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https://www.readbyqxmd.com/read/28822194/-effect-of-oridonin-on-apoptosis-and-intracellular-reactive-oxygen-species-level-in-triple-negative-breast-cancer-mda-mb-231-cells
#1
Qi Qi, Pei Zhang, Qi-Xiang Li, Qiong Pan, Hai-Lun Zheng, Su-Rong Zhao
Oridonin, which is an ent-kaurene diterpenoid isolated from traditional Chinese medicine Rabdosia rubescens, displays various bioactivities, including anti-inflammation, anti-bacteria and anti-tumor. This study aimed to investigate the effect of oridonin on apoptosis of triple-negative breast cancer MDA-MB-231 cells and its underlying mechanisms. The inhibitory effect of oridonin on proliferation of MDA-MB-231 cells was measured by MTT assay; Apoptosis was analyzed by flow cytometry with PI staining and Annexin V-FITC/PI staining; Intracellular reactive oxygen species (ROS) level was determined by ROS detection kit, and expressions of PARP, Bcl-2, caspase-3 were analyzed by Western blot...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822191/-polydatin-induces-human-cervical-cancer-cell-apoptosis-via-pi3k-akt-mtor-signaling-pathway
#2
Ji-Hong Pan, Hai-Bin Wang, Xiao-Fei Du, Jiang-Yue Liu, Dai-Juan Zhang
To observe the effect of polydatin on proliferation and apoptosis of cervical cancer HeLa cells and explore its possible mechanism. The growth inhibitory effect was detected with MTT assay. After HeLa cells were treated with different concentrations (50, 100, 150 μmol•L⁻¹) of polydatin, MTT assay was used to detect the inhibitory effect of polydatin on proliferation of HeLa cells; Acridine orange/ethidium bromide staining was used for morphological changes in apoptotic HeLa cells; Annexin/propidium iodide staining was applied to detect HeLa cell apoptotic rate...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822186/-lignan-glycosides-and-sucrose-esters-from-roots-of-securidaca-inappendiculata
#3
Zhi Wang, Xue-Dong Yang, Hai-Yan Zha, Li-Na Guo, Dan Shen, Li-Zhen Xu, Shi-Lin Yang
Nine compounds, including five lignan glycosides (1-5), three sucrose esters (6-8), and one organic acid ester (9), were isolated from the ethanol extract of the roots of Securidaca inappendiculata by various chromatographic methods including silica gel, MPLC and preparative HPLC. Their structures were elucidated as acernikol-4″-O-β-D-glucopyranoside (1), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside (2), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside (3), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9'-O-β-D-glucopyranoside (4), (7R, 8S)-5-methoxydihydrodehy-drodiconiferyl alcohol 4-O-β-D-glucopyranoside (5), 3, 6'-O-diferuloylsucrose (6), 3-O-feruloyl-6'-O-sinapoylsucrose (7), sibricose A5 (8), and mehyl ferulate (9) on the basis of 1H-, 13C-NMR and MS experiments...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822143/-research-progress-of-rapid-detection-of-ochratoxin-a-and-its-application-prospect-in-traditional-chinese-medicine
#4
REVIEW
Shu-Rong Hu, Guang-Yao Ying, Yu-Li Hu, Shi-Hai Yang, Mei-Hua Yang
Ochratoxin A (OTA) is a toxic secondary metabolite mainly produced by Aspergillus and Penicillium species, with strong renal toxicity, teratogenic, carcinogenic, mutagenic effect. Studies have shown that OTA is not only widely contaminated in food and feed crops, but also has been widely contaminated in Chinese herbal medicines such as spices, licorice and so on. In view of OTA's universality and harmfulness, this paper summarizes the flow visualization test strip, microsphere, electrochemical sensor, surface enhanced Raman spectroscopy technology in OTA rapid detection, which provides reference for the research and application of high throughout detection instrument miniaturization in order to achieve OTA quick detection and simple operation...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28821913/molecular-insights-into-the-origin-of-the-brown-rust-resistance-gene-bru1-among-saccharum-species
#5
Heng-Bo Wang, Ping-Hua Chen, Yan-Qing Yang, Angelique D'Hont, Yun-Hai Lu
Analysis of 387 sugarcane clones using Bru 1 diagnostic markers revealed two possible sources of Bru 1 in Chinese cultivars: one from Saccharum spontaneum and another from Saccharum robustum of New Guinea. Sugarcane brown rust (SBR) is an important fungal disease in many sugarcane production areas around the world, and can cause considerable yield losses in susceptible sugarcane cultivars. One major SBR resistance gene, named Bru1, initially identified from cultivar R570, was shown to be a major SBR resistance source in most of the sugarcane producing areas of the world...
