accompanied by thrombosis. Current evidence suggests that HIT is associated with antibodies developed in response to multi-molecular complexes formed by platelet factor 4 (PF4) bound to heparin or cell surface glycosaminoglycans. These antibody complexes activate platelets and monocytes typically through FcγRIIA receptors increasing the production of PF4, inflammatory mediators, tissue factor and thrombin...
September 21, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28947991/plasma-protein-profiling-in-patients-undergoing-coronary-artery-bypass-grafting-surgery-and-clinical-significance
#11
Zhi-Peng Guo, Hai-Tao Hou, Rui Jing, Zhen-Guo Song, Xiao-Cheng Liu, Guo-Wei He
This study was designed to identify the protein profiling in patients with triple vessel coronary artery disease (CAD) undergoing CABG, in order to detect CAD-related differential proteins in these patients. CABG patients with triple vessel disease with/without left main stenosis (n =160) were compared to normal coronary angiographic subjects (n =160). Plasma samples of 20 males and 20 females in each group were analyzed with iTRAQ technique. ELISA test was used to test the chosen proteins from iTRAQ results in plasma samples from a new cohort of the CABG group (n=120, male/femal=61/59) and control (n =120, male/female=60/60)...
September 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28922541/effects-of-collagen-peptides-intake-on-skin-ageing-and-platelet-release-in-chronologically-aged-mice-revealed-by-cytokine-array-analysis
#12
Hongdong Song, Ling Zhang, Yongkang Luo, Siqi Zhang, Bo Li
Action mechanisms underlying various biological activities of collagen peptides (CPs) remained to be elucidated. Cytokines may play an important role in mediating these health benefits of CPs. This study aimed to systemically examine the cytokines in skin and blood regulated by CPs intake. Thirteen-month-old female Kunming mice were administered with CPs for 2 months (0 or 400 mg/kg bodyweight/day). The cytokines in skin and plasma were analysed using a 53-cytokine array and corresponding ELISA kits. In skin, CPs intake significantly down-regulated placenta growth factor (PIGF-2), insulin-like growth factor (IGF)-binding protein (IGFBP) -2 and IGFBP-3, and up-regulated platelet factor 4 (PF4), serpin E1 and transforming growth factor (TGF)-β1 ...
September 18, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28914425/platelet-factor-4-increases-bone-marrow-b-cell-development-and-differentiation
#13
David J Field, Angela A Aggrey-Amable, Sara K Blick, Sara K Ture, Andrew Johanson, Scott J Cameron, Sukanya Roy, Craig N Morrell
Platelet factor 4 (PF4) is a megakaryocyte-/platelet-derived chemokine with diverse functions as a regulator of vascular and immune biology. PF4 has a central role in vessel injury responses, innate immune cell responses, and T-helper cell differentiation. We have now discovered that PF4 has a direct role in B cell differentiation in the bone marrow. Mice lacking PF4 (PF4(-/-) mice) had fewer developing B cells in the bone marrow beginning after the pre-pro-B cell stage of differentiation. In vitro, PF4 increased the differentiation of hematopoietic progenitors to B cell lineage cells, indicating that PF4 has a direct effect on B cell differentiation...
October 2017: Immunologic Research
https://www.readbyqxmd.com/read/28903994/the-level-of-heparin-induced-antibodies-in-correlation-with-the-result-of-the-flow-cytometric-functional-assay-in-the-patients-with-suspected-hit
#14
Elvira Maličev, Marjeta Maček Kvanka, Polona Klemenc, Primož Rožman
Heparin can induce the formation of antibodies against a heparin complex with a platelet factor 4 (PF4), leading to platelet activation and the development of heparin-induced thrombocytopaenia (HIT). Because screening ELISA does not discriminate between platelet activating and non-activating anti-heparin/PF4 antibodies, each positive result is confirmed by an additional functional assay. We analysed 1004 sera of patients with suspected HIT. Optical density (OD) values of ELISA-positive results were correlated with the risk for a positive result with our functional flow cytometric assay...
