keyword
MENU ▼
Read by QxMD icon Read
search

Pseudomallei

keyword
https://www.readbyqxmd.com/read/28729203/epidemiological-cut-off-value-of-clinical-isolates-of-burkholderia-pseudomallei-from-northern-queensland-to-meropenem-ceftazidime-trimethoprim-sulphamethoxazole-and-doxycycline-by-microbroth-dilution
#1
Samuel Maloney, Cathy Engler, Robert Norton
Melioidosis is caused by the bacteria Burkholderia pseudomallei. The most common antibiotics used to treat melioidosis in Australia are meropenem, ceftazidime, trimethoprim-sulphamethoxazole (TMP-SMX), and doxycycline. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) and The Clinical & Laboratory Standards Institute (CLSI) do not provide standards for assessing the susceptibility of B. pseudomallei for these agents. The International Standards Organisation (ISO) microbroth dilution method is accepted by both CLSI and EUCAST as the gold standard of antimicrobial susceptibility testing...
July 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28728591/antimicrobial-susceptibility-pattern-of-clinical-isolates-of-burkholderia-pseudomallei-in-bangladesh
#2
Subarna Dutta, Sabah Haq, Mohammad Rokibul Hasan, Jalaluddin Ashraful Haq
BACKGROUND: Melioidosis an infectious disease, caused by a Gram negative bacterium called Burkholderia pseudomallei, is endemic in Bangladesh. This organism is sensitive to limited number of antimicrobial agents and need prolonged treatment. There is no comprehensive data on the antimicrobial susceptibility profile of B. pseudomallei isolated in Bangladesh over last several years. The present study aimed to determine the antimicrobial susceptibility pattern of B. pseudomallei isolated in a tertiary care hospital of Dhaka city from 2009 to 2015...
July 20, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28719324/susceptibility-of-clinical-isolates-of-burkholderia-pseudomallei-to-a-lipid-a-biosynthesis-inhibitor
#3
Sineenart Sengyee, Natnaree Saiprom, Suporn Paksanont, Direk Limmathurotsakul, Vanaporn Wuthiekanun, Narisara Chantratita
Burkholderia pseudomallei is the causative agent of melioidosis, a serious infection associated with high mortality and relapse. Current antimicrobial therapy using ceftazidime (CAZ) is often ineffective. Inhibitors of LpxC, the enzyme responsible for lipid A biosynthesis, have potential antimicrobial activity against several Gram-negative bacteria in vivo, but their activity against B. pseudomallei is unclear. Herein, we investigated the susceptibility of B. pseudomallei clinical isolates to LpxC-4, an LpxC inhibitor, and LpxC-4 in combination with CAZ...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28719265/differences-in-inflammation-patterns-induced-by-african-and-asian-burkholderia-pseudomallei-isolates-in-mice
#4
Tassili A F Weehuizen, Emma Birnie, Bart Ferwerda, Joris J T H Roelofs, Alex F de Vos, Martin P Grobusch, W Joost Wiersinga
AbstractBurkholderia pseudomallei is the causative agent of melioidosis, an emerging tropical disease of high mortality. Sub-Saharan Africa represents potential melioidosis "hotspots"; however, to date, only a few cases have been reported. Here in, we compared the inflammatory patterns induced by a B. pseudomallei strain recently isolated from a fatal Gabonese case with the Thai reference strain B. pseudomallei-1026b and Burkholderia thailandensis-E264. Ex vivo, no differences were observed in terms of cellular responsiveness between strains...
June 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28707874/designing-probes-for-immunodiagnostics-structural-insights-into-an-epitope-targeting-i-burkholderia-i-infections
#5
Riccardo Capelli, Elena Matterazzo, Marco Amabili, Claudio Peri, Alessandro Gori, Paola Gagni, Marcella Chiari, Ganjana Lertmemongkolchai, Marina Cretich, Martino Bolognesi, Giorgio Colombo, Louise Jane Gourlay
Structure-based epitope prediction drives the design of diagnostic peptidic probes to reveal specific antibodies elicited in response to infections. We previously identified a highly immunoreactive epitope from the peptidoglycan-associated lipoprotein (Pal) antigen from <i>Burkholderia pseudomallei</i>, which could also diagnose <i>Burkholderia cepacia</i> infections. Here, considering the high phylogenetic conservation within <i>Burkholderia</i> species, we ask whether cross-reactivity can be reciprocally displayed by the synthetic epitope from <i>B...
