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Staphylococcus aureus

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https://www.readbyqxmd.com/read/28330281/evaluation-of-bioactive-secondary-metabolites-from-endophytic-fungus-pestalotiopsis-neglecta-bab-5510-isolated-from-leaves-of-cupressus-torulosa-d-don
#1
Deeksha Sharma, Avijit Pramanik, Pavan Kumar Agrawal
Six endophytic fungi were isolated from Cupressus torulosa D.Don and identified phenotypically and genotypically. The fungal cultures were further grown and the culture was extracted by two organic solvents methanol and ethyl acetate. The screening was carried out using the agar well diffusion method against human pathogen such as Escherichia coli, Salmonella typhimurium, Bacillus subtilis and Staphylococcus aureus. Isolated strain of Pestalotiopsis sp. was showing prominent antibacterial activity. The crude methanol and ethyl acetate extract of Pestalotiopsis sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330238/isolation-of-antimicrobial-resistant-bacteria-in-upper-respiratory-tract-infections-of-patients
#2
Li-Min Wang, Xiao-Liang Qiao, Liang Ai, Jing-Jing Zhai, Xue-Xia Wang
Haemophilus influenzae, Streptococcus pyogenes, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae are usual cause of upper respiratory tract infection cases. The present study aims the isolation of bacterial strains which are resistant to the commonly prescribed antibiotics. In total, 900 throat swabs were obtained from the patients suffering from upper respiratory tract infections residing in three different localities. The maximum number of isolates (64 %) were obtained from locality-1 (L-1), whereas lowest isolates were found in second locality (L-2)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330192/differential-display-reverse-transcription-pcr-ddrt-pcr-a-new-technology-for-molecular-detection-and-studying-one-of-the-antagonistic-factors-of-bacillus-endophyticus-strain-sa-against-staphylococcus-aureus-mrsa
#3
Mahmoud F Moustafa, Tarek H Taha, M Helal, Sulaiman A Alrumman
Differential Display (DDRT-PCR) is a powerful technique for analyzing differences in gene expression. In-vivo expression technologies and differential display RT-PCR are providing new approaches to further examine a microbe's response to experimental conditions which more closely resemble natural microbial associations and habitats. In this study, Bacillus endophyticus strain SA isolated from the inner tissue of the stem of the cultivated plant (Salvadora persica, Asir, Kingdom of Saudi Arabia) produces an antagonistic factor...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329722/antibacterial-properties-of-amino-acid-functionalized-silver-nanoparticles-decorated-on-graphene-oxide-sheets
#4
Kumudini Chandraker, Rekha Nagwanshi, S K Jadhav, Kallol K Ghosh, Manmohan L Satnami
Graphene oxide (GO) sheets decorated with amino acid L-cysteine (L-cys) functionalized silver nanoparticles (GO-L-cys-Ag) was synthesized by AgNO3, trisodium citrate, and NaBH4. GO-L-cys-Ag nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, ultraviolet-visible (UV-vis) absorption spectra, which demonstrated that a diameter of L-cys-AgNPs compactly deposited on GO. Antibacterial activity tests of GO-L-cys-Ag nanocomposite were carried out using Escherichia coli MTCC 1687 and Staphylococcus aureus MTCC 3160 as model strains of Gram-negative and Gram-positive bacteria, respectively...
March 16, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28329667/serious-infections-are-on-the-rise-in-us-inpatients-with-psoriasis
#5
Derek Y Hsu
Psoriasis is a chronic inflammatory skin disorder associated with immune dysregulation and use of systemic immunosuppressive treatments that may predispose toward serious infections. We sought to determine the rates of serious infections in hospitalized patients with psoriasis. We analyzed data from the 2002-2012 Nationwide Inpatient Sample, containing a 20% sample of all US hospitalizations. Psoriasis was determined by a validated algorithm using ICD-9-CM codes. In multivariate logistic regression models adjusting for socio-demographics, psoriasis was associated with multiple serious infections, including methicillin-resistant Staphylococcus aureus (odds ratio [95% confidence intervals] 1...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329529/concurrent-pyogenic-granuloma-and-bullous-impetigo-of-a-pregnant-woman-s-finger
#6
Rosie Qin, Philip R Cohen
BACKGROUND: Bullous impetigo is a superficial skininfection caused by Staphylococcus aureus (S.aureus). Pyogenic granuloma is a common benigntumor frequently associated with prior trauma.Bullous impetigo and pyogenic granuloma may occurin pregnant women. PURPOSE: The features of a pregnant womanwith pyogenic granuloma and bullous impetigoconcurrently present in a lesion on her finger aredescribed. METHODS: PubMed was used to search the followingterms: bullous impetigo, pregnancy, and pyogenicgranuloma...
