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Staphylococcus aureus

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https://www.readbyqxmd.com/read/28941388/high-frequency-of-s-aureus-mssa-mrsa-in-children-under-1-year-old-with-skin-and-soft-tissue-infections
#1
Lorena Salazar, Judy Natalia Jimenez Quiceno
OBJECTIVE: Staphylococcus aureus is responsible for a large number of infections in pediatric population; however, information about the behavior of such infections in this population is limited. The aim of the study was to describe the clinical, epidemiological, and molecular characteristics of infections caused by methicillin-susceptible and resistant S. aureus (MSSA-MRSA) in a pediatric population. METHOD: A cross-sectional descriptive study in patients from birth to 14 years of age from three high-complexity institutions was conducted (2008-2010)...
September 20, 2017: Jornal de Pediatria
https://www.readbyqxmd.com/read/28938539/dissemination-of-macrolides-fusidic-acid-and-mupirocin-resistance-among-staphylococcus-aureus-clinical-isolates
#2
Xingmei Liu, Shanshan Deng, Jinwei Huang, Yaling Huang, Yu Zhang, Qin Yan, Yanhong Wang, Yanyue Li, Chengfu Sun, Xu Jia
As an increasingly common cause of skin infections worldwide, the prevalence of antibiotic-resistant Staphylococcus aureus (S. aureus) across China has not been well documented. This literature aims to study the resistance profile to commonly used antibiotics, including macrolides, fusidic acid (FA) and mupirocin, and its relationship to the genetic typing in 34 S. aureus strains, including 6 methicillin-resistant S. aureus (MRSA), isolated from a Chinese hospital. The MIC results showed 27 (79.4%), 1 (2.9%) and 6 (17...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938380/bacterial-keratitis-in-toronto-a-16-year-review-of-the-microorganisms-isolated-and-the-resistance-patterns-observed
#3
Alex L C Tam, Elie Côté, Mario Saldanha, Alejandro Lichtinger, Allan R Slomovic
PURPOSE: To review the incidence, distribution, current trends, and resistance patterns of bacterial keratitis isolates in Toronto over the past 16 years. METHODS: Microbiology records of suspected bacterial keratitis that underwent a diagnostic corneal scraping and cultures from January 1, 2000, through December 31, 2015, were retrospectively reviewed. The distribution of the main isolated pathogens and in vitro laboratory minimum inhibitory concentration testing results were used to identify resistance patterns...
September 21, 2017: Cornea
https://www.readbyqxmd.com/read/28938203/green-synthesis-biological-and-spectroscopic-study-on-the-interaction-of-multi-component-mannich-bases-of-imidazo-2-1-b-benzothiazoles-with-human-serum-albumin
#4
Swetha K Maddili, Leela Prasad Yandrati, Shakuntala Siddam, Vijaya Kumar Kannekanti, Himabindu Gandham
A series of Mannich bases of imidazo[2, l-b]benzothiazoles were prepared through one-pot multi-component reaction in the presence of water as an eco-friendly solvent. All the synthesized compounds were confirmed from IR, (1)HNMR, (13)CNMR, and Mass spectroscopy. Evaluation of in vitro anti-inflammatory and anti-microbial activities of all the synthesized derivatives was further accomplished. These results clearly displayed that compound 6d exhibited outstanding anti-inflammatory activity with a percentage inhibition of 70...
August 19, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28937780/-isolated-infectious-endocarditis-of-the-pulmonary-valve-a%C3%A2-case-report
#5
Jan Hrudka, Monika Grussmannová, Václav Mandys
Isolated infectious endocarditis of the pulmonary valve is a rare condition and represents 1,5-2% of all cases of infectious endocarditis. We present a case of a 37year-old woman without any relevant medical history. The woman was hospitalized with hallmarks of severe sepsis and bilateral pneumonia; she died several hours after admission with progressive multiorgan failure and disseminated intravascular coagulopathy. Microbiologic examination approved Staphylococcus aureus as the etiological agent. The autopsy showed isolated endocarditis of the pulmonary valve, without any known predisposing factor...
