Alexander C Bashore, Hanying Yan, Chenyi Xue, Lucie Y Zhu, Eunyoung Kim, Thomas Mawson, Johana Coronel, Allen Chung, Nadja Sachs, Sebastian Ho, Leila S Ross, Michael Kissner, Emmanuelle Passegué, Robert C Bauer, Lars Maegdefessel, Mingyao Li, Muredach P Reilly
BACKGROUND: Atherosclerotic plaques are complex tissues composed of a heterogeneous mixture of cells. However, our understanding of the comprehensive transcriptional and phenotypic landscape of the cells within these lesions is limited. METHODS: To characterize the landscape of human carotid atherosclerosis in greater detail, we combined cellular indexing of transcriptomes and epitopes by sequencing and single-cell RNA sequencing to classify all cell types within lesions (n=21; 13 symptomatic) to achieve a comprehensive multimodal understanding of the cellular identities of atherosclerosis and their association with clinical pathophysiology...
February 22, 2024: Arteriosclerosis, Thrombosis, and Vascular Biology