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"Blood" AND (Culture OR Cultures)

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https://www.readbyqxmd.com/read/30315942/manufacture-of-car-t-cells-from-mobilized-cyropreserved-peripheral-blood-stem-cell-units-depends-on-monocyte-depletion
#1
Annette Künkele, Christopher Brown, Adam Beebe, Stephanie Mgebroff, Adam J Johnson, Agne Taraseviciute, Lisa S Rolczynski, Cindy A Chang, Olivia C Finney, Julie R Park, Michael C Jensen
Cytotoxic chemotherapy and radiation can render lymphocyte repertoires qualitatively and quantitatively defective. Thus, heavily treated patients are often poor candidates for the manufacture of autologous CAR-T cell products. In the USA and Europe, children with high-risk neuroblastoma undergo apheresis early in the course of their treatment to collect peripheral blood stem cells (PBSCs) for cryopreservation in preparation for high-dose chemotherapy followed by autologous stem cell rescue. Here, we investigate whether these cryopreserved chemotherapy and G-CSF-mobilized PBSCs can be utilized as starting material for CAR-T cell manufacturing...
October 10, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/30315921/quantifying-the-importance-of-active-antimicrobial-therapy-among-patients-with-gram-negative-bloodstream-infections-cefepime-as-a-representative-agent
#2
C Miglis, N J Rhodes, J Liu, J Gener, E Hang, M Scheetz
The quantitative importance of active antibiotic treatment relative to other modifiable and non-modifiable risk factors for mortality has not been well defined in the literature. Here we quantify the impact of active antibiotic treatment on mortality relative to other disease modifiers in patients with Gram-negative bacteremia. Patients with at least one positive blood culture who were treated with ≥24 hours of cefepime for GNBSI were included for study. To examine in-hospital survival, a full primary model and a base model with the least significant covariate from the primary model were established...
October 10, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/30315869/validation-of-reference-genes-for-normalization-of-real-time-quantitative-pcr-studies-of-gene-expression-in-brain-capillary-endothelial-cells-cultured-in-vitro
#3
Maria Hersom, Charlotte Goldeman, Natasia Pretzer, Birger Brodin
BACKGROUND: The genes encoding β-actin and GAPDH are two of the most commonly used reference genes for normalization in in vitro blood-brain barrier studies. Studies have, however, shown that these reference genes might not always be the best choice. The aim of the present study was to evaluate 10 reference genes for use in mRNA profiling studies in primary cultures of brain endothelial cells of bovine origin. METHODS: Gene evaluations were performed by qPCR in mono-culture and in co-cultures with astrocytes...
October 10, 2018: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/30315825/tcf4-promotes-erythroid-development
#4
Florentien E M In 't Hout, Jolanda van Duren, Davide Monteferrario, Emma Brinkhuis, Niccolo Mariani, Theresia M Westers, Dana Chitu, Gorica Nikoloski, Arjan A van de Loosdrecht, Bert A van der Reijden, Joop H Jansen, Gerwin Huls
Transcription factor 4 (TCF4) is implicated in lymphoid cell differentiation and its expression predicts outcome in acute myeloid leukemia. Here, we investigated the role of TCF4 in myelopoiesis. Overexpression of TCF4 (TCF4OE) in umbilical cord blood (UCB) cells resulted in a 2-fold increase in erythroid colony forming units (CFU-Es), while knock-down of TCF4 (TCF4KD) caused a dramatic decrease in the number of erythroid colonies. In CFU-MKs, both TCF4KD and TCF4OE inhibited megakaryocyte colony formation...
October 10, 2018: Experimental Hematology
https://www.readbyqxmd.com/read/30315622/lessons-learned-from-a-hospital-wide-review-of-blood-stream-infections-for-paediatric-central-line-associated-blood-stream-infection-prevention
#5
Anita J Campbell, Christopher C Blyth, Christopher J Hewison, Yu-Ping Chen, Leanne Gough, Zoy Goff, Asha C Bowen
AIM: Bacteraemia episodes were assessed to calculate a hospital-wide central line-associated blood stream infection (CLABSI) rate per 1000 catheter-days. Secondary objectives were to describe risk factors, microbiology and outcomes of children with CLABSI. METHODS: A retrospective study was conducted at an Australian tertiary paediatric hospital in children <18 years who had blood culture sampling during the 2-year period, 2014-2015. All blood culture results were extracted from the hospital's laboratory information system...
