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https://www.readbyqxmd.com/read/30280227/development-and-validation-of-an-lc-ms-ms-assay-for-the-quantification-of-dolutegravir-extracted-from-human-hair
#1
Craig Sykes, Kimberly Blake, Nicole White, Amanda P Schauer, Bryan B Guzman, Mackenzie L Cottrell, Bani Tamraz, Angela D M Kashuba
Measurement of drug concentrations in hair provides a non-invasive approach to assess drug adherence. Here, we report on the development and validation of a method for the quantification of the antiretroviral dolutegravir (DTG) extracted from human hair. DTG is extracted from hair samples by sonication and incubation in 50:50 methanol:acetonitrile with 2% formic acid overnight at 40 °C. Following extraction, samples are analyzed by reverse-phase chromatography on a Waters Atlantis T3 (50 × 2.1 mm, 3-μm particle size) column with subsequent detection by electrospray ionization in positive ion mode on an AB Sciex API-5000 triple quadrupole mass spectrometer...
October 2, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30248417/14-deoxy-11-12-didehydroandrographolide-suppresses-adipogenesis-of-3-t3-l1-preadipocytes-by-inhibiting-ccaat-enhancer-binding-protein-%C3%AE-activation-and-ampk-mediated-mitotic-clonal-expansion
#2
Chien-Chun Li, Chih-Ching Yen, Ciou-Ting Fan, Wei-Ting Chuang, Chin-Shiu Huang, Haw-Wen Chen, Chong-Kuei Lii
Obesity is highly correlated with several metabolic disorders. Adipocyte differentiation is a key process in determining obesogenesis. 14-Deoxy-11,12-didehydroandrographolide (deAND) is a diterpenoid rich in Andrographis paniculata (Burm.f.) Nees., a herbal medicine commonly used to treat colds, infections, and liver diseases. We investigated whether deAND inhibits the adipogenesis of 3T3-L1 cells and the underlying mechanisms. We found that deAND (0-15 μM) dose-dependently inhibits the mRNA and protein expression of peroxisome proliferator-activated receptor γ, sterol regulatory element-binding protein 1c, fatty acid synthase, and stearoyl-CoA desaturase-1...
September 21, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/30233497/a-renewed-focus-on-the-association-between-thyroid-hormones-and-lipid-metabolism
#3
REVIEW
Leonidas H Duntas, Gabriela Brenta
Thyroid dysfunction, manifesting as either overt or subclinical hypothyroidism, negatively affects lipid metabolism: this leads to hypercholesterolemia which progressively increases the risk for cardiovascular disease and, potentially, mortality. Hypercholesterolemia in hypothyroidism is mainly due to a reduction in low-density lipoprotein (LDL) receptor activity, this accompanied by concomitant diminishing control by triiodothyronine (T3) of sterol regulatory element-binding protein 2 (SREBP-2), which modulates cholesterol biosynthesis by regulating rate-limit degrading enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA) activity...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/30197625/improved-thyroid-hypoechogenicity-following-bariatric-induced-weight-loss-in-euthyroid-adults-with-severe-obesity-a-pilot-study
#4
Ioannis Kyrou, Olu Adesanya, Nicholas Hedley, Sarah Wayte, Dimitris Grammatopoulos, Claire L Thomas, Andrew Weedall, Subash Sivaraman, Lavanya Pelluri, Thomas M Barber, Vinod Menon, Harpal S Randeva, Miroslav Tedla, Martin O Weickert
Background: Obesity may affect both biochemical thyroid function tests; and thyroid morphology, as assessed using ultrasound scans (US). The aim of the present pilot study was to explore whether weight loss achieved by bariatric surgery alters thyroid US morphology including gray-scale measurements; and/or function in euthyroid adults with severe obesity. Methods: Euthyroid adults (>18 years) with body mass index (BMI) ≥40 kg/m2 and negative thyroid peroxidase antibodies were assessed at baseline (pre-surgery) and after achieving at least 5% weight loss of their baseline body weight following bariatric surgery...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/30177344/positive-and-negative-symptoms-in-schizophrenia-a-longitudinal-analysis-using-latent-variable-structural-equation-modelling
#5
Giuseppe Carrà, Cristina Crocamo, Matthias Angermeyer, Traolach Brugha, Mondher Toumi, Paul Bebbington
BACKGROUND: Recent network models of schizophrenia propose it is the consequence of mutual interaction between its symptoms. While cross-sectional associations between negative and positive symptoms are consistent with this idea, they may merely reflect their involvement in the diagnostic process. Longitudinal analyses however may allow the identification of possible causal relationships. The European Schizophrenia Cohort (EuroSC) provides data suitable for this purpose. METHODS: EuroSC includes 1208 patients randomly sampled from outpatient services in France, Germany and the UK...
