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Pseudomallei

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https://www.readbyqxmd.com/read/27934296/identification-of-candidate-host-cell-factors-required-for-actin-based-motility-of-burkholderia-pseudomallei
#1
Niramol Jitprasutwit, Nurhamimah Zainal-Abidin, Charles Vander Broek, Dominic Kurian, Sunee Korbsrisate, Mark P Stevens, Joanne M Stevens
Intracellular actin-based motility of the melioidosis pathogen Burkholderia pseudomallei requires the bacterial factor BimA. Located at one pole of the bacterium, BimA recruits and polymerizes cellular actin to promote bacterial motility within and between cells. Here, we describe an affinity approach coupled with mass spectrometry to identify cellular proteins recruited to BimA-expressing bacteria under conditions that promote actin polymerization. We identified a group of cellular proteins that are recruited to the B...
December 2, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27914949/anion-inhibition-profiles-of-the-%C3%AE-carbonic-anhydrase-from-the-pathogenic-bacterium-burkholderia-pseudomallei-responsible-of-melioidosis-and-highly-drug-resistant-to-common-antibiotics
#2
Sonia Del Prete, Daniela Vullo, Pietro Di Fonzo, Sameh M Osman, Zeid AlOthman, Claudiu T Supuran, Clemente Capasso
Burkholderia pseudomallei is a Gram-negative saprophytic bacterium responsible of melioidosis, an endemic disease of tropical and sub-tropical regions of the world. A recombinant γ-CA (BpsγCA) identified in the genome of this bacterium was cloned and purified. Its catalytic activity and anion inhibition profiles were investigated. The enzyme was an efficient catalyst for the CO2 hydration showing a kcat of 5.3×10(5)s(-1) and kcat/Km of 2.5×10(7)M(-1)×s(-1). The best BpsγCA inhibitors were sulfamide, sulfamic acid, phenylboronic acid and phenylarsonic acid, which showed KI in the range of 49-83μM (these inhibitors showed millimolar inhibition constant against hCA II), followed by diethyldithiocarbamate, selenate, tellurate, perrhenate, selenocyanate, trithiocarbonate, tetraborato, pyrophosphate, stannate, carbonate, bicarbonate, azide, cyanide, thiocyanate and cyanate with KIs in the range of 0...
November 14, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/27898739/environmental-free-living-amoebae-isolated-from-soil-in-khon-kaen-thailand-antagonize-burkholderia-pseudomallei
#3
Parumon Noinarin, Pisit Chareonsudjai, Pinich Wangsomnuk, Surasak Wongratanacheewin, Sorujsiri Chareonsudjai
Presence of Burkholderia pseudomallei in soil and water is correlated with endemicity of melioidosis in Southeast Asia and northern Australia. Several biological and physico-chemical factors have been shown to influence persistence of B. pseudomallei in the environment of endemic areas. This study was the first to evaluate the interaction of B. pseudomallei with soil amoebae isolated from B. pseudomallei-positive soil site in Khon Kaen, Thailand. Four species of amoebae, Paravahlkampfia ustiana, Acanthamoeba sp...
2016: PloS One
https://www.readbyqxmd.com/read/27894256/burkholderia-pseudomallei-derived-mir-3473-enhances-nf-%C3%AE%C2%BAb-via-targeting-traf3-and-is-associated-with-different-inflammatory-responses-compared-to-burkholderia-thailandensis-in-murine-macrophages
#4
Yao Fang, Hai Chen, Yi Hu, Qian Li, Zhiqiang Hu, Tengfei Ma, Xuhu Mao
BACKGROUND: Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a kind of tropical disease. Burkholderia thailandensis (Bt), with a high sequence similarity to Bp, is thought to be an avirulent organism. Since there are numerous similarities between Bp and Bt, their differences in pathogenesis of host response and related mechanism are still undermined. In recent years, microRNAs have been researched in many diseases, but seldom involved in bacterial infection, bacteria-host interaction or explaining the differences between virulent and avirulent species...
