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Nucleated red blood cells

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https://www.readbyqxmd.com/read/28326960/placental-pathologic-features-in-fetomaternal-hemorrhage-detected-by-flow-cytometry
#1
Natasha E Lewis, Laura Marszalek, Linda M Ernst
Background Fetomaternal hemorrhage (FMH) is a poorly understood entity that can have significant clinical effects. Flow cytometry is a reliable and relatively new method for FMH diagnosis. The objective of this study was to correlate placental pathology with FMH detected by flow cytometry. Methods All patients with available placentas and FMH flow cytometric testing performed from 2009 to 2015 were retrospectively reviewed. Cases were defined as ≥0.10% fetal red blood cells (RBCs) in the maternal circulation while controls contained <0...
March 2017: Pediatric and Developmental Pathology
https://www.readbyqxmd.com/read/28259788/bisphenol-a-bisphenol-s-bisphenol-f-and-bisphenol-af-induce-different-oxidative-stress-and-damage-in-human-red-blood-cells-in-vitro-study
#2
Aneta Maćczak, Monika Cyrkler, Bożena Bukowska, Jaromir Michałowicz
Bisphenol A (BPA) and its analogs are widely used in the production of various everyday use products, which leads to a common exposure of humans to these substances. The effect of bisphenols on oxidative stress parameters has not been described in detail in non-nucleated cells, therefore, we have decided to evaluate the impact of BPA and its analogs, i.e. bisphenol S (BPS), bisphenol F (BPF) and bisphenol AF (BPAF) on reactive oxygen species (ROS) formation, lipid peroxidation, glutathione (GSH) level and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in human erythrocytes...
March 1, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28253358/epithelial-stem-cells-are-formed-by-small-particles-released-from-particle-producing-cells
#3
Wuyi Kong, Xiao Ping Zhu, Xiu Juan Han, Mu Nuo, Hong Wang
Recent spatiotemporal report demonstrated that epidermal stem cells have equal potential to divide or differentiate, with no asymmetric cell division observed. Therefore, how epithelial stem cells maintain lifelong stem-cell support still needs to be elucidated. In mouse blood and bone marrow, we found a group of large cells stained strongly for eosin and containing coiled-tubing-like structures. Many were tightly attached to each other to form large cellular clumps. After sectioning, these large cell-clumps were composed of not cells but numerous small particles, however with few small "naked" nuclei...
2017: PloS One
https://www.readbyqxmd.com/read/28177519/methionine-35-sulfoxide-reduces-toxicity-of-a%C3%AE-in-red-blood-cell
#4
Cristiana Carelli-Alinovi, Francesco Misiti
The oxidation of Methionine residue in position 35 of Aβ to sulfoxide (Aβ-sulfoxide) has the ability to deeply modify wild type Aβ 1-42 (Aβ) neurotoxic action. Our previous studies suggest that in nucleated cells lower toxicity of Aβ-sulfoxide might result not from structural alteration, but from elevation of Methionine sulfoxide reductase A (MsrA) activity and mRNA levels. On these basis, we hypothesised that red blood cell (RBC), a cell devoid almost completely of MsrA activity, share with nucleated cells an antioxidant system induced by methionine 35 sulfoxide, responsible for the lower toxicity of Aβ-sulfoxide in RBC...
February 8, 2017: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28176612/comparison-of-the-sysmex-xt-2000iv-with-microscopic-differential-counts-of-canine-bone-marrow
#5
Iris Pernecker, Natali B Bauer, Sigrid Johannes, Melanie Ginder, Johannes H Harleman, Andreas Moritz
Canine bone marrow is frequently assessed in the advanced preclinical research environment. Automated analysis provides time savings and objectivity over the gold standard of microscopic (cytologic) evaluation. We compared the analysis of 90 canine bone marrow samples by the Sysmex XT-2000iV hematology analyzer (Sysmex Corp., Kobe, Japan) with cytologic evaluation. Gates for cell populations were created in the system's WBC/BASO channel. Variables "total nucleated red blood cells" (total_NRBC), "poly- and orthochromatic nucleated red blood cells" (poly_orth_NRBC), "total neutrophils" (total_NEUT), "mature neutrophils" (mature_NEUT), and myeloid-to-erythroid (M:E) ratio were compared with cytologic evaluation...
