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Wound swab culture

Natalia Blanco, J Kristie Johnson, John D Sorkin, Alison D Lydecker, Lauren Levy, Lona Mody, Mary-Claire Roghmann
OBJECTIVE: To estimate the risk of transmission of antibiotic-resistant Gram-negative bacteria (RGNB) to gowns and gloves worn by healthcare personnel (HCP) when providing care to residents of community-based nursing facilities to identify the types of care and resident characteristics associated with transmission. DESIGN: Prospective observational study.Settings and participantsResidents and HCP from 13 community-based nursing facilities in Maryland and Michigan...
October 8, 2018: Infection Control and Hospital Epidemiology
M M Rahman, K B Amin, S M M Rahman, A Khair, M Rahman, A Hossain, A K M A Rahman, M S Parvez, N Miura, M M Alam
BACKGROUND: Staphylococcus aureus is responsible for large numbers of hospital-related and community-acquired infections. In this study, we investigated the presence of S. aureus and methicillin-resistant S. aureus (MRSA) in 100 samples from animals (55 cattle, 36 dogs, and 9 cats) and 150 samples from hospitalized human patients. The samples were collected from healthy and diseased animals and from diseased humans and included milk, wound swab, pus, exudates, nasal swab and diabetic ulcer...
October 3, 2018: BMC Veterinary Research
Shankar Srinivasan, Jovita Saldanha, Suhas Abhyankar, Aakansha Patil, Arvind M Vartak
This study aims to assess the change in the antibiotic sensitivity pattern of Pseudomonas species with time. Microbiological data of 2399 patients admitted to the burns unit of the Bai Jerbai Wadia Hospital for Children, Mumbai over a period of 21 years (1994-2014) was reviewed. The age group of patients admitted to our facility ranged from one month to fifteen years. A total of 11,402 burn wound swabs were cultured and 17507 isolates were tested for their antibiotic sensitivity. Pseudomonas was found to be 31...
2018: International Journal of Burns and Trauma
Ian MacNiven, Jason E Hsu, Moni B Neradilek, Frederick A Matsen
Background: Propionibacterium species are commonly cultured from specimens harvested at the time of revision shoulder arthroplasty. These bacteria reside in normal sebaceous glands, out of reach of surgical skin preparation. The arthroplasty incision transects these structures, which allows Propionibacterium to inoculate the wound and to potentially lead to the formation of a biofilm on the inserted implant. To help identify patients who are at increased risk for wound inoculation, we investigated whether preoperative cultures of the specimens from the unprepared skin surface were predictive of the results of intraoperative cultures of dermal wound-edge specimens obtained immediately after incision of the surgically prepared skin...
March 29, 2018: JB & JS open access
Martin Loos, Oliver Strobel, Matthias Legominski, Maximilian Dietrich, Ulf Hinz, Thorsten Brenner, Alexandra Heininger, Markus A Weigand, Markus W Büchler, Thilo Hackert
BACKGROUND: Postoperative pancreatic fistula is a dangerous complication in pancreatic surgery. This study assessed the impact of microbiologic pathogens detected in postoperative pancreatic fistula on clinical outcomes after partial pancreatoduodenectomy and distal pancreatectomy. METHODS: Microorganisms in postoperative pancreatic fistula were identified by microbiologic analyses from abdominal drains or intraoperative swabs during relaparotomy. Demographic, operative, and microbiologic data, as well as postoperative outcomes were examined...
September 11, 2018: Surgery
Rosemary Hill, Monique Y Rennie, Joshua Douglas
The urgent need to eliminate unnecessary use of antibiotics in wound patients has been hampered by diagnostic uncertainty and the time required to obtain culture results. The authors evaluated bedside use of a handheld bacterial fluorescence imaging device for real-time visualization of bacteria within and around wounds, used in addition to monitoring of clinical signs and symptoms of infection, in a series of 7 patients (5 women, 2 men; age range 57-93 years) with varying comorbidities who were referred to the wound ostomy continence clinician for wound assessment...
August 2018: Ostomy/wound Management
Eguono Erhinyaye Omoyibo, Ayodeji Olarewaju Oladele, Muhammad Habib Ibrahim, Oluwakayode Temitope Adekunle
Background: Wounds are commonly encountered in the clinical practice. Microbacterial colonization and infection negatively affect wound outcomes. With increasing emergence of antibiotic-resistant strains, it is essential to determine local patterns of wound microbiological profile and antibiotic susceptibility to guide rational empirical antibiotic use. Materials and Methods: Consecutive patients who presented to the plastic surgery unit were recruited to the study over a 6-month period...
