keyword
Keywords Antiviral high throughput scre...

Antiviral high throughput screen method

https://read.qxmd.com/read/36700149/a-multivalent-and-thermostable-nanobody-neutralizing-sars-cov-2-omicron-b-1-1-529
#21
JOURNAL ARTICLE
Yuying Lu, Qianlin Li, Huahao Fan, Conghui Liao, Jingsong Zhang, Huan Hu, Huaimin Yi, Yuanli Peng, Jiahai Lu, Zeliang Chen
BACKGROUND: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variants have risen to dominance, which contains far more mutations in the spike protein in comparison to previously reported variants, compromising the efficacy of most existing vaccines or therapeutic monoclonal antibodies. Nanobody screened from high-throughput naïve libraries is a potential candidate for developing preventive and therapeutic antibodies. METHODS: Four nanobodies specific to the SARS-CoV-2 wild-type receptor-binding domain (RBD) were screened from a naïve phage display library...
2023: International Journal of Nanomedicine
https://read.qxmd.com/read/36680291/exploration-of-microbially-derived-natural-compounds-against-monkeypox-virus-as-viral-core-cysteine-proteinase-inhibitors
#22
JOURNAL ARTICLE
Amit Dubey, Maha M Alawi, Thamir A Alandijany, Isra M Alsaady, Sarah A Altwaim, Amaresh Kumar Sahoo, Vivek Dhar Dwivedi, Esam Ibraheem Azhar
Monkeypox virus (MPXV) is a member of the Orthopoxvirus genus and the Poxviridae family, which instigated a rising epidemic called monkeypox disease. Proteinases are majorly engaged in viral propagation by catalyzing the cleavage of precursor polyproteins. Therefore, proteinase is essential for monkeypox and a critical drug target. In this study, high-throughput virtual screening (HTVS) and molecular dynamics simulation were applied to detect the potential natural compounds against the proteinase of the monkeypox virus...
January 16, 2023: Viruses
https://read.qxmd.com/read/36677588/a-non-instrumental-green-analytical-method-based-on-surfactant-assisted-dispersive-liquid-liquid-microextraction-thin-layer-chromatography-smartphone-based-digital-image-colorimetry-sa-dllme-tlc-sdic-for-determining-favipiravir-in-biological-samples
#23
JOURNAL ARTICLE
Bharti Jain, Rajeev Jain, Prashant Kumar Jaiswal, Torki Zughaibi, Tanvi Sharma, Abuzar Kabir, Ritu Singh, Shweta Sharma
Favipiravir (FAV) has become a promising antiviral agent for the treatment of COVID-19. Herein, a green, fast, high-sample-throughput, non-instrumental, and affordable analytical method is proposed based on surfactant-assisted dispersive liquid-liquid microextraction (SA-DLLME) combined with thin-layer chromatography-digital image colourimetry (TLC-DIC) for determining favipiravir in biological and pharmaceutical samples. Triton X-100 and dichloromethane (DCM) were used as the disperser and extraction solvents, respectively...
January 5, 2023: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/36576396/simple-nano-luciferase-based-assay-for-the-rapid-and-high-throughput-detection-of-sars-cov-2-3c-like-protease
#24
JOURNAL ARTICLE
Jingxin Liu, Chenchen Ge, Ling Zha, Ligen Lin, Rongsong Li
In this study, we described an easy-to-perform nano-luciferase (nLuc) sensor for the rapid detection of 3 - chymotrypsin-like protease (3CLpro) encoded by SARS-CoV-2. The technology is based on the cleavage reaction of recombinant-nLuc via 3CLpro. The nLuc-based assay is a general, one-step method and is naturally specific in detection. The stability, sensitivity, detection range, and response time are fully characterized. The application of 3CLpro detection in artificial and human saliva as well as antiviral drug screening demonstrates that the method can quantify 3CLpro with high sensitivity in one step...
December 28, 2022: Analytical Chemistry
https://read.qxmd.com/read/36555652/identification-of-darunavir-derivatives-for-inhibition-of-sars-cov-2-3cl-pro
#25
JOURNAL ARTICLE
Ling Ma, Yongli Xie, Mei Zhu, Dongrong Yi, Jianyuan Zhao, Saisai Guo, Yongxin Zhang, Jing Wang, Quanjie Li, Yucheng Wang, Shan Cen
The effective antiviral agents that treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are urgently needed around the world. The 3C-like protease (3CLpro ) of SARS-CoV-2 plays a pivotal role in virus replication; it also has become an important therapeutic target for the infection of SARS-CoV-2. In this work, we have identified Darunavir derivatives that inhibit the 3CLpro through a high-throughput screening method based on a fluorescence resonance energy transfer (FRET) assay in vitro. We found that the compounds 29# and 50# containing polyphenol and caffeine derivatives as the P2 ligand, respectively, exhibited favorable anti-3CLpro potency with EC50 values of 6...
