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https://www.readbyqxmd.com/read/28241496/the-role-of-sarcosine-uracil-and-kynurenic-acid-metabolism-in-urine-for-diagnosis-and-progression-monitoring-of-prostate-cancer
#1
Georgios Gkotsos, Christina Virgiliou, Ioanna Lagoudaki, Chrysanthi Sardeli, Nikolaos Raikos, Georgios Theodoridis, Georgios Dimitriadis
The aim of this pilot study is to evaluate sarcosine, uracil, and kynurenic acid in urine as potential biomarkers in prostate cancer detection and progression monitoring. Sarcosine, uracil, and kynurenic acid were measured in urine samples of 32 prostate cancer patients prior to radical prostatectomy, 101 patients with increased prostate-specific antigen prior to ultrasonographically-guided prostatic biopsy collected before and after prostatic massage, and 15 healthy volunteers (controls). The results were related to histopathologic data, Gleason score, and PSA (Prostate Specific Antigen)...
February 23, 2017: Metabolites
https://www.readbyqxmd.com/read/28236690/binding-interaction-of-sodium-n-dodecanoyl-sarcosinate-with-hemoglobin-and-myoglobin-physicochemical-and-spectroscopic-studies-with-molecular-docking-analysis
#2
Suparna Rudra, Somnath Dasmandal, Ambikesh Mahapatra
The interaction of an amino acid surfactant, sodium-N-dodecanoyl sarcosinate (SDDS), with two heme proteins, hemoglobin (Hb) and myoglobin (Mb), has been studied employing various physicochemical and spectroscopic techniques like tensiometry, UV-Vis spectroscopy, steady-state fluorometry, time-resolved fluorometry, circular dichroism (CD) spectroscopy, calorimetry and stopped flow kinetics at physiological pH of 7.2 and 298K. Tensiometric and fluorometric analysis suggest that the interaction between SDDS and protein starts with the monomer form of the surfactant which produces small induced micelles...
February 16, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28207877/selective-inhibition-of-aggregation-fibrillation-of-bovine-serum-albumin-by-osmolytes-mechanistic-and-energetics-insights
#3
Moumita Dasgupta, Nand Kishore
Bovine serum albumin (BSA) is an important transport protein of the blood and its aggregation/fibrillation would adversely affect its transport ability leading to metabolic disorder. Therefore, understanding the mechanism of fibrillation/aggregation of BSA and design of suitable inhibitor molecules for stabilizing its native conformation, are of utmost importance. The qualitative and quantitative aspects of the effect of osmolytes (proline, hydroxyproline, glycine betaine, sarcosine and sorbitol) on heat induced aggregation/fibrillation of BSA at physiological pH (pH 7...
2017: PloS One
https://www.readbyqxmd.com/read/28194962/high-speed-quantitative-uplc-ms-analysis-of-multiple-amines-in-human-plasma-and-serum-via-precolumn-derivatization-with-6-aminoquinolyl-n-hydroxysuccinimidyl-carbamate-application-to-acetaminophen-induced-liver-failure
#4
Nicola Gray, Rabiya Zia, Adam King, Vishal C Patel, Julia Wendon, Mark J W McPhail, Muireann Coen, Robert S Plumb, Ian D Wilson, Jeremy K Nicholson
A targeted reversed-phase gradient UPLC-MS/MS assay has been developed for the quantification /monitoring of 66 amino acids and amino-containing compounds in human plasma and serum using precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AccQTag Ultra). Derivatization of the target amines required minimal sample preparation and resulted in analytes with excellent chromatographic and mass spectrometric detection properties. The resulting method, which requires only 10 μL of sample, provides the reproducible and robust separation of 66 analytes in 7...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28188523/full-membrane-protein-coverage-digestion-and-quantitative-bottom-up-mass-spectrometry-proteomics
#5
Joseph Capri, Julian P Whitelegge
A true and accurate bottom-up global proteomic measurement will only be achieved when all proteins in a sample can be digested efficiently and at least some peptides recovered on which to base an estimate of abundance. Integral membrane proteins make up around one-third of the proteome and require specialized protocols if they are to be successfully solubilized for efficient digestion by the enzymes used in bottom-up proteomics. The protocol described relies upon solubilization using the detergents sodium deoxycholate and lauryl sarcosine with heating to 95 °C...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28185807/specific-circulating-phospholipids-acylcarnitines-amino-acids-and-biogenic-amines-are-aerobic-exercise-markers
#6
Thomas K Felder, Susanne Ring-Dimitriou, Simon Auer, Selma M Soyal, Ludmilla Kedenko, Mark Rinnerthaler, Janne Cadamuro, Elisabeth Haschke-Becher, Elmar Aigner, Bernhard Paulweber, Wolfgang Patsch
OBJECTIVES: Regular aerobic exercise provides beneficial effects on human health and reduces all-cause mortality. Aerobic exercise has profound metabolic effects, and specific metabolites may reflect physiological changes. We aimed to identify endogenous metabolites that distinguish the trained from the untrained state to increase the spectrum of analytes amenable for hypothesis testing and to expand understanding of putative beneficial pathways. DESIGN: Cross sectional laboratory repeated measures study...
