keyword
MENU ▼
Read by QxMD icon Read
search

Blood culture timing

keyword
https://www.readbyqxmd.com/read/28529673/on-chip-two-photon-metabolic-imaging-for-drug-toxicity-testing
#1
Fang Yu, Shuangmu Zhuo, Yinghua Qu, Deepak Choudhury, Zhiping Wang, Ciprian Iliescu, Hanry Yu
We have developed a microfluidic system suitable to be incorporated with a metabolic imaging method to monitor the drug response of cells cultured on a chip. The cells were perfusion-cultured to mimic the blood flow in vivo. Label-free optical measurements and imaging of nicotinamide adenine dinucleotide and flavin adenine dinucleotide fluorescence intensity and morphological changes were evaluated non-invasively. Drug responses calculated using redox ratio imaging were compared with the drug toxicity testing results obtained with a traditional well-plate system...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28528569/time-to-treatment-and-mortality-during-mandated-emergency-care-for-sepsis
#2
Christopher W Seymour, Foster Gesten, Hallie C Prescott, Marcus E Friedrich, Theodore J Iwashyna, Gary S Phillips, Stanley Lemeshow, Tiffany Osborn, Kathleen M Terry, Mitchell M Levy
Background In 2013, New York began requiring hospitals to follow protocols for the early identification and treatment of sepsis. However, there is controversy about whether more rapid treatment of sepsis improves outcomes in patients. Methods We studied data from patients with sepsis and septic shock that were reported to the New York State Department of Health from April 1, 2014, to June 30, 2016. Patients had a sepsis protocol initiated within 6 hours after arrival in the emergency department and had all items in a 3-hour bundle of care for patients with sepsis (i...
May 21, 2017: New England Journal of Medicine
https://www.readbyqxmd.com/read/28526884/combined-use-of-metformin-and-atorvastatin-attenuates-atherosclerosis-in-rabbits-fed-a-high-cholesterol-diet
#3
Fei Luo, Yuan Guo, Gui-Yun Ruan, Jun-Ke Long, Xi-Long Zheng, Qin Xia, Shui-Ping Zhao, Dao-Quan Peng, Zhen-Fei Fang, Xiang-Ping Li
Statins are widely used to reduce cardiovascular risk. Unfortunately, some patients still experience cardiovascular events though prescribed with high-intensity statins. Metformin, an anti-diabetic drug, was reported to possess anti-atherosclerotic effects. Therefore, the experiments were designed to evaluate whether combined use of metformin and atorvastatin can achieve additional benefits. In rabbits fed a high-cholesterol diet, we evaluated the effects of the combination therapy on atherosclerotic plaques, lipid profiles, blood glucose levels, liver and kidney functions...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526564/direct-detection-of-anaplasma-phagocytophilum-by-polymerase-chain-reaction-followed-by-electrospray-ionization-mass-spectrometry-from-human-blood
#4
Heimo Lagler, Nicole Harrison, Manuel Kussmann, Markus Obermüller, Heinz Burgmann, Athanasios Makristathis, Michael Ramharter
Bacterial pathogens not detectable in commercial blood culture assays constitute an important challenge for infectious disease physicians, in particular if clinical symptoms of the illness are unspecific. In this report Anaplasma phagocytophilum was detected directly from a peripheral blood sample of a febrile patient reporting a tick bite by a commercial system based on polymerase chain reaction followed by electrospray ionization mass spectrometry. Therefore, the diagnosis of human granulocytic anaplasmosis infection was established by this diagnostic methodology for the first time...
May 16, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28526067/the-chimeric-monoclonal-antibody-mhcsz-123-against-human-von-willebrand-factor-a3-domain-inhibits-high-shear-arterial-thrombosis-in-a-rhesus-monkey-model
#5
Shundong Ji, Miao Jiang, Bin Yan, Fei Shen, Yang He, Aini Wan, Lijun Xia, Changgeng Ruan, Yiming Zhao
BACKGROUND: SZ-123, a murine monoclonal antibody that targets the human von Willebrand factor (VWF) A3 domain and blocks the binding of collagen, is a powerful antithrombotic. In a Rhesus monkey model of thrombosis, SZ-123 had no side effects, such as bleeding or thrombocytopenia. METHODS: The mouse/human chimeric version of SZ-123, MHCSZ-123, was developed and maintained inhibitory capacities in vitro and ex vivo after injection into monkeys. CHO-S cells were selected for stable expression of MHCSZ-123...
