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Nucleated red blood cells

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https://www.readbyqxmd.com/read/29748592/novel-erythrocyte-clumps-revealed-by-an-orphan-gene-newtic1-in-circulating-blood-and-regenerating-limbs-of-the-adult-newt
#1
Roman M Casco-Robles, Akihiko Watanabe, Ko Eto, Kazuhito Takeshima, Shuichi Obata, Tsutomu Kinoshita, Takashi Ariizumi, Kei Nakatani, Tomoaki Nakada, Panagiotis A Tsonis, Martin M Casco-Robles, Keisuke Sakurai, Kensuke Yahata, Fumiaki Maruo, Fubito Toyama, Chikafumi Chiba
The newt, a group of urodele amphibians, has outstanding ability to repeatedly regenerate various body parts, even in the terrestrial life-stage. In this animal, when the limb is amputated, a cell mass named the blastema appears on the stump and eventually gives rise to a new functional limb. Erythrocytes (red blood cells) in most non-mammalian vertebrates, including the newt, preserve their nucleus throughout their life-span, although physiological roles of such nucleated erythrocytes, other than oxygen delivery, are not known...
May 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29714997/derivatives-of-ribosome-inhibiting-antibiotic-chloramphenicol-inhibit-the-biosynthesis-of-bacterial-cell-wall
#2
Sivan Louzoun Zada, Keith D Green, Sanjib K Shrestha, Ido M Herzog, Sylvie Garneau-Tsodikova, Micha Fridman
Here, we describe the preparation and evaluation of α,β-unsaturated carbonyl derivatives of the bacterial translation inhibiting antibiotic chloramphenicol (CAM). Compared to the parent antibiotic, two compounds containing α,β-unsaturated ketones (1 and 4) displayed a broader spectrum of activity against a panel of Gram-positive pathogens with a minimum inhibitory concentration range of 2-32 μg/mL. Interestingly, unlike the parent CAM, these compounds do not inhibit bacterial translation. Microscopic evidence and metabolic labeling of a cell wall peptidoglycan suggested that compounds 1 and 4 caused extensive damage to the envelope of Staphylococcus aureus cells by inhibition of the early stage of cell wall peptidoglycan biosynthesis...
May 1, 2018: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29698529/nucleated-red-blood-cells-immune-cell-mediators-of-the-antiviral-response
#3
Ivan Nombela, Maria Del Mar Ortega-Villaizan
No abstract text is available yet for this article.
April 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29687517/hounsfield-units-are-a-useful-predictor-of-pleural-effusion-cytological-type-in-dogs-but-not-in-cats
#4
Sarah J Woods, Mathieu Spriet, Noa Safra, Derek D Cissell, Dori L Borjesson
All categories of pleural effusion subjectively display as soft tissue opacity on computed tomography (CT). Quantitative measurement using Hounsfield units (HU) has the potential to bring additional information regarding the nature of the fluid in a noninvasive way. The purposes of this retrospective cross-sectional analytical study were to compare Hounsfield units of different pleural effusion categories in dogs and cats, assess association between specific cytologic parameters and Hounsfield units, and evaluate the effect of dependent vs...
April 23, 2018: Veterinary Radiology & Ultrasound
https://www.readbyqxmd.com/read/29671811/shape-shifted-red-blood-cells-a-novel-red-blood-cell-stage
#5
Verónica Chico, Sara Puente-Marin, Iván Nombela, Sergio Ciordia, María Carmen Mena, Begoña Carracedo, Alberto Villena, Luis Mercado, Julio Coll, María Del Mar Ortega-Villaizan
Primitive nucleated erythroid cells in the bloodstream have long been suggested to be more similar to nucleated red cells of fish, amphibians, and birds than the red cells of fetal and adult mammals. Rainbow trout Ficoll-purified red blood cells (RBCs) cultured in vitro undergo morphological changes, especially when exposed to stress, and enter a new cell stage that we have coined shape-shifted RBCs (shRBCs). We have characterized these shRBCs using transmission electron microscopy (TEM) micrographs, Wright⁻Giemsa staining, cell marker immunostaining, and transcriptomic and proteomic evaluation...
