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Pseudomallei

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https://www.readbyqxmd.com/read/29329289/burkholderia-pseudomallei-modulates-host-iron-homeostasis-to-facilitate-iron-availability-and-intracellular-survival
#1
Imke H E Schmidt, Claudia Gildhorn, Martha A L Böning, Vera A Kulow, Ivo Steinmetz, Antje Bast
BACKGROUND: The control over iron homeostasis is critical in host-pathogen-interaction. Iron plays not only multiple roles for bacterial growth and pathogenicity, but also for modulation of innate immune responses. Hepcidin is a key regulator of host iron metabolism triggering degradation of the iron exporter ferroportin. Although iron overload in humans is known to increase susceptibility to Burkholderia pseudomallei, it is unclear how the pathogen competes with the host for the metal during infection...
January 12, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29320408/outer-membrane-vesicle-vaccines-from-biosafe-surrogates-prevent-acute-lethal-glanders-in-mice
#2
Michael H Norris, Mohammad S R Khan, Sunisa Chirakul, Herbert P Schweizer, Apichai Tuanyok
Burkholderia mallei is a host-adapted Gram-negative mammalian pathogen that causes the severe disease glanders. Glanders can manifest as a rapid acute progression or a chronic debilitating syndrome primarily affecting solipeds and humans in close association with infected animals. In USA, B. mallei is classified as one of the most important bacterial biothreat agents. Presently, there is no licensed glanders vaccine available for humans or animals. In this work, outer membrane vesicles (OMVs) were isolated from three attenuated biosafe bacterial strains, Burkholderia pseudomallei Bp82, B...
January 10, 2018: Vaccines
https://www.readbyqxmd.com/read/29315931/histopathological-and-immunohistochemical-characterization-of-burkholderia-pseudomallei-lesions-in-an-acute-model-of-infection-with-balb-c-mice
#3
Waldo Luis García-Jiménez, Francisco J Salguero, Riccardo V D'Elia
Organ tissue damage is a key contributor to host morbidity and mortality following infection with microbial agents. Severe immune responses, excessive cellular recruitment and necrosis of cells all play a role in disease pathology. Understanding the pathogenesis of disease can aid in identifying potential new therapeutic targets or simply act as a diagnostic tool. Burkholderia pseudomallei is a Gram-negative bacterium that can cause acute and chronic diseases. The BALB/c mouse has been shown to be highly susceptible to aerosol challenge with B...
January 7, 2018: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/29313474/clinical-bacteriologic-and-geographic-stratification-of-melioidosis-emerges-from-the-sri-lankan-national-surveillance-program
#4
Harindra D Sathkumara, Adam J Merritt, Enoka M Corea, Shivankari Krishnananthasivam, Mohan Natesan, Timothy J J Inglis, Aruna Dharshan De Silva
Melioidosis, a potentially fatal tropical infection, is said to be underdiagnosed in low-income countries. An increase in melioidosis cases in Sri Lanka allowed us to analyze the relationship among clinical outcome, bacteriology, epidemiology, and geography in the first 108 laboratory-confirmed cases of melioidosis from a nationwide surveillance program. The additional 76 cases of laboratory-confirmed melioidosis confirmed additional associations between Burkholderia pseudomallei multilocus sequence typing (MLST) and infection phenotype; ST1137/unifocal bacteremic infection (χ2 = 3...
January 8, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29286449/the-madagascar-hissing-cockroach-as-an-alternative-non-mammalian-animal-model-to-investigate-virulence-pathogenesis-and-drug-efficacy
#5
Jennifer Chua, Nathan A Fisher, Shane D Falcinelli, David DeShazer, Arthur M Friedlander
Many aspects of innate immunity are conserved between mammals and insects. An insect, the Madagascar hissing cockroach from the genus Gromphadorhina, can be utilized as an alternative animal model for the study of virulence, host-pathogen interaction, innate immune response, and drug efficacy. Details for the rearing, care and breeding of the hissing cockroach are provided. We also illustrate how it can be infected with bacteria such as the intracellular pathogens Burkholderia mallei, B. pseudomallei, and B...
