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Oyeyemi O Ajayi, Sunday O Peters, Marcos De Donato, Sunday O Sowande, Fidalis D N Mujibi, Olanrewaju B Morenikeji, Bolaji N Thomas, Matthew A Adeleke, Ikhide G Imumorin
Background: Heat shock proteins (HSPs) are molecular chaperones known to bind and sequester client proteins under stress. Methods: To identify and better understand some of these proteins, we carried out a computational genome-wide survey of the bovine genome. For this, HSP sequences from each subfamily (sHSP, HSP40, HSP70 and HSP90) were used to search the Pfam (Protein family) database, for identifying exact HSP domain sequences based on the hidden Markov model. ProtParam tool was used to compute potential physico-chemical parameters detectable from a protein sequence...
2018: F1000Research
Yovana Cabrera, Leire Dublang, José Angel Fernández-Higuero, David Albesa-Jové, Maria Lucas, Ana Rosa Viguera, Marcelo E Guerin, Jose M G Vilar, Arturo Muga, Fernando Moro
Protein aggregate reactivation in metazoans is accomplished by the combined activity of Hsp70, Hsp40 and Hsp110 chaperones. Hsp110s support the refolding of aggregated polypeptides acting as specialized nucleotide exchange factors of Hsp70. We have studied how Apg2, one of the three human Hsp110s, regulates the activity of Hsc70 (HspA8), the constitutive Hsp70 in our cells. Apg2 shows a biphasic behavior: at low concentration, it stimulates the ATPase cycle of Hsc70, binding of the chaperone to protein aggregates and the refolding activity of the system, while it inhibits these three processes at high concentration...
December 3, 2018: Journal of Molecular Biology
Mohanned Naif Alhussien, Ajay Kumar Dang
The present study was undertaken to compare the effect of different seasons on the mammary immunity of three Indian native breeds of cows (Tharparkar, Gir and Sahiwal) well adapted to the tropical region. For this milk samples were collected from cows in winter (THI=57, comfortable zone), hot-dry (HD; THI=76, heat stressful zone) and hot-humid (HH; THI = 82, severe heat stress) and estimated for milk somatic cell counts (SCC), phagocytic activity (PA) of milk neutrophils, milk cortisol and heat shock proteins and function associated genes in milk neutrophils...
December 2018: Journal of Thermal Biology
Haidong Wei, Runxiang Zhang, Yingying Su, Yanju Bi, Xiang Li, Xin Zhang, Jianhong Li, Jun Bao
To investigate the effects of acute cold stress (ACS) on chicken heart after cold stimulation, female broilers were raised in either normal (C) or gradually decreasing temperatures (CS I and CS II) for 34 days followed by a 24 h ACS at 7°C. Cardiac tissues were collected from the pre-ACS and ACS time points to analyze the histopathological changes, antioxidant status and the expression of heat shock proteins, inflammatory factors and immune-related cytokines. The CS II heart tissues showed shrunken cell membranes and nuclei, disordered or ruptured myocardial fibers, higher MDA content and upregulation in HSP27, HSP40, HSP70, NF-κB, COX-2, PTGEs, iNOS, TNF-α and IL-4 mRNAs, and in protein levels of HSP40, NF-κB and iNOS and reduction in CAT, GSH-px and SOD activity, as well as HSP90 and IFN-γ levels compared to the control tissues before ACS...
2018: Frontiers in Physiology
Jagoda Mantej, Kinga Polasik, Ewa Piotrowska, Stefan Tukaj
Heat shock proteins (HSP) have been reported to impact immune responses and to be associated with rheumatoid arthritis (RA). Recently, we provided evidence for a role of autoantibodies to Hsp40 in patients with RA. In this study, we aimed at investigating the humoral autoimmune response to Hsp60, Hsp70, and Hsp90 in RA patients (n = 39). In comparison with healthy controls (n = 40), circulating IgG, IgM, and IgA autoantibodies against Hsp60, Hsp70, and Hsp90 were significantly increased in RA patients...
