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https://www.readbyqxmd.com/read/29314650/validation-of-reflex-testing-rules-and-establishment-of-a-new-workflow-for-body-fluid-cell-analysis-using-a-sysmex-xn-550-automatic-hematology-analyzer
#1
Y-U Cho, E You, S Jang, C-J Park
INTRODUCTION: We developed and validated reflex testing rules for the microscopic examination (ME) of body fluids (BFs) on the Sysmex XN-550 (Sysmex Corporation) instrument. METHODS: We assessed the detection limits, precision, linearity, and carryover. To develop the reflex testing rules (derivation arm), we tested 515 samples and then validated the rules using another 507 samples (validation arm). RESULTS: All analytical performances were acceptable, and the carryover was negligible...
January 4, 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29295661/neonatal-hyperbilirubinemia-secondary-to-combined-anti-e-and-anti-c-isoimmunisation-a-literature-review
#2
Deepak Sharma, Nazanin Farahbakhsh
A term male infant was admitted at 48 hours of postnatal life to the neonatal unit for jaundice. The investigation showed total serum bilirubin (TSB) of 17.1 mg/dl, haemoglobin of 11 g/dl, reticulocyte count of 9.5% and peripheral smear was suggestive of macrocytic, normochromic red blood cell (RBC) with target cells and multiple spherocytes with occasional nucleated RBC. The infant's blood group was B positive. Direct antiglobulin test was strongly positive by gel method (3+). Mother's blood group was B positive and indirect antiglobulin test was positive when tested postnatally...
January 2, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29228970/chemokine-and-cytokine-levels-in-the-lumbar-cerebrospinal-fluid-of-preterm-infants-with-post-hemorrhagic-hydrocephalus
#3
Gakwaya Habiyaremye, Diego M Morales, Clinton D Morgan, James P McAllister, Travis S CreveCoeur, Rowland H Han, Mohamed Gabir, Brandon Baksh, Deanna Mercer, David D Limbrick
BACKGROUND: Neuroinflammation has been implicated in the pathophysiology of post-hemorrhagic hydrocephalus (PHH) of prematurity, but no comprehensive analysis of signaling molecules has been performed using human cerebrospinal fluid (CSF). METHODS: Lumbar CSF levels of key cytokines (IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12, TNF-α, TGF-β1, IFN-γ) and chemokines (XCL-1, CCL-2, CCL-3, CCL-19, CXCL-10, CXCL-11, CXCL-12) were measured using conventional and multiplexed Enzyme-linked Immunosorbent Assays and compared between preterm infants with PHH and those with no known neurological injury...
December 12, 2017: Fluids and Barriers of the CNS
https://www.readbyqxmd.com/read/29219012/significance-of-assay-of-nucleated-rbcs-in-umbilical-cord-blood-in-neonates-with-meconium-stained-amniotic-fluid
#4
M Elsokkary, A Mamdouh, W Nossair, O Abd El Fattah, H Hemeda, S Sallam, M Taema, M Hussain, A Shafik, M Nawara, M Samy, M Abd El Aleem, R Abdelhadi, N Sakna, A Salama, D Salama, O El-Tohamy, N Elsaid
BACKGROUND: Approximately 8-15% of all infants are born with evidence of meconium-stained amniotic fluid (MSAF). MSAF is a potentially serious sign of fetal compromise and may indicate fetal hypoxia Objectives and aim of the work: The present study was designed to evaluate the relationship between meconium stained amniotic fluid and fetal nucleated red blood cell counts. As well, we aim to evaluate the relationship between the presence of meconium in amniotic fluid and Apgar scores in neonates...
December 8, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29213331/noninvasive-prenatal-diagnosis-of-fetal-aneuploidy-by-circulating-fetal-nucleated-red-blood-cells-and-extravillous-trophoblasts-using-silicon-based-nanostructured-microfluidics
#5
Chung-Er Huang, Gwo-Chin Ma, Hei-Jen Jou, Wen-Hsiang Lin, Dong-Jay Lee, Yi-Shing Lin, Norman A Ginsberg, Hsin-Fu Chen, Frank Mau-Chung Chang, Ming Chen
Background: Noninvasive prenatal testing (NIPT) based on cell-free DNA in maternal circulation has been accepted worldwide by the clinical community since 2011 but limitations, such as maternal malignancy and fetoplacental mosaicism, preclude its full replacement of invasive prenatal diagnosis. We present a novel silicon-based nanostructured microfluidics platform named as "Cell Reveal™" to demonstrate the feasibility of capturing circulating fetal nucleated red blood cells (fnRBC) and extravillous cytotrophoblasts (EVT) for cell-based noninvasive prenatal diagnosis (cbNIPD)...