April 19, 2018: Cells
https://www.readbyqxmd.com/read/29668734/prognostic-value-of-hematological-parameters-in-patients-with-acute-myocardial-infarction-intrahospital-outcomes
#6
José Gildo de Moura Monteiro Júnior, Dilênia de Oliveira Cipriano Torres, Maria Cleide Freire Clementino da Silva, Cyntia Maria de Holanda Martins, Izadora Karina da Silva, Monique Evelyn Mendonça do Nascimento, Ana Célia Oliveira Dos Santos, Ulisses Ramos Montarroyos, Dário Celestino Sobral Filho
BACKGROUND: The intensity of the inflammatory response and hemodynamic repercussion in acute myocardial infarction causing the presence in the peripheral circulation of nucleated red blood cells (NRBCs), increases in mean platelet volume (MPV) and neutrophil to lymphocyte ratio (NLR) are associated with a poorer prognosis. The aim of this study was to assess the role of these hematological biomarkers as predictors of all causes of mortality during the hospitalization of patients with acute myocardial infarction...
2018: PloS One
https://www.readbyqxmd.com/read/29642539/in-silico-functional-networks-identified-in-fish-nucleated-red-blood-cells-by-means-of-transcriptomic-and-proteomic-profiling
#7
Sara Puente-Marin, Iván Nombela, Sergio Ciordia, María Carmen Mena, Verónica Chico, Julio Coll, María Del Mar Ortega-Villaizan
Nucleated red blood cells (RBCs) of fish have, in the last decade, been implicated in several immune-related functions, such as antiviral response, phagocytosis or cytokine-mediated signaling. RNA-sequencing (RNA-seq) and label-free shotgun proteomic analyses were carried out for in silico functional pathway profiling of rainbow trout RBCs. For RNA-seq, a de novo assembly was conducted, in order to create a transcriptome database for RBCs. For proteome profiling, we developed a proteomic method that combined: (a) fractionation into cytosolic and membrane fractions, (b) hemoglobin removal of the cytosolic fraction, (c) protein digestion, and (d) a novel step with pH reversed-phase peptide fractionation and final Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometric (LC ESI-MS/MS) analysis of each fraction...
April 9, 2018: Genes
https://www.readbyqxmd.com/read/29642424/on-chip-cell-staining-and-counting-platform-for-the-rapid-detection-of-blood-cells-in-cerebrospinal-fluid
#8
Yujin Lee, Byeongyeon Kim, Sungyoung Choi
Counting blood cells in cerebrospinal fluid (CSF) is indispensable for diagnosing several pathological conditions in the central nervous system, such as meningitis, even though collecting CSF samples is invasive. Cell counting methods, such as hemocytometer chambers and flow cytometers, have been used for CSF cell counting, but they often lack the sensitivity to detect low blood cell numbers. They also depend on off-chip, manual sample preparation or require bulky, costly equipment, thereby limiting their clinical utility...
April 7, 2018: Sensors
https://www.readbyqxmd.com/read/29589209/nucleated-red-blood-cells-as-predictors-of-mortality-in-patients-with-acute-respiratory-distress-syndrome-ards-an-observational-study
#9
Mario Menk, Lena Giebelhäuser, Gerald Vorderwülbecke, Martina Gassner, Jan A Graw, Björn Weiss, Mathias Zimmermann, Klaus-D Wernecke, Steffen Weber-Carstens
BACKGROUND: Nucleated red blood cells (NRBCs) in critically ill patients are associated with increased mortality and poor outcome. The aim of the present study was to evaluate the predictive value of NRBCs in patients with acute respiratory distress syndrome (ARDS). METHODS: This observational study was conducted at an ARDS referral center and included patients from 2007 to 2014. Daily NRBC counts were assessed and the predictive validity of NRBCs on mortality was statistically evaluated...
March 27, 2018: Annals of Intensive Care
https://www.readbyqxmd.com/read/29536573/application-of-the-cometchip-platform-to-assess-dna-damage-in-field-collected-blood-samples-from-turtles
#10
Peter Sykora, Ylenia Chiari, Andrew Heaton, Nickolas Moreno, Scott Glaberman, Robert W Sobol
DNA damage has been linked to genomic instability and the progressive breakdown of cellular and organismal homeostasis, leading to the onset of disease and reduced longevity. Insults to DNA from endogenous sources include base deamination, base hydrolysis, base alkylation, and metabolism-induced oxidative damage that can lead to single-strand and double-strand DNA breaks. Alternatively, exposure to environmental pollutants, radiation or ultra-violet light, can also contribute to exogenously derived DNA damage...
