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https://www.readbyqxmd.com/read/30106161/microrna%C3%A2-34b-promotes-proliferation-migration-and-invasion-of-ewing-s-sarcoma-cells-by-downregulating-notch1
#1
Qunshan Lu, Mei Lu, Dong Li, Shuai Zhang
Ewing's sarcoma is the second most frequent bone and soft tissue sarcoma, which is commonly driven by the Ewing's sarcoma breakpoint region 1‑friend leukemia integration 1 transcription factor (EWS‑FLI1) fusion gene. Since microRNAs (miRs) can act as either oncogenes or tumor suppressor genes in human cancer, and miR‑34b has been reported to act as a tumor suppressor, the role of miR‑34b in Ewing's sarcoma was investigated in the present study. The results demonstrated that miR‑34b expression levels were higher in tumor samples compared within normal tissue samples...
August 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/30100545/tricellulin-expression-and-its-deletion-effects-in-the-endolymphatic-sac
#2
Ai Matsubara, Takenori Miyashita, Ryuhei Inamoto, Hirofumi Sakaguchi, Toru Kamitani, Nozomu Mori, Hiroshi Hoshikawa
OBJECTIVES: Tricellulin is a tight junction (TJ)-forming protein that participates in the sealing function of tricellular TJs. Tricellulin-knockout (Tric-/-) mice show progressive hearing loss with degeneration of hair cells in the cochlea without physiological or physical disorders. In the present study, we investigated the tricellulin expression and its deletion effects in the endolymphatic sac (ES) using Tric-/- mice. MATERIALS AND METHODS: The ES epithelia from wild-type (WT) mice were laser-microdissected, and RT-PCR was performed...
August 3, 2018: Journal of International Advanced Otology
https://www.readbyqxmd.com/read/30098570/optimal-electrical-stimulation-boosts-stem-cell-therapy-in-nerve-regeneration
#3
Jian Du, Gehua Zhen, Huanwen Chen, Shuming Zhang, Liming Qing, Xiuli Yang, Gabsang Lee, Hai-Quan Mao, Xiaofeng Jia
Peripheral nerve injuries often lead to incomplete recovery and contribute to significant disability to approximately 360,000 people in the USA each year. Stem cell therapy holds significant promise for peripheral nerve regeneration, but maintenance of stem cell viability and differentiation potential in vivo are still major obstacles for translation. Using a made-in-house 96-well vertical electrical stimulation (ES) platform, we investigated the effects of different stimulating pulse frequency, duration and field direction on human neural crest stem cell (NCSC) differentiation...
July 20, 2018: Biomaterials
https://www.readbyqxmd.com/read/30097029/irinotecan-platinum-combination-therapy-for-previously-untreated-extensive-stage-small-cell-lung-cancer-patients-a-meta-analysis
#4
Fei Xu, Xiaoli Ren, Yuan Chen, Qianxia Li, Ruichao Li, Yu Chen, Shu Xia
BACKGROUND: There is still a debate regarding whether regimens combining irinotecan with platinum could replace regimens combining etoposide with platinum, as first-line chemotherapy for extensive-stage small cell lung cancer (ES-SCLC). We performed a meta-analysis to compare these regimens as first-line chemotherapy for ES-SCLC. METHODS: A literature search for randomized controlled trials was performed using the Cochrane Library, PubMed, and Embase. The inverse variance method was used to estimate summary hazard ratios and their 95% confidence intervals for overall survival and progression free survival...
August 10, 2018: BMC Cancer
https://www.readbyqxmd.com/read/30091663/lower-cutaneous-microvascular-reactivity-in-adult-cancer-patients-receiving-chemotherapy
#5
Shelbi L Sutterfield, Jacob T Caldwell, Hunter K Post, Garrett M Lovoy, Heather R Banister, Carl J Ade
Cancer patients with a history of anti-cancer chemotherapy are at an increased cardiovascular disease risk compared to the cancer free populations. Therefore, we tested the hypothesis that cancer patients receiving adjuvant chemotherapy would have a lower cutaneous microvascular reactivity and lower endothelium-dependent flow-mediated dilation of the brachial artery compared to matched cancer-free controls. To test this hypothesis, we performed a case control study using seven cancer patients receiving adjuvant chemotherapy and seven matched healthy reference controls...