November 28, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27881231/burkholderia-pseudomallei-%C3%AE-carbonic-anhydrase-is-strongly-activated-by-amino-acids-and-amines
#5
Daniela Vullo, Sonia Del Prete, Sameh M Osman, Zeid AlOthman, Clemente Capasso, William A Donald, Claudiu T Supuran
Activation of the γ-class carbonic anhydrase (CAs, EC 4.2.1.1) from the pathogenic bacterium Burkholderia pseudomallei (BpsγCA) with a series of natural and non-natural amino acids and aromatic/heterocyclic amines has been investigated. The best BpsγCA activators were d-His, l-DOPA, d-Trp, 4-amino-l-Phe, dopamine, 2-(2-aminoethyl)pyridine, 2-aminoethyl-piparazine/morpholine and l-adrenaline, which showed activation constants ranging between 9 and 86nM. The least effective activators were l-His, l-Phe and 2-pyridyl-methylamine, with KAs in the range of 1...
November 12, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27871233/a-retrospective-analysis-of-melioidosis-in-cambodian-children-2009-2013
#6
Paul Turner, Sabine Kloprogge, Thyl Miliya, Sona Soeng, Pisey Tan, Poda Sar, Pagnarith Yos, Catrin E Moore, Vanaporn Wuthiekanun, Direk Limmathurotsakul, Claudia Turner, Nicholas P J Day, David A B Dance
BACKGROUND: Melioidiosis, infection by Burkholderia pseudomallei, is an important but frequently under-recognised cause of morbidity and mortality in Southeast Asia and elsewhere in the tropics. Data on the epidemiology of paediatric melioidosis in Cambodia are extremely limited. METHODS: Culture-positive melioidosis cases presenting to Angkor Hospital for Children, a non-governmental paediatric hospital located in Siem Reap, Northern Cambodia, between 1(st) January 2009 and 31(st) December 2013 were identified by searches of hospital and laboratory databases and logbooks...
November 21, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27862204/a-comparison-of-the-immunological-potency-of-burkholderia-lipopolysaccharides-in-endotoxemic-balb-c-mice
#7
Pei-Tan Hsueh, Chiu-Lin Liu, Hsuan-Han Wang, Wei-Fen Ni, Ya-Lei Chen, Jong-Kang Liu
Lipopolysaccharide is one of the virulence factors of the soil-borne pathogens Burkholderia pseudomallei, B. thailandensis, B. cenocepacia and B. multivorans, which cause septic melioidosis (often in B. pseudomallei infections but rarely in B. thailandensis infections) or cepacia syndromes (commonly in B. cenocepacia infections but rarely in B. multivorans infections). The inflammatory responses in Burkholderia LPS-induced endotoxemia were evaluated in this study. Prior to induction, the conserved structures and functions of each purified LPS were determined using electrophoretic phenotypes, the ratios of 3-hydroxytetradecanoic to 3-hydroxyhexadecanoic acid and endotoxin units...
November 2016: Microbiology and Immunology
https://www.readbyqxmd.com/read/27843446/pediatric-suppurative-parotitis-caused-by-burkholderia-pseudomallei
#8
Zengzhen Fu, Yingzi Lin, Qiang Wu, Qianfeng Xia
BACKGROUND: Suppurative parotitis caused by Burkholderia pseudomallei has been rarely found outside endemic areas. CASE PRESENTATION: Herein, we report the recovery of Burkholderia pseudomallei from the pus of a suppurative parotitis observed in a 12-year-old boy who lived in Hainan province, China. Specimens of necrotic tissue were collected and sections were stained with hematoxylin and eosin. Pus sample was also collected for bacteriological examination. The suppurative inflammation was observed in the necrotic tissue section and Burkholderia pseudomallei were detected in the sample...
2016: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/27822380/chronic-melioidosis-presenting-with-multiple-abscesses
#9
Anshul Goel, Rahul Bansal, Shweta Sharma, Suman Singhal, Ashok Kumar
Melioidosis is common in Australia and Southeast Asia and is increasingly recognized in India. It presents in various forms which are difficult to identify and often mimics suppurative infections, tuberculosis, fungal infections, malignancy and systemic rheumatic diseases. Presentation may vary from local disease to disseminated abscesses, pneumonia and sepsis. Disease is common and severe in diabetics. We describe a case of diabetic man presenting with fever, septic shock, peri-articular nodules, lung opacities and multiple abscesses in muscles for the past 3 months remaining undiagnosed...
