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Dimethylglycine

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https://www.readbyqxmd.com/read/27902449/is-n-n-dimethylglycine-n-oxide-a-choline-and-betaine-metabolite
#1
Michael Lever, Christopher J McEntyre, Peter M George, Stephen T Chambers
Choline metabolism is by oxidation to betaine, which is demethylated to N,N-dimethylglycine; dimethylglycine is oxidatively demethylated to sarcosine. This pathway is important for osmoregulation and as a source of methyl groups. We asked whether another metabolite was involved. We synthesized the N-oxide of dimethylglycine (DMGO) by oxidizing dimethylglycine with peracetic acid, and measured DMGO in human plasma and urine by HPLC-MS/MS with positive ion detection, using two chromatography procedures, based on ion exchange and HILIC separations...
November 30, 2016: Biological Chemistry
https://www.readbyqxmd.com/read/27896396/metabolic-profiling-of-ob-ob-mouse-fatty-liver-using-hr-mas-1-h-nmr-combined-with-gene-expression-analysis-reveals-alterations-in-betaine-metabolism-and-the-transsulfuration-pathway
#2
Mikheil Gogiashvili, Karolina Edlund, Kathrin Gianmoena, Rosemarie Marchan, Alexander Brik, Jan T Andersson, Jörg Lambert, Katrin Madjar, Birte Hellwig, Jörg Rahnenführer, Jan G Hengstler, Roland Hergenröder, Cristina Cadenas
Metabolic perturbations resulting from excessive hepatic fat accumulation are poorly understood. Thus, in this study, leptin-deficient ob/ob mice, a mouse model of fatty liver disease, were used to investigate metabolic alterations in more detail. Metabolites were quantified in intact liver tissues of ob/ob (n = 8) and control (n = 8) mice using high-resolution magic angle spinning (HR-MAS) (1)H-NMR. In addition, after demonstrating that HR-MAS (1)H-NMR does not affect RNA integrity, transcriptional changes were measured by quantitative real-time PCR on RNA extracted from the same specimens after HR-MAS (1)H-NMR measurements...
November 28, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27824899/sarcosine-up-regulates-expression-of-genes-involved-in-cell-cycle-progression-of-metastatic-models-of-prostate-cancer
#3
Zbynek Heger, Miguel Angel Merlos Rodrigo, Petr Michalek, Hana Polanska, Michal Masarik, Vitezslav Vit, Mariana Plevova, Dalibor Pacik, Tomas Eckschlager, Marie Stiborova, Vojtech Adam
The effects of sarcosine on the processes driving prostate cancer (PCa) development remain still unclear. Herein, we show that a supplementation of metastatic PCa cells (androgen independent PC-3 and androgen dependent LNCaP) with sarcosine stimulates cells proliferation in vitro. Similar stimulatory effects were observed also in PCa murine xenografts, in which sarcosine treatment induced a tumor growth and significantly reduced weight of treated mice (p < 0.05). Determination of sarcosine metabolism-related amino acids and enzymes within tumor mass revealed significantly increased glycine, serine and sarcosine concentrations after treatment accompanied with the increased amount of sarcosine dehydrogenase...
2016: PloS One
https://www.readbyqxmd.com/read/27757515/high-throughput-and-simultaneous-quantitative-analysis-of-homocysteine-methionine-cycle-metabolites-and-co-factors-in-blood-plasma-and-cerebrospinal-fluid-by-isotope-dilution-lc-ms-ms
#4
Seu Ping Guiraud, Ivan Montoliu, Laeticia Da Silva, Loïc Dayon, Antonio Núñez Galindo, John Corthésy, Martin Kussmann, Francois-Pierre Martin
The methionine cycle is a key pathway contributing to the regulation of human health, with well-established involvement in cardiovascular diseases and cognitive function. Changes in one-carbon cycle metabolites have also been associated with mild cognitive decline, vascular dementia, and Alzheimer's disease. Today, there is no single analytical method to monitor both metabolites and co-factors of the methionine cycle. To address this limitation, we here report for the first time a new method for the simultaneous quantitation of 17 metabolites in the methionine cycle, which are homocysteic acid, taurine, serine, cysteine, glycine, homocysteine, riboflavin, methionine, pyridoxine, cystathionine, pyridoxamine, S-adenosylhomocysteine, S-adenosylmethionine, betaine, choline, dimethylglycine, and 5-methyltetrahydrofolic acid...