May 19, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28514968/further-evaluation-of-the-nwf-filter-for-the-purification-of-plasmodium-vivax-infected-erythrocytes
#6
Jiangyan Li, Zhiyong Tao, Qian Li, Awtum Brashear, Ying Wang, Hui Xia, Qiang Fang, Liwang Cui
BACKGROUND: Isolation of Plasmodium-infected red blood cells (iRBCs) from clinical blood samples is often required for experiments, such as ex vivo drug assays, in vitro invasion assays and genome sequencing. Current methods for removing white blood cells (WBCs) from malaria-infected blood are time-consuming or costly. A prototype non-woven fabric (NWF) filter was developed for the purification of iRBCs, which showed great efficiency for removing WBCs in a pilot study. Previous work was performed with prototype filters optimized for processing 5-10 mL of blood...
May 17, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28513775/adiponectin-inhibits-oxidization-induced-differentiation-of-t-helper-cells-through-inhibiting-costimulatory-cd40-and-cd80
#7
Y Xiao, T Deng, Z Shang, D Wang
Adiponectin is a multifunctional adipokine that has several oligomeric forms in the blood stream, which broadly regulates innate and acquired immunity. Therefore, in this study, we aimed to observe the differentiation of T helper (Th) cells and expression of costimulatory signaling molecules affected by adiponectin. The mRNA and protein expression levels of adiponectin and its receptors in oxidized low density lipoprotein cholesterol-treated endothelial cells were assayed by real time PCR and immunofluorescence...
May 15, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28512861/rapid-diagnosis-of-sepsis-with-taqman-based-multiplex-real-time-pcr
#8
Chang-Feng Liu, Xin-Ping Shi, Yun Chen, Ye Jin, Bing Zhang
BACKGROUND: The survival rate of septic patients mainly depends on a rapid and reliable diagnosis. A rapid, broad range, specific and sensitive quantitative diagnostic test is the urgent need. Thus, we developed a TaqMan-Based Multiplex real-time PCR assays to identify bloodstream pathogens within a few hours. METHODS: Primers and TaqMan probes were designed to be complementary to conserved regions in the 16S rDNA gene of different kinds of bacteria. To evaluate accurately, sensitively, and specifically, the known bacteria samples (Standard strains, whole blood samples) are determined by TaqMan-Based Multiplex real-time PCR...
May 17, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28509314/influence-of-oxygen-concentration-on-t-cell-proliferation-and-susceptibility-to-apoptosis-in-healthy-men-and-women
#9
Agnieszka Waskowska, Katarzyna A Lisowska, Agnieszka Daca, Izabella Henc, Fredrik Brandberg, Paula Mazurek, Edyta Brzustewicz, Jacek M Witkowski, Ewa Bryl
INTRODUCTION: Much of what we know about the functioning of human T lymphocytes is based on the experiments carried out in atmospheric oxygen (O₂) concentrations, which are significantly higher than those maintained in blood. Interestingly, the gender differences in the activity of T cells and their susceptibility to apoptosis under different O₂ conditions have not yet been described. The aim of the study was to compare two main markers of lymphocyte function: proliferation capacity and ability to produce cytokines as well as their susceptibility to apoptosis under two different O₂ concentrations, between men and women...
2017: Folia Histochemica et Cytobiologica
https://www.readbyqxmd.com/read/28507940/cord-blood-cells-responses-to-il2-il7-and-il15-cytokines-for-mtor-expression
#10
Anahita Mohammadian, Elahe Naderali, Seyedeh Momeneh Mohammadi, Aliakbar Movasaghpour, Behnaz Valipour, Mohammad Nouri, Hojjatollah Nozad Charoudeh
Purpose: Mammalian target of rapamycin (mTOR)is important in hematopoiesis and affect cell growth,differentiation and survival. Although previous studies were identified the effect of cytokines on the mononuclear cells development however the cytokines effect on mTOR in cord blood mononuclear cells was unclear. The aim of this study was to evaluate mTOR expression in cord blood mononuclear and cord blood stem cells (CD34(+) cells) in culture conditions for lymphoid cell development. Methods: Isolation of The mononuclear cells (MNCs) from umbilical cord blood were done with use of Ficollpaque density gradient...
