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https://www.readbyqxmd.com/read/28235124/plesiomonas-shigelloides-periprosthetic-knee-infection-after-consumption-of-raw-oysters
#1
Joshua W Hustedt, Sarim Ahmed
Periprosthetic infections are a leading cause of morbidity after total joint arthroplasty. Common pathogens include Staphylococcus aureus, streptococcus, enterococcus, Escherichia coli, and Pseudomonas aeruginosa. However, there are many cases in which rare bacteria are isolated. This case report describes a periprosthetic knee infection caused by Plesiomonas shigelloides. In the United States, P shigelloides and 2 other Vibrionaceae family members, Vibrio vulnificus and Vibrio parahaemolyticus, are most often contracted from eating raw oysters and shellfish...
January 2017: American Journal of Orthopedics
https://www.readbyqxmd.com/read/28235054/synergistic-interactions-between-phenolic-compounds-identified-in-grape-pomace-extract-with-antibiotics-of-different-classes-against-staphylococcus-aureus-and-escherichia-coli
#2
Loreto Sanhueza, Ricardo Melo, Ruth Montero, Kevin Maisey, Leonora Mendoza, Marcela Wilkens
Synergy could be an effective strategy to potentiate and recover antibiotics nowadays useless in clinical treatments against multi-resistant bacteria. In this study, synergic interactions between antibiotics and grape pomace extract that contains high concentration of phenolic compounds were evaluated by the checkerboard method in clinical isolates of Staphylococcus aureus and Escherichia coli. To define which component of the extract is responsible for the synergic effect, phenolic compounds were identified by RP-HPLC and their relative abundance was determined...
2017: PloS One
https://www.readbyqxmd.com/read/28235036/cholesterol-crystals-enhance-tlr2-and-tlr4-mediated-pro-inflammatory-cytokine-responses-of-monocytes-to-the-proatherogenic-oral-bacterium-porphyromonas-gingivalis
#3
Tania Køllgaard, Christian Enevold, Klaus Bendtzen, Peter R Hansen, Michael Givskov, Palle Holmstrup, Claus H Nielsen
Cholesterol deposits and pro-inflammatory cytokines play an essential role in the pathogenesis of atherosclerosis, a predominant cause of cardiovascular disease (CVD). Epidemiological evidence has linked periodontal disease (PD) with atherosclerotic CVD. Accordingly, viable periodontal pathogens, including Porphyromonas gingivalis, have been found in atherosclerotic plaques in humans and mice. We aimed to determine whether cholesterol crystals (CHCs) and oral bacteria synergize in the stimulation of human monocytes...
2017: PloS One
https://www.readbyqxmd.com/read/28233479/the-risk-factors-etiology-and-drug-resistance-of-infection-after-plastic-surgery-and-corresponding-measures
#4
Guangning Wang, Suhong Zhang
BACKGROUND: Explore the risk factors, etiology, and drug resistance of infection after plastic surgery, and present corresponding measures. METHODS: We retrospectively analyzed 980 patients who underwent head and facial plastic surgery from January 2013 to December 2015. Postoperative infection occurred in 169 patients. We analyzed the distribution of pathogenic bacteria in patients undergoing plastic surgery, reviewed the drug resistance of Gram negative (G-) bacteria and Gram positive (G+) bacteria, and analyzed the effects of surgical duration, prophylactic use of antibacterial agents, length of stay (LOS), and preoperative hair removal on infection after plastic surgery...
February 23, 2017: Minerva Chirurgica
https://www.readbyqxmd.com/read/28233476/antimicrobial-activity-of-biogenically-produced-spherical-se-nanomaterials-embedded-in-organic-material-against-pseudomonas-aeruginosa-and%C3%A2-staphylococcus-aureus-strains-on-hydroxyapatite-coated-surfaces
#5
Elena Piacenza, Alessandro Presentato, Emanuele Zonaro, Joseph A Lemire, Marc Demeter, Giovanni Vallini, Raymond J Turner, Silvia Lampis
In an effort to prevent the formation of pathogenic biofilms on hydroxyapatite (HA)-based clinical devices and surfaces, we present a study evaluating the antimicrobial efficacy of Spherical biogenic Se-Nanostructures Embedded in Organic material (Bio Se-NEMO-S) produced by Bacillus mycoides SelTE01 in comparison with two different chemical selenium nanoparticle (SeNP) classes. These nanomaterials have been studied as potential antimicrobials for eradication of established HA-grown biofilms, for preventing biofilm formation on HA-coated surfaces and for inhibition of planktonic cell growth of Pseudomonas aeruginosa NCTC 12934 and Staphylococcus aureus ATCC 25923...