January 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28121747/relationship-between-some-single-nucleotide-polymorphism-and-response-to-hydroxyurea-therapy-in-iranian-patients-with-%C3%AE-thalassemia-intermedia
#6
Mehran Karimi, Tahereh Zarei, Sezaneh Haghpanah, Mohamad Moghadam, Ahmad Ebrahimi, Narges Rezaei, Ghazaleh Heidari, Afsaneh Vazin, Maryam Khavari, Hamid R Miri
OBJECTIVE: To evaluate the possible relationship between hydroxyurea (HU) response and some single-nucleotide polymorphism (SNP) in patients affected by β-thalassemia intermedia. MATERIALS AND METHODS: In this cross-sectional study, 100 β-thalassemia intermedia patients who were taking HU with a dose of 8 to 15 mg/kg body weight per day for a period of at least 6 months were randomly selected between February 2013 and October 2014 in southern Iran. HU response was defined based on decrease or cessation of the blood transfusion need and evaluation of Hb level...
January 24, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28119472/pseudomonas-aeruginosa-pore-forming-exolysin-and-type-iv-pili-cooperate-to-induce-host-cell-lysis
#7
Pauline Basso, Michel Ragno, Sylvie Elsen, Emeline Reboud, Guillaume Golovkine, Stephanie Bouillot, Philippe Huber, Stephen Lory, Eric Faudry, Ina Attrée
Clinical strains of Pseudomonas aeruginosa lacking the type III secretion system genes employ a toxin, exolysin (ExlA), for host cell membrane disruption. Here, we demonstrated that ExlA export requires a predicted outer membrane protein, ExlB, showing that ExlA and ExlB define a new active two-partner secretion (TPS) system of P. aeruginosa In addition to the TPS signals, ExlA harbors several distinct domains, which include one hemagglutinin domain, five arginine-glycine-aspartic acid (RGD) motifs, and a C-terminal region lacking any identifiable sequence motifs...
January 24, 2017: MBio
https://www.readbyqxmd.com/read/28095473/the-asymmetric-cell-division-regulators-par3-scribble-and-pins-gpsm2-are-not-essential-for-erythroid-development-or-enucleation
#8
Christina B Wölwer, Nathan Gödde, Luke B Pase, Imogen A Elsum, Krystle Y B Lim, Faruk Sacirbegovic, Carl R Walkley, Sarah Ellis, Shigeo Ohno, Fumio Matsuzaki, Sarah M Russell, Patrick O Humbert
Erythroid enucleation is the process by which the future red blood cell disposes of its nucleus prior to entering the blood stream. This key event during red blood cell development has been likened to an asymmetric cell division (ACD), by which the enucleating erythroblast divides into two very different daughter cells of alternate molecular composition, a nucleated cell that will be removed by associated macrophages, and the reticulocyte that will mature to the definitive erythrocyte. Here we investigated gene expression of members of the Par, Scribble and Pins/Gpsm2 asymmetric cell division complexes in erythroid cells, and functionally tested their role in erythroid enucleation in vivo and ex vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28091810/severe-lead-toxicosis-in-a-lionhead-rabbit
#9
Kristen M Walter, Karyn Bischoff, Ricardo de Matos
INTRODUCTION: Lead toxicosis occurs in veterinary patients, with few reports involving rabbits, and no previous reports using oral calcium disodium EDTA. CASE REPORT: A 7-year-old male castrated Lionhead rabbit presented to the Cornell University Hospital for Animals (CUHA) for evaluation after a 2-day history of lethargy and a 2-week history of hyporexia. The patient had been observed pulling paint from the walls of the home, a house built circa 1900, in the months prior to presentation...
March 2017: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
https://www.readbyqxmd.com/read/28079317/human-umbilical-cord-blood-cd34-positive-cells-as-predictors-of-the-incidence-and-short-term-outcome-of-neonatal-hypoxic-ischemic-encephalopathy-a-pilot-study
#10
Sahar M A Hassanein, Mohamed Hassan Nasr Eldin, Hanaa A Amer, Adel E Abdelhamid, Moustafa El Houssinie, Abir Ibrahim
BACKGROUND AND PURPOSE: Neonatal hypoxic-ischemic encephalopathy (HIE) is one of the leading causes of neurological handicap in developing countries. Human umbilical cord blood (hUCB) CD34-positive (CD34⁺) stem cells exhibit the potential for neural repair. We tested the hypothesis that hUCB CD34⁺ stem cells and other cell types [leukocytes and nucleated red blood cells (NRBCs)] that are up-regulated during the acute stage of perinatal asphyxia (PA) could play a role in the early prediction of the occurrence, severity, and mortality of HIE...
