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https://www.readbyqxmd.com/read/30333815/a-comparison-of-techniques-for-collecting-skin-microbiome-samples-swabbing-versus-tape-stripping
#1
Kazuhiro Ogai, Satoshi Nagase, Kanae Mukai, Terumi Iuchi, Yumiko Mori, Miki Matsue, Kayo Sugitani, Junko Sugama, Shigefumi Okamoto
The swabbing and tape-stripping methods have traditionally been used for collecting skin microbiome samples for skin bacterial analysis, although no reports have compared the outcome of these methods for collecting skin bacteria. Our purpose was to show the differences in microbial composition between samples collected using the swabbing and tape-stripping methods, by both the next generation sequencing and culture studies. The skin microbiome was collected by both methods, and the samples were processed for a sequence-based microbiome analysis and culture study...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30327672/risk-factors-for-vaginal-colonization-and-relationship-between-bacterial-vaginal-colonization-and-in-hospital-outcomes-in-women-with-obstructed-labor-in-a-ugandan-regional-referral-hospital
#2
Joseph Ngonzi, Lisa M Bebell, Joel Bazira, Yarine Fajardo, Dan Nyehangane, Yap Boum, Deborah Nanjebe, Adeline Boatin, Jerome Kabakyenga, Yves Jacquemyn, Jean-Pierre Van Geertruyden, Laura E Riley
Introduction: The proportion of women with severe maternal morbidity from obstructed labor is between 2 and 12% in resource-limited settings. Maternal vaginal colonization with group B streptococcus (GBS), Escherichia coli , and Enterococcus spp. is associated with maternal and neonatal morbidity. It is unknown if vaginal colonization with these organisms in obstructed labor women is associated with poor outcomes. Objectives: To determine whether vaginal colonization with GBS, E...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/30321312/the-skin-microbiota-in-patients-hospitalized-for-cellulitis-and-association-with-outcome
#3
Duncan R Cranendonk, Floor Hugenholtz, Jan M Prins, Paul H M Savelkoul, Andries E Budding, W Joost Wiersinga
Background: The skin microbiota plays a key role in the pathogenesis of several skin diseases, but its role in cellulitis remains unknown. We investigated the skin microbiota in patients with cellulitis, studied whether its analysis could help determine the causative pathogen, and explored whether skin microbiota composition was associated with clinical outcomes. Methods: We prospectively included 58 patients hospitalized for cellulitis. Skin swabs obtained from the lesion sites were compared with swabs from identical sites on the contralateral unaffected limbs and with swabs obtained from 19 age- and sex-matched control subjects without cellulitis...
October 13, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/30315956/evaluation-of-rectal-swab-use-for-the-determination-of-enteric-pathogens-a-prospective-study-of-community-acquired-diarrhoea-in-adults
#4
Kotar Tadeja, Pirš Mateja, Steyer Andrej, Cerar Tjaša, Šoba Barbara, Skvarc Miha, Poljšak Prijatelj Mateja, Lejko Zupanc Tatjana
OBJECTIVES: A stool sample is a sample of choice for microbiological testing of enteric pathogens causing diarrhoea, but a rectal swab can be a more practical alternative. A prospective observational study was performed to evaluate the diagnostic performance of flocked rectal swab specimens using the syndromic molecular approach to determine the aetiology of diarrhoea in adults. METHODS: We compared the performance of rectal swabs to stool samples as the gold standard in determining viral, bacterial and protozoal pathogens using real-time multiplex PCR as well as standard stool culture...
October 10, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/30315702/isolation-of-chlamydia-spp-from-ewes-and-does-in-iran
#5
H Esmaeili, M Hamedi, S A Madani
Enzootic ovine abortion is caused by Chlamydia abortus and may result in abortion among small ruminants during the last 2-3 weeks of pregnancy. Enzootic abortion is diagnosed by isolation of the agent or detection of its nucleic acid in the products of abortion or vaginal excretions of freshly aborted females. Isolation of chlamydial agents in cell culture is the gold standard, so in the present study this method was employed. Twenty-eight vaginal and conjunctival swab samples were selected from ewes and does that had recently aborted...
