keyword
https://read.qxmd.com/read/38534967/amino-acid-metabolism-in-leukocytes-showing-in-vitro-igg-memory-from-sars-cov2-infected-patients
#1
REVIEW
Giuseppina Fanelli, Veronica Lelli, Sara Rinalducci, Anna Maria Timperio
The immune response to infectious diseases is directly influenced by metabolic activities. COVID-19 is a disease that affects the entire body and can significantly impact cellular metabolism. Recent studies have focused their analysis on the potential connections between post-infection stages of SARS-CoV2 and different metabolic pathways. The spike S1 antigen was found to have in vitro IgG antibody memory for PBMCs when obtaining PBMC cultures 60-90 days post infection, and a significant increase in S-adenosyl homocysteine, sarcosine, and arginine was detected by mass spectrometric analysis...
February 23, 2024: Diseases (Basel)
https://read.qxmd.com/read/38517663/co-solutes-induced-changes-in-the-properties-of-polymeric-solution-and-water-dynamics
#2
JOURNAL ARTICLE
Sanyukta Bhattacharjee, Souvik Pandit, Debabrata Seth
In this paper we are addressing the co-solute-induced changes in the properties of an aqueous solution of a block copolymer. Due to the preferential interaction of different co-solute with different regions of the block copolymer, the changes were observed in both the physical properties and water dynamics. The modulation of both the physical properties and water dynamics was monitored using different spectroscopic techniques. Different co-solutes affect micellar properties of copolymer to a different extent signifying their interactions with different regions within the copolymer...
March 22, 2024: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://read.qxmd.com/read/38501746/choline-degradation-in-paracoccus-denitrificans-identification-of-sources-of-formaldehyde
#3
JOURNAL ARTICLE
Trusha Parekh, Marcus Tsai, Stephen Spiro
Paracoccus denitrificans is a facultative methylotroph that can grow on methanol and methylamine as sole sources of carbon and energy. Both are oxidized to formaldehyde and then to formate, so growth on C1 substrates induces the expression of genes encoding enzymes required for the oxidation of formaldehyde and formate. This induction involves a histidine kinase response regulator pair (FlhSR) that is likely triggered by formaldehyde. Catabolism of some complex organic substrates (e.g., choline and L-proline betaine) also generates formaldehyde...
March 19, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38486406/amino-acids-and-glycine-derivatives-differently-affect-refolding-of-mesophilic-and-thermophilic-like-%C3%AE-amylases-implications-in-protein-refolding-and-aggregation
#4
JOURNAL ARTICLE
Aziz Ahmad, Prachi Joshi, Rajesh Mishra
α-amylases are industrially important enzymes which are used in different starch-based industries. They are adapted to different environmental conditions like extremes of temperature, pH and salinity. Herein, α-amylases from Bacillus amyloliquifaciens (BAA) and Bacillus licheniformis (BLA), representing mesophilic and thermophilic-like proteins, respectively, have been used to investigate the effect of naturally occurring osmolytes like arginine, proline, glycine and its methyl derivatives, sarcosine and betaine on their refolding...
March 14, 2024: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/38483649/targeted-metabolomic-analysis-of-serum-amino-acids-in-heart-failure-patients
#5
JOURNAL ARTICLE
Chunjing Yang, Zhengyuan Shi, Li Bao, Xiqiao Xv, Dechun Jiang, Longtai You
Heart failure (HF) has been recognized as a global epidemic with high rates of morbidity, hospitalization, and mortality. The role of amino acids, which provide the body with energy, in the development of HF is still unclear. The aim of this study was to explore changes in serum amino acids in patients with HF and identify potential biomarkers. First, the serum amino acid metabolism profiles of 44 patients with HF and 30 healthy controls (Con) were quantitatively measured. Then, candidate markers were identified through the utilization of T test, multivariate statistical analysis, and receiver operating characteristic (ROC) curve analysis...
March 14, 2024: Amino Acids
https://read.qxmd.com/read/38467100/one-step-construction-of-multiplexed-enzymatic-biosensors-using-light-addressable-electrochemistry-on-a-single-silicon-photoelectrode
#6
JOURNAL ARTICLE
Qiaoyu Yang, Jiaming Liao, Luyao Feng, Sen Wang, Zhibin Zhao, Jian Wang, Yazhong Bu, Jian Zhuang, De-Wen Zhang
The multiplexed detection of metabolites in parallel within a single biosensor plate is sufficiently valuable but also challenging. Herein, we combine the inherent light addressability of silicon with the high selectivity of enzymes, for the construction of multiplexed photoelectrochemical enzymatic biosensors. To conduct a stable electrochemistry and reagentless biosensing on silicon, a new strategy involving the immobilization of both redox mediators and enzymes using an amide bond-based hydrogel membrane was proposed...