July 14, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28701847/melioidosis-an-emerging-infection-with-fatal-outcomes
#6
Isabella Princess, R Ebenezer, Nagarajan Ramakrishnan, Arun Kumar Daniel, S Nandini, M A Thirunarayan
According to the Centers for Disease Control and Prevention, from being nonendemic for melioidosis, India has now become endemic for the disease since 2012. Until then, melioidosis cases were being reported sporadically from India. There have been isolated case reports from few states across the country for the past few years. Most of the times, Burkholderia pseudomallei may be misreported as Pseudomonas species, especially in resource-poor laboratories. Due to its varied clinical presentation, the specific clinical diagnosis can be difficult, thereby making laboratory diagnosis mandatory...
June 2017: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/28687504/infected-abdominal-aortic-aneurysms-treated-with-extra-anatomic-prosthesis-bypass-in-the-retroperitoneum
#7
You-Fei Qi, Zhan-Xiang Xiao, Chang Shu, Jie Yue, Sa-Hua Liu, Hao Chen, Zhao-Fan Zeng, Wen-Bo Zhang
BACKGROUND: Infected abdominal aortic aneurysms (iAAAs) are rare but life-threatening diseases. The purpose of the present study was to report our experience of extra-anatomic prosthesis bypass in the retroperitoneum as a treatment for iAAAs. METHODS: Data of 8 consecutive patients diagnosed with iAAAs and treated by an extra-anatomic prosthesis bypass in the retroperitoneum were retrospectively collected. Operative details were as follows: one side of the retroperitoneal space was selected to build a track and a bifurcated ePTFE prosthesis was placed through the track...
July 4, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28686630/caspase-6-mediates-resistance-against-burkholderia-pseudomallei-infection-and-influences-the-expression-of-detrimental-cytokines
#8
Alexander Bartel, André Göhler, Verena Hopf, Katrin Breitbach
Caspase-6 is a member of the executioner caspases and known to play a role in innate and adaptive immune processes. However, its role in infectious diseases has rarely been addressed yet. We here examined the impact of caspase-6 in an in vivo infection model using the Gram-negative rod Burkholderia pseudomallei, causing the infectious disease melioidosis that is endemic in tropical and subtropical areas around the world. Caspase-6-/- and C57BL/6 wild type mice were challenged with B. pseudomallei for comparing mortality, bacterial burden and inflammatory cytokine expression...
2017: PloS One
https://www.readbyqxmd.com/read/28673019/gastrointestinal-tract-involvement-in-melioidosis
#9
Prapit Teparrukkul, Worrarat Kongkasame, Songla Chitsaeng, Gumphol Wongsuwan, Vanaporn Wuthiekanun, Sharon J Peacock, Direk Limmathurotsakul
Background: Little is known about the involvement of the human gut in carriage and disease associated with Burkholderia pseudomallei, the cause of melioidosis. Methods: A hospital-based study was conducted in Northeast Thailand to culture stools or rectal swabs from patients with melioidosis, stools from controls with non-infectious diseases, and gastric biopsies from patients undergoing routine endoscopic investigation. Results and Conclusion: B...
April 1, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28665591/biochemical-characterization-and-structural-basis-of-reactivity-and-regioselectivity-differences-between-burkholderia-thailandensis-and-burkholderia-glumae-1-6-didesmethyltoxoflavin-n-methyltransferase
#10
Michael K Fenwick, Khaled H Almabruk, Steven E Ealick, Tadhg P Begley, Benjamin Philmus
Burkholderia glumae converts the guanine base of guanosine triphosphate into an azapteridine and methylates both the pyrimidine and triazine rings to make toxoflavin. Strains of Burkholderia thailandensis and Burkholderia pseudomallei have a gene cluster encoding seven putative biosynthetic enzymes that resembles the toxoflavin gene cluster. Four of the enzymes are similar in sequence to BgToxBCDE, which have been proposed to make 1,6-didesmethyltoxoflavin (1,6-DDMT). One of the remaining enzymes, BthII1283 in B...