August 9, 2018: Journal of Applied Physiology
https://www.readbyqxmd.com/read/30091053/lack-of-effective-translational-regulation-of-pld-expression-and-exosome-biogenesis-in-triple-negative-breast-cancer-cells
#6
Julian Gomez-Cambronero
Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer that is difficult to treat since cells lack the three receptors (ES, PR, or HER) that the most effective treatments target. We have used a well-established TNBC cell line (MDA-MB-231) from which we found evidence in support for a phospholipase D (PLD)-mediated tumor growth and metastasis: high levels of expression of PLD, as well as the absence of inhibitory miRs (such as miR-203) and 3'-mRNA PARN deadenylase activity in these cells...
August 8, 2018: Cancer Metastasis Reviews
https://www.readbyqxmd.com/read/30089752/chimeric-analysis-with-newly-established-egfp-dsred2-tagged-es-cells-identify-hydin-as-essential-for-spermiogenesis-in-mice
#7
Seiya Oura, Haruhiko Miyata, Taichi Noda, Keisuke Shimada, Takafumi Matsumura, Akane Morohoshi, Ayako Isotani, Masahito Ikawa
The CRISPR/Cas9 system can efficiently introduce biallelic mutations in ES cells (ESCs), and its application with fluorescently-tagged ESCs enables phenotype analysis in chimeric mice. We have utilized ESCs that express EGFP in the cytosol and acrosome [EGR-G101 129S2 x (CAG/Acr-EGFP) B6] in previous studies; however, the EGFP signal in the sperm cytosol is weak and the signal in the acrosome is lost after the acrosome reaction, precluding analysis between wild type and ESC derived spermatozoa. In this study, we established an ESC line from RBGS (Red Body Green Sperm) transgenic mice [B6D2-Tg(CAG/Su9-DsRed2, Acr3-EGFP)RBGS002Osb] whose spermatozoa exhibit green fluorescence in the acrosome and red fluorescence in the mitochondria within the flagellar midpiece that is retained after the acrosome reaction...
August 9, 2018: Experimental Animals
https://www.readbyqxmd.com/read/30089587/eortc-qlq-c30-summary-score-reliably-detects-changes-in-qol-three-months-after-anatomic-lung-resection-for-non-small-cell-lung-cancer-nsclc
#8
Cecilia Pompili, Michael Koller, Galina Velikova, Kevin Franks, Kate Absolom, Matthew Callister, Jonathan Robson, Andrea Imperatori, Alessandro Brunelli
INTRODUCTION: We tested the European Organization for Research and Treatment of Cancer Quality of Life (EORTC QLQ-C30) summary score (SumSC) to detect changes in the HRQOL after Non-small-cell lung cancer (NSCLC) surgery and compared its performance to the traditional scales. METHOD: EORTC QLQ-C30 data was obtained from 326 consecutive pre-operative patients submitted for anatomical lung resections for NSCLC.66 patients completed post-operative assessments 3 months after surgery...
September 2018: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
https://www.readbyqxmd.com/read/30084812/meta-analysis-of-gene-expression-profiles-of-peripheral-blood-cells-in-systemic-lupus-erythematosus
#9
Sang-Cheol Bae, Young Ho Lee
Our purpose was to identify differentially expressed (DE) genes and biological processes associated with gene expression changes in systemic lupus erythematosus (SLE). We performed a meta-analysis using the INMEX program (integrative meta-analysis of expression data) on publicly available microarray Genetic Expression Omnibus (GEO) datasets of peripheral blood mononuclear cells (PBMCs) of SLE patients and healthy controls. We performed Gene Ontology (GO) enrichment analysis by using hypergeometric tests. In total, five comparisons (2 B cells, 2 CD4 T cells, and 1 myeloid cell) from two GEO datasets containing 51 cases and 46 controls were included in the meta-analysis...
July 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30080404/an-affinity-based-probe-for-the-human-adenosine-a-2a-receptor
#10
Xue Yang, Thomas J M Michiels, Coen de Jong, Marjolein Soethoudt, Niek Dekker, Euan Gordon, Mario van der Stelt, Laura H Heitman, Daan van der Es, Adriaan P IJzerman
Using activity-based protein profiling (ABPP) functional proteins can be interrogated in their native environment. Despite their pharmaceutical relevance G protein-coupled receptors (GPCRs), have been difficult to address through ABPP. In the current study, we took the prototypical human adenosine A2A receptor (hA2A R) as the starting point for the construction of a chemical toolbox allowing two-step affinity-based labeling of GPCRs. Firstly, we equipped an irreversibly binding hA2A R ligand with a terminal alkyne to serve as probe...
