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https://www.readbyqxmd.com/read/30317699/role-of-pyrimidine-salvage-pathway-in-the-maintenance-of-the-organellar-and-nuclear-genome-integrity
#1
Pedroza-García José Antonio, Nájera-Martínez Manuela, Mazubert Christelle, Aguilera-Alvarado Paulina, Drouin-Wahbi Jeannine, Sánchez-Nieto Sobeida, José M Gualberto, Raynaud Cécile, Plasencia Javier
Nucleotide biosynthesis proceeds through a de novo pathway and a salvage route. In the latter, free bases and/or nucleosides are recycled to generate the corresponding nucleotides. Thymidine kinase (TK) is the first enzyme in the salvage pathway to recycle thymidine nucleosides as it phosphorylates thymidine to yield thymidine monophosphate. The Arabidopsis genome contains two TK genes -TK1a and TK1b- that show similar expression patterns during development. In this work, we studied the respective roles of the two genes during early development and in response to genotoxic agents targeting the organellar or the nuclear genome...
October 13, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/30317637/brain-derived-neurotrophic-factor-alleviates-the-oxidative-stress-induced-by-oxygen-and-glucose-deprivation-in-an-ex-vivo-brain-slice-model
#2
Paloma González-Rodríguez, Irene F Ugidos, Diego Pérez-Rodríguez, Berta Anuncibay-Soto, María Santos-Galdiano, Enrique Font-Belmonte, José Manuel Gonzalo-Orden, Arsenio Fernández-López
Brain-derived neurotrophic factor (BDNF) is considered as a putative therapeutic agent against stroke. Since BDNF role on oxidative stress is uncertain, we have studied this role in a rat brain slice ischemia model, which allows BDNF reaching the neural parenchyma. Hippocampal and cerebral cortex slices were subjected to oxygen and glucose deprivation (OGD) and then returned to normoxic conditions (reperfusion-like, RL). OGD/RL increased a number of parameters mirroring oxidative stress in the hippocampus that were reduced by the BDNF presence...
October 14, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30317478/expression-purification-and-activity-analysis-of-chlorophyllide-oxidoreductase-and-ni-2-sirohydrochlorin-a-c-diamide-reductase
#3
Jürgen Moser, Jan Jasper, José Vazquez Ramos, Sven T Sowa, Gunhild Layer
Nitrogenase-like enzymes play a vital role in the reduction of the conjugated ring systems of diverse tetrapyrrole molecules. The biosynthesis of all bacteriochlorophylls involves the two-electron reduction of the C7-C8 double bond of the green pigment chlorophyllide, which is catalyzed by the nitrogenase-like two-component metalloenzyme chlorophyllide oxidoreductase (COR); whereas in all methanogenic microbes, another nitrogenase-like system, CfbC/D, is responsible for the sophisticated six-electron reduction of Ni2+ -sirohydrochlorin a,c-diamide in the course of coenzyme F430 biosynthesis...
2019: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30317421/diallyl-trisulfide-protects-motor-neurons-from-the-neurotoxic-protein-tdp-43-via-activating-lysosomal-degradation-and-the-antioxidant-response
#4
Chang Liu, Bingquan Leng, Yi Li, Hong Jiang, Weisong Duan, Yansu Guo, Chunyan Li, Kun Hong
Amyotrophic lateral sclerosis (ALS) is a rapidly progressive motor neuron disease for which only limited effective therapeutics are available. Currently, TAR DNA-binding protein 43 (TDP-43) is recognized as a pathological and biochemical marker for ALS. Increases in the levels of aggregated or mislocalized forms of TDP-43 might result in ALS pathology. Therefore, clearance pathways for intracellular protein aggregates have been suggested as potential therapeutic targets for the treatment of ALS. Here we report that treatment of motor neuron-like NSC34 cells overexpressing TDP-43 with diallyl trisulfide (DATS) induced neuronal autophagy and lysosomal clearance of TDP-43 and C-terminal TDP-43 fragments...
October 13, 2018: Neurochemical Research
https://www.readbyqxmd.com/read/30315637/activin-a-target-genes-are-differentially-expressed-between-normal-and-neoplastic-adult-human-testes-clues-to-gonocyte-fate-choice
#5
M Szarek, M Bergmann, L Konrad, H-C Schuppe, S Kliesch, M P Hedger, K L Loveland
BACKGROUND: Human testicular germ cell tumours (TGCT) arise from germ cell neoplasia in situ (GCNIS) cells that originate from foetal germ cell precursors. Activin A is central to normal foetal testis development, and its dysregulation may contribute to TGCT aetiology. OBJECTIVE: (i) To test whether the expression profiles of activin A targets in normal and neoplastic human testes indicates functional links with TGCT progression. (ii) To investigate whether activin A levels influence MMP activity in a neoplastic germ cell line...
