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Xunhui Zhuo, Hongchao Sun, Zhi Zhang, Jiaqing Luo, Ying Shan, Aifang Du
Serologic tests are widely accepted and applied as means to detect anti- Toxoplasma gondii immunoglobulin G antibodies. In this study, recombinant matrix antigen (rMAG1) was induced by isopropyl-β-d-thiogalactoside and purified by nickel-nitrilotriacetic acid purification system. We then developed and optimized an indirect enzyme-linked immunosorbent assay (ELISA) through checkerboard assays using serial dilutions of antigens and sera to assess the potential use of rMAG1 in serologic detection of T. gondii infection in dogs...
June 2017: Journal of Parasitology
Kentaro Kato, Yuho Murata, Noriyuki Horiuchi, Atsuko Inomata, Mohamad Alaa Terkawi, Akiko Ishiwa, Yohsuke Ogawa, Shinya Fukumoto, Fumikazu Matsuhisa, Kenji Koyama
BACKGROUND: Toxoplasma gondii is a highly prevalent protozoan that can infect all warm-blooded animals, including humans. Its definitive hosts are Felidae and its intermediate hosts include various other mammals and birds, including pigs. It is found in the meat of livestock which is a major source of human infection. Hence the control of toxoplasmosis in pigs is important for public health. We previously showed that dextran sulfate (DS), especially DS10 (dextran sulfate MW 10 kDa), is effective against T...
March 9, 2016: Parasites & Vectors
M Saqib, M H Hussain, M S Sajid, M K Mansoor, M N Asi, A A K Fadya, A Zohaib, A U R Sial, G Muhammad, I Ullah
Toxoplasmosis is a serious threat for livestock in addition to being of zoonotic significance. In this study, serodiagnosis of equine toxoplasmosis was conducted in a randomly selected population from the 3 metropolises of Punjab, Pakistan. To this end, 272 draught equines were screened using a commercial latex agglutination assay kit. Association of probable risk factors of equine toxoplasmosis was also documented. A total of 91 (33.5%) equines were found sero-positive for Toxoplama (T.) gondii having antibody titers ranging between 1:32 to 1:612...
June 2015: Tropical Biomedicine
Fawzia H Toulah, Saedia A Sayed Al-Ahl, Dawlat M Amin, Mona H Hamouda
Toxoplama gondii (Apicomplexa: Coccidia), an obligatory intracellular parasite with a unique capacity to invade virtually all nucleated cell type from warm-blooded vertebrate hosts. Despite the efficiency with which Toxoplasma enters its host cell, it remains unresolved if invasion occurs by direct penetration of the parasite or through phagocytosis. In the present work, electron microscopic study was designed to examine the entry process of Toxoplasma (RH strain) into macrophages and non phagocytic-host cells (Hela cells) and to observe the ultrastructure changes associated with intracellular parasitism...
April 2011: Saudi Journal of Biological Sciences
Christian Kropf, Karim Debache, Christoph Rampa, Fabienne Barna, Michelle Schorer, Chad E Stephens, Mohamed A Ismail, David W Boykin, Andrew Hemphill
The impact of di-cationic pentamidine-analogues against Toxoplama gondii (Rh- and Me49-background) was investigated. The 72 h-growth assays showed that the arylimidamide DB750 inhibited the proliferation of tachyzoites of T. gondii Rh and T. gondii Me49 with an IC(50) of 0·11 and 0·13 μM, respectively. Pre-incubation of fibroblast monolayers with 1 μM DB750 for 12 h and subsequent culture in the absence of the drug also resulted in a pronounced inhibiton of parasite proliferation. However, upon 5-6 days of drug exposure, T...
February 2012: Parasitology
Michelle Igarashi, Dauton Luiz Zulpo, Ivo Alexandre Leme da Cunha, Luiz Daniel Barros, Vanessa Figueredo Pereira, Alessandra Taroda, Italmar Teodorico Navarro, Odilon Vidotto, Marilda Carlos Vidotto, Mark Christopher Jenkins, João Luis Garcia
TgROP2 is an intracellular protein associated with rhoptries of Toxoplama gondii and an antigen component of a candidate vaccine for toxoplasmosis. The purpose of the present study was to evaluate the efficacy of rTgROP2 to stimulate humoral and cellular immune responses in BALB/c mice via intranasal injection. TgROP2 partial coding sequence was (196-561) amplified by PCR from genomic T. gondii RH strain DNA and cloned into the pTrcHis expression vector. Escherichia coli Rosetta 2 cells transformed with pTrcHis-TgROP2 showed high levels (~1 mg...
October 2010: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
Harold Salant, Dan T Spira, Joseph Hamburger
The relative role of transmission of Toxoplasma gondii infection from cats to humans appears to have recently increased in certain areas. Large-scale screening of oocyst shedding in cats cannot rely on microscopy because oocyst identification lacks sensitivity and specificity, or on bioassays, which require test animals and weeks before examination. We compared a sensitive and species-specific coprologic-polymerase chain reaction (copro-PCR) for detection of T. gondii infected cats with microscopy and a bioassay...