November 21, 2018: Cell Stress & Chaperones
Filip Trcka, Michal Durech, Pavla Vankova, Josef Chmelik, Veronika Martinkova, Jiri Hausner, Alan Kadek, Julien Marcoux, Tomas Klumpler, Borivoj Vojtesek, Petr Muller, Petr Man
Eukaryotic protein homeostasis (proteostasis) is largely dependent on the action of highly conserved Hsp70 molecular chaperones. Recent evidence indicates that, apart from conserved molecular allostery, Hsp70 proteins have retained and adapted the ability to assemble as functionally relevant ATP-bound dimers throughout evolution. Here, we have compared the ATP-dependent dimerization of DnaK, human stress-inducible Hsp70, Hsc70 and BiP Hsp70 proteins, showing that their dimerization propensities differ, with stress-inducible Hsp70 being predominantly dimeric in the presence of ATP...
November 20, 2018: Molecular & Cellular Proteomics: MCP
Ana O Tiroli-Cepeda, Thiago V Seraphim, Glaucia M S Pinheiro, Denio E P Souto, Lauro T Kubota, Júlio C Borges, Leandro R S Barbosa, Carlos H I Ramos
DnaJ/Hsp40 chaperones deliver unfolded proteins and stimulate the ATPase activity of DnaK/Hsp70 via their J-domain. However, the interaction is transient, creating a challenge for detailed analysis. We investigated whether it would be possible to gain further understanding of this interaction by engineering a chimeric polypeptide where the J-domain of Hsp40 was covalently attached to the substrate binding domain (SBD) of Hsp70 by a flexible linker. The rationale is to increase the proximity between the interacting partners to promote their natural interaction and facilitate the characterization of the interaction...
November 17, 2018: International Journal of Biological Macromolecules
Xiaoyan Zhu, Emiliya Poghosyan, Lenka Rezabkova, Bridget Mehall, Hitoshi Sakakibara, Masafumi Hirono, Ritsu Kamiya, Takashi Ishikawa, Pinfen Yang
HSP40s are regarded as co-chaperones perpetually shuttling client polypeptides to HSP70s for refolding. However, many HSP40s central for disparate processes diverge from this paradigm. To elucidate the noncanonical mechanisms, we investigated the HSP40 in the radial spoke (RS) complex in flagella. Disruption of the gene by the MRC1 transposon in Chlamydomonas resulted in jerky flagella. Traditional electron microscopy, cryo-electron tomography and sub-tomogram analysis revealed RSs of various altered morphologies which, unexpectedly, differed in the two RS species...
November 14, 2018: Molecular Biology of the Cell
Catherine A S Deane, Ian R Brown
HSPA6 (Hsp70B') is an inducible member of the Hsp70 (HSPA) family of heat shock proteins that is present in the human genome and not found in mouse and rat. Hence it is lacking in current animal models of neurodegenerative diseases. To advance knowledge of the little studied HSPA6, differentiated human neuronal SH-SY5Y cells were treated with the proteotoxic stress-inducing agent MG132. A robust induction of HSPA6 was apparent which localized to the periphery of MG132-induced protein aggregates in the neuronal cytoplasm...
2018: Journal of Alzheimer's Disease: JAD
Gang-Zheng Wang, Chao-Jun Ma, Yi Luo, Sha-Sha Zhou, Yan Zhou, Xiao-Long Ma, Ying-Li Cai, Jing-Jing Yu, Yin-Bing Bian, Yu-Hua Gong
BACKGROUND/AIMS: Heat stress could cause huge losses for Lentinula edodes in China and other Asian cultivation areas. Yet our understanding of mechanism how to defend to heat stress is incomplete. METHODS: Using heat-tolerant and heat-sensitive strains of L. edodes, we reported a combined proteome and transcriptome analysis of L. edodes response to 40 °C heat stress for 24 h. Meanwhile, the effect of LeDnaJ on the thermotolerance and IAA (indoleacetic acid) biosynthesis in L...