October 13, 2018: Andrology
https://www.readbyqxmd.com/read/30315602/effects-of-l-methionine-on-performance-gut-morphology-and-antioxidant-status-in-gut-and-liver-of-piglets-in-relation-to-dl-methionine
#6
Johanna O Zeitz, Susanne Kaltenböck, Erika Most, Klaus Eder
This study investigated the hypothesis that dietary supplementation of L-methionine (L-Met) in weaned piglets in relation to DL-methionine (DL-Met) results in a higher antioxidant status and lower need for antioxidant enzyme activation in intestinal epithelium and body tissues, and improves gut morphology and gut barrier function as well as performance. A total of 99 early-weaned 21-day old piglets were allotted to six groups and fed a semi-synthetic wheat-barley-based basal diet supplemented with 0.067%, 0...
October 12, 2018: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/30315106/the-mucinous-domain-of-pancreatic-carboxyl-ester-lipase-cel-contains-core-1-core-2-o-glycans-that-can-be-modified-by-abo-blood-group-determinants
#7
Khadija El Jellas, Bente B Johansson, Karianne Fjeld, Aristotelis Antonopoulos, Heike Immervoll, Man H Choi, Dag Hoem, Mark E Lowe, Dominique Lombardo, Pål R Njølstad, Anne Dell, Eric Mas, Stuart M Haslam, Anders Molven
Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease. Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis. Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP). The monoclonal antibody mAb16D10 was previously reported to detect a glycotope in the highly O-glycosylated, mucin-like C-terminus of CEL/FAPP...
October 12, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30315077/carbaryl-as-carbon-and-nitrogen-source-characterization-of-inducible-methylamine-metabolic-pathway-at-the-biochemical-and-molecular-level-in-pseudomonas-sp-c5pp
#8
Kamini, Rakesh Sharma, Narayan S Punekar, Prashant S Phale
Carbaryl is the most widely used carbamate family pesticide whose persistent nature pollutes both soil and water ecosystems. Microbes maintain the earth's bio-geochemical cycles by metabolizing various compounds present in the matter including xenobiotic as a sole source of carbon, nitrogen, and energy. Soil isolate Pseudomonas sp. C5pp metabolizes carbaryl efficiently as the carbon source. Periplasmic carbaryl hydrolase catalyzes the conversion of carbaryl to 1-naphthol and methylamine. 1-Naphthol was further used as carbon source via gentisate whereas the metabolic fate of methylamine is not known...
October 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/30314694/identification-of-rubisco-anxiolytic-like-peptides-ralps-by-comprehensive-analysis-of-spinach-green-leaf-protein-digest
#9
Saeko Kimura, Tomoki Uchida, Yuki Tokuyama, Mayumi Hosokawa, Junya Nakato, Atsushi Kurabayashi, Masaru Sato, Hideyuki Suzuki, Ryuhei Kanamoto, Kousaku Ohinata
Rubisco, an enzyme for photosynthetic carbon dioxide fixation, is a major green leaf protein and known as the most abundant protein on the Earth. We found that Rubisco digested mimicking gastrointestinal enzymatic conditions exhibited anxiolytic-like effects after oral administration in mice. Based on a comprehensive peptide analysis of the digest using nanoLC-Orbitrap-MS and the structure-activity relationship of known anxiolytic-like peptides, we identified SYLPPLTT, SYLPPLT and YHIEPV [termed Rubisco anxiolytic-like peptide (rALP)-1, rALP-1(1-7) and rALP-2, respectively], which exhibited potent anxiolytic-like effects after oral administration...
October 9, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30312968/a-stress-associated-protein-lmsap-from-the-halophyte-lobularia-maritima-provides-tolerance-to-heavy-metals-in-tobacco-through-increased-ros-scavenging-and-metal-detoxification-processes
#10
Rania Ben Saad, Anis Ben Hsouna, Walid Saibi, Karim Ben Hamed, Faical Brini, Thaura Ghneim-Herrera
Agricultural soil pollution by heavy metals is a severe global ecological problem. We recently showed that overexpression of LmSAP, a member of the stress-associated protein (SAP) gene family isolated from Lobularia maritima, in transgenic tobacco led to enhanced tolerance to abiotic stress. In this study, we characterised the response of LmSAP transgenic tobacco plants to metal stresses (cadmium (Cd), copper (Cu), manganese (Mn), and zinc (Zn)). In L. maritima, LmSAP expression increased after 12 h of treatment with these metals, suggesting its involvement in the plant response to heavy metal stress...
