Read by QxMD icon Read


Muslim Akmal, Tongku Nizwan Siregar, Sri Wahyuni, Hamny, Mustafa Kamal Nasution, Wiwik Indriati, Budianto Panjaitan, Dwinna Aliza
AIM: This study aims to determine the effect of seminal vesicle extract on cyclic adenosine monophosphate responsive element modulator (CREM) expression in rat Sertoli cells. MATERIALS AND METHODS: This study examined the expression of CREM on 20 male rats (Rattus norvegicus) at 4 months of age, weighing 250-300 g. The rats were divided into four groups: K0, KP1, KP2, and KP3. K0 group was injected with 0.2 ml normal saline; KP1 was injected with 25 mg cloprostenol (Prostavet C, Virbac S...
September 2016: Veterinary World
Nobuya Yoshida, Denis Comte, Masayuki Mizui, Kotaro Otomo, Florencia Rosetti, Tanya N Mayadas, José C Crispín, Sean J Bradley, Tomohiro Koga, Michihito Kono, Maria P Karampetsou, Vasileios C Kyttaris, Klaus Tenbrock, George C Tsokos
Inducible cAMP early repressor (ICER) has been described as a transcriptional repressor isoform of the cAMP response element modulator (CREM). Here we report that ICER is predominantly expressed in Th17 cells through the IL-6-STAT3 pathway and binds to the Il17a promoter, where it facilitates the accumulation of the canonical enhancer RORγt. In vitro differentiation from naive ICER/CREM-deficient CD4(+) T cells to Th17 cells is impaired but can be rescued by forced overexpression of ICER. Consistent with a role of Th17 cells in autoimmune and inflammatory diseases, ICER/CREM-deficient B6...
2016: Nature Communications
Jianhai Zhang, Fei Zheng, Chen Liang, Yuchen Zhu, Yan Shi, Yongli Han, Jundong Wang
Sulfur dioxide (SO2) is one of the main atmospheric pollutants worldwide, and is reported to be responsible for the formation of severe haze in China. Some studies have demonstrated a potential harmful effect of SO2 on the male reproductive system; however the underlying mechanism is still unknown. The purpose of this study is to investigate the roles of cytochrome P450 (P450), cAMP-responsive element modulator (CREM), and activator of CREM (ACT) in SO2-induced toxicity. Forty-eight male Wistar rats were randomly divided into an experimental and control group...
September 4, 2016: Environmental Toxicology and Pharmacology
Xiwen Lin, Miao Han, Lu Cheng, Jian Chen, Zhuqiang Zhang, Ting Shen, Min Wang, Bo Wen, Ting Ni, Chunsheng Han
Among all tissues of the metazoa, the transcritpome of testis displays the highest diversity and specificity. However, its composition and dynamics during spermatogenesis have not been fully understood. Here, we have identified 20,639 message RNAs (mRNAs), 7,168 long non-coding RNAs (lncRNAs) and 15,101 circular RNAs (circRNAs) in mouse spermatogenic cells, and found many of them were specifically expressed in testes. lncRNAs are significantly more testis-specific than mRNAs. At all stages, mRNAs are generally more abundant than lncRNAs, and linear transcripts are more abundant than circRNAs...
August 25, 2016: RNA Biology
Richard J Lewis, Marco Inserra, Irina Vetter, William C Holland, D Ransom Hardison, Patricia A Tester, R Wayne Litaker
BACKGROUND: Ciguatera is a circumtropical disease produced by polyether sodium channel toxins (ciguatoxins) that enter the marine food chain and accumulate in otherwise edible fish. Ciguatoxins, as well as potent water-soluble polyethers known as maitotoxins, are produced by certain dinoflagellate species in the genus Gambierdiscus and Fukuyoa spp. in the Pacific but little is known of the potential of related Caribbean species to produce these toxins. METHODS: We established a simplified procedure for extracting polyether toxins from Gambierdiscus and Fukuyoa spp...
2016: PloS One
Chieh-Ying Wang, Ming-Chu Tang, Wen-Chi Chang, Kenryo Furushima, Chuan-Wei Jang, Richard R Behringer, Chun-Ming Chen
Bobby sox homolog (Bbx) is an evolutionally conserved gene, but its biological function remains elusive. Here, we characterized defects of Bbx mutant rats that were created by PiggyBac-mediated insertional mutagenesis. Smaller body size and male infertility were the two major phenotypes of homozygous Bbx mutants. Bbx expression profile analysis showed that Bbx was more highly expressed in the testis and pituitary gland than in other organs. Histology and hormonal gene expression analysis of control and Bbx-null pituitary glands showed that loss of Bbx appeared to be dispensable for pituitary histogenesis and the expression of major hormones...
September 2016: Biology of Reproduction
A Reda, M Hou, T R Winton, R E Chapin, O Söder, J-B Stukenborg
STUDY QUESTION: Do the organ culture conditions, previously defined for in vitro murine male germ cell differentiation, also result in differentiation of rat spermatogonia into post-meiotic germ cells exhibiting specific markers for haploid germ cells? SUMMARY ANSWER: We demonstrated the differentiation of rat spermatogonia into post-meiotic cells in vitro, with emphasis on exhibiting, protein markers described for round spermatids. WHAT IS KNOWN ALREADY: Full spermatogenesis in vitro from immature germ cells using an organ culture technique in mice was first reported 5 years ago...