2017: Molecular Cytogenetics
https://www.readbyqxmd.com/read/29207722/thalassaemia-trait-with-gaucher-disease-a-diagnostic-dilemma
#6
Jyoti Ramnath Kini, Saraswathy Sreeram, Anupama Hegde, Sowmini Kamath, Radha Ramachandra Pai
Gaucher Disease is an autosomal recessive disease caused by the accumulation of glucocerebrosidase due to deficiency in lysosomal glucocerebrosidase. Thalassaemia trait is asymptomatic and is usually an incidental diagnosis. Both thalassaemia and Gaucher disease can have similar haematologic manifestations and hence, their coexistence causes diagnostic dilemma. Our patient presented at one-and-a-half years with weakness, pallor, failure to thrive and massive hepatosplenomegaly. Clinical examination and history pointed to a lipid storage disease...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29205375/comparison-of-direct-in-house-cerebrospinal-fluid-cytology-with-commercial-pathology-results-in-dogs
#7
P L Newton, D R Fry, M P Best
OBJECTIVES: To investigate the correspondence between in-house direct cytological assessment of cerebrospinal fluid and results from a commercial veterinary pathology laboratory. METHODS: Prospective inclusion of samples from dogs that were presented for investigation of suspected neurological disease and had cerebrospinal fluid samples submitted to a commercial pathology laboratory for analysis. A board-certified veterinary pathologist assessed all cerebrospinal fluid samples, and a line smear was assessed in-house by two observers...
December 2017: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/29185049/a-radial-pillar-device-rapid-for-continuous-and-high-throughput-separation-of-multi-sized-particles
#8
Ninad Mehendale, Oshin Sharma, Claudy D'Costa, Debjani Paul
Pillar-based microfluidic sorting devices are preferred for isolation of rare cells due to their simple designs and passive operation. Dead-end pillar filters can efficiently capture large rare cells, such as, circulating tumor cells (CTCs), nucleated red blood cells (NRBCs), CD4 cells in HIV patients, etc., but they get clogged easily. Cross flow filters are preferred for smaller rare particles (e.g. separating bacteria from blood), but they need additional buffer inlets and a large device footprint for efficient operation...
November 28, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29163526/nucleated-teleost-erythrocytes-play-an-nk-lysin-and-autophagy-dependent-role-in-antiviral-immunity
#9
Patricia Pereiro, Alejandro Romero, Patricia Díaz-Rosales, Amparo Estepa, Antonio Figueras, Beatriz Novoa
With the exception of mammals, vertebrate erythrocytes are nucleated. Nevertheless, these cells are usually considered as mere carriers of hemoglobin. In this work, however, we describe for the first time an unrecognized role of teleost red blood cells (RBCs). We found that Nk-lysin (Nkl), an antimicrobial peptide produced by NK-cells and cytotoxic T-lymphocytes, was also expressed in flatfish turbot (Scophthalmus maximus) erythrocytes. Although the antiviral role of Nkl remains to be elucidated, we found a positive correlation between the transcription of nkl and the resistance to an infection with Rhabdovirus in a teleost fish...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29128605/erythrocytes-as-a-biological-model-for-screening-of-xenobiotics-toxicity
#10
REVIEW
Mayada Ragab Farag, Mahmoud Alagawany
Erythrocytes are the main cells in circulation. They are devoid of internal membrane structures and easy to be isolated and handled providing a good model for different assays. Red blood cells (RBCs) plasma membrane is a multi-component structure that keeps the cell morphology, elasticity, flexibility and deformability. Alteration of membrane structure upon exposure to xenobiotics could induce various cellular abnormalities and releasing of intracellular components. Therefore the morphological changes and extracellular release of haemoglobin [hemolysis] and increased content of extracellular adenosine triphosphate (ATP) [as signs of membrane stability] could be used to evaluate the cytotoxic effects of various molecules...