October 18, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27746335/nmr-based-metabonomics-study-on-the-effect-of-gancao-in-the-attenuation-of-toxicity-in-rats-induced-by-fuzi
#5
Bo Sun, Xubin Wang, Ruili Cao, Qi Zhang, Qiao Liu, Meifeng Xu, Ming Zhang, Xiangbo Du, Fangting Dong, Xianzhong Yan
ETHNOPHARMACOLOGICAL RELEVANCE: Fuzi, the processed lateral root of Aconitum carmichaelii Debeaux, is a traditional Chinese medicine used for its analgesic, antipyretic, anti-rheumatoid arthritis and anti-inflammation effects; however, it is also well known for its toxicity. Gancao, the root of Glycyrrhiza uralensis Fisch., is often used concurrently with Fuzi to alleviate its toxicity. However, the mechanism of detoxication is still not well clear. AIM OF THE STUDY: In this study, the effect of Gancao on the metabolic changes induced by Fuzi was investigated by NMR-based metabonomic approaches...
October 13, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27735840/maternal-folate-status-and-the-bhmt-c-716g-a-polymorphism-affect-the-betaine-dimethylglycine-pathway-during-pregnancy
#6
Jose M Colomina, Pere Cavallé-Busquets, Sílvia Fernàndez-Roig, Pol Solé-Navais, Joan D Fernandez-Ballart, Mónica Ballesteros, Per M Ueland, Klaus Meyer, Michelle M Murphy
: The effect of the betaine: homocysteine methyltransferase BHMT c.716G>A (G: guanosine; A: adenosine) single nucleotide polymorphism (SNP) on the BHMT pathway is unknown during pregnancy. We hypothesised that it impairs betaine to dimethylglycine conversion and that folate status modifies its effect. We studied 612 women from the Reus Tarragona Birth Cohort from ≤12 gestational weeks (GW) throughout pregnancy. The frequency of the variant BHMT c.716A allele was 30.8% (95% confidence interval (CI): 28...
October 9, 2016: Nutrients
https://www.readbyqxmd.com/read/27733523/perinatal-dietary-choline-deficiency-in-sows-influences-concentrations-of-choline-metabolites-fatty-acids-and-amino-acids-in-milk-throughout-lactation
#7
Austin T Mudd, Lindsey S Alexander, Stacey K Johnson, Caitlyn M Getty, Olga V Malysheva, Marie A Caudill, Ryan N Dilger
BACKGROUND: Choline is essential for synthesis of phospholipids, neurodevelopment, and DNA methylation. It is unknown whether dietary perinatal choline deficiency affects maternal milk composition. OBJECTIVE: We examined whether perinatal maternal dietary choline deficiency influences porcine-milk composition. METHODS: Yorkshire sows were fed choline-deficient (CD) or choline-sufficient (CS) gestation diets [544 or 1887 mg choline/kg dry matter (DM), respectively] from 65 d before to 48 h after parturition and then fed lactation diets (517 or 1591 mg choline/kg DM, respectively) through day 19 of lactation...
October 12, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27614103/nonalcoholic-steatohepatitis-is-associated-with-a-state-of-betaine-insufficiency
#8
Silvia Sookoian, Puneet Puri, Gustavo O Castaño, Romina Scian, Faridodin Mirshahi, Arun J Sanyal, Carlos J Pirola
BACKGROUND AND AIMS: Nonalcoholic fatty liver disease (NAFLD) develops from a complex process, which includes changes in the liver methylome. Betaine plays a pivotal role in the regulation of methylogenesis. We performed a two-stage case-control study, which included patients with biopsy-proven NAFLD to explore circulating levels of betaine and its association with the histological spectrum. We also explored the association between a missense rs1805074, p.Ser646Pro variant in DMGDH (dimethylglycine dehydrogenase mitochondrial) and NAFLD severity (n=390)...
September 10, 2016: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/27575396/metabolic-responses-to-metal-pollution-in-shrimp-crangon-affinis-from-the-sites-along-the-laizhou-bay-in-the-bohai-sea
#9
Lanlan Xu, Chenglong Ji, Jianmin Zhao, Huifeng Wu
Marine environment in the Laizhou Bay is potentially contaminated by metals from industrial discharges. In this study, metal concentrations in shrimps Crangon affinis indicated that two typical sites (S6283 and S5283) close to Longkou and Zhaoyuan cities along the Laizhou Bay have been contaminated by metals, including Cd, As, Cu, Ni, Co, and Mn. In particular, Cd and As were the main metal contaminants in S6283. In S5283, however, Cu was the most important metal contaminant. The metabolic responses in the shrimps indicated that the metal pollution in S6283 and S5283 induced disturbances in osmotic regulation and energy metabolism and reduced anaerobiosis, lipid metabolism, and muscle movement...