September 29, 2018: Journal of Plant Physiology
https://www.readbyqxmd.com/read/30312933/in-silico-characterization-and-transcriptional-modulation-of-phenylalanine-ammonia-lyase-pal-by-abiotic-stresses-in-the-medicinal-orchid-vanda-coerulea-griff-ex-lindl
#11
Swagata Nag, Suman Kumaria
Phenylalanine ammonia lyase (PAL) is the first enzyme of phenylpropanoid pathway. In the present study, a full-length PAL transcript from Vanda coerulea Griff. ex Lindl. (Family: Orchidaceae) was isolated and characterized. It was found that complete PAL transcript of V. coerulea (VcPAL; Gene Bank no. MG745168) contained 2175 bp with the open reading frame (ORF) of 2112 bp, encoding 703 amino acid residues. The multiple sequence alignment showed that VcPAL protein had 81% identity with that of the orchid, Bromheadia finlaysoniana...
October 9, 2018: Phytochemistry
https://www.readbyqxmd.com/read/30312700/artificial-chaperones-based-on-thermoresponsive-polymers-recognize-the-unfolded-state-of-the-protein
#12
Pavel Semenyuk, Tony Tiainen, Sami Hietala, Heikki Tenhu, Vladimir Aseyev, Vladimir Muronetz
Stabilization of the enzymes under stress conditions is of special interest for modern biochemistry, bioengineering, as well as for formulation and target delivery of protein-based drugs. Aiming to achieve an efficient stabilization at elevated temperature with no influence on the enzyme under normal conditions, we studied chaperone-like activity of thermoresponsive polymers based on poly(dimethylaminoethyl methacrylate) (PDMAEMA) toward two different proteins, glyceraldehyde-3-phosphate dehydrogenase and chicken egg lysozyme...
October 9, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/30312667/a-microglial-cell-model-for-acyl-coa-oxidase-1-deficiency
#13
Q Raas, F-E Saih, C Gondcaille, D Trompier, Y Hamon, V Leoni, C Caccia, B Nasser, M Jadot, F Ménétrier, G Lizard, M Cherkaoui-Malki, P Andreoletti, S Savary
Acyl-CoA oxidase (ACOX1) deficiency is a rare and severe peroxisomal leukodystrophy associated with a very long-chain fatty acid (VLCFA) β-oxidation defect. This neurodegenerative disease lacks relevant cell models to further decipher the pathomechanisms in order to identify novel therapeutic targets. Since peroxisomal defects in microglia appear to be a key component of peroxisomal leukodystrophies, we targeted the Acox1 gene in the murine microglial BV-2 cell line. Using CRISPR/Cas9 gene editing, we generated an Acox1-deficient cell line and validated the allelic mutations, which lead to the absence of ACOX1 protein and enzymatic activity...
October 9, 2018: Biochimica et biophysica acta. Molecular and cell biology of lipids
https://www.readbyqxmd.com/read/30311703/shortening-of-apparent-transverse-relaxation-time-of-inorganic-phosphate-as-a-breast-cancer-biomarker
#14
Wybe J M van der Kemp, Tijl A van der Velden, Alexander M Schmitz, Kenneth G Gilhuijs, Peter R Luijten, Dennis W J Klomp, Jannie P Wijnen
Phosphorus MRS offers a non-invasive tool for monitoring cell energy and phospholipid metabolism and can be of additional value in diagnosing cancer and monitoring cancer therapy. In this study, we determined the transverse relaxation times of a number of phosphorous metabolites in a group of breast cancer patients by adiabatic multi-echo spectroscopic imaging at 7 T. The transverse relaxation times of phosphoethanolamine, phosphocholine, inorganic phosphate (Pi ), glycerophosphocholine and glycerophosphatidylcholine were 184 ± 8 ms, 203 ± 17 ms, 87 ± 8 ms, 240 ± 56 ms and 20 ± 10 ms, respectively...
October 12, 2018: NMR in Biomedicine
https://www.readbyqxmd.com/read/30311601/the-chloroplast-2-cysteine-peroxiredoxin-functions-as-thioredoxin-oxidase-in-redox-regulation-of-chloroplast-metabolism
#15
Mohamad-Javad Vaseghi, Kamel Chibani, Wilena Telman, Michael Florian Liebthal, Melanie Gerken, Helena Schnitzer, Sara Mareike Mueller, Karl-Josef Dietz
Thiol-dependent redox regulation controls central processes in plant cells including photosynthesis. Thioredoxins reductively activate e.g. Calvin-Benson cycle enzymes. However the mechanism of oxidative inactivation is unknown despite its importance for efficient regulation. Here, the abundant 2-cysteine peroxiredoxin (2-CysPrx), but not its site-directed variants, mediates rapid inactivation of reductively activated fructose-1,6-bisphosphatase and NADPH-dependent malate dehydrogenase (MDH) in the presence of the proper thioredoxins...
