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Swab culture

Le Thi Quynh Nhi, Ha Thanh Tuyen, Pham Duc Trung, Tran Do Hoang Nhu, Pham Thanh Duy, Chung The Hao, Nguyen Thi Thanh Nhan, Lu Lan Vi, Hoang Thi Diem Tuyet, Tran Thi Thuy Tien, Nguyen Van Vinh Chau, Phung Khanh Lam, Guy Thwaites, Stephen Baker
PURPOSE: Antimicrobial-resistant bacterial infections in low- and middle-income countries (LMICs) are a well-established global health issue. We aimed to assess the prevalence of and epidemiological factors associated with the carriage of ciprofloxacin- and ceftriaxone-resistant Escherichia coli and associated resistance genes in a cohort of 498 healthy children residing in urban Vietnam. METHODOLOGY: We cultured rectal swabs onto MacConkey agar supplemented with resistant concentrations of ciprofloxacin and ceftriaxone...
August 16, 2018: Journal of Medical Microbiology
Minyi Zhu, Chao Cheng, Haisu Yi, Liping Lin, Kaili Wu
Bacterial infection or Demodex infestation has been reported to contribute to chronic blepharitis. The association between Demodex mites and bacterial flora in this disease remains to be elucidated. Fifty-six consecutive patients diagnosed with chronic blepharitis and 46 healthy volunteers were recruited for this study. Using specimens of three epilated eyelashes and lid margin swabs, Demodex were identified microscopically and bacteria were determined by cultures, followed by colony counting and mass spectrometry...
2018: Frontiers in Microbiology
Minal M Amin, Jennifer D Stowell, William Hendley, Philip Garcia, D Scott Schmid, Michael J Cannon, Sheila C Dollard
BACKGROUND: Caring for young children is a known risk factor for cytomegalovirus (CMV) infection mainly through exposure to their saliva and urine. In a previous study, 36 CMV-seropositive children 2 mo. to 4 years old were categorized as CMV shedders (n = 23) or non-shedders (n = 13) based on detection of CMV DNA in their saliva and urine. The current study evaluated the presence of CMV on surfaces in homes of the children. METHODS: Study staff made 4 visits to homes of the 36 enrolled children over 100 days...
August 13, 2018: BMC Infectious Diseases
Yuanting Tang, Fan Yu, Lairong Huang, Zhengqiang Hu
OBJECTIVE: This study was to investigate the changes of antifungal susceptibilities caused by the phenotypic switching in patients with vulvovaginal candidiasis (VVC). METHODS: 229 women were enrolled in this study. The vaginal smears of these patients were collected and gram stained for fungal microscopic observation. The vaginal discharge of them in cotton swabs was cultured in sabouraud's agar with chloramphenicol medium. After fungal culture, fungal identification was analyzed using CHROM agar Candida chromogenic and identification medium...
August 12, 2018: Journal of Clinical Laboratory Analysis
Yasser S Mahmmod, Bettina Nonnemann, Line Svennesen, Karl Pedersen, Ilka Christine Klaas
Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF), a culture-dependent assay, has recently been implemented for routine identification of non-aureus staphylococci (NAS) species from milk, but the assay has never been investigated for NAS from nonmilk or environmental samples. The objective of this study was to evaluate the typeability of the MALDI-TOF assay for the identification and differentiation of bovine-associated NAS species on aseptically collected quarter milk and teat skin samples in dairy herds...
August 9, 2018: Journal of Dairy Science
Jessica Nina Büttner, Marianne Schneider, Jaqueline Csokai, Elisabeth Müller, Johanna Corinna Eule
OBJECTIVES: To describe the bacterial and fungal microbiota of the conjunctiva and factors influencing these microbiota of healthy cats. To evaluate the antimicrobial resistance profile and discuss the use of appropriate antimicrobial drugs. ANIMALS STUDIED: One hundred and twenty healthy cats PROCEDURES: Conjunctival samples with dry or premoistened swabs were obtained from both eyes and cultured for aerobic and anaerobic bacteria and fungi. In vitro bacterial, susceptibility testing was performed...
August 10, 2018: Veterinary Ophthalmology
Dale N Gerding, Susan P Sambol, Stuart Johnson
The beneficial effect of colonization of the gastrointestinal tract by non-toxigenic Clostridioides difficile (NTCD) strains as a preventive of toxigenic C. difficile infection (CDI) has been known since the early 1980s. Investigators in both the USA and United Kingdom demonstrated that prior colonization by randomly selected NTCD strains provided prevention against infection by toxigenic C. difficile in hamsters, albeit with limited durability. In the 1980s two patients with multiply recurrent CDI in the UK were treated with vancomycin followed by NTCD to prevent further recurrences, with one success and one failure...