December 2017: Archives of Razi Institute
https://www.readbyqxmd.com/read/30315701/detection-and-isolation-of-mycoplasma-capricolum-subspecies-capricolum-from-east-azerbaijan-sheep-flocks
#6
A Jafarizadeh, S A Pourbakhsh, K Tadayon, M Jamshidian
Mycoplasma capricolum subspecies capricolum (Mcc) is one of the causative agents of contagious agalactia (CA), which is an important disease in sheep and goats in the Mediterranean and Middle East countries. Mycoplasma agalactiae is the classic agent of CA in sheep and goats. Mycoplasma mycoides subspecies Capri (Mmc), Mycoplasma capricolum subspecies capricolum (Mcc), and Mycoplasma putrefaciens (Mp) produce a clinically similar disease, more often in goats. The aim of the present study was to detect Mcc in sheep flocks in East Azerbaijan Province of Iran...
December 2017: Archives of Razi Institute
https://www.readbyqxmd.com/read/30315697/isolation-and-identification-of-mycoplasma-agalactiae-by-culture-and-polymerase-chain-reaction-methods-in-the-sheep-herds-in-guilan-province-iran
#7
E Rahimabadi, Y Asadpour, S A Pourbakhsh, P Sayehban
Contagious agalactia is an infectious syndrome of sheep that is characterized by mastitis with reduction of milk production, arthritis, abortion, and keratoconjunctivitis. The disease is rapidly spread by the contact of the infected animals with the healthy ones. Domestic sheep and goats of both sexes can be infected at an equivalent frequency. Most of the researchers use culture and molecular methods for the isolation and identification of Mycoplasma. Mycoplasma agalactiae is the main cause of the disease in sheep...
December 2017: Archives of Razi Institute
https://www.readbyqxmd.com/read/30315328/uselessness-of-microbiological-samples-in-acute-appendicitis-with-frank-pus-to-collect-or-not-to-collect
#8
Mauro Montuori, Letizia Santurro, Luca Gianotti, Luca Fattori
INTRODUCTION: Current use of antimicrobial therapy is prophylactic, empirical and broad spectrum. But, the age-old practice of obtaining cultures still remain. The aim of this study was to evaluate bacterial etiology and adequacy of antibiotic prophylaxis in patients diagnosed with acute appendicitis to help determine the utility of intraoperative cultures in guiding clinical decision-making. MATERIALS AND METHODS: A retrospective analysis of a prospectively constructed database of all patients who underwent appendectomy from September 2013 to November 2016 was performed...
October 12, 2018: European Journal of Trauma and Emergency Surgery: Official Publication of the European Trauma Society
https://www.readbyqxmd.com/read/30313125/factors-influencing-the-contamination-rates-of-the-conjunctival-swabs-and-organ-culture-media-of-human-donor-eyes-erratum
#9
(no author information available yet)
No abstract text is available yet for this article.
October 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/30311533/effects-of-topical-ophthalmic-application-of-0-5-proparacaine-hydrochloride-on-aerobic-bacterial-culture-results-for-naturally-occurring-infected-corneal-ulcers-in-dogs
#10
Katelyn E Fentiman, Amy J Rankin, Jessica M Meekins, James K Roush
OBJECTIVE To evaluate the effects of topical ophthalmic application of 0.5% proparacaine hydrochloride solution (PHCL; containing 0.01% benzalkonium chloride as preservative) on aerobic bacterial culture results for naturally occurring infected corneal ulcers in dogs. DESIGN Clinical trial. ANIMALS 25 client-owned dogs with infected corneal ulcers (24 unilaterally affected and 1 bilaterally affected; only 1 eye included/dog) examined between June 2008 and May 2011. PROCEDURES Swab samples for aerobic bacterial culture were collected from the periphery of each corneal ulcer before and approximately 1 minute after topical ophthalmic application of 1 drop of PHCL...
November 1, 2018: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/30307844/are-bacterial-culture-quantifications-reliable-comparative-performance-of-the-wasp-automated-inoculation-instrument-in-the-era-of-iso-15189-accreditation
#11
V Fihman, S C Bleunven, J M Le Glaunec, F Maillebuau, I De Rochebouet, B Nebbad-Lechani, M Desroches, J W Decousser
PURPOSE: Isolating colonies and obtaining accurate colony counts from bacterial cultures are critical steps for the optimal management of infected patients. The uncertainties in the colony count results from the bacterial cultures were evaluated by verifying the performance of the WASP inoculation system according to the International Organization for Standardization (ISO) 15189 standard. METHODOLOGY: We first (i) evaluated the cross-contamination and precision of the WASP instrument (Copan Diagnostics, Italy) and (ii) established enumeration reading grids for urine, swab, bronchopulmonary specimens (BPSs) and catheter tip cultures...
