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https://www.readbyqxmd.com/read/27909219/superior-survival-of-ex-vivo-cultured-human-reticulocytes-following-transfusion-into-mice
#1
Sabine Kupzig, Stephen F Parsons, Elinor Curnow, David J Anstee, Allison Blair
The generation of cultured red blood cells from stem cell sources may fill an unmet clinical need for transfusion dependent patients, particularly in countries that lack a sufficient and safe blood supply. Cultured red blood cells were generated from human CD34+ cells from adult peripheral blood or cord blood by ex vivo expansion and a comprehensive in vivo survival comparison with standard red cell concentrates was undertaken. Significant amplification (>105 fold) was achieved using CD34+ cells from both cord blood and peripheral blood, generating high yields of enucleated cultured red blood cells...
December 1, 2016: Haematologica
https://www.readbyqxmd.com/read/27905002/disseminated-candidiasis-in-a-young-previously-healthy-dog-and-review-of-literature
#2
Nicole Willems, Dirk J Houwers, Yvette M Schlotter, Bart Theelen, Teun Boekhout
BACKGROUND: The reports on disseminated candidiasis in dogs so far describe at least one predisposing factor. This case report, however, highlights candidiasis in a dog without any known predisposition. PATIENT: A 1.5-year-old intact female Hovawart dog was presented with subcutaneous nodules and polyuria/polydipsia. An excisional biopsy revealed a chronic pyogranulomatous and necrotizing inflammation with mycotic structures. The patient became febrile and lethargic, and developed lameness...
November 30, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27902388/the-exploration-of-risk-factors-of-concurrent-bacteremia-among-critically-ill-patients-with-severe-dengue
#3
Chin-Ming Chen, Khee-Siang Chan, Kuo-Chen Cheng, Willy Chou, Hui-Chun Chao, Chiu-Yin Yeh, Wen-Liang Yu
We investigated the clinical features of intensive care unit (ICU) patients with a concomitant severe dengue infection and bacteremia to identify risk factors for this comorbidity. The records of all ICU dengue patients admitted during a July 31-November 31, 2015 were reviewed. Patients with "concurrent bacteremia" (positive bacterial blood culture within 72 hours of ICU admission) were identified. ICU admission was required for 142 patients; 22 (15.5%) had concurrent bacteremia. Streptococcus spp. was the most common pathogen, then Escherichia coli, and then Staphylococcus spp...
November 7, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27900629/dracorhodin-perochlorate-attenuates-staphylococcus-aureus-usa300-virulence-by-decreasing-%C3%AE-toxin-expression
#4
Yumin Liu, Dongxue Shi, Yan Guo, Meng Li, Yonghong Zha, Quankai Wang, Jianfeng Wang
α-Toxin, a pore-forming toxin secreted by most Staphylococcus aureus, plays critical role in the pathogenesis associated with various infectious diseases. The USA300 which is a major international epidemic methicilin-resisrant S. aureus has spread rapidly to multiple countries and become an emerging public health concern. In this study, the in vitro efficacy of Dracorhodin Perochlorate (DP) against USA300 virulence was evaluated. Using susceptibility testing, immunoblots, rabbit blood haemolytic assay and real-time RT-PCR, we observed that the α-toxin production was decreased when USA300 was co-cultured with different sub-inhibitory concentration of DP...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27900338/the-bridge-enhanced-anterior-cruciate-ligament-repair-bear-procedure-an-early-feasibility-cohort-study
#5
Martha M Murray, Brett M Flutie, Leslie A Kalish, Kirsten Ecklund, Braden C Fleming, Benedikt L Proffen, Lyle J Micheli
BACKGROUND: This study assessed the safety of the newly developed bridge-enhanced anterior cruciate ligament (ACL) repair (BEAR), which involves suture repair of the ligament combined with a bioactive scaffold to bridge the gap between the torn ligament ends. As the intra-articular environment is complex in its response to implanted materials, this study was designed to determine whether there would be a significant rate of adverse reaction to the implanted scaffold. HYPOTHESIS: The primary hypothesis was that the implanted scaffold would not result in a deep joint infection (arthrocentesis with positive culture) or significant inflammation (clinical symptoms justifying arthrocentesis but negative culture)...
