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Wound swab culture

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https://www.readbyqxmd.com/read/30082109/incidence-and-risk-factors-associated-with-infection-after-intramedullary-nailing-of-femoral-and-tibial-diaphyseal-fractures-prospective-study
#1
Priscila R Oliveira, Marcos C Leonhardt, Vladimir C Carvalho, Kodi E Kojima, Jorge S Silva, Flavia Rossi, Ana Lucia L Lima
BACKGROUND: Diaphyseal fractures of femur and tibia are prominent due to its high incidence and high economic and social impact. Intramedullary nailing (IN) is the surgical procedure of choice. Surgical site infection (SSI) related to this procedure is considered a difficult to treat complication. AIMS: Determine the incidence of SSI after IN of femoral and tibial diaphyseal fractures and evaluate possible risk factors. METHODS: Prospective observational cohort study...
July 27, 2018: Injury
https://www.readbyqxmd.com/read/30016139/classification-microbiology-and-treatment-of-diabetic-foot-infections
#2
Reza Ghotaslou, Mohammad Yousef Memar, Naser Alizadeh
Diabetic foot ulcers (DFUs) are a common complication of type-1 and type-2 diabetes. About 10-15% of patients with diabetes develop foot ulcers. A validated foot ulcer classification system that will support the development of treatment strategis is necessary for clinicians managing DFUs. More than 10 classification systems have been described by researchers. Another important aspect of the management of DFUs is the proper identification of causative pathogens that trigger infections. While conventional diagnostic methods, such as swabs, cultures and biopsies are more widely used, novel molecular techniques have been exploring bacterial identification and quantification...
July 2, 2018: Journal of Wound Care
https://www.readbyqxmd.com/read/30006029/causative-agents-and-antimicrobial-resistance-patterns-of-human-skin-and-soft-tissue-infections-in-bagamoyo-tanzania
#3
Theckla Kazimoto, Salim Abdulla, Leah Bategereza, Omar Juma, Francis Mhimbira, Maja Weisser, Jürg Utzinger, Lutz von Müller, Sören L Becker
Few epidemiological studies have been carried out to assess the aetiology and antimicrobial susceptibility patterns of pathogens giving rise to skin and soft tissue infections (SSTIs) in sub-Saharan Africa. In the present study from six healthcare facilities in Bagamoyo, Tanzania, wound swabs from outpatients with SSTIs were analysed by a suite of methods, including microbiological culture techniques, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and resistance testing. Among 185 patients with SSTIs, 179 (96...
October 2018: Acta Tropica
https://www.readbyqxmd.com/read/29949241/the-spectrum-of-intraoral-bacteria-seen-in-patients-with-cleft-palates-in-an-african-setting
#4
Shaal Ramdial, Anil Madaree
Dehiscence or palatal fistula formation following palatoplasty is a complication that has grave consequences for the patient that include tissue loss, emotional distress to the parents and patient, and further medical costs. Palatal dehiscence or fistula formation is multifactorial following surgery-tension of wound closure, poor patient adherence to postoperative orders and wound infection are the most common causes for this. Oral colonization with pathogenic organisms could play a role in wound healing complications...
June 27, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29847418/a-prospective-comparative-study-in-skin-antiseptic-solutions-for-posterior-spine-surgeries-chlorhexidine-gluconate-ethanol-versus-povidone-iodine
#5
Toshitaka Yoshii, Takashi Hirai, Tsuyoshi Yamada, Kenichiro Sakai, Shuta Ushio, Satoru Egawa, Masato Yuasa, Tsuyoshi Kato, Hiroyuki Inose, Shigenori Kawabata, Atsushi Okawa
STUDY DESIGN: This is a prospective comparative study. OBJECTIVE: We evaluated the efficacy of 2 standard antiseptic solutions, chlorhexidine-gluconate (CHG) and povidone-iodine (PD-I), in eliminating bacterial pathogens from surgical sites in posterior spine surgeries. SUMMARY OF BACKGROUND DATA: Previous studies have shown that CHG is more effective for skin antisepsis than PD-I in joint surgeries. However, few studies have investigated the preoperative use of antiseptic solutions in spine surgery...
