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Nucleated red blood cells

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https://www.readbyqxmd.com/read/28079317/human-umbilical-cord-blood-cd34-positive-cells-as-predictors-of-the-incidence-and-short-term-outcome-of-neonatal-hypoxic-ischemic-encephalopathy-a-pilot-study
#1
Sahar M A Hassanein, Mohamed Hassan Nasr Eldin, Hanaa A Amer, Adel E Abdelhamid, Moustafa El Houssinie, Abir Ibrahim
BACKGROUND AND PURPOSE: Neonatal hypoxic-ischemic encephalopathy (HIE) is one of the leading causes of neurological handicap in developing countries. Human umbilical cord blood (hUCB) CD34-positive (CD34⁺) stem cells exhibit the potential for neural repair. We tested the hypothesis that hUCB CD34⁺ stem cells and other cell types [leukocytes and nucleated red blood cells (NRBCs)] that are up-regulated during the acute stage of perinatal asphyxia (PA) could play a role in the early prediction of the occurrence, severity, and mortality of HIE...
January 2017: Journal of Clinical Neurology
https://www.readbyqxmd.com/read/28057383/reference-values-for-nucleated-red-blood-cells-and-serum-lactate-in-very-and-extremely-low-birth-weight-infants-in-the-first-week-of-life
#2
Martin Poryo, Antonia Wissing, Aylin Aygün, Jürgen Geisel, Stefan Wagenpfeil, Michael Zemlin, Sascha Meyer
STUDY PURPOSE: To provide reference values for nucleated red blood cells and serum lactate concentrations in very and extremely low birth weight (VLBW/ELBW) infants in the first week of life. PATIENTS AND METHODS: Retrospective data analysis of serial, daily measurements of NRBC counts and serum lactate during the first 6days of life in VLBW and ELBW infants. RESULTS: In total, 250 infants<1500g were included in this study. Intrauterine growth retardation (IUGR) was seen in 87 (34...
January 2, 2017: Early Human Development
https://www.readbyqxmd.com/read/28050380/coincidental-finding-of-beta-thalassaemia-minor-in-a-patient-of-lacquer-thinner-poisoning-presenting-as-methaemoglobinemia
#3
Charu Agarwal, Anisha Mohanpuria, Gurdeep Buxi, Vijay Kumar
Lacquer thinner, commonly used for removing household paints, is known to contain various aromatic hydrocarbons and naphtha; if ingested, may cause methaemoglobinemia. We report a case of 13-year-old girl who presented with episodes of vomiting, abdominal pain and numbness of limbs. Peripheral blood smear showed few fragmented erythrocytes and 10-12 nucleated red blood cells /100 white blood cells. Reticulocyte count was 15% with presence of Heinz bodies. There was a history of accidental ingestion of lacquer paint thinner...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28042208/utility-of-peripheral-film-findings-and-its-correlation-with-automated-analyzer-an-audit-from-tertiary-care-hospital
#4
Sidra Asad, Imran Ahmed, Natasha Ali
BACKGROUND AND OBJECTIVE: With the advent of automated hematology analyzer, the use of traditional microscopy of blood film has become limited. The objective of our study was to determine the percentage of peripheral blood smear review in our institution in the era of automation and to identify reasons of manual review. MATERIALS AND METHODS: This was a prospective audit from January 1, 2015, to January 15, 2015. Consecutive complete blood count (CBC) samples and peripheral smear requests made up the sample size...
January 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28034522/comparison-of-two-methodologies-for-the-enrichment-of-mononuclear-cells-from-thawed-cord-blood-products-the-automated-sepax-system-versus-the-manual-ficoll-method
#5
Indreshpal Kaur, Jane M Zulovich, Marissa Gonzalez, Kara M McGee, Nirmali Ponweera, Daljit Thandi, Enrique F Alvarez, Kathy Annandale, Frank Flagge, Katayoun Rezvani, Elizabeth Shpall
BACKGROUND AIMS: Umbilical cord blood (CB) is being used as a source of hematopoietic stem cells (HSCs) and immune cells to treat many disorders. Because these cells are present in low numbers in CB, investigators have developed strategies to expand HSCs and other immune cells such as natural killer (NK) cells. The initial step in this process is to enrich mononuclear cells (MNCs) while depleting unwanted cells. The manual method of MNC enrichment is routinely used by many centers; however, it is an open system, time-consuming and operator dependent...