March 6, 2024: Biosensors & Bioelectronics
https://read.qxmd.com/read/38430121/exploring-the-effects-of-cyclosporin-a-to-isocyclosporin-a-rearrangement-on-ion-mobility-separation
#7
JOURNAL ARTICLE
Hynek Mácha, Jakub Zápal, Marek Kuzma, Dominika Luptáková, Karel Lemr, Vladimír Havlíček
Cyclosporin A (CycA) is a peptide secondary metabolite derived from fungi that plays a crucial role in transplantation surgery. Cyclic traveling wave ion mobility mass spectrometry (IM-MS) revealed an N → O peptidyl shift in singly protonated CycA to isocyclosporin A (isoA), whereas no such isomerization was observed for doubly protonated and sodiated molecules. CycA and isoA were able to be separated by considering doubly protonated precursors using a specific ion fragment. In parallel, sodium ion stabilization facilitated the simultaneous separation and quantitation of singly charged cyclosporin isomers with the limit of detection and coefficient of determination of 1...
March 2, 2024: Analytical Chemistry
https://read.qxmd.com/read/38425413/investigating-d-amino-acid-oxidase-expression-and-interaction-network-analyses-in-pathways-associated-with-cellular-stress-implications-in-the-biology-of-aging
#8
JOURNAL ARTICLE
V Kalidasan, Darshinie Suresh, Nurulisa Zulkifle, Yap Siew Hwei, Leong Kok Hoong, Reena Rajasuriar, Kumitaa Theva Das
D-amino acid oxidase (DAO) is a flavoenzyme that metabolizes D-amino acids by oxidative deamination, producing hydrogen peroxide (H2 O2 ) as a by-product. The generation of intracellular H2 O2 may alter the redox-homeostasis mechanism of cells and increase the oxidative stress levels in tissues, associated with the pathogenesis of age-related diseases and organ decline. This study investigates the effect of DAO knockdown using clustered regularly interspaced short palindromic repeats (CRISPR) through an in silico approach on its protein-protein interactions (PPIs) and their potential roles in the process of aging...
2024: Bioinformatics and Biology Insights
https://read.qxmd.com/read/38420856/effects-of-mixed-extract-from-two-tropical-plants-on-gut-microbiome-and-metabolome-in-piglets
#9
JOURNAL ARTICLE
Luli Zhou, Guanyu Hou, Shengmin Liu, Hanlin Zhou, Yuxiu Ye, Renlong Lv, Khaled Abouelezz, Dingfa Wang
In this study, we performed a quantitative analysis of 12 compounds derived from Piper sarmentosum extract (PSE) and guava leaf extract (GE). In addition, we investigated the effects of mixed extract (ME) of PSE and GE (1:1) on piglets' gut microbiome and metabolome. A total of 200 piglets (Duroc × Landrace × Large Yorkshire, 21-day-old) were randomly assigned into two groups with five replicates of 20 piglets/pen having the same initial body weight. Piglets were fed a basal diet supplemented with ME at 0 (T0) or 200 mg/kg (T1) for 3 weeks...
February 29, 2024: Journal of Animal Physiology and Animal Nutrition
https://read.qxmd.com/read/38387153/targeted-arginine-metabolomics-combined-with-metagenomics-revealed-the-potential-mechanism-of-pueraria-lobata-extract-in-treating-myocardial-infarction
#10
JOURNAL ARTICLE
Bojiao Yi, Yurou Zhao, Han Yan, Zeyu Li, Pin Zhang, Zhengyu Fang, Yuping Zhao, Hongjun Yang, Na Guo
The extraction methods for traditional Chinese medicine (TCM) may have varying therapeutic effects on diseases. Currently, Pueraria lobata (PL) is mostly extracted with ethanol, but decoction, as a TCM extraction method, is not widely adopted. In this study, we present a strategy that integrates targeted metabolomics, 16 s rDNA sequencing technology and metagenomics for exploring the potential mechanism of the water extract of PL (PLE) in treating myocardial infarction (MI). Using advanced analytical techniques like ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), we comprehensively characterized PLE's chemical composition...