August 2018: Clinical Spine Surgery
https://www.readbyqxmd.com/read/29784040/antimicrobial-resistance-profile-of-staphylococcus-aureus-isolated-from-patients-with-infection-at-tikur-anbessa-specialized-hospital-addis-ababa-ethiopia
#6
Sileshi Tadesse, Haile Alemayehu, Admasu Tenna, Getachew Tadesse, Tefaye Sisay Tessema, Workineh Shibeshi, Tadesse Eguale
BACKGROUND: Staphylococcus aureus is one of the major pathogens of public health importance responsible for various forms of infection. Development of resistance to commonly used antimicrobials limited treatment options against infections due to this pathogen. Antimicrobial resistance profile of Staphylococcus aureus isolated from patients with surgical site infection and ear infection and corresponding nasal swab was investigated in Tikur Anbessa Specialized Hospital (TASH), Addis Ababa, Ethiopia...
May 21, 2018: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/29774178/effects-of-local-application-of-nano-silver-on-osteomyelitis-and-soft-tissue-infections-an-experimental-study-in-rats
#7
Bahattin Kemah, Gökçer Uzer, Yalçın Turhan, Burak Özturan, Bülent Kılıç, Bilge Sümbül Gültepe, Ayşe Bahar Ceyran, Selim Ertürk, Burak Aksoylu, Özlem Şenaydın, Korhan Özkan
Purpose : Bone and soft tissue infections are among the least desired complications after orthopaedic surgery. This study analysed the in vivo effects of the local application of nano-silver particles (AgNPs) [1nm = 1 billionth of a meter] in soft tissue infections. Materials-Method : An experimental osteomyelitis model was formed by inoculating both tibias of 24 rats with methicillin-resistant Staphylococcus aureus . The rats were followed without treatment for 21 days. Blood samples and tibial x-rays at day 21 confirmed the development of infection...
2018: Journal of Bone and Joint Infection
https://www.readbyqxmd.com/read/29733329/effectiveness-of-a-chlorhexidine-dressing-on-silver-coated-external-ventricular-drain-associated-colonization-infection-a-prospective-single-blinded-randomized-controlled-clinical-trial-evdai-study
#8
Michel Roethlisberger, Giusi Moffa, Urs Fisch, Benedikt Wiggli, Stephan Schoen, Christopher Kelly, Severina Leu, Davide Croci, Daniel W Zumofen, Nadine Cueni, Danica Nogarth, Marianne Schulz, Heiner C Bucher, Maja Weisser-Rohacek, Morten-Goetz Wasner, Andreas F Widmer, Luigi Mariani
Background: Observational studies have shown that dressings containing chlorhexidine gluconate (CHX) lower the incidence external ventricular drain (EVD) associated infections (EVDAI). This prospective, randomized controlled trial (RCT) studies the efficacy of CHX-containing dressings in reducing bacterial colonization, which represents an important risk factor for infectious complications. Methods: In this single-site, prospective, double-blinded RCT, patients aged ≥18 undergoing emergency EVD placement were randomly given either a CHX- or an otherwise identical control dressing at the skin exit wound of the EVD...
May 4, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29727894/-comparison-of-the-antibacterial-efficacy-of-polyhexanide-cold-atmospheric-argon-plasma-and-saline-in-the-treatment-of-canine-bite-wounds
#9
Selina Winter, Mirja Christine Nolff, Sven Reese, Andrea Meyer-Lindenberg
OBJECTIVE: To evaluate the bacterial contamination rate and to compare the efficacy of polyhexanide, cold argon plasma and saline at reducing bacterial bio-burden in dog bite wounds. MATERIAL AND METHODS: Dogs with bite-wound injuries were included when surgical debridement was pursued with subsequent treatment using either polyhexanide-biguanide lavage (A), cold argon plasma treatment (B) or saline lavage (C). Culture swabs were taken after debridement as well as after lavageor argon treatment...