December 26, 2016: Cytotherapy
https://www.readbyqxmd.com/read/28018860/evidencing-the-role-of-erythrocytic-apoptosis-in-malarial-anemia
#6
REVIEW
Paulo R R Totino, Cláudio T Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz
In the last decade it has become clear that, similarly to nucleated cells, enucleated red blood cells (RBCs) are susceptible to programmed apoptotic cell death. Erythrocytic apoptosis seems to play a role in physiological clearance of aged RBCs, but it may also be implicated in anemia of different etiological sources including drug therapy and infectious diseases. In malaria, severe anemia is a common complication leading to death of children and pregnant women living in malaria-endemic regions of Africa. The pathogenesis of malarial anemia is multifactorial and involves both ineffective production of RBCs by the bone marrow and premature elimination of non-parasitized RBCs, phenomena potentially associated with apoptosis...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28018448/a-2-month-old-boy-with-hemolytic-anemia-and-reticulocytopenia-following-intravenous-immunoglobulin-therapy-for-kawasaki-disease-a-case-report-and-literature-review
#7
Na Yeon Kim, Joon Hwan Kim, Jin Suk Park, Soo Hyun Kim, Yeon Kyung Cho, Dong Hyun Cha, Ki Eun Kim, Myung Suh Kang, Kyung Ah Lim, Youn Ho Sheen
Herein, we report a rare case of hemolytic anemia with reticulocytopenia following intravenous immunoglobulin therapy in a young infant treated for Kawasaki disease. A 2-month-old boy presented with fever lasting 3 days, conjunctival injection, strawberry tongue, erythematous edema of the hands, and macular rash, symptoms and signs suggestive of incomplete Kawasaki disease. His fever resolved 8 days after treatment with aspirin and high dose infusion of intravenous immunoglobulin. The hemoglobin and hematocrit decreased from 9...
November 2016: Korean Journal of Pediatrics
https://www.readbyqxmd.com/read/27917462/iron-overload-across-the-spectrum-of-non-transfusion-dependent-thalassaemias-role-of-erythropoiesis-splenectomy-and-transfusions
#8
John B Porter, Maria Domenica Cappellini, Antonis Kattamis, Vip Viprakasit, Khaled M Musallam, Zewen Zhu, Ali T Taher
Non-transfusion-dependent thalassaemias (NTDT) encompass a spectrum of anaemias rarely requiring blood transfusions. Increased iron absorption, driven by hepcidin suppression secondary to erythron expansion, initially causes intrahepatic iron overload. We examined iron metabolism biomarkers in 166 NTDT patients with β thalassaemia intermedia (n = 95), haemoglobin (Hb) E/β thalassaemia (n = 49) and Hb H syndromes (n = 22). Liver iron concentration (LIC), serum ferritin (SF), transferrin saturation (TfSat) and non-transferrin-bound iron (NTBI) were elevated and correlated across diagnostic subgroups...
December 5, 2016: British Journal of Haematology
https://www.readbyqxmd.com/read/27896557/cytotoxic-and-apoptotic-activities-of-extract-of-amaranthus-spinosus-l-in-allium-cepa-and-human-erythrocytes
#9
V Prajitha, J E Thoppil
The present study examined the apoptosis inducing effects of Amaranthus spinosus L. aqueous extract in Allium cepa root meristematic cells and human erythrocytes. Cytogenetic assay revealed many apoptosis inducing cytogenetic aberrations viz., cytoplasmic breakage, cytoplasmic disintegration, cytoplasmic shrinkage, receding of cytoplasm, cytoplasmic vacuolation, enucleated cell, ghost cell, nuclear vacuolation, nuclear fragmentation and nuclear disintegration. A remarkable modification of red blood cell surface morphology was observed in the result of RBC assay...
