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trophoblast invasion

Hélène Clabault, Marie Cohen, Cathy Vaillancourt, J Thomas Sanderson
INTRODUCTION: Between 2 and 10% of pregnant women are treated with selective serotonin-reuptake inhibitors (SSRIs) for depression. The extravillous trophoblasts (evTBs), which migrate and invade maternal tissues, are crucial for embryo implantation and remodeling of maternal spiral arteries. Poor migration/invasion of evTBs can cause serious pregnancy complications, yet the effects of SSRIs on these processes has never been studied. To determine the effects of five SSRIs (fluoxetine, norfluoxetine, citalopram, sertraline and venlafaxine) on migration/invasion, we used JEG-3 and HIPEC cells as evTB models...
December 2018: Placenta
Daniele Di Mascio, Giuseppe Calì, Francesco D'antonio
Placenta accreta spectrum (PAS) disorders is a multifactorial process that encompasses a heterogeneous group of conditions characterized by an abnormal invasion of trophoblastic tissue through the myometrium and uterine serosa. PAS is associated with a high burden of adverse maternal outcomes including severe life-threatening hemorrhage, need for blood transfusion, damage to adjacent organs, and death. Prenatal screening of PAS is mandatory so that women may be counselled about the severity of this condition to plan management with a multidisciplinary team and delivery in a specialized center...
November 27, 2018: Minerva Ginecologica
Jürgen Pollheimer, Sigrid Vondra, Jennet Baltayeva, Alexander Guillermo Beristain, Martin Knöfler
During placentation invasive extravillous trophoblasts (EVTs) migrate into the maternal uterus and modify its vessels. In particular, remodeling of the spiral arteries by EVTs is critical for adapting blood flow and nutrient transport to the developing fetus. Failures in this process have been noticed in different pregnancy complications such as preeclampsia, intrauterine growth restriction, stillbirth, or recurrent abortion. Upon invasion into the decidua, the endometrium of pregnancy, EVTs encounter different maternal cell types such as decidual macrophages, uterine NK (uNK) cells and stromal cells expressing a plethora of growth factors and cytokines...
2018: Frontiers in Immunology
Feixia Gao, Chun Zhou, Weiyu Qiu, Haiwang Wu, Jing Li, Jinting Peng, Min Qiu, Chun Liang, Jie Gao, Songping Luo
Miscarriage is a common condition during pregnancy and its mechanisms remain largely unknown. Extravillous trophoblast (EVT) cell invasion is required to maintain normal pregnancy and its malfunction has been proposed as a major cause for miscarriage. Homeostasis of matrix metalloproteinase 9 (MMP9) is a key to regulate EVT cell invasion. Total flavonoids from Semen Cuscutae (TFSC) have been applied clinically used for preventing or treating miscarriage in the past. Given its potential clinical benefit on preventing miscarriage, this study aims at examining the therapeutic effect of TFSC in the prevention of premature birth by upregulating MMP9 and promote EVT cell invasion...
November 26, 2018: Scientific Reports
Nunzia Zanotta, Lorenzo Monasta, Kristina Skerk, Stefania Luppi, Monica Martinelli, Giuseppe Ricci, Manola Comar
PROBLEM: Cytokines have a significant role in the process of embryo implantation, trophoblast growth, and differentiation by modulating the immune and endocrine system. The aim of this study was to investigate the profile of a large set of cytokines in the cervico-vaginal washing of women undergoing IVF, to explore the association of these proteins with a good receptive endometrium. METHOD OF STUDY: A cohort of 155 women scheduled for IVF cycle was recruited. All patients were asymptomatic for genitourinary infections and had been screened for chlamydia, mycoplasma, and other bacterial infections...
November 26, 2018: American Journal of Reproductive Immunology: AJRI
Teena Kjb Gamage, William Schierding, Daniel Hurley, Peter Tsai, Jackie L Ludgate, Chandrakanth Bhoothpur, Lawrence W Chamley, Robert J Weeks, Erin C Macaulay, Joanna L James
The placenta is a vital fetal exchange organ connecting mother and baby. Specialised placental epithelial cells, called trophoblasts, are essential for adequate placental function. Trophoblasts transform the maternal vasculature to allow efficient blood flow to the placenta and facilitate adequate nutrient uptake. Placental development is in part regulated by epigenetic mechanisms. However, our understanding of how DNA methylation contributes to human trophoblast differentiation is limited. To better understand how genome-wide methylation differences affect trophoblast differentiation, reduced representation bisulfite sequencing (RRBS) was conducted on four matched sets of trophoblasts (a candidate human trophoblast stem cell population), cytotrophoblasts (an intermediate progenitor population) and extravillous trophoblasts (EVT, a terminally differentiated population) each isolated from the same first trimester placenta...
