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Blood culture timing

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https://www.readbyqxmd.com/read/29168187/storage-time-of-platelet-concentrates-and-risk-of-a-positive-blood-culture-a-nationwide-cohort-study
#1
Aukje L Kreuger, Klaus Rostgaard, Rutger A Middelburg, Jean-Louis H Kerkhoffs, Gustav Edgren, Christian Erikstrup, Ole B Pedersen, Kjell Titlestad, Kaspar R Nielsen, Sisse R Ostrowski, Marianne Voldstedlund, Johanna G van der Bom, Henrik Ullum, Henrik Hjalgrim
BACKGROUND: Concern of transfusion-transmitted bacterial infections has been the major hurdle to extend shelf life of platelet (PLT) concentrates. We aimed to investigate the association between storage time and risk of positive blood cultures at different times after transfusion. STUDY DESIGN AND METHODS: We performed a nationwide cohort study among PLT transfusion recipients in Denmark between 2010 and 2012, as recorded in the Scandinavian Donations and Transfusions (SCANDAT2) database...
November 22, 2017: Transfusion
https://www.readbyqxmd.com/read/29167141/impact-of-automatic-infectious-diseases-consultation-on-the-management-of-fungemia-at-a-large-academic-medical-center
#2
Travis M Jones, Richard H Drew, Dustin T Wilson, Christina Sarubbi, Deverick J Anderson
PURPOSE: The impact of automatic infectious diseases (ID) consultation for inpatients with fungemia at a large academic medical center was studied. METHODS: In this single-center, retrospective study, the time to appropriate antifungal therapy before and after implementing a policy requiring automatic ID consultation for the management of fungemia for all patients with an inpatient positive blood culture for fungus was examined. The rates of ID consultation; the likelihood of receiving appropriate antifungal therapy; central venous catheter (CVC) removal rates; performance of ophthalmologic examinations; infection-related length of stay (LOS); rates of all-cause inhospital mortality, death, or transfer to an intensive care unit within 7 days of first culture; and inpatient cost of antifungals were also evaluated...
December 1, 2017: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/29165229/na%C3%A3-ve-t-cells-are-activated-by-autologous-hcmv-infected-endothelial-cells-through-nkg2d-and-can-control-hcmv-transmission-in-vitro
#3
Narmadha Subramanian, Zeguang Wu, Frank Reister, Kerstin Laib Sampaio, Giada Frascaroli, Luka Cicin-Sain, Thomas Mertens
Apart from classical antigen-presenting cells (APCs) like dendritic cells and macrophages, there are semiprofessional APCs such as endothelial cells (ECs) and Langerhans' cells. Human cytomegalovirus (HCMV) infects a wide range of cell types including the ECs which are involved in the trafficking and homing of T cells. By investigating the interaction of naïve T cells obtained from HCMV-seronegative umbilical cord blood with autologous HCMV-infected human umbilical vein ECs (HUVECs), we could show that the activation of naïve T cells occurred after 1 day of peripheral blood mononuclear cell (PBMC) exposure to HCMV-infected HUVECs...
November 21, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29164082/acute-septic-arthritis-of-the-knee-caused-by-kingella-kingae-in-a-5-year-old-cameroonian-boy
#4
Nawal El Houmami, Dimitri Ceroni, Karine Codjo Seignon, Jean-Christophe Pons, Cédric Lambert, Guillaume André Durand, Philippe Minodier, Léopold Lamah, Philippe Bidet, Jacques Schrenzel, Didier Raoult, Pierre-Edouard Fournier
Kingella kingae is an important cause of invasive infections in young children from Western countries. Although increasing reports indicate that this organism is the leading agent of bone and joint infections in early childhood, data on K. kingae infections from resource-limited settings are scarce, and none has yet been reported in Africa. We herein report the diagnostic and epidemiological investigations of the first case of K. kingae arthritis identified in a child from sub-Saharan Africa. A 5-year-old Cameroonian boy presented with a sudden painful limp which appeared in the course of a mild rhinopharyngitis...
2017: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/29161941/are-we-missing-the-staphylococcus-aureus-bacteraemia-forest-for-the-mrsa-trees
#5
Kevin B Laupland
In this edition of the journal, Jokinen et al. report on a population based study of Staphylococcus aureus bacteraemia (SAB) in Finland. They observe increasing incidence of cases over time that are associated with an increase in blood culture sampling. As compared to methicillin-sensitive SAB that increases during the study period, the incidence of methicillin-resistant SAB increases and then decreases. The proportion of cases with penicillin-sensitive SAB markedly increases in the study representing 43% of isolates in the most recent year of surveillance...