November 28, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27882072/radix-astragali-stimulates-p38-mark-phosphorylation-in-pediatric-patients-with-%C3%AE-thalassemia
#10
Zhuoming Lu, Xinhua Qian, Chunhong Zhang, Zhiwen Chen, Guangliang Du
A previous study conducted by our group demonstrated that Radix Astragali compounded with Codonopsis pilosula and Plastrum testudinis was effective in treating pediatric β-thalassemia in a randomized, controlled clinical trial. However, the mechanism of action that underpins this treatment remains to be elucidated. Blood was collected from patients participating in this clinical trial and nucleated red blood cell-enriched mononuclear cells were isolated to facilitate the extraction of RNA and protein. RT-PCR was used to monitor the expression of globin genes and p38 MAPK, and total and phosphorylated p38 MAPK expression was assessed using Western blot analysis...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27872769/mantle-cell-hyperplasia-of-peripheral-lymph-nodes-as-initial-manifestation-of-sickle-cell-disease
#11
Ahmad Monabbati, Sadat Noori, Akbar Safaei, Mani Ramzi, Seyedsajjad Eghbali, Ali Adib
Sickle cell disease (SCD) is a well known hemoglobinopathy with usual manifestations including anemia, hyperbilirubinemia, and vasoocclusive complications. Despite presence of mild splenomegaly in early phase of the disease, lymphadenopathy is not an often finding of SCD. We introduce an undiagnosed case of SCD who presented in third decade of his life with multiple cervical lymphadenopathies and mild splenomegaly persistent for about five years. Histopathologic examination of the resected lymph nodes showed expansion of the mantle cell layers of secondary follicles as well as several monomorphic mantle cell nodules...
2016: Case Reports in Hematology
https://www.readbyqxmd.com/read/27850251/612-red-cell-distribution-width-is-associated-with-nucleated-red-blood-cells-in-surgical-icu-patients
#12
Tiffany Otero, Andrea Tsai, Daniel Yeh, Ruben Azocar, Donna Belcher, Sadeq Quraishi
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27843454/localisation-of-lactate-transporters-in-rat-and-rabbit-placentae
#13
Nigel P Moore, Catherine A Picut, Jeffrey H Charlap
The distribution of monocarboxylate transporter (MCT) isoforms 1 and 4, which mediate the plasmalemmal transport of l-lactic and pyruvic acids, has been identified in the placentae of rats and rabbits at different ages of gestation. Groups of three pregnant Sprague-Dawley rats and New Zealand White rabbits were sacrificed on gestation days (GD) 11, 14, 18, or 20 and on GD 13, 18, or 28, respectively. Placentae were removed and processed for immunohistochemical detection of MCT1 and MCT4. In the rat, staining for MCT1 was associated with lakes and blood vessels containing enucleated red blood cells (maternal vessels) while staining for MCT4 was associated with vessels containing nucleated red blood cells (embryofoetal vessels)...
2016: International Journal of Cell Biology
https://www.readbyqxmd.com/read/27809621/nucleated-red-blood-cells-count-as-a-prognostic-biomarker-in-predicting-the-complications-of-asphyxia-in-neonates
#14
Hassan Boskabadi, Maryam Zakerihamidi, Mohammad Hadi Sadeghian, Amir Avan, Majid Ghayour-Mobarhan, Gordon A Ferns
OBJECTIVE: Nucleated-red-blood-cells (NRBC) count in umbilical cord of newborns is been suggested as a sign of birth asphyxia. The present study was conducted to explore the value of NRBC count in prognosis of asphyxiated neonates. METHODS: sixty-three neonates with asphyxia were followed up for two years. Maternal and neonatal information was recorded follow by clinical and laboratory evaluation. NRBC-level was determined per 100 white-blood-cells (WBC). After discharge, follow-up of asphyxiated infants was performed using Denver II test at 6, 12, 18 and 24 months...
November 3, 2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27802412/cytographic-changes-on-bc-6800-haematological-analyzer-related-to-the-presence-of-candida-albicans-in-peripheral-blood-a-new-tool-to-suspect-candidemia
#15
Antonio La Gioia, Alessandra Devito, Fabiana Fiorini, Maria Bombara, Patrizia Isola, Barbara Spinale, Lucia Francioni, Domenico Salamone, Paola Marelli, Sabrina Buoro, Marcello Fiorini
BACKGROUND: We studied the quantitative and cytographic changes that the presence of Candida albicans (C. albicans) in peripheral blood (PB) samples causes on the Mindray BC-6800 Haematological Analyzer. METHODS: A simulated in vitro candidemia was obtained by adding a different amount of C. albicans to discarded remnants of PB samples. Quantitative data and cytographic features were evaluated immediately as well as after 120 and 240 min of the yeast addition...