July 20, 2017: Biochemistry
https://www.readbyqxmd.com/read/28664152/type-iii-secretion-in-the-melioidosis-pathogen-burkholderia-pseudomallei
#11
REVIEW
Charles W Vander Broek, Joanne M Stevens
Burkholderia pseudomallei is a Gram-negative intracellular pathogen and the causative agent of melioidosis, a severe disease of both humans and animals. Melioidosis is an emerging disease which is predicted to be vastly under-reported. Type III Secretion Systems (T3SSs) are critical virulence factors in Gram negative pathogens of plants and animals. The genome of B. pseudomallei encodes three T3SSs. T3SS-1 and -2, of which little is known, are homologous to Hrp2 secretion systems of the plant pathogens Ralstonia and Xanthomonas...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28658258/genome-scale-analysis-of-the-genes-that-contribute-to-burkholderia-pseudomallei-biofilm-formation-identifies-a-crucial-exopolysaccharide-biosynthesis-gene-cluster
#12
Grace I Borlee, Brooke A Plumley, Kevin H Martin, Nawarat Somprasong, Mihnea R Mangalea, M Nurul Islam, Mary N Burtnick, Paul J Brett, Ivo Steinmetz, David P AuCoin, John T Belisle, Dean C Crick, Herbert P Schweizer, Bradley R Borlee
Burkholderia pseudomallei, the causative agent of melioidosis, is an important public health threat due to limited therapeutic options for treatment. Efforts to improve therapeutics for B. pseudomallei infections are dependent on the need to understand the role of B. pseudomallei biofilm formation and its contribution to antibiotic tolerance and persistence as these are bacterial traits that prevent effective therapy. In order to reveal the genes that regulate and/or contribute to B. pseudomallei 1026b biofilm formation, we screened a sequence defined two-allele transposon library and identified 118 transposon insertion mutants that were deficient in biofilm formation...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28651407/-homology-analysis-and-historical-tracing-for-inter-continental-burkholderia-pseudomallei-strains-of-sequence-type-562
#13
X Zheng, L X Wang, H Wu, H Chen, X Zhu, J R He, L X Xia, W Li
Objective: To understand the homology of sequence type 562 (ST562) strains of Burkholderia pseudomallei which circulated in two separate continents (Asia and Australia) at different times. Methods:SpeⅠrestriction fragments and 4-locus multiple locus variable number tandem repeat analysis (MLVA-4) profiles were extracted from MSHR5858 (ST562 Australia strain) and 350105 (ST562 historical strain of Hainan) genomes respectively by in silico analysis and then compared with the PFGE and MLVA-4 results of five ST562 clinical isolates from Hainan to test their homology...
May 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28649808/development-of-a-real-time-loop-mediated-isothermal-amplification-assay-for-detection-of-burkholderia-mallei
#14
V Pal, A Saxena, S Singh, A K Goel, J S Kumar, M M Parida, G P Rai
Burkholderia mallei is the aetiological agent of glanders, a highly contagious and re-emerging zoonotic disease. Early diagnosis of glanders is critically important to ensure timely treatment with appropriate antibiotics in humans, and to prevent spread of infection in animals. Molecular detection of B. mallei has always been troublesome because of its genetic similarity with Burkholderia pseudomallei, the causative agent of melioidosis. In present investigation, a set of six B. mallei-specific primers were designed and a simple, rapid, specific and sensitive real-time loop-mediated isothermal amplification (LAMP) assay was developed for detection of B...
June 25, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28643413/effects-of-sodium-chloride-on-heat-resistance-oxidative-susceptibility-motility-biofilm-and-plaque-formation-of-burkholderia-pseudomallei
#15
Pornpan Pumirat, Muthita Vanaporn, Usa Boonyuen, Nitaya Indrawattana, Amporn Rungruengkitkun, Narisara Chantratita
Burkholderia pseudomallei is an environmental saprophyte and the causative agent of melioidosis, a severe infectious disease prevalent in tropical areas, including southeast Asia and northern Australia. In Thailand, the highest incidence of melioidosis is in the northeast region, where saline soil and water are abundant. We hypothesized that B. pseudomallei develops an ability to thrive in saline conditions and gains a selective ecological advantage over other soil-dwelling microorganisms. However, little is known about how an elevated NaCl concentration affects survival and adaptive changes in this pathogen...