April 2017: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28506845/ex-vivo-mesenchymal-precursor-cell-expanded-cord-blood-transplantation-following-reduced-intensity-conditioning-regimens-improves-time-to-neutrophil-recovery
#11
Rohtesh S Mehta, Rima M Saliba, Kai Cao, Indreshpal Kaur, Katy Rezvani, Julianne Chen, Amanda Olson, Simrit Parmar, Nina Shah, David Marin, Amin Alousi, Chitra Hosing, Uday Popat, Partow Kebriaei, Richard Champlin, Marcos de Lima, Donna Skerrett, Elizabeth Burke, Elizabeth J Shpall, Betul Oran
We previously showed the safety of using cord blood (CB) expanded ex vivo in cocultures with allogeneic mesenchymal precursor cells (MPC) after myeloablative conditioning with faster recovery of neutrophils and platelets compared with historical controls. Herein, we report the transplant outcomes of 27 patients with hematologic cancers who received one CB unit ex vivo expanded with MPCs in addition to an unmanipulated CB (MPC group) after reduced intensity conditioning (RIC). The results in this group were compared with 51 historical controls who received two unmanipulated CB units (control group)...
May 12, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/28505046/south-asians-why-are-they-at-a-higher-risk-for-cardiovascular-disease
#12
Anjly Jain, Rajeev Puri, Devaki R Nair
PURPOSE OF REVIEW: We comment on the high prevalence of cardiovascular disease (CVD) in South Asians (SA). The effect of various risk factors, for example biochemical, genetic, lifestyle, socioeconomic factors and psychosocial stress on CVD risk is discussed. RECENT FINDINGS: 'Prediabetes' is common in SA, but its relationship with coronary artery disease (CAD) is not significant unlike for the white population. At the same time, 'prediabetes' in SA is associated with an increased risk for cerebrovascular disease (CeVD)...
May 12, 2017: Current Opinion in Cardiology
https://www.readbyqxmd.com/read/28504280/metabolic-profile-and-underlying-improved-bio-activity-of-fructus-aurantii-immaturus-by-human-intestinal-bacteria
#13
Xing Yan Liu, Meng Lin Fan, Huai You Wang, Bo Yang Yu, Ji Hua Liu
Fructus aurantii immaturus (FAI) is the dried young fruit of Citrus aurantium L. or Citrus sinensis L. Osbeck. The purpose of this paper was to investigate the metabolic fate of FAI upon incubation with human intestinal bacteria, meanwhile to evaluate the antioxidant and anti-inflammatory activities of FAI and the transformed Fructus aurantii immaturus (TFAI). The water extract of FAI was anaerobically incubated with human intestinal bacterial suspensions for 48 h at 37 °C. Liquid chromatography-hybridised with quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) was applied to identify FAI metabolites...
May 15, 2017: Food & Function
https://www.readbyqxmd.com/read/28502395/multicenter-evaluation-of-the-rapidec%C3%A2-carba-np-test-for-rapid-screening-of-carbapenemase-producing-enterobacteriaceae-and-gram-negative-nonfermenters-from-clinical-specimens
#14
Marco Coppi, Alberto Antonelli, Tommaso Giani, Teresa Spanu, Flora Marzia Liotti, Carla Fontana, Walter Mirandola, Raffaele Gargiulo, Agostino Barozzi, Carola Mauri, Luigi Principe, Gian Maria Rossolini
The rapid diagnosis of carbapenemase-producing (CP) bacteria is essential for the management of therapy and infection control. In this study, RAPIDEC® CARBA NP (RCNP) was evaluated for the rapid screening of CP Enterobacteriaceae, Acinetobacter baumannii complex, and Pseudomonas aeruginosa from clinical specimens collected at five Italian hospitals. Firstly, each site tested 20 well-characterized strains in a blinded fashion. Secondly, each center prospectively tested 25 isolates from blood cultures processed with a rapid workflow (6h after subculture) and 25 isolates from other specimens processed after an overnight culture...
April 27, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28501726/osteogenic-differentiation-of-mesenchymal-stem-cells-is-impaired-by-bone-morphogenetic-protein-7
#15
Paweena Wongwitwichot, Jasadee Kaewsrichan
PURPOSE: Mesenchymal stem cells (MSCs) are multipotent adult stem cells and present in practically all tissues but originally identified within the bone marrow (BM). The differentiation potential of these cells is generally impaired when culturing in vitro for cell expansion. The aim of this study is to speedily increase the numbers of bone marrow derived mesenchymal stem cells (BM-MSCs) with substantially maintaining their differentiation potential in vitro and improving bone formation in vivo...