October 31, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27795297/e2f-2-promotes-nuclear-condensation-and-enucleation-of-terminally-differentiated-erythroblasts
#16
Kelsey L Swartz, Scott N Wood, Tushar Murthy, Oscar Ramirez, Gangjian Qin, Manoj M Pillai, Sridhar Rao, Alex C Minella
E2F-2 is a retinoblastoma (Rb)-regulated transcription factor induced during terminal erythroid maturation. Cyclin E-mediated Rb hyperphosphorylation induces E2F transcriptional activator functions. We previously reported that deregulated cyclin E activity causes defective terminal maturation of nucleated erythroblasts in vivo Here, we found that these defects are normalized by E2F-2 deletion; however, anemia in mice with deregulated cyclin E is not improved by E2F-2-loss, which itself causes reduced peripheral red blood cell (RBC) counts without altering relative abundances of erythroblast subpopulations...
January 1, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27751864/evaluation-of-the-lh780-hematology-analyzer-for-detection-and-therapeutic-monitoring-of-malaria-cross-reactivity-with-nucleated-rbcs
#17
Jung Yoon, Seung Gyu Yun, Jeeyong Kim, Yoo Na Jung, Young Eun Koh, Jeong Hun Nam, Eun Taek Han, Chae Seung Lim
We evaluated the diagnostic usefulness of the LH780 Coulter blood cell counter for detection and therapeutic monitoring of malaria including cross-reactivity with nucleated RBC (NRBC) samples. A total of 405 patients (43 patients with Plasmodium vivax malaria and the control group of 120 healthy subjects, 111 patients with fever, and 131 patients with NRBCs) were analyzed with routine CBC using the LH780. We analyzed the CBC results according to three selected parameters: an abnormal peak in the WBC histogram before 35fL, the presence of red dots in the nonwhite cell zone of 2D WBC Diff Dataplot, and platelet-related flags suggesting platelet clumps or giant platelets...
December 2016: Acta Tropica
https://www.readbyqxmd.com/read/27690727/cord-blood-erythropoietin-and-cord-blood-nucleated-red-blood-cells-for-prediction-of-adverse-neonatal-outcome-associated-with-maternal-obesity-in-term-pregnancy-prospective-cohort-study
#18
Mahmoud H Ibrahim, Asmaa N Moustafa, Ahmed A Fadil Saedii, Ebtesam E Hassan
OBJECTIVE: To determine the adverse pregnancy outcomes associated with maternal prepregnancy overweight and obesity and we measure cord blood erythropoietin and NRBC count as indices of hypoxia and predictors of neonatal outcome. STUDY DESIGN: This prospective cohort study was done in Minia University Hospital, carried out from May 2015 to April 2016.270 full term neonates born to mothers of various body mass indices were included .Excluded were neonates with major factors known to be associated with a potential increase in fetal erythropoiesis...
October 3, 2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27687885/characterizing-the-hypomethylated-dna-methylation-profile-of-nucleated-red-blood-cells-from-cord-blood
#19
Olivia M de Goede, Pascal M Lavoie, Wendy P Robinson
AIM: To provide insight into fetal nucleated red blood cell (nRBC) development using genome-wide DNA methylation (DNAm) profiling. MATERIALS & METHODS: The DNAm profile (Illumina 450K array) of cord blood (n = 7) derived nRBCs was compared with B cells, CD4 and CD8 T cells, natural killer cells, granulocytes, monocytes and placenta (n = 5). RESULTS: nRBCs and placenta had similarly low array-wide DNAm compared with white blood cells, but their patterns of hypomethylation differed at biologically relevant subsets of the array...
September 30, 2016: Epigenomics
https://www.readbyqxmd.com/read/27668573/validation-of-a-dna-methylation-reference-panel-for-the-estimation-of-nucleated-cells-types-in-cord-blood
#20
Andres Cardenas, Catherine Allard, Myriam Doyon, E Andres Houseman, Kelly M Bakulski, Patrice Perron, Luigi Bouchard, Marie-France Hivert
Cord blood is widely used as surrogate tissue in epigenome-wide association studies of prenatal conditions. Cell type composition variation across samples can be an important confounder of epigenome-wide association studies in blood that constitute a mixture of cells. We evaluated a newly developed cord blood reference panel to impute cell type composition from DNA methylation levels, including nucleated red blood cells (nRBCs). We estimated cell type composition from 154 unique cord blood samples with available DNA methylation data as well as direct measurements of nucleated cell types...
September 26, 2016: Epigenetics: Official Journal of the DNA Methylation Society
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