November 21, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/29158893/evaluation-of-a-stenotrophomonas-maltophilia-bacteremia-cluster-in-hematopoietic-stem-cell-transplantation-recipients-using-whole-genome-sequencing
#6
Stefanie Kampmeier, Mike H Pillukat, Aleksandra Pettke, Annelene Kossow, Evgeny A Idelevich, Alexander Mellmann
Background: Stenotrophomonas maltophilia ubiquitously occurs in the hospital environment. This opportunistic pathogen can cause severe infections in immunocompromised hosts such as hematopoietic stem cell transplantation (HSCT) recipients. Between February and July 2016, a cluster of four patients on the HSCT unit suffered from S. maltophilia bloodstream infections (BSI). Methods: For epidemiological investigation we retrospectively identified the colonization status of patients admitted to the ward during this time period and performed environmental monitoring of shower heads, shower outlets, washbasins and toilets in patient rooms...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29158327/early-onset-group-b-streptococcus-eogbs-infection-subsequent-to-cessation-of-screening-based-intrapartum-prophylaxis-findings-of-an-observational-study-in-west-london-uk
#7
Guduru Gopal Rao, Jane Townsend, Daniel Stevenson, Grace Nartey, Stephen Hiles, Paul Bassett, Theresa Lamagni, Richard Nicholl
OBJECTIVES: To describe the impact on early-onset group B Streptococcus (EOGBS) infection rates following reversion from screening-based to risk-based intrapartum antimicrobial prophylaxis (IAP) for prevention. SETTING: Maternity services provided by secondary healthcare organisation in North West London. PARTICIPANTS: All women who gave birth in the healthcare organisation between April 2016 and March 2017. There were no exclusions. DESIGN: Observational study comparing EOGBS rates in the postscreening period (2016-2017) with prescreening (2009-2013) and screening periods (2014-2015)...
November 19, 2017: BMJ Open
https://www.readbyqxmd.com/read/29157318/effect-of-clinical-variables-on-the-volume-of-blood-collected-for-blood-cultures-in-an-adult-patient-population
#8
R Logan Jones, Harlan R Sayles, Paul D Fey, Mark E Rupp
OBJECTIVE To identify clinical variables that influence blood culture volume recovery DESIGN Retrospective chart review and linear model analysis SETTING A 621-bed Academic Medical Center with a Clinical Laboratory that processes 20,000+ blood cultures annually and dedicated phlebotomy staff for venipuncture PATIENTS Consecutive patients requiring blood culture METHODS Over a 6-day period, blood volume was determined in 568 culture bottles from 128 unique adult patients, and clinical data from the time of phlebotomy were extracted from hospital electronic medical records...
November 21, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29157270/autologous-platelet-rich-plasma-induces-bone-formation-of-tissue-engineered-bone-with-bone-marrow-mesenchymal-stem-cells-on-beta-tricalcium-phosphate-ceramics
#9
Tengbo Yu, Huazheng Pan, Yanling Hu, Hao Tao, Kai Wang, Chengdong Zhang
BACKGROUND: The purpose of the study is to investigate whether autologous platelet-rich plasma (PRP) can serve as bone-inducing factors to provide osteoinduction and improve bone regeneration for tissue-engineered bones fabricated with bone marrow mesenchymal stem cells (MSCs) and beta-tricalcium phosphate (β-TCP) ceramics. The current study will give more insight into the contradictory osteogenic capacity of PRP. METHODS: The concentration of platelets, platelet-derived growth factor-AB (PDGF-AB), and transforming growth factor-β1 (TGF-β1) were measured in PRP and whole blood...
November 21, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29155394/label-free-and-dynamic-monitoring-of-cytotoxicity-to-the-blood-brain-barrier-cells-treated-with-nanometre-copper-oxide
#10
Duan Lian, Zhang Chonghua, Gu Wen, Zhang Hongwei, Bai Xuetao
A cytotoxicity study was conducted with a primary culture of the nervous system cells, including brain microvascular endothelial cells (BMECs) and astrocytes, which are important components of the blood-brain barrier. The real-time cell analysis (RTCA) was used to determine the cytotoxicity of copper-oxide nanoparticles (CuO NPs). The IC50 values of CuO NPs in astrocytes and BMECs were determined by the RTCA at different exposure times and were used as base values for further research. DNA damage after exposure to CuO NPs for 3 and 24 h was assessed using comet assay at the IC50 obtained from RTCA...
