Amandine Roux, Zaynab Alsalman, Tao Jiang, Jean-Christophe Mulatier, Delphine Pitrat, Elise Dumont, François Riobé, Natacha Gillet, Eric Girard, Olivier Maury
Crystallophores are lanthanide complexes that have demonstrated outstanding induction of crystallization for various proteins. This article explores the effect of tailored modifications of the crystallophore first generation and their impact on the nucleating properties, and protein crystal structures. Through high-throughput crystallization experiments and dataset analysis, we evaluated the effectiveness of these variants, in comparison to the first crystallophore generation G1. In particular, the V1variant, featuring a propanol pendant arm, demonstrated the ability to produce new crystallization conditions for the proteins tested (hen-egg white lysozyme, proteinase K and thaumatin)...
May 13, 2024: Chemistry: a European Journal