November 2016: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/27899144/hypoxia-induced-responses-by-endothelial-colony-forming-cells-are-modulated-by-placental-growth-factor
#6
Michelle B Hookham, Imran H A Ali, Christina L O'Neill, Emer Hackett, Melanie H Lambe, Tina Schmidt, Reinhold J Medina, Sara Chamney, Bharathi Rao, Eibhlin McLoone, David Sweet, Alan W Stitt, Derek P Brazil
BACKGROUND: Endothelial colony-forming cells (ECFCs), also termed late outgrowth endothelial cells, are a well-defined circulating endothelial progenitor cell type with an established role in vascular repair. ECFCs have clear potential for cell therapy to treat ischaemic disease, although the precise mechanism(s) underlying their response to hypoxia remains ill-defined. METHODS: In this study, we isolated ECFCs from umbilical cord blood and cultured them on collagen...
November 29, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27898898/effects-of-molasses-and-crude-glycerol-combined-in-a-liquid-supplement-on-ruminal-fermentation-in-beef-steers-consuming-bermudagrass-hay
#7
F M Ciriaco, D D Henry, V R G Mercadante, T M Schulmeister, M Ruiz-Moreno, G C Lamb, N DiLorenzo
Two experiments were performed to evaluate the effects of 1) increasing supplementation doses of a 50:50 (as-fed) liquid supplement of molasses and crude glycerol (M:G) on ruminal fermentation parameters and blood urea nitrogen (BUN) in beef steers consuming Tifton 85 bermudagrass ( spp.) hay and 2) different proportions of molasses and crude glycerol in a liquid supplement on in vitro fermentation and gas production kinetics. For Exp. 1, 8 ruminally cannulated, Angus-crossbred steers were used in a duplicated 4 × 4 Latin square design, had ad libitum access to Tifton 85 bermudagrass hay, and were randomly assigned to 1 of 4 treatments: 1) CTRL, no supplementation; 2) SUP1, 0...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898871/effect-of-disodium-calcium-malate-or-supplementation-on-growth-performance-carcass-quality-ruminal-fermentation-products-and-blood-metabolites-of-heifers
#8
C Carrasco, P Medel, A Fuentetaja, M J Ranilla, M D Carro
The aim of this study was to assess the effects of malate salts and culture on growth performance, carcass quality, ruminal fermentation products, and blood metabolites in heifers raised under southern Europe practical farm conditions. A total of 108 Charolaise cross heifers (214 ± 27.3 kg BW and 6.4 ± 1.1 mo of age) were housed in 18 pens of 6 animals each and used in a 114-d feedlot study. There was a totally randomized experimental design, and 6 pens were assigned to each of the following experimental diets: a control (no supplementation), the control plus 4 g of disodium/calcium malate mixture per kilogram of concentrate (2...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27897434/time-to-positivity-type-of-culture-media-and-oxidase-test-performed-on-positive-blood-culture-vials-to-predict-pseudomonas-aeruginosa-in-patients-with-gram-negative-bacilli-bacteraemia
#9
N Cobos-Triguero, Y Zboromyrska, L Morata, I Alejo, C De La Calle, A Vergara, C Cardozo, M P Arcas, A Soriano, F Marco, J Mensa, M Almela, J A Martínez
OBJECTIVE: The aim of this study was to determine the usefulness of oxidase test and time-to-positivity (TTP) in aerobic and anaerobic blood culture vials to detect the presence of Pseudomonas aeruginosa in patients with Gram-negative bacilli (GNB) bacteraemia. METHODS: TTP was recorded for each aerobic and anaerobic blood culture vial of monomicrobial bacteraemia due to GNB. Oxidase test was performed in a pellet of the centrifuged content of the positive blood culture...
November 25, 2016: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/27897049/using-text-messages-to-promote-health-in-african-americans-hearthealthyandcancerfree
#10
Allison R Jones, Debra K Moser, Jennifer Hatcher
OBJECTIVES: African-Americans are vulnerable to both cancer and cardiovascular disease (CVD) due to intricately connected risk factors. Use of text messages is an innovative method to provide health information to reduce these risks. The aim of this study was to test the feasibility and acceptability of a text messaging intervention to reduce CVD and cancer risk factors in African-Americans. DESIGN: We developed an intervention using text messages culturally tailored for African-Americans over age 50 who were at risk (one or more modifiable risk factors) for CVD and/or cancer...
