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https://www.readbyqxmd.com/read/28330315/characterization-and-development-of-est-ssr-markers-in-sweet-potato-ipomoea-batatas-l-lam
#1
Jin-Hee Kim, Jun-Hoi Kim, Won-Sam Jo, Jeong-Gwan Ham, Il Kyung Chung, Kyung-Min Kim
In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The lengths of the developed EST-SSR markers ranged from 100 to 499 bp (average length 238 bp). Their motif sequence types were varied, with most being dinucleotides and pentanucleotides, and the most commonly found motifs were CAGAAT (29...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330243/micropropagation-and-validation-of-genetic-and-biochemical-fidelity-among-regenerants-of-nothapodytes-nimmoniana-graham-mabb-employing-issr-markers-and-hplc
#2
Lokesh Prakash, Sushil Kumar Middha, Sudipta Kumar Mohanty, Mallappa Kumara Swamy
An in vitro protocol has been established for clonal propagation of Nothapodytes nimmoniana which is an important source of Camptothecin (CPT). Elite source was identified based on the chemical potency to accumulate the optimum level of CPT. Different types and concentrations of plant growth regulators were used to study their effect on inducing multiple shoots from the explants regenerated from embryos of N. nimmoniana. Of these, a combination of N6-benzyladenine (0.2 mg L(-1)) and Indole-3-butyric acid (IBA) (0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330228/development-of-novel-ssr-markers-for-evaluation-of-genetic-diversity-and-population-structure-in-tribulus-terrestris-l-zygophyllaceae
#3
Kuljit Kaur, Vikas Sharma, Vijay Singh, Mohammad Saleem Wani, Raghbir Chand Gupta
Tribulus terrestris L., commonly called puncture vine and gokhru, is an important member of Zygophyllaceae. The species is highly important in context to therapeutic uses and provides important active principles responsible for treatment of various diseases and also used as tonic. It is widely distributed in tropical regions of India and the world. However, status of its genetic diversity remained concealed due to lack of research work in this species. In present study, genetic diversity and structure of different populations of T...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330142/an-overview-of-physico-chemical-mechanisms-of-biogas-production-by-microbial-communities-a-step-towards-sustainable-waste-management
#4
REVIEW
Ramansu Goswami, Pritam Chattopadhyay, Arunima Shome, Sambhu Nath Banerjee, Amit Kumar Chakraborty, Anil K Mathew, Shibani Chaudhury
Biogas is a combination of methane, CO2, nitrogen, H2S and traces of few other gases. Almost any organic waste can be biologically transformed into biogas and other energy-rich organic compounds through the process of anaerobic digestion (AD) and thus helping in sustainable waste management. Although microbes are involved in each step of AD, knowledge about those microbial consortia is limited due to the lack of phylogenetic and metabolic data of predominantly unculturable microorganisms. However, culture-independent methods like PCR-based ribotyping has been successfully employed to get information about the microbial consortia involved in AD...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330095/potential-mosquito-repellent-compounds-of-ocimum-species-against-3n7h-and-3q8i-of-anopheles-gambiae
#5
Venugopal Gaddaguti, Talluri Venkateswara Rao, Allu Prasada Rao
Mosquitoes are exceptionally efficient in detecting their hosts for blood meal using odorant binding proteins, viz. 3N7H and 3Q8I and spread several dreadful diseases. DEET is a synthetic mosquito repellent widely used all over world for protection against mosquito bite. Reports reveal that, synthetic mosquito repellents may pose health problems in considerably large population. In view of the above fact, we made an attempt to discover efficient and novel natural mosquito repellent compounds with least impact on human health...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329944/-evolutionary-characteristics-and-positive-selection-site-of-hepatitis-c-virus-isolated-in-intravenous-drug-users-in-pudong-new-district-shanghai
#6
Y Wang, H Y Wu, X C Zhao, W P Zhu, Q Wan, Y H Lu, Q W Jiang
Objective: To understand the genotypes of hepatitis C virus (HCV) circulating in intravenous drug users (IDUs) in Pudong new district, Shanghai, and explore the population growth and selection pressure of the HCV strains isolated. Methods: A total of 200 serum specimens sampled from IDUs in local methadone maintenance treatment clinic in Pudong were used for amplification of a HCV NS5B 377-nt partial sequence. Mean evolutionary rate and effective number of infections were estimated based on the 377-nt partial sequences of the HCV strains isolated from IDUs and isolated contemporarily from local voluntary blood donors, men who have sex with men and reported hepatitis C cases by using BEAST software...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329861/varying-appraisals-of-elder-mistreatment-among-victims-findings-from-a-population-based-study
#7
David Burnes, Mark S Lachs, Denise Burnette, Karl Pillemer
Objectives: Prior elder mistreatment (EM) research has not examined subjective assessments of problem seriousness from the perspective of victims. This study sought to describe the variation in appraisals of perceived EM seriousness among victims of emotional abuse, physical abuse, and neglect and to examine factors that influence varying appraisals using neutralization theory. Methods: Data came from a subsample of EM victims (n = 191) drawn from a representative, population-based study (n = 4,156) of community-dwelling, cognitively intact older adults in New York State...