2018: Cellular Physiology and Biochemistry
C Wang, J Yang, Q Pan, S Yu, R Luo, H Liu, H Li, L Cong, C Ran
A stable reference gene is a key prerequisite for accurate assessment of gene expression. At present, the real-time reverse transcriptase quantitative polymerase chain reaction has been widely used in the analysis of gene expression in a variety of organisms. Neoseiulus barkeri Hughes (Acari: Phytoseiidae) is a major predator of mites on many important economically crops. Until now, however, there are no reports evaluating the stability of reference genes in this species. In view of this, we used GeNorm, NormFinder, BestKeeper, and RefFinder software tools to evaluate the expression stability of 11 candidate reference genes in developmental stages and under various abiotic stresses...
October 29, 2018: Bulletin of Entomological Research
Guilin Li, Hang Zhao, Xuemei Zhang, Yanming Zhang, Huayu Zhao, Xinxin Yang, Xingqi Guo, Baohua Xu
DnaJ, also known as Hsp40, plays important roles in maintaining the normal physiological state of an organism under stress conditions by mediating essential processes, such as protein synthesis, degradation, folding and metabolism. However, the exact functions of most DnaJ members are not fully understood in insects. Here, we identified three genes, AccDnaJA1 , AccDnaJB12 , and AccDnaJC8 , in Apis cerana cerana and explored their connection with the environmental stress response. Quantitative real-time PCR results showed that the mRNA levels of AccDnaJA1 , AccDnaJB12 , and AccDnaJC8 were all induced under cold, UV, H2 O2 and different pesticides treatment...
2018: Frontiers in Genetics
Guiping Gong, Luo Liu, Xu Zhang, Tianwei Tan
Oxidative stress is induced in many organisms by various natural abiotic factors including irradiation. It has been demonstrated that it significantly improves growth rate and lipid production of Rhodotorula glutinis. However, the specific mechanism of how irradiation influences the metabolism of R. glutinis remains still unavailable. To investigate and better understand the mechanisms involved in irradiation-induced stress resistance in R. glutinis, a multi-omics metabolism analysis was implemented. The results confirmed that irradiation indeed not only improved cell biomass but also accelerated the production of carotenoids and lipids, especially neutral lipid...
October 20, 2018: Applied Microbiology and Biotechnology
Hui Wang, Xue-Nan Li, Peng-Cheng Li, Wei Liu, Zheng-Hai Du, Jin-Long Li
Di(2-ethylhexyl) phthalate (DEHP) is an omnipresent environmental pollutant with endocrine disrupting properties. As a plasticizer, DEHP can be leach from the plastic to transfer the external environment and thus enters the animal food chain, causing serious damage to the animal organs. The heat-shock response (HSR) comprising heat-shock protein (HSPs) and heat-shock transcription factor (HSFs) plays a pivotal role in various toxic stress conditions. For the sake of investigating the effects of DEHP exposure on cardiac toxicity and the regulation of HSR, male quail were fed the diet with 0, 250, 500 and 750 mg/kg DEHP by gavage administration for 45 days...
October 2, 2018: Chemosphere
Brittany M Berger, June H Kim, Emily R Hildebrandt, Ian C Davis, Michael C Morgan, James L Hougland, Walter K Schmidt
Protein isoprenylation targets a subset of COOH-terminal Cxxx tetrapeptide sequences that has been operationally defined as a CaaX motif. The specificity of the farnesyl transferase toward each of the possible 8000 combinations of Cxxx sequences, however, remains largely unresolved. In part, it has been difficult to consolidate results stemming from in vitro and in silico approaches that yield a wider array of prenylatable sequences relative to those known in vivo We have investigated whether this disconnect results from the multi-step complexity of post-translational modification that occurs in vivo to CaaX proteins...