January 24, 2017: Journal of Science and Medicine in Sport
https://www.readbyqxmd.com/read/28174298/the-startle-disease-mutation-e103k-impairs-activation-of-human-homomeric-%C3%AE-1-glycine-receptors-by-disrupting-an-intersubunit-salt-bridge-across-the-agonist-binding-site
#7
Fatemah Safar, Elliot Hurdiss, Marios Erotocritou, Timo Greiner, Remigijus Lape, Mark W Irvine, Guangyu Fang, David Jane, Rilei Yu, Marc A Dämgen, Philip C Biggin, Lucia G Sivilotti
Glycine receptors (GlyR) belong to the pentameric ligand gated ion channel (pLGIC) superfamily and mediate fast inhibitory transmission in the vertebrate CNS. Disruption of glycinergic transmission by inherited mutations produces startle disease in man. Many startle mutations are in GlyRs and provide useful clues to the function of the channel domains. E103K is one of few startle mutations found in the extracellular agonist binding site of the channel, in loop A of the principal side of the subunit interface...
February 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28159969/targeted-metabolomics-reveals-early-dominant-optic-atrophy-signature-in-optic-nerves-of-opa1delttag-mice
#8
Juan Manuel Chao de la Barca, Gilles Simard, Emmanuelle Sarzi, Tanguy Chaumette, Guillaume Rousseau, Stéphanie Chupin, Cédric Gadras, Lydie Tessier, Marc Ferré, Arnaud Chevrollier, Valérie Desquiret-Dumas, Naïg Gueguen, Stéphanie Leruez, Christophe Verny, Dan Miléa, Dominique Bonneau, Patrizia Amati-Bonneau, Vincent Procaccio, Christian Hamel, Guy Lenaers, Pascal Reynier, Delphine Prunier-Mirebeau
Purpose: Dominant optic atrophy (MIM No. 165500) is a blinding condition related to mutations in OPA1, a gene encoding a large GTPase involved in mitochondrial inner membrane dynamics. Although several mouse models mimicking the disease have been developed, the pathophysiological mechanisms responsible for retinal ganglion cell degeneration remain poorly understood. Methods: Using a targeted metabolomic approach, we measured the concentrations of 188 metabolites in nine tissues, that is, brain, three types of skeletal muscle, heart, liver, retina, optic nerve, and plasma in symptomatic 11-month-old Opa1delTTAG/+ mice...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28123936/metabolomic-profiling-identifies-potential-pathways-involved-in-the-interaction-of-iron-homeostasis-with-glucose-metabolism
#9
Lars Stechemesser, Sebastian K Eder, Andrej Wagner, Wolfgang Patsch, Alexandra Feldman, Michael Strasser, Simon Auer, David Niederseer, Ursula Huber-Schönauer, Bernhard Paulweber, Stephan Zandanell, Sandra Ruhaltinger, Daniel Weghuber, Elisabeth Haschke-Becher, Christoph Grabmer, Eva Rohde, Christian Datz, Thomas K Felder, Elmar Aigner
OBJECTIVE: Elevated serum ferritin has been linked to type 2 diabetes (T2D) and adverse health outcomes in subjects with the Metabolic Syndrome (MetS). As the mechanisms underlying the negative impact of excess iron have so far remained elusive, we aimed to identify potential links between iron homeostasis and metabolic pathways. METHODS: In a cross-sectional study, data were obtained from 163 patients, allocated to one of three groups: (1) lean, healthy controls (n = 53), (2) MetS without hyperferritinemia (n = 54) and (3) MetS with hyperferritinemia (n = 56)...