January 2016: Oxford Medical Case Reports
https://www.readbyqxmd.com/read/27812452/efficacy-of-indirect-elisa-for-serodiagnosis-of-melioidosis-using-immunodominant-antigens-from-non-pathogenic-burkholderia-thailandensis
#10
Sumet Wajanarogana, Kanyanan Kritsiriwuthinan
Melioidosis caused by gram negative bacteria, B. pseudomallei, is a fatal disease in the tropical and sub-tropical regions. However, sporadic cases have been reported in elsewhere. Early detection is imperative to reduce the mortality rate. Serological tests have being substantially developed using recombinant proteins as specific targeted antigens to melioidosis antibodies. In the present study, we focus on a truncated flagellin fragment (FLAG300) and outer membrane protein A (OmpABT) of B. thailandensis E264 as potential antigens for developing indirect ELISA to improve the serodiagnosis of melioidosis...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27812400/antimicrobial-activity-of-tachyplesin-1-against-burkholderia-pseudomallei-an-in-vitro-and-in-silico-approach
#11
Lyn-Fay Lee, Vanitha Mariappan, Kumutha Malar Vellasamy, Vannajan Sanghiran Lee, Jamuna Vadivelu
Burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to many conventional antibiotics. Therefore, alternative antimicrobial agents such as antimicrobial peptides (AMPs) are extensively studied to combat this issue. Our study aims to identify and understand the mode of action of the potential AMP(s) that are effective against B. pseudomallei in both planktonic and biofilm state as well as to predict the possible binding targets on using in vitro and in silico approaches. In the in vitro study, 11 AMPs were tested against 100 B...
2016: PeerJ
https://www.readbyqxmd.com/read/27799332/ph-alkalinization-by-chloroquine-suppresses-pathogenic-burkholderia-t6ss-1-and-multi-nucleated-giant-cells
#12
Jennifer Chua, Jeffrey L Senft, Stephen J Lockett, Paul J Brett, Mary N Burtnick, David DeShazer, Arthur M Friedlander
Burkholderia mallei and B. pseudomallei cause glanders and melioidosis, respectively, in humans and animals. A hallmark of pathogenesis is formation of granulomas containing multi-nucleated giant cells (MNGCs) and cell death. These processes depend on the Type 6 secretion system-1 (T6SS-1), which is required for virulence in animals. We examined the cell biology of MNGC formation and cell death. We found that chloroquine (CLQ), an anti-malarial drug, inhibits Burkholderia growth, phagosomal escape and subsequent MNGC formation...
October 31, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27795637/septicemic-melioidosis-in-a-transplant-recipient-causing-graft-dysfunction
#13
S Sathiavageesan
Melioidosis, an infectious disease caused by Burkholderia pseudomallei, is endemic in the Northern Australia and South-East Asia. It is an emerging disease in the Indian subcontinent. Melioidosis tends to run a potentially lethal course in immunocompromised individuals and data in renal transplantation are scarce. The clinical presentation of melioidosis is diverse, mimicking several other infectious diseases. Diagnosis could be delayed in transplant recipients. Choice and duration of antimicrobial therapy, management of immunosuppression, and patient and graft outcomes are other issues to be addressed...
September 2016: Indian Journal of Nephrology
https://www.readbyqxmd.com/read/27792775/immune-modulation-as-an-effective-adjunct-post-exposure-therapeutic-for-b-pseudomallei
#14
William J Wilson, Maryam F Afzali, Jason E Cummings, Marie E Legare, Ronald B Tjalkens, Christopher P Allen, Richard A Slayden, William H Hanneman
Melioidosis is caused by the facultative intracellular bacterium Burkholderia pseudomallei and is potentially fatal. Despite a growing global burden and high fatality rate, little is known about the disease. Recent studies demonstrate that cyclooxygenase-2 (COX-2) inhibition is an effective post-exposure therapeutic for pulmonary melioidosis, which works by inhibiting the production of prostaglandin E2 (PGE2). This treatment, while effective, was conducted using an experimental COX-2 inhibitor that is not approved for human or animal use...