October 11, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30307573/no-association-of-polymorphisms-in-nav1-7-or-nerve-growth-factor-receptor-genes-with-trigeminal-neuralgia
#12
Grazielle Mara Ferreira Costa, Luiz Paulo C Rocha, Silvia Regina Dowgan Tesseroli de Siqueira, Paula Rocha Moreira, Camila Megale Almeida-Leite
Objective: Trigeminal neuralgia is defined as a sudden severe shock-like pain within the distribution of the trigeminal nerve. Pain is a subjective experience that is influenced by gender, culture, environment, psychological traits, and genes. Sodium channels and nerve growth factor play important roles in the transmission of nociceptive signals and pain. The aim of this study was to investigate the occurrence of Nav1.7 sodium channel and nerve growth factor receptor TrkA gene polymorphisms (SCN9A/rs6746030 and NTRK1/rs633, respectively) in trigeminal neuralgia patients...
October 10, 2018: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/30305206/validation-of-the-listeria-right-now-tm-test-for-detection-of-listeria-spp-from-selected-environmental-surfaces-without-enrichment
#13
Quynh-Nhi Le, Susan Alles, Brooke Roman, Eric Tovar, Edan Hosking, Lei Zhang, Preetha Biswas, Benjamin Bastin, Patrick M Bird, Mark Mozola, Robert Donofrio
Background: Listeria Right NowTM is a novel, enrichment-free molecular method for detection of Listeria spp. in swab samples from environmental surfaces. The test provides results in real time, indicating the current or recent presence of Listeria spp. in the environment. After sampling, the entire contents of the swab are subject to sample processing, releasing large quantities of target ribosomal RNA molecules into the lysate. A portion of the lysate is then tested using the ANSR® for Listeria isothermal nucleic acid amplification assay...
October 10, 2018: Journal of AOAC International
https://www.readbyqxmd.com/read/30304619/association-of-infections-with-the-use-of-a-temporary-double-lumen-catheter-for-hemodialysis
#14
Viviane Ferreira, Miguel Moysés Neto, Jose Abrão Cardeal da Costa
The goal of this study was to identify the occurrence of secondary infection due to the use of temporary double-lumen catheters for hemodialysis in 96 patients with acute kidney injury or acute chronic kidney disease. For each patient, we collected a swab from the skin localized in the insertion of catheter before antisepsis; blood cultures from the catheter lumen, peripheral vein, and central venous access in withdrawing catheter; and cultures of catheter tips. Catheters were implanted into a femoral vein in 56...
May 2018: Nephrology Nursing Journal: Journal of the American Nephrology Nurses' Association
https://www.readbyqxmd.com/read/30298131/a-fatal-bacteremia-caused-by-hypermucousviscous-kpc-2-producing-extensively-drug-resistant-k64-st11-klebsiella-pneumoniae-in-brazil
#15
Tatiana Amabile de Campos, Laura Fernandes Gonçalves, Kelly Grace Magalhães, Vicente de Paulo Martins, Georgios Joannis Pappas Júnior, Gisele Peirano, Johann D D Pitout, Guilherme Bartolomeu Gonçalves, João Pedro Rueda Furlan, Eliana Guedes Stehling, André Pitondo-Silva
We report a fatal bacteremia caused by Klebsiella pneumoniae in a 60-70-year-old patient from Brazil. The genomic analysis of three isolates (from blood culture, nasal and anal swabs) showed that the bacteremia was caused by a KPC-2 producing extensively drug-resistant K64-ST11 hypermucousviscous K. pneumoniae (hmKP) harboring several virulence and antimicrobial resistance genes. Although the isolates did not present virulence markers associated with hypervirulent K. pneumoniae (hvKP), they showed invasion and toxicity to epithelial Hep-2 cells; resistance to cell microbicidal mechanisms; and blood and human serum survival, evidencing their pathogenic potential...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/30294868/benzoyl-peroxide-chlorhexidine-versus-chlorhexidine-alone-skin-preparation-to-reduce-propionibacterium-acnes-a-randomized-controlled-trial
#16
Douglas S Hancock, Shavantha L Rupasinghe, Ilia Elkinson, Max G Bloomfield, Peter D Larsen
BACKGROUND: Traditional skin preparation for shoulder surgery is not specific for Propionibacterium acnes. Topical benzoyl peroxide for 48 h preoperatively has been shown to reduce the bacterial load of P. acnes on the skin. Our aim was to investigate whether skin preparation with a single application of benzoyl peroxide combined with 2% chlorhexidine/alcohol immediately prior to surgery was superior to 2% chlorhexidine/alcohol alone at inhibiting P. acnes. METHODS: We conducted a single-blinded interventional study...