March 15, 2017: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329242/vaccination-induces-cd4-t-cell-mediated-mortality-during-staphylococcus-aureus-bacteremia
#7
Hatice Karauzum, Christian C Haudenschild, Ian N Moore, Mahta Mahmoudieh, Daniel L Barber, Sandip K Datta
Multiple candidate vaccines against Staphylococcus aureus infections have failed in clinical trials. Analysis of a recent prematurely halted vaccine trial revealed increased mortality among vaccine recipients who developed post-surgical S. aureus infection, emphasizing the potential for induction of detrimental immune responses and the need to better understand the requirements for protective immunity against S. aureus. These failures of single antigen vaccines have prompted ongoing development of multi-component vaccines to target the multitude of S...
February 21, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28329125/outcomes-in-patients-with-cardiovascular-implantable-electronic-device-infection-managed-with-chronic-antibiotic-suppression
#8
Eugene M Tan, Daniel C DeSimone, M Rizwan Sohail, Larry M Baddour, Walter R Wilson, James M Steckelberg, Abinash Virk
Background: Most cardiovascular implantable electronic device (CIED) recipients are elderly, have multiple comorbid conditions, and are at increased risk of CIED infection (CIEDI). Current guidelines recommend complete device removal in patients with CIEDI to prevent relapse and mortality. However, comorbidities or other factors may preclude device removal, thus prompting a non-surgical approach that includes chronic antibiotic suppression (CAS). There are limited data on outcomes of patients receiving CAS for CIEDI...
February 24, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28328596/antibacterial-effect-of-bacteriocin-isolated-from-lactobacillus-plantarum-atcc-8014-on-postoperative-infection-of-mandibular-fracture-in-vivo
#9
Tao Fu, Yan-Ming Liu
OBJECTIVE: The primary objective of this study was to verify the effects of bacteriocin in treatment of postoperative infection of mandibular fracture in vivo. METHODS: Eighty-two mice were inoculated intravenously with staphylococcal suspensions. Bacterial cultures were obtained from implants. Blood samples were collected at 1, 2, 4, 6, and 8 hours after the injection of bacteriocins. RESULTS: Bacteriocins have significant inhibitory effects on Staphylococcus aureus (P < 0...
March 21, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28328157/lack-of-small-colony-variants-of-staphylococcus-aureus-from-lower-respiratory-tract-specimens
#10
Rosemary Carzino, Emily Hart, Philip Sutton, Louise King, Sarath Ranganathan
BACKGROUND: Small-colony variants (SCVs) of Staphylococcus aureus are associated with worse lung disease in children with Cystic Fibrosis (CF), exhibit a higher resistance to antibiotics and co-colonize more commonly with Pseudomonas aeruginosa compared to the normal phenotype. The prevalence of SCVs in lower airway specimens from children with CF is largely unknown. METHODS: Each visible morphotype of S. aureus was subcultured onto horse blood agar (HBA) to enable identification of SCVs...
March 22, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28328023/identification-of-microorganisms-in-biofluids-of-individuals-with-periodontitis-and-chronic-kidney-disease-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#11
Levy Anderson César Alves, Rafael Celestino Souza, Taciana Mara Couto da Silva, Andreia Watanabe, Meriellen Dias, Maria Anita Mendes, Ana Lídia Ciamponi
RATIONALE: Chronic kidney disease (CKD) and periodontitis (PD) are important health issues. There is a large variety of microorganisms related to the pathogenesis of periodontitis, and optimising the time and the cost of laboratory assays to detect these organisms is highly valuable in the medical field. METHODS: Bacteria were isolated from saliva and oral biofilm of 30 adolescents and young adults with definite medical and dental diagnosis of CKD and PD, respectively, and proteins were extracted for microorganism identification by means of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) technique...
