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https://www.readbyqxmd.com/read/28333553/cyclooxygenase-2-induction-by-amino-acid-deprivation-requires-p38-mitogen-activated-protein-kinase-in-human-glioma-cells
#1
Zhiwen Li, Chi-Ming Chang, Lanfang Wang, Ping Zhang, Hui-Kuo G Shu
Glioblastomas (GBMs) are malignant brain tumors that can outstrip nutrient supplies due to rapid growth. Cyclooxygenase-2 (COX-2) has been linked to GBMs and may contribute to their aggressive phenotypes. Amino acid starvation results in COX-2 mRNA and protein induction in multiple human glioma cell lines in a process requiring p38 mitogen-activated protein kinase (p38-MAPK) and the Sp1 transcription factor. Increased vascular endothelial growth factor expression results from starvation-dependent COX-2 induction...
March 23, 2017: Cancer Investigation
https://www.readbyqxmd.com/read/28330279/immobilization-of%C3%A2-bacillus-amyloliquefaciens-sp1-and-its-alkaline-protease-in-various-matrices-for-effective-hydrolysis-of-casein
#2
Shiwani Guleria, Abhishek Walia, Anjali Chauhan, C K Shirkot
An extracellular alkaline protease producing B. amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. B. amyloliquefaciens SP1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. Enzyme activity before immobilization (at 60 °C, pH 8.0 for 5 min) was 3580 µg/ml/min. The results of immobilization with various matrices revealed that 3 % calcium alginate (2829...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327666/hypoxia-induces-h19-expression-through-direct-and-indirect-hif-1%C3%AE-activity-promoting-oncogenic-effects-in-glioblastoma
#3
Weining Wu, Qi Hu, Er Nie, Tianfu Yu, Youzhi Wu, Tongle Zhi, Kuan Jiang, Feng Shen, Yingyi Wang, Junxia Zhang, Yongping You
H19 expression is elevated in many human tumors including glioblastomas, suggesting an oncogenic role for the long noncoding RNA; yet the upregulation of H19 in glioblastomas remains unclear. Here we report that hypoxia significantly stimulated H19 expression in glioblastoma cell lines, which was related to hypoxia-inducible factors 1α (Hif-1α). Hif-1α promoted H19 expression in U87 and U251 cells. Meanwhile PTEN is an advantageous factor to affect H19 expression, through attenuating Hif-1α stability. Hif-1α also positively correlates with H19 in human glioblastoma samples depending on PTEN status...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28322791/lps-depletes-phlpp-levels-in-macrophages-through-the-inhibition-of-sp1-dependent-transcriptional-regulation
#4
Neeraja P Alamuru-Yellapragada, Sanghamitra Vundyala, Soma Behera, Kishore V L Parsa
We have previously reported that bacterial endotoxin LPS attenuates expression of PHLPP, a ser/thr phosphatase, at both transcript and protein levels in different immune cells, however the underlying molecular mechanism is unknown and is of significant interest. Here, in line with the decreased transcript levels upon LPS treatment, we observed that LPS caused significant reduction in PHLPP promoter activity. We observed that SP1, a transcription factor frequently associated with inflammation, was recruited to the PHLPP promoter region...
March 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28319298/diversity-in-gold-finger-structure-elucidated-by-traveling-wave-ion-mobility-mass-spectrometry
#5
Zhifeng Du, Raphael E F de Paiva, Kristina Nelson, Nicholas P Farrell
Traveling wave ion mobility (TWIM) mass spectrometry (MS) is a powerful method for the structural and conformational analysis of proteins and peptides, enabling the differentiation of isomeric peptides (or proteins) that have the same sequence but are modified at different residues. In this study, the TWIM-MS technique was used to separate isomeric Au(I) metallopeptide ions that were formed by Zn(II) displacement from the parent zinc fingers (ZFs). The synthetic gold finger peptides were derived from the C-terminus of the HIV nucleocapsid p7 protein (NCp7-F2) and finger 3 of the Sp1 transcription factor (Sp1-F3)...
March 20, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28319127/hepatocyte-nuclear-factor-1%C3%AE-downregulates-hbv-gene-expression-and-replication-by-activating-the-nf-%C3%AE%C2%BAb-signaling-pathway
#6
Junyu Lin, Chenjian Gu, Zhongliang Shen, Yanfeng Liu, Wei Wang, Shuai Tao, Xiaoxian Cui, Jing Liu, Youhua Xie
The role of hepatocyte nuclear factor 1α (HNF1α) in the regulation of gene expression and replication of hepatitis B virus (HBV) is not fully understood. Previous reports have documented the induction of the expression of viral large surface protein (LHBs) by HNF1α through activating viral Sp1 promoter. Large amount of LHBs can block the secretion of hepatitis B surface antigen (HBsAg). Here we found that HNF1α overexpression inhibited HBV gene expression and replication in Huh7 cells, resulting in marked decreases in HBsAg, hepatitis B e antigen (HBeAg) and virion productions...