July 2018: Annals of African Medicine
M Haalboom, M H E Blokhuis-Arkes, R J Beuk, R Meerwaldt, R Klont, M J Schijffelen, P B Bowler, M Burnet, E Sigl, J A M van der Palen
OBJECTIVES: The aim of this study was to determine whether assessment of wound infection differs when culture results from wound biopsy versus wound swab are available in clinical practice. METHODS: For 180 eligible patients, a swab and biopsy were taken from one wound during a regular appointment at a wound care facility in eastern Netherlands. Culture results from both methods were supplemented with clinical information and provided to a panel of six experts who independently assessed each wound as infect or not, separately for swab and biopsy...
August 24, 2018: Clinical Microbiology and Infection
Vincent Prinz, Simon Bayerl, Nora Renz, Andrej Trampuz, Peter Vajkoczy, Tobias Finger
BACKGROUND: Antimicrobial treatment of ventriculoperitoneal (VP) shunt infections is challenging when the causative pathogen is unknown. OBJECTIVE: To evaluate the value of sonication of explanted shunt-devices to improve the microbiological detection rate. METHODS: All consecutive patients undergoing revision surgery due to suspected VP-shunt infection from January 2015 to February 2017 were evaluated. Intraoperative tissue samples, wound swabs, and cerebrospinal fluid (CSF) were collected for microbiological examination...
August 17, 2018: Neurosurgery
Priscila R Oliveira, Marcos C Leonhardt, Vladimir C Carvalho, Kodi E Kojima, Jorge S Silva, Flavia Rossi, Ana Lucia L Lima
BACKGROUND: Diaphyseal fractures of femur and tibia are prominent due to its high incidence and high economic and social impact. Intramedullary nailing (IN) is the surgical procedure of choice. Surgical site infection (SSI) related to this procedure is considered a difficult to treat complication. AIMS: Determine the incidence of SSI after IN of femoral and tibial diaphyseal fractures and evaluate possible risk factors. METHODS: Prospective observational cohort study...
October 2018: Injury
Reza Ghotaslou, Mohammad Yousef Memar, Naser Alizadeh
Diabetic foot ulcers (DFUs) are a common complication of type-1 and type-2 diabetes. About 10-15% of patients with diabetes develop foot ulcers. A validated foot ulcer classification system that will support the development of treatment strategis is necessary for clinicians managing DFUs. More than 10 classification systems have been described by researchers. Another important aspect of the management of DFUs is the proper identification of causative pathogens that trigger infections. While conventional diagnostic methods, such as swabs, cultures and biopsies are more widely used, novel molecular techniques have been exploring bacterial identification and quantification...
July 2, 2018: Journal of Wound Care
Theckla Kazimoto, Salim Abdulla, Leah Bategereza, Omar Juma, Francis Mhimbira, Maja Weisser, Jürg Utzinger, Lutz von Müller, Sören L Becker
Few epidemiological studies have been carried out to assess the aetiology and antimicrobial susceptibility patterns of pathogens giving rise to skin and soft tissue infections (SSTIs) in sub-Saharan Africa. In the present study from six healthcare facilities in Bagamoyo, Tanzania, wound swabs from outpatients with SSTIs were analysed by a suite of methods, including microbiological culture techniques, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and resistance testing. Among 185 patients with SSTIs, 179 (96...
October 2018: Acta Tropica
Shaal Ramdial, Anil Madaree
Dehiscence or palatal fistula formation following palatoplasty is a complication that has grave consequences for the patient that include tissue loss, emotional distress to the parents and patient, and further medical costs. Palatal dehiscence or fistula formation is multifactorial following surgery-tension of wound closure, poor patient adherence to postoperative orders and wound infection are the most common causes for this. Oral colonization with pathogenic organisms could play a role in wound healing complications...
June 27, 2018: MicrobiologyOpen
Toshitaka Yoshii, Takashi Hirai, Tsuyoshi Yamada, Kenichiro Sakai, Shuta Ushio, Satoru Egawa, Masato Yuasa, Tsuyoshi Kato, Hiroyuki Inose, Shigenori Kawabata, Atsushi Okawa
STUDY DESIGN: This is a prospective comparative study. OBJECTIVE: We evaluated the efficacy of 2 standard antiseptic solutions, chlorhexidine-gluconate (CHG) and povidone-iodine (PD-I), in eliminating bacterial pathogens from surgical sites in posterior spine surgeries. SUMMARY OF BACKGROUND DATA: Previous studies have shown that CHG is more effective for skin antisepsis than PD-I in joint surgeries. However, few studies have investigated the preoperative use of antiseptic solutions in spine surgery...