2018: Frontiers in Microbiology
Juraj Korbelik, Ameet Singh, Joyce Rousseau, J Scott Weese
BACKGROUND: Otitis externa is a common multifactorial disease with a prevalence in dogs as high as 10-20%. In humans, the diversity of the cutaneous mycobiota appears to increase in diseased states, whereas one canine study identified a decrease in diversity of the cutaneous mycobiota in atopic dogs compared to healthy individuals. HYPOTHESIS/OBJECTIVES: To describe the otic mycobiota in dogs with otitis externa compared to healthy dogs. ANIMALS: Samples were collected from six dogs with clinical and cytological evidence of otitis externa and five clinically normal dogs...
August 8, 2018: Veterinary Dermatology
Maryam Niyyati, Alireza Naghahi, Hamed Behniafar, Zohreh Lasjerdi
Background: The presence of potentially pathogenic Free Living Amoebae (FLA) in hospital environment could be a health hazard for high-risk patients such as immunosuppressed patients. This study was carried out to investigate the presence of potentially pathogenic FLAs in the environment and medical instruments of different hospital wards, and nasal swabs of immunosuppressed patients of a hospital in Tehran, Iran. Methods: In this cross-sectional study, 60 environmental (26 samples) and nasal swab (34 samples) samples were collected between Dec 2015 and Feb 2016...
June 2018: Iranian Journal of Public Health
Priscila R Oliveira, Marcos C Leonhardt, Vladimir C Carvalho, Kodi E Kojima, Jorge S Silva, Flavia Rossi, Ana Lucia L Lima
BACKGROUND: Diaphyseal fractures of femur and tibia are prominent due to its high incidence and high economic and social impact. Intramedullary nailing (IN) is the surgical procedure of choice. Surgical site infection (SSI) related to this procedure is considered a difficult to treat complication. AIMS: Determine the incidence of SSI after IN of femoral and tibial diaphyseal fractures and evaluate possible risk factors. METHODS: Prospective observational cohort study...
July 27, 2018: Injury
Adam P Raya, David A Jaffe, Bruno B Chomel, Marissa S Ota, Peter M Tsou, Aaron Z Davis, José Ignacio Olave-Leyva, Guillermo Galvez-Romero, Matthew J Stuckey, Rickie W Kasten, Cirani Obregón-Morales, Nidia Aréchiga-Ceballos, Flor Martinez-Martinez, Alvaro Aguilar-Setién
Bartonella spp. have been identified in many bat species worldwide, including the zoonotic species, Candidatus Bartonella mayotimonensis. The common vampire bat (Desmodus rotundus) preys preferentially on livestock in Latin America and is frequently infected with Bartonella spp. To determine the potential role of D. rotundus in transmitting Bartonella to livestock, common vampire bats and bat-bitten domestic ruminants from Mexico were tested for Bartonella infection by blood culture or conventional PCR. Furthermore, to explore the possibility of bite transmission during blood feeding, saliva swabs from 35 D...
August 2018: Veterinary Microbiology
U U Aniebue, T O Nwankwo, M I Nwafor
Background: Clinical diagnosis of acute vulvovaginal candidiasis (VVC) depends on evidence of clinical symptoms, but symptomatic treatment widely practiced in low-resource area may lead to overdiagnosis and treatment. Objective: The objective of the study is to determine the prevalence of VVC among women attending gynecological clinic in University of Nigeria Teaching Hospital (UNTH) Enugu and the accuracy of clinical-based diagnosis versus laboratory test supported diagnosis and patients' characteristics that affect accuracy...
August 2018: Nigerian Journal of Clinical Practice
Sara C Bell, Stephen Garland, Ross A Alford
Symbiotic bacterial communities resident on amphibian skin can benefit their hosts. For example, antibiotic production by community members can control the pathogen Batrachochytrium dendrobatidis ( Bd ) and it is possible for these community members to be used as probiotics to reduce infection levels. In the early 1990s, the emergence of Bd caused declines and disappearances of frogs in the Australian Wet Tropics; the severity of its effects varied among species and sites. Some species have since recolonized despite enzootic Bd within their populations...
2018: Frontiers in Microbiology
Alexandra E Livanos, Erik J Snider, Susan Whittier, David H Chong, Timothy C Wang, Julian A Abrams, Daniel E Freedberg
Commensal gastrointestinal bacteria resist the expansion of pathogens and are lost during critical illness, facilitating pathogen colonization and infection. We performed a prospective, ICU-based study to determine risk factors for loss of gut colonization resistance during the initial period of critical illness. Rectal swabs were taken from adult ICU patients within 4 hours of admission and 72 hours later, and analyzed using 16S rRNA gene sequencing and selective culture for vancomycin-resistant Enterococcus (VRE)...