February 10, 2024: Journal of Chromatography. A
https://read.qxmd.com/read/38359327/precision-synthesis-of-polysarcosine-via-controlled-ring-opening-polymerization-of-n-carboxyanhydride-fast-kinetics-ultrahigh-molecular-weight-and-mechanistic-insights
#11
JOURNAL ARTICLE
Shuo Wang, Ming-Yuan Lu, Si-Kang Wan, Chun-Yan Lyu, Zi-You Tian, Kai Liu, Hua Lu
The rapid and controlled synthesis of high-molecular-weight (HMW) polysarcosine (pSar), a potential polyethylene glycol (PEG) alternative, via the ring-opening polymerization (ROP) of N -carboxyanhydride (NCA) is rare and challenging. Here, we report the well-controlled ROP of sarcosine NCA (Sar-NCA) that is catalyzed by various carboxylic acids, which accelerate the polymerization rate up to 50 times, and enables the robust synthesis of pSar with an unprecedented ultrahigh molecular weight (UHMW) up to 586 kDa (DP ∼ 8200) and exceptionally narrow dispersity ( D̵ ) below 1...
February 15, 2024: Journal of the American Chemical Society
https://read.qxmd.com/read/38355897/construction-of-double-enzyme-complexes-with-dna-framework-nanorulers-for-improving-enzyme-cascade-catalytic-efficiency
#12
JOURNAL ARTICLE
Nan Cao, Shaopeng Wang, Fan Li, Xiuhai Mao, Xiaolei Zuo, Yueyue Zhang, Min Li
Efficient biocatalytic cascade reactions play a crucial role in guiding intricate, specific and selective intracellular transformation processes. However, the catalytic activity of the enzyme cascade reaction in bulk solution was greatly impacted by the spatial morphology and inter-enzyme distance. The programmability and addressability nature of framework nucleic acid (FNA) allows to be used as scaffold for immobilization and to direct the spatial arrangement of enzyme cascade molecules. Here, we used tetrahedral DNA framework (TDF) as nanorulers to assemble two enzymes for constructing a double-enzyme complex, which significantly enhance the catalytic efficiency of sarcosine oxidase (SOx)/horseradish peroxidase (HRP) cascade system...
February 14, 2024: ChemPlusChem
https://read.qxmd.com/read/38343179/facile-preparation-of-polysarcosine-tethered-inorganic-nanospheres-by-using-unimolecular-polypept-o-ides-as-template
#13
JOURNAL ARTICLE
Xikuang Yao, Linhui Hao, Ting Wang, Fei Xiong, Qian Shen, Wei Huang
Polymer-inorganic nanocomposites that integrate the advantages of both polymers and inorganic nanoparticles (NPs) are broadly exploited for versatile applications. Especially, emerging polymer-templated preparation of inorganic NPs has drawn extensive attention, which is ascribed to simplified synthesis and feasible tunability. However, how to precisely fabricate biocompatible polymer-inorganic NPs remains unsolved. In this article, by mild ring opening polymerization (ROP) of β-benzyl L-aspartate N-carboxyanhydrides (BLA-NCAs) and sarcosine N-carboxyanhydrides (Sar-NCAs) and subsequent debenzylation, a series of poly(amino acid)-based unimolecular micelles (PAMAM-g-(PLA-b-PSar)) are facilely synthesized...
February 11, 2024: Small Methods
https://read.qxmd.com/read/38320697/nitrogen-fertilizer-addition-to-an-agricultural-soil-enhances-biogenic-non-extractable-residue-formation-from-2-13-c-15-n-glyphosate
#14
JOURNAL ARTICLE
Sohaib Aslam, Karolina M Nowak
Glyphosate and nitrogen (N) or (P) phosphorus fertilizers are often applied in combination to agricultural fields. The additional P or N supply to microorganisms might drive glyphosate degradation towards the sarcosine/glycine or the aminomethylphosphonic acid (AMPA), and consequently determine the speciation of non-extractable residues (NERs): harmless biogenic NERs (bioNERs) or potentially hazardous xenobiotic NERs (xenoNERs). We therefore investigated the effect of P or N-fertilizers on microbial degradation of glyphosate and bioNER formation in an agricultural soil...
February 4, 2024: Science of the Total Environment
https://read.qxmd.com/read/38315159/tunable-approach-to-diverse-phenethylamines-via-reduction-of-5-aryloxazolidines-with-triethylsilane
#15
JOURNAL ARTICLE
Anastasia A Smorodina, Evgeny M Buev, Vladimir S Moshkin, Vyacheslav Y Sosnovskikh
A rapid pathway for the synthesis of various β-phenethylamines from aromatic aldehydes has been developed. Initially, a wide range of 5-aryloxazolidines was prepared via the [3 + 2] cycloaddition reaction of N -methylazomethine ylide derived from sarcosine and paraformaldehyde. The subsequent reduction of 5-aryloxazolidines with triethylsilane in trifluoroacetic acid yields three types of products: N , N -dimethylphenylethanolamines, N , N -dimethylphenethylamines, and tetrahydroisoquinolines, depending on the substituents in the aromatic ring and reaction conditions...
