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"Blood" AND (Culture OR Cultures)

Yichao Jin, Zhen Guan, Xiaochuan Wang, Zhiqiong Wang, Rong Zeng, Liangheng Xu, Ping Cao
5-Aminolevulinic acid photodynamic therapy(ALA-PDT) has been widely used for cervical cancer treatment, but the mechanisms are still not fully delineated. Here we showed that ALA-PDT significantly upregulated HMGB1 while downregulated miR-34a expression levels in cervical cancer tissues, and the percentages of mature DCs(mDCs) were increased in ALA-PDT treated patients' peripheral blood. After treating HPV-positive Hela, SiHa, Caski and HPV-negative C33 A cervical cancer cell lines with ALA-PDT, HPV-positive cells' proliferative ability was significantly inhibited and apoptosis rates were elevated, while no significant changes were found in HPV-negative C33 A cell line...
August 14, 2018: Photodiagnosis and Photodynamic Therapy
Pietro Badagliacca, Francesco Pomilio, Bruna Auricchio, Anna Franca Sperandii, Andrea Di Provvido, Mauro Di Ventura, Giacomo Migliorati, Mario Caudullo, Daniela Morelli, Fabrizio Anniballi
This report describes an outbreak of botulism occurred among a free-living population of mallards (Anas platyrhynchos) and geese (Anser anser) in an urban park. Mortality rate among investigated population was 86,8% (118 dead out of 136). Twenty-seven carcasses were collected for macroscopic examination and screened for microbiological, virological, toxicological investigations. A sick mallard was captured and neurological symptoms were observed. No causative agent of viral avian diseases was found in the examined animals and screening for environmental neurotoxic substances proved negative as well...
August 14, 2018: Anaerobe
Jing Wang, Run-Zhen He, Ge-Ling Lu, Heng-Li Luo, Dan-Qi Lu, An-Xing Li
To effectively increase production and improve economic returns, the co-culture of Nile tilapia (Oreochromis niloticus) and marine shrimp has been adopted in many countries, including China. Although O. niloticus is an euryhaline fish that can tolerate elevated salinities and even full-strength seawater, fluctuations in salinity levels can undoubtedly induce stress and affect the immune response of this fish. Therefore, this study assessed the impact of salinity on vaccine efficacy in Nile tilapia, which used serum antibody level as a surrogate marker to detect vaccine efficacy...
August 14, 2018: Fish & Shellfish Immunology
Maria Espejo, Shirley Magabo, Angel Rivera-Castro, Mohammed Faiz, Leandro Ramirez, Cristabel Robles, Tarek Shabarek, Masood A Shariff, Balavenkatesh Kanna
OBJECTIVE: To study the knowledge, perception, and behaviors among hypertensive African-Americans in South Bronx, New York, to elucidate any gaps that could explain their poor blood pressure control. METHODS: Cross-sectional qualitative study on African-American participants with essential hypertension, on single or combined oral antihypertensive regimen. Three focus groups were presented with open-ended questions on topics including cardiovascular disease knowledge, perception, and behaviors...
August 16, 2018: Journal of Racial and Ethnic Health Disparities
Logan L Vincent, Catherine M Otto
PURPOSE OF REVIEW: Infective endocarditis remains a highly mortal disease, yet the diagnosis often is missed or made only late in the disease course. The spectrum of patients presenting with endocarditis is shifting with different risk factors, predisposing patient characteristics, and treatment recommendations than in the past. This review addresses changes in the epidemiology of infective endocarditis, along with current diagnostic imaging modalities, treatment recommendations, and mortality trends...
August 16, 2018: Current Cardiology Reports
Qian Du, Chak Kwan Tong
A 41-year-old man from the emergency department presented with fever for 2 weeks, sore throat, dry cough and generalized umbilicated skin lesions (face (Fig. 1), and chest (Fig. 2)). HIV antibody was positive, CD4+ count was 2/μL. His skin swab, sputum and blood culture all yielded Talaromyces ( Penicillium ) marneffei (Fig. 3). Talaromyces marneffei is an important cause of morbidity and mortality in HIV-infected and other immunosuppressed patients who live in or are from endemic areas especially Southeast Asia...
2018: IDCases
Marilena Greco, Claudio Palumbo, Fernando Sicuro, Giambattista Lobreglio
Background: Sepsis is currently defined as a life-threatening organ dysfunction caused by a deregulated host response to infection. There is increasing evidence that the endothelium plays a crucial and pathogenic role in sepsis. Profound alterations of the endothelium associated with sepsis include increased leucocytes adhesions, shift to a procoagulant state, vasodilatation, altered barrier function with more permeable capillaries and tissue edema. The vascular endothelial growth factor (VEGF) pathway is involved in the control of microvascular permeability and has been involved in the pathogenesis of conditions associated with endothelial barrier disruption such as sepsis...
