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https://www.readbyqxmd.com/read/27925196/sirt1-sirt3-axis-regulates-cellular-response-to-oxidative-stress-and-etoposide
#1
Ilaria Carnevale, Laura Pellegrini, Patrizia D'Aquila, Serena Saladini, Emanuela Lococo, Lucia Polletta, Enza Vernucci, Eleonora Foglio, Stefano Coppola, Luigi Sansone, Giuseppe Passarino, Dina Bellizzi, Matteo A Russo, Massimo Fini, Marco Tafani
Sirtuins are conserved NAD+-dependent deacylases. SIRT1 is a nuclear and cytoplasmic sirtuin involved in the control of histones a transcription factors function. SIRT3 is a mitochondrial protein, which regulates mitochondrial function. Although both SIRT1 and SIRT3 have been implicated in resistance to cellular stress, the link between these two sirtuins has not been studied so far. Here we aimed to unravel: i) the role of SIRT1-SIRT3 axis for cellular response to oxidative stress and DNA damage; ii) how mammalian cells modulate such SIRT1-SIRT3 axis and which mechanisms are involved...
December 7, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27918959/specificity-protein-1-zinc-finger-protein-179-pathway-is-involved-in-the-attenuation-of-oxidative-stress-following-brain-injury
#2
Jian-Ying Chuang, Tzu-Jen Kao, Shu-Hui Lin, An-Chih Wu, Pin-Tse Lee, Tsung-Ping Su, Shiu-Hwa Yeh, Yi-Chao Lee, Chung-Che Wu, Wen-Chang Chang
After sudden traumatic brain injuries, secondary injuries may occur during the following days or weeks, which leads to the accumulation of reactive oxygen species (ROS). Since ROS exacerbate brain damage, it is important to protect neurons against their activity. Zinc finger protein 179 (Znf179) was shown to act as a neuroprotective factor, but the regulation of gene expression under oxidative stress remains unknown. In this study, we demonstrated an increase in Znf179 protein levels in both in vitro model of hydrogen peroxide (H2O2)-induced ROS accumulation and animal models of traumatic brain injury...
November 29, 2016: Redox Biology
https://www.readbyqxmd.com/read/27915032/reelin-expression-is-up-regulated-in-mice-colon-in-response-to-acute-colitis-and-provides-resistance-against-colitis
#3
Ana E Carvajal, María D Vázquez-Carretero, Pablo García-Miranda, María J Peral, María L Calonge, Anunciación A Ilundain
Reelin is an extracellular matrix protein first known for its key role in neuronal migration. Studies in rodent small intestine suggested that reelin protects the organism from intestinal pathology. Here we determined in mice colon, by real time-PCR and immunological assays, the expression of the reelin signalling system; its response to dextran sulphate sodium (DSS) and the response of wild-type and reeler mice to DSS. DNA methylation was determined by bisulfite modification and sequencing of genomic DNA. In the colon mucosa reelin expression is restricted to the myofibroblasts, whereas both epithelial cells and myofibroblasts express reelin receptors (ApoER2 and VLDLR) and its effector protein Dab1...
November 30, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27906112/chromatin-wide-and-transcriptome-profiling-integration-uncovers-p38%C3%AE-mapk-as-a-global-regulator-of-skeletal-muscle-differentiation
#4
Jessica Segalés, Abul B M M K Islam, Roshan Kumar, Qi-Cai Liu, Pedro Sousa-Victor, F Jeffrey Dilworth, Esteban Ballestar, Eusebio Perdiguero, Pura Muñoz-Cánoves
BACKGROUND: Extracellular stimuli induce gene expression responses through intracellular signaling mediators. The p38 signaling pathway is a paradigm of the mitogen-activated protein kinase (MAPK) family that, although originally identified as stress-response mediator, contributes to establishing stem cell differentiation fates. p38α is central for induction of the differentiation fate of the skeletal muscle stem cells (satellite cells) through not fully characterized mechanisms. METHODS: To investigate the global gene transcription program regulated by p38α during satellite cell differentiation (myogenesis), and to specifically address whether this regulation occurs through direct action of p38α on gene promoters, we performed a combination of microarray gene expression and genome-wide binding analyses...