January 2017: Journal of Clinical Neurology
https://www.readbyqxmd.com/read/28057383/reference-values-for-nucleated-red-blood-cells-and-serum-lactate-in-very-and-extremely-low-birth-weight-infants-in-the-first-week-of-life
#11
Martin Poryo, Antonia Wissing, Aylin Aygün, Jürgen Geisel, Stefan Wagenpfeil, Michael Zemlin, Sascha Meyer
STUDY PURPOSE: To provide reference values for nucleated red blood cells and serum lactate concentrations in very and extremely low birth weight (VLBW/ELBW) infants in the first week of life. PATIENTS AND METHODS: Retrospective data analysis of serial, daily measurements of NRBC counts and serum lactate during the first 6days of life in VLBW and ELBW infants. RESULTS: In total, 250 infants<1500g were included in this study. Intrauterine growth retardation (IUGR) was seen in 87 (34...
January 2, 2017: Early Human Development
https://www.readbyqxmd.com/read/28050380/coincidental-finding-of-beta-thalassaemia-minor-in-a-patient-of-lacquer-thinner-poisoning-presenting-as-methaemoglobinemia
#12
Charu Agarwal, Anisha Mohanpuria, Gurdeep Buxi, Vijay Kumar
Lacquer thinner, commonly used for removing household paints, is known to contain various aromatic hydrocarbons and naphtha; if ingested, may cause methaemoglobinemia. We report a case of 13-year-old girl who presented with episodes of vomiting, abdominal pain and numbness of limbs. Peripheral blood smear showed few fragmented erythrocytes and 10-12 nucleated red blood cells /100 white blood cells. Reticulocyte count was 15% with presence of Heinz bodies. There was a history of accidental ingestion of lacquer paint thinner...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28042208/utility-of-peripheral-film-findings-and-its-correlation-with-automated-analyzer-an-audit-from-tertiary-care-hospital
#13
Sidra Asad, Imran Ahmed, Natasha Ali
BACKGROUND AND OBJECTIVE: With the advent of automated hematology analyzer, the use of traditional microscopy of blood film has become limited. The objective of our study was to determine the percentage of peripheral blood smear review in our institution in the era of automation and to identify reasons of manual review. MATERIALS AND METHODS: This was a prospective audit from January 1, 2015, to January 15, 2015. Consecutive complete blood count (CBC) samples and peripheral smear requests made up the sample size...
January 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28034522/comparison-of-two-methodologies-for-the-enrichment-of-mononuclear-cells-from-thawed-cord-blood-products-the-automated-sepax-system-versus-the-manual-ficoll-method
#14
Indreshpal Kaur, Jane M Zulovich, Marissa Gonzalez, Kara M McGee, Nirmali Ponweera, Daljit Thandi, Enrique F Alvarez, Kathy Annandale, Frank Flagge, Katayoun Rezvani, Elizabeth Shpall
BACKGROUND AIMS: Umbilical cord blood (CB) is being used as a source of hematopoietic stem cells (HSCs) and immune cells to treat many disorders. Because these cells are present in low numbers in CB, investigators have developed strategies to expand HSCs and other immune cells such as natural killer (NK) cells. The initial step in this process is to enrich mononuclear cells (MNCs) while depleting unwanted cells. The manual method of MNC enrichment is routinely used by many centers; however, it is an open system, time-consuming and operator dependent...
December 26, 2016: Cytotherapy
https://www.readbyqxmd.com/read/28018860/evidencing-the-role-of-erythrocytic-apoptosis-in-malarial-anemia
#15
REVIEW
Paulo R R Totino, Cláudio T Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz
In the last decade it has become clear that, similarly to nucleated cells, enucleated red blood cells (RBCs) are susceptible to programmed apoptotic cell death. Erythrocytic apoptosis seems to play a role in physiological clearance of aged RBCs, but it may also be implicated in anemia of different etiological sources including drug therapy and infectious diseases. In malaria, severe anemia is a common complication leading to death of children and pregnant women living in malaria-endemic regions of Africa. The pathogenesis of malarial anemia is multifactorial and involves both ineffective production of RBCs by the bone marrow and premature elimination of non-parasitized RBCs, phenomena potentially associated with apoptosis...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28018448/a-2-month-old-boy-with-hemolytic-anemia-and-reticulocytopenia-following-intravenous-immunoglobulin-therapy-for-kawasaki-disease-a-case-report-and-literature-review
#16
Na Yeon Kim, Joon Hwan Kim, Jin Suk Park, Soo Hyun Kim, Yeon Kyung Cho, Dong Hyun Cha, Ki Eun Kim, Myung Suh Kang, Kyung Ah Lim, Youn Ho Sheen
Herein, we report a rare case of hemolytic anemia with reticulocytopenia following intravenous immunoglobulin therapy in a young infant treated for Kawasaki disease. A 2-month-old boy presented with fever lasting 3 days, conjunctival injection, strawberry tongue, erythematous edema of the hands, and macular rash, symptoms and signs suggestive of incomplete Kawasaki disease. His fever resolved 8 days after treatment with aspirin and high dose infusion of intravenous immunoglobulin. The hemoglobin and hematocrit decreased from 9...