2017: Ceskoslovenská Patologie
https://www.readbyqxmd.com/read/28936955/-analysis-of-characteristics-of-bacteria-in-respiratory-tract-infection-in-2013-2016-in-heibei-3a-hospital-a-single-center-report-of-7-497-patients
#6
Lili Hou, Lili Liu, Ping Dang, Guannan Kang, Qinfeng Zhang, Dongling Li
OBJECTIVE: To analyze the changes and characteristics of respiratory tract bacteria in Hebei 3A Hospital, and to provide new rationale for clinical diagnosis and treatment. METHODS: A single-center retrospective analysis was conducted. 7 497 patients with respiratory tract infection admitted to Hebei Chest Hospital from January 2013 to December 2016 were enrolled. Deep sputum was collected, and the bacterial cultures and susceptibility analysis was conducted in sputum and upper respiratory secretions were collected by fiberoptic bronchoscopy...
September 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28936671/use-of-the-cas9-orthologs-from-streptococcus-thermophilus-and-staphylococcus-aureus-for-non-homologous-end-joining-mediated-site-specific-mutagenesis-in-arabidopsis-thaliana
#7
Jeannette Steinert, Carla Schmidt, Holger Puchta
Since the discovery of the CRISPR/Cas system and its in vivo application for site-specific targeted mutagenesis, this technique is wildly used in a great variety of organisms, such as many plant species. Commonly used for this application is the Cas9 enzyme from Streptococcus pyogenes. Here, we describe the application of two Cas9 orthologs from Streptococcus thermophilus and Staphylococcus aureus for targeted non-homologous end-joining mediated mutagenesis in Arabidopsis thaliana. With both orthologs, we could show efficient inheritance of the induced mutations...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936051/bacterial-contamination-of-multi-dose-eye-drops-at-ophthalmology-department-university-of-gondar-northwest-ethiopia
#8
Asegedech Tsegaw, Asamere Tsegaw, Tefera Abula, Yared Assefa
PURPOSE: Ophthalmic solutions used for both diagnostic and therapeutic purposes were found to be contaminated with bacteria pathogens and caused serious ocular infections such as keratitis and endophthalmitis. The objective was to assess the magnitude and pattern of bacterial contamination of multi-dose ophthalmic medications and investigate the drug susceptibility pattern of the isolates in the Department of Ophthalmology at Gondar University Teaching Hospital. METHODS: A total of 100 ophthalmic medications in-use by patients and eye-care workers have been taken and cultured for potential bacterial contamination in the Microbiology Department after 1 week and >1 week of use...
April 2017: Middle East African Journal of Ophthalmology
https://www.readbyqxmd.com/read/28935900/antibacterial-and-immunomodulatory-activities-of-insect-defensins-dlp2-and-dlp4-against-multidrug-resistant-staphylococcus-aureus
#9
Zhanzhan Li, Ruoyu Mao, Da Teng, Ya Hao, Huixian Chen, Xiumin Wang, Xiao Wang, Na Yang, Jianhua Wang
Methicillin-resistant Staphylococcus aureus (MRSA), are the most frequent cause of sepsis, which urgently demanding new drugs for treating infection. Two homologous insect CSαβ peptides-DLP2 and DLP4 from Hermetia illucens were firstly expressed in Pichia pastoris, with the yields of 873.5 and 801.3 mg/l, respectively. DLP2 and DLP4 displayed potent antimicrobial activity against Gram-positive bacteria especially MRSA and had greater potency, faster killing, and a longer postantibiotic effect than vancomycin...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28935553/prevalence-risk-factors-and-antimicrobial-susceptibility-test-of-staphylococcus-aureus-in-bovine-cross-breed-mastitic-milk-in-and-around-asella-town-oromia-regional-state-southern-ethiopia
#10
Befikadu Seyoum, Hailemariam Kefyalew, Birhanu Abera, Nejash Abdela
A cross sectional study was undertaken from November 2016 to March 2017 in and around Asella town, Oromia regional state, southern Ethiopia, to determine the prevalence, associated risk factors and antimicrobial susceptibility of Staphylococcus aureus, in Bovine cross breed mastitis milk. A total of 384 lactating dairy cows were screened for mastitis based on clinical examinations and California mastitis test (CMT). Out of 230 lactating crossbred cows with either clinical or subclinical mastitis examined for the involvement of Staphylococcus aureus...