May 30, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28327913/the-use-of-oxford-nanopore-native-barcoding-for-complete-genome-assembly
#12
Sion C Bayliss, Vicky L Hunt, Maho Yokoyama, Harry A Thorpe, Edward J Feil
Background: The Oxford Nanopore Technologies MinION(TM) is a mobile DNA sequencer that can produce long read sequences with a short turn-around time. Here we report the first demonstration of single contig genome assembly using Oxford Nanopore native barcoding when applied to a multiplexed library of 12 samples and combined with existing Illumina short-read data. This paves the way for the closure of multiple bacterial genomes from a single MinION(TM) sequencing run, given the availability of existing short-read data...
February 24, 2017: GigaScience
https://www.readbyqxmd.com/read/28327520/cytotoxicity-bactericidal-and-antioxidant-activity-of-sodium-alginate-hydrosols-treated-with-direct-electric-current
#13
Żaneta Król, Krzysztof Marycz, Dominika Kulig, Monika Marędziak, Andrzej Jarmoluk
The aim of the study was to investigate the effect of using direct electric current (DC) of 0, 200, and 400 mA for five minutes on the physiochemical properties, cytotoxicity, antibacterial, and antioxidant activity of sodium alginate hydrosols with different sodium chloride concentrations. The pH, oxidation-reduction potential (ORP), electrical conductivity (EC), and available chlorine concentration (ACC) were measured. The effect of sodium alginate hydrosols treated with DC on Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Micrococcus luteus, Escherichia coli, Salmonella enteritidis, Yersinia enterocolitica, Pseudomonas fluorescence, and RAW 264...
March 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28327424/cellulase-assisted-extraction-of-polysaccharides-from-malva-sylvestris-process-optimization-and-potential-functionalities
#14
Hosein Rostami, Seyed Mohammad Taghi Gharibzahedi
Enzyme-assisted extraction process of the water-soluble Malva sylvestris polysaccharides (MSPs) was optimized using response surface methodology (RSM). The highest yield (10.40%) of MSPs was achieved at 5.64% cellulase, 55.65°C temperature, 3.4h time, and 5.22 pH. Three homogeneous polysaccharide fractions (MSP-1, MSP-2, MSP-3) were purified by DEAE-cellulose and Sephadex G-100 chromatography, which were composed of galactose, glucuronic acid, arabinose, rhamnose and mannose in different molar ratios with molecular weight range of 2...
March 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28327306/design-synthesis-antibacterial-activity-and-docking-study-of-some-new-trimethoprim-derivatives
#15
Umer Rashid, Waqas Ahmad, Syed Fahad Hassan, Naveeda Akhtar Qureshi, Basit Niaz, Bakhtiar Muhammad, Sameera Imdad, Muhammad Sajid
In present study, nineteen novel trimethoprim (TMP) derivatives were designed, synthesized and evaluated for their antibacterial potential. Hydroxy trimethoprim 2 (HTMP) was synthesized by following the demethylation of 4-methoxy group at trimethoxy benzyl ring of TMP. Structure-activity relationship (SAR) studies were explored on HTMP by incorporating various substituents leading to the identification of some new compounds with improved antibacterial activities. The results revealed that the introduction of benzyloxy (4a-e) and phenyl ethanone (5a-e) group at 4-position of dimethoxy benzyl ring leads to overall increase in the antibacterial activity...
December 1, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28327271/novel-anti-staphylococcal-and-anti-biofilm-properties-of-two-anti-malarial-compounds-mmv665953-1-3-chloro-4-fluorophenyl-3-3-4-dichlorophenyl-urea-and-mmv665807-5-chloro-2-hydroxy-n-3-trifluoromethyl-phenyl-benzamide
#16
Marta Zapotoczna, Nabila Boksmati, Sinead Donohue, Baizurina Bahtiar, Ahmad Boland, Hamzah Al Somali, Alysia Cox, Hilary Humphreys, James P O'Gara, Marian Brennan, Eoghan O'Neill
PURPOSE: The treatment of device-related infections is challenging and current anti-microbial compounds have poor anti-biofilm activity. We aimed to identify and characterize novel compounds effective in the eradication of Staphylococcus aureus biofilms. METHODOLOGY: Two novel compounds, MMV665953 {1-(3-chloro-4-fluorophenyl)-3-(3,4-dichlorophenyl)urea} and MMV665807{5-chloro-2-hydroxy-N-[3-(trifluoromethyl)phenyl]benzamide}, effective in killing S. aureus biofilms, were identified by screening of the open access 'malaria box' chemical library...
