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https://www.readbyqxmd.com/read/30553966/a-novel-in-vitro-co-culture-model-to-examine-contact-formation-between-astrocytic-processes-and-cerebral-vessels
#1
Shoji Kawauchi, Sayo Horibe, Naoto Sasaki, Ken-Ichi Hirata, Yoshiyuki Rikitake
Here, we developed a novel in vitro co-culture model, in which process-bearing astrocytes and isolated cerebral microvessels from mice were co-cultured. Astrocytes formed contacts with microvessels from both adult and neonatal mice. However, concentrated localization of the immunofluorescence signal for aquaporin-4 (AQP4) at contact sites between perivascular endfoot processes and blood vessels was only detected with neonatal mouse microvessels. Contact between astrocytic processes and microvessels was retained, whereas concentrated localization of AQP4 signal at contact sites was lost, by knockdown of dystroglycan or α-syntrophin, reflecting polarized localization of AQP4 at perivascular regions in the brain...
December 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/30553722/comparison-of-multiplex-real-time-polymerase-chain-reaction-with-serological-tests-and-culture-for-diagnosing-human-brucellosis
#2
Tuba Dal, Soner Sertan Kara, Aytekin Cikman, Cigdem Eda Balkan, Ziya Cibali Acıkgoz, Hasan Zeybek, Hakan Uslu, Rıza Durmaz
OBJECTIVE: Brucellosis is a zoonotic disease with various clinical presentations and early diagnosis is crucial to avoid severe complications. Due to limitations of conventional diagnostic methods, polymerase chain reaction (PCR) based approaches have gained importance in diagnosis.We aimed to evaluate diagnostic value of multiplex real time-PCR (mRT-PCR) in serum samples collected from brucellosis suspected patients by comparision sensitivity of mRT-PCR with those of conventional diagnostic methods...
December 12, 2018: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/30553186/primary-cultured-neuronal-networks-and-type-2-diabetes-model-mouse-fatty-liver-tissues-in-aqueous-liquid-observed-by-atmospheric-sem-asem-staining-preferences-of-metal-solutions
#3
Chikara Sato, Toshiko Yamazawa, Akiko Ohtani, Yuusuke Maruyama, Nassirhadjy Memtily, Mari Sato, Yuri Hatano, Takashi Shiga, Tatsuhiko Ebihara
Neural networking, including axon targeting and synapse formation, is the basis of various brain functions, including memory and learning. Diabetes-mellitus affects peripheral nerves and is known to cause fatty liver disease. Electron microscopy (EM) provides the resolution required to observe changes in fine subcellular structures caused by such physiological and pathological processes, but samples are observed in vacuum. Environmental capsule EM can directly observe cells in a more natural aqueous environment, but the size-limited capsules restrict cell culturability...
November 23, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30553073/early-response-of-c2c12-myotubes-to-a-sub-cytotoxic-dose-of-hemorrhagic-metalloproteinase-hf3-from-bothrops-jararaca-venom
#4
Milene C Menezes, Eduardo S Kitano, Verena C Bauer, Ana K Oliveira, Eduardo C Lopes, Milton Y Nishiyama, André Zelanis, Solange M T Serrano
Manifestations of local tissue damage, such as hemorrhage and myonecrosis, are among the most dramatic effects of envenomation by viperid snakes. Snake venom metalloproteinases (SVMPs) of the P-III class are main players of the hemorrhagic effect due to their activities in promoting blood vessel disruption. Hemorrhagic Factor 3 (HF3), a P-III class SVMP from Bothrops jararaca, shows a minimum hemorrhagic dose of 240 fmol on rabbit skin. The aim of this study was to assess the effects of a sub-cytotoxic dose of HF3 (50 nM) on the proteomic profile of C2C12 differentiated cells (myotubes) in culture, and on the peptidomic profile of the culture supernatant...
December 12, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/30552311/injury-factors-alter-mirnas-profiles-of-exosomes-derived-from-islets-and-circulation
#5
Qi Fu, Hemin Jiang, Zibin Wang, Xingyun Wang, Heng Chen, Ziyang Shen, Lei Xiao, Xirong Guo, Tao Yang
Islets damage is a major abnormality underling diabetes. Recent studies suggested the value of exosomes in diagnosis. This study aimed to investigate the impact of injury factors on the miRNA profiles of islet exosomes and determine whether circulating exosomal miRNAs is suitable as biomarkers of islets damage. Islets were isolated from ICR mice and induced injury in vitro by mixed cytokines (Tumor Necrosis Factor-α, Interleukin -1β and Interferon-γ) or streptozotocin (STZ), and exosomes were derived from the cultural supernatant...
