Nuwan C Hettige, Peter Fleming, Amelia Semenak, Xin Zhang, Huashan Peng, Marc-Daniel Hagel, Jean-François Théroux, Ying Zhang, Anjie Ni, Malvin Jefri, Lilit Antonyan, Shaima Alsuwaidi, Andreas Schuppert, Patrick S Stumpf, Carl Ernst
FOXG1 is a critical transcription factor in human brain where loss-of-function mutations cause a severe neurodevelopmental disorder, while increased FOXG1 expression is frequently observed in glioblastoma. FOXG1 is an inhibitor of cell patterning and an activator of cell proliferation in chordate model organisms but different mechanisms have been proposed as to how this occurs. To identify genomic targets of FOXG1 in human neural progenitor cells (NPCs), we engineered a cleavable reporter construct in endogenous FOXG1 and performed chromatin immunoprecipitation (ChIP) sequencing...
May 22, 2023: Human Molecular Genetics