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H Sasaki, A Takamura, K Kawahata, T Takashima, K Imai, T Morio, H Kohsaka
OBJECTIVE: Dermatomyositis (DM) is an idiopathic inflammatory myopathy which often involves the lungs. DM is likely to be associated with aberrant T- and B-cell activation in the pathogenesis because of the proven effectiveness of T- and B-cell-targeted treatments. Assuming that the aberrant activation is reflected by biases in the lymphocyte subset repertoires, we aimed to elucidate these biases, especially in relation to clinical features of DM. METHOD: Based on the immunophenotyping standardized by the Human Immunology Project Consortium, untreated 13 DM patients, including seven patients with interstitial lung disease (ILD), and 18 age-matched healthy donors (HDs) were examined for proportions of peripheral blood lymphocyte subsets...
December 5, 2018: Scandinavian Journal of Rheumatology
Judith M Martin, Raghunandan Avula, Mary Patricia Nowalk, Chyongchiou Jeng Lin, William T Horne, Uma R Chandran, Jennifer P Nagg, Richard K Zimmerman, Kelly S Cole, John F Alcorn
Background: The reasons for differences in vaccine effectiveness between live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV) are not clear. Methods: Blood samples were obtained before vaccination and at days 7 and 21 postvaccination with 2015-2016 quadrivalent IIV or LAIV. Serologic response to the vaccine was measured by hemagglutination inhibition assay. Targeted RNA sequencing and serum cytokine analysis were performed. Paired analyses were used to determine gene expression and were compared between IIV and LAIV recipients...
November 2018: Open Forum Infectious Diseases
Liisi Raam, Epp Kaleviste, Marina Šunina, Helen Vaher, Mario Saare, Ele Prans, Maire Pihlap, Kristi Abram, Maire Karelson, Pärt Peterson, Ana Rebane, Kai Kisand, Külli Kingo
Vitiligo is a chronic multifactorial depigmentation disorder characterized by the destruction and functional loss of melanocytes. Although a direct cytotoxic T cell attack is thought to be responsible for melanocyte damage, the events leading to the loss of self-tolerance toward melanocytic antigens are not understood. This research aimed to identify novel cellular and molecular factors that participate in vitiligo pathogenesis through the application of gene expression and immunofluorescence analysis of skin biopsy samples along with immunophenotyping of circulating cells...
2018: Frontiers in Immunology
Zhen-Tang Lao, Ling Wen Ding, Omer An, Norimichi Hattori, Qiao-Yang Sun, Kar-Tong Tan, Anand Mayakonda, Wong Gee Chuan, Vikas Madan, De-Chen Lin, Henry Yang, H Phillip Koeffler
Acute leukemia of ambiguous lineage (ALAL) is a rare group of blood cancers that cannot be clearly classified into either myeloid or lymphoid lineage through traditional immunophenotyping (2016 World Health Organization classification). In this study, we performed exome and transcriptome sequencing of 15 diagnosis/relapse samples to identify mutations of this disease. Remarkably, genes involved in DNA repair pathway were frequently mutated and occurred in 80% of the samples. In addition, well known mutations of hematopoietic neoplasms were found in these samples, such as DNMT3A, RUNX1, NOTCH1 and NRAS...
December 4, 2018: Haematologica
Ga-Won Lee, Su-Bin Song, Min-Hee Kang, Hee-Myung Park
BACKGROUND: There is no specific therapy for cutaneous epitheliotropic T-cell lymphoma (CETL). The administration of retinoids in conjunction with interferon-α (IFN-α) in CETL has not been reported in dogs. CASE PRESENTATION: Two dogs (Shih tzu and Miniature pinscher) presented with multiple nodular skin lesions. Histopathological examination revealed diffuse infiltrations of lymphocytes in the epidermis and dermis, with a CD3-positive immunophenotypic profile...
December 4, 2018: BMC Veterinary Research
O Servitje, F Climent, L Colomo, N Ruiz, A García-Herrera, F Gallardo, S Mercadal, H Pomares, C Muniesa, C Martin-Callizo, J Marcoval, R Rovira, T Estrach, R M Pujol
BACKGROUND: Primary cutaneous follicular center-cell lymphoma (PCFCL) is one of the most common types of cutaneous B-cell lymphoma. Differences in immunohistochemical expression of BCL2 and CD10 antigens along with the presence of t(14:18) translocation in neoplastic cells have been postulated as relevant clues in differentiating PCFCL from cutaneous lesions secondary to a systemic follicular lymphoma (SCFL). The aim of this study is to evaluate the significance and usefulness of these parameters in a large series of patients...