August 2018: Clinical Spine Surgery
Sileshi Tadesse, Haile Alemayehu, Admasu Tenna, Getachew Tadesse, Tefaye Sisay Tessema, Workineh Shibeshi, Tadesse Eguale
BACKGROUND: Staphylococcus aureus is one of the major pathogens of public health importance responsible for various forms of infection. Development of resistance to commonly used antimicrobials limited treatment options against infections due to this pathogen. Antimicrobial resistance profile of Staphylococcus aureus isolated from patients with surgical site infection and ear infection and corresponding nasal swab was investigated in Tikur Anbessa Specialized Hospital (TASH), Addis Ababa, Ethiopia...
May 21, 2018: BMC Pharmacology & Toxicology
Bahattin Kemah, Gökçer Uzer, Yalçın Turhan, Burak Özturan, Bülent Kılıç, Bilge Sümbül Gültepe, Ayşe Bahar Ceyran, Selim Ertürk, Burak Aksoylu, Özlem Şenaydın, Korhan Özkan
Purpose : Bone and soft tissue infections are among the least desired complications after orthopaedic surgery. This study analysed the in vivo effects of the local application of nano-silver particles (AgNPs) [1nm = 1 billionth of a meter] in soft tissue infections. Materials-Method : An experimental osteomyelitis model was formed by inoculating both tibias of 24 rats with methicillin-resistant Staphylococcus aureus . The rats were followed without treatment for 21 days. Blood samples and tibial x-rays at day 21 confirmed the development of infection...
2018: Journal of Bone and Joint Infection
Michel Roethlisberger, Giusi Moffa, Urs Fisch, Benedikt Wiggli, Stephan Schoen, Christopher Kelly, Severina Leu, Davide Croci, Daniel W Zumofen, Nadine Cueni, Danica Nogarth, Marianne Schulz, Heiner C Bucher, Maja Weisser-Rohacek, Morten-Goetz Wasner, Andreas F Widmer, Luigi Mariani
Background: Observational studies have shown that dressings containing chlorhexidine gluconate (CHX) lower the incidence external ventricular drain (EVD) associated infections (EVDAI). This prospective, randomized controlled trial (RCT) studies the efficacy of CHX-containing dressings in reducing bacterial colonization, which represents an important risk factor for infectious complications. Methods: In this single-site, prospective, double-blinded RCT, patients aged ≥18 undergoing emergency EVD placement were randomly given either a CHX- or an otherwise identical control dressing at the skin exit wound of the EVD...
May 4, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Selina Winter, Mirja Christine Nolff, Sven Reese, Andrea Meyer-Lindenberg
OBJECTIVE: To evaluate the bacterial contamination rate and to compare the efficacy of polyhexanide, cold argon plasma and saline at reducing bacterial bio-burden in dog bite wounds. MATERIAL AND METHODS: Dogs with bite-wound injuries were included when surgical debridement was pursued with subsequent treatment using either polyhexanide-biguanide lavage (A), cold argon plasma treatment (B) or saline lavage (C). Culture swabs were taken after debridement as well as after lavageor argon treatment...
April 2018: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
Anthony A Pilny
A 23-year-old, 425-g male African grey parrot ( Psittacus erithacus) was evaluated for chronic ulcerative dermatitis of the axillary regions under both wings. Initial swab cultures of the sites had revealed a coagulase-positive methicillin-resistant Staphylococcus aureas (MRSA) with marked antibiotic resistance. A second swab culture obtained 8 weeks after the initial culture showed heavy growth of a coagulase-positive Staphylococcus species, which could not be speciated, but showed the same sensitivity as the previous culture...
March 2018: Journal of Avian Medicine and Surgery
Abebaw Bitew Kifilie, Mulat Dagnew, Birhanemeskel Tegenie, Biruk Yeshitela, Rawleigh Howe, Ebba Abate
Introduction: Surgical site infection is a vital cause of maternal mortality and morbidity, especially in resource-limited countries. The rise of antibiotic resistance bacterial infection poses a big threat to this vulnerable population. However, there is lack of studies around the study area. Objective: The purpose of this study was to identify bacterial profile, antibacterial resistance pattern, and associated factors among mothers attending postnatal care health service...
2018: International Journal of Microbiology
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