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https://www.readbyqxmd.com/read/28822261/revelation-of-susceptibility-differences-due-to-hg-ii-accumulation-in-streptococcus-pyogenes-against-cx-agnps-and-cefixime-by-atomic-force-microscopy
#1
Wasia Rasheed, Muhammad Raza Shah, Samina Perveen, Shakil Ahmed, Sami Uzzaman
Solution based method for the formation of chemically modified silver nanoparticles (CX-AgNPs) using Cefixime as stabilizing and reducing agent was developed. The CX-AgNPs were characterized by AFM, UV-visible, FT-IR and MALDI-TOF MS. Bactericidal efficiency of CX-AgNPs and Cefixime against Streptococcus pyogenes was evaluated. Afterwards, susceptibility differences of Streptococcus pyogenes due to accumulation of Hg(II) against CX-AgNPs and Cefixime were estimated and validated through Atomic force microscopy...
August 16, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28822224/is-it-necessary-for-all-samples-to-quantify-25ohd2-and-25ohd3-using-lc-ms-ms-in-clinical-practice
#2
Songlin Yu, Ruiping Zhang, Weiyan Zhou, Xinqi Cheng, Qian Cheng, Liangyu Xia, Shaowei Xie, Yicong Yin, Dandan Sun, Wei Su, Ling Qiu
BACKGROUND: The demand for vitamin D testing is increasing in China. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) immunoassay is specific and accurate but requires expensive equipment, experienced operators, and complicated pretreatment of serum. Automated immunoassays are simple and convenient but only determine total 25-hydroxyvitamin D (25OHD). The objective of this study was to quantify 25OHD2 and 25OHD3 in patients to assist clinical physicians and laboratory directors in choosing the most appropriate method to determine 25OHD...
August 19, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28822196/-metabolic-fingerprint-analysis-of-raw264-7-inflammatory-cell-model-by-using-uplc-q-tof-ms
#3
Shan-Shan Gao, Hui-Qing Guo, Ze-Kun Zhang, Guang-Can Bai, Xiao-Yan Gao, Chang-Hua Ma
In order to reveal the properties of polar metabolome in inflammatory cells, we selected LPS-induced RAW264.7 inflammatory cell models as the carrier for the research of metabolic fingerprint analysis. In this study, an ultra performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomics protocol was optimized for the extraction of polar metabolites from RAW264.7 cell line. Then orthogonal partial least squares discriminant analysis (OPLS-DA) was used to process the metabolic data, and finally, a total of 17 metabolites were selected and identified...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822187/-enzymatic-glycosylation-of-4-demethylepipodophyllotoxin
#4
Ke-Bo Xie, Ri-Dao Chen, Yu-Jiao Zhang, Da-Wei Chen, Jun-Gui Dai
The glycosides of 4'-demethylepipodophyllotoxin (DMEP) possess various pharmacological activities; however, the chemical synthesis of these glycosides faces challenges in regioselectivity, stereoselectivity, and the protection and de-protection of functional groups. In this work, a novel glycosyltransferase (GT) gene AbGT5 from Aloe barbadensis was successfully cloned, heterogeneously expressed and purified. Recombinant AbGT5 was able to catalyze the glycosylation of DMEP and the glycosylated product, which was separated from the preparative scale reaction, was characterized as DMEP 4'-O-β-D-glucoside via MS, 1H-NMR, 13C-NMR, HSQC and HMBC...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822186/-lignan-glycosides-and-sucrose-esters-from-roots-of-securidaca-inappendiculata
#5
Zhi Wang, Xue-Dong Yang, Hai-Yan Zha, Li-Na Guo, Dan Shen, Li-Zhen Xu, Shi-Lin Yang