September 13, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28903944/a-dual-role-for-the-class-iii-pi3k-vps34-in-platelet-production-and-thrombus-growth
#15
Colin Valet, Marie Levade, Gaëtan Chicanne, Benoit Bilanges, Cendrine Cabou, Julien Viaud, Marie-Pierre Gratacap, Frédérique Gaits-Iacovoni, Bart Vanhaesebroeck, Bernard Payrastre, Sonia Severin
To uncover the role of Vps34, the sole class III phosphoinositide 3-kinase, in megakaryocytes (MKs) and platelets, we created a mouse model with Vps34 deletion in the MK/platelet lineage (Pf4-Cre/Vps34(lox/lox)). Deletion of Vps34 in MKs led to the loss of its regulator protein Vps15, and was associated with microthrombocytopenia and platelet granule abnormalities. While Vps34 deficiency did not impact on MK polyploidisation or proplatelet formation, it dampened MK granule biogenesis and directional migration towards an SDF1α gradient, leading to ectopic platelet release within the bone marrow...
September 13, 2017: Blood
https://www.readbyqxmd.com/read/28899579/cxcl4-induced-macrophages-in-human-atherosclerosis
#16
Gabriele Domschke, Christian A Gleissner
Atherosclerosis is considered an inflammatory disease of the arterial wall. Monocytes and monocyte-derived cells (most often termed macrophages) play an essential role in the formation of atherosclerotic lesions, as they take up lipids leading to subsequent foam cell formation accompanied by release of pro-inflammatory cytokines. Similarly, platelets have been discovered to represent an important cell type mediating inflammatory and immune processes in atherogenesis, mainly by secreting chemokines, which are stored in the platelets' alpha granules, upon platelet activation...
September 9, 2017: Cytokine
https://www.readbyqxmd.com/read/28867175/thrombo-hemorrhagic-liability-in-children-with-congenital-heart-diseases
#17
REVIEW
Shebl Said Shebl, Walid Ahmed Naguib El-Shehaby, Amira Hamed Darwish, Yasmin Shebl Said, Nabeh Helal Elfadaly, Eman Amer
BACKGROUND: The precise mechanisms of the increased incidence of hemostatic abnormalities in congenital heart disease (CHD) have not been determined. The aim of the study was to evaluate some indicators of activation of platelets and vascular endothelial cells in patients with CHD, evaluation of bleeding liability of these patients, and correlation with the clinical presentation of these patients. METHODS: This work was carried out on 20 patients with cyanotic congenital heart disease (CCHD), 20 patients with cyanotic congenital heart disease (ACHD), and 20 healthy children who served as the control group, aged between 1 and 10years...
August 26, 2017: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/28856946/spontaneous-hit-syndrome-post-knee-replacement-surgery-with-delayed-recovery-of-thrombocytopenia-a-case-report-and-literature-review
#18
Dilli Ram Poudel, Sushil Ghimire, Rashmi Dhital, Daniel A Forman, Theodore E Warkentin
Recently published reports have established a heparin-induced thrombocytopenia (HIT)-mimicking thromboembolic disorder without proximate heparin exposure, called spontaneous HIT syndrome. Although the pathophysiology remains unclear, anti-platelet factor 4 (PF4)/heparin antibodies possibly triggered by exposure to knee cartilage glycosaminoglycans or other non-heparin polyanions found on bacterial surfaces and nucleic acids have been postulated. We present a 53-year-old female receiving antithrombotic prophylaxis with aspirin following right total knee replacement surgery (without perioperative or any previous lifetime heparin exposure) who acutely presented with high-risk pulmonary embolism (PE) and right great saphenous vein thrombophlebitis on postoperative day (POD) 14; her platelet count at presentation was 13 × 10(9)/L...
September 2017: Platelets
https://www.readbyqxmd.com/read/28846826/autoimmune-heparin-induced-thrombocytopenia
#19
REVIEW
A Greinacher, K Selleng, T E Warkentin
Autoimmune heparin-induced thrombocytopenia (aHIT) indicates the presence in patients of anti-platelet factor 4 (PF4)-polyanion antibodies that are able to activate platelets strongly even in the absence of heparin (heparin-independent platelet activation). Nevertheless, as seen with serum obtained from patients with otherwise typical heparin-induced thrombocytopenia (HIT), serum-induced platelet activation is inhibited at high heparin concentrations (10-100 IU mL(-1) heparin). Furthermore, upon serial dilution, aHIT serum will usually show heparin-dependent platelet activation...
August 28, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28832327/b-cells-pf4-heparin-complexes-and-complement
#20
Theodore E Warkentin
No abstract text is available yet for this article.
October 6, 2016: Blood
keyword
keyword
15880
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"