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fractionation of plant extract

Madamanchi Geethangili, Shih-Torng Ding
The genus Phyllanthus (L.) is one of the most important groups of plants belonging to the Phyllantaceae family. Phyllanthus urinaria (L.) is an annual perennial herbal species found in tropical Asia, America, China, and the Indian Ocean islands. P. urinaria is used in folk medicine as a cure to treat jaundice, diabetes, malaria, and liver diseases. This review provides traditional knowledge, phytochemistry, and biological activities of P. urinaria . The literature reviewed for this article was obtained from the Web of Science, SciFinder, PubMed, ScienceDirect, and Google Scholar journal papers published prior to December 2017...
2018: Frontiers in Pharmacology
María de la Luz Cádiz-Gurrea, Ignacio Fernández de Las Nieves, Luis Manuel Aguilera Saez, Salvador Fernández-Arroyo, Laurence Legeai-Mallet, Mohamed Bouaziz, Antonio Segura-Carretero
Plants, including most food and feed plants, produce a broad range of bioactive chemical compounds. Among these compounds, polyphenols are reported to provide beneficial effects as anti-carcinogenic, anti-atherogenic, anti-inflammatory, immune modulating, anti-microbial, vasodilatory and analgesic. Cocoa (Theobroma cacao), a major, economically important, international crop, has been related to several nutritional benefits, which have been associated with the phenolic fraction. The main subclass of flavonoids found in cocoa is flavanols, particularly (epi)catechins monomers, and their oligomers, also known as procyanidins...
October 15, 2018: Plant Foods for Human Nutrition
Nathiya Ranganathan, Gayathri Mahalingam
Endophytic fungi, especially from mangrove plants, are rich source of secondary metabolites, which plays a major role in various pharmacological actions preferably in cancer and bacterial infections. To perceive its role in antidiabetic activity we isolated and tested the metabolites derived from a novel strain Alternaria longipes strain VITN14G obtained from mangrove plant Avicennia officinalis. The crude extract was analyzed for antidiabetic activity and subjected to column chromatography. The isolated fractions were screened in vitro for α-glucosidase and α-amylase inhibitory activities...
October 15, 2018: Journal of Cellular Biochemistry
Amelia Torcello-Gómez, Mohamed A Gedi, Roger Ibbett, Khatija Nawaz Husain, Rhianna Briars, David Gray
An innovative procedure for plant chloroplasts isolation has been proposed, which consists of juice extraction by physical fractionation from plant material and recovery of its chloroplast-rich fraction (CRF) by centrifugation. This simple method has been applied to pea vine haulm subjected to different post-harvest treatments: blanching, storage at different relative humidity values and fermentation. Additionally, freeze storage of the extracted juice was carried out. The macronutrient (total lipids, proteins, ash and carbohydrates) and micronutrient (fatty acids, chlorophylls, β-carotene, α-tocopherol and ascorbic acid) content and composition of the CRF have been determined...
January 30, 2019: Food Chemistry
Meng Ge, Guijian Liu, Yuan Liu, Zijiao Yuan, Houqi Liu
A sediment core, spanning from the year 1925 ± 3.03 to 2012, was collected for analyzing historical distribution of mercury (Hg) in the Old Yellow River Estuary (OYRE), China. The Hg concentrations in the sediment core ranged from 14 to 351 ng g-1 , with the high values occurred in the middle layers (1960-1996), which may be resulted from intensive human activities during this period, such as oil exploitation and booming of petrochemical industry, salt chemical industry and chlor-alkali plants. A significant positive correlation was found between THg and loss on ignition (LOI)...
October 2018: Marine Pollution Bulletin
Tien-Jen Lin, Shu-Yi Yin, Pei-Wen Hsiao, Ning-Sun Yang, I-Jen Wang
The incidence of inflammatory bowel disease (IBD) has markedly increased. Our research findings during the past showed that medicinal plant extracts and the derived phytochemical components from Wedelia chinensis (WC) can have strong anti-colitis activities. Here, we further identified the key component phytochemicals from active fractions of different WC preparations (WCHA) that are responsible for the protective effect of WCHA in colitis mice. Of the 3 major compounds (wedelolactone, luteolin and apigenin) in this fraction, luteolin had the highest anti-inflammatory effect in vivo...
October 8, 2018: Scientific Reports
Rhea C Garcellano, Syed G A Moinuddin, Robert P Young, Mowei Zhou, Mark E Bowden, Ryan S Renslow, Yasemin Yesiltepe, Dennis G Thomas, Sean M Colby, Christopher D Chouinard, Gabe Nagy, Isaac K Attah, Yehia M Ibrahim, Rui Ma, Scott G Franzblau, Norman G Lewis, Alicia M Aguinaldo, John R Cort
A series of Wrightia hanleyi extracts was screened for activity against Mycobacterium tuberculosis H37Rv. One active fraction contained a compound that initially appeared to be either the isoflavonoid wrightiadione or the alkaloid tryptanthrin, both of which have been previously reported in other Wrightia species. Characterization by NMR and MS, as well as evaluation of the literature describing these compounds, led to the conclusion that wrightiadione (1) was misidentified in the first report of its isolation from W...
