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fractionation of plant extract

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https://www.readbyqxmd.com/read/30120053/hplc-characterization-of-molluscicidal-component-of-tamarindus-indica-and-its-mode-of-action-on-nervous-tissue-of-lymnaea-acuminata
#1
Neelam Soni, Vinay Kumar Singh, D K Singh
BACKGROUND: Fasciolosis is a water-borne disease with gastropods snail (Lymnaea acuminata) act as key-link is still burden for mankind especially in developing countries. Snail control is one of the important tools to trim down the frequency of fasciolosis. OBJECTIVE: To evaluate the toxic effect and inhibitory potential of plant Tamarindus indica and their active constituent on the key enzyme of nervous tissue of snail L. acuminata. METHOD: The present study deals with the chromatographic isolation and identification of molluscicidal component from Tamarindus indica bark and its effects on enzymes activities of vector snail L...
August 14, 2018: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/30116728/combination-of-cloxacillin-and-essential-oil-of-melaleuca-armillaris-as-an-alternative-against-staphylococcus-aureus
#2
Daniel Buldain, Andrea V Buchamer, María L Marchetti, Florencia Aliverti, Arnaldo Bandoni, Nora Mestorino
The emergence of resistance to antibiotics has been favored by abuse in the application of antimicrobials in human and animal medicine. Essential oils are a great resource to deal with this crisis. Melaleuca armillaris belongs to the family of Myrtaceae, rich in species with essential oils. Plant extracts has shown antimicrobial activity in many investigations. Cloxacillin (CLOX) is an antibiotic widely used in veterinary medicine against Staphylococcus aureus . Our aim was to assess pharmacodynamic interaction established by combining essential oil of M...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/30116190/antibacterial-properties-of-medicinal-plants-from-pakistan-against-multidrug-resistant-eskape-pathogens
#3
Muhammad Faraz Khan, Huaqiao Tang, James T Lyles, Rozenn Pineau, Zia-Ur-Rahman Mashwani, Cassandra L Quave
Local people in the Sudhnoti district of Pakistan share a rich practice of traditional medicine for the treatment of a variety of ailments. We selected nine plants from the Sudhnoti ethnopharmacological tradition used for the treatment of infectious and inflammatory disease. Our aim was to evaluate the in vitro anti-infective potential of extracts from these species against multidrug-resistant (MDR) ESKAPE ( Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa , and Enterobacter species) pathogens...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/30114281/antihyperuricemic-and-xanthine-oxidase-inhibitory-activities-of-tribulus-arabicus-and-its-isolated-compound-ursolic-acid-in-vitro-and-in-vivo-investigation-and-docking-simulations
#4
Eman Abu-Gharbieh, Naglaa G Shehab, Ihab M Almasri, Yasser Bustanji
BACKGROUND: Hyperurecemia is usually associated with gout and various metabolic arthritis disorders. Limited medications are available to manage such conditions. This study aimed to isolate the triterpenes constituent of the plant and to assess xanthine oxidase (XO) inhibitory and antihyperuricemic activities of Tribulus arabicus ethanolic extract, its fractions and the isolated compound using in vitro and in vivo approaches. METHODS: The ethanolic extract, fractions; n-hexane, chloroform and n-butanol and the isolated compound (ursolic acid) were evaluated in vitro for their XO inhibitory activity...
2018: PloS One
https://www.readbyqxmd.com/read/30112157/tracking-leading-anti-candida-compounds-in-plant-samples-plumbago-europaea
#5
Marzieh Sobhani, Mahdi Abbas-Mohammadi, Samad Nejad Ebrahimi, Atousa Aliahmadi
Background and Objectives: Due to the importance of finding new and more effective antifungal and antibacterial compounds against invasive vaginitis strains, this study was conducted for fast screening of plant samples. Materials and Methods: Thirty Iranian plant samples were successively extracted by n -hexane, ethyl acetate and methanol to obtain a total of 90 extracts. Each extract was prepared in six concentrations and evaluated for antifungal activity via a micro-broth dilution method...