October 12, 2018: ELife
https://www.readbyqxmd.com/read/30311172/bio-guided-purification-and-mass-spectrometry-characterisation-exploring-the-lysozyme-like-protein-from-enterococcus-lactis-q1-an-unusual-marine-bacterial-strain
#16
Olfa Ben Braïek, Slim Smaoui, Yannick Fleury, Stefano Morandi, Khaled Hani, Taoufik Ghrairi
Lactic acid bacteria produce various antibacterial peptides such as bacteriocins that are active against pathogenic and spoilage microorganisms. Very little attention has been paid to the production of lysozyme as an antimicrobial enzyme. The present work represents one of the few studies reporting lysozyme production by enterococci. Indeed, this study was first conducted to evaluate the antimicrobial activity of Enterococcus lactis Q1, an enterocin P-producing strain previously isolated from fresh shrimp (Penaeus vannamei), against multidrug-resistant clinical isolates...
October 12, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/30310249/screening-and-analysis-of-acetyl-cholinesterase-ache-inhibitors-in-the-context-of-alzheimer-s-disease
#17
Mohd Babu Khan, Bhagath Kumar Palaka, Tuleshwori Devi Sapam, Naidu Subbarao, Dinakara Rao Ampasala
Acetyl-cholinesterase enzyme (AChE) is a known target for identifying potential inhibitors against Alzheimer diseases (AD). Therefore, it is of interest to screen AChE with the CNS-BBB database. An AChE enzyme is a member of hydrolase family is activated by acetylcholine (ACh), so, targeting the AChE enzyme with the potential inhibitor may block the binding of the ACh. In this study we carried out virtual screening of drug-like molecules from Chemical Diversity Database particularly CNS-BBB compounds, to identify potential inhibitors using Glide docking program...
2018: Bioinformation
https://www.readbyqxmd.com/read/30309951/confined-acids-catalyze-asymmetric-single-aldolizations-of-acetaldehyde-enolates
#18
Lucas Schreyer, Philip S J Kaib, Vijay N Wakchaure, Carla Obradors, Roberta Properzi, Sunggi Lee, Benjamin List
Reactions that form a product with the same reactive functionality as that of one of the starting compounds frequently end in oligomerization. As a salient example, selective aldol coupling of the smallest, though arguably most useful, enolizable aldehyde, acetaldehyde, with just one partner substrate has proven to be extremely challenging. Here, we report a highly enantioselective Mukaiyama aldol reaction with the simple triethylsilyl (TES) and tert -butyldimethylsilyl (TBS) enolates of acetaldehyde and various aliphatic and aromatic acceptor aldehydes...
October 12, 2018: Science
https://www.readbyqxmd.com/read/30309901/a-ycf2-ftshi-heteromeric-aaa-atpase-complex-is-required-for-chloroplast-protein-import
#19
Shingo Kikuchi, Yukari Asakura, Midori Imai, Yoichi Nakahira, Yoshiko Kotani, Yasuyuki Hashiguchi, Yumi Nakai, Kazuaki Takafuji, Jocelyn Bédard, Yoshino Hirabayashi-Ishioka, Hitoshi Mori, Takashi Shiina, Masato Nakai
Chloroplasts import thousands of nucleus-encoded preproteins synthesized in the cytosol through the TOC and TIC translocons on the outer and inner envelope membranes, respectively. Preprotein translocation across the inner membrane requires ATP; however, the import motor has remained unclear. Here, we report that a 2-MDa heteromeric AAA-ATPase complex associates with the TIC complex and functions as the import motor, directly interacting with various translocating preproteins. This 2-MDa complex consists of a protein encoded by the previously enigmatic chloroplast gene Ycf2 and five related nuclear-encoded FtsH-like proteins, namely FtsHi1, FtsHi2, FtsHi4, FtsHi5, and FtsH12...
October 11, 2018: Plant Cell
https://www.readbyqxmd.com/read/30309407/-role-of-hmgb1-rage-tlrs-nf-%C3%AE%C2%BAb-signaling-pathway-on-bone-mesenchymal-stem-cells-transplantation-therapy-for-lipopolysaccaride-induced-coagulation-disorder-rats
#20
Guanghui Xiu, Wei Xiong, Yunyu Yin, Xianzhong Chen, Ping Liu, Jie Sun, Bin Ling
OBJECTIVE: To determine the effect of bone mesenchymal stem cells (BMSCs) in transplantation therapy for lipopolysaccharide (LPS)-induced coagulation disorder and the underlying mechanism of high mobility group protein B1-receptors for advanced glycation end products/Toll-like receptors-nuclear factor-κB (HMGB1-RAGE/TLRs-NF-κB) signaling pathway. METHODS: BMSCs of female Sprague-Dawley (SD) rats ageing 4-5 weeks old were extracted and cultivated in vitro, and the fourth-passaged BMSCs phenotype was identified by flow cytometry for transplantation in the following experimental study...
September 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
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