November 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29273310/evaluation-of-fimc-and-bdha-based-duplex-pcr-for-specific-identification-and-differentiation-of-burkholderia-pseudomallei-from-near-neighbor-burkholderia-species
#6
Bhavani V Peddayelachagiri, Soumya Paul, Madhurjya Gogoi, Murali H Sripathy, Harsh V Batra
Assays for the rapid detection and accurate differentiation of Burkholderia pseudomallei from near-neighbor species are urgently needed in melioidosis endemic regions due to the high associated mortality and biowarfare importance of the pathogen. PCR-based methods have revolutionized this field due to the accuracy, sensitivity, and specificity that are achievable in a rapid way. In this study, a compound molecular detection system, consisting of a duplex PCR assay, was developed for the specific identification of Burkholderia pseudomallei and differentiation from other Burkholderia species...
November 28, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29260679/melioidosis-singapore-2003-2014
#7
Long Pang, Patrick N A Harris, Rachel L Seiler, Peng Lim Ooi, Jeffrey Cutter, Kee Tai Goh, Alex R Cook, Dale Fisher, Louis Yi Ann Chai
In contrast with northern Australia and Thailand, in Singapore the incidence of melioidosis and co-incidence of melioidosis and pneumonia have declined. Burkholderia pseudomallei deep abscesses increased 20.4% during 2003-2014. These trends could not be explained by the environmental and climatic factors conventionally ascribed to melioidosis.
January 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29242769/vaccines-for-the-prevention-of-melioidosis-and-glanders
#8
Monica M Johnson, Kristy M Ainslie
Purpose of review: Burkholderia pseudomallei's and Burkholderia mallei's high rate of infectivity, limited treatment options, and potential use as biological warfare agents underscore the need for development of effective vaccines against these bacteria. Research efforts focused on vaccines against these bacteria are in pre-clinical stages, with no approved formulations currently on the market. Recent findings: Several live attenuated and subunit vaccine formulations have been evaluated in animal studies, with no reports of significant long term survival after lethal challenge...
September 2017: Current Tropical Medicine Reports
https://www.readbyqxmd.com/read/29240770/emergence-of-melioidosis-in-the-indian-ocean-region-two-new-cases-and-a-literature-review
#9
Nicolas Allou, Olivier Martinet, Jérôme Allyn, Bruno Bouchet, Marie-Christine Jaffar-Bandjee, Thomas Galas, Nicolas Traversier, Olivier Belmonte
Melioidosis is a disease caused by bacteria called B. pseudomallei. Infections can develop after contact with standing water. This disease can reach all the organs and especially the lungs. It is associated with a high mortality rate (up to 50%). Melioidosis is endemic in northern Australia and in Southeast Asia. Nevertheless, B. pseudomallei may be endemic in the Indian Ocean region and in Madagascar in particular, so clinicians and microbiologists should consider acute melioidosis as a differential diagnosis in the Indian Ocean region, in particular from Madagascar...
December 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29238621/burkholderia-aortic-aneurysm-a-case-report-and-review-of-the-literature
#10
Sreelakshmi Panginikkod, Aishwarya Ramachandran, Pratyusha Bollimunta, Roshanak Habibi, Roshan Kumar Arjal, Venu Gopalakrishnan
Melioidosis is a frequently fatal infection caused by the Gram-negative bacillus Burkholderia pseudomallei endemic to Southeast Asia and Northern Australia. It is a rare imported pathogen in the United States and is a potential bioterror agent. We report the case of an 82-year-old previously healthy man who presented with 2 weeks of fever and epigastric pain after he returned from the Philippines. A diagnosis of nondissecting mycotic aneurysm in the descending thoracic aorta was made with the help of CT angiogram and positive blood cultures...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/29232837/a-burkholderia-pseudomallei-outer-membrane-vesicle-vaccine-provides-cross-protection-against-inhalational-glanders-in-mice-and-non-human-primates
#11
Sarah M Baker, Christopher J H Davitt, Natalya Motyka, Nicole L Kikendall, Kasi Russell-Lodrigue, Chad J Roy, Lisa A Morici
Burkholderia mallei is a Gram-negative, non-motile, facultative intracellular bacillus and the causative agent of glanders, a highly contagious zoonotic disease. B. mallei is naturally resistant to multiple antibiotics and there is concern for its potential use as a bioweapon, making the development of a vaccine against B. mallei of critical importance. We have previously demonstrated that immunization with multivalent outer membrane vesicles (OMV) derived from B. pseudomallei provide significant protection against pneumonic melioidosis...