December 4, 2018: Journal of Cutaneous Pathology
Irma Soldevilla-Gallardo, Sevastian S Medina-Ornelas, Cynthia Villarreal-Garza, Enrique Bargalló-Rocha, Claudia Hs Caro-Sánchez, Rodrigo Hernández-Ramírez, Enrique Estrada-Lobato
Our study examines the association between two Positron Emission Mammography (PEM) semi-quantitative parameters: PUVmax (maximum uptake value) and LTB (lesion to background) baseline and the end of Neoadjuvant chemotherapy (NAC) with pathologic response in each of the following breast cancer subtype: Triple negative breast cancer (TPN), HER2-positive, and ER-positive/HER2-negative cancers. One-hundred and eight patients, 71 with invasive ductal carcinoma and 37 with infiltrating lobular carcinoma were evaluate with 18 F-FDG-PEM scans before and after of NAC...
2018: American Journal of Nuclear Medicine and Molecular Imaging
Jiahao Chen, Yun-Ruei Kao, Daqian Sun, Tihomira I Todorova, David Reynolds, Swathi-Rao Narayanagari, Cristina Montagna, Britta Will, Amit Verma, And Ulrich Steidl
Myelodysplastic syndromes (MDS) frequently progress to acute myeloid leukemia (AML); however, the cells leading to malignant transformation have not been directly elucidated. As progression of MDS to AML in humans provides a biological system to determine the cellular origins and mechanisms of neoplastic transformation, we studied highly fractionated stem cell populations in longitudinal samples of patients with MDS who progressed to AML. Targeted deep sequencing combined with single-cell sequencing of sorted cell populations revealed that stem cells at the MDS stage, including immunophenotypically and functionally defined pre-MDS stem cells (pre-MDS-SC), had a significantly higher subclonal complexity compared to blast cells and contained a large number of aging-related variants...
December 3, 2018: Nature Medicine
Masayuki Hirano
The rapid advancement of next generation sequencing technology has resulted in accumulation of many datasets in cancer clinical and research laboratories, many of which do not have bioinformaticians. Xu and colleagues developed a user-friendly web-based tool to define the tumor immunophenotype among patients with cancer. By uploading user-defined datasets on the web, it can systematically track, analyze, and visualize the status of anticancer immune activity and the proportion of tumor-infiltrating immune cells...
December 1, 2018: Cancer Research
Mayra Ramos de Jesus Pereira, Valéria Rodrigues Pinhatti, Maiele Dornelles da Silveira, Cristina Araujo Matzenbacher, Thales Renato Ochotorena de Freitas, Juliana da Silva, Melissa Camassola, Nance Beyer Nardi
Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil...
November 29, 2018: Genetics and Molecular Biology
Kelly M Cox, Scott P Commins, Brian J Capaldo, Lisa J Workman, Thomas A E Platts-Mills, El-Ad D Amir, Josephine A Lannigan, Alexander J Schuyler, Loren D Erickson
BACKGROUND: B cells play a critical role in the development and maintenance of food allergy by producing allergen-specific IgE. Despite the importance of B cells in IgE-mediated food allergy, the identity of sIgE-producing human B cells and how IgE is regulated are poorly understood. OBJECTIVE: To identify the immunophenotypes of circulating B cells associated with the production of galactose-alpha-1,3-galactose specific IgE production in patients with red meat allergy...
December 2, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
M Sulce, L Marconato, M Martano, S Iussich, A Dentini, M Melega, B Miniscalco, F Riondato
Mast cell tumors (MCT) are among the most frequent tumors in dogs, but studies regarding canine mast cell immunophenotype are lacking. The aim of this study was to assess the feasibility of flow cytometric analysis of MCTs, to describe canine MCTs immunophenotype(s), and to evaluate the ability of flow cytometry to detect mast cells in lymph node aspirates. Thirty-four primary canine MCTs and 12 draining lymph nodes were evaluated regarding the expression of CD117, IgE, CD11b, CD18, CD44, CD34, CD25 and CD45...
December 2018: Veterinary Journal
ChouMin Chong, ChenFei Low
The emerging technology of aptamers that is also known as synthetic antibodies is rivalling antibodies research in the recent years. The unique yet important features of aptamers are advancing antibodies in diverse applications, which include disease diagnosis, prophylactic and therapeutic. The versatility of aptamer has further extended its application to function as gene expression modulator, known as synthetic riboswitches. This report reviewed and discussed the applications of aptamers technology in the biosecurity of aquaculture, the promising developments in biosensor detection for disease diagnosis as well as prophylactic and therapeutic measurements...
November 28, 2018: Fish & Shellfish Immunology
Yi-Xuan He, Si-Dan Li, Run-Hui Wu, Mao-Quan Qin
Transplantation-associated thrombotic microangiopathy (TA-TMA) is one of the fatal complications of hematopoietic stem cell transplantation(HSCT). The pathogenesis of TA-TMA has not been fully elucidated. The latest researches show that the abnormal activation of the complement system may lead to widespread endothelial injury which may play an important role in the pathogenesis of this disease. Incontrotable hypertension, proteinuria, increase of soluble C5b-9 concentration and early pericardial effusion are the risk factors of TA-TMA ...