November 26, 2018: Epigenetics: Official Journal of the DNA Methylation Society
Ling Huang, Yuanyuan Li, Chunhui Wang, Na Li, Yue Hou, Ruijing Chang, Man Sun, Ruochen Wang, Lei Zhu, Chong Qiao
Collapsin response mediator protein 1 (CRMP-1) is widely expressed in the nervous system and has tumor suppressive effects. Our previous studies have demonstrated that CRMP-1 was expressed in the trophoblasts of the whole stage of pregnancy with significantly increasing expression in the placenta of early-onset preeclampsia. Preeclampsia, especially early onset, is strongly associated with the dysfunction of trophoblast including proliferation, apoptosis, migration, and invasion. In this study, we found an inhibitory effect of CRMP-1 on proliferation, migration, invasion, and an enhanced effect on apoptosis in human trophoblast cell lines HTR-8/SVneo and JEG-3 by MTT assay, colony formation assay, cell viability assay, caspase 3/7 activity assay, scratch wound assay, and Matrigel Transwell assay...
November 22, 2018: Reproductive Sciences
Qing Han, Lianghui Zheng, Zhaodong Liu, Jinying Luo, Rongxin Chen, Jianying Yan
OBJECTIVES: To investigate the expression of β-catenin in chorionic villi, and to explore its roles in placenta accreta and placenta previa. METHODS: We compared β-catenin expression in the control group, placenta accreta group (lesion area and normal zones), and placenta previa group (placental central and placental edge zones) by immunohistochemistry, Western blotting, and RT-PCR techniques. RESULTS: Compared with the normal group, the placenta accreta group had a longer length of stay, greater bleeding volume, and lower newborn birth weight...
November 22, 2018: Journal of International Medical Research
Sonia DaSilva-Arnold, Che-Ying Kuo, Viralkumar Davra, Yvonne Remache, Peter C W Kim, John P Fisher, Stacy Zamudio, Abdulla Al-Khan, Raymond B Birge, Nicholas P Illsley
STUDY QUESTION: Does the upregulation of the zinc finger E-box binding homeobox 2 (ZEB2) transcription factor in human trophoblast cells lead to alterations in gene expression consistent with an epithelial-mesenchymal transition (EMT) and a consequent increase in invasiveness? SUMMARY ANSWER: Overexpression of ZEB2 results in an epithelial-mesenchymal shift in gene expression accompanied by a substantial increase in invasive capacity of human trophoblast cells. WHAT IS KNOWN ALREADY: In-vivo results have shown that cytotrophoblast differentiation into extravillous trophoblast involves an epithelial-mesenchymal transition...
November 21, 2018: Molecular Human Reproduction
Jiamin Xie, Yuqing Xu, Liuxia Wan, Peng Wang, Miaomiao Wang, Minyue Dong
INTRODUCTION: Preeclampsia is a main cause of maternal and perinatal mortality and morbidity. The expression of follistatin-like 3 (FSTL3) is enhanced in maternal serum and placenta of preeclamptic women. However, whether FSTL3 is involved in the pathophysiologic of preeclampsia has not been clarified yet. METHOD: Trophoblast cell lines Swan71 and JAR cells were cultured and siRNA was used to silence FSTL3. The expression of FSTL3 was determined by Western blotting...
November 30, 2018: Biochemical and Biophysical Research Communications
Cheuk-Lun Lee, Jan H W Veerbeek, Tirtha K Rana, Bas B van Rijn, Graham J Burton, Hong Wa Yung
Shallow extravillous trophoblast (EVT) invasion is central to the pathophysiology of many pregnancy complications. Invasion is mediated partially by matrix metalloproteinases (MMPs). MMP-2 is highly expressed in early pregnancy. MMP activity can be regulated by pro-inflammatory cytokines, which also induce endoplasmic reticulum (ER) stress in other cells. We investigated whether pro-inflammatory cytokines regulate MMP-2 activity through ER stress response pathways in trophoblast, before exploring potential regulatory mechanisms...
November 15, 2018: American Journal of Pathology
Yinghong Zhang, Xianzhen Pan, Xiaoyan Yu, Lei Li, Hongmei Qu, Shuhong Li
BACKGROUND: The remodeling of maternal spiral artery following embryo implantation, which relies on well-regulated trophoblast functions, is a pivotal process to ensure a successful pregnancy. Low-density lipoprotein receptor-related protein 6 (LRP6) and microRNAs (miRNAs, miRs) are suggested to be involved in angiogenesis and several vascular diseases; however, their functions in the control of trophoblast remain elusive. We therefore aimed to examine the roles of LRP6 and miR-590-3p in the regulation of trophoblast during the remodeling of maternal spiral artery...
November 8, 2018: Molecular Genetics & Genomic Medicine
Sarah T K Sin, Yan Li, Ming Liu, Yun-Fei Yuan, Stephanie Ma, Xin-Yuan Guan
Hepatocellular carcinoma (HCC) is one of the most common and lethal cancer types worldwide, especially in Asian countries. Genetic alterations, including hyperactivation of oncogenes and loss of expression of tumor suppressor genes, greatly contribute to the initiation and progression of HCC. Here we report that down-regulation of trophoblast cell surface antigen 2 ( TROP-2 ) was frequently detected in HCC. Transcriptome sequencing of non-tumor and HCC patient samples revealed down-regulation of TROP-2 in tumor tissues...