August 18, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28821559/synergy-of-wee1-and-mtor-inhibition-in-mutant-kras-driven-lung-cancers
#6
Josephine Hai, Shengwu Liu, Lauren Bufe, Khanh Do, Ting Chen, Xiaoen Wang, Christine Ng, Shuai Li, Ming-Sound Tsao, Geoffrey I Shapiro, Kwok-Kin Wong
Purpose:KRAS-activating mutations are the most common oncogenic driver in non-small cell lung cancer (NSCLC), but efforts to directly target mutant KRAS have proved a formidable challenge. Therefore, multi-targeted therapy may offer a plausible strategy to effectively treat KRAS-driven NSCLCs. Here, we evaluate the efficacy and mechanistic rationale for combining mTOR and WEE1 inhibition as a potential therapy for lung cancers harboring KRAS mutations. <p>Experimental Design: We investigated the synergistic effect of combining mTOR and WEE1 inhibitors on cell viability, apoptosis, and DNA damage repair response using a panel of human KRAS-mutant and wild type NSCLC cell lines and patient-derived xenograft cell lines...
August 18, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28820594/on-the-restrained-proton-indicator-in-combined-quantum-mechanics-molecular-mechanics-dynamics-simulations-of-proton-transfer-through-a-carbon-nanotube
#7
Adam W Duster, Hai Lin
Recently, a collective variable "proton indicator" was purposed for tracking an excess proton solvated in bulk water in molecular dynamics simulations. In this work, we demonstrate the feasibility of utilizing the position of this proton indicator as a reaction coordinate to model an excess proton migrating through a hydrophobic carbon nanotube in combined quantum-mechanics/molecular-mechanics simulations. Our results indicate that applying a harmonic restraint to the proton indicator in the bulk solvent near the nanotube pore entrance leads to the recruitment of water molecules into the pore...
August 18, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28820478/detection-of-interactions-between-proteins-by-using-legendre-moments-descriptor-to-extract-discriminatory-information-embedded-in-pssm
#8
Yan-Bin Wang, Zhu-Hong You, Li-Ping Li, Yu-An Huang, Hai-Cheng Yi
Protein-protein interactions (PPIs) play a very large part in most cellular processes. Although a great deal of research has been devoted to detecting PPIs through high-throughput technologies, these methods are clearly expensive and cumbersome. Compared with the traditional experimental methods, computational methods have attracted much attention because of their good performance in detecting PPIs. In our work, a novel computational method named as PCVM-LM is proposed which combines the probabilistic classification vector machine (PCVM) model and Legendre moments (LMs) to predict PPIs from amino acid sequences...
August 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28820468/resveratrol-in-hepatitis-c-patients-treated-with-pegylated-interferon-%C3%AE-2b-and-ribavirin-reduces-sleep-disturbance
#9
Manuela Pennisi, Gaetano Bertino, Caterina Gagliano, Michele Malaguarnera, Rita Bella, Antonio Maria Borzì, Roberto Madeddu, Filippo Drago, Giulia Malaguarnera
BACKGROUND: Hepatitis C virus infection and interferon treatment have shown to be risk factors for sleep disorder health-related quality of life. AIM: To determine whether the effects of resveratrol on sleep disorders were associated with different tests in subjects with chronic hepatitis C treated with Peg-IFN-α and RBV. PATIENTS AND METHODS: In this prospective, randomized, placebo controlled, double blind clinical trial, 30 subjects (Group A) with chronic hepatitis received Pegylated-Interferon-α2b (1...
August 18, 2017: Nutrients
https://www.readbyqxmd.com/read/28820366/determination-of-ferret-enteric-coronavirus-genome-in-laboratory-ferrets
#10
Tian-Cheng Li, Sayaka Yoshizaki, Michiyo Kataoka, Yen Hai Doan, Yasushi Ami, Yuriko Suzaki, Tomofumi Nakamura, Naokazu Takeda, Takaji Wakita
Ferret enteric coronavirus (FRECV) RNA was detected in laboratory ferrets. Analysis of the complete genome sequence of 2 strains, FRCoV4370 and FRCoV063, revealed that FRECV shared 49.9%-68.9% nucleotide sequence identity with known coronaviruses. These results suggest that FRECV might be classified as a new species in the genus Alphacoronavirus.