November 29, 2016: Ethnicity & Health
https://www.readbyqxmd.com/read/27896194/osteomyelitis-following-domestic-animal-bites-to-the-hand-two-case-reports-and-practical-guidelines
#11
Jung Soo Lim, Jin Hwan Byun, Kyung Hee Min, Hye Kyung Lee, Yun Sun Choi
Recently, the number of cases of animal bite wounds has increased significantly in concordance with an increase in the pet population around the world. The authors report two rare cases of osteomyelitis of the phalanx following cat and dog bites. On initial physical examination, signs of a severe infection were observed. Radiographs of both patients showed the presence of osteomyelitis, and in one of the patients, the diagnosis was confirmed with a bone biopsy. After use of empirical antibiotics, intravenous antibiotic therapy that matched the identified bacterium's sensitivity was initiated, and at the same time, secure dressing including debridement was performed to induce secondary healing...
November 2016: Archives of Plastic Surgery
https://www.readbyqxmd.com/read/27895516/a-prospective-observational-study-comparing-the-pk-pd-relationships-of-generic-meropenem-mercide-%C3%A2-to-the-innovator-brand-in-critically-ill-patients
#12
Mervyn Mer, Jacques Rene Snyman, Constance Elizabeth Jansen van Rensburg, Jacob John van Tonder, Ilze Laurens
INTRODUCTION: Clinicians' skepticism, fueled by evidence of inferiority of some multisource generic antimicrobial products, results in the underutilization of more cost-effective generics, especially in critically ill patients. The aim of this observational study was to demonstrate equivalence between the generic or comparator brand of meropenem (Mercide(®)) and the leading innovator brand (Meronem(®)) by means of an ex vivo technique whereby antimicrobial activity is used to estimate plasma concentration of the active moiety...
2016: Clinical Pharmacology: Advances and Applications
https://www.readbyqxmd.com/read/27893534/clinical-predictors-of-positive-postoperative-blood-cultures
#13
Libby R Copeland-Halperin, Joshua Stodghill, Erica Emery, Amber W Trickey, Jonathan Dort
OBJECTIVE: To define clinical features of surgical patients in whom postoperative blood cultures are likely to identify pathogens. BACKGROUND: Bacteremia is a worrisome postoperative complication and blood cultures (BCx) are routinely used for evaluation of postoperative bacteremia, but are costly and not always diagnostic. Better methods are needed to select patients in whom BCx identify pathogens. METHODS: We reviewed records of patients ≥18 years old with BCx drawn ≤10 days after surgery in 2013 seeking independent predictors of positive cultures by simple and multiple logistic regression models with statistical significance at α = 0...
November 23, 2016: Annals of Surgery
https://www.readbyqxmd.com/read/27892870/nitric-oxide-modulates-the-immunological-response-of-bovine-pbmcs-in-an-in-vitro-brdc-infection-model
#14
Michael Peter Sheridan, Gilly Regev-Shoshani, James Martins, Selvarani Vimalanathan, Chris Miller
Bovine respiratory disease complex (BRDc) is a multi-factorial disease, involving both viral and bacterial pathogens, that negatively impacts the cattle feedlot industry. A nitric oxide releasing solution (NORS) has been developed and shown to have potential in the prevention of BRDc. This study investigated the underlying immunological mechanisms through which the nitroslyating agent NORS provides protection against the development of BRDc in susceptible cattle. An in vitro BRDc experimental model was designed using bovine peripheral blood mononuclear cells (PBMCs) which were infected with bovine herpesvirus 1 (BHV-1) and subsequently cultured with lipopolysaccharides (LPS) extracted from Mannheimia haemolytica bacteria...
September 13, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27889877/maldi-tof-ms-meets-wgs-in-a-vre-outbreak-investigation
#15
S Schlebusch, G R Price, R L Gallagher, V Horton-Szar, L D H Elbourne, P Griffin, D J Venter, S O Jensen, S J Van Hal
The use of MALDI-TOF MS (matrix-assisted laser desorption/ ionization-time of flight mass spectrometry) and WGS (whole genome sequencing) has been described for identification and strain relatedness determination. We describe the complementary use of MALDI-TOF MS and WGS in a VRE (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. Although both assays indicated multiple clusters involved in the outbreak of vancomycin resistant Enterococcus faecium isolates from positive blood cultures of four haematology-oncology patients, the small cohort and discrepancies between findings indicate the limitations of MALDI-TOF MS and the cautious interpretation of MALDI-TOF MS dendrograms during outbreaks...