April 2018: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
https://www.readbyqxmd.com/read/29698074/use-of-a-compounded-poloxamer-407-antibiotic-topical-therapy-as-part-of-the-successful-management-of-chronic-ulcerative-dermatitis-in-a-congo-african-grey-parrot-psittacus-erithacus
#10
Anthony A Pilny
A 23-year-old, 425-g male African grey parrot ( Psittacus erithacus) was evaluated for chronic ulcerative dermatitis of the axillary regions under both wings. Initial swab cultures of the sites had revealed a coagulase-positive methicillin-resistant Staphylococcus aureas (MRSA) with marked antibiotic resistance. A second swab culture obtained 8 weeks after the initial culture showed heavy growth of a coagulase-positive Staphylococcus species, which could not be speciated, but showed the same sensitivity as the previous culture...
March 2018: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/29681942/bacterial-profile-antibacterial-resistance-pattern-and-associated-factors-from-women-attending-postnatal-health-service-at-university-of-gondar-teaching-hospital-northwest-ethiopia
#11
Abebaw Bitew Kifilie, Mulat Dagnew, Birhanemeskel Tegenie, Biruk Yeshitela, Rawleigh Howe, Ebba Abate
Introduction: Surgical site infection is a vital cause of maternal mortality and morbidity, especially in resource-limited countries. The rise of antibiotic resistance bacterial infection poses a big threat to this vulnerable population. However, there is lack of studies around the study area. Objective: The purpose of this study was to identify bacterial profile, antibacterial resistance pattern, and associated factors among mothers attending postnatal care health service...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/29675336/bacterial-aggregates-establish-at-the-edges-of-acute-epidermal-wounds
#12
Lene Bay, Kasper N Kragh, Steffen R Eickhardt, Steen S Poulsen, Lise Mette R Gjerdrum, Khaled Ghathian, Henrik Calum, Magnus S Ågren, Thomas Bjarnsholt
Objective: The bacterial composition and distribution were evaluated in acute standardized epidermal wounds and uninjured skin by a molecular in situ technology benchmarked to conventional culturing. This was done to reveal whether bacterial biofilm is present in acute wounds. Approach: On the buttock of 26 healthy volunteers, 28 suction blisters were made and de-roofed. Four wounds were biopsied immediately after wounding, whereas the remaining 24 wounds were treated daily with sterile deionized water and covered with a moisture-retaining dressing...
April 1, 2018: Advances in Wound Care
https://www.readbyqxmd.com/read/29618862/acetic-acid-dressings-finding-the-holy-grail-for-infected-wound-management
#13
Kapil S Agrawal, Anup Vidyadhar Sarda, Raghav Shrotriya, Manoj Bachhav, Vinita Puri, Gita Nataraj
Background: Wounds have since long, contributed majorly to the health-care burden. Infected long-standing non-healing wounds place many demands on the treating surgeon and are devastating for the patients physically, nutritionally, vocationally, financially, psychologically and socially. Acetic acid has long been included among agents used in the treatment of infected wounds. In this study, we have evaluated the use of acetic acid for topical application in the treatment of infected wounds...
September 2017: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
https://www.readbyqxmd.com/read/29615167/pseudomonas-aeruginosa-evaluation-of-pathogen-burden-and-drug-resistance-trends-in-a-tertiary-care-hospital
#14
Muhammad Saeed, Farhan Rasheed, Raja Kamran Afzal, Shahida Hussain, Saba Riaz, Adeel Ahmad
OBJECTIVE: To evaluate the pathogen burden and antibiotic-resistance trends of Pseudomonas aeruginosa among hospitalised patients at a tertiary care hospital. STUDY DESIGN: Retrospective, hospital record-based, cross-sectional study. PLACE AND DURATION OF STUDY: Microbiology Laboratory, Allama Iqbal Medical College/Jinnah Hospital, Lahore, from January 2014 to December 2016. METHODOLOGY: A total of 5,960 samples were collected from clinically suspected cases of bacterial infections, admitted to the hospital...
April 2018: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/29609277/-analysis-of-distribution-and-drug-resistance-of-pathogens-of-burn-patients-during-9-years
#15
Y Dou, Q Zhang
Objective: To investigate distribution and drug resistance of pathogens of burn patients. Methods: A total of 3 357 strains were cultured and isolated from 25 286 specimens of wounds excretion, deep venous catheters, venous blood, stool, mid-stream urine, sputum, puncture fluid, and throat swab of 11 510 burn patients hospitalized in our burn wards from January 2007 to December 2015. After being identified by API bacteria identification panels and automatically bacteria identification equipment, drug-resistances of Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae to 28 common antibiotics were tested by drug sensitivity test with K-B paper disk diffusion method...