August 26, 2016: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/27569255/plasma-trimethylamine-n-oxide-following-supplementation-with-vitamin-d-or-d-plus-b-vitamins
#10
Rima Obeid, Hussain M Awwad, Susanne H Kirsch, Christiane Waldura, Wolfgang Herrmann, Stefan Graeber, Juergen Geisel
SCOPE: We compared the effect of supplementation with vitamin D + B or vitamin D on plasma trimethylamine N-oxide (TMAO) and choline metabolites. METHODS AND RESULTS: This is a randomized single-blinded nonplacebo-controlled study. Twenty-seven participants received 1200 IU vitamin D3 and 800 mg calcium, and 25 participants received additionally 0.5 mg folic acid, 50 mg B6, and 0.5 mg B12 for 1 year. Plasma homocysteine (Hcy), TMAO, and choline metabolites were measured at baseline and 12 months later...
August 28, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27562778/trimethylamine-n-oxide-and-its-biological-variations-in-vegetarians
#11
Rima Obeid, Hussain M Awwad, Markus Keller, Juergen Geisel
PURPOSE: Restriction of animal foods and choline may affect plasma trimethylamine-N-oxide (TMAO). In vegetarians, we investigated the association between TMAO concentrations and the strictness of the diet or sex. We also studied the biological variations of TMAO in vegans. METHODS: Concentrations of plasma TMAO and choline metabolites were measured in 38 vegans and 67 lacto-ovo-vegetarians (group 1: mean age ± SD = 50 ± 15 years). Group 2 consisted of 66 vegans (29...
August 25, 2016: European Journal of Nutrition
https://www.readbyqxmd.com/read/27517309/microwave-assisted-intramolecular-ullmann-diaryl-etherification-as-the-post-ugi-annulation-for-generation-of-dibenz-b-f-1-4-oxazepine-scaffold
#12
Jianyu Shi, Jinlong Wu, Chengsen Cui, Wei-Min Dai
A sequence of the Ugi four-component reaction (U-4CR) and microwave-assisted intramolecular Ullmann etherification has been established for efficient generation of a dibenz[b,f][1,4]oxazepine scaffold. The U-4CR, using 2-aminophenols and 2-bromobenzoic acids or 2-bromobenzaldehydes as the inputs, was carried out in MeOH at 50-60 °C for 2-3 days to form a collection of 22 linear products in 46-90% yields. A microwave-assisted intramolecular Ullmann etherification was then used to transform these acyclic U-4CR products into the cleft-shaped 6/7/6-fused tricyclic heterocycles...
August 12, 2016: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/27503542/an-isotope-dilution-capillary-electrophoresis-tandem-mass-spectrometry-ce-ms-ms-method-for-the-simultaneous-measurement-of-choline-betaine-and-dimethylglycine-concentrations-in-human-plasma
#13
Mauro Forteschi, Angelo Zinellu, Stefano Assaretti, Arduino A Mangoni, Gianfranco Pintus, Ciriaco Carru, Salvatore Sotgia
Plasma concentrations of choline, betaine, and dimethylglycine provide valuable information on the flow of methyl groups in key biological processes, particularly during folate deficiency states. We developed a new method to simultaneously measure these analytes in human plasma. Following sample deproteinization using acetonitrile, an aliquot was evaporated to dryness under vacuum to be then taken up by water. Finally, analytes were separated by capillary electrophoresis and detected by electrospray ionization triple-quadrupole mass spectrometry, in multiple reaction monitoring mode, using two stable isotope-labeled internal standards...
October 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27486859/structure-and-biochemical-properties-of-recombinant-human-dimethylglycine-dehydrogenase-and-comparison-to-the-disease-related-h109r-variant
#14
Peter Augustin, Altijana Hromic, Tea Pavkov-Keller, Karl Gruber, Peter Macheroux
: The human dimethylglycine dehydrogenase (hDMGDH) is a flavin adenine dinucleotide (FAD)- and tetrahydrofolate (THF)-dependent, mitochondrial matrix enzyme taking part in choline degradation, one-carbon metabolism and electron transfer to the respiratory chain. The rare natural variant H109R causes dimethylglycine dehydrogenase deficiency leading to increased blood and urinary dimethylglycine concentrations. A detailed biochemical and structural characterization of hDMGDH was thus far hampered by insufficient heterologous expression of the protein...
October 2016: FEBS Journal
https://www.readbyqxmd.com/read/27476103/biochemical-fingerprint-and-pharmacological-applications-of-barleria-noctiflora-l-f-leaves
#15
Sangilimuthu Alagar Yadav, Sathishkumar Ramalingam, Anitha Jebamalairaj, Ravi Subban, Karpagavalli Mennakshi Sundaram
BACKGROUND: Antioxidant and antihistamine agents from Barleria noctiflora L.f. as natural source due to the existing modern medicine give various adverse effects to overcome these problems with natural products. METHODS: B. noctiflora leaves extract was fractionated with column chromatography; the homogenized fractions were monitored with thin layer chromatography (TLC) and characterized by using UV-visible, FT-IR, 1H NMR, 13C NMR and mass spectrometry spectral studies...