2017: PloS One
https://www.readbyqxmd.com/read/28318634/dna-methyltransferase-1-may-be-a-therapy-target-for-attenuating-diabetic-nephropathy-and-podocyte-injury
#7
Li Zhang, Qianmei Zhang, Shuangxin Liu, Yuanhan Chen, Ruizhao Li, Ting Lin, Chunping Yu, Hong Zhang, Zhongshun Huang, Xinchen Zhao, Xiaofan Tan, Zhuo Li, Zhiming Ye, Jianchao Ma, Bin Zhang, Wenjian Wang, Wei Shi, Xinling Liang
The contribution of DNA methylation to diabetic nephropathy, especially the effect on podocyte integrity, is not clarified. Here we found that albuminuria in a db/db mouse model was markedly attenuated after treatment with a DNA methylation inhibitor. This was accompanied by alleviation of glomerular hypertrophy, mesangial matrix expansion, and podocyte injury. The expression of DNA methyltransferase 1 (Dnmt1), nuclear factor Sp1, and nuclear factor kappa B (NFκB)-p65 markedly increased in podocytes in vivo and in vitro under the diabetic state...
March 15, 2017: Kidney International
https://www.readbyqxmd.com/read/28306234/whole-community-metagenomics-in-two-different-anammox-configurations-process-performance-and-community-structure
#8
Ananda S Bhattacharjee, Sha Wu, Christopher E Lawson, Mike S M Jetten, Vikram Kapoor, Jorge W Santo Domingo, Katherine D McMahon, Daniel R Noguera, Ramesh Goel
Anaerobic-ammonia-oxidation (anammox) combined with partial-nitritation (PN) is an innovative treatment process for energy-efficient nitrogen removal from wastewater. In this study, we used genome-based metagenomics to investigate the overall community structure and anammox species enriched in suspended growth (SGR) and attached growth packed bed (AGR) anammox reactors after 220 days of operation. Both reactors removed more than 85% of the total inorganic nitrogen. Metagenomic binning and phylogenetic analysis revealed that two anammox population genomes, affiliated with the genus 'Candidatus Brocadia', were differentially abundant between the SGR and AGR...
March 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28302063/epigenetic-and-genetic-alterations-and-their-influence-on-gene-regulation-in-chronic-lymphocytic-leukemia
#9
Di Huang, Ivan Ovcharenko
BACKGROUND: To understand the changes of gene regulation in carcinogenesis, we explored signals of DNA methylation - a stable epigenetic mark of gene regulatory elements - and designed a computational model to profile loss and gain of regulatory elements (REs) during carcinogenesis. We also utilized sequencing data to analyze the allele frequency of single nucleotide polymorphisms (SNPs) and detected the cancer-associated SNPs, i.e., the SNPs displaying the significant allele frequency difference between cancer and normal samples...
March 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28292968/micu1-alleviates-diabetic-cardiomyopathy-through-mitochondrial-ca-2-dependent-antioxidant-response
#10
Lele Ji, Fengzhou Liu, Zhe Jing, Qichao Huang, Ya Zhao, Haiyan Cao, Jun Li, Chun Yin, Jinliang Xing, Fei Li
Diabetic cardiomyopathy is a major cause of mortality in diabetic patients, but specific strategies for prevention or treatment of diabetic cardiomyopathy have not been clarified yet. MICU1 is a key regulator of mitochondria Ca(2+) uptake, which plays important roles in regulating mitochondrial oxidative phosphorylation and redox balance. However, to date, the significance of MICU1 in diabetic hearts has never been investigated. Here, we demonstrated that MICU1 was downregulated in db/db mouse heart, which contributes to myocardial apoptosis in diabetes...