December 9, 2017: Vaccines
https://www.readbyqxmd.com/read/29215024/identification-of-srna-mediated-responses-to-nutrient-depletion-in-burkholderia-pseudomallei
#12
Hirzahida Mohd-Padil, Nadzirah Damiri, Suhaila Sulaiman, Shiao-Fei Chai, Sheila Nathan, Mohd Firdaus-Raih
The Burkholderia genus includes many species that are known to survive in diverse environmental conditions including low nutrient environments. One species, Burkholderia pseudomallei is a versatile pathogen that can survive in a wide range of hosts and environmental conditions. In this study, we investigated how a nutrient depleted growth environment evokes sRNA mediated responses by B. pseudomallei. Computationally predicted B. pseudomallei D286 sRNAs were mapped to RNA-sequencing data for cultures grown under two conditions: (1) BHIB as a nutrient rich media reference environment and (2) M9 media as a nutrient depleted stress environment...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29212292/construction-of-luminescence-and-fluorescence-tagged-burkholderia-pseudomallei-for-pathogen-tracking-in-a-mouse-model
#13
Yong-Woo Shin, Deok Bum Park, Myung-Min Choi, Jeong-Hoon Chun, Baik-Lin Seong, Gi-Eun Rhie
Molecular imaging is a powerful method for tracking various infectious disease causing pathogens in host organisms. Currently, a dual molecular imaging method which can provide temporal and spatial information in infected hosts at organism, organ, tissue and cellular levels simultaneously has not been reported for Burkholderia pseudomallei, a high-risk pathogen that causes melioidosis. In this study, we have established an experiment method that provides spatio-temporal information in infected hosts using luminescent and fluorescent dual-labeled B...
December 8, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29208745/the-complex-quorum-sensing-circuitry-of-burkholderia-thailandensis-is-both-hierarchically-and-homeostatically-organized
#14
Servane Le Guillouzer, Marie-Christine Groleau, Eric Déziel
The genome of the bacterium Burkholderia thailandensis encodes three complete LuxI/LuxR-type quorum sensing (QS) systems: BtaI1/BtaR1 (QS-1), BtaI2/BtaR2 (QS-2), and BtaI3/BtaR3 (QS-3). The LuxR-type transcriptional regulators BtaR1, BtaR2, and BtaR3 modulate the expression of target genes in association with various N-acyl-l-homoserine lactones (AHLs) as signaling molecules produced by the LuxI-type synthases BtaI1, BtaI2, and BtaI3. We have systematically dissected the complex QS circuitry of B. thailandensis strain E264...
December 5, 2017: MBio
https://www.readbyqxmd.com/read/29208140/suspected-cases-of-intracontinental-burkholderia-pseudomallei-sequence-type-homoplasy-resolved-using-whole-genome-sequencing
#15
Ammar Aziz, Derek S Sarovich, Tegan M Harris, Mirjam Kaestli, Evan McRobb, Mark Mayo, Bart J Currie, Erin P Price
Burkholderia pseudomallei is a Gram-negative environmental bacterium that causes melioidosis, a disease of high mortality in humans and animals. Multilocus sequence typing (MLST) is a popular and portable genotyping method that has been used extensively to characterise the genetic diversity of B. pseudomallei populations. MLST has been central to our understanding of the underlying phylogeographical signal present in the B. pseudomallei genome, revealing distinct populations on both the intra- and the inter-continental level...