November 26, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27887865/overcoming-the-bottleneck-of-platelet-lysate-supply-in-large-scale-clinical-expansion-of-adipose-derived-stem-cells-a-comparison-of-fresh-versus-three-types-of-platelet-lysates-from-outdated-buffy-coat-derived-platelet-concentrates
#16
Peter V Glovinski, Mikkel Herly, Anders B Mathiasen, Jesper D Svalgaard, Rehannah Borup, Maj-Lis M Talman, Jens J Elberg, Stig-Frederik T Kølle, Krzysztof T Drzewiecki, Anne Fischer-Nielsen
BACKGROUND: Platelet lysates (PL) represent a promising replacement for xenogenic growth supplement for adipose-derived stem cell (ASC) expansions. However, fresh platelets from human blood donors are not clinically feasible for large-scale cell expansion based on their limited supply. Therefore, we tested PLs prepared via three methods from outdated buffy coat-derived platelet concentrates (PCs) to establish an efficient and feasible expansion of ASCs for clinical use. METHODS: PLs were prepared by the freeze-thaw method from freshly drawn platelets or from outdated buffy coat-derived PCs stored in the platelet additive solution, InterSol...
November 22, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27885249/lactation-related-microrna-expression-in-microvesicles-of-human-umbilical-cord-blood
#17
De-Jing Wang, Chen-Meiyi Wang, Yi-Ting Wang, Hai Qiao, Liao-Qiong Fang, Zhi-Biao Wang
BACKGROUND The complex process by which lactation is initiated upon neonate delivery remains incompletely understood. Microvesicles (MVs) can transmit microRNAs (miRNAs) into recipient cells to influence cell function, and recent studies have identified miRNAs essential for mammary gland development and lactation. This study aimed to investigate the expression of lactation-related miRNAs in MVs isolated from human umbilical cord blood immediately after delivery. MATERIAL AND METHODS Umbilical cord blood samples were collected from 70 healthy pregnant women, and MVs were isolated through differential centrifugation and characterized by transmission electron microscopy, Western blotting, and nanoparticle tracking analysis...
November 24, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27884338/the-effect-of-local-hyperglycemia-on-skin-cells-in%C3%A2-vitro-and-on-wound-healing-in-euglycemic-rats
#18
Carla R Kruse, Mansher Singh, Jens A Sørensen, Elof Eriksson, Kristo Nuutila
BACKGROUND: Multiple previous studies have established that high systemic blood glucose concentration impairs skin wound healing. However, the effects of local hyperglycemia on wound healing are not well defined. Comprehensive animal studies and in vitro studies using both fibroblasts and keratinocytes are lacking. MATERIALS AND METHODS: Primary keratinocytes and fibroblasts were isolated from discarded human tissue, cultured under different concentrations of glucose, and the effect on cell function was examined...
December 2016: Journal of Surgical Research
https://www.readbyqxmd.com/read/27884211/plasma-glutamine-levels-before-cardiac-surgery-are-related-to-post-surgery-infections-an-observational-study
#19
Hanneke Buter, Matty Koopmans, Ramses Kemperman, Lilian Jekel, Christiaan Boerma
BACKGROUND: A low plasma glutamine level was found in 34% of patients after elective cardiothoracic surgery. This could be a result of the inflammation caused by surgical stress or the use of extracorporeal circulation (ECC). But it is also possible that plasma glutamine levels were already lowered before surgery and reflect an impaired metabolic state and a higher likelihood to develop complications. In the present study plasma glutamine levels were measured before and after cardiac surgery and we questioned whether there is a relation between plasma glutamine levels and duration of ECC and the occurrence of postoperative infections...
November 25, 2016: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/27876571/fusobacterium-nucleatum-increases-proliferation-of-colorectal-cancer-cells-and-tumor-development-in-mice-by-activating-tlr4-signaling-to-nf%C3%AE%C2%BAb-upregulating-expression-of-microrna-21
#20
Yongzhi Yang, Wenhao Weng, Junjie Peng, Leiming Hong, Lei Yang, Yuji Toiyama, Renyuan Gao, Minfeng Liu, Mingming Yin, Cheng Pan, Hao Li, Bomin Guo, Qingchao Zhu, Qing Wei, Mary-Pat Moyer, Ping Wang, Sanjun Cai, Ajay Goel, Huanlong Qin, Yanlei Ma
BACKGROUND & AIMS: Nearly 20% of the global cancer burden can be linked to infectious agents. Fusobacterium nucleatum promotes tumor formation by epithelial cells via unclear mechanisms. We aimed to identify microRNAs (miRNAs) induced by F nucleatum and evaluate their ability to promote colorectal carcinogenesis in mice. METHODS: Colorectal cancer (CRC) cell lines were incubated with F nucleatum or control reagents and analyzed in proliferation and would healing assays...
November 19, 2016: Gastroenterology
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