2018: PloS One
Mark R Hobbs, Cameron C Grant, Mark G Thomas, Sarah Berry, Susan M B Morton, Emma Marks, Stephen R Ritchie
New Zealand children suffer from high rates of skin and soft tissue infection (SSTI). Staphylococcus aureus colonisation is known to increase the risk of nosocomial infection. We aimed to determine whether S. aureus colonisation also increased the risk of community-onset SSTI. This study, performed within the Growing Up in New Zealand cohort, used interview and administrative data, and bacterial culture results from the nose, throat, and skin swabs collected at 4½ years of age. Multivariable log-binomial regression was used to derive adjusted risk ratios...
July 31, 2018: European Journal of Clinical Microbiology & Infectious Diseases
H Chen, K M Au, K E Hsu, C K Lai, J Myint, Y F Mak, S Y Lee, T Y Wong, N C Tsang
INTRODUCTION: A point prevalence survey was conducted to study the epidemiology of and risk factors associated with multidrug-resistant organism carriage among residents in residential care homes for the elderly (RCHEs). METHODS: A total of 20 RCHEs in Hong Kong were selected by stratified single-stage cluster sampling. All consenting residents aged ≥65 years from the selected RCHEs were surveyed by collection of nasal swab, axillary swab, rectal swab or stool on one single day for each home...
July 30, 2018: Hong Kong Medical Journal, Xianggang Yi Xue za Zhi
Steve Horvath, Junko Oshima, George M Martin, Ake T Lu, Austin Quach, Howard Cohen, Sarah Felton, Mieko Matsuyama, Donna Lowe, Sylwia Kabacik, James G Wilson, Alex P Reiner, Anna Maierhofer, Julia Flunkert, Abraham Aviv, Lifang Hou, Andrea A Baccarelli, Yun Li, James D Stewart, Eric A Whitsel, Luigi Ferrucci, Shigemi Matsuyama, Kenneth Raj
DNA methylation (DNAm)-based biomarkers of aging have been developed for many tissues and organs. However, these biomarkers have sub-optimal accuracy in fibroblasts and other cell types used in ex vivo studies. To address this challenge, we developed a novel and highly robust DNAm age estimator (based on 391 CpGs) for human fibroblasts, keratinocytes, buccal cells, endothelial cells, lymphoblastoid cells, skin, blood, and saliva samples. High age correlations can also be observed in sorted neurons, glia, brain, liver, and even bone samples...
July 26, 2018: Aging
R Selvaganapathi, G Jeyasekaran, R Jeya Shakila, D Sukumar, M Palani Kumar, B Sivaraman
Listeria monocytogenes was screened from different seafood contact surfaces in five sampling sites of fishing harbour, fish landing centers, seafood processing plants, fish market, and fish curing yards of Tuticorin Coast of India. 115 swab samples were collected and tested for the occurrence of L. monocytogenes by conventional and molecular methods. Overall, 5.22% of swab samples collected were positive for L. monocytogenes . The fishing harbour had high incidence (10.3%) of L. monocytogenes followed by fish landing centers (5...
July 2018: Journal of Food Science and Technology
Eric Leis, Rebekah McCann, Isaac Standish, Anna Bestul, Torri Odom, Casey Finnerty, Barb Bennie
Traditional methodologies to identify fish pathogens require euthanasia before the collection of tissue samples. While these methods are standardized and proven, there are instances where nonlethal alternatives would be preferred. Despite the need to develop nonlethal sampling techniques, few publications have focused on them and even fewer have used these approaches to identify viruses from infections occurring in wild fish populations. In this study, we compared the ability of nonlethal sampling techniques with traditional methods for the detection of Largemouth Bass virus (LMBV) from a wild population of Largemouth Bass Micropterus salmoides from the upper Mississippi River...
July 24, 2018: Journal of Aquatic Animal Health
Susan E Kline, James D Neaton, Ruth Lynfield, Patricia Ferrieri, Shalini Kulasingam, Kayleigh Dittes, Anita Glennen, Selina Jawahir, Alexander Kaizer, Jeremiah Menk, James R Johnson
OBJECTIVE: To determine the efficacy in eradicating Staphylococcus aureus (SA) carriage of a 5-day preoperative decolonization bundle compared to 2 disinfectant soap showers, with both regimens self-administered at home. DESIGN: Open label, single-center, randomized clinical trial. SETTING: Ambulatory orthopedic, urologic, neurologic, colorectal, cardiovascular, and general surgery clinics at a tertiary-care referral center in the United States...
July 24, 2018: Infection Control and Hospital Epidemiology
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