September 26, 2018: Genetics
Weibin Gong, Wanhui Hu, Linan Xu, Huiwen Wu, Si Wu, Hong Zhang, Jinfeng Wang, Gary W Jones, Sarah Perrett
The allosteric coupling of the highly conserved nucleotide- and substrate-binding domains of Hsp70 has been studied intensively. In contrast, the role of the disordered, highly variable C-terminal region of Hsp70 remains unclear. In many eukaryotic Hsp70s, the extreme C-terminal EEVD motif binds to the tetratricopeptide-repeat domains of Hsp70 co-chaperones. Here, we discovered that the TVEEVD sequence of Saccharomyces cerevisiae cytoplasmic Hsp70 (Ssa1) functions as a SUMO-interacting motif. A second C-terminal motif of ∼15 amino acids between the α-helical lid and the extreme C terminus, previously identified in bacterial and eukaryotic organellar Hsp70s, is known to enhance chaperone function by transiently interacting with folding clients...
November 16, 2018: Journal of Biological Chemistry
Lars Sjögren, Maïna Floris, Andrea Barghetti, Franziska Völlmy, Rune Linding, Peter Brodersen
ARGONAUTE1 (AGO1) binds directly to small regulatory RNA and is a key effector protein of post-transcriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA) in Arabidopsis The formation of an RNA-induced silencing complex (RISC) of AGO1 and small RNA requires the function of the heat shock protein 70/90 chaperone system. Some functions of AGO1 occur in association with endomembranes, in particular the rough endoplasmic reticulum (RER), but proteins interacting with AGO1 in membrane fractions remain unidentified...
October 26, 2018: Journal of Biological Chemistry
Huafeng Xu
The heat shock protein 70 (Hsp70) chaperones, vital to the proper folding of proteins inside cells, consume ATP and require cochaperones in assisting protein folding. It is unclear whether Hsp70 can utilize the free energy from ATP hydrolysis to fold a protein into a native state that is thermodynamically unstable in the chaperone-free equilibrium. Here I present a model of Hsp70-mediated protein folding, which predicts that Hsp70, as a result of differential stimulation of ATP hydrolysis by its Hsp40 cochaperone, dissociates faster from a substrate in fold-competent conformations than from one in misfolding-prone conformations, thus elevating the native concentration above and suppressing the misfolded concentration below their respective equilibrium values...
September 4, 2018: Scientific Reports
Nathan M Rowarth, Thomas H MacRae
Embryos of the crustacean, Artemia franciscana , develop either ovoviviparously or oviparously, respectively yielding swimming larvae (nauplii) or encysted gastrulae (cysts). Nauplii molt several times and become adults whereas cysts enter diapause, a state of dormancy characterized by exceptionally low metabolism and high stress tolerance. Synthesis of molecular chaperones such as the J-domain proteins, ArHsp40 and ArHsp40-2 occurs during embryo development and post-diapause growth of A. franciscana and they influence development and stress tolerance...
August 29, 2018: Journal of Experimental Biology
Preethi T Cherian, Irina Al-Khairi, Devarajan Sriraman, Ahmad Al-Enezi, Dalal Al-Sultan, Mohammed AlOtaibi, Saad Al-Enezi, Jaakko Tuomilehto, Fahd Al-Mulla, Jehad A Abubaker, Mohamed Abu-Farha
Heat shock response is an essential cellular stress response. Dysregulation of various heat shock proteins (HSPs), within the heat shock response (HSR) pathway, play a vital role in this host-defense mechanism contributing to obesity-induced insulin resistance and type 2 diabetes (T2D). Previously, we have reported changes in the expression levels of several HSPs such as HSP40, HSP60, HSP70, and HSP90 in obese compared with lean individuals. DNAJC27 is a member of the HSP40 protein family that was previously identified as a body mass index (BMI) associated locus in genome-wide association (GWAS) studies...
2018: Frontiers in Endocrinology
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