February 23, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28233206/mechanical-ventilator-as-a-major-cause-of-infection-and-drug-resistance-in-intensive-care-unit
#6
Marwa M E Abd-Elmonsef, Dalia Elsharawy, Ayman S Abd-Elsalam
Ventilator-associated pneumonia (VAP) is the most frequent infection in intensive care units (ICU). It is associated with high rates of long morbidity and mortality. Management of a case of VAP is often said to add $40,000 to hospital costs USA. All these data directed our interest to study the etiology, risk factors, and antibiotic susceptibility patterns of VAP in ICU of Tanta University Hospital. This study included 36 cases of VAP. Endotracheal aspirates were obtained from all cases and microbiologically analyzed...
February 23, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28232829/dexamethasone-inhibits-s-aureus-induced-neutrophil-extracellular-pathogen-killing-mechanism-possibly-through-toll-like-receptor-regulation
#7
Ting Wan, Yingying Zhao, Fangli Fan, Renjian Hu, Xiuming Jin
Neutrophils release neutrophil extracellular traps (NETs) in a pathogen-killing process called NETosis. Excessive NETs formation, however, is implicated in disease pathogenesis. Therefore, to understand how NETosis is regulated, we examined the effect of dexamethasone (DXM), an anti-inflammatory drug, on this process and the role of toll-like receptors (TLRs). We stimulated human neutrophils with phorbol 12-myristate 13-acetate (PMA) or Staphylococcus aureus (S. aureus) and quantified NETs formation. We also examined the effect of DXM on the bactericidal effect of NETs and the role of reactive oxygen species (ROS) and nuclear factor (NF)-κB in DXM-regulated NETosis...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28232575/the-cytotoxic-staphylococcus-aureus-psm%C3%AE-3-reveals-a-cross-%C3%AE-amyloid-like-fibril
#8
Einav Tayeb-Fligelman, Orly Tabachnikov, Asher Moshe, Orit Goldshmidt-Tran, Michael R Sawaya, Nicolas Coquelle, Jacques-Philippe Colletier, Meytal Landau
Amyloids are ordered protein aggregates, found in all kingdoms of life, and are involved in aggregation diseases as well as in physiological activities. In microbes, functional amyloids are often key virulence determinants, yet the structural basis for their activity remains elusive. We determined the fibril structure and function of the highly toxic, 22-residue phenol-soluble modulin α3 (PSMα3) peptide secreted by Staphylococcus aureus PSMα3 formed elongated fibrils that shared the morphological and tinctorial characteristics of canonical cross-β eukaryotic amyloids...
February 24, 2017: Science
https://www.readbyqxmd.com/read/28232428/complete-genome-and-plasmid-sequences-of-staphylococcus-aureus-edcc-5055-dsm-28763-used-to-study-implant-associated-infections
#9
Gopala Krishna Mannala, Torsten Hain, Cathrin Spröer, Boyke Bunk, Jörg Overmann, Volker Alt, Eugen Domann
Staphylococcus aureus EDCC 5055 (DSM 28763) is a human clinical wound isolate intensively used to study implant-associated infections in rabbit and rat infection models. Here, we report its complete genome sequence (2,794,437 bp) along with that of one plasmid (27,437 bp). This strain belongs to sequence type 8 and contains a mecA gene.
February 23, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28232311/reply-to-modulatory-effects-of-a-subinhibitory-concentration-of-clindamycin-in-community-acquired-methicillin-resistant-staphylococcus-aureus-strains-of-sequence-type-30
#10
Katrin Schilcher, Vanina Dengler Haunreiter, Annelies S Zinkernagel
No abstract text is available yet for this article.