December 16, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/36382260/novel-computational-pipelines-in-antiviral-structure%C3%A2-based-drug-design-review
#26
REVIEW
Io Diakou, Eleni Papakonstantinou, Louis Papageorgiou, Katerina Pierouli, Konstantina Dragoumani, Demetrios A Spandidos, Flora Bacopoulou, George P Chrousos, Elias Eliopoulos, Dimitrios Vlachakis
Viral infections constitute a fundamental and continuous challenge for the global scientific and medical community, as highlighted by the ongoing COVID-19 pandemic. In combination with prophylactic vaccines, the development of safe and effective antiviral drugs remains a pressing need for the effective management of rare and common pathogenic viruses. The design of potent antivirals can be informed by the study of the three-dimensional structure of viral protein targets. Structure-based design of antivirals in silico provides a solution to the arduous and costly process of conventional drug development pipelines...
December 2022: Biomedical Reports
https://read.qxmd.com/read/36017843/detection-of-ningnanmycin-using-fluorescence-spectroscopy-combined-with-bp-neural-network
#27
JOURNAL ARTICLE
Xiaoyan Wang, Zhezhen Jiang, Rendong Ji, Yue Han, Haiyi Bian, Yudong Yang, Xiaotao Feng, Jiangyu Xu, Mengyuan Wang
BACKGROUND: Ningnanmycin is a new antibiotic pesticide with good bactericidal and antiviral efficacy, which is widely used in the control of fruit and vegetable diseases, and the excessive pesticide residues pose a serious threat to the environment and human health. METHODS: In this study, we used fluorescence spectrometer to scan the three-dimensional spectrum of ningnanmycin samples. We used a BP neural network to complete the regression analysis of content prediction based on the fluorescence spectra...
2023: Combinatorial Chemistry & High Throughput Screening
https://read.qxmd.com/read/35937932/virscan-high-throughput-profiling-of-antiviral-antibody-epitopes
#28
JOURNAL ARTICLE
Ellen L Shrock, Christine L Shrock, Stephen J Elledge
Profiling the specificities of antibodies can reveal a wealth of information about humoral immune responses and the antigens they target. Here, we present a protocol for VirScan, an application of the phage immunoprecipitation sequencing (PhIP-Seq) method for profiling the specificities of human antiviral antibodies. Accompanying this protocol is a video of the experimental procedure. VirScan and, more generally, PhIP-Seq are techniques that enable high-throughput antibody profiling by combining high-throughput DNA oligo synthesis and bacteriophage display with next-generation sequencing...
July 5, 2022: Bio-protocol
https://read.qxmd.com/read/35916773/study-on-aptamer-based-high-throughput-approach-identifies-natural-ingredients-against-rgnnv
#29
JOURNAL ARTICLE
Hongling Wei, Mingzhu Liu, Ke Ke, Shuangyan Xiao, Lin Huang, Qiongyu He, Changping Mo, Hai Pang, Guozhu Xiao, Pengfei Li, Qing Yu
Nervous necrosis virus (NNV) is one of the most destructive pathogens in marine fish aquaculture and is capable of infecting more than 50 fish species worldwide, which resulted in great economic losses. Effective drugs for managing NNV infection are urgently required. Medicinal plants have been known for thousands of years and benefit of medicinal plants against pathogens in aquaculture have emerged. Nowadays, the most commonly used method for detecting virus infection and assessing antiviral drugs efficacy is reverse transcription-quantitative real-time PCR...
November 2022: Journal of Fish Diseases
https://read.qxmd.com/read/35908082/-usnic-acid-and-its-salts-inhibitors-of-sars-cov-2-identified-by-using-in-silico-methods-and-in-vitro-assay
#30
JOURNAL ARTICLE
Eunseok Oh, Weihong Wang, Kyu-Hyung Park, Chanyoon Park, Youbin Cho, JunI Lee, Eunmo Kang, Heonjoong Kang
The pandemic caused by severe acute respiratory Coronavirus-2 (SARS-CoV-2) has been ongoing for over two years, and treatment for COVID-19, other than monoclonal antibodies, is urgently required. Accordingly, we have investigated the inhibitors of SARS-CoV-2 protein targets by high-throughput virtual screening using a marine natural products database. Considering the calculated molecular properties and availability of the compounds, (+)-usnic acid was selected as a suitable hit. In the in vitro antiviral assay of (+)-usnic acid by the immunofluorescence method, IC50 was 7...