March 15, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27906060/discrete-adipose-derived-stem-cell-subpopulations-may-display-differential-functionality-after-in-vitro-expansion-despite-convergence-to-a-common-phenotype-distribution
#5
Frederik Mølgaard Nielsen, Simone Elkjær Riis, Jens Isak Andersen, Raphaëlle Lesage, Trine Fink, Cristian Pablo Pennisi, Vladimir Zachar
BACKGROUND: Complex immunophenotypic repertoires defining discrete adipose-derived stem cell (ASC) subpopulations may hold a key toward identifying predictors of clinical utility. To this end, we sorted out of the freshly established ASCs four subpopulations (SPs) according to a specific pattern of co-expression of six surface markers, the CD34, CD73, CD90, CD105, CD146, and CD271, using polychromatic flow cytometry. METHOD: Using flow cytometry-associated cell sorting and analysis, gating parameters were set to select for a CD73(+)CD90(+)CD105(+) phenotype plus one of the four following combinations, CD34(-)CD146(-)CD271(-) (SP1), CD34(-)CD146(+)CD271(-) (SP2), CD34(+)CD146(+)CD271(-) (SP3), and CD34(-)CD146(+)CD271(+) (SP4)...
December 1, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27904693/berberine-upregulates-mir-22-3p-to-suppress-hepatocellular-carcinoma-cell-proliferation-by-targeting-sp1
#6
Jie Chen, Fei-Xiang Wu, Hong-Lin Luo, Jun-Jie Liu, Tao Luo, Tao Bai, Le-Qun Li, Xiao-Hui Fan
MicroRNA-22-3p (miR-22-3p) is downregulated in hepatocellular carcinoma (HCC), which contributes to the development and progression of HCC. In this study, berberine treatment upregulated miR-22-3p expression in HepG2 cells. Therefore, we investigated whether berberine suppresses the proliferation of HCC cells and explored the underlying mechanism. The HCC HepG2 cell line was treated with a gradient of berberine concentrations (0-300 μM) for 48 h, and 100 μM berberine inhibited cell growth at 24 h. The HepG2 cells were then incubated with 100 μM berberine for 0-48 h, and after treatment for 24 h, berberine markedly suppressed HepG2 cell growth and significantly upregulated miR-22-3p expression...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27901471/esculetin-exerts-anti-proliferative-effects-against-non-small-cell-lung-carcinoma-by-suppressing-specificity-protein-1-in-vitro
#7
Ra H Lee, Young-Joo Jeon, Jin H Cho, Jeong-Yun Jang, Il-Keun Kong, Seok-Ho Kim, MinSeok S Kim, Hak-Jae Chung, Keon B Oh, Seon-Min Park, Jae-Cheon Shin, Jae-Min Seo, Sungho Ko, Jung-Hyun Shim, Jung-Il Chae
Esculetin, a coumarin derivative, is a phenolic compound isolated from Artemisia capillaris, Citrus limonia, and Euphorbia lathyris. Although it has been reported to have anti-inflammatory, anti-oxidant, and anti-proliferative activities in several human cancers, its anti-proliferative activity against non-small-cell lung carcinoma (NSCLC) and the molecular mechanisms involved have not been adequately elucidated. In this study, we used two NSCLC cell lines (NCI-H358 and NCI-H1299) to investigate the anti-proliferative activity and apoptotic effect of esculetin...