October 8, 2018: Journal of Natural Products
Nasima Khan, Farhana Afroz, Mst Nadira Begum, Satyajit Roy Rony, Suriya Sharmin, Fatema Moni, Choudhury Mahmood Hasan, Koushik Shaha, Md Hossain Sohrab
This study reports the chemical investigation and bioactivity of the secondary metabolites produced by the endophytic fungus Fusarium solani isolated from Cassia alata Linn. growing in Bangladesh. This plant was collected from conservation forest in Bangladesh and belongs to the Caesalpiniaceae family. The endophytic fungus Fusarium solani was isolated from the tissue of root of this plant. The fungal strain was identified by morphological characters and DNA sequencing. The crude organic extract of the fungal strain was proven to be active when tested for cytotoxicity against Brine Shrimp Lethality Bioassay, antimicrobial and antioxidant activity...
2018: Toxicology Reports
Graciela Granados-Guzmán, Rocío Castro-Ríos, Noemí Waksman de Torres, Ricardo Salazar-Aranda
Background: Microscale in vitro assays are fast, simple, and inexpensive, with reduced reagent quantities, waste, and experimental animal use. However, they have low reproducibility and low correlation with the results of in vivo models, possibly due to differences in precision and accuracy in methodologies between laboratories. Objective: The objective was the optimization and validation of an in vitro assay, carried out on microscale, to assess the inhibition of α-glucosidase activity, which is indicative of antihyperglycemic activity...
October 2018: Current Analytical Chemistry
Livia M Z G Passari, Ieda S Scarminio, Gustavo G Marcheafave, Roy E Bruns
Statistical mixture design extraction and fractionation of Mikania plant samples were carried out to quantitatively study seasonal and solvent composition effects as well as their interactions on secondary metabolites. The mixture design consisted of ethanol, acetone, dichloromethane and chloroform solvents and their binary, ternary and quaternary mixtures. Yields were measured for the crude extract and its neutral, organic, basic, polar and fiber fractions obtained with each solvent composition from samples harvested during 2010...
February 1, 2019: Food Chemistry
Aitebiremen Gift Omokhua, Muna Ali Abdalla, Johannes Van Staden, Lyndy Joy McGaw
BACKGROUND: Tithonia diversifolia and T. rotundifolia belong to the Asteraceae family and are native to Mexico and Central America. These plants have become invasive in parts of tropical Africa and Asia where they have become an ecological, agricultural and economic burden. Tithonia diversifolia is exploited by locals in its native and most parts of its invasive range as a source of medicines; however, T. rotundifolia is only used for medicinal purposes in one country in the native range (Venezuela) and none in the invasive range...
October 3, 2018: BMC Complementary and Alternative Medicine
Koichi Yada, Katsuhiko Suzuki, Natsumi Oginome, Sihui Ma, Youichi Fukuda, Akira Iida, Zsolt Radak
Endurance capacity is important for maintenance of quality of life as well as performance of endurance athletes. In order to improve endurance, intake of nutritional supplements as well as exercise training is also important. Indeed, polyphenolic extracts from plants are known to improve endurance capacity via increase of fatty acid utilization, mitochondrial biogenesis or inhibition of oxidative stress. Taheebo, the extract obtained from inner bark of Tabebuia avellanedae has been reported to have beneficial effects for treatment of inflammation, oxidative stress and obesity...
October 2, 2018: Scientific Reports
Larissa Marina Pereira Silva, Jovelina Samara Ferreira Alves, Emerson Michell da Silva Siqueira, Manoel André de Souza Neto, Lucas Silva Abreu, Josean Fechine Tavares, Dayanne Lopes Porto, Leandro de Santis Ferreira, Daniel Pecoraro Demarque, Norberto Peporine Lopes, Cícero Flávio Soares Aragão, Silvana Maria Zucolotto
Genipa americana is a medicinal plant popularly known as "jenipapo", which occurs in Brazil and belongs to the Rubiaceae family. It is a species widely distributed in the tropical Central and South America, especially in the Cerrado biome. Their leaves and fruits are used as food and popularly in folk medicine to treat anemias, as an antidiarrheal, and anti-syphilitic. Iridoids are the main secondary metabolites described from G. americana , but few studies have been conducted with their leaves. In this study, the aim was to chemical approach for identify the main compounds present at the extract of G...