March 23, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28326851/evaluation-of-antibacterial-activity-of-the-bark-and-leaf-extracts-of-brosimum-gaudichaudii-tr%C3%A3-cul-against-multidrug-resistant-strains
#17
Joelma da Costa Borges, Michele Cezimbra Perim, Rodrigo Orlandini de Castro, Thiago Antônio de Sousa Araújo, Tadeu José da Silva Peixoto Sobrinho, Ana Carolina Oliveira da Silva, Sandra Maria Botelho Mariano, Solange Cristina Carreiro, Maria Cristina da Silva Pranchevicius
Brosimum gaudichaudii Trécul., a plant that belongs to Moraceae family, is found throughout the Brazilian Cerrado. The antimicrobial activities of ethanolic bark and leaf extracts of B. gaudichaudii were tested against multiresistant bacteria isolated from diabetic foot infections (DFIs). Antimicrobial activity of the extracts was evaluated by agar disc diffusion (DD) and broth dilution (BD) methods. By BD method, bark (53.85, 45.83%) and leaf (42.31, 50.00%) extracts contained antimicrobial activity against both gram-negative and gram-positive bacteria...
March 22, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28326840/a-new-benzophenone-glycoside-from-the-leaves-of-mitracarpus-villosus
#18
Kenneth G Ngwoke, Njideka Orame, Shuai Liu, Festus B C Okoye, Georgios Daletos, Peter Proksch
A new benzophenone glycoside, mitraphenone A (1), together with three known compounds (2-4) were isolated from the leaves of the traditionally used medicinal plant Mitracarpus villosus (Rubiaceae) collected in Nigeria. A combination of one- and two-dimensional NMR spectroscopic and mass spectrometric measurements were carried out to identify the structure of 1. All isolated compounds (1-4) were screened for their antibacterial activity against several Gram-positive and Gram-negative bacteria. Compound 1 exhibited moderate activity against Enterococcus faecium (strains ATCC 35667 and ATCC 700221) and Staphylococcus aureus ATCC 25923 with MIC values ranging from 25 to 50 μM...
March 22, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28326455/neutralization-of-mmp-2-and-tnfr1-regulates-the-severity-of-s-aureus-induced-septic-arthritis-by-differential-alteration-of-local-and-systemic-proinflammatory-cytokines-in-mice
#19
Sahin Sultana, Rana Adhikary, Biswadev Bishayi
Despite advancement in the field of antibiotics septic arthritis remains a serious concern till date. Staphylococcus aureus is the most common bacterium that causes septic arthritis. Severity of this disease is directly correlated with chronic inflammation induced by proinflammatory cytokines like TNF-α, interleukin (IL)-1β, IL-6, and induction of matrix metalloproteinases (MMPs) including MMP-2. The objective of our study was to evaluate the role of MMP-2 and tumor necrosis factor receptor 1 (TNFR1) in the pathogenesis of S...
March 22, 2017: Inflammation
https://www.readbyqxmd.com/read/28325766/staphylococcal-%C3%AE-toxin-modulates-human-aortic-endothelial-cell-and-platelet-function-through-sphingomyelinase-and-biofilm-ligase-activities
#20
Alfa Herrera, Katarina Kulhankova, Vijay K Sonkar, Sanjana Dayal, Aloysius J Klingelhutz, Wilmara Salgado-Pabón, Patrick M Schlievert
Staphylococcus aureus causes many infections, such as skin and soft tissue, pneumonia, osteomyelitis, and infective endocarditis (IE). IE is an endovascular infection of native and prosthetic valves and the lining of the heart; it is characterized by the formation of cauliflower-like "vegetations" composed of fibrin, platelets, other host factors, bacteria, and bacterial products. β-Toxin is an S. aureus virulence factor that contributes to the microorganism's ability to cause IE. This cytolysin has two enzymatic activities: sphingomyelinase (SMase) and biofilm ligase...
March 21, 2017: MBio
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