December 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29154506/evaluation-of-major-and-minor-lower-extremity-amputation-in-diabetic-foot-patients
#11
Fırat Ozan, Kaan Gürbüz, İlhami Çelik, Zehra Beştepe Dursun, Erdal Uzun
Background/aim: We evaluated the existing risk factors with clinical results in patients who underwent major and minor amputation of the lower extremity as a result of diabetic foot ulcers (DFUs). Materials and methods: We retrospectively studied 107 patients who had undergone lower extremity amputation. The patients were divided into minor (Group 1, n = 75) and major (Group 2, n = 32) amputation groups. On clinical evaluation, the type of surgery performed, smoking history, comorbidities, duration of diabetes mellitus (DM) diagnosis, duration of DFU presence, peripheral neuropathy, peripheral arterial disease, results of deep tissue culture, length of hospitalization, and blood parameters were investigated...
August 23, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29153954/mir-150-5p-regulates-egr2-to-promote-the-development-of-chronic-rhinosinusitis-via-the-dc-th-axis
#12
Zuxia Ma, Yang Shen, Quan Zeng, Jie Liu, Li Yang, Ran Fu, Guohua Hu
BACKGROUND AND AIMS: Accumulating studies indicate that miR-150-5p might play a significant role in dendritic cells (DCs) of peripheral blood in chronic rhinosinusitis (CRS) patients. We sought to investigate the effects and mechanism of miR-150-5p, which regulates early growth response 2 (EGR2) to promote the development of CRS via the DC-Th axis. METHODS: The upregulated expression of miR-150-5p in DCs of CRS was assayed by real-time quantitative polymerase chain reaction (qRT-PCR), and IL-17 cytokines in the supernatants of DC-naïve T cells co-cultures were analysed by enzyme-linked immunosorbent assay (ELISA)...
November 15, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29153062/-diagnostic-accuracy-of-the-lightcycler%C3%A2-septifast-assay-in-the-childhood-febrile-neutropenia
#13
Esra Mimaroğlu, Elvan Çağlar Çıtak, Necdet Kuyucu, Gönül Arslan
Infection is the main problem among the patients receiving cancer therapy. The mortality rate can be reduced by the appropriate treatment in the right time. Although blood culture is the gold standard for the diagnoses of sepsis, many factors influence the results of blood culture in children. For this reason, real time polymerase chain reaction (Rt-PCR) has gained importance for the diagnoses of microbiological agents as it is faster than the conventional methods. In this study, we aimed to compare the efficacy of SeptiFast (SF) test with blood culture among children with neutropenic fever...
October 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29151418/-vegf165-transfected-endothelial-progenitor-cells-mediated-by-lentivirus-alleviated-ali-in-rats
#14
Zhaohui He, Huiwei He, Yuanhua Lu, Zhi Chen, Fanghua Xu, Rongsheng Wang, Chunli Yang
OBJECTIVE: To investigate the protective effects of vascular endothelial growth factor-165 (VEGF165) transfected the endothelial progenitor cells (EPCs) mediated by lentivirus on acute lung injury (ALI) in rats. METHODS: The mononuclear cells from the male Sprague-Dawley (SD) rats were isolated and cultured to get the EPCs for study. The lentivirus vector carrying the human VEGF165 gene was constructed. According to the random number table method, 90 male SD rats were divided into ALI model group, phosphate buffer solution (PBS) group, EPCs treatment group, none transfected EPCs treatment group and VEGF165 transfected EPCs treatment group, and the rats in each group were subdivided into 4, 12 and 48 hours subgroups, with 6 rats in each subgroup...
November 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29151286/-experimental-study-of-glioma-stem-cell-mediated-immune-tolerance-in-tumor-microenvironment
#15
T Xie, J W Ma, B Liu, J Dong, Q Huang
Objective: To investigate the tumor microenvironment of immune tolerance induced by glioma stem cells (GSC). Methods: Human GSC SU3 cells transfected with red fluorescent protein (SU3-RFP) gene were implanted into the brain, subcutis (armpit and foot), liver and abdominal cavity of transgenic green fluorescence protein (GFP) nude mice to establish RFP(+) /GFP(+) dual fluorescence solid tumor model. The re-cultured cells derived from implanted tumor tissues, SU3-RFP cells co-cultured with peritoneal fluid of transgenic GFP nude mice and malignant ascites of tumor-bearing mice were observed by fluorescence microscopy and real-time video image tracing to analyze the microenvironment of immune tolerance mediated by RFP(+) /GFP(+) implanted tumor...