June 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/30110911/isolation-and-structural-characterization-of-bioactive-molecules-on-prostate-cancer-from-mayan-traditional-medicinal-plants
#6
Rafael Sebastián Fort, Juan M Trinidad Barnech, Juliette Dourron, Marcos Colazzo, Francisco J Aguirre-Crespo, María Ana Duhagon, Guzmán Álvarez
Prostate cancer is the most common cancer in men around the world. It is a complex and heterogeneous disease in which androgens and their receptors play a crucial role in the progression and development. The current treatment for prostate cancer is a combination of surgery, hormone therapy, radiation and chemotherapy. Therapeutic agents commonly used in the clinic include steroidal and non-steroidal anti-androgens, such as cyproterone acetate, bicalutamide and enzalutamide. These few agents have multiple adverse effects and are not 100% effective...
August 14, 2018: Pharmaceuticals
https://www.readbyqxmd.com/read/30102993/bioassay-guided-isolation-and-uhplc-dad-esi-ms-ms-quantification-of-potential-anti-inflammatory-phenolic-compounds-from-flowers-of-inula-montana-l
#7
Elnur Garayev, Carole Di Giorgio, Gaëtan Herbette, Fathi Mabrouki, Philippe Chiffolleau, David Roux, Huguette Sallanon, Evelyne Ollivier, Riad Elias, Béatrice Baghdikian
ETHNOPHARMACOLOGICAL RELEVANCE: Flowers of Inula montana L. (Asteraceae), commonly known as "Arnica de Provence", are used in the traditional medicine of Provence in France with the same indication as Arnica montana, for the relief of bruises, as an anti-inflammatory agent. AIMS OF THE STUDY: The aim of our study is to evaluate its anti-inflammatory properties and to justify its traditional uses. Its potential valorization is evaluated in order to propose Inula montana as an alternative to Arnica montana...
August 10, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/30097123/bioactive-compound-1-8-cineole-selectively-induces-g2-m-arrest-in-a431-cells-through-the-upregulation-of-the-p53-signaling-pathway-and-molecular-docking-studies
#8
Sowndarya Sampath, Sangeetha Subramani, Sridevi Janardhanam, Preethi Subramani, Arun Yuvaraj, Rose Chellan
BACKGROUND: Callistemon citrinus has been traditionally known for its medicinal property. Recently, our research group identified 1,8-Cineole, as one of the predominant compound present in the hexane extract (HE-C), whose leaves have potent anticancer activity. HYPOTHESIS/PURPOSE: The present study was designed to isolate 1,8-Cineole from Callistemon citrinus plant and to determine their role in anticancer effects in in vitro using skin carcinoma cells. Moreover, the molecular mechanism of apoptosis and molecular docking studies were also investigated...
July 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/30097109/in-vitro-activity-of-collinin-isolated-from-the-leaves-of-zanthoxylum-schinifolium-against-multidrug-and-extensively-drug-resistant-mycobacterium-tuberculosis
#9
Sukyung Kim, Hoonhee Seo, Hafij Al Mahmud, Md Imtiazul Islam, Byung-Eui Lee, Myoung-Lae Cho, Ho-Yeon Song
BACKGROUND: Tuberculosis is a very serious infectious disease that threatens humanity, and the emergence of multidrug-resistant (MDR), extensively drug-resistant (XDR) strains resistant to drugs suggests that new drug development is urgent. In order to develop new tuberculosis drug, we have conducted in vitro anti-tubercular tests on thousands of plant-derived substances and finally found collinin extracted from the leaves of Zanthoxylum schinifolium, which has an excellent anti-tuberculosis effect...
July 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/30086508/phytochemical-profile-of-euphorbia-peplus-l-collected-in-central-italy-and-nmr-semi-quantitative-analysis-of-the-diterpenoid-fraction
#10
Claudio Frezza, Alessandro Venditti, Fabio Sciubba, Pierpaolo Tomai, Marika Antonetti, Marco Franceschin, Maria Enrica Di Cocco, Alessandra Gentili, Maurizio Delfini, Mauro Serafini, Armandodoriano Bianco
In this work, the phytochemical profile of the ethanolic extract of Euphorbia peplus L. collected in Central Italy, was reported. This specimen had never been studied before and the analysis was accomplished by means of Column Chromatography for the separation procedure and by means of NMR Spectroscopy and Mass Spectrometry for the identification step. In particular, fourteen compounds were evidenced belonging to five different classes of natural compounds i.e. triterpenoids (pentacyclic and saponin), peculiar diterpenoids (jatrophanes and pepluanes), flavonoids (flavonols), caffeoyl-quinic acids and rare disaccharides...