January 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28101932/amino-acid-profiling-of-zinc-resistant-prostate-cancer-cell-lines-associations-with-cancer-progression
#10
Monika Kratochvilova, Martina Raudenska, Zbynek Heger, Lukas Richtera, Natalia Cernei, Vojtech Adam, Petr Babula, Marie Novakova, Michal Masarik, Jaromir Gumulec
BACKGROUND: Failure in intracellular zinc accumulation is a key process in prostate carcinogenesis. Nevertheless, epidemiological studies of zinc administration have provided contradicting results. In order to examine the impact of the artificial intracellular increase of zinc(II) ions on prostate cancer metabolism, PNT1A, 22Rv1, and PC-3 prostatic cell lines-depicting different stages of cancer progression-and their zinc-resistant counterparts were used. To determine "benign" and "malignant" metabolic profiles, amino acid patterns, gene expression, and antioxidant capacity of these cell lines were assessed...
January 19, 2017: Prostate
https://www.readbyqxmd.com/read/28079404/free-amino-acids-in-fibromyalgia-syndrome-relationship-with-clinical-picture
#11
Valeria Ruggiero, Massimiliano Mura, Enrico Cacace, Benedetta Era, Marcella Peri, Giuseppina Sanna, Antonella Fais
The objectives of our study were to evaluate free amino acid (FAA) concentrations in the serum of patients affected by fibromyalgia syndrome (FMS) and to determine the relationships between FAA levels and FMS clinical parameters. Thus, serum amino acid concentrations were quantified (HPLC analysis) in 23 females with fibromyalgia (according to the American College of Rheumatology classification criteria) and 20 healthy females. The results showed significantly higher serum concentrations of aspartate, cysteine, glutamate, glycine, isoleucine, leucine, methionine, ornithine, phenylalanine, sarcosine, serine, taurine, tyrosine and valine in FMS patients vs...
April 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28071727/plasma-amino-acid-profile-in-patients-with-aortic-dissection
#12
Linlin Wang, Sha Liu, Wengang Yang, Haitao Yu, Li Zhang, Ping Ma, Peng Wu, Xue Li, Kenka Cho, Song Xue, Baohong Jiang
Aortic dissection (AD), a severe cardiovascular disease with the characteristics of high mortality, is lack of specific clinical biomarkers. In order to facilitate the diagnosis of AD, we investigated plasma amino acid profile through metabolomics approach. Total 33 human subjects were enrolled in the study: 11 coronary heart disease (CHD) patients without aortic lesion and 11 acute AD and 11 chronic AD. Amino acids were identified in plasma using liquid chromatography and mass spectrometry (LC-MS/MS), and were further subjected to multiple logistic regression analysis...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28031282/tonb-dependent-heme-hemoglobin-utilization-by-caulobacter-crescentus-huta
#13
Heloise Balhesteros, Yan Shipelskiy, Noah Long, Aritri Majumdar, Benjamin B Katz, Naara M Santos, Laura Leaden, Salete M Newton, Marilis V Marques, Phillip E Klebba
: Siderophore nutrition tests with Caulobacter crescentus strain NA1000 revealed that it utilized a variety of ferric hydroxamate siderophores, including asperchromes, ferrichromes, ferrichrome A, malonichrome and ferric aerobactin, as well as hemin and hemoglobin. C. crescentus did not transport ferrioxamine B or ferric catecholates. Because it did not use ferric enterobactin, the catecholate aposiderophore was an effective agent for iron deprivation. We determined the kinetics and thermodynamics of [(59)Fe]-apoferrichrome and [(59)Fe]-citrate binding and transport by NA1000...
December 28, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27999586/glyphosate-resistant-parthenium-hysterophorus-in-the-caribbean-islands-non-target-site-resistance-and-target-site-resistance-in-relation-to-resistance-levels
#14
Enzo Bracamonte, Pablo T Fernández-Moreno, Francisco Barro, Rafael De Prado
Glyphosate has been the most intensely herbicide used worldwide for decades, and continues to be a single tool for controlling weeds in woody crops. However, the adoption of this herbicide in a wide range of culture systems has led to the emergence of resistant weeds. Glyphosate has been widely used primarily on citrus in the Caribbean area, but a study of resistance in the Caribbean islands of Cuba and the Dominican Republic has never been carried out. Unfortunately, Parthenium hysterophorus has developed glyphosate-resistance in both islands, independently...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27998432/-study-on-the-kidney-impairment-and-expressions-of-fgf21-from-a-rat-model-of-vascular-calcification
#15
Y C Shi, J H Liu
Objective: To investigate the injury and pathological changes of kidney in a rat model of aortic vascular calcification and to explore the expressions of fibroblast growth factor 21 (FGF21). Methods: A total of 14 Spraugue Dawley (SD) rats were randomly divided into two groups: control group and vitamin D3+ nicotine (VDN) group, with 7 rats in each group.The rats in VDN group received vitamin D3 and nicotine to induce vascular calcification.The content of serum creatinine was determined by sarcosine oxidase method...