November 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/29150642/rescue-of-pfos-induced-human-sertoli-cell-injury-by-overexpressing-a-p-fak-y407e-phosphomimetic-mutant
#16
Haiqi Chen, Ying Gao, Dolores D Mruk, Xiang Xiao, Constance M John, Paul J Turek, Wing-Yee Lui, Will M Lee, Bruno Silvestrini, C Yan Cheng
PFOS induces Sertoli cell injury using testicular cells isolated from rodent testes, but it remains unknown if PFOS has similar effects in humans. Herein, we maintained human Sertoli cells in a mitotically active state in vitro, thus enabling transfection experiments that altered gene expression to explore the molecular mechanism(s) underlying toxicant-induced cell injury. Human Sertoli cells obtained from men at ages 15, 23, 36 and 40 were cultured in vitro. These differentiated Sertoli cells remained mitotically active when cultured in the presence of 10% FBS (fetal bovine serum), with a replication time of ~1-3 weeks...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146490/educational-inequalities-in-cardiovascular-risk-factor-and-blood-pressure-control-in-elderly-adults-comparison-of-mesa-cohort-and-chilean-nhs-survey-outcome-measures
#17
Carolina Nazzal, Steven Shea, Cecilia Castro-Diehl, Tania Alfaro, Patricia Frenz, Carlos J Rodriguez
BACKGROUND: Social determinants differ between countries, which is not always considered when adapting health policies and interventions to face inequalities in noncommunicable diseases and their risk factors. OBJECTIVES: The study sought to analyze educational inequalities in controlled blood pressure (CBP), obesity, and smoking in study populations from Chile and the United States in 2 periods, both countries with large social inequalities. METHODS: The study used data from the first and fifth waves of the MESA (Multiethnic Study of Atherosclerosis) cohort, and the 2003 and 2009 to 2010 Chilean National Health Survey (CNHS) survey outcome measures...
November 13, 2017: Global Heart
https://www.readbyqxmd.com/read/29143796/angelica-sinensis-polysaccharides-ameliorate-stress-induced-premature-senescence-of-hematopoietic-cell-via-protecting-bone-marrow-stromal-cells-from-oxidative-injuries-caused-by-5-fluorouracil
#18
Hanxianzhi Xiao, Lirong Xiong, Xiaoying Song, Pengwei Jin, Linbo Chen, Xiongbin Chen, Hui Yao, Yaping Wang, Lu Wang
Myelosuppression is the most common complication of chemotherapy. Decline of self-renewal capacity and stress-induced premature senescence (SIPS) of hematopoietic stem cells (HSCs) induced by chemotherapeutic agents may be the cause of long-term myelosuppression after chemotherapy. Whether the mechanism of SIPS of hematopoietic cells relates to chemotherapeutic injury occurred in hematopoietic microenvironment (HM) is still not well elucidated. This study explored the protective effect of Angelica sinensis polysaccharide (ASP), an acetone extract polysaccharide found as the major effective ingredients of a traditional Chinese medicinal herb named Chinese Angelica (Dong Quai), on oxidative damage of homo sapiens bone marrow/stroma cell line (HS-5) caused by 5-fluorouracil (5-FU), and the effect of ASP relieving oxidative stress in HM on SIPS of hematopoietic cells...
October 28, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29142077/syndromic-panel-based-testing-in-clinical-microbiology
#19
REVIEW
Poornima Ramanan, Alexandra L Bryson, Matthew J Binnicker, Bobbi S Pritt, Robin Patel
The recent development of commercial panel-based molecular diagnostics for the rapid detection of pathogens in positive blood culture bottles, respiratory specimens, stool, and cerebrospinal fluid has resulted in a paradigm shift in clinical microbiology and clinical practice. This review focuses on U.S. Food and Drug Administration (FDA)-approved/cleared multiplex molecular panels with more than five targets designed to assist in the diagnosis of bloodstream, respiratory tract, gastrointestinal, or central nervous system infections...
January 2018: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/29141775/impact-of-interventions-by-an-antimicrobial-stewardship-program-team-on-appropriate-antimicrobial-therapy-in-patients-with-bacteremic-urinary-tract-infection
#20
Mitsuru Yanai, Maiko Ogasawara, Yuta Hayashi, Kiyozumi Suzuki, Hiromichi Takahashi, Atsushi Satomura
BACKGROUND: Inappropriate antimicrobial therapy often leads to poor outcomes. This study aimed to evaluate the impact of an antimicrobial stewardship program (ASP) team on appropriate therapy, in patients with bacteremic urinary tract infection (UTI). PATIENTS AND METHODS: We retrospectively reviewed the interventions by the ASP team in 807 patients with bacteremic UTI. Interventions were divided into 3 groups: group A (conventional report), group B (conventional report and written alert on the chart), and group C (conventional report and oral recommendation with/without written alert)...
November 12, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
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