December 14, 2018: Aging
https://www.readbyqxmd.com/read/30552277/supported-self-management-for-people-with-type-2-diabetes-a-meta-review-of-quantitative-systematic-reviews
#6
Mireille Captieux, Gemma Pearce, Hannah L Parke, Eleni Epiphaniou, Sarah Wild, Stephanie J C Taylor, Hilary Pinnock
OBJECTIVES: Self-management support aims to give people with chronic disease confidence to actively manage their disease, in partnership with their healthcare provider. A meta-review can inform policy-makers and healthcare managers about the effectiveness of self-management support strategies for people with type 2 diabetes, and which interventions work best and for whom. DESIGN: A meta-review of systematic reviews of randomised controlled trials (RCTs) was performed adapting Cochrane methodology...
December 14, 2018: BMJ Open
https://www.readbyqxmd.com/read/30552239/indigenous-health-worker-support-for-patients-with-poorly-controlled-type-2-diabetes-study-protocol-for-a-cluster-randomised-controlled-trial-of-the-mana-t%C3%A5-programme
#7
Vanessa Selak, Tereki Stewart, Yannan Jiang, Jennifer Reid, Taria Tane, Peter Carswell, Matire Harwood
INTRODUCTION: Type 2 diabetes mellitus (T2DM) and its complications are more common among Māori and Pacific people compared with other ethnic groups in New Zealand. Comprehensive and sustained approaches that address social determinants of health are required to address this condition, including culturally specific interventions. Currently, New Zealand has no comprehensive T2DM management programme for Māori or Pacific people. METHODS AND ANALYSIS: The Mana Tū programme was developed by a Māori-led collaborative of primary healthcare workers and researchers, and codesigned with whānau (patients and their families) in order to address this gap...
December 14, 2018: BMJ Open
https://www.readbyqxmd.com/read/30552202/-18-f-fdg-pet-ct-guided-treatment-duration-in-patients-with-high-risk-staphylococcus-aureus-bacteremia-a-proof-of-principle
#8
Marvin A H Berrevoets, Ilse J E Kouijzer, Kitty Slieker, Erik Aarntzen, Bart Jan Kullberg, Jaap Ten Oever, Chantal P Bleeker-Rovers
Objectives: Current guidelines recommend intravenous antibiotic therapy for at least 4 weeks in patients with high-risk Staphylococcus aureus bacteremia (SAB), because of the risk for metastatic infection. We evaluated the safety of shorter duration of treatment in patients with high-risk SAB without signs of metastatic infection at presentation, using standard 18 F-FDG PET/CT and echocardiography. Methods: Retrospective analyses were performed of patients with SAB admitted between 2013 and 2017 in two medical centers...
December 14, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/30552086/psychiatrists-attitudes-toward-disruptive-new-technologies-mixed-methods-study
#9
Alexis Bourla, Florian Ferreri, Laetitia Ogorzelec, Charles-Siegfried Peretti, Christian Guinchard, Stephane Mouchabac
BACKGROUND: Recent discoveries in the fields of machine learning (ML), Ecological Momentary Assessment (EMA), computerized adaptive testing (CAT), digital phenotype, imaging, and biomarkers have brought about a new paradigm shift in medicine. OBJECTIVE: The aim of this study was to explore psychiatrists' perspectives on this paradigm through the prism of new clinical decision support systems (CDSSs). Our primary objective was to assess the acceptability of these new technologies...
December 14, 2018: JMIR Mental Health
https://www.readbyqxmd.com/read/30551935/prevalence-of-known-risk-factors-for-type-2-diabetes-mellitus-in-multiethnic-urban-youth-in-edmonton-findings-from-the-why-act-now-project
#10
Fariba Kolahdooz, Forouz Nader, Maryam Daemi, Se Lim Jang, Nora Johnston, Sangita Sharma
OBJECTIVES: The Canadian Diabetes Risk Assessment Questionnaire (CANRISK) is a validated, evidence-based, self-administered tool to assess the risk for type 2 diabetes mellitus in multiethnic Canadian adults. Identifying individuals at high risk for type 2 diabetes allows early intervention that improves modifiable risk factors. This study examined the risk factors for type 2 diabetes in multiethnic urban youth in Edmonton, Alberta. METHODS: An interviewer-administered questionnaire was developed based on CANRISK variables, such as age, gender, ethnicity, family history of diabetes, medical history of high blood sugar or high blood pressure, anthropometric measurements, physical activity and dietary intake...