October 13, 2018: Journal of Paediatrics and Child Health
https://www.readbyqxmd.com/read/30315547/tumor-metastasis-in-the-microcirculation
#6
Bingmei M Fu
Tumor cell metastasis through blood circulation is a complex process and is one of the great challenges in cancer research as metastatic spread is responsible for ∼90% of cancer-related mortality. Tumor cell intravasation into, arrest and adhesion at, and extravasation from the microvessel walls are critical steps in metastatic spread. Understanding these steps may lead to new therapeutic concepts for tumor metastasis. Vascular endothelium forming the microvessel wall and the glycocalyx layer at its surface are the principal barriers to and regulators of the material exchange between circulating blood and body tissues...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/30315486/can-excretory-phase-computed-tomography-predict-bacteremia-in-obstructive-calculous-pyelonephritis
#7
Yusuke Yagihashi, Shuichi Shimabukuro, Tomotune Toyosato, Yoshitaka Arakaki
PURPOSE: Acute pyelonephritis (AP), a complication of urolithiasis, can be fatal if it progresses to septic shock. We aimed to evaluate the performance of excretory phase computed tomography (CT) in predicting bacteremia among AP patients with upper urinary tract calculi. METHODS: We reviewed medical records of 250 patients diagnosed with AP and upper urinary tract calculi and who were admitted to our institute. We analyzed 132 patients who underwent excretory phase CT...
October 13, 2018: International Urology and Nephrology
https://www.readbyqxmd.com/read/30315454/aluminum-induced-electrophysiological-variation-synaptic-plasticity-impairment-and-related-mechanism
#8
Huifang Zhang
Aluminum, an environmentally abundant non-redox trivalent cation, has long been reported to alter blood-brain barrier and gets deposited in different regions of the brain. Many reports strongly indicated that Al had an adverse impact on the central nervous system (CNS), particularly on cognitive ability. Until now, studies in animal models and cell cultures have revealed that Al exposure results in altered behavioral performance and memory damage. The present paper reviews the scientific literature linking aluminum and the impairment of electrophysiological variation and synaptic plasticity...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/30315374/characterization-of-the-pathogenicity-of-clinical-cronobacter-malonaticus-strains-based-on-the-tissue-culture-investigations
#9
Abdlrhman M Alsonosi, Ondrej Holy, Stephen J Forsythe
Cronobacter malonaticus is a member of the genus Cronobacter which is considered an opportunistic pathogen. The significance of C. malonaticus has recently increased since it was documented to be involved in several serious neonatal infections. However, the virulence factors of C. malonaticus including their ability to adhere, invade and overcome host barriers have not been studied before. Unlike previous Cronobacter research, this study is mainly focused on C. malonaticus and is aimed to investigate its virulence characteristics that enable this species to cause adult and neonatal infections...
October 12, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/30314997/abnormally-expressed-mir-23b-in-chinese-mongolian-at-high-cardiovascular-risk-may-contribute-to-monocyte-macrophage-inflammatory-reaction-in-atherosclerosis
#10
Li-Ping He, Xing-Sheng Zhao, Le-Ping He
Background The prevalence of coronary heart disease (CHD) appears to be high among Chinese Mongolians. MiR-23b has been proven to play a key role in atherosclerosis. The expression and role of miR-23b in the Mongolians at high cardiovascular risk were explored in this study. Methods 40 cases of blood samples from the Mongolians at high cardiovascular risk were enrolled in this study. The expression of miR-23b was quantified by qRT-PCR. To induce monocytes differentiation into macrophages HP-1 cells were cultured with phorbol 12-myristate 13-acetate (PMA)...
October 12, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30314454/expression-of-fibronectin-in-esophageal-squamous-cell-carcinoma-and-its-role-in-migration
#11
Jiefei Xiao, Weilin Yang, Bo Xu, Haoshuai Zhu, Jianyong Zou, Chunhua Su, Jian Rong, Tao Wang, Zhenguang Chen
BACKGROUND: Fibronectin (FN) is a high-molecular-weight glycoprotein component of the extracellular matrix involved in cell adhesion, migration, metastasis, proliferation and differentiation, as well as embryogenesis, wound healing, and blood coagulation. Considerable recent research has established that tumor expression of FN is closely associated with tumor formation and development as well as disease prognosis. However, the mechanisms underlying this relationship have remained unclear...