May 2018: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/29507621/non-invasive-prenatal-diagnosis-of-chromosomal-aneuploidies-and-microdeletion-syndrome-using-fetal-nucleated-red-blood-cells-isolated-by-nanostructure-microchips
#11
Chun Feng, Zhaobo He, Bo Cai, Jianhong Peng, Jieping Song, Xuechen Yu, Yue Sun, Jing Yuan, Xingzhong Zhao, Yuanzhen Zhang
Detection of detached fetal nucleated red blood cells (fNRBCs) in the maternal peripheral blood may serve as a prospective testing method competing with the cell-free DNA, in non-invasive prenatal testing (NIPT). Methods: Herein, we introduce a facile and effective lab-on-a-chip method of fNRBCs detection using a capture-releasing material that is composed of biotin-doped polypyrrole nanoparticles. To enhance local topographic interactions between the nano-components and fNRBC, a specific antibody, CD147, coated on the nanostructured substrate led to the isolation of fNRBCs from maternal peripheral blood...
2018: Theranostics
https://www.readbyqxmd.com/read/29495220/-study-on-apoptosis-cytochrome-c-and-mitochondrial-membrane-potential-in-cd71-nucleated-erythrocytes-in-patients-with-chronic-mountain-sickness
#12
S Y Wang, S Cui, Z Q Li, L H Ji, J Ma, H H Liu, G Y Zhang, S H Suo, R L Ge
Objective: To investigate the changes of CD71(+) nucleated erythrocyte apoptosis, cytochrome C (Cyt-C) and mitochondrial membrane potential (MMP) in bone marrow of chronic mountain sickness (CMS). Methods: 14 patients with CMS and 15 patients with simple old fracture were divided into CMS group and control group, respectively.Bone marrow mononuclear cells (BMMNC) were separated, marked with CD71 monoclonal antibody and stained with Annexin V-FITC/PI.Then the apoptotic index of CD71(+) nucleated erythrocytes was determined by flow cytometry...
February 13, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29451060/cord-blood-buffy-coat-dna-methylation-is-comparable-to-whole-cord-blood-methylation
#13
John Dou, Rebecca J Schmidt, Kelly S Benke, Craig Newschaffer, Irva Hertz-Picciotto, Lisa A Croen, Ana-Maria Iosif, Janine M LaSalle, M Daniele Fallin, Kelly M Bakulski
Cord blood DNA methylation is associated with numerous health outcomes and environmental exposures. Whole cord blood DNA reflects all nucleated blood cell types, while centrifuging whole blood separates red blood cells, generating a white blood cell buffy coat. Both sample types are used in DNA methylation studies. Cell types have unique methylation patterns and processing can impact cell distributions, which may influence comparability. We evaluated differences in cell composition and DNA methylation between cord blood buffy coat and whole cord blood samples...
2018: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/29397262/consequences-of-dysregulated-complement-regulators-on-red-blood-cells
#14
REVIEW
Astrid J F Thielen, Sacha Zeerleder, Diana Wouters
The complement system represents the first line of defense that is involved in the clearance of pathogens, dying cells and immune complexes via opsonization, induction of an inflammatory response and the formation of a lytic pore. Red blood cells (RBCs) are very important for the delivery of oxygen to tissues and are continuously in contact with complement proteins in the blood plasma. To prevent complement activation on RBCs, various complement regulatory proteins can be found in plasma and on the cell membrane...
January 31, 2018: Blood Reviews
https://www.readbyqxmd.com/read/29397159/placental-pathology-in-neonatal-stroke-a-retrospective-case-control-study
#15
Miya E Bernson-Leung, Theonia K Boyd, Emily E Meserve, Amy R Danehy, Kush Kapur, Cameron C Trenor, Laura L Lehman, Michael J Rivkin
OBJECTIVE: To assess the association of placental abnormalities with neonatal stroke. STUDY DESIGN: This retrospective case-control study at 3 academic medical centers examined placental specimens for 46 children with neonatal arterial or venous ischemic stroke and 99 control children without stroke, using a standard protocol. Between-group comparisons used χ2 and Fisher exact t test. Correlations used Spearman correlation coefficient. RESULTS: Case placentas were more likely than controls to meet criteria for ≥1 of 5 major categories of pathologic abnormality (89% vs 62%; OR, 5...