September 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28820124/bacillus-wudalianchiensis-sp-nov-isolated-from-grass-soils-of-the-wudalianchi-scenic-area
#11
Bo Liu, Guo-Hong Liu, Cetin Sengonca, Peter Schumann, Jie-Ping Wang, Yu-Jing Zhu, Hai-Feng Zhang
A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-27215T, was isolated from grass soil collected from Wudalianchi in the Heilongjiang Province of China. Growth was observed at 10-60 °C (optimum 30 °C), in 0 and 3.0 % NaCl (optimum 0 %) and at pH 5.0-10.0 (optimum 7.0), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK7. The main fatty acids were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0, and iso-C16 : 0...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28819994/the-mechanistic-antitumor-study-of-myricanol-5-fluorobenzyloxy-ether-in-human-leukemic-cell-hl-60
#12
Guan-Hai Dai, Chen-Jie Fan, Ze-Ming Ren, Xuan Chen, Ye-Ling Tong, Zhen-Hua Li, Xiao-Jing Nie, Ke-Qun Chai
AIM: The aim of the study was to explore the growth inhibitory effect of myricanol 5-fluorobenzyloxy ether (5FEM) and the underlying mechanism in human leukemic cells HL-60. MATERIALS & METHODS: 5FEM was obtained by chemical modification of myricanol with fluorobenzyloxy ether at the OH(5) position. The cytotoxicity, cell apoptosis, cell cycle and the expression of key apoptosis-related genes in HL-60 were evaluated. RESULTS & CONCLUSION: 5FEM can significantly inhibited growth of HL-60 cells, increased the G2/M population and upregulated the expression of Bax, Fas, FasL, caspase-9 and p21 and downregulated that of Bcl-2 and survivin...
August 18, 2017: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/28819980/superior-fluorogen-activating-protein-probes-based-on-3-indole-malachite-green
#13
Qingyang Zhang, Qinghua Wang, Ying Sun, Limin Zuo, Verena Fetz, Hai-Yu Hu
A series of novel fluorogenic dyes based on 3-indole-Malachite Green, MGs 5-7, have been developed that are dark in solution but highly fluorescent when bound to the cognate reporter, fluorogen-activating protein (FAP). Significantly, the new MGs 5-7 probes are superior to the traditional MG 1 with high fluorescent efficiency and low toxicity to cells while maintaining the large "pseudo-Stokes" shifts (Δλ = λex - λem) and the malachite green (MG)-like fluorescence OFF-ON switching mechanism in both live mammalian cells and bacterial cells when bound to FAP...
August 18, 2017: Organic Letters
https://www.readbyqxmd.com/read/28819779/curcuma-wenyujin-y-h-chen-et-c-ling-n-butyl-alcohol-extract-inhibits-ags-cell-helicobacter-pylori-caga-vaca-promoted-invasiveness-by-down-regulating-caudal-type-homeobox-transcription-factor-and-claudin-2-expression
#14
Hai-Feng Jin, Jin-Feng Dai, Li-Na Meng, Bin Lu
OBJECTIVE: To investigate the effects and possible mechanisms of action of Curcuma wenyujin Y. H. Chen et C. Ling n-Butyl alcohol extract (CWNAE) on repression of human gastric cancer (GC) AGS cell invasion induced by co-culturing with Helicobacter pylori (HP). METHODS: AGS cells were cultured with HP of positive or negative cytotoxin-associated gene A (CagA) and vacuolating cytotoxin gene A (VacA) expression (CagA+/- or VacA+/-) and divided into 5 group. Group A was cultured without HP as a control, Group B with HPCagA+VacA+, Group C with HPCagA-VacA-, Group D with HPCagA+VacA+ and CWNAE, and Group E with HPCagA-VacA- and CWNAE...
August 17, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28819684/comparison-of-transcriptome-profiles-by-fusarium-oxysporum-inoculation-between-fusarium-yellows-resistant-and-susceptible-lines-in-brassica-rapa-l
#15
Naomi Miyaji, Motoki Shimizu, Junji Miyazaki, Kenji Osabe, Maho Sato, Yusuke Ebe, Satoko Takada, Makoto Kaji, Elizabeth S Dennis, Ryo Fujimoto, Keiichi Okazaki
Resistant and susceptible lines in Brassica rapa have different immune responses against Fusarium oxysporum inoculation. Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to Foc is still unknown. By comparing with and without Foc inoculation in both resistant and susceptible lines of Chinese cabbage (Brassica rapa var. pekinensis), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples...