August 6, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/30080396/cell-cycle-dependent-uptake-and-cytotoxicity-of-arsenic-based-drugs-in-single-leukemia-cells
#11
Ying Zhou, Haibo Wang, Eric Tse, Hongyan Li, Hongzhe Sun
Arsenic has long been used as therapeutic agents. Understanding the mechanism of action of arsenic-based drugs enables more ef-fective arsenic drugs to be developed. Cell cycle has been known to play a critical role for cell division and growth. Herein, we es-tablish a methodology to evaluate the uptake of two arsenic-based drugs (ATO and ZIO-101) across the cell cycle in single leukemia cells, i.e., NB4 and HL60, using a double thymidine block combined with time-resolved ICP-MS. We show that cells absorb maxi-mal amounts of both ATO and ZIO-101 in G2/M phase and minimum in S phase, and such variation is less apparent for ZIO-101 than ATO (NB4-G2/M: S=2...
August 6, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30078226/effects-of-biphasic-and-monophasic-electrical-stimulation-on-mitochondrial-dynamics-cell-apoptosis-and-cell-proliferation
#12
Maria R Love, Jirapas Sripetchwandee, Siripong Palee, Siriporn C Chattipakorn, Morton M Mower, Nipon Chattipakorn
Currently, electrical stimulation (ES) is used to induce changes in various tissues and cellular processes, but its effects on mitochondrial dynamics and mechanisms are unknown. The aim of this study was to compare the effects of monophasic and biphasic, anodal, and cathodal ES on apoptosis, proliferation, and mitochondrial dynamics in neuroblastoma SH-SY5Y cells. Cells were cultured and treated with ES. Alamar blue assay was performed to measure cell proliferation. The proteins expression of apoptotic-related proteins Bcl-2 associated X (Bax), B cell lymphoma 2 (Bcl-2), optic-atrophy-1 (OPA1), mitofusin2 (Mfn2), phosphorylated dynamin-related protein 1 at serine 616 (p-DRP1), and total dynamin-related protein 1 (Total-DRP1) were also determined...
August 4, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30078102/application-of-pluripotent-stem-cells-for-treatment-of-human-neuroendocrine-disorders
#13
REVIEW
Hidetaka Suga
The neuroendocrine system is composed of many types of functional cells. Matured cells are generally irreversible to progenitor cells and it is difficult to obtain enough from our body. Therefore, studying specific subtypes of human neuroendocrine cells in vitro has not been feasible. One of the solutions is pluripotent stem cells, such as embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. These are unlimited sources and, in theory, are able to give rise to all cell types of our body. Therefore, we can use them for regenerative medicine, developmental basic research and disease modeling...
August 4, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/30074587/the-role-of-the-basement-plate-in-physeal-bar-formation
#14
John Michael Wattenbarger, Amanda Marshall, Michael D Cox, Helen Gruber
BACKGROUND: Physeal fractures and resultant physeal bars can pose significant problems in skeletal development for the injured growing child. Although now well-recognized, only a small body of experimental literature covering this problem is available. The goal of this study was to help further develop an understanding of the different regions of the physis and the way in which each region responds to injury/fracture. METHODS: This Institutional Animal Care and Use Committee (IACUC)-approved study assessed bar formation using radiologic and histologic methods and measured leg lengths of skeletally immature rats...
August 1, 2018: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/30067731/-incisi%C3%A3-n-cervical-transversa-en-disecci%C3%A3-n-radical-de-cuello
#15
José Francisco Gallegos-Hernández
Antecedentes: La disección radical de cuello es la única forma de estadificar a pacientes con neoplasias con riesgo de metástasis ganglionares. Se han efectuado diversos tipos de incisiones a lo largo de la historia, con el objetivo de obtener una exposición suficiente que permita la resección completa de los grupos ganglionares en riesgo. Es importante combinar la seguridad oncológica con unas adecuadas estética, funcionalidad y calidad de vida. Método: Evaluación retrospectiva del resultado obtenido con la incisión transversa en el cuello en pacientes sometidos a disección radical...