March 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28232309/ceftaroline-resistant-daptomycin-tolerant-and-heterogeneous-vancomycin-intermediate-methicillin-resistant-staphylococcus-aureus-causing-infective-endocarditis
#11
Masayuki Nigo, Lorena Diaz, Lina P Carvajal, Truc T Tran, Rafael Rios, Diana Panesso, Juan D Garavito, William R Miller, Audrey Wanger, George Weinstock, Jose M Munita, Cesar A Arias, Henry F Chambers
We report a case of infective endocarditis (IE) caused by ceftaroline-resistant, daptomycin-tolerant, and heterogeneous vancomycin-intermediate methicillin-resistant S. aureus (MRSA). Resistance to ceftaroline emerged in the absence of drug exposure, and the E447K substitution in the active site of PBP2a previously associated with ceftaroline resistance was identified. Additionally, we present evidence of patient-to-patient transmission of the strain within the same unit. This case illustrates the difficulties in treating MRSA IE in the setting of a multidrug-resistant phenotype...
March 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28232132/a-novel-anti-lipopolysaccharide-factor-spalf6-in-mud-crab-scylla-paramamosain-exhibiting-different-antimicrobial-activity-from-its-single-amino-acid-mutant
#12
Zhi-Guo Hou, Yuan Wang, Kaimin Hui, Wen-Hong Fang, Shu Zhao, Jing-Xiao Zhang, Hongyu Ma, Xin-Cang Li
In crustaceans, anti-lipopolysaccharide factors (ALFs) are important immune effectors that have sequence diversity and exhibit broad antimicrobial activities. In this study, we characterized a novel ALF homolog SpALF6 from mud crab Scylla paramamosain and its variant SpALF6-V, which was generated by mutations of two amino acids (H46 to R and A110 to P) due to the presence of two single nucleotide polymorphisms (SNPs). SpALF6 was an anionic peptide with isoelectric point (pI) 6.79, whereas SpALF6-V was a cationic protein with pI 7...
February 20, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28231891/-infections-in-cystic-fibrosis-up-to-date
#13
C Marguet, L Lémée, H Morisse-Pradier, L Couderc
This review focused on the news in CF airways infection. International guidelines were provided for the care of non tuberculous mycobacteria, and recent studies stressed on the benefit effect of azithromycin or combined antibiotics. The identification of multiresistant environmental bacteria in airways made to account for little-known consequences. Early diagnosis and eradication of Pseudomonas aeruginosa and Staphylococcus aureus methi-R were still a concern, and reports were proposed. However, the studies on staphylococcus methi-R should be interpreted as regards the European or American continent...
December 2016: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/28231850/decline-in-the-proportion-of-methicillin-resistance-among-staphylococcus-aureus-isolates-from-non-invasive-samples-and-in-outpatient-settings-and-changes-in-the-co-resistance-profiles-an-analysis-of-data-collected-within-the-antimicrobial-resistance-surveillance
#14
Jan Walter, Ines Noll, Marcel Feig, Bettina Weiss, Hermann Claus, Guido Werner, Tim Eckmanns, Julia Hermes, Muna Abu Sin
BACKGROUND: Recent analysis of trends of non-invasive infections with methicillin resistant Staphylococcus aureus (MRSA), of trends of MRSA infections in outpatient settings and of co-resistance profiles of MRSA isolates are scarce or lacking in Germany. METHODS: We analysed data from the Antimicrobial Resistance Surveillance Network (ARS). We included in the analysis the first isolate of S. aureus per patient and year, which had a valid test result for oxacillin resistance and which was not a screening sample...
February 23, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28231648/evaluation-of-infection-after-anterior-cruciate-ligament-reconstruction-during-a-short-period
#15
Hee-June Kim, Hyun-Joo Lee, Jong-Chul Lee, Seung-Gi Min, Hee-Soo Kyung
Purpose: We encountered 7 cases (7.1%) of infection (5 deep and 2 superficial) in 98 cases of anterior cruciate ligament (ACL) reconstruction using hamstring autografts in a 17-month period. The aim of this study was to analyze the causes of infection and to introduce our treatment strategy. Materials and Methods: We investigated the shelf-life of the fixation implants, the order of surgery, previous knee surgery, infectious pathogen, treatment of infection, and results of treatment...