February 5, 2024: Journal of Organic Chemistry
https://read.qxmd.com/read/38311611/valorization-potential-of-egyptian-mango-kernel-waste-product-as-analyzed-via-gc-ms-metabolites-profiling-from-different-cultivars-and-geographical-origins
#16
JOURNAL ARTICLE
Rehan M El-Shabasy, Tarek F Eissa, Yossef Emam, Ahmed Zayed, Nesrin Fayek, Mohamed A Farag
Increasing attention has been given to mango (Mangifera indica) fruits owing to their characteristic taste, and rich nutritional value. Mango kernels are typically discarded as a major waste product in mango industry, though of potential economic value. The present study aims to outline the first comparison of different mango kernel cvs. originated from different localities alongside Egypt, e.g., Sharqia, Suez, Ismailia, and Giza. Gas chromatography-mass spectroscopy (GC-MS) post silylation analysis revealed that sugars were the major class being detected at 3...
February 5, 2024: Scientific Reports
https://read.qxmd.com/read/38304426/metabolomic-and-immune-alterations-in-long-covid-patients-with-chronic-fatigue-syndrome
#17
JOURNAL ARTICLE
Suguru Saito, Shima Shahbaz, Xian Luo, Mohammed Osman, Desiree Redmond, Jan Willem Cohen Tervaert, Liang Li, Shokrollah Elahi
INTRODUCTION: A group of SARS-CoV-2 infected individuals present lingering symptoms, defined as long COVID (LC), that may last months or years post the onset of acute disease. A portion of LC patients have symptoms similar to myalgic encephalomyelitis or chronic fatigue syndrome (ME/CFS), which results in a substantial reduction in their quality of life. A better understanding of the pathophysiology of LC, in particular, ME/CFS is urgently needed. METHODS: We identified and studied metabolites and soluble biomarkers in plasma from LC individuals mainly exhibiting ME/CFS compared to age-sex-matched recovered individuals (R) without LC, acute COVID-19 patients (A), and to SARS-CoV-2 unexposed healthy individuals (HC)...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38292682/multi-omics-revealed-the-formation-mechanism-of-characteristic-volatiles-in-tibetan-yak-cheese-induced-by-different-altitudes
#18
JOURNAL ARTICLE
Bei Xue, Guo Li, Xujia Xun, Qun Huang, Shaokang Wang
The variation in volatiles, bacteria and metabolites of Tibetan yak cheese (TYC) from different altitudes were characterized with multi-omics to reveal the formation mechanism of characteristic volatile compounds (C-VOCs) in TYC induced by altitudes. 22C-VOCs (odor activity value, OAV > 1) were identified in TYCs, and hexanal, dodecanol, 2,3-butanediol, butyl isobutyate, etc., C-VOCs were confirmed induced by altitude. Lactobacillus , Kocuria , etc., bacteria and benzyl thiocyanate, trehalose, sarcosine, etc...
March 30, 2024: Food chemistry: X
https://read.qxmd.com/read/38283563/exploring-the-mechanism-of-immediate-analgesia-induced-by-tuina-intervention-on-minor-chronic-constriction-injury-in-rats-using-lc-ms
#19
JOURNAL ARTICLE
Sachula, Zhenjie Yang, Tianyuan Yu, Jinping Chen, Runlong Zhang, Yingqi Zhang, Jiayue Liu, Hanyu Zhang, Jiawei Sun
PURPOSE: This study aimed to investigate changes in metabolomic expression in the spinal dorsal horn (SDH) and thalamus during a Tuina session, aiming to elucidate the mechanism of immediate analgesia. METHODS: The rats were randomly divided into three groups: the Sham group, the Model group, and the Tuina group. A minor chronic constriction injury (minor CCI) model was established in both the Model group and the Tuina group. The therapeutic effect of Tuina was determined using the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) tests...
2024: Journal of Pain Research
https://read.qxmd.com/read/38280784/aptasensor-based-on-high-surface-area-covalent-organic-framework-for-simple-and-ultrasensitive-detection-of-sarcosine-in-the-diagnosis-of-prostate-cancer
#20
JOURNAL ARTICLE
Fatemeh Hamdi, Mahmoud Roushani, Mina Nasibipour, S Jafar Hoseini
In this study, an electrochemical aptasensor was developed for the specific detection of sarcosine using a covalent organic framework (COF). The imine-based two-dimensional COF was synthesized through a solvothermal process using terephthaldehyde and melamine. This resulted in the formation of a structure that is highly porous and has a unique surface area of 908 m2 /g. The produced biosensor demonstrated a significant linear relationship between charge transfer resistance (Rct) and various concentrations of sarcosine in blood serum samples...
February 22, 2024: Analytica Chimica Acta
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