October 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27790560/cutaneous-melioidosis
#15
Basavaprabhu Achappa, Deepak Madi, K Vidyalakshmi
Melioidosis is an emerging infection in India. It usually presents as pneumonia. Melioidosis presenting as cutaneous lesions is uncommon. We present a case of cutaneous melioidosis from Southern India. Cutaneous melioidosis can present as an ulcer, pustule or as crusted erythematous lesions. A 22-year-old gentleman known case of diabetes mellitus was admitted in our hospital with an ulcer over the left thigh. Discharge from the ulcer grew Burkholderia pseudomallei. He was successfully treated with ceftazidime...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27789057/a-case-control-study-of-community-acquired-acinetobacter-baumannii-pneumonia-and-melioidosis-pneumonia-in-northeast-thailand-an-emerging-fatal-disease-with-unique-clinical-features
#16
Samadhi Patamatamkul, Voravan Klungboonkrong, Pakawas Praisarnti, Kittitouch Jirakiat
Acinetobacter baumannii is the emerging cause of severe and often fatal gram-negative, community-acquired pneumonia (CAP-AB) in Thailand. Due to its rarity, its specific clinical features are ill defined. In this retrospective study, we compared the demographic data, risk factors, clinical characteristics, radiographic pattern, and microbiological data between CAP-AB and Burkholderia pseudomallei CAP (CAP-BP) to identify the clinical features and risk factors of CAP-AB. CAP-AB was associated with a more productive cough and a shorter duration of symptoms, while CAP-BP was associated with more musculoskeletal symptoms...
January 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27784725/characterisation-of-homologous-sphingosine-1-phosphate-lyase-s1pl-isoforms-in-the-bacterial-pathogen-burkholderia-pseudomallei
#17
Christopher McLean, Jon Marles-Wright, Rafael Custodio, Jonathan Lowther, Amanda J Kennedy, Jacob Pollock, David J Clarke, Alan R Brown, Dominic J Campopiano
Sphingolipids (SLs) are ubiquitous elements in eukaryotic membranes and are also found in some bacterial and viral species. As well as playing an integral structural role, SLs also act as potent signalling molecules involved in numerous cellular pathways and have been linked to many human diseases. A central SL signalling molecule is sphingosine-1-phosphate (S1P) whose breakdown is catalysed by sphingosine-1-phosphate lyase (S1PL), a pyridoxal 5 '-phosphate (PLP) dependent enzyme that catalyses the cleavage of S1P to (2E)-hexadecenal (2E-HEX) and phosphoethanolamine (PE)...
October 26, 2016: Journal of Lipid Research
https://www.readbyqxmd.com/read/27766174/comparison-of-five-commercial-nucleic-acid-extraction-kits-for-the-pcr-based-detection-of-burkholderia-pseudomallei-dna-in-formalin-fixed-paraffin-embedded-tissues
#18
Sonja Obersteller, Heinrich Neubauer, Ralf Matthias Hagen, Hagen Frickmann
The extraction and further processing of nucleic acids (NA) from formalin-fixed paraffin-embedded (FFPE) tissues for microbiological diagnostic polymerase chain reaction (PCR) approaches is challenging. Here, we assessed the effects of five different commercially available nucleic acid extraction kits on the results of real-time PCR. FFPE samples from organs of Burkholderia pseudomallei-infected Swiss mice were subjected to processing with five different extraction kits from QIAGEN (FFPE DNA Tissue Kit, EZ1 DNA Tissue Kit, DNA Mini Kit, DNA Blood Mini Kit, and FlexiGene DNA Kit) in combination with three different real-time PCRs targeting B...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27763813/trojan-horse-l-selectin-monocytes-a-portal-of-burkholderia-pseudomallei-entry-into-the-brain
#19
James A St John
No abstract text is available yet for this article.
October 20, 2016: Virulence
https://www.readbyqxmd.com/read/27738703/characterization-of-in-vitro-phenotypes-of-burkholderia-pseudomallei-and-burkholderia-mallei-strains-potentially-associated-with-persistent-infection-in-mice
#20
R C Bernhards, C K Cote, K Amemiya, D M Waag, C P Klimko, P L Worsham, S L Welkos
Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), the agents of melioidosis and glanders, respectively, are Tier 1 biothreats. They infect humans and animals, causing disease ranging from acute and fatal to protracted and chronic. Chronic infections are especially challenging to treat, and the identification of in vitro phenotypic markers which signal progression from acute to persistent infection would be extremely valuable. First, a phenotyping strategy was developed employing colony morphotyping, chemical sensitivity testing, macrophage infection, and lipopolysaccharide fingerprint analyses to distinguish Burkholderia strains...
October 13, 2016: Archives of Microbiology
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