December 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Fang Bao, Ka Hu, Wei Wan, Lei Tian, Hong-Mei Jing
OBJECTIVE: To investigate the efficacy of CD19-targeted chimeric antigen receptor T-cell (CD19-CAR T) in the treatment of patients with refractory relapsed B cell acute lymphocyte leukemia(B-ALL). METHODS: The efficacy and safety of CD19-CAR T cells in treatment of patients with refractory relapsed B-ALL from January 2015 to July 2017 in the Department of Hematology of Peking University Third Hospital were analyzed retrospectively. RESULTS: A total of 10 patients were included in this analysis, all of which were consistent with the diagnosis of refractory relapsed B-ALL, and the immunophenotype of leukemia cells was CD19 positive...
December 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Fan Wu, Ying Pan, Huiping Wang, Qianshan Tao, Furun An, Jiakui Zhang, Zhimin Zhai
Hepatosplenic T-cell lymphoma (HSTCL) is a very rare non-Hodgkin lymphoma with an aggressive clinical course and poor prognosis. Patients of this disease usually presented with hepatosplenomegaly, which can be misdiagnosed or delayed. Bone marrow (BM) and peripheral blood (PB) are frequently involved, however, central nervous system (CNS) involvement is less common. Here, we are reporting an unusual case of hepatosplenic γδ T-cell lymphoma in a 64-year-old man with CNS involvement. Flow cytometry immunophenotyping was proved of great diagnostic contribution...
November 30, 2018: Cytometry. Part B, Clinical Cytometry
Işik Odaman Al, Cengiz Bayram, Başak Koç, Gizem Ersoy, Ezgi Pasli Uysalol, Hilal Aki, Gül Nihal Özdemir
BACKGROUND: Burkitt leukemia (BL) with the precursor B-cell immunophenotype is a rarely reported condition. The prognosis of such patients is similar to that of classic BL. However, the combination of chromosomal translocations associated with bcl-2 and c-myc rearrangement has a poor prognosis. OBSERVATIONS: An 11-year-old child presented with fever and weakness. Bone marrow aspiration showed morphologically L1 type blasts and flow cytometry analysis was compatible with precursor B-cell immunophenotype...
November 28, 2018: Journal of Pediatric Hematology/oncology
Aleena A Saidova, Daria M Potashnikova, Anna V Tvorogova, Ivan V Maly, Wilma A Hofmann, Ivan A Vorobjev
Background: Prostate cancer (PC) diagnostics and treatment often present a challenging task due to cancer subtype heterogeneity and differential disease progression in patient subgroups. Hence, the critical issue is finding a reliable and sensitive diagnostic and prognostic PC marker, especially for cases of biopsies with low percentages of cancer cells. Isoform A of myosin 1C was shown to be expressed in PC cells and responsible for their invasive properties, however, its feasibility for diagnostic purposes remains to be elucidated...
2018: PeerJ
Neha Garg, Mrinalini Kotru, Dilip Kumar, Rajesh Pathak, Meera Sikka
INTRODUCTION: Aberrant expression of immunophenotypic markers is commonly found in patients of acute leukemia. T-ALL also shows aberrant markers such as CD13, CD33, CD117, CD10, and CD79a. Morphologically, T-ALL has been categorized into L1, L2, and L3 subtypes. Till now, no study has been done to correlate these markers with morphological features of T-ALL. This study aimed to correlate the expression of aberrant immunophenotypic markers with morphology in T-ALL. MATERIALS AND METHODS: All the cases of T-ALL diagnosed by flow cytometry over a period of 2½ year were taken out from the records of Hematology Section of Department of Pathology of University College of Medical Science and Guru Teg Bahadur Hospital and Max Hospital, Saket...
October 2018: Journal of Laboratory Physicians
Dina El Demellawy, James Yj Lee, Laura McDonell, David A Dyment, A S Knisely, Jean McGowan-Jordan, Bo Ngan, Milton Finegold, Raj P Kapur, Ahmed Nasr
Hepatic mesenchymal hamartoma is a rare benign neoplasm principally encountered in young children. Its origin is unknown. We report an unusual hepatic mesenchymal hamartoma in a 7-month-old girl, including histopathologic findings, immunophenotype, and karyotype. Chromosomal microarray analysis of tumoral tissue and circulating lymphocytes found 4 copies of a segment at 1q44 and fluorescence in situ hybridization indicated tandem triplication, ascribed to expansion of a paternal tandem duplication. This genetic abnormality may have played a role in pathogenesis...
November 29, 2018: Pediatric and Developmental Pathology
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