March 6, 2017: Journals of Gerontology. Series B, Psychological Sciences and Social Sciences
https://www.readbyqxmd.com/read/28329855/better-cognition-in-new-birth-cohorts-of-70-year-olds-but-greater-decline-thereafter
#8
Valgeir Thorvaldsson, Peter Karlsson, Johan Skoog, Ingmar Skoog, Boo Johansson
Objectives: To evaluate birth cohort differences in level of cognition and rate of change in old age. Methods: Data were drawn from three population-based Swedish samples including age-homogenous cohorts born 1901/02, 1906/07, and 1930, and measured on the same cognitive tests at ages 70, 75, and 79 as part of the Gerontological and Geriatric Populations Studies in Gothenburg (H70). We fitted growth curve models to the data using a Bayesian framework and derived estimates and inferences from the marginal posterior distributions...
December 9, 2016: Journals of Gerontology. Series B, Psychological Sciences and Social Sciences
https://www.readbyqxmd.com/read/28329843/consumer-decision-making-of-older-people-a-45-year-review
#9
Dominik Hettich, Stefan Hattula, Torsten Bornemann
Background and Objectives: Aging is one of the key future challenges for global life. Of particular interest is the consumption-related decision-making of older people, as its better understanding would enable the effective influence of behavior, which would help to secure the economic well-being and ensure a better quality of life for this population. This article explores the respective literature and identifies gaps for future research. Research Design and Methods: We conducted a holistic review of peer-reviewed literature that examined the decision-making of older consumers...
March 15, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329827/the-influence-of-the-physical-envirnment-on-residents-with-dementia-in-long-term-care-settings-a-review-of-the-empirical-literature
#10
Habib Chaudhury, Heather A Cooke, Heather Cowie, Leila Razaghi
Background and Objectives: The physical environment in long-term care facilities has an important role in the care of residents with dementia. This paper presents a literature review focusing on recent empirical research in this area and situates the research with therapeutic goals related to the physical environment. Research Design and Methods: A comprehensive literature search was conducted in Ageline, PsychINFO, CINAHL, Medline and Google Scholar databases to identify relevant articles...
March 10, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329825/socioeconomic-status-and-physical-functioning-a-longitudinal-study-of-older-chinese-people
#11
Lei Yang, Hanna Konttinen, Pekka Martikainen, Karri Silventoinen
Objectives: We aimed to assess the longitudinal associations of socioeconomic status and physical functioning using a large population-based survey data in China. Method: We used four waves of the Chinese Longitudinal Healthy Longevity Survey (2002-2011). Physical functioning was assessed by activities of daily living (ADL) and instrumental activities of daily living (IADL) measures. Socioeconomic status was assessed using educational attainment, occupational status, household income, financial resources, and access to health services...
February 16, 2017: Journals of Gerontology. Series B, Psychological Sciences and Social Sciences
https://www.readbyqxmd.com/read/28329823/a-typology-to-explain-changing-social-networks-post-stroke
#12
Sarah Northcott, Shashivadan P Hirani, Katerina Hilari
Background and Objectives: Social network typologies have been used to classify the general population but have not previously been applied to the stroke population. This study investigated whether social network types remain stable following a stroke, and if not, why some people shift network type. Research Design and Methods: We used a mixed methods design. Participants were recruited from two acute stroke units. They completed the Stroke Social Network Scale (SSNS) two weeks and six months post stroke and in-depth interviews 8-15 months following the stroke...
March 14, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329814/increased-engagement-with-life-differences-in-the-cognitive-physical-social-and-spiritual-activities-of-older-adult-music-listeners
#13
Christopher N Kaufmann, Lori P Montross-Thomas, Sean Griser
Purpose of the Study: Clinical studies have demonstrated the health benefits of music listening, especially among older adults; however, this connection has not yet been examined in a nationally representative population based sample. The purpose of this study was to measure the connections between health, listening to music, and engagement with life activities among older Americans. Design and Methods: We used data on 5,797 participants in both the 2012 Health and Retirement Study and 2013 Consumption and Activities Mail Survey...
January 21, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329795/construct-validation-of-wenger-s-support-network-typology
#14
Agnes Szabo, Christine Stephens, Joanne Allen, Fiona Alpass
Objectives: The study aimed to validate Wenger's empirically derived support network typology of responses to the Practitioner Assessment of Network Type (PANT) in an older New Zealander population. The configuration of network types was tested across ethnic groups and in the total sample. Method: Data (N = 872, Mage = 67 years, SDage = 1.56 years) from the 2006 wave of the New Zealand Health, Work and Retirement study were analyzed using latent profile analysis...