March 14, 2017: Diabetes
https://www.readbyqxmd.com/read/28283889/cf3doda-me-induces-apoptosis-degrades-sp1-and-blocks-the-transformation-phase-of-the-blebbishield-emergency-program
#11
Rikiya Taoka, Goodwin G Jinesh, Wenrui Xue, Stephen Safe, Ashish M Kamat
Cancer stem cells are capable of undergoing cellular transformation after commencement of apoptosis through the blebbishield emergency program in a VEGF-VEGFR2-dependent manner. Development of therapeutics targeting the blebbishield emergency program would thus be important in cancer therapy. Specificity protein 1 (Sp1) orchestrates the transcription of both VEGF and VEGFR2; hence, Sp1 could act as a therapeutic target. Here, we demonstrate that CF3DODA-Me induced apoptosis, degraded Sp1, inhibited the expression of multiple drivers of the blebbishield emergency program such as VEGFR2, p70S6K, and N-Myc through activation of caspase-3, inhibited reactive oxygen species; and inhibited K-Ras activation to abolish transformation from blebbishields as well as transformation in soft agar...
March 11, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28278192/pbmc-transcriptome-profiles-identifies-potential-candidate-genes-and-functional-networks-controlling-the-innate-and-the-adaptive-immune-response-to-prrsv-vaccine-in-pietrain-pig
#12
Md Aminul Islam, Christine Große-Brinkhaus, Maren Julia Pröll, Muhammad Jasim Uddin, Sharmin Aqter Rony, Dawit Tesfaye, Ernst Tholen, Michael Hoelker, Karl Schellander, Christiane Neuhoff
The porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting swine production, health and welfare throughout the world. A synergistic action of the innate and the adaptive immune system of the host is essential for mounting a durable protective immunity through vaccination. Therefore, the current study aimed to investigate the transcriptome profiles of peripheral blood mononuclear cells (PBMCs) to characterize the innate and the adaptive immune response to PRRS Virus (PRRSV) vaccination in Pietrain pigs...
2017: PloS One
https://www.readbyqxmd.com/read/28276309/analysis-of-microrna-mrna-interactions-in-stem-cell-enriched-fraction-of-oral-squamous-cell-carcinoma
#13
Vinitha Richard, Rajesh Raju, Aswathy Mary Paul, Reshmi Girijadevi, Thankayyan Retnabai Santhoshkumar, Madhavan Radhakrishna Pillai
This study is an integrated analysis of the transcriptome profile, MicroRNA (miRNA) and their experimentally validatedmRNA targets differentially expressed in the tumorigenic stem-like fraction of oral squamous cell carcinoma (OSCC). We had previously reported the co-existence of multiple drug resistant, tumorigenic fractions termed as side population (SP1, SP2 and MP2) and a non-tumorigenic fraction, main population (MP1) in oral cancer. These fractions displayed self-renewal, regeneration potential and expressed known stemness related cell surface markers despite functional differences...
March 9, 2017: Oncology Research
https://www.readbyqxmd.com/read/28275089/the-microtubule-network-and-cell-death-are-regulated-by-a-mir-34a-stathmin-1-betaiii-tubulin-axis
#14
Nancy S Vetter, E Anders Kolb, Valerie B Sampson, Christopher Mills
MicroRNA-34a (miR-34a) is a master regulator of signaling networks that maintain normal physiology and disease and is currently in development as a miRNA-based therapy for cancer. Prior studies have reported low miR-34a expression in osteosarcoma (OS); however, the molecular mechanisms underlying miR-34a activity in OS are not well defined. Therefore, this study evaluated the role of miR-34a in regulating signal transduction pathways that influence cell death in OS. Levels of miR-34a were attenuated in human OS cells and xenografts of the Pediatric Preclinical Testing Consortium (PPTC)...
March 8, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28275049/the-antineoplastic-activity-of-bardoxolone-methyl-and-a-related-triterpenoid-in-leukemia-cells-is-due-to-reactive-oxygen-species-dependent-suppression-of-cmyc
#15
Unho Jin, Yating Cheng, Beiyan Zhou, Stephen Safe
Structurally-related pentacyclic triterpenoids methyl 2-cyano-3,12-dioxoolean-1,9-dien-28-oate [Bardoxolone-methyl (Bar-Me)] and methyl 2-trifluoromethyl-3,11-dioxoolean-1,12-dien-30-oate (CF3DODA-Me) contain 2-cyano-1-en-3-one and 2-trifluoromethyl-1-en-3-one moieties, respectively, in their A rings and differ in the position of their en-one structures in ring C. Only Bar-Me forms a Michael addition adduct with glutathione and inhibits IKKβ phosphorylation and these differences may be due to steric hinderance by the 11-keto group in CF3DODA-Me which prevents Michael addition by the conjugated en-one in the A-ring...