October 9, 2018: Canadian Journal of Diabetes
https://www.readbyqxmd.com/read/30551462/protective-effects-of-acanthopanax-senticosus-ligustrum-lucidum-combination-on-bone-marrow-suppression-induced-by-chemotherapy-in-mice
#11
Chunyun Wang, Hongbo Gao, Enbo Cai, Lianxue Zhang, Xiaoman Zheng, Shengbo Zhang, Nian Sun, Yan Zhao
This study investigated the combination effects of the Acanthopanax senticosus - Ligustrum lucidum (AS-LL) herb pair on bone marrow suppression caused by chemotherapy. A bone marrow suppression model was established by intraperitoneal injection (i.p.) of cyclophosphamide (CTX, 100 mg/kg). The changes in chemical composition between the AS-LL decocted together and single were analyzed, and their effects on the bone marrow nucleated cells, peripheral blood, thymus and spleen indices, in vitro hematopoietic cell culture, ELISA and cell cycle were detected...
January 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30551309/the-preparation-of-the-ordered-pores-colloidal-crystal-scaffold-and-its-role-in-promoting-growth-of-lung-cells
#12
Qing Ban, Wuya Chen, Shiwei Du, Huimin Wang, Jian Li, Rong You, Ying Jin, Yongyong Xue, Yan-Qing Guan
Lung is one of important organs and lung diseases seriously affect the health of human beings. In this study, chitosan and gelatin as natural biological macromolecules raw material for the synthesis of ordered colloidal crystal scaffolds (CCS), Fe3 O4 magnetic nanoparticles (MNPs) were used as pore-making for the first time. The pore-making agent were added into the hydrogels to synthesis the ordered (magnetic field) and disordered (no magnetic field) CCS. Collagen and basic fibroblast growth factor (bFGF) modified on the surface of CCS...
January 1, 2019: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30551230/distribution-of-late-onset-neonatal-sepsis-pathogens-differs-in-inpatient-and-outpatient-settings
#13
Celeste G Black, Larissa Tavares, Anna Stachel, Adam J Ratner, Tara M Randis
OBJECTIVE:  We sought to examine pathogen distribution and clinical presentation of late-onset sepsis (LOS) at an urban tertiary care center. STUDY DESIGN:  We performed a retrospective review of all culture-confirmed cases of LOS presenting to our institution from 2013 to 2017. Medical records were evaluated for demographic information, sepsis risk factors, encounter location, and clinical outcome. RESULTS:  We identified 97 cases of LOS, with a median age at diagnosis of 25 days...
December 14, 2018: American Journal of Perinatology
https://www.readbyqxmd.com/read/30550945/etiology-and-epidemiology-of-community-acquired-pneumonia-in-adults-requiring-hospital-admission-a-prospective-study-in-rural-central-philippines
#14
Socorro Lupisan, Akira Suzuki, Noel Macalalad, Rutchie Egos, Lydia Sombrero, Michiko Okamoto, Clyde Dapat, Melisa Mondoy, Hazel Galang, Vicente Francisco Froilan Zeta, Flora de la Pena, Vicente Romano, Remigio Olveda, Hitoshi Oshitani
BACKGROUND: Community-acquired pneumonia (CAP) is a common cause of morbidity and mortality among adults worldwide. However, the distribution of etiology of CAP varies from one country to another with limited data from rural areas. METHODS: A prospective hospital-based study on adult CAP was conducted in Leyte, Central Philippines from May 2010 to May 2012. Blood, sputum, and nasopharyngeal samples obtained from patients were used to identify pathogens using standard microbiological culture methods and PCR...