November 2016: Korean Journal of Pediatrics
https://www.readbyqxmd.com/read/27917462/iron-overload-across-the-spectrum-of-non-transfusion-dependent-thalassaemias-role-of-erythropoiesis-splenectomy-and-transfusions
#17
John B Porter, Maria Domenica Cappellini, Antonis Kattamis, Vip Viprakasit, Khaled M Musallam, Zewen Zhu, Ali T Taher
Non-transfusion-dependent thalassaemias (NTDT) encompass a spectrum of anaemias rarely requiring blood transfusions. Increased iron absorption, driven by hepcidin suppression secondary to erythron expansion, initially causes intrahepatic iron overload. We examined iron metabolism biomarkers in 166 NTDT patients with β thalassaemia intermedia (n = 95), haemoglobin (Hb) E/β thalassaemia (n = 49) and Hb H syndromes (n = 22). Liver iron concentration (LIC), serum ferritin (SF), transferrin saturation (TfSat) and non-transferrin-bound iron (NTBI) were elevated and correlated across diagnostic subgroups...
January 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/27896557/cytotoxic-and-apoptotic-activities-of-extract-of-amaranthus-spinosus-l-in-allium-cepa-and-human-erythrocytes
#18
V Prajitha, J E Thoppil
The present study examined the apoptosis inducing effects of Amaranthus spinosus L. aqueous extract in Allium cepa root meristematic cells and human erythrocytes. Cytogenetic assay revealed many apoptosis inducing cytogenetic aberrations viz., cytoplasmic breakage, cytoplasmic disintegration, cytoplasmic shrinkage, receding of cytoplasm, cytoplasmic vacuolation, enucleated cell, ghost cell, nuclear vacuolation, nuclear fragmentation and nuclear disintegration. A remarkable modification of red blood cell surface morphology was observed in the result of RBC assay...
February 2017: Cytotechnology
https://www.readbyqxmd.com/read/27882072/radix-astragali-stimulates-p38-mark-phosphorylation-in-pediatric-patients-with-%C3%AE-thalassemia
#19
Zhuoming Lu, Xinhua Qian, Chunhong Zhang, Zhiwen Chen, Guangliang Du
A previous study conducted by our group demonstrated that Radix Astragali compounded with Codonopsis pilosula and Plastrum testudinis was effective in treating pediatric β-thalassemia in a randomized, controlled clinical trial. However, the mechanism of action that underpins this treatment remains to be elucidated. Blood was collected from patients participating in this clinical trial and nucleated red blood cell-enriched mononuclear cells were isolated to facilitate the extraction of RNA and protein. RT-PCR was used to monitor the expression of globin genes and p38 MAPK, and total and phosphorylated p38 MAPK expression was assessed using Western blot analysis...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27872769/mantle-cell-hyperplasia-of-peripheral-lymph-nodes-as-initial-manifestation-of-sickle-cell-disease
#20
Ahmad Monabbati, Sadat Noori, Akbar Safaei, Mani Ramzi, Seyedsajjad Eghbali, Ali Adib
Sickle cell disease (SCD) is a well known hemoglobinopathy with usual manifestations including anemia, hyperbilirubinemia, and vasoocclusive complications. Despite presence of mild splenomegaly in early phase of the disease, lymphadenopathy is not an often finding of SCD. We introduce an undiagnosed case of SCD who presented in third decade of his life with multiple cervical lymphadenopathies and mild splenomegaly persistent for about five years. Histopathologic examination of the resected lymph nodes showed expansion of the mantle cell layers of secondary follicles as well as several monomorphic mantle cell nodules...
2016: Case Reports in Hematology
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