September 2018: Journal of Clinical Medicine Research
Yohsuke Yanase, Akihito Ohkawa, Satomi Inoue, Yukihiro Niida
A 69-year-old man presented with abdominal pain. Enhanced computed tomography (ECT) showed abdominal aortic aneurysm (AAA) with vessel wall thickening. Follow-up ECT on day 3 of admission showed expansion of the AAA. Endovascular abdominal aortic repair (EVAR) was urgently performed. Since preoperative blood cultures revealed Campylobacter jejuni infection, the antibiotics imipenem/cilastatin were administered for five weeks, followed orally by Clarithromycin. The patient was discharged on postoperative day 45...
June 25, 2018: Annals of Vascular Diseases
Yanming Sun, Zhitao Yao, Zhihua Zhao, Haifeng Xiao, Mengting Xia, Xiaojun Zhu, Xuelu Jiang, Chuntao Sun
Ovarian cancer has the highest mortality rate and is the most common of all gynecologic malignancies. Novel treatments for ovarian cancer are urgently required to improve outcomes and the overall survival of patients. The present study investigated whether immunotherapy with natural killer (NK) cells affected the survival of mice with ovarian cancer. Results analysis identified adjunctive NK cells as a potential therapeutic method in ovarian cancer. Patient-derived ovarian cells were isolated, cultured and subsequently injected subcutaneously into immune deficient BALB/c-nude mice...
August 2018: Experimental and Therapeutic Medicine
Samane Hoseini-Shahrestanak, Nasrin Bazargan, Leila Rahimian, Maryam Nemati, Saeed Solaymani, Abdollah Jafarzadeh
Background: The imbalance between Th2 and Treg cells plays fundamental role in the pathogenesis of allergic asthma. The current study aimed at assessing the expression of some Th2 and Treg cell-related parameters in patients with allergic asthma. Material and Methods: The serum and peripheral blood mononuclear cell (PBMC) samples were collected from 30 patients with asthma and 36 healthy subjects. The serum levels of transforming growth factor (TGF)-β, interleukin (IL)-4, as well as the expression levels of GATA3 and FOXP3 genes in PBMCs were determined by the enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR), respectively...
January 2018: Tanaffos
Dragan Djordjevic, Goran Rondovic, Maja Surbatovic, Ivan Stanojevic, Ivo Udovicic, Tamara Andjelic, Snjezana Zeba, Snezana Milosavljevic, Nikola Stankovic, Dzihan Abazovic, Jasna Jevdjic, Danilo Vojvodic
Background: Neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR), and mean platelet volume-to-platelet count (MPV/PC) ratio are readily available parameters that might have discriminative power regarding outcome. The aim of our study was to assess prognostic value of these biomarkers regarding outcome in critically ill patients with secondary sepsis and/or trauma. Methods: A total of 392 critically ill and injured patients, admitted to surgical ICU, were enrolled in a prospective observational study...
2018: Mediators of Inflammation
M H García-Hernández, E Rodríguez-Varela, R E García-Jacobo, M Hernández-De la Torre, E E Uresti-Rivera, R González-Amaro, D P Portales-Pérez
STATEMENTS OF THE PROBLEM: Regulatory B (Breg) cells have a critical role in adipose tissue homeostasis, and although subtypes of Breg cells have been described, their contribution during obesity is unclear. Therefore, the levels of regulatory B cells in adipose tissue and peripheral blood samples drawn from individuals with overweight, obesity, and normal-weight were evaluated. METHODS: The percentages of Breg cells were analyzed by flow cytometry. The activity of Breg cells was assessed by measuring the release of IFN-γ in the supernatants of co-cultures of CD4+ T and regulatory B cells with an ELISA assay...
August 13, 2018: Obesity Research & Clinical Practice
Melissa B Manus, Gerald S Bloomfield, Ashley Sobel Leonard, Laura N Guidera, David R Samson, Charles L Nunn
Elevated blood pressure presents a global health threat, with rates of hypertension increasing in low and middle-income countries. Lifestyle changes may be an important driver of these increases in blood pressure. Hypertension is particularly prevalent in African countries, though the majority of studies have focused on mainland Africa. We collected demographic and health data from 513 adults living in a community in rural Madagascar. We used generalized linear mixed models to assess body mass index (BMI), age, sex, and attributes related to household composition and lifestyle as predictors of blood pressure and hypertension...
2018: PloS One
Noushin Pouryazdanpanah, Reza Vahidi, Shahriar Dabiri, Ali Derakhshani, Alireza Farsinezhad
BACKGROUND: The mesenchymal stem cells (MSCs) of peripheral blood (PB) have been recognized as a promising source for allogeneic cell therapy. The objective of the present study was to isolate and characterize MSCs derived from non-mobilized PB, and evaluate their differentiation potential. METHODS: The buffy coat mononuclear fractions of the PB were concentrated using the Ficoll-Paque density gradient centrifugation and were grown on primary and secondary culture media, respectively...