December 13, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27942568/data-on-the-role-of-accessible-surface-area-on-osmolytes-induced-protein-stabilization
#16
Safikur Rahman, Syed Ausaf Ali, Asimul Islam, Md Imtaiyaz Hassan, Faizan Ahmad
This paper describes data related to the research article "Testing the dependence of stabilizing effect of osmolytes on the fractional increase in the accessible surface area on thermal and chemical denaturations of proteins" [1]. Heat- and guanidinium chloride (GdmCl)-induced denaturation of three disulfide free proteins (bovine cytochrome c (b-cyt-c), myoglobin (Mb) and barstar) in the presence of different concentrations of methylamines (sarcosine, glycine-betaine (GB) and trimethylamine-N-oxide (TMAO)) was monitored by [ϴ]222, the mean residue ellipticity at 222 nm at pH 7...
February 2017: Data in Brief
https://www.readbyqxmd.com/read/27934872/structural-analysis-of-glycine-sarcosine-n-methyltransferase-from-methanohalophilus-portucalensis-reveals-mechanistic-insights-into-the-regulation-of-methyltransferase-activity
#17
Yi-Ru Lee, Te-Sheng Lin, Shu-Jung Lai, Mu-Sen Liu, Mei-Chin Lai, Nei-Li Chan
Methyltransferases play crucial roles in many cellular processes, and various regulatory mechanisms have evolved to control their activities. For methyltransferases involved in biosynthetic pathways, regulation via feedback inhibition is a commonly employed strategy to prevent excessive accumulation of the pathways' end products. To date, no biosynthetic methyltransferases have been characterized by X-ray crystallography in complex with their corresponding end product. Here, we report the crystal structures of the glycine sarcosine N-methyltransferase from the halophilic archaeon Methanohalophilus portucalensis (MpGSMT), which represents the first structural elucidation of the GSMT methyltransferase family...
December 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27903454/effects-of-frequently-used-pharmaceutical-excipients-on-the-organic-cation-transporters-1-3-and-peptide-transporters-1-2-stably-expressed-in-mdckii-cells
#18
Marcus Otter, Stefan Oswald, Werner Siegmund, Markus Keiser
There is ample evidence that pharmaceutical excipients, which are supposed to be pharmacologically inactive, have an impact on drug metabolism and efflux transport. So far, little is known whether they also modulate uptake transporter proteins. We have recently shown that commonly used solubilizing agents exert significant effects on the function of organic anion uptake transporting polypeptides. Therefore, we investigated in this study the influence of frequently used pharmaceutical excipients on the transport activity of organic cation transporters OCT1, OCT2 and OCT3 and the peptide transporters PEPT1 and PEPT2...
November 26, 2016: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/27902449/is-n-n-dimethylglycine-n-oxide-a-choline-and-betaine-metabolite
#19
Michael Lever, Christopher J McEntyre, Peter M George, Stephen T Chambers
Choline metabolism is by oxidation to betaine, which is demethylated to N,N-dimethylglycine; dimethylglycine is oxidatively demethylated to sarcosine. This pathway is important for osmoregulation and as a source of methyl groups. We asked whether another metabolite was involved. We synthesized the N-oxide of dimethylglycine (DMGO) by oxidizing dimethylglycine with peracetic acid, and measured DMGO in human plasma and urine by HPLC-MS/MS with positive ion detection, using two chromatography procedures, based on ion exchange and HILIC separations...
January 13, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/27877876/enhanced-cellular-uptake-of-lactosomes-using-cell-penetrating-peptides
#20
Akiya Akahoshi, Eiji Matsuura, Eiichi Ozeki, Hayato Matsui, Kazunori Watanabe, Takashi Ohtsuki
Polymeric micelles that are composed of synthetic polymers are generally size controllable and can be easily modified for various applications. Lactosomes (A3B-type) are biodegradable polymeric micelles composed of an amphipathic polymer, including three poly(sarcosine) blocks and a poly(l-lactic acid) block. Lactosomes accumulate in tumors in vivo through the enhanced permeability and retention (EPR) effect, even on frequently administering them. However, lactosomes cannot be efficiently internalized by cells...
2016: Science and Technology of Advanced Materials
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