March 8, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28274746/transcription-factors-sf1-and-cjun-cooperate-to-activate-the-fdx1-promoter-in-ma-10-leydig-cells
#16
Pauline Roumaud, Arlette Rwigemera, Luc J Martin
The Ferredoxin 1 (FDX1) protein supports steroid biosynthesis in steroidogenic cells through electron transfer to the rate-limiting steroidogenic enzyme, CYP11A1. The latter catalyzes the conversion of cholesterol to pregnenolone through side chain cleavage inside the mitochondria. Thus far, only several transcription factors have been implicated in the regulation of mouse Fdx1 promoter activity in Leydig cells. These include the nuclear receptor SF1 and SP1. Since two conserved regulatory elements for AP1 transcription factors have been located at -764 and -617bp of the Fdx1 promoter, we hypothesized that cJUN may cooperate with other partners to regulate Fdx1 in Leydig cells...
March 6, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28270126/analysis-of-novel-sjogren-s-syndrome-autoantibodies-in-patients-with-dry-eyes
#17
Sandra Everett, Sahana Vishwanath, Vanessa Cavero, Long Shen, Lakshmanan Suresh, Kishore Malyavantham, Norah Lincoff-Cohen, Julian L Ambrus
BACKGROUND: Dry eye is a common problem in Ophthalmology and may occur for many reasons including Sjogren's syndrome (SS). Recent studies have identified autoantibodies, anti-salivary gland protein 1 (SP1), anti-carbonic anhydrase 6 (CA6) and anti-parotid secretory protein (PSP), which occur early in the course of SS. The current studies were designed to evaluate how many patients with idiopathic dry eye and no evidence of systemic diseases from a dry eye practice have these autoantibodies...
March 7, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28262799/dietary-restriction-protects-against-diethylnitrosamine-induced-hepatocellular-tumorigenesis-by-restoring-the-disturbed-gene-expression-profile
#18
Ting Duan, Wenjie Sun, Mohan Zhang, Juan Ge, Yansu He, Jun Zhang, Yifan Zheng, Wei Yang, Han-Ming Shen, Jun Yang, Xinqiang Zhu, Peilin Yu
Hepatocellular carcinoma (HCC) is one of the most lethal and prevalent malignancies, worse still, there are very limited therapeutic measures with poor clinical outcomes. Dietary restriction (DR) has been known to inhibit spontaneous and induced tumors in several species, but the mechanisms are little known. In the current study, by using a diethylnitrosamine (DEN)-induced HCC mice model, we found that DR significantly reduced the hepatic tumor number and size, delayed tumor development, suppressed proliferation and promoted apoptosis...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28257846/25-hydroxycholesterol-promotes-rankl-induced-osteoclastogenesis-through-coordinating-nfatc1-and-sp1-complex-in-the-transcription-of-mir-139-5p
#19
Lishan Zhang, Yinping Lv, Guozhe Xian, Yanliang Lin
25-hydroxycholesterol (25-HC) is implicated in many processes, including lipid metabolism and the immune response. However, the role of 25-HC in RANKL-induced osteoclastogenesis remains largely unknown. Our results showed that 25-HC inhibited miR-139-5p expression in mouse bone marrow macrophages (BMMs) cultured in receptor activator of NF-κB ligand (RANKL) and monocyte macrophage colony-stimulating factor (M-CSF). Further investigation suggested that 25-HC promoted the expression of nuclear factor of activated T cell cytoplasmic 1 (NFATc1) and Sp1, especially in the presence of RANKL and M-CSF...
April 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28256531/repression-of-human-t-lymphotropic-virus-type-1-long-terminal-repeat-sense-transcription-by-sp1-recruitment-to-novel-sp1-binding-sites
#20
Sylvain Fauquenoy, Gwenaëlle Robette, Anna Kula, Caroline Vanhulle, Sophie Bouchat, Nadège Delacourt, Anthony Rodari, Céline Marban, Christian Schwartz, Arsène Burny, Olivier Rohr, Benoit Van Driessche, Carine Van Lint
Human T-lymphotropic Virus type 1 (HTLV-1) infection is characterized by viral latency in the majority of infected cells and by the absence of viremia. These features are thought to be due to the repression of viral sense transcription in vivo. Here, our in silico analysis of the HTLV-1 Long Terminal Repeat (LTR) promoter nucleotide sequence revealed, in addition to the four Sp1 binding sites previously identified, the presence of two additional potential Sp1 sites within the R region. We demonstrated that the Sp1 and Sp3 transcription factors bound in vitro to these two sites and compared the binding affinity for Sp1 of all six different HTLV-1 Sp1 sites...
March 3, 2017: Scientific Reports
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