October 12, 2018: BMC Cancer
https://www.readbyqxmd.com/read/30311873/rapid-identification-of-pathogens-from-positive-blood-culture-bottles-with-the-minion-nanopore-sequencer
#12
Sae Ashikawa, Norihito Tarumoto, Kazuo Imai, Jun Sakai, Masahiro Kodana, Toru Kawamura, Kenji Ikebuchi, Takashi Murakami, Kotaro Mitsutake, Shigefumi Maesaki, Takuya Maeda
PURPOSE: Bloodstream infections are major causes of morbidity and mortality that lead to prolonged hospital stays and higher medical costs. In this study, we aimed to evaluate the MinION nanopore sequencer for the identification of the most dominant pathogens in positive blood culture bottles. METHODOLOGY: 16S and ITS1-5.8S-ITS2 rRNA genes were amplified by PCR reactions with barcoded primers using nine clinical isolates obtained from positive blood bottles and 11 type strains, including five types of Candida species...
October 12, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30311842/effects-of-low-to-moderate-doses-of-gamma-radiation-on-mouse-hematopoietic-system
#13
XiangHong Li, Wanchang Cui, Lisa Hull, Joan T Smith, Juliann G Kiang, Mang Xiao
In this study, we investigated the effects of low-to-moderate doses of radiation in mice, given our limited understanding of the health risks associated with these exposures. Here, we demonstrate the different responses of the CD2F1 mouse hematopoietic system to low-to-moderate (0.5, 1, 3 or 5 Gy) doses of gamma radiation. After 3 and 5 Gy of 60 Co total-body irradiation (TBI), mouse blood cell counts were decreased and maintained below baseline up to 28-42 days. In contrast, after 0.5 Gy TBI, lymphocyte and monocyte counts increased, and peaked from day 3 to day 14...
October 12, 2018: Radiation Research
https://www.readbyqxmd.com/read/30311622/outbreak-of-brucella-melitensis-infection-in-eastern-sicily-risk-factors-clinical-characteristics-and-complication-rate
#14
Battistina A V Cama, Manuela Ceccarelli, Emmanuele Venanzi Rullo, Federica Ferraiolo, Ivana A Paolucci, Daniela Maranto, Placido Mondello, Maria R Lo Presti Costantino, Fernanda Marano, Giuseppa D'Andrea, Vincenzo Di Marco, Giovanni Puglisi, Mariella Valenzise, Gabriella D'Angelo, Lorenzo Mondello, Giuseppe Strano, Fabrizio Condorelli, Daria Spicola, Giuseppe Nunnari, Giovanni F Pellicanò
Brucellosis is one of the most common zoonoses in the world, especially in Southern Italy, where many cases are still recorded every year. 128 cases of brucellosis were observed in Messina (Sicily) in 2016, representing a tenfold increase in the number of cases of brucellosis expected. The aim of this multicenter retrospective study was to analyze clinical and microbiological aspects of a brucellosis outbreak in the province of Messina in 2016, the incidence of its complications and the treatment combinations applied...
October 12, 2018: New Microbiologica
https://www.readbyqxmd.com/read/30311254/generation-of-an-equine-biobank-to-be-used-for-functional-annotation-of-animal-genomes-project
#15
E N Burns, M H Bordbari, M J Mienaltowski, V K Affolter, M V Barro, F Gianino, G Gianino, E Giulotto, T S Kalbfleisch, S A Katzman, M Lassaline, T Leeb, M Mack, E J Müller, J N MacLeod, B Ming-Whitfield, C R Alanis, T Raudsepp, E Scott, S Vig, H Zhou, J L Petersen, R R Bellone, C J Finno
The Functional Annotation of Animal Genomes (FAANG) project aims to identify genomic regulatory elements in both sexes across multiple stages of development in domesticated animals. This study represents the first stage of the FAANG project for the horse, Equus caballus. A biobank of 80 tissue samples, two cell lines and six body fluids was created from two adult Thoroughbred mares. Ante-mortem assessments included full physical examinations, lameness, ophthalmologic and neurologic evaluations. Complete blood counts and serum biochemistries were also performed...