July 31, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30083230/lavender-and-lavandin-distilled-straws-an-untapped-feedstock-with-great-potential-for-the-production-of-high-added-value-compounds-and-fungal-enzymes
#11
Laurence Lesage-Meessen, Marine Bou, Christian Ginies, Didier Chevret, David Navarro, Elodie Drula, Estelle Bonnin, José C Del Río, Elise Odinot, Alexandra Bisotto, Jean-Guy Berrin, Jean-Claude Sigoillot, Craig B Faulds, Anne Lomascolo
Background: Lavender ( Lavandula angustifolia ) and lavandin (a sterile hybrid of L. angustifolia  ×  L. latifolia ) essential oils are among those most commonly used in the world for various industrial purposes, including perfumes, pharmaceuticals and cosmetics. The solid residues from aromatic plant distillation such as lavender- and lavandin-distilled straws are generally considered as wastes, and consequently either left in the fields or burnt. However, lavender- and lavandin-distilled straws are a potentially renewable plant biomass as they are cheap, non-food materials that can be used as raw feedstocks for green chemistry industry...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/30079972/novel-types-of-hypermodified-fluorescent-phyllobilins-from-breakdown-of-chlorophyll-in-senescent-leaves-of-grapevine-vitis-vinifera
#12
Theresia Erhart, Cecilia Mittelberger, Xiujun Liu, Maren Podewitz, Chengjie Li, Gerhard Scherzer, Gertrud Stoll, Josep Valls, Peter Robatscher, Klaus Liedl, Michael Oberhuber, Bernhard Kräutler
The tetrapyrrolic chlorophyll catabolites (or phyllobilins, PBs) were analyzed in yellow fall leaves of the grape Chardonnay, a common Vitis vinifera white wine cultivar. The major fractions in leaf extracts of V. vinifera, tentatively assigned to PBs, were isolated and their structures elucidated. The dominant fraction is a dioxobilin-type non-fluorescent Chl-catabolite of a previously observed type. Two less polar fluorescent PBs were characterized as a novel dioxobilin-type fluorescent Chl-catabolite with a bicyclo-1',6'-glycosyl architecture, and its new fluorescent formyloxobilin-type analog...
August 6, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/30079073/biostimulant-potential-of-humic-acids-extracted-from-an-amendment-obtained-via-combination-of-olive-mill-wastewaters-omw-and-a-pre-treated-organic-material-derived-from-municipal-solid-waste-msw
#13
Giuseppe Palumbo, Michela Schiavon, Serenella Nardi, Andrea Ertani, Giuseppe Celano, Claudio M Colombo
Olive mill wastewaters (OMW) contain significant levels of phenolic compounds with antimicrobial/phytotoxic activity and high amounts of undecomposed organic matter that may exert negative effects on soil biology. Among OMW detoxification techniques, those focusing on oxidative degradation of phenolic compounds are relevant. The composting (bio-oxidation) process in particular, exploits exothermic oxidation reactions by microorganisms to transform the organic matrix of OMW into an amendment biologically stable and feasible to use in agriculture...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/30066748/isoflavonoid-composition-and-biological-activity-of-extracts-from-soybean-seedlings-treated-by-different-elicitors
#14
Diego Durango, Jennifer Murillo, Fernando Echeverri, Gustavo Escobar, Winston Quiñones
Time-course and dose-response experiments were carried out to establish the ability for synthesizing isoflavonoids of soybean seedlings (cv. Soyica P34) treated with salicylic (SA) and isonicotinic acids (INA). Then, 25 structurally-related compounds were evaluated for their isoflavonoid-eliciting activity. Next, the antimicrobial and antioxidant activities of EtOAc-soluble fraction from soybean seedlings treated with some synthetic elicitors were determined. Results showed that the concentration of isoflavonoids in soybean seedlings was significantly increased by the application of SA and INA...
July 26, 2018: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/30065776/image-based-methods-for-phenotyping-growth-dynamics-and-fitness-components-in-arabidopsis-thaliana
#15
François Vasseur, Justine Bresson, George Wang, Rebecca Schwab, Detlef Weigel
Background: The model species Arabidopsis thaliana has extensive resources to investigate intraspecific trait variability and the genetic bases of ecologically relevant traits. However, the cost of equipment and software required for high-throughput phenotyping is often a bottleneck for large-scale studies, such as mutant screening or quantitative genetics analyses. Simple tools are needed for the measurement of fitness-related traits, like relative growth rate and fruit production, without investment in expensive infrastructures...