November 30, 2016: General Physiology and Biophysics
https://www.readbyqxmd.com/read/27899892/age-dependent-hepatic-udp-glucuronosyltransferase-gene-expression-and-activity-in-children
#8
Elizabeth Neumann, Huma Mehboob, Jacqueline Ramírez, Snezana Mirkov, Min Zhang, Wanqing Liu
UDP-glucuronosyltransferases (UGTs) are important phase II drug metabolism enzymes. The aim of this study was to explore the relationship between age and changes in mRNA expression and activity of major human hepatic UGTs, as well as to understand the potential regulatory mechanism underlying this relationship. Using previously generated data, we investigated age-dependent mRNA expression levels of 11 hepatic UGTs (UGT1A1, UGT1A3, UGT1A4, UGT1A5, UGT1A6, UGT1A9, UGT2B4, UGT2B7, UGT2B10, UGT2B15, and UGT2B17) and 16 transcription factors (AHR, AR, CAR, ESR2, FXR, GCCR, HNF1a, HNF3a, HNF3b, HNF4a, PPARA, PPARG, PPARGC, PXR, SP1, and STAT3) in liver tissue of donors (n = 38) ranging from 0 to 25 years of age...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27893830/mechanistic-studies-and-modeling-reveal-the-origin-of-differential-inhibition-of-gag-polymorphic-viruses-by-hiv-1-maturation-inhibitors
#9
Zeyu Lin, Joseph Cantone, Hao Lu, Beata Nowicka-Sans, Tricia Protack, Tian Yuan, Hong Yang, Zheng Liu, Dieter Drexler, Alicia Regueiro-Ren, Nicholas A Meanwell, Mark Cockett, Mark Krystal, Max Lataillade, Ira B Dicker
HIV-1 maturation inhibitors (MIs) disrupt the final step in the HIV-1 protease-mediated cleavage of the Gag polyprotein between capsid p24 capsid (CA) and spacer peptide 1 (SP1), leading to the production of infectious virus. BMS-955176 is a second generation MI with improved antiviral activity toward polymorphic Gag variants compared to a first generation MI bevirimat (BVM). The underlying mechanistic reasons for the differences in polymorphic coverage were studied using antiviral assays, an LC/MS assay that quantitatively characterizes CA/SP1 cleavage kinetics of virus like particles (VLPs) and a radiolabel binding assay to determine VLP/MI affinities and dissociation kinetics...
November 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27889927/sp2-is-the-only-glutamine-rich-specificity-protein-with-minor-impact-on-development-and-differentiation-in-myelinating-glia
#10
Amélie Wegener, Melanie Küspert, Elisabeth Sock, Sjaak Philipsen, Guntram Suske, Michael Wegner
Oligodendrocytes and Schwann cells are the myelinating glia of the vertebrate nervous system and by generation of myelin sheaths allow rapid saltatory conduction. Previous in vitro work had pointed to a role of the C2H2 zinc finger Specificity proteins Sp1 and Sp3 as major regulators of glial differentiation and myelination. Here, we asked whether such a role is also evident in vivo using mice with specific deletions of Sp1 or Sp3 in myelinating glia. We also studied glia-specific conditional Sp2- and constitutive Sp4-deficient mice to include all related glutamine-rich Sp factors into our analysis...
November 27, 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27889799/a-polymorphism-in-the-promoter-region-of-pd-l1-serves-as-a-binding-site-for-sp1-and-is-associated-with-pd-l1-overexpression-and-increased-occurrence-of-gastric-cancer
#11
Li-Hua Tao, Xin-Ru Zhou, Fu-Chao Li, Qi Chen, Fan-Yi Meng, Yong Mao, Rui Li, Dong Hua, Hong-Jian Zhang, Wei-Peng Wang, Wei-Chang Chen
PD-L1 is a member of the B7 family co-inhibitory molecules and plays a critical role in tumor immune escape. In this study, we found a polymorphism rs10815225 in the PD-L1 promoter region was significantly associated with the occurrence of gastric cancer. The GG homozygous frequency was higher in the cancer patients than that in the precancerous lesions, which was higher than that in the health controls. This polymorphism locates in the binding-site of Sp1 transcription factor (SP1). The expression level of PD-L1 mRNA in the GG homozygous cancer patients was apparently higher than that in the GC heterozygotes...