January 5, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29089557/high-throughput-assessment-of-mechanical-properties-of-stem-cell-derived-red-blood-cells-toward-cellular-downstream-processing
#11
Ewa Guzniczak, Maryam Mohammad Zadeh, Fiona Dempsey, Melanie Jimenez, Henry Bock, Graeme Whyte, Nicholas Willoughby, Helen Bridle
Stem cell products, including manufactured red blood cells, require efficient sorting and purification methods to remove components potentially harmful for clinical application. However, standard approaches for cellular downstream processing rely on the use of specific and expensive labels (e.g. FACS or MACS). Techniques relying on inherent mechanical and physical properties of cells offer high-throughput scalable alternatives but knowledge of the mechanical phenotype is required. Here, we characterized for the first time deformability and size changes in CD34+ cells, and expelled nuclei, during their differentiation process into red blood cells at days 11, 14, 18 and 21, using Real-Time Deformability Cytometry (RT-DC) and Atomic Force Microscopy (AFM)...
October 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29076090/analysis-of-erythropoiesis-using-imaging-flow-cytometry
#12
Theodosia Kalfa, Kathleen E McGrath
Erythroid maturation has been classically defined based on the remarkable changes visualized through microscopy. These involve the decrease in cell size, nuclear condensation and organelle loss, and include the final unique asymmetric division creating the short-lived nucleated pyrenocyte and the enucleate reticulocyte that matures into the red blood cell. Understanding the regulation of these processes has been challenging due to the difficulty in obtaining sufficient numbers of cells, particularly of rare intermediates, to study by microscopy...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29074889/unravelling-pathways-downstream-sox6-induction-in-k562-erythroid-cells-by-proteomic-analysis
#13
Gloria Barbarani, Antonella Ronchi, Margherita Ruoppolo, Lucia Santorelli, Robert Steinfelder, Sudharshan Elangovan, Cristina Fugazza, Marianna Caterino
The Sox6 transcription factor is crucial for terminal maturation of definitive red blood cells. Sox6-null mouse fetuses present misshapen and nucleated erythrocytes, due to impaired actin assembly and cytoskeleton stability. These defects are accompanied with a reduced survival of Sox6(-/-) red blood cells, resulting in a compensated anemia. Sox6-overexpression in K562 cells and in human primary ex vivo erythroid cultures enhances erythroid differentiation and leads to hemoglobinization, the hallmark of erythroid maturation...
October 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29070145/-effect-of-erk1-2-signaling-pathway-inhibitor-pd98059-on-the-expression-of-ras-braf-mek-erk1-2-in-marrow-nucleated-red-blood-cells-of-cms-patients
#14
Yuan-Fang Han, Lin-Hua Ji, Ting-Ting Feng, Fang Liu, Sen Cui, Juan Su
OBJECTIVE: To investigate the effect of ERK1 / 2 signaling pathway inhibitor PD98059 on Ras, Raf, MEK, ERK1, ERK2 expression in order to explore a new way for basic research and clinical treatment of the chronic mountain sickness(CMS). METHODS: Sixteen CMS patients were selected, the bone marrow was collected for isolation of monomuclear cells (MNC), the cells were sorted by using CD71 and CD235a antibody magnetic beads, then positive cells were diveded into 5 groups: blank control, DMSO and PD98059 5, 10 and 20 µmol/L, and were cultured in hypoxid condition for 72 hours...
October 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29055896/sifd-as-a-novel-cause-of-severe-fetal-hydrops-and-neonatal-anaemia-with-iron-loading-and-marked-extramedullary-haemopoiesis
#15
Chris Barton, Sabiha Kausar, Deborah Kerr, Stefania Bitetti, Rob Wynn
SIFD describes a heritable, syndromic condition characterised principally by sideroblastic anaemia (SA) with immunodeficiency, fevers and developmental delay, arising in mutations within the TRNT1 gene. Other clinical manifestations of SIFD include cardiomyopathy, seizures, sensorineural hearing loss, renal dysfunction, metabolic abnormalities, hepatosplenomegaly and retinitis pigmentosa.Presentation of SIFD is variable but typically in early childhood with SA or with fever. In this report, we extend the described SIFD phenotype...