October 7, 2016: Journals of Gerontology. Series B, Psychological Sciences and Social Sciences
https://www.readbyqxmd.com/read/28329786/religious-affiliation-among-older-age-groups-worldwide-estimates-for-2010-and-projections-until-2050
#15
Vegard Skirbekk, Michaela Potancoková, Conrad Hackett, Marcin Stonawski
Objectives: The religious landscape of older adults around the world is changing profoundly. Yet until now, no study has chronicled these changes or compared expected aging patterns of religious groups. Differential aging among religious groups can have important economic and social consequences. This study estimates and projects the future religious composition by age at the global and regional levels. Method: This study presents estimates of age structures by religion for 2010 and projections until 2050...
November 9, 2016: Journals of Gerontology. Series B, Psychological Sciences and Social Sciences
https://www.readbyqxmd.com/read/28329784/age-related-increases-in-verbal-knowledge-are-not-associated-with-word-finding-problems-in-the-cam-can-cohort-what-you-know-won-t-hurt-you
#16
Meredith A Shafto, Lori E James, Lise Abrams, Lorraine K Tyler
Objective: We tested the claim that age-related increases in knowledge interfere with word retrieval, leading to word finding failures. We did this by relating a measure of crystallized intelligence to tip-of-the-tongue (TOT) states and picture naming accuracy. Method: Participants were from a large (N = 708), cross-sectional (aged 18-88 years), population-based sample from the Cambridge Centre for Ageing and Neuroscience cohort (Cam-CAN; www.cam-can.com). They completed (a) the Spot-the-Word Test (STW), a measure of crystallized intelligence in which participants circled the real word in word/nonword pairs, (b) a TOT-inducing task, and (c) a picture naming task...
June 30, 2016: Journals of Gerontology. Series B, Psychological Sciences and Social Sciences
https://www.readbyqxmd.com/read/28329671/trends-of-skin-cancer-incidence-following-transplantation-in-the-united-states
#17
Sarah T Arron
BackgroundSkin cancer is the most common malignancy occurring after organ transplantation. Although previous research has reported an increased risk of skin cancer in solid organ transplant recipients (OTR), no study has estimated the post-transplant population-based incidence in the US. The objective of this study is to determine the incidence and evaluate the predictors of post-transplant skin cancer, including squamous cell carcinoma (SCC), melanoma (MM), and Merkel cell carcinoma (MCC) in a cohort of US OTR receiving a primary transplant in 2003 or 2008...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329666/risks-of-different-skin-tumor-combinations-after-a-first-melanoma-squamous-cell-carcinoma-and-basal-cell-carcinoma-in-dutch-population-based-cohorts-1989-2009
#18
R J T Van der Leest, L M Hollestein, L Liu, T Nijsten, E De Vries
The aim of this study was to calculate risks of different type of subsequent skin cancers.All melanoma and squamous cell carcinoma (SCC) patients included in the national Netherlands Cancer Registry (NCR) and all basal cell carcinoma (BCC) patients included in the regional Eindhoven Cancer Registry (ECR) between 1989 and 2009 were followed until diagnosis of a subsequent different skin cancer (melanoma, SCC or BCC), date of death or end of study. Cumulative risk, Standardized Incidence Ratio (SIR) and Absolute Excess Risk (AER) of subsequent skin cancers were calculated...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329665/risk-factors-for-facial-telangiectasia
#19
Merel A Hamer, Luba M Pardo, David A Gunn, Tamar Nijsten
BackgroundTelangiectasia or red veins are one of the main features of facial skin aging. To date there are few studies investigating risk factors for telangiectasia. We investigated environmental risk factors in a population-based cohort study, using a digital continuous outcome measure.MethodsTelangiectasia were quantified digitally from standardized 3-dimensional facial photographs of 2886 North- European participants (56.8% female, median age 66.9) from the Rotterdam Study, a prospective population-based cohort study...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329659/predicting-the-risk-of-metachronous-basal-cell-carcinomas
#20
Hilde Smedinga, Joris A C Verkouteren, Ewout W Steyerberg, Albert Hofman, Tamar Nijsten, Y Vergouwe
A third of basal cell carcinoma (BCC) patients will develop metachronous BCCs, but information is limited on the frequency, timing and predictors of these subsequent BCCs. We aimed to develop a prediction model to assess the absolute risk of metachronous BCCs.We observed 14,628 participants of northwestern European ancestry from a prospective population- based cohort study (Rotterdam Study). BCCs were identified using a linkage with the Dutch Pathology Registry (PALGA). Predictors for subsequent BCCs included 14 patient, lifestyle, and tumor-specific characteristics...
September 15, 2016: Dermatology Online Journal
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