November 26, 2016: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/27889233/icaritin-induces-mitochondrial-apoptosis-by-up-regulating-mir-124-in-human-oral-squamous-cell-carcinoma-cells
#12
Limin Jin, Jinhong Miao, Yanjin Liu, Xingdan Li, Yaqiong Jie, Qianyun Niu, Xinguang Han
AIM OF THE STUDY: The present study is aimed to investigate the apoptosis-inducing effect of icaritin in human oral squamous cell carcinoma (OSCC) cells and the associated mechanisms. MATERIALS AND METHODS: KB and SCC9 cell lines were used as model cell lines. Effect of icaritin on apoptosis was analyzed by flow cytometry. The effect of icaritin on mitochondrial apoptotic pathway was demonstrated by loss of mitochondrial membrane potential and release of cytocrome C from mitochondria...
November 23, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27881869/netrin-1-induced-stem-cell-bioactivity-contributes-to-the-regeneration-of-injured-tissues-via-the-lipid-raft-dependent-integrin-%C3%AE-6%C3%AE-4-signaling-pathway
#13
Soo Sang Lee, Sei-Jung Lee, Sang Hun Lee, Jung Min Ryu, Hyeon Su Lim, Jun Sung Kim, Eun Ju Song, Young Hyun Jung, Hyun Jik Lee, Chung Hun Kim, Ho Jae Han
Netrin-1 (Ntn-1) is a multifunctional neuronal signaling molecule; however, its physiological significance, which improves the tissue-regeneration capacity of stem cells, has not been characterized. In the present study, we investigate the mechanism by which Ntn-1 promotes the proliferation of hUCB-MSCs with regard to the regeneration of injured tissues. We found that Ntn-1 induces the proliferation of hUCB-MSCs mainly via Inα6β4 coupled with c-Src. Ntn-1 induced the recruitment of NADPH oxidases and Rac1 into membrane lipid rafts to facilitate ROS production...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27881653/microrna-373-facilitated-the-replication-of-porcine-reproductive-and-respiratory-syndrome-virus-prrsv-by-its-negative-regulation-of-type-i-interferon-induction
#14
Jing Chen, Xibao Shi, Xiaozhuan Zhang, Aiping Wang, Li Wang, Yanyan Yang, Ruiguang Deng, Gai-Ping Zhang
: microRNAs (miRNAs) played an important role in the regulation of immune response. Previous studies have indicated that dysregulating the miRNAs may lead to the immunosuppression of porcine reproductive and respiratory syndrome virus (PRRSV). However, it's not clear about how PRRSV regulated the expression of host miRNA which may make the immune escape or promote the replication of the virus. The present work suggested that PRRSV up-regulated the expression of miR-373 through elevating the expression of specificity protein 1 (Sp1) in MARC-145 cells...
November 23, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27878251/glyceraldehyde-3-phosphate-dehydrogenase-promotes-cancer-growth-and-metastasis-through-upregulation-of-snail-expression
#15
Kaiyan Liu, Zhenjie Tang, Amin Huang, Ping Chen, Panpan Liu, Jing Yang, Wenhua Lu, Jianwei Liao, Yicheng Sun, Shijun Wen, Yumin Hu, Peng Huang
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays an important role in multiple cellular functions including metabolism and gene transcription. Our previous study showed that GAPDH expression was elevated in colon cancer and further upregulated in liver metastatic tissues, suggesting a possilbe role of GAPDH in promoting cancer metastasis. The present study was designed to investigate the underlying mechanism, using multiple experimental approaches including genetic silencing of GAPDH expression by short hairpin RNA (shRNA) and biochemcial/molecular analyses of the key events involved in glycolytic metabolism and epithelial-mesenchymal transition (EMT)...
November 18, 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27878236/pyropia-yezoensis-peptide-promotes-collagen-synthesis-by-activating-the-tgf-%C3%AE-smad-signaling-pathway-in-the-human-dermal-fibroblast-cell-line-hs27
#16
Cho-Rong Kim, Young-Min Kim, Min-Kyeong Lee, In-Hye Kim, Youn-Hee Choi, Taek-Jeong Nam
Pyropia yezoensis (P. yezoensis) is a marine algae that exhibits antioxidant, anti-inflammatory, antitumor and anti-aging activities. In this study, we investigated the effects of the P. yezoensis peptide, PYP1‑5, on collagen synthesis in the human dermal fibroblast cell line Hs27. Skin aging is related to reduced collagen production and the activities of multiple enzymes, including matrix metalloproteinases (MMPs), which degrade collagen structure in the dermis, and tissue inhibitor of tissue inhibitor of metalloproteinases (TIMPs), which inhibit the action of MMPs...