March 20, 2018: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/29603518/wound-swab-and-wound-biopsy-yield-similar-culture-results
#16
Marieke Haalboom, Miriam H E Blokhuis-Arkes, Roland J Beuk, Rob Klont, Georg Guebitz, Andrea Heinzle, Job van der Palen
The question remains whether wound swabs yield similar culture results to the traditional gold standard, biopsies. Swabs are not invasive and easy to perform. However, they are believed to capture microorganisms from the surface rather than microorganisms that have invaded tissue. Several studies compared swabs and biopsies using different populations and sampling methods, complicating the ability to draw conclusions for clinical practice. This study aimed to compare swab and biopsy in clinical practice, by including a variety of wounds and using standard sampling and culture procedures...
March 30, 2018: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29541448/multidrug-resistant-gram-negative-bacterial-infections-in-a-teaching-hospital-in-ghana
#17
Nicholas Agyepong, Usha Govinden, Alex Owusu-Ofori, Sabiha Yusuf Essack
Background: Multidrug-resistant Gram-negative bacteria have emerged as major clinical and therapeutic dilemma in hospitals in Ghana.To describe the prevalence and profile of infections attributable to multidrug-resistant Gram-negative bacteria among patients at the Komfo Anokye Teaching Hospital in the Ashanti region of Ghana. Methods: Bacterial cultures were randomly selected from the microbiology laboratory from February to August, 2015. Bacterial identification and minimum inhibitory concentrations were conducted using standard microbiological techniques and the Vitek-2 automated system...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29523908/a-novel-wound-retractor-combining-continuous-irrigation-and-barrier-protection-reduces-incisional-contamination-in-colorectal-surgery
#18
Harry T Papaconstantinou, Rocco Ricciardi, David A Margolin, Roberto Bergamaschi, Robert C Moesinger, Warren E Lichliter, Elisa H Birnbaum
BACKGROUND: Surgical site infection (SSI) remains a persistent and morbid problem in colorectal surgery. Key to its pathogenesis is the degree of intraoperative bacterial contamination at the surgical site. The purpose of this study was to evaluate a novel wound retractor at reducing bacterial contamination. METHODS: A prospective multicenter pilot study utilizing a novel wound retractor combining continuous irrigation and barrier protection was conducted in patients undergoing elective colorectal resections...
March 9, 2018: World Journal of Surgery
https://www.readbyqxmd.com/read/29523721/mrsa-nares-swab-is-a-more-accurate-predictor-of-mrsa-wound-infection-compared-with-clinical-risk-factors-in-emergency-department-patients-with-skin-and-soft-tissue-infections
#19
Nicole M Acquisto, Ryan P Bodkin, Jack E Brown, Paul S Graman, Courtney Marie Cora Jones, Timmy Li, Dwight J Hardy, Elizabeth Dodds Ashley
OBJECTIVES: Skin and soft tissue infections (SSTI) caused by methicillin-resistant Staphylococcus aureus (MRSA) are prevalent in the emergency department (ED). We determined whether MRSA nasal carriage better identifies patients with MRSA wound infection than clinical risk factors or emergency medicine (EM) provider's choice of discharge prescriptions. METHODS: Adult patients presenting to a large academic medical centre ED in the USA with SSTI between May 2010 and November 2011 were screened...
June 2018: Emergency Medicine Journal: EMJ
https://www.readbyqxmd.com/read/29521930/when-and-how-to-perform-cultures-on-chronic-wounds
#20
REVIEW
Yvonne Stallard
PURPOSE: The purpose of this Evidence-Based Report Card was to examine current best evidence related to when and how to perform cultures on chronic wounds to guide clinicians in determining the appropriate treatment. QUESTION: (1) When should cultures be performed on chronic wounds? and (2) What is the best method or technique to perform a culture on a chronic wound? SEARCH STRATEGY: A search of the literature was performed, resulting in 45 publications relevant to the topic...
March 2018: Journal of Wound, Ostomy, and Continence Nursing
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