September 2016: Molecular Human Reproduction
Wonnam Kim, Do Rim Kim, Mun Seog Chang, Seong Kyu Park
Astragalus membranaceus BUNGE (AM; huáng qí) has been widely used as a medicinal herb for different kinds of diseases. AM treatment in vitro enhance sperm motility and ameliorates testicular toxicity, it has demonstrated the ability as a potential treatment for male infertility. In order to gain further insights on the molecular understanding of how AM enhances spermatogenesis, this study investigated whether AM has an affect on sperm parameters associated with cAMP response element modulator (CREM) and activator of CREM in testis (ACT) expression...
July 2016: Journal of Traditional and Complementary Medicine
Margaux Herpol, Katrien Lanckmans, Stefaan Van Neyghem, Pascale Clement, Stefanie Crevits, Kim De Crem, Frans K Gorus, Ilse Weets
OBJECTIVES: We evaluated the Bio-Rad (Irvine, CA) D-100 and the Sebia (Lisses, France) Capillarys 3 Tera for the measurement of hemoglobin A1c (HbA1c) in venous blood samples. METHODS: Whole-blood samples and control material were analyzed with the D-100 and Capillarys 3 Tera and compared with our routine method, HLC-723G7 (Tosoh, Tokyo, Japan). An evaluation protocol to test precision, trueness, linearity, carryover, and selectivity was set up according to Clinical and Laboratory Standards Institute guidelines...
July 2016: American Journal of Clinical Pathology
Kariane M Nunes, Cristian C C Teixeira, Renata C K Kaminski, Victor H V Sarmento, Renê O Couto, Sandra H Pulcinelli, Osvaldo Freitas
This article reports the development of a precursor liquid crystalline system based on a mixture of monoglycerides (MO) and Cremophor(®) (CREM) that exhibits in situ gelation to a liquid crystalline phase. The effects of different MO/CREM ratios and the water content (WC) on several performance characteristics were investigated with a full factorial design. The formulations were characterized by polarized light microscopy, small-angle X-ray scattering, and water uptake assays. Rheological, syringeability, and mucoadhesion evaluation were also performed...
August 2016: Journal of Pharmaceutical Sciences
Jure Ačimovič, Sandeep Goyal, Rok Košir, Marko Goličnik, Martina Perše, Ales Belič, Žiga Urlep, F Peter Guengerich, Damjana Rozman
Cholesterol synthesis is among the oldest metabolic pathways, consisting of the Bloch and Kandutch-Russell branches. Following lanosterol, sterols of both branches are proposed to be dedicated to cholesterol. We challenge this dogma by mathematical modeling and with experimental evidence. It was not possible to explain the sterol profile of testis in cAMP responsive element modulator tau (Crem τ) knockout mice with mathematical models based on textbook pathways of cholesterol synthesis. Our model differs in the inclusion of virtual sterol metabolizing enzymes branching from the pathway...
2016: Scientific Reports
Ewa Kozela, Ana Juknat, Fuying Gao, Nathali Kaushansky, Giovanni Coppola, Zvi Vogel
BACKGROUND: Our previous studies showed that the non-psychoactive cannabinoid, cannabidiol (CBD), ameliorates the clinical symptoms in mouse myelin oligodendrocyte glycoprotein (MOG)35-55-induced experimental autoimmune encephalomyelitis model of multiple sclerosis (MS) as well as decreases the memory MOG35-55-specific T cell (TMOG) proliferation and cytokine secretion including IL-17, a key autoimmune factor. The mechanisms of these activities are currently poorly understood. METHODS: Herein, using microarray-based gene expression profiling, we describe gene networks and intracellular pathways involved in CBD-induced suppression of these activated memory TMOG cells...
2016: Journal of Neuroinflammation
Jianhai Zhang, Yuchen Zhu, Chen Liang, Mingli Qie, Ruiyan Niu, Zilong Sun, Jinming Wang, Jundong Wang
Fluoride (F) is an essential trace element that humans and animals ingest from water, air, and fluoride-containing products; however, excessive fluoride absorption can damage a variety of organs and tissues, including the male reproductive system. Our previous studies found that fluoride exposure lowered sperm quality and interfered with spermatogenesis; however, the exact mechanism remained unclear. Proteins cytochrome P450 (P450), cAMP-responsive element modulator (CREM), and activator of CREM in testis (ACT) play the key roles in spermatogenesis and sperm motility...
May 27, 2016: Biological Trace Element Research
N Antony, A R McDougall, T Mantamadiotis, T J Cole, A D Bird
During mammalian lung development, the morphological transition from respiratory tree branching morphogenesis to a predominantly saccular architecture, capable of air-breathing at birth, is dependent on physical forces as well as molecular signaling by a range of transcription factors including the cAMP response element binding protein 1 (Creb1). Creb1(-/-) mutant mice exhibit complete neonatal lethality consistent with a lack of lung maturation beyond the branching phase. To further define its role in the developing mouse lung, we deleted Creb1 separately in the respiratory epithelium and mesenchyme...