November 18, 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27875298/benzylisothiocyanate-bitc-induces-ros-dependent-repression-of-stat3-by-downregulation-of-specificity-proteins-in-pancreatic-cancer
#17
Ravi Kasiappan, Indira Jutooru, Keshav Karki, Erik Hedrick, Stephen H Safe
The antineoplastic agent benzylisothiocyanate (BITC) acts by targeting multiple pro-oncogenic pathways/genes, including signal transducer and activator of transcription 3 (STAT3); however, the mechanism of action is not well known. As previously reported, BITC induced ROS in Panc1, MiaPaCa2 and L3.6pL pancreatic cancer cells and this was accompanied by induction of apoptosis and inhibition of cell growth and migration, and these responses were attenuated in cells cotreated with BITC plus glutathione (GSH). BITC also decreased expression of Sp1, Sp3 and Sp4 transcription factors (TFs) and several pro-oncogenic Sp-regulated genes including STAT3 and phospho-STAT3 (pSTAT3), and GSH attenuated these responses...
November 15, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27875294/spontaneous-glutamatergic-synaptic-activity-regulates-constitutive-cox-2-expression-in-neurons-opposing-roles-for-the-transcription-factors-creb-and-sp1
#18
Sandra J Hewett, Jingxue Shi, Yifan Gong, Krishnan Dhandapani, Carol Pilbeam, James A Hewett
Burgeoning evidence supports a role for cyclooxygenase metabolites in regulating membrane excitability in various forms of synaptic plasticity. Two cyclooxygenases, COX-1 and COX-2, catalyze the initial step in the metabolism of arachidonic acid to prostaglandins. COX-2 is generally considered inducible, but in glutamatergic neurons in some brain regions, including the cerebral cortex, it is constitutively expressed. However, the transcriptional mechanisms by which this occurs have not been elucidated. Here, we used quantitative PCR and also analyzed reporter gene expression in a mouse line carrying a construct consisting of a portion of the proximal promoter region of the mouse COX-2 gene upstream of luciferase cDNA to characterize COX-2 basal transcriptional regulation in cortical neurons...
November 14, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27874288/n-heterocyclic-carbene-complexes-of-silver-and-gold-as-novel-tools-against-breast-cancer-progression
#19
Carmela Saturnino, Ines Barone, Domenico Iacopetta, Annaluisa Mariconda, Maria Stefania Sinicropi, Camillo Rosano, Antonella Campana, Stefania Catalano, Pasquale Longo, Sebastiano Andò
AIM: Metal carbenic complexes have received considerable attention in both the catalysis and biological fields for their potential applications in cancer and antimicrobial therapies. RESULTS: A small series of new silver and gold N-heterocyclic carbene complexes has been designed and synthesized. Among the tested complexes, one compound was particularly active in inhibiting anchorage-dependent and -independent breast cancer proliferation, and inducing cell apoptosis via a mitochondria-related process...
November 22, 2016: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/27848899/computational-modeling-approach-in-probing-effects-of-cytosine-methylation-on-the-transcription-factor-binding-to-dna
#20
John Tenayuca, Kimberley Cousins, Shumei Yang, Lubo Zhang
Cytosine methylation at CpG dinucleotides is a chief mechanism in epigenetic modification of gene expression patterns. Previous studies demonstrated that increased CpG methylation of Sp1 sites at -268 and -346 of protein kinase C  promoter repressed the gene expression. The present study investigated the impact of CpG methylation on the Sp1 binding via molecular modeling and electrophoretic mobility shift assay. Each of the Sp1 sites contain two CpGs. Methylation of either CpG lowered the binding affinity of Sp1, whereas methylation of both CpGs produced a greater decrease in the binding affinity...
November 16, 2016: Current Topics in Medicinal Chemistry
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