May 11, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28500506/a-controlled-comparison-of-the-bact-alert%C3%A2-3d-and-virtuo%C3%A2-microbial-detection-systems
#16
H Totty, M Ullery, J Spontak, J Viray, M Adamik, B Katzin, W M Dunne, P Deol
The performance of the next-generation BacT/ALERT® VIRTUO™ Microbial Detection System (VIRTUO™, bioMérieux Inc., Hazelwood, MO) was compared to the BacT/ALERT® 3D Microbial Detection System (3D, bioMérieux Inc., Durham, NC) using BacT/ALERT® FA Plus (FA Plus), BacT/ALERT® PF Plus (PF Plus), BacT/ALERT® FN Plus (FN Plus), BacT/ALERT® Standard Aerobic (SA), and BacT/ALERT® Standard Anaerobic (SN) blood culture bottles (bioMérieux Inc., Durham, NC). A seeded limit of detection (LoD) study was performed for each bottle type in both systems...
May 12, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28497617/association-of-npr3-polymorphism-with-risk-of-essential-hypertension-in-a-chinese-population
#17
D-B Kuang, J-P Zhou, M-P Li, J Tang, X-P Chen
WHAT IS KNOWN AND OBJECTIVE: Essential hypertension (EH) is a common disease exhibiting large individual difference in occurrence, development and treatment response. Genetic factors are implicated in the development and progression of EH. This study aimed to explore the association between NPR3 single nucleotide polymorphism rs2270915 (A/G, Asn521Asp) and the risk of EH in a Chinese Han population by a case-control study. METHODS: The study was a single-centre, case-control trial, in which a total of 287 EH patients and 289 age- and sex-matched healthy controls were enrolled...
May 11, 2017: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/28495564/characterization-of-toll-like-receptor-22-in-turbot-scophthalmus-maximus
#18
Jing Xing, Xiujuan Zhou, Xiaoqian Tang, Xiuzhen Sheng, Wenbin Zhan
Toll-like receptors (TLRs) are essential for activation of the innate immune system in response to invading pathogens. In this paper, expression profiles of the turbot (Scophthalmus maximus) TLR22 gene (tbTLR22) were analyzed with RT-PCR and in situ hybridization. Then its expression patterns simulated with ligands or pathogens were investigated. Streptococcus iniae, Edwardsiella tarda, Hirame rhabdovirus virus (HIRRV), polyinosinic: polycytidylic acid (Poly I:C), peptidoglycan (PGN), or lipopolysaccharides (LPS) was injected to turbot; poly I:C, PGN, or LPS was added into cultured peripheral blood leukocytes (PBL); and then the tbTLR22 in liver, spleen, gill, kidney and cultured PBL was measured using Quantitative PCR...
May 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28492525/neonatal-sepsis-in-rural-india-timing-microbiology-and-antibiotic-resistance-in-a-population-based-prospective-study-in-the-community-setting
#19
P Panigrahi, D S Chandel, N I Hansen, N Sharma, S Kandefer, S Parida, R Satpathy, L Pradhan, A Mohapatra, S S Mohapatra, P R Misra, N Banaji, J A Johnson, J G Morris, I H Gewolb, R Chaudhry
OBJECTIVE: To examine the timing and microbiology of neonatal sepsis in a population-based surveillance in the Indian community setting. STUDY DESIGN: All live born infants in 223 villages of Odisha state were followed at home for 60 days. Suspect sepsis cases were referred to study hospitals for further evaluation including blood culture. RESULTS: Of 12 622 births, 842 were admitted with suspected sepsis of whom 95% were 4 to 60 days old...
May 11, 2017: Journal of Perinatology: Official Journal of the California Perinatal Association
https://www.readbyqxmd.com/read/28491325/propionimicrobium-lymphophilum-and-actinotignum-schaalii-bacteraemia-a-case-report
#20
M Ikeda, T Kobayashi, T Suzuki, Y Wakabayashi, Y Ohama, S Maekawa, S Takahashi, Y Homma, K Tatsuno, T Sato, S Okugawa, K Moriya, H Yotsuyanagi
Propionimicrobium lymphophilum is an anaerobic Gram-positive bacillus that exists in human skin and urinary tract. The pathogenicity is, however, not well known. Only two cases of urinary tract infection have been described recently. In the case presented here, the bacterium was isolated, concomitant with Actinotignum schaalii, from blood culture of a patient with fever and difficulty of urination. The bacteria were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA sequencing...
July 2017: New Microbes and New Infections
keyword
keyword
15735
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"