August 17, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28819451/a-high-affinity-repebody-for-molecular-imaging-of-egfr-expressing-malignant-tumors
#16
Misun Yun, Dong-Yeon Kim, Joong-Jae Lee, Hyeon-Sik Kim, Hyung-Seok Kim, Ayoung Pyo, Yiseul Ryu, Tae-Yoon Kim, Jin Hai Zheng, Su Woong Yoo, Hoon Hyun, Gyungseok Oh, Jaeho Jeong, Myeongju Moon, Jung-Hyun Min, Seong Young Kwon, Jung Young Kim, Euiheon Chung, Yeongjin Hong, Wansik Lee, Hak-Sung Kim, Jung-Joon Min
The accurate detection of disease-related biomarkers is crucial for the early diagnosis and management of disease in personalized medicine. Here, we present a molecular imaging of human epidermal growth factor receptor (EGFR)-expressing malignant tumors using an EGFR-specific repebody composed of leucine-rich repeat (LRR) modules. The repebody was labeled with either a fluorescent dye or radioisotope, and used for imaging of EGFR-expressing malignant tumors using an optical method and positron emission tomography...
2017: Theranostics
https://www.readbyqxmd.com/read/28819426/central-role-of-cemip-in-tumorigenesis-and-its-potential-as-therapeutic-target
#17
REVIEW
Li Li, Lin-Hai Yan, Shwetha Manoj, Ying Li, Lu Lu
CEMIP (KIAA1199) was identified as migratory indicator protein which had been crudely studied in the last decade. Firstly its mutation site was reported to cause hearing loss due to the folding change of protein structure, meanwhile the over-expression of CEMIP referred to dreadful invasion and uncontrolled proliferation of tumor with distant metastasis, dedifferentiation, and limited survival opportunity of patients. Especially, over-expressed CEMIP also protected malignant tumor from strict microenvironment in hypoxia, low glucose and cracked barrier, leading to enhanced adaptability of tumor by stimulating the Wnt, EGFR, FGFR pathway...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28819375/bone-marrow-derived-mesenchymal-stem-cells-involve-in-the-lymphangiogenesis-of-lung-cancer-and-jinfukang-inhibits-the-involvement-in-vivo
#18
Xian-Mei Zhou, Dan Wang, Hai-Lang He, Jie Tang, Jing Wu, Ling Xu, Jian-Xin Li
Lymphangiogenesis plays an important role in cancer metastasis. Bone marrow-derived mesenchymal stem cells (BMMSCs) migrate to the site of tumorigenesis and in turn promote the metastasis. However, whether BMMSCs involve in the lymphangiogenesis of lung cancer is unclear. Jinfukang has clinically been used for the treatment of non small cell lung cancer (NSCLC) in China. In this study, to investigate the involvement of BMMSCs in lymphangiogenesis in lung cancer, and evaluate the inhibitory effect of Jinfukang on the lymphangiogenesis, chimeric mice were prepared by transplanting bone marrow from green fluorescent protein (GFP) transgenic mice (C57BL/6-EGFP) into irradiated C57BL/6 mice...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28819147/nitrogen-starvation-impacts-the-photosynthetic-performance-of-porphyridium-cruentum-as-revealed-by-chlorophyll-a-fluorescence
#19
Long-Sheng Zhao, Kang Li, Qian-Min Wang, Xiao-Yan Song, Hai-Nan Su, Bin-Bin Xie, Xi-Ying Zhang, Feng Huang, Xiu-Lan Chen, Bai-Cheng Zhou, Yu-Zhong Zhang
Nitrogen is one of the most important nutrients needed for plants and algae to survive, and the photosynthetic ability of algae is related to nitrogen abundance. Red algae are unique photosynthetic eukaryotic organisms in the evolution of algae, as they contain phycobilisomes (PBSs) on their thylakoid membranes. In this report, the in vivo chlorophyll (Chl) a fluorescence kinetics of nitrogen-starved Porphyridium cruentum were analyzed to determine the effects of nitrogen deficiency on photosynthetic performance using a multi-color pulse amplitude modulation (PAM) chlorophyll fluorometer...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819143/immunomodulatory-and-antioxidant-effects-of-total-flavonoids-of-spatholobus-suberectus-dunn-on-pcv2-infected-mice
#20
Yuan-Fang Fu, Li-He Jiang, Wei-Dan Zhao, Meng Xi-Nan, Shi-Qi Huang, Jian Yang, Ting-Jun Hu, Hai-Lan Chen
Oxidative stress plays an important role in the pathogenesis of virus infection and antioxidants are becoming promising candidates as therapeutic agents. This study is designed to investigate the effect of total flavonoids of Spatholobus suberectus Dunn (TFSD) on oxidative stress in mice induced by porcine circovirus type 2 (PCV2) infection. The PCV2 infection leads to significant decrease in thymus and spleen indices, elevation of xanthine oxidase (XOD) and myeloperoxidase (MPO) activities, reduction in GSH level and GSH to GSSG ratio and decline of superoxide dismutase (SOD) activity, indicating the formation of immunosuppression and oxidative stress...
August 17, 2017: Scientific Reports
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