2018: Plant Methods
https://www.readbyqxmd.com/read/30065174/isolation-and-purification-of-potent-growth-inhibitors-from-piper-methysticum-root
#16
Truong Mai Van, Tran Dang Xuan, Truong Ngoc Minh, Nguyen Van Quan
Piper methysticum (kava) root is known to possess promising weed suppressing activity. The present study was conducted to search for potent plant growth inhibitors from the root of this medicinal pepper plant. The ethyl acetate (EtOAc) extract exhibited the strongest reduction on growth of Raphanus sativus (radish) (IC50 shoot and root growth = 172.00 and 51.31 µg/mL respectively) among solvent extracts. From this active extract, nine potent growth inhibitors involved in the inhibitory activities of P. methysticum root were isolated, purified and characterized by column chromatography (CC), gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR)...
July 31, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30064105/bioactivity-of-humic-substances-and-water-extracts-from-compost-made-by-ligno-cellulose-wastes-from-biorefinery
#17
R Spaccini, V Cozzolino, V Di Meo, D Savy, M Drosos, A Piccolo
The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials. The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts...
July 25, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/30063999/identification-of-fukinolic-acid-from-cimicifuga-heracleifolia-and-its-derivatives-as-novel-antiviral-compounds-against-enterovirus-a71-infection
#18
Yipeng Ma, Wenjuan Cong, Hao Huang, Liang Sun, Anh Hung Mai, Kurt Boonen, Wahedi Maryam, Wim De Borggraeve, Guoan Luo, Qingfei Liu, Liliane Schoofs, Frank Van Kuppeveld, Johan Neyts, Carmen Mirabelli, Walter Luyten
Human enterovirus 71 (EV-A71) infections cause a wide array of diseases ranging from diarrhoea and rashes to hand-foot-and-mouth disease and, in rare cases, severe neurological disorders. No specific antiviral drug therapy is currently available. Extracts from seventy-five Chinese medicinal plants selected for antiviral activity based on the Chinese pharmacopeia and advice from traditional Chinese medicine clinicians were tested for activity against EV-A71. The aqueous extract of the rhizome of Cimicifuga heracleifolia (Sheng Ma) and Arnebia euchroma (Zi Cao) showed potent antiviral activity...
July 28, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/30061495/-asteriscus-graveolens-extract-in-combination-with-cisplatin-etoposide-doxorubicin-suppresses-lymphoma-cell-growth-through-induction-of-caspase-3-dependent-apoptosis
#19
Zainab Tayeh, Rivka Ofir
Chemotherapy drugs action against cancer is not selective, lead to adverse reactions and drug resistance. Combination therapies have proven more effective in defeating cancers. We hypothesize that plant extract/fraction contains many/several compounds and as such can target multiple pathways as cytotoxic agent and may also have chemo sensitizing activities. We designed a study in which, Asteriscus graveolens ( Forssk. ) Less ( A. graveolens )-derived fraction that contains sesquiterpene lactone asteriscunolide isomers (AS) will be tested in combination with known chemotherapy drugs...
July 30, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30061386/unexpected-synergistic-hiv-neutralization-by-a-triple-microbicide-produced-in-rice-endosperm
#20
Evangelia Vamvaka, Gemma Farré, Luis M Molinos-Albert, Abbey Evans, Anna Canela-Xandri, Richard M Twyman, Jorge Carrillo, Raziel A Ordóñez, Robin J Shattock, Barry R O'Keefe, Bonaventura Clotet, Julian Blanco, Gurdev S Khush, Paul Christou, Teresa Capell
The transmission of HIV can be prevented by the application of neutralizing monoclonal antibodies and lectins. Traditional recombinant protein manufacturing platforms lack sufficient capacity and are too expensive for developing countries, which suffer the greatest disease burden. Plants offer an inexpensive and scalable alternative manufacturing platform that can produce multiple components in a single plant, which is important because multiple components are required to avoid the rapid emergence of HIV-1 strains resistant to single microbicides...
August 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
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