March 1, 2017: Knee Surgery & related Research
https://www.readbyqxmd.com/read/28231455/synthetic-investigation-physicochemical-characterization-and-antibacterial-evaluation-of-ternary-bi-iii-systems-with-hydroxycarboxylic-acid-and-aromatic-chelator-substrates
#16
C M Nday, E Halevas, A Tsiaprazi-Stamou, D Eleftheriadou, A Hatzidimitriou, G Jackson, D Reid, A Salifoglou
Due to its physical and chemical properties, bismuth (Bi(III)) is widely used in the treatment of several gastrointestinal and skin diseases, and infections caused by bacteria. Herein, its known antimicrobial potential was taken into consideration in the synthesis of two new hybrid ternary materials of Bi(III) with the physiological α-hydroxycarboxylic glycolic acid and 1,10-phenanthroline (phen), [Bi2(C2H2O3)2(C2H3O3)(NO3)]n(.) nH2O (1) and [Bi(C12H8N2)(NO3)4](C10H8N4) (2), aiming at improving its antibacterial properties...
February 11, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28231167/antibacterial-activity-of-fructus-forsythia-essential-oil-and-the-application-of-eo-loaded-nanoparticles-to-food-borne-pathogens
#17
Na Guo, Qing-Yan Gai, Jiao Jiao, Wei Wang, Yuan-Gang Zu, Yu-Jie Fu
Fructus forsythia essential oil (FEO) with excellent antibacterial activity was rarely reported. The objective of the present study was to investigate the antibacterial activity and the antibacterial mechanism of FEO against two food-borne pathogenic bacteria, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in vitro. When treated FEO, the zones of inhibition (ZOI) of E. coli (20.5 ± 0.25 mm) and S. aureus (24.3 ± 0.21 mm) were much larger than control (p < 0.05). The minimum inhibitory concentrations (MICs) of FEO were 3...
October 29, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28231036/aqueous-humor-penetration-and-biological-activity-of-moxifloxacin-0-5-ophthalmic-solution-alone-or-with-dexamethasone-0-1
#18
Rachel L R Gomes, Rodrigo Galvão Viana, Luiz Alberto S Melo, Alessandro Carvalho Cruz, Eunice Mayumi Suenaga, Kenneth R Kenyon, Mauro Campos
PURPOSE: To compare aqueous humor concentrations of topically applied moxifloxacin 0.5% ophthalmic solution alone or in combination with dexamethasone 0.1% and to correlate these concentrations with the minimum inhibitory concentrations (MICs) for common endophthalmitis-causing organisms. METHODS: Sixty-eight patients undergoing routine phacoemulsification with intraocular lens implantation received either moxifloxacin 0.5% alone or moxifloxacin 0.5% combined with dexamethasone...
March 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28230780/a3r-phage-and-staphylococcus-aureus-lysate-do-not-induce-neutrophil-degranulation
#19
Jan Borysowski, Ryszard Międzybrodzki, Piotr Wierzbicki, Danuta Kłosowska, Grażyna Korczak-Kowalska, Beata Weber-Dąbrowska, Andrzej Górski
The objective of this study was to evaluate the effects of A3R phage and Staphylococcus aureus lysate obtained after phage infection on neutrophil degranulation. The exocytosis of primary and secondary granules from neutrophils was investigated in vitro in whole blood specimens by flow cytometry based on the expression of specific markers of exocytosis (CD63 for primary granules and CD66b for secondary granules). We found that both A3R and S. aureus lysate had no significant effect on the exocytosis of primary and secondary granules...
February 21, 2017: Viruses
https://www.readbyqxmd.com/read/28229435/design-and-pharmacodynamics-of-recombinant-nz2114-histidine-mutants-with-improved-activity-against-methicillin-resistant-staphylococcus-aureus
#20
Huixian Chen, Ruoyu Mao, Da Teng, Xiumin Wang, Ya Hao, Xingjun Feng, Jianhua Wang
NZ2114 is a promising candidate for therapeutic application owing to its potent activity to Staphylococcus aureus. Our objective was to identify NZ2114 derivatives with improved activity through substitution of His16 and His18 with residues Arginine and Lysine. Eight mutants were designed and expressed in Pichia pastoris X-33 via pPICZαA. Five of them exhibited strong antimicrobial activity against S. aureus at low minimal inhibitory concentrations (MICs) of 0.057-0.454 μM. Among them, H1, H2, and H3 showed ideal pharmacodynamic effects on methicillin-resistant S...
December 2017: AMB Express
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