August 31, 2018: Schizophrenia Research
https://www.readbyqxmd.com/read/30170956/dibutyl-phthalate-exposure-from-mesalamine-medications-and-serum-thyroid-hormones-in-men
#6
Feiby L Nassan, Tim I M Korevaar, Brent A Coull, Niels E Skakkebæk, Stephen A Krawetz, Molly Estill, Elizabeth J Hait, Joshua R Korzenik, Jennifer B Ford, Ralph A De Poortere, Maarten A Broeren, Alan C Moss, Thomas R Zoeller, Russ Hauser
BACKGROUND: Dibutyl phthalate (DBP) is an endocrine disruptor and used in some medication coatings, such as mesalamine for treatment inflammatory bowel disease (IBD). OBJECTIVES: To determine whether high-DBP from some mesalamine medications alters thyroid function. METHODS: Seventy men with IBD, without thyroid disease or any radiation history participated in a crossover-crossback prospective study and provided up to 6 serum samples (2:baseline, 2:crossover, 2:crossback)...
August 28, 2018: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/30136516/-determination-of-six-cephalosporin-residues-in-milk-by-quechers-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#7
Xiaogang Zhang, Xia Wang, Chunfeng Dai, Meilian Chen
A simple, rapid and sensitive method for the simultaneous determination of six cephalosporin (cephalexin, cephapirin, cefotaxime, cefazolin, cefalonium and cefquinome) residues in milk was developed by QuEChERS coupled with ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).The milk samples were extracted with 10 mL acetonitrile containing 1%(v/v) acetic acid, and then centrifuged for 10 min.The supernatant was cleaned up with 100 mg C18 adsorbent powder.The analytes were separated on a reversed phase HSS T3 column (100 mm×2...
April 8, 2018: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/30086211/a-novel-igsf1-mutation-in-a-large-irish-kindred-highlights-the-need-for-familial-screening-in-the-igsf1-deficiency-syndrome
#8
Edna F Roche, Anne McGowan, Olympia Koulouri, Marc-Olivier Turgeon, Adeline K Nicholas, Emmeline Heffernan, Ranna El-Khairi, Noina Abid, Greta Lyons, David Halsall, Marco Bonomi, Luca Persani, Mehul T Dattani, Mark Gurnell, Daniel J Bernard, Nadia Schoenmakers
OBJECTIVE: Loss-of-function mutations in IGSF1 result in X-linked central congenital hypothyroidism (CeCH), occurring in isolation or associated with additional pituitary hormone deficits. Intrafamilial penetrance is highly variable and a minority of heterozygous females are also affected. We identified and characterized a novel IGSF1 mutation and investigated its associated phenotypes in a large Irish kindred. DESIGN, PATIENTS AND MEASUREMENTS: A novel hemizygous IGSF1 mutation was identified by direct sequencing in two brothers with CeCH, and its functional consequences were characterized in vitro...
August 7, 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/30074252/development-of-a-method-for-the-determination-of-hydromorphone-in-plasma-by-lc-ms
#9
Sherry Cox, Joan Bergman, Shawna Hawkins, Kurt Sladky
A simple high-performance liquid chromatography method for the determination of hydromorphone in small volume plasma has been developed. Following solid-phase extraction using Oasis HLB cartridges, samples were separated by reverse-phase high-performance liquid chromatography on an Atlantis T3 4.6 × 150 mm column (3.0 μm) and quantified using mass spectrometry. The mobile phase was a mixture of water with 0.1% formic acid and acetonitrile with 0.1% formic acid (91:9). The standard curve ranged from 1 to 500 ng/mL...
August 3, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/30071781/enamel-resistance-to-demineralisation-around-orthodontic-brackets-after-co-2-laser-irradiation-a-randomised-clinical-trial
#10
Tuqa Raghis, Ghiath Mahmoud, Ahmed Abdullah, Omar Hamadah
OBJECTIVE: To evaluate the clinical effect of 10.6 µm CO2 laser irradiation on the formation of demineralised lesions (DLs) around orthodontic brackets. DESIGN: A two-arm, split-mouth, randomised clinical trial (RCT). SETTING: The Orthodontic Department, Faculty of Dentistry, Damascus University, Syria. PARTICIPANTS: Twenty-six patients with a total of 520 teeth were recruited into the trial. METHOD: Two reversed quadrants of the dental arches in each patient were randomly allocated to CO2 laser application around the orthodontic brackets...