December 11, 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/30550595/genome-survey-sequencing-for-the-characterization-of-genetic-background-of-dracaena-cambodiana-and-its-defense-response-during-dragon-s-blood-formation
#15
Xupo Ding, Wenli Mei, Shengzhuo Huang, Hui Wang, Jiahong Zhu, Wei Hu, Zehong Ding, Weiwei Tie, Shiqing Peng, Haofu Dai
Dragon's blood collected from the genus Dracaena is used as a renowned traditional medicine in various cultures worldwide. However, the genetics of the genus Dracaena and the formation mechanism of dragon's blood remain poorly understood. Here, we generate the first draft genome reference assembly of an elite Chinese Dracaena species, Dracaena cambodiana, from next-generation sequencing data with 89.46× coverage. The reads were assembled into 2,640,704 contigs with an N50 length of 1.87 kb, and a 1.05 Gb assembly was finally assembled with 2,379,659 scaffolds...
2018: PloS One
https://www.readbyqxmd.com/read/30550234/comparison-of-pcr-fluorescent-in-situ-hybridization-and-blood-cultures-for-detection-of-bacteremia-in-children-and-adolescents-during-antibiotic-therapy
#16
Tomasz W Źródłowski, Danuta Jurkiewicz-Badacz, Agnieszka Sroka-Oleksiak, Dominika Salamon, Małgorzata Bulanda, Tomasz Gosiewski
The gold standard in microbiological diagnostics of bacteremia is a blood culture in automated systems. This method may take several days and has low sensitivity. New screening methods that could quickly reveal the presence of bacteria would be extremely useful. The objective of this study was to estimate the effectiveness of these methods with respect to blood cultures in the context of antibiotic therapy. Blood samples from 92 children with sepsis were analyzed. Blood cultures were carried out in standard automated systems...
2018: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/30549995/direct-ms-based-bacterial-identification-of-blood-cultures-comparison-of-an-in-house-tritonx-protocol-with-bruker-sepsityper
#17
Maximilian Kittel, Anna Greulich, Peter Findeisen, Verena Haselmann, Achim Burrer, Christian Willem, Thomas Miethke, Parviz Ahmad-Nejad, Michael Neumaier
No abstract text is available yet for this article.
October 31, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30549244/cell-templated-silica-microparticles-with-supported-lipid-bilayers-as-artificial-antigen-presenting-cells-for-t-cell-activation
#18
Brynn R Olden, Caleb R Perez, Ashley L Wilson, Ian I Cardle, Yu-Shen Lin, Bryan Kaehr, Joshua A Gustafson, Michael C Jensen, Suzie H Pun
Biomaterial properties that modulate T cell activation, growth, and differentiation are of significant interest in the field of cellular immunotherapy manufacturing. In this work, a new platform technology that allows for the modulation of various activation particle design parameters important for polyclonal T cell activation is presented. Artificial antigen presenting cells (aAPCs) are successfully created using supported lipid bilayers on various cell-templated silica microparticles with defined membrane fluidity and stimulating antibody density...
December 13, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/30549135/human-native-endocarditis-caused-by-streptococcus-canis-a-case-report
#19
Barbora Mališová, Petr Šantavý, Yvona Lovečková, Bořivoj Hladký, Iva Kotásková, Jiří Pol, Vladimír Lonský, Petr Němec, Tomáš Freiberger
We report a very rare case of Streptococcus canis native infective endocarditis in a 73-year-old woman living in close contact with her dog. Her echocardiography showed large calcifications in the mitral annulus, massive regurgitation below the posterior leaflet, and adjacent vegetation. Blood culture was positive for Streptococcus Lancefield group G. A coronary artery bypass and mitral valve replacement had to be done. Streptococcus canis was detected in a heart valve using a broad range PCR followed by 16S rRNA and confirmed by tuf gene sequencing, while tissue culture remained negative...
January 2019: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30548621/recipient-sepsis-caused-by-lactococcus-garvieae-contamination-of-platelets-from-a-donor-with-colon-cancer
#20
Shuichi Nakamura, Kenji Nakai, Momoko Sakata, Yoshio Nagaoka, Kozue Yoshida, Uichiro Katsumata, Toshimi Chiba, Keiji Matsubayashi
Lactococcus garvieae is a well-known fish pathogen that has low virulence in humans and is rarely isolated from the blood cultures of endocarditis patients. We describe herein the first reported case of transfusion-transmitted L. garvieae sepsis caused by a contaminated platelet concentrate from a donor who consumed raw octopus before blood donation. Retrospective examination of the laboratory results of the index donor revealed that his haemoglobin levels had been steadily decreasing, which led to the detection of a latent colon cancer...
December 12, 2018: Vox Sanguinis
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