May 2010: American Journal of Tropical Medicine and Hygiene
Merav Lidar, Noga Lipschitz, Pnina Langevitz, Ori Barzilai, Maya Ram, Bat-Sheba Porat-Katz, Christian Pagnoux, Philippe Guilpain, Renato Alberto Sinico, Antonella Radice, Nicola Bizzaro, Jan Damoiseaux, Jan Willem Cohen Tervaert, Javier Martin, Loïc Guillevin, Stefano Bombardieri, Yehuda Shoenfeld
In this study we assess the presence of antibodies against infectious agents as well as for a variety of autoantibodies in an attempt to establish associations between various vasculitides and infections in order to shed light on the etiopathogenesis of these diseases and perhaps implicate a potential cure. Sera from patients with Wegener's granulomatosis (WG), polyarteritis nodosa, microscopic polyangiitis, Churg Strauss, and giant cell arteritis were compared to healthy control sera. Serum samples were assessed, using the Bio-Rad BioPlex 2200, for the presence of Toxoplama gondii, cytomegalovirus (CMV), Epstein-Barr virus (EBV), Treponema pallidum, and Saccharomyces cerevisiae...
September 2009: Annals of the New York Academy of Sciences
Amalio Telenti
PURPOSE OF REVIEW: Clinical trials of CCR5 antagonists attest to their efficacy and tolerance in HIV treatment. However, there has been debate on their long-term safety because of the role of CCR5 in innate immunity. This review highlights gaps in our understanding of epidemiology of infections that are modulated by CCR5, in particular, in HIV-infected individuals. RECENT FINDINGS: In the mouse model, CCR5 has a role in the response against pathogens as diverse as Toxoplama gondii, West Nile virus, Mycobacterium tuberculosis, herpes simplex virus, Trypanosoma cruzi, Cryptococcus neoformans, Chlamydia trachomatis, Listeria, and plasmodia...
March 2009: Current Opinion in HIV and AIDS
Nilson Giraldi, Odilon Vidotto, Italmar Teodorico Navarro, João Luis Garcia, Liza Ogawa, Edmundo Kobylka
The occurrence of toxoplasmosis and enteroparasitosis was studied in 434 children from elementary schools in the rural and urban areas of Rolândia, Paraná State, Brazil. Sera and fecal samples from all the students were submitted to IFA for Toxoplasma gondii and coproparasitological tests, respectively. The children were tested by Amsler grid and 72 of them were examined for the presence of lesions compatible with ocular toxoplasmosis. Some variables were tested but none showed increased risk for toxoplasmosis...
May 2002: Revista da Sociedade Brasileira de Medicina Tropical
Yan Ma, Tao Jin, Liyuan Wang, Tingzhong Yang, Lu Li, Le Zhang
OBJECTIVE: To explore the behavioral risk of toxoplasma infection in special population. METHODS: Serum samples from workers in slaughterhouse were collected and tested for the detection of cAg, toxo-IgG, toxo-IgM antibody to toxoplasma, using ELISA. Behavioral measurements were conducted through questionnaire, which was developed according to theory of reasoned action which consists of a measurement model that specifies the relation of measured to behavioral variables and a behavioral variable model to show the influence of behavior variables on each other...
February 2002: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Y Sukthana, T Chintana, W Supatanapong, C Siripan, A Lekkla, R Cheabchalrad
The predictive value of commercial latex agglutination kit (Toxo-Screen DA, bioMerieux) was assessed for use as screening test for Toxoplasma IgG antibody. The sensivity and specificity were also compared with those of the reference standard Sabin-Feldman dye test. Five hundred serum samples were collected from 200 blood donors and 100 each from pregnant women, kidney recipients and HIV infected persons. Eighty (16.0%) out of 500 subjects were positive for Toxoplama IgG antibody by Toxo-Screen DA (bioMerieux) compared with 57 (11...
June 2001: Southeast Asian Journal of Tropical Medicine and Public Health
G Ballarini, P Martelli
On the basis of available literature concerning Toxoplama gondii infection in swine (swine toxoplasmosis) and the resistance of parasite to heating, freezing, gamma-radiation, salting, seasoning and production processes of "cooked" and "fermented" salami, the possible risk of transmission of the disease from pig (where toxoplasmosis is widely diffuse) to man is discussed. No risk of transmission of the disease, even to children and pregnant women, can derive from the consumption of cooked meat, cooked or salted-fermented salami and ham...
2000: Acta Bio-medica de L'Ateneo Parmense: Organo Della Società di Medicina e Scienze Naturali di Parma
A I Baba, O Rotaru
Toxoplasmosis was clinically diagnosed in two dogs of 4 and respectively 18 months. A seven-day treatment remained inefficient and the animals died. Autopsy revealed a global inflammation of the lung, with necrotic lesions of bronchial lymph nodes and acute hyperplastic reaction of the spleen. Histologically, there were identified a diffuse, serofibrinous inflammation of the lung with necrotic foci, fibrinoleucocytic and necrotic exudate of bronchial and portal lymph nodes, small necrotic or fibrinonecrotic foci of the liver and spleen, lymphoglial foci and edema at the base of the cerebellum...
January 1983: Morphologie et Embryologie
F Gray, R Gherardi, E Wingate, J Wingate, G Fénelon, A Gaston, A Sobel, J Poirier
Four patients with AIDS presented with a rapidly fatal global neurological illness. CT did not show any focal lesion and gross post mortem examination of the brain was normal in three of the four cases. Microscopic examination revealed numerous widespread microglial nodules in the brain parenchyma, most containing central toxoplama cysts or free tachyzoites. Such diffuse, non-necrotic, "encephalitic" forms of cerebral toxoplasmosis appear unique to AIDS and, to our knowledge, have not been documented previously...
July 1989: Journal of Neurology
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