August 2, 2018: Journal of Orthodontics
https://www.readbyqxmd.com/read/30007154/transcriptomics-investigation-of-thyroid-hormone-disruption-in-the-olfactory-system-of-the-rana-lithobates-catesbeiana-tadpole
#11
Kevin W Jackman, Nik Veldhoen, Rachel C Miliano, Bonnie J Robert, Linda Li, Azadeh Khojasteh, Xiaoyu Zheng, Tristan S M Zaborniak, Graham van Aggelen, Mary Lesperance, Wayne J Parker, Eric R Hall, Gregory G Pyle, Caren C Helbing
Thyroid hormones (THs) regulate vertebrate growth, development, and metabolism. Despite their importance, there is a need for effective detection of TH-disruption by endocrine disrupting chemicals (EDCs). The frog olfactory system substantially remodels during TH-dependent metamorphosis and the objective of the present study is to examine olfactory system gene expression for TH biomarkers that can evaluate the biological effects of complex mixtures such as municipal wastewater. We first examine classic TH-response gene transcripts using reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) in the olfactory epithelium (OE) and olfactory bulb (OB) of premetamorphic Rana (Lithobates) catesbeiana tadpoles after 48 h exposure to biologically-relevant concentrations of the THs, 3,5,3'-triiodothyronine (T3 ) and L-thyroxine (T4 ), or 17-beta estradiol (E2 ); a hormone that can crosstalk with THs...
September 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29953409/severe-consumptive-hypothyroidism-caused-by-multiple-infantile-hepatic-haemangiomas
#12
Enver Simsek, Meliha Demiral, Elif Gundoğdu
Background Infantile hepatic haemangiomas (IHHs) produce an excess of the thyroid hormone inactivating enzyme type-3 iodothyronine deiodinase (D3), leading to rapid degradation of thyroid hormones and consumptive hypothyroidism. The L-thyroxine replacement dose in patients with consumptive hypothyroidism is inappropriately higher than that in congenital hypothyroidism. Case presentation A 4-month-old boy presented with abdominal distention. Thyroid function tests (TFTs) revealed an elevated thyroid-stimulating hormone (TSH) level of 177 mU/L, normal free thyroxine (fT4) of 1...
July 26, 2018: Journal of Pediatric Endocrinology & Metabolism: JPEM
https://www.readbyqxmd.com/read/29920214/triiodothyronine-and-leptin-repletion-in-humans-similarly-reverse-weight-loss-induced-changes-in-skeletal-muscle
#13
Michael Rosenbaum, Rochelle L Goldsmith, Fadia Haddad, Kenneth M Baldwin, Richard Smiley, Dympna Gallagher, Rudolph L Leibel
Subjects maintaining a 10% or greater dietary weight loss exhibit decreased circulating concentrations of bioactive thyroid hormones and increased skeletal muscle work efficiency largely due to increased expression of more efficient myosin heavy chain isoforms (MHCI) and significantly mediated by the adipocyte-derived hormone leptin. The primary purpose of this study was to examine the effects of triiodothyronine (T3) repletion on energy homeostasis and skeletal muscle physiology in weight-reduced subjects and to compare these results with the effects of leptin repletion...
June 19, 2018: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29895574/primary-t-cells-from-cutaneous-t-cell-lymphoma-skin-explants-display-an-exhausted-immune-checkpoint-profile
#14
Christiane Querfeld, Samantha Leung, Patricia L Myskowski, Shane A Curran, Debra A Goldman, Glenn Heller, Xiwei Wu, Sung Hee Kil, Sneh Sharma, Kathleen J Finn, Steven Horwitz, Alison Moskowitz, Babak Mehrara, Steven T Rosen, Allan C Halpern, James W Young
Cutaneous T-cell lymphoma (CTCL) develops from clonally expanded CD4+ T cells in a background of chronic inflammation. Although dendritic cells (DCs) stimulate T cells and are present in skin, cutaneous T cells in CTCL do not respond with effective antitumor immunity. We evaluated primary T-cell and DC émigrés from epidermal and dermal explant cultures of skin biopsies from CTCL patients ( n = 37) and healthy donors ( n = 5). Compared with healthy skin, CD4+ CTCL populations contained more T cells expressing PD-1, CTLA-4, and LAG-3...