July 30, 2022: Scientific Reports
https://read.qxmd.com/read/35866161/rt-qpcr-as-a-screening-platform-for-mutational-and-small-molecule-impacts-on-structural-stability-of-rna-tertiary-structures
#31
JOURNAL ARTICLE
Martina Zafferani, Dhanasheel Muralidharan, Nadeska I Montalvan, Amanda E Hargrove
The exponential increase in the discovery and characterization of RNA tertiary structures has highlighted their active role in a variety of human diseases, yet often their interactome and specific function remain unknown. Small molecules offer opportunities to both decode these cellular roles and develop therapeutics, however there are few examples of small molecules that target biologically relevant RNA tertiary structures. While RNA triple helices are a particularly attractive target, discovery of triple helix modulators has been hindered by the lack of correlation between small molecule affinity and effect on structural modulation, thereby limiting the utility of affinity-based screening as a primary filtering method...
July 6, 2022: RSC chemical biology
https://read.qxmd.com/read/35822715/kinetic-characterization-of-sars-cov-2-nsp13-atpase-activity-and-discovery-of-small-molecule-inhibitors
#32
JOURNAL ARTICLE
Aliakbar Khalili Yazdi, Paknoosh Pakarian, Sumera Perveen, Taraneh Hajian, Vijayaratnam Santhakumar, Albina Bolotokova, Fengling Li, Masoud Vedadi
SARS-CoV-2 non-structural protein 13 (nsp13) is a highly conserved helicase and RNA 5'-triphosphatase. It uses the energy derived from the hydrolysis of nucleoside triphosphates for directional movement along the nucleic acids and promotes the unwinding of double-stranded nucleic acids. Nsp13 is essential for replication and propagation of all human and non-human coronaviruses. Combined with its defined nucleotide binding site and druggability, nsp13 is one of the most promising candidates for the development of pan-coronavirus therapeutics...
August 12, 2022: ACS Infectious Diseases
https://read.qxmd.com/read/35821477/generation-characterization-and-applications-of-influenza-a-reporter-viruses
#33
JOURNAL ARTICLE
Kevin Chiem, Aitor Nogales, Luis Martinez-Sobrido
Secondary experimental procedures such as immunostaining have been utilized to study wild-type influenza A viruses (IAV) but are inadequate to rapidly determine the virus in infected cells or for the high-throughput screening (HTS) of antivirals or neutralizing antibodies. Reverse genetics approaches have allowed the generation of recombinant IAV expressing bioluminescent (BL) reporters or fluorescent proteins (FPs). These approaches can easily track viral infections in cultured cells and in validated animal models of infection using in vivo imaging systems (IVIS)...
2022: Methods in Molecular Biology
https://read.qxmd.com/read/35761502/review-on-in-silico-methods-high-throughput-screening-techniques-and-cell-culture-based-in-vitro-assays-for-sars-cov-2
#34
JOURNAL ARTICLE
Yuksel Cetin, Seyma Aydinlik, Aysen Gungor, Tugce Kan, Timucin Avsar, Serdar Durdagi
The COVID-19 outbreak caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) continues to have high incidence and mortality rate globally. To meet the increasingly growing demand for new therapeutic drugs and vaccines, researchers are developing different diagnostic techniques focused on screening new drugs in clinical use, developing an antibody targeting a SARS-CoV-2 receptor, or interrupting infection/replication mechanisms of SARS-CoV-2. Although many prestigious research publications are addressing this subject, there is no open access platform where all experimental techniques for COVID-19 research can be seen as a whole...
June 27, 2022: Current Medicinal Chemistry
https://read.qxmd.com/read/35746713/aptamer-based-high-throughput-screening-model-for-efficient-selection-and-evaluation-of-natural-ingredients-against-sgiv-infection
#35
JOURNAL ARTICLE
Hongling Wei, Zhongbao Guo, Yu Long, Mingzhu Liu, Jun Xiao, Lin Huang, Qing Yu, Pengfei Li
Singapore grouper iridovirus (SGIV) causes high economic losses in mariculture. Effective drugs for managing SGIV infection are urgently required. Medicinal plant resources are rich in China. Medicinal plants have a long history and significant curative effects in the treatment of many diseases. Reverse-transcription quantitative real-time PCR is the most commonly used method for detecting virus infection and assessing antiviral efficacy with high accuracy. However, their applications are limited due to high reagent costs and complex time-consuming operations...