August 1, 2018: Archives of Iranian Medicine
Obiora Shedrach Ejiofor, Onyinye Mercy Ajunwa, Chijioke Elias Ezeudu, George Ogonna Emechebe, Kenneth Nchekwube Okeke, Christian Chukwuemeka Ifezulike, Ifeoma Mercy Ekejindu, Jude Nnaemeka Okoyeh, Eunice Ogonna Osuala, Angus Nnamdi Oli
Background: Neonatal infection refers to the infection of the newborn during the first twenty-eight days of life. It is one of the causes of infant morbidity and mortality worldwide. The aim of the study is to determine the relative contribution of the different pathogens to the overall disease burden. It will also determine the mechanisms of virulence of these pathogens that cause neonatal infections at Chukwuemeka Odumegwu Ojukwu University Teaching Hospital (COOUTH), Awka. Methods: Biological samples were collected from 30 neonates admitted at the special care baby unit (SCBU) of COOUTH and cultured using selective media and nutrient agar...
2018: Journal of Pathogens
Xiang Li, Zhu-Wei Zhang, Zhong Wang, Jin-Quan Li, Gang Chen
Stroke, also known as "cerebrovascular accident", is an acute cerebrovascular disease that is caused by a sudden rupture of blood vessels in the brain or obstruction of the blood supply by blockage of blood vessels, thus including hemorrhagic and ischemic strokes. The incidence of ischemic stroke is higher than that of hemorrhagic stroke, and accounts for 80% of the total number of strokes. However, the mortality rate of hemorrhagic stroke is relatively high. Internal carotid artery and vertebral artery occlusion and stenosis can cause ischemic stroke, and especially males over 40 years of age are at a high risk of morbidity...
April 2018: Medical Gas Research
Jenny Rubin, Sharmineh Mansoori, Kristin Blom, Malin Berglund, Lena Lenhammar, Claes Andersson, Angelica Loskog, Mårten Fryknäs, Peter Nygren, Rolf Larsson
Mebendazole (MBZ) was recently shown to induce a tumor suppressive M1 phenotype in THP-1 monocytes and macrophages. In the present study the immune effects of MBZ was further investigated using human peripheral blood mononuclear cells (PBMCs) co-cultured with tumour cells. The Biomap platform was used to screen for biomarkers induced from MBZ exposed co-cultures of T-cell receptor activated PBMCs, HT29 colon cancer cells and either human fibroblasts or human umbilical vein endothelial cells (HUVEC) cells. In these co-culture systems MBZ at 0...
July 20, 2018: Oncotarget
Zhihao Zhang, Ping He, Yan Zhou, Xuhua Xie, Shuying Feng, Changyu Sun
The aim of the present study was to investigate the anti-hepatitis B virus (HBV) effect of interferon (IFN)-thymosin α1 (TA1) in a transgenic Dunaliella salina (TDS) system in vitro and in vivo . The toxicity of TDS in the HepG2.2.15 cell line was assessed using an MTT assay. The effect of TDS on the secretion of HBV early antigen (HBeAg) and HBV surface antigen (HBsAg) in culture supernatants was measured using ELISA. In addition, HBV-DNA was analyzed using quantitative polymerase chain reaction. Drug treatment experiments were performed in vivo on ducks congenitally infected with duck HBV (DHBV)...
August 2018: Experimental and Therapeutic Medicine
Franz Ratzinger, Helmuth Haslacher, Thomas Perkmann, Matilde Pinzan, Philip Anner, Athanasios Makristathis, Heinz Burgmann, Georg Heinze, Georg Dorffner
Bacteraemia is a life-threating condition requiring immediate diagnostic and therapeutic actions. Blood culture (BC) analyses often result in a low true positive result rate, indicating its improper usage. A predictive model might assist clinicians in deciding for whom to conduct or to avoid BC analysis in patients having a relevant bacteraemia risk. Predictive models were established by using linear and non-linear machine learning methods. To obtain proper data, a unique data set was collected prior to model estimation in a prospective cohort study, screening 3,370 standard care patients with suspected bacteraemia...
August 15, 2018: Scientific Reports
Rafaella Sayuri Ioshino, Danilo Oliveira Carvalho, Isabel Cristina Santos Marques, Ediane Saraiva Fernandes, Margareth Lara Capurro, André Luis Costa-da-Silva
Aedes aegypti is the principal vector of the urban arboviruses and the blood ingestion is important to produce the eggs in this species. To analyze the egg production in Ae. aegypti , researchers frequently use small cages or Drosophila vials to collect eggs from gravid females. Although it is affordable, the setup is time- and space-consuming, mainly when many mosquitoes need to be individually analyzed. This study presents an easy, cheap, and space-saving method to perform individual oviposition assays in Ae...
August 15, 2018: Insects
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