August 2018: Cancer Immunology Research
https://www.readbyqxmd.com/read/29892819/thyroid-hormones-and-derivatives-endogenous-thyroid-hormones-and-their-targets
#15
Josef Köhrle
More than a century after the discovery of L-Thyroxine, the main thyroid hormone secreted solely by the thyroid gland, several metabolites of this iodinated, tyrosine-derived ancestral hormone have been identified. These are utilized as hormones during development, differentiation, metamorphosis, and regulation of most biochemical reactions in vertebrates and their precursor species. Among those metabolites are the thyromimetically active 3,3',5-Triiodo-L-thyronine (T3) and 3,5-Diiodo-L-thronine, reverse-T3 (3,3',5'-Triiodo-L-thyronine) with still unclear function, the recently re-discovered thyronamines (e...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29890234/principles-and-practice-of-high-dose-rate-penile-brachytherapy-planning-and-delivery-techniques
#16
Shireen Parsai, Sheen Cherian, Ryan K Berglund, Byron Lee, Matthew Kolar, Nancy Nagle-Hernan, Allan Wilkinson, Jay Ciezki
PURPOSE: To allow for organ preservation, high-dose rate (HDR) brachytherapy may be used in the treatment of localized penile cancer. Penile cancer is a rare malignancy that accounts for <1% of cancers in men in the United States. The standard treatment for localized disease is partial amputation of the penis. However, patients with T1b or T2 disease <4 cm in maximum dimension and confined to the glans penis may be treated with brachytherapy as an organ-sparing approach. Previous works have described the technique involved for low-dose rate brachytherapy; however, we detail the techniques involved with HDR brachytherapy...
June 8, 2018: Practical Radiation Oncology
https://www.readbyqxmd.com/read/29874126/deficiency-of-type-2-iodothyronine-deiodinase-reduces-necroptosis-activity-and-oxidative-stress-responses-in-retinas-of-leber-congenital-amaurosis-model-mice
#17
Fan Yang, Hongwei Ma, Michael R Butler, Xi-Qin Ding
Thyroid hormone (TH) signaling has been shown to regulate cone photoreceptor viability. Suppression of TH signaling with antithyroid drug treatment or by targeting iodothyronine deiodinases and TH receptors preserves cones in mouse models of retinal degeneration, including the Leber congenital amaurosis Rpe65-deficient mice. This work investigates the cellular mechanisms underlying how suppressing TH signaling preserves cones in Rpe65-deficient mice, using mice deficient in type 2 iodothyronine deiodinase (Dio2), the enzyme that converts the prohormone thyroxine to the active hormone triiodothyronine (T3)...
June 6, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29846992/baseline-adverse-electrical-remodeling-and-the-risk-for-ventricular-arrhythmia-in-cardiac-resynchronization-therapy-recipients-madit-crt
#18
Yitschak Biton, Chee Yuan Ng, Xiaojuan Xia, Jayson R Baman, Jean-Philippe Couderc, Arthur J Moss, Valentina Kutyifa, Scott McNitt, Bronislava Polonsky, Wojciech Zareba
INTRODUCTION: Adverse electrical remodeling (AER), represented here as the sum absolute QRST integral (SAI QRST), has previously been shown to be directly associated with the risk for ventricular arrhythmia (VA). Cardiac resynchronization therapy (CRT) is known to reduce the risk for VA through various mechanisms, including reverse remodeling, and we aimed to evaluate the association between baseline AER and the risk for VA in CRT recipients. METHODS AND RESULTS: The study population comprised 961 CRT-D implanted patients from the MADIT CRT study...
July 2018: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/29846461/hemodynamic-evaluation-of-elderly-patients-during-laparoscopic-cholecystectomy
#19
Luiz Paulo Jacomelli Ramos, Rodrigo Barcellos Araújo, Maria DO Carmo Valente Castro, Maria Roberta Meneguetti Seravalli Ramos, José Antonio Cunha-E-Silva, Antonio Carlos Iglesias
OBJECTIVE: to demonstrate hemodynamic changes during laparoscopic cholecystectomy in elderly patients with trans-esophageal echocardiography. METHODS: we studied 31 elderly patients (aged 60 years or older), ASA I or II, who underwent elective laparoscopic cholecystectomy under general, standardized anesthesia, with cardiovascular parameters measured using transesophageal echocardiography at three different times: before the pneumoperitoneum (T1), after CO2 insufflation (T2) and at deflation (T3)...
2018: Revista do Colégio Brasileiro de Cirurgiões
https://www.readbyqxmd.com/read/29796989/thyroid-hormone-supplementation-restores-spatial-memory-hippocampal-markers-of-neuroinflammation-plasticity-related-signaling-molecules-and-%C3%AE-amyloid-peptide-load-in-hypothyroid-rats
#20
Amina Chaalal, Roseline Poirier, David Blum, Serge Laroche, Valérie Enderlin
Hypothyroidism is a condition that becomes more prevalent with age. Patients with untreated hypothyroidism have consistently reported symptoms of severe cognitive impairments. In patients suffering hypothyroidism, thyroid hormone supplementation offers the prospect to alleviate the cognitive consequences of hypothyroidism; however, the therapeutic value of TH supplementation remains at present uncertain and the link between cellular modifications associated with hypothyroidism and neurodegeneration remains to be elucidated...
May 23, 2018: Molecular Neurobiology
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