November 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29208119/comparative-analysis-of-the-burkholderia-cenocepacia-k56-2-essential-genome-reveals-cell-envelope-functions-that-are-uniquely-required-for-survival-in-species-of-the-genus-burkholderia
#16
April S Gislason, Keith Turner, Mike Domaratzki, Silvia T Cardona
Burkholderia cenocepacia K56-2 belongs to the Burkholderia cepacia complex, a group of Gram-negative opportunistic pathogens that have large and dynamic genomes. In this work, we identified the essential genome of B. cenocepacia K56-2 using high-density transposon mutagenesis and insertion site sequencing (Tn-seq circle). We constructed a library of one million transposon mutants and identified the transposon insertions at an average of one insertion per 27 bp. The probability of gene essentiality was determined by comparing of the insertion density per gene with the variance of neutral datasets generated by Monte Carlo simulations...
November 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29188170/the-role-of-climate-in-the-epidemiology-of-melioidosis
#17
REVIEW
Adam J Merritt, Timothy J J Inglis
Purpose of Review: Melioidosis epidemiology is susceptible to climate change through direct and indirect effects on human encounter with the causative agent, Burkholderia pseudomallei. This review describes the current depth of knowledge and recent advances in the understanding of this relationship and applies it to observations of melioidosis in Western Australia. Recent Findings: High maximum rainfall and dense cloud cover have been shown to predict environmental presence of B...
2017: Current Tropical Medicine Reports
https://www.readbyqxmd.com/read/29187254/acute-disseminated-melioidosis-giving-rise-to-pneumonia-and-renal-abscesses-complicated-with-thrombotic-thrombocytopenic-purpura-in-a-post-partum-woman-a-case-report
#18
Piyumi Sachindra Alwis Wijewickrama, Rohini Weerakoon
BACKGROUND: Melioidosis is an established endemic infection in Sri Lanka, caused by Burkholderia pseudomallei, a gram negative bacterium distributed in saprophytes in soil and surface water. Main mode of transmission is via percutaneous inoculation. Pneumonia is the most common presentation in acute disease. CASE PRESENTATION: We report a 33 year old previously healthy Sinhalese female with an occupational exposure to surface water in paddy fields, who was on postpartum day 6 following an uncomplicated pregnancy and delivery via an elective caesarian section...
November 29, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29178614/antibacterial-activity-of-chitosan-against-burkholderia-pseudomallei
#19
Watcharaporn Kamjumphol, Pisit Chareonsudjai, Sorujsiri Chareonsudjai
The ability of Burkholderia pseudomallei to persist and survive in the environment is a health problem worldwide. Therefore, the antibacterial activities of chitosan against four environmental isolates of B. pseudomallei from soil in Khon Kaen, Thailand, were investigated. Antibacterial activities were assessed by a plate count technique after treatment with 0.2, 0.5, 1, 2 or 5 mg ml-1 chitosan for 0, 24 and 48 hr. Chitosan at 5 mg ml-1 completely killed all four B. pseudomallei isolates within 24 hr, whilst 2 mg ml-1 chitosan lowered the viability of B...
November 27, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/29177837/high-throughput-flow-cytometry-screening-of-multidrug-efflux-systems
#20
Mark K Haynes, Matthew Garcia, Ryan Peters, Anna Waller, Pietro Tedesco, Oleg Ursu, Cristian G Bologa, Radleigh G Santos, Clemencia Pinilla, Terry H Wu, Julie A Lovchik, Tudor I Oprea, Larry A Sklar, George P Tegos
The resistance nodulation cell division (RND) family of proteins are inner membrane transporters that associate with periplasmic adaptor proteins and outer membrane porins to affect substrate transport from the cytosol and periplasm in Gram-negative bacteria. Various structurally diverse compounds are substrates of RND transporters. Along with their notable role in antibiotic resistance, these transporters are essential for niche colonization, quorum sensing, and virulence as well as for the removal of fatty acids and bile salts...
2018: Methods in Molecular Biology
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