June 23, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28637908/rapid-filter-based-detection-and-culture-of-burkholderia-pseudomallei-from-small-volumes-of-urine
#16
Pierre A Michel, Christine Lascols, Jay E Gee, Linda M Weigel, David Sue
Clinical outcomes of melioidosis patients improve when the infecting agent, Burkholderia pseudomallei, is rapidly detected and identified by laboratory testing. Detection of B. pseudomallei DNA or recovery of the pathogen by culture from urine can support a diagnosis of melioidosis and guide patient care. Two new methods designated as Filter-Capture DNA Isolation (FCDI) and Filter Cellular Recovery (FCR) were developed to increase the sensitivity of detection and recovery of viable B. pseudomallei cells from small volumes (0...
June 21, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28634144/development-of-a-multiplex-pcr-assay-for-the-detection-and-differentiation-of-burkholderia-pseudomallei-burkholderia-mallei-burkholderia-thailandensis-and-burkholderia-cepacia-complex
#17
Irina Zakharova, Natalya Teteryatnikova, Andrey Toporkov, Dmitry Viktorov
Two species of Burkholderia pseudomallei complex (Bpc), B. pseudomallei and B. mallei, can cause severe life-threatening infections. Rapidly discerning individual species within the group and separating them from other opportunistic pathogens of the Burkholderia cepacia complex (Bcc) is essential to establish a correct diagnosis and for epidemiological surveillance. In this study, a multiplex PCR assay based on the detection of an individual set of chromosomal beta-lactamase genes for single-step identification and differentiation of B...
June 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28628607/host-gene-expression-analysis-in-sri-lankan-melioidosis-patients
#18
Shivankari Krishnananthasivam, Nimanthi Jayathilaka, Harindra Darshana Sathkumara, Enoka Corea, Mohan Natesan, Aruna Dharshan De Silva
BACKGROUND: Melioidosis is a life threatening infectious disease caused by the gram-negative bacillus Burkholderia pseudomallei predominantly found in southeast Asia and northern Australia. Studying the host transcription profiles in response to infection is crucial for understanding disease pathogenesis and correlates of disease severity, which may help improve therapeutic intervention and survival. The aim of this study was to analyze gene expression levels of human host factors in melioidosis patients and establish useful correlation with disease biomarkers, compared to healthy individuals and patients with sepsis caused by other pathogens...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28628442/phylogeography-of-burkholderia-pseudomallei-isolates-western-hemisphere
#19
Jay E Gee, Christopher A Gulvik, Mindy G Elrod, Dhwani Batra, Lori A Rowe, Mili Sheth, Alex R Hoffmaster
The bacterium Burkholderia pseudomallei causes melioidosis, which is mainly associated with tropical areas. We analyzed single-nucleotide polymorphisms (SNPs) among genome sequences from isolates of B. pseudomallei that originated in the Western Hemisphere by comparing them with genome sequences of isolates that originated in the Eastern Hemisphere. Analysis indicated that isolates from the Western Hemisphere form a distinct clade, which supports the hypothesis that these isolates were derived from a constricted seeding event from Africa...
July 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28599008/pediatric-melioidosis-in-sarawak-malaysia-epidemiological-clinical-and-microbiological-characteristics
#20
Anand Mohan, Yuwana Podin, Nickson Tai, Chae-Hee Chieng, Vanessa Rigas, Barbara Machunter, Mark Mayo, Desiree Wong, Su-Lin Chien, Lee-See Tan, Charles Goh, Reginal Bantin, Alexander Mijen, Wen-Yi Chua, King-Ching Hii, See-Chang Wong, Hie-Ung Ngian, Jin-Shyan Wong, Jamilah Hashim, Bart J Currie, Mong-How Ooi
BACKGROUND: Melioidosis is a serious, and potentially fatal community-acquired infection endemic to northern Australia and Southeast Asia, including Sarawak, Malaysia. The disease, caused by the usually intrinsically aminoglycoside-resistant Burkholderia pseudomallei, most commonly affects adults with predisposing risk factors. There are limited data on pediatric melioidosis in Sarawak. METHODS: A part prospective, part retrospective study of children aged <15 years with culture-confirmed melioidosis was conducted in the 3 major public hospitals in Central Sarawak between 2009 and 2014...
June 2017: PLoS Neglected Tropical Diseases
keyword
keyword
15738
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"