October 11, 2018: Animal Genetics
https://www.readbyqxmd.com/read/30310675/a-90-day-subchronic-toxicity-study-of-submerged-mycelial-culture-of-cordyceps-militaris-in-rats
#16
Bo-Yi Jhou, Wei-Chen Fang, Yen-Lien Chen, Chin-Chu Chen
Cordyceps militaris ( C. militaris ) is a parasitic fungus that grows on the larvae of Lepidoptera . It is a well-known fungus with immunomodulatory activity. The study was conducted to clarify the edible safety of C. militaris mycelium for long term use. Eighty Sprague-Dawley (SD) rats were divided into four groups (10 males and 10 females in each group). Rats were orally administrated with reverse osmosis water or 2000, 3000 and 4000 mg per kg BW per day freeze dried C. militaris mycelium powder for 90 consecutive days...
September 1, 2018: Toxicology Research
https://www.readbyqxmd.com/read/30310296/inhibitory-effects-of-fluoroquinolone-antibiotics-on-babesia-divergens-and-babesia-microti-blood-parasites-of-veterinary-and-zoonotic-importance
#17
Mohamed Abdo Rizk, Mahmoud AbouLaila, Shimaa Abd El-Salam El-Sayed, Azirwan Guswanto, Naoaki Yokoyama, Ikuo Igarashi
Aim: This study aimed to evaluate the inhibitory effects of fluoroquinolone antibiotics, including enrofloxacin, enoxacin, trovafloxacin, norfloxacin, and ofloxacin, on the in vitro and in vivo growth of Babesia divergens and Babesia microti parasites, respectively. Materials and methods: The in vitro and in vivo inhibitory effects of fluoroquinolone antibiotics against B. divergens and B. microti , respectively were evaluated using fluorescence-based assay. Additionally, combination therapies of highly effective fluoroquinolone antibiotics (enrofloxacin, enoxacin, and trovafloxacin) with diminazene aceturate, luteolin, or pyronaridine tetraphosphate were tested on the in vitro cultures of B...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/30310264/microbiological-study-of-dacryocystitis-in-northwest-of-iran
#18
Fatemeh Eslami, Hamid Reza Ghasemi Basir, Abbas Moradi, Shokoufe Heidari Farah
Background: The prevalence of bacterial pathogens isolated from patients with acute and chronic dacryocystitis and the pattern of antibiotic susceptibility tests are varied in each region. Moreover, the management of dacryocystitis is only based on clinical observations without microbiological evaluation. The present study aimed to identify epidemiologic and etiologic factors of dacryocystitis in our geographical area. Materials and methods: This cross-sectional and analytical study was carried out in the ophthalmology department of Hamadan University of Medical Sciences (northwest of Iran) during 2016-2017...
2018: Clinical Ophthalmology
https://www.readbyqxmd.com/read/30310171/association-of-histamine-with-hypertension-induced-cardiac-remodeling-and-reduction-of-hypertrophy-with-the-histamine-2-receptor-antagonist-famotidine-compared-with-the-beta-blocker-metoprolol
#19
Ajay Godwin Potnuri, Lingesh Allakonda, Arulvelan Appavoo, Sherin Saheera, Renuka R Nair
The association of histamine with adverse cardiac remodeling in chronic pressure overload has not received much attention. A pilot study in spontaneously hypertensive rats (SHRs) indicated a reduction of left ventricular hypertrophy (LVH) with a histamine-2-receptor (H2R) antagonist (famotidine). This finding prompted a detailed investigation of temporal variation in myocardial histamine and H2R expression and the cardiovascular response to H2R antagonism compared with that of the conventional beta-blocker metoprolol...
October 11, 2018: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/30310004/-surgical-treatment-of-acute-infective-endocarditis-after-transcatheter-aortic-valve-replacement
#20
Shunsuke Ohori, Kishu Fujita, Kiyotaka Morimoto, Masato Suzuki, Toshio Baba, Hideo Yokoyama, Fumikazu Nomura, Youhei Ohkawa, Kenji Sugiki, Takemi Ohno
Infective endocarditis (IE) after transcatheter aortic valve replacement (TAVR) is a rare complication, but has a high mortality. An 86-year-old female with symptomatic severe aortic stenosis underwent TAVR at our hospital and she was discharged without complication after 10 days. She was readmitted with high fever and acute heart failure 1 month later. Blood culture revealed Staphylococcus, and echocardiography showed vegetation on the septal cusp of the tricuspid valve and perforation at the membranous ventricular septum...
October 2018: Kyobu Geka. the Japanese Journal of Thoracic Surgery
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