October 8, 2018: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/30293533/transmission-of-resistant-gram-negative-bacteria-to-healthcare-personnel-gowns-and-gloves-during-care-of-residents-in-community-based-nursing-facilities
#17
Natalia Blanco, J Kristie Johnson, John D Sorkin, Alison D Lydecker, Lauren Levy, Lona Mody, Mary-Claire Roghmann
OBJECTIVE: To estimate the risk of transmission of antibiotic-resistant Gram-negative bacteria (RGNB) to gowns and gloves worn by healthcare personnel (HCP) when providing care to residents of community-based nursing facilities to identify the types of care and resident characteristics associated with transmission. DESIGN: Prospective observational study.Settings and participantsResidents and HCP from 13 community-based nursing facilities in Maryland and Michigan...
October 8, 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/30286741/drug-resistant-enterobacteriaceae-colonization-is-associated-with-healthcare-utilization-and-antimicrobial-use-among-inpatients-in-pune-india
#18
Renu Bharadwaj, Matthew L Robinson, Usha Balasubramanian, Vandana Kulkarni, Anju Kagal, Priyanka Raichur, Sandhya Khadse, Dileep Kadam, Chhaya Valvi, Aarti Kinikar, Savita Kanade, Nishi Suryavanshi, Ivan Marbaniang, George Nelson, Julia Johnson, Jonathan Zenilman, Jonathan Sachs, Amita Gupta, Vidya Mave
BACKGROUND: Healthcare exposure may increase drug-resistant Enterobacteriaceae colonization risk. Nascent antimicrobial stewardship efforts in low- and middle-income countries require setting-specific data. We aimed to evaluate risk factors for inpatient drug resistant Enterobacteriaceae colonization in a resource-limited setting in India. METHODS: Patients age ≥ 6 months admitted with ≥24 h of fever to a tertiary hospital in Pune, India were enrolled in a prospective cohort...
October 4, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/30285752/investigation-of-methicillin-resistant-staphylococcus-aureus-among-clinical-isolates-from-humans-and-animals-by-culture-methods-and-multiplex-pcr
#19
M M Rahman, K B Amin, S M M Rahman, A Khair, M Rahman, A Hossain, A K M A Rahman, M S Parvez, N Miura, M M Alam
BACKGROUND: Staphylococcus aureus is responsible for large numbers of hospital-related and community-acquired infections. In this study, we investigated the presence of S. aureus and methicillin-resistant S. aureus (MRSA) in 100 samples from animals (55 cattle, 36 dogs, and 9 cats) and 150 samples from hospitalized human patients. The samples were collected from healthy and diseased animals and from diseased humans and included milk, wound swab, pus, exudates, nasal swab and diabetic ulcer...
October 3, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30282566/whole-genome-sequencing-for-methicillin-resistant-staphylococcus-aureus-mrsa-outbreak-investigation-in-a-neonatal-intensive-care-unit
#20
Theresa Madigan, Scott A Cunningham, Robin Patel, Kerryl E Greenwood-Quaintance, Jean E Barth, Priya Sampathkumar, Nicolynn C Cole, Peggy C Kohner, Christopher E Colby, Garth F Asay, Jennifer L Fang, Christine A Baker, Angela L Heinrich, Kelly A Fjerstad, Maria J Lujero, Nicholas Chia, Patricio R Jeraldo, Heidi Nelson, W Charles Huskins
OBJECTIVE: To evaluate whole-genome sequencing (WGS) as a molecular typing tool for MRSA outbreak investigation. DESIGN: Investigation of MRSA colonization/infection in a neonatal intensive care unit (NICU) over 3 years (2014-2017). SETTING: Single-center level IV NICU.PatientsNICU infants and healthcare workers (HCWs). METHODS: Infants were screened for MRSA using a swab of the anterior nares, axilla, and groin, initially by targeted (ring) screening, and later by universal weekly screening...
October 4, 2018: Infection Control and Hospital Epidemiology
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