October 21, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29044529/automated-identification-of-normoblast-cell-from-human-peripheral-blood-smear-images
#16
Dev Kumar DAS, Asok Kumar Maiti, Chandan Chakraborty
In this paper, we have presented a new computer-aided technique for automatic detection of nucleated red blood cells (NRBCs) or normoblast cell from peripheral blood smear image. The proposed methodology initiates with the localization of the nucleated cells by adopting multilevel thresholding approach in smear images. A novel colour space transformation technique has been introduced to differentiate nucleated blood cells [white blood cells (WBCs) and NRBC] from red blood cells (RBCs) by enhancing the contrast between them...
October 17, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28990291/performance-evaluation-of-the-automated-nucleated-red-blood-cell-count-of-five-commercial-hematological-analyzers
#17
G Da Rin, M Vidali, F Balboni, A Benegiamo, M Borin, M L Ciardelli, F Dima, A Di Fabio, A Fanelli, F Fiorini, S Francione, L Germagnoli, M Gioia, T Lari, M Lorubbio, A Marini, A Papa, M Seghezzi, L Solarino, S Pipitone, E Tilocca, S Buoro
INTRODUCTION: Recent automated hematology analyzers (HAs) can identify and report nucleated red blood cells (NRBC) count as a separate population out of white blood cells (WBC). The aim of this study was to investigate the analytical performances of NRBC enumeration on five top of the range HAs. METHODS: We evaluated the within-run and between-day precision, limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) of XE-2100 and XN-module (Sysmex), ADVIA 2120i (Siemens), BC-6800 (Mindray), and UniCel DxH 800 (Beckman Coulter)...
October 8, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28980399/comparison-of-performance-and-abnormal-cell-flagging-of-two-automated-hematology-analyzers-sysmex-xn-3000-and-beckman-coulter-dxh-800
#18
S Genc, E Dervisoglu, S Erdem, O Arslan, M Aktan, B Omer
INTRODUCTION: The purpose was to evaluate the analytical performances of Sysmex XN 3000 and UniCel DxH 800 comparing the obtained results with manual counting and between each other. Also flagging capabilities of abnormal cells were compared for both analyzers. METHODS: Two thousand one hundred and forty-two whole-blood samples were analyzed for evaluation. The samples flagged due to blast, atypical lymphocyte (AL), immature granulocyte (IG), or nucleated red blood cells (NRBC) were microscopically reviewed (n=102)...
October 4, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28969191/an-attempt-to-make-platelet-rich-fibrin-from-bone-marrow-aspirate
#19
Soh Nishimoto, Kazutoshi Fujita, Toshihiro Fujiwara, Yohei Sotsuka, Maki Tonooka, Hisako Ishise, Kenichiro Kawai, Masao Kakibuchi
INTRODUCTION: Platelet Rich Fibrin (PRF) was developed as a new generation of platelet concentration from peripheral blood. Method to make PRF is simple. It is easy to handle with its moderate firmness. Histologically, platelets and nucleated cells are packed along the yellow-red border. Bone marrow aspirate contains bone marrow cells that potentially work for tissue regeneration, and platelets which contain growth factors. The specific gravities of them are comparable. It implies that, if it is possible to make PRF from bone marrow aspirate, then high concentration of platelets and bone marrow cells can be obtained simultaneously by taking out yellow-red interface of it...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28890905/single-versus-multiple-site-harvesting-techniques-for-bone-marrow-concentrate-evaluation-of-aspirate-quality-and-pain
#20
Kristin Oliver, Tariq Awan, Matthew Bayes
BACKGROUND: Bone marrow concentrate (BMC) is growing in popularity as an alternative treatment option in orthopaedics. The regenerative capacity of BMC has been linked to the number of mesenchymal stem cells (MSCs) present in the graft at the time of its clinical application. MSC counts in bone marrow aspirate (BMA) are affected by harvest technique, but controversy exists over which aspiration method optimizes cellular yield while taking patient comfort and risk into consideration. PURPOSE: To compare a single- versus multiple-site bone marrow aspiration technique to determine which would generate a sufficient volume of high-quality BMA for concentration into a BMC graft...
August 2017: Orthopaedic Journal of Sports Medicine
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