October 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
T Jurca, I Baldea, G A Filip, D Olteanu, S Clichici, A Pallag, L Vicas, E Marian, O Micle, M Muresan
Tropaeolum majus L. (T. majus) or nasturtium is a medicinal plant widespread in the areas with temperate climate, commonly used in culinary and in traditional medicine due to therapeutic properties. In the last few years, various effects of the flowers and leaves of this plant have been studied, but their benefits are not fully known. The aim of the study was to identify the phenolic compounds from T. majus edible flowers in relation with its antioxidant capacity and the antimicrobial activity against different bacteria and Candida albicans...
June 2018: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
Najmeh Nasiri, Ghorbanali Nematzadeh, Vahid Shariati-Joni, Hamid Najafi-Zarrini
Premise of the Study: High-yield pure chloroplast DNA (cpDNA) is necessary for whole genome sequencing. Chloroplast extraction with traditional high-salt methods causes damage to nuclei and destroys the integrity of organelles, which leads to high genomic contamination from the nucleus and mitochondria. To overcome this issue, we modified a traditional high-salt method to obtain a new approach called the NaOH low-salt method (NLS). Methods and Results: The NLS method is based on the mild alkaline lysis of plant cells, followed by homogenization with ultrasonic waves and fractionation under reduced osmotic pressure...
September 2018: Applications in Plant Sciences
Yi Lei Ngo, Cher Haan Lau, Lee Suan Chua
Rosmarinic acid is a bioactive phytochemical that can be found in many herbs as ethnomedicines. It possesses remarkable pharmacological activities, and thus leading to its exploration as a therapeutic drug in diabetes treatment recently. This article reviews the extraction and fractionation techniques for plant-based natural rosmarinic acid and its anti-diabetic potential based on literature data published in journals, books, and patents from 1958 to 2017. Factors affecting the performance of rosmarinic acid extraction and fractionation such as operating temperature, time, solvent to sample ratio and eluent system are compiled and discussed in detail...
September 28, 2018: Food and Chemical Toxicology
Charles Kwaku Benneh, Robert Peter Biney, Donatus Wewura Adongo, Priscilla Kolibea Mante, Felix Agyei Ampadu, Augustine Tandoh, Jonathan Jato, Eric Woode
Introduction: The stem bark extract of Maerua angolensis DC. (Capparaceae) is used as a traditional remedy for management of anxiety, psychosis, and epilepsy. Aim of the Study: We therefore aimed at evaluating the anxiolytic and antidepressant potential of the plant in mice models. Methods: The dried stem bark was extracted with petroleum ether/ethyl acetate (50:50) mixture to obtain the extract, MAE. We employed Irwin's test to identify the preliminary behavioral and autonomic effects...
2018: Depression Research and Treatment
Seyed Ebrahim Sajjadi, Zeinab Delazari, Mahmoud Aghaei, Mustafa Ghannadian
Phlomis bruguieri (P. bruguieri) is a large genus in the Lamiaceae family, with a wide variety distributed in Euro-Asia, Central Asia, Iran, and China. Phlomis flowers have been used as herbal tea for gastrointestinal disturbances, protection of liver and cardiovascular systems. The aim of this study was to analyse phytochemical of flavonoid constituents in semi polar fraction of P. bruguieri . Methanol extract of plant material (4 kg) yielded 361 g dark green concentrated extract gum. After preliminary fractionation by normal column chromatography on silica gel, Fr...
October 2018: Research in Pharmaceutical Sciences
Ye Seul Lee, Yun Ju Lee, Soo Nam Park
Most people use cosmetics to protect their skin. Preservatives are often used to prevent their contamination upon use. There has been a great demand for natural preservatives due to recent reports on the side effects of parabens. Therefore, we evaluated the antimicrobial activities of Lonicera japonica and Magnolia obovata extracts and determined their potential as natural preservatives. We found that the 50% ethanol extract from L. japonica had antibacterial activity only against S. aureus and P. aeruginosa , while the ethyl acetate fraction showed antimicrobial activity against all six microbial strains tested...
September 20, 2018: Journal of Microbiology and Biotechnology
Muhammad Ibrar, Mir Azam Khan, Abdullah, Muhammad Imran
Paeonia emodi Wall. ex Royle is an important member of family Paeoniaceae and folklorically used for constipation, hysteria, respiratory diseases, epilepsy and cardiac diseases like hypertension, palpitations, congestive heart failure and atherosclerosis. In the present study, ethyl acetate fraction of P. emodi (Pe.EA) was subjected to column chromatography to obtain sub- fractions. These sub-fractions were screened for their cardioprotective activity in isoproterenol hydrochloride (ISO) induced myocardial infarction (MI) in mice...
September 22, 2018: Journal of Photochemistry and Photobiology. B, Biology
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