2018: Cirugia y Cirujanos
https://www.readbyqxmd.com/read/30065644/hsa-let-7c-mirna-regulates-synaptic-and-neuronal-function-in-human-neurons
#16
Heather McGowan, Vincent R Mirabella, Aula Hamod, Aziz Karakhanyan, Nicole Mlynaryk, Jennifer C Moore, Jay A Tischfield, Ronald P Hart, Zhiping P Pang
Non-coding RNA, including microRNA (miRNA) serves critical regulatory functions in the developing brain. The let-7 family of miRNAs has been shown to regulate neuronal differentiation, neural subtype specification, and synapse formation in animal models. However, the regulatory role of human let-7c (hsa-let-7c) in human neuronal development has yet to be examined. Let-7c is encoded on chromosome 21 in humans and therefore may be overexpressed in human brains in Trisomy 21 (T21), a complex neurodevelopmental disorder...
2018: Frontiers in Synaptic Neuroscience
https://www.readbyqxmd.com/read/30060666/sulfonamido-2-arylbenzoxazole-groel-es-inhibitors-are-potent-antibacterials-against-methicillin-resistant-staphylococcus-aureus-mrsa
#17
Sanofar Abdeen, Trent Kunkle, Nilshad Salim, Anne-Marie Ray, Najiba Mammadova, Corey Summers, Mckayla Stevens, Andrew Ambrose, Yangshin Park, Peter G Schultz, Arthur L Horwich, Quyen Hoang, Eli Chapman, Steven M Johnson
Extending from a study we recently published examining the anti-trypanosomal effects of a series of GroEL/ES inhibitors based on a pseudo-symmetrical bis-sulfonamido-2-phenylbenzoxazole scaffold, here, we report the antibiotic effects of asymmetric analogs of this scaffold against a panel of bacteria known as the ESKAPE pathogens ( Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species). While GroEL/ES inhibitors were largely ineffective against K...
July 30, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/30056461/cytotoxicity-assessment-of-1-peracetic-acid-2-5-sodium-hypochlorite-and-17-edta-on-fg11-and-fg15-human-fibroblasts
#18
Pedro A Teixeira, Marcelo S Coelho, Augusto S Kato, Carlos E Fontana, Carlos Es Bueno, Daniel G Pedro-Rocha
The aim of this study was to evaluate the cytotoxic effects of 2. 5% sodium hypochlorite (NaOCl), 17% ethylenediamine tetraacetic acid (EDTA), and 1% peracetic acid (PAA) on human fibroblasts. FG11 and FG15 cell lines were cultured in 24-well cell culture plates for cell proliferation assessment and 96-well cell culture plates for the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay; Dulbecco's modified Eagle's medium (DMEM) was used as control data. The experimental solutions were used at 0. 01%, 0...
June 2018: Acta Odontológica Latinoamericana: AOL
https://www.readbyqxmd.com/read/30053872/emerging-roles-of-myc-in-stem-cell-biology-and-novel-tumor-therapies
#19
REVIEW
Go J Yoshida
The pathophysiological roles and the therapeutic potentials of Myc family are reviewed in this article. The physiological functions and molecular machineries in stem cells, including embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, are clearly described. The c-Myc/Max complex inhibits the ectopic differentiation of both types of artificial stem cells. Whereas c-Myc plays a fundamental role as a "double-edged sword" promoting both iPS cells generation and malignant transformation, L-Myc contributes to the nuclear reprogramming with the significant down-regulation of differentiation-associated genetic expression...
July 27, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/30052778/gpseudorank-a-permutation-sampler-for-single-cell-orderings
#20
Magdalena E Strauß, John E Reid, Lorenz Wernisch
Motivation: A number of pseudotime methods have provided point estimates of the ordering of cells for scRNA-seq data. A still limited number of methods also model the uncertainty of the pseudotime estimate. However, there is still a need for a method to sample from complicated and multi-modal distributions of orders, and to estimate changes in the amount of the uncertainty of the order during the course of a biological development, as this can support the selection of suitable cells for the clustering of genes or for network inference...
July 25, 2018: Bioinformatics
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