November 2018: Hepatology Communications
Ting Zhong, Xingxing Xie, Teng Zong, Xiaochun Yu, Yan Ling, Haibin Kuang
Uterine natural killer (uNK) cells have been hypothesized to serve a role in controlling trophoblast invasion and proliferation. The aim of the present study was to identify the distribution and number of uNK cells in normal pregnancy (NP), partial mole (PM), complete mole (CM) and invasive mole (IM). uNK cells were detected using dolichos biflorus agglutinin lectin immunohistochemistry in decidual and villous tissues from early NP (n=15), late NP (n=15), PM (n=22), CM (n=20) and IM (n=10). A scaled eye piece was used for cell counting to obtain semi-quantitative results...
November 2018: Oncology Letters
Y-N Fang, Z-L Huang, H Li, W-B Tan, Q-G Zhang, L Wang, J-L Wu
OBJECTIVE: The role of miR-182-5p in preeclampsia was studied, and its mechanism was also explored. PATIENTS AND METHODS: Fifty patients with preeclampsia were assigned to the study group and 50 normal pregnant women to the control group. The age, weight, blood pressure, urinary protein, and weight of newborns were compared between the two groups. The placental tissues of the above-mentioned subjects were collected, and quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) assay was used to detect the expression of miR-182-5p...
October 2018: European Review for Medical and Pharmacological Sciences
Bethany Hart, Elizabeth Morgan, Emilyn Alejandro
Fetal growth restriction is one of the most common obstetrical complications resulting in significant perinatal morbidity and mortality. The most frequent etiology of human singleton fetal growth restriction is placental insufficiency, which occurs secondary to reduced utero-placental perfusion, abnormal placentation, impaired trophoblast invasion and spiral artery remodeling, resulting in altered nutrient and oxygen transport. Two nutrient-sensing proteins involved in placental development and glucose and amino acid transport are mechanistic target of rapamycin (mTOR) and O-linked N-acetylglucosamine transferase (OGT), which are both regulated by availability of oxygen...
October 1, 2018: Journal of Molecular Endocrinology
Perrine Ginod, Cécile Choux, Julie Barberet, Thierry Rousseau, Céline Bruno, Bouchra Khallouk, Paul Sagot, Karine Astruc, Patricia Fauque
OBJECTIVE: To study the impact of in vitro fertilization, with or without intracytoplasmic sperm injection (IVF/ICSI), frozen-embryo transfer (FET), and intrauterine insemination (IUI) on fetal growth kinetics throughout pregnancy and to compare the different modes of conception. DESIGN: Retrospective cohort study. SETTING: University. PATIENT(S): A total of 560 singleton pregnancies were included (96 IVF, 210 ICSI, 121 FET, and 133 IUI)...
November 2018: Fertility and Sterility
Masako Mizuno, Rika Miki, Yoshinori Moriyama, Takafumi Ushida, Kenji Imai, Kaoru Niimi, Tomoko Nakano, Hiroyuki Tsuda, Seiji Sumigama, Eiko Yamamoto, Takeshi Senga, Akira Iwase, Fumitaka Kikkawa, Tomomi Kotani
Recent studies have reported that E2F transcription factor (E2F) 8, an atypical E2F transcription factor, serves a critical role in promoting the growth and development of the murine placenta. However, the function of E2F8 in the human placenta remains unknown. Invasion of extravillous trophoblasts (EVTs) into the maternal decidua is known to be important for the development of the human placenta. To investigate the role of E2F8 in human placental development, E2F8 localisation was examined in the human placenta and E2F8 mRNA expression was detected in primary cultured EVTs...
November 1, 2018: Molecular Medicine Reports
Eiko Kawamura, Gina B Hamilton, Ewa I Miskiewicz, Daniel J MacPhee
BACKGROUND: Integrins are transmembrane receptors that mediate cell-extracellular matrix (ECM) and cell-cell adhesion and trophoblast cells undergo changes in integrin expression as they differentiate. However, the mechanism(s) of integrin activation leading to integrin-mediated signaling in trophoblast cell differentiation is unknown. The Fermitin family proteins are integrin activators that help mediate integrin-mediated signaling, but have never been studied in detail within the human placenta...
November 1, 2018: BMC Developmental Biology
Huisheng Ge, Nanlin Yin, Ting-Li Han, Dongni Huang, Xuehai Chen, Ping Xu, Chengjin He, Chao Tong, Hongbo Qi
Preeclampsia (PE) is a pregnancy-specific disorder representing a major cause of maternal and perinatal morbidity and mortality. Invasive and migratory phenotypes are acquired by trophoblasts through the process of epithelial-mesenchymal transition (EMT). Studies have shown that trophoblast EMT events are dysregulated in PE and play an important role in its development. Dysregulation of interleukin (IL)-27 and IL-27R (T-cell cytokine receptor (TCCR)/WSX -1) is relevant to PE. In this study, our results demonstrated that IL-27 did not significantly affect the proliferation and apoptosis of HTR -8/SVneo trophoblast cells, while it did significantly inhibit trophoblast invasion and migration...
October 29, 2018: Reproductive Sciences
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