Islam A Abdelhakim, Yushi Futamura, Yukihiro Asami, Hideaki Hanaki, Naoko Kito, Sachiko Masuda, Arisa Shibata, Atsuya Muranaka, Hiroyuki Koshino, Ken Shirasu, Hiroyuki Osada, Jun Ishikawa, Shunji Takahashi
Actinomycetes are prolific producers of natural products, particularly antibiotics. However, a significant proportion of its biosynthetic gene clusters (BGCs) remain silent under typical laboratory conditions. This limits the effectiveness of conventional isolation methods for the discovery of novel natural products. Genetic interventions targeting the activation of silent gene clusters are necessary to address this challenge. Streptomyces antibiotic regulatory proteins (SARPs) act as cluster-specific activators and can be used to target silent BGCs for the discovery of new antibiotics...
April 23, 2024: Journal of Natural Products