April 2018: Journal of Pediatrics
https://www.readbyqxmd.com/read/29391153/an-historical-note-on-the-cell-theory
#16
REVIEW
Domenico Ribatti
The development of the microscope was a precondition for the discovery of cells. This instrument magnifies objects too small to be seen by the naked eye. In 1673, the Dutch botanist, Anton van Leeuwenhoek, made a more advanced microscope and reported seeing a myriad of microscopic "animalcules" in water. He also made further studies of red blood cells and sperm cells. Most studies that followed were done on the easily studied plant tissues. Plant cells, rigidly encased in their cell walls, were ideal to study in situ...
March 1, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29374555/performance-evaluation-of-the-new-measurement-channels-on-the-automated-sysmex-xn-9000-hematology-analyzer
#17
E Schapkaitz, S Raburabu
BACKGROUND: The automated Sysmex XN-9000 hematology system has been designed to meet the throughput and efficiency requirements of high volume laboratories with predominantly abnormal samples. New measurement channels have been introduced namely the white cell nucleated (WNR), white cell differential (WDF), white cell precursor (WPC) and fluorescent platelet (PLT-F) channels. METHODS: The performance of the new measurement channels was evaluated with regards to precision, accuracy, linearity, carryover, throughput and stability...
March 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29314650/validation-of-reflex-testing-rules-and-establishment-of-a-new-workflow-for-body-fluid-cell-analysis-using-a-sysmex-xn-550-automatic-hematology-analyzer
#18
Y-U Cho, E You, S Jang, C-J Park
INTRODUCTION: We developed and validated reflex testing rules for the microscopic examination (ME) of body fluids (BFs) on the Sysmex XN-550 (Sysmex Corporation) instrument. METHODS: We assessed the detection limits, precision, linearity, and carryover. To develop the reflex testing rules (derivation arm), we tested 515 samples and then validated the rules using another 507 samples (validation arm). RESULTS: All analytical performances were acceptable, and the carryover was negligible...
January 4, 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29295661/neonatal-hyperbilirubinemia-secondary-to-combined-anti-e-and-anti-c-isoimmunisation-a-literature-review
#19
Deepak Sharma, Nazanin Farahbakhsh
A term male infant was admitted at 48 h of postnatal life to the neonatal unit for jaundice. The investigation showed total serum bilirubin (TSB) of 17.1 mg/dl, haemoglobin of 11 g/dl, reticulocyte count of 9.5% and peripheral smear was suggestive of macrocytic, normochromic red blood cell (RBC) with target cells and multiple spherocytes with occasional nucleated RBC. The infant's blood group was B positive. Direct antiglobulin test was strongly positive by gel method (3+). Mother's blood group was B positive and indirect antiglobulin test was positive when tested postnatally...
January 8, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29228970/chemokine-and-cytokine-levels-in-the-lumbar-cerebrospinal-fluid-of-preterm-infants-with-post-hemorrhagic-hydrocephalus
#20
Gakwaya Habiyaremye, Diego M Morales, Clinton D Morgan, James P McAllister, Travis S CreveCoeur, Rowland H Han, Mohamed Gabir, Brandon Baksh, Deanna Mercer, David D Limbrick
BACKGROUND: Neuroinflammation has been implicated in the pathophysiology of post-hemorrhagic hydrocephalus (PHH) of prematurity, but no comprehensive analysis of signaling molecules has been performed using human cerebrospinal fluid (CSF). METHODS: Lumbar CSF levels of key cytokines (IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12, TNF-α, TGF-β1, IFN-γ) and chemokines (XCL-1, CCL-2, CCL-3, CCL-19, CXCL-10, CXCL-11, CXCL-12) were measured using conventional and multiplexed Enzyme-linked Immunosorbent Assays and compared between preterm infants with PHH and those with no known neurological injury...
December 12, 2017: Fluids and Barriers of the CNS
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