August 2, 2016: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/27430289/dynamic-metabolic-profiling-of-urine-biomarkers-in-rats-with-alcohol%C3%A2-induced-liver-damage-following-treatment-with-zhi%C3%A2-zi%C3%A2-da%C3%A2-huang-decoction
#16
Li An, Qiaoling Lang, Wenbin Shen, Qingshui Shi, Fang Feng
Alcoholic liver disease (ALD) is a leading cause of liver‑associated morbidity and mortality. Zhi‑Zi‑Da‑Huang decoction (ZZDHD), a traditional Chinese medicine formula, has been frequently used to treat or alleviate the symptoms of the various stages of ALD. To identify metabolic changes and the ZZDHD mechanism of action on ALD, potential urine biomarkers involved in the effects of ZZDHD were identified. Additionally, dynamic metabolomic profiles were systematically analyzed using nuclear magnetic resonance (NMR) spectroscopy in conjunction with statistical analysis...
September 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27413747/high-glucose-induced-pc12-cell-death-by-increasing-glutamate-production-and-decreasing-methyl-group-metabolism
#17
Minjiang Chen, Hong Zheng, Tingting Wei, Dan Wang, Huanhuan Xia, Liangcai Zhao, Jiansong Ji, Hongchang Gao
Objective. High glucose- (HG-) induced neuronal cell death is responsible for the development of diabetic neuropathy. However, the effect of HG on metabolism in neuronal cells is still unclear. Materials and Methods. The neural-crest derived PC12 cells were cultured for 72 h in the HG (75 mM) or control (25 mM) groups. We used NMR-based metabolomics to examine both intracellular and extracellular metabolic changes in HG-treated PC12 cells. Results. We found that the reduction in intracellular lactate may be due to excreting more lactate into the extracellular medium under HG condition...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27379829/one-carbon-metabolites-and-telomere-length-in-a-prospective-and-randomized-study-of-b-and-or-d-vitamin-supplementation
#18
Irene Pusceddu, Markus Herrmann, Susanne H Kirsch, Christian Werner, Ulrich Hübner, Marion Bodis, Ulrich Laufs, Thomas Widmann, Stefan Wagenpfeil, Jürgen Geisel, Wolfgang Herrmann
BACKGROUND: Vitamin B deficiency is common in elderly people and has been associated with an increased risk of developing age-related diseases. B-vitamins are essential for the synthesis and stability of DNA. Telomers are the end caps of chromosomes that shorten progressively with age, and short telomers are associated with DNA instability. OBJECTIVE: In the present randomized intervention study, we investigated whether the one-carbon metabolism is related to telomere length, a surrogate marker for cellular aging...
July 5, 2016: European Journal of Nutrition
https://www.readbyqxmd.com/read/27367522/components-of-one-carbon-metabolism-other-than-folate-and-colorectal-cancer-risk
#19
Robin Myte, Björn Gylling, Jörn Schneede, Per Magne Ueland, Jenny Häggström, Johan Hultdin, Göran Hallmans, Ingegerd Johansson, Richard Palmqvist, Bethany Van Guelpen
BACKGROUND: Despite extensive study, the role of folate in colorectal cancer remains unclear. Research has therefore begun to address the role of other elements of the folate-methionine metabolic cycles. This study investigated factors other than folate involved in one-carbon metabolism, i.e., choline, betaine, dimethylglycine, sarcosine, and methionine and relevant polymorphisms, in relation to the risk of colorectal cancer in a population with low intakes and circulating levels of folate...
November 2016: Epidemiology
https://www.readbyqxmd.com/read/27338465/amino-acid-and-biogenic-amine-profile-deviations-in-an-oral-glucose-tolerance-test-a-comparison-between-healthy-and-hyperlipidaemia-individuals-based-on-targeted-metabolomics
#20
Qi Li, Wenbo Gu, Xuan Ma, Yuxin Liu, Lidan Jiang, Rennan Feng, Liyan Liu
Hyperlipidemia (HLP) is characterized by a disturbance in lipid metabolism and is a primary risk factor for the development of insulin resistance (IR) and a well-established risk factor for cardiovascular disease and atherosclerosis. The aim of this work was to investigate the changes in postprandial amino acid and biogenic amine profiles provoked by an oral glucose tolerance test (OGTT) in HLP patients using targeted metabolomics. We used ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry to analyze the serum amino acid and biogenic amine profiles of 35 control and 35 HLP subjects during an OGTT...
2016: Nutrients
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