2016: Scientific Reports
Yangyong Sun, Cheng Wang, Yinghui Xue, Qin Zhang, Rafael G Mendes, Linfeng Chen, Tao Zhang, Thomas Gemming, Mark H Rümmeli, Xinping Ai, Lei Fu
Conversion reaction electrode materials (CREMs) have gained significant interest in lithium-ion batteries (LIBs) owing to their high theoretical gravimetric capacity. However, traditional CREMs-based electrodes, with large strain arising from Li(+) intercalation/deintercalation causes pulverization or electrical breakdown and cracking of the active materials which leads to structural collapse, limiting performance. Therefore, in order to construct electrodes with a strong tolerance to the strain incurred during the conversion reaction process, we design a coral-like three-dimensional (3D) hierarchical heterostructure by using cross-linked nanoflakes interspersed with nanoparticles (NPs) standing vertically on graphene foam (GF)...
April 13, 2016: ACS Applied Materials & Interfaces
Guolong Fu, Youheng Wei, Xiaoli Wang, Long Yu
We aimed to investigate the internal existence status of testis-specific serine/threonine kinase 4 (Tssk4) and the interaction of Tssk4 and Cre-responsive element modulator (Crem). The internal existence status of Tssk4 in testis of mice was detected using western blotting and dephosphorylation method. The interaction of Tssk4 and Crem was analyzed by western blotting, immunohistochemistry, immunofluorescence, in vitro co-immunoprecipitation assays, and in vitro kinase assay. The results revealed that Tssk4 existed in testis both in phosphorylation and unphosphorylation status by a temporal manner with the development of testis...
June 2016: Bioscience, Biotechnology, and Biochemistry
May Al-Maghrebi, Waleed M Renno, Hoda F Al-Somali, Marina S Botras, Iman N Qadhi
Testicular ischemia reperfusion injury (tIRI) is considered as the underlying mechanism of testicular torsion, which can cause male infertility. tIRI-induced damage was investigated by assessing the gene expression of spermatogenesis master transcription factors (TFs) and that of major adhesion molecules of the blood-testis barrier. The effect of lutein, a hydroxyl carotenoid, in alleviating tIRI-induced damage was also studied. Male Sprague-Dawley rats were divided into three groups: sham, unilateral tIRI, and tIRI + lutein (0...
May 2016: Naunyn-Schmiedeberg's Archives of Pharmacology
Yu Feng, Jijing Tian, Irina Krylova, Tuan Xu, Heidi Qunhui Xie, Tai L Guo, Bin Zhao
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) exposure in humans is associated with marked immune suppressions and increased incidence of lymphoblastic diseases. To elucidate mechanisms of impairments in humoral immune responses, we used a murine model. Following a 20-week administration of low doses of TCDD, we observed severely reduced antibody titers, dramatically decreased number of splenic Th1 and Th2 cells and an increase in CD19(+) B cells. Transcriptional profiling of CD19(+) B cells showed that markers of pre-B cells were significantly elevated, indicating delayed B cell maturation...
January 2016: Journal of Environmental Sciences (China)
Diego A Forero, Liesbet Herteleer, Sonia De Zutter, Karl-Fredrik Norrback, Lars-Göran Nilsson, Rolf Adolfsson, Patrick Callaerts, Jurgen Del-Favero
Identification of novel candidate genes for schizophrenia (SZ) and bipolar disorder (BP), two psychiatric disorders with large epidemiological impacts, is a key research area in neurosciences and psychiatric genetics. Previous evidence from genome-wide studies suggests an important role for genes involved in synaptic plasticity in the risk for SZ and BP. We used a convergent genomics approach, combining different lines of biological evidence, to identify genes involved in the cAMP/PKA/CREB functional pathway that could be novel candidates for BP and SZ: CREB1, CREM, GRIN2C, NPY2R, NF1, PPP3CB and PRKAR1A...
April 2016: Schizophrenia Research
J S Schulte, E Fehrmann, M A Tekook, D Kranick, B Fels, N Li, X H T Wehrens, A Heinick, M D Seidl, W Schmitz, F U Müller
Chronic β-adrenergic stimulation is regarded as a pivotal step in the progression of heart failure which is associated with a high risk for arrhythmia. The cAMP-dependent transcription factors cAMP-responsive element binding protein (CREB) and cAMP-responsive element modulator (CREM) mediate transcriptional regulation in response to β-adrenergic stimulation and CREM repressor isoforms are induced after stimulation of the β-adrenoceptor. Here, we investigate whether CREM repressors contribute to the arrhythmogenic remodeling in the heart by analyzing arrhythmogenic alterations in ventricular cardiomyocytes (VCMs) from mice with transgenic expression of the CREM repressor isoform CREM-IbΔC-X (TG)...
March 2016: Basic Research in Cardiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"