Cian Schmitt-Ulms, Alisan Kayabolen, Marcos Manero-Carranza, Nathan Zhou, Keira Donnelly, Sabrina Pia Nuccio, Kazuki Kato, Hiroshi Nishimasu, Jonathan S Gootenberg, Omar O Abudayyeh
RNA editing offers the opportunity to introduce either stable or transient modifications to nucleic acid sequence without permanent off-target effects, but installation of arbitrary edits into the transcriptome is currently infeasible. Here, we describe Programmable RNA Editing & Cleavage for Insertion, Substitution, and Erasure (PRECISE), a versatile RNA editing method for writing RNA of arbitrary length and sequence into existing pre-mRNAs via 5' or 3' trans-splicing. In trans-splicing, an exogenous template is introduced to compete with the endogenous pre-mRNA, allowing for replacement of upstream or downstream exon sequence...
February 1, 2024: bioRxiv