September 18, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28935494/-an-atypical-case-of-necrotizing-staphylococcal-pneumonia
#11
E Bertreau, S Grard, T Baudry, N Freymond
INTRODUCTION: Some strains of Staphylococcus aureus produce a toxin known as Panton-Valentine leukocidin. These strains notably cause a necrotizing pneumonia which is associated with a high mortality. OBSERVATION: A 70-year-old woman presented with sub-acute onset dyspnea, low-grade fever, and hemoptysis after a trip to Dubai and New Zealand. Computed tomography showed bilateral necrotizing pneumonia, suggesting the diagnosis of pneumonia caused by S. aureus producing Panton-Valentine toxin...
September 18, 2017: Revue des Maladies Respiratoires
https://www.readbyqxmd.com/read/28935372/structural-insights-into-the-dimer-tetramer-transition-of-fabi-from-bacillus-anthracis
#12
Hyun Tae Kim, Sulhee Kim, Byeong Kwan Na, Jiwoung Chung, Eunha Hwang, Kwang Yeon Hwang
Enoyl-ACP reductase (ENR, also known as FabI) has received considerable interest as an anti-bacterial target due to its essentiality in fatty acid synthesis. All the FabI structures reported to date, regardless of the organism, are composed of homo-tetramers, except for two structures: Bacillus cereus and Staphylococcus aureus FabI (bcFabI and saFabI, respectively), which have been reported as dimers. However, the reason for the existence of the dimeric form in these organisms and the biological meaning of dimeric and tetrameric forms of FabI are ambiguous...
September 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28935221/thermal-stability-of-mafenide-and-amphotericin-b-topical-solution
#13
Julie A Rizzo, Angela K Martini, Kaitlin A Pruskowski, Matthew P Rowan, Krista L Niece, Kevin S Akers
OBJECTIVE: Fungal infections remain a major cause of mortality in the burned population. Mafenide acetate/amphotericin B solution (SMAT) has been used topically for prophylaxis and treatment of these infections. Current manufacturer guidelines only guarantee the stability of mafenide solution and amphotericin B at room temperature. Additionally, the recommended maximum storage time for mafenide solution is 48h, leading to significant financial and material loss when unused solutions are discarded...
September 18, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28935203/hr-lc-ms-based-analysis-of-two-antibacterial-metabolites-from-a-marine-sponge-symbiont-streptomyces-pharmamarensis-icn40
#14
Francis-Joseph Rosemary Sharmila Joseph, Appadurai Muthamil Iniyan, Samuel Gnana Prakash Vincent
On the effort to screen antibiotics against Methicillin resistant Staphylococcus aureus (MRSA), an actinomycete strain which can produce bactericidal compound was isolated from a marine sponge of Kanyakumari Coast, India. Two anti-MRSA compounds (PVI401 and PVI402) were isolated from the fermentation plates of Streptomyces pharmamarensis ICN40. TLC bioautography analysis yielded two active spots with Rf value of 0.75 (PVI401) and 0.8 (PVI402) from the crude extract. Both the compounds were characterized by HR-LC-MS analysis...
September 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28935003/spontaneous-infection-caused-by-streptococcus-agalactiae-in-kk-a-y-mice
#15
Tetsufumi Koga, Wataru Aoki, Miki Fujii, Kiyoshi Satou, Yoshinori Ikeda
During 2006 through 2012, spontaneous group B Streptococcus infections were reported in 22 female KK-Ay mice, an animal model of type 2 diabetes. The affected mice were 5 to 27 wk old, and the cases involved various body sites, including cases of submandibular, caudal, and lumbar abscesses (n =18) or led to torticollis (n = 2), hydrocephalus (n = 1), or moribund clinical signs (n = 1). At necropsy, the mouse with hydrocephalus also demonstrated retained exudate in the uterus, and the moribund mouse showed renal inflammation...