June 8, 2022: Viruses
https://read.qxmd.com/read/35640847/a-novel-high-throughput-single-b-cell-cloning-platform-for-isolation-and-characterization-of-high-affinity-and-potent-sars-cov-2-neutralizing-antibodies
#36
JOURNAL ARTICLE
Paritosh Prashar, Sonali Swain, Nisha Adhikari, Punit Aryan, Anupama Singh, Mohit Kwatra, Prabhakar B
Monoclonal antibodies (mAbs) that are specific to SARS-CoV-2 can be useful in diagnosing, preventing, and treating the coronavirus (COVID-19) illness. Strategies for the high-throughput and rapid isolation of these potent neutralizing antibodies are critical toward the development of therapeutically targeting COVID-19 as well as other infectious diseases. In the present study, a single B-cell cloning method was used to screen the Wuhan-Hu-1 strain of SARS-CoV-2 receptor-binding domain (RBD) specific, high affinity, and neutralizing mAbs from patients' blood samples...
May 28, 2022: Antiviral Research
https://read.qxmd.com/read/35513270/screening-and-identification-of-htnv-pv-entry-inhibitors-with-high-throughput-pseudovirus-based-chemiluminescence
#37
JOURNAL ARTICLE
Xiaojing Wen, Li Zhang, Qiang Liu, Xinyue Xiao, Weijin Huang, Youchun Wang
Hantaviruses, such as Hantaan virus (HTNV) and Seoul virus, are the causative agents of Hantavirus cardiopulmonary syndrome (HCPS) and hemorrhagic fever with renal syndrome (HFRS), and are important zoonotic pathogens. China has the highest incidence of HFRS, which is mainly caused by HTNV and Seoul virus. No approved antiviral drugs are available for these hantaviral diseases. Here, a chemiluminescence-based high-throughput-screening (HTS) assay was developed and used to screen HTNV pseudovirus (HTNVpv ) inhibitors in a library of 1813 approved drugs and 556 small-molecule compounds from traditional Chinese medicine sources...
May 2, 2022: Virologica Sinica
https://read.qxmd.com/read/35476790/development-of-a-high-throughput-method-to-screen-novel-antiviral-materials
#38
JOURNAL ARTICLE
Makoto Nakakido, Naoki Tanaka, Ayako Shimojo, Nobuhiro Miyamae, Kouhei Tsumoto
Respiratory infectious diseases pose a serious threat worldwide, and novel antiviral materials are highly demanded. Photocatalytic nanoparticles have been developed to inhibit indirect transmission of pathogens by acting as surface coating materials. During development of such antiviral materials, researchers use bacteriophages as model viruses due to their safety and experimental efficiency. Screening methods are used to identify potential antiviral materials, and better screening technologies will accelerate the discovery of antiviral treatments...
2022: PloS One
https://read.qxmd.com/read/35379146/strategies-in-medicinal-chemistry-to-discovery-new-hits-compounds-against-ebola-virus-challenges-and-perspectives-in-drug-discovery
#39
JOURNAL ARTICLE
Igor José Dos Santos Nascimento, Paulo Fernando da Silva Santos-Júnior, João Xavier de Araújo-Júnior, Edeildo Ferreira da Silva-Júnior
Ebola virus (EBOV) is an infectious disease that mainly affects the cardiovascular system. It belongs to the Filoviridae family, consisting of filamentous envelopes, non-segmented negative RNA genome. EBOV was initially identified in Sudan and Zaire (now named the Democratic Republic of Congo), around 1967. It is transmitted mainly by contact with secretions (blood, sweat, saliva, and tears) from infected wild animals, such as non-human primates and bats. It has gained more prominence in recent years, due to the recent EBOV outbreaks that occurred from 2013 to 2016, resulting in approximately 28,000 infected individuals, with a mortality rate of 40-70%, affecting mainly Liberia, Guinea, and Sierra Leone...
April 4, 2022: Mini Reviews in Medicinal Chemistry
https://read.qxmd.com/read/35075100/virtual-screening-and-molecular-docking-to-study-the-mechanism-of-chinese-medicines-in-the-treatment-of-coronavirus-infection
#40
JOURNAL ARTICLE
Fan Ping, Yanxia Wang, Xia Shen, Conge Tan, Lin Zhu, Wenwen Xing, Jun Xu
BACKGROUND Heat-clearing and detoxifying herbs (HDHs) play an important role in the prevention and treatment of coronavirus infection. However, their mechanism of action needs further study. This study aimed to explore the anti-coronavirus basis and mechanism of HDHs. MATERIAL AND METHODS Database mining was performed on 7 HDHs. Core ingredients and targets were screened according to ADME rules combined with Neighborhood, Co-occurrence, Co-expression, and other algorithms. GO enrichment and KEGG pathway analyses were performed using the R language...
January 25, 2022: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
keyword
keyword
158439
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.