October 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28934980/in-vitro-antibiotic-susceptibility-and-biofilm-production-of-staphylococcus-aureus-isolates-recovered-from-bovine-intramammary-infections-that-persisted-or-not-following-extended-therapies-with-cephapirin-pirlimycin-or-ceftiofur
#16
Céline Ster, Valérie Lebeau, Julia Leclerc, Alexandre Fugère, Koui A Veh, Jean-Philippe Roy, François Malouin
Staphylococcus aureus intramammary infections (IMIs) have low cure rates using standard antibiotic treatment and increasing the duration of treatment usually improves therapeutic success. Chronic IMIs are thought to be caused by bacteria presenting a specific virulence phenotype that includes the capacity to produce greater amounts of biofilm. In this study, antibiotic susceptibility and biofilm production by S. aureus isolates recovered from IMIs that were cured or not following an extended therapy with cephapirin, pirlimycin or ceftiofur for 5, 8 and 8 days, respectively, were compared...
September 21, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28934797/percutaneous-endoscopic-debridement-and-drainage-with-four-different-approach-methods-for-the-treatment-of-spinal-infection
#17
Eun-Ji Choi, Su-Young Kim, Hyuck-Goo Kim, Hong-Sik Shon, Tae-Kyun Kim, Kyung-Hoon Kim
BACKGROUND: The incidence of spinal infection seems to be increasing in recent years. Percutaneous endoscopic debridement and drainage (PEDD) has become an effective alternative to extensive open surgery. OBJECTIVE: This study reviewed the charter of patients who received PEDD using 4 different approach methods to evaluate the clinical results. STUDY DESIGN: An Institutional Review Board (IRB)-approved retrospective chart review. SETTING: University hospital inpatient referred to our pain clinic...
September 2017: Pain Physician
https://www.readbyqxmd.com/read/28934660/antimicrobial-activity-of-some-natural-extracts-encapsulated-within-silica-matrices
#18
Alexandra Duprates Steiner, Alvaro Vargas, Nei Fronza, Adriano Brandelli, João Henrique Zimnoch Dos Santos
Natural extracts (anthocyanins, tannin, anatto, curcuma and olive leaf extracts) were encapsulated within a silica network by acid or base-catalyzed sol-gel methods. The nominal encapsulated contents were between 2.5 and 50wt.-%. The resulting materials were characterized by Fourier transform infrared spectroscopy, diffuse reflectance in the UV region, nitrogen adsorption/desorption porosimetry and small angle X-ray scattering. Encapsulated anthocyanin and tannin afforded inhibition zones between 9-21mm towards Staphylococcus aureus, Escherichia coli, Bacillus cereus, Candida sp...
September 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28934372/antibacterial-effects-of-the-artificial-surface-of-nanoimprinted-moth-eye-film
#19
Kiyoshi Minoura, Miho Yamada, Takashi Mizoguchi, Toshihiro Kaneko, Kyoko Nishiyama, Mari Ozminskyj, Tetsuo Koshizuka, Ikuo Wada, Tatsuo Suzutani
The antibacterial effect of a nanostructured film, known as "moth-eye film," was investigated. The moth-eye film has artificially formed nano-pillars, consisting of hydrophilic resin with urethane acrylate and polyethylene glycol (PEG) derivatives, all over its surface that replicates a moth's eye. Experiments were performed to compare the moth-eye film with a flat-surfaced film produced from the same materials. The JIS Z2801 film-covering method revealed that the two films produced a decrease in Staphylococcus aureus and Esherichia coli titers of over 5 and 3 logs, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28934124/n-halamine-biocidal-materials-with-superior-antimicrobial-efficacies-for-wound-dressings
#20
Buket Demir, Roy M Broughton, Mingyu Qiao, Tung-Shi Huang, S D Worley
This work demonstrated the successful application of N-halamine technology for wound dressings rendered antimicrobial by facile and inexpensive processes. Four N-halamine compounds, which possess different functional groups and chemistry, were synthesized. The N-halamine compounds, which contained oxidative chlorine, the source of antimicrobial activity, were impregnated into or coated onto standard non-antimicrobial wound dressings. N-halamine-employed wound dressings inactivated about 6 to 7 logs of Staphylococcus aureus and Pseudomonas aeruginosa bacteria in brief periods of contact time...
September 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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