Nine compounds, including five lignan glycosides (1-5), three sucrose esters (6-8), and one organic acid ester (9), were isolated from the ethanol extract of the roots of Securidaca inappendiculata by various chromatographic methods including silica gel, MPLC and preparative HPLC. Their structures were elucidated as acernikol-4″-O-β-D-glucopyranoside (1), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside (2), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside (3), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9'-O-β-D-glucopyranoside (4), (7R, 8S)-5-methoxydihydrodehy-drodiconiferyl alcohol 4-O-β-D-glucopyranoside (5), 3, 6'-O-diferuloylsucrose (6), 3-O-feruloyl-6'-O-sinapoylsucrose (7), sibricose A5 (8), and mehyl ferulate (9) on the basis of 1H-, 13C-NMR and MS experiments...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822164/-analysis-on-microdialysis-probe-recovery-of-baicalin-in-vitro-and-in-vivo-based-on-lc-ms-ms
#6
Teng-Fei Chen, Jian-Xun Liu, Ying Zhang, Li Lin, Wen-Ting Song, Ming-Jiang Yao
To further study the brain behavior and the pharmacokinetics of baicalin in intercellular fluid of brain, and study the recovery rate and stability of brain and blood microdialysis probe of baicalin in vitro and in vivo. The concentration of baicalin in brain and blood microdialysates was determined by LC-MS/MS and the probe recovery for baicalin was calculated. The effects of different flow rates (0.50, 1.0, 1.5, 2.0,3.0 μL•min⁻¹) on recovery in vitro were determined by incremental method and decrement method...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822163/-mechanism-of-treatment-effect-of-huanglian-huangqin-herb-pairs-on-cerebral-ischemia-rats-based-on-metabolomic-approach
#7
Hui-Ting Cao, Hua-Xu Zhu, Qi-Chun Zhang, Li-Wei Guo
The metabolic effect of Huanglian-Huangqin herb pairs on cerebral ischemia rats was studied by using metabolomic method. The rat model of ischemia reperfusion injury induced by introduction of transient middle cerebral artery occlusion (MCAO) followed by reperfusion. Ultra high performance liquid chromatography-series four pole time of flight mass spectrometry method(UPLC-Q-TOF/MS), Markerlynx software, and principal component analysis and partial least-squares discriminant analysis were used to analyze the different endogenous metabolites among the urine samples of sham rats, cerebral ischemia model rats, Huanglian groups (HL), Huangqin groups (HQ) and Huanglian-Huangqin herb pairs groups (LQ) was achieved, combined with accurate information about the endogenous metabolites level and secondary fragment ions, retrieval and identification of possible biological markers, metabolic pathway which build in MetPA database...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822158/-fast-identification-of-constituents-of-lagotis-brevituba-by-using-uplc-q-tof-ms-ms-method
#8
Jing Xie, Li Zhang, Jin-Xiang Zeng, Min Li, Juan Wang, Xiong-Xiong Xie, Guo-Yue Zhong, Guang-Ming Luo, Jin-Bin Yuan, Jian Liang
The chemical constituents of Lagotis brevituba were rapidly determined and analyzed by using ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) method, providing material basis for the clinical application of L. brevituba. The separation was performed on UPLC YMC-Triart C₁₈ (2.1 mm×100 mm, 1.9 μm) column, with acetonitrile-water containing 0.2% formic acid as mobile phase for gradient elution. The flow rate was 0.4 mL•min-1 gradient elution and column temperature was 40 ℃, the injection volume was 2 μL...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822156/-hplc-ms-ms-determination-of-residual-amount-of-4-plant-growth-retardants-in-6-dried-root-and-rhizome-herbs
#9
Yu-Yao Zhai, Bao-Lin Guo
HPLC-MS/MS was applied to the determination of residual amount of plant growth retardant such as paclobutrazol, daminozide, chlormequat and mepiquat chloride in dried root and rhizome herbs. The sample was extracted twice with acetonitrile containing 0.1% formic acid. The separation was performed on a Waters Atlantis HILIC column with an elution system consisting of acetonitrile-5 mmol•L⁻¹ ammonium acetate solution with 0.1% formic acid, methanol and acetonitrile. The MS spectrum was acquired in positive mode with multiple reactions monitoring (MRM)...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822133/isotopically-nonstationary-metabolic-flux-analysis-inst-mfa-of-photosynthesis-and-photorespiration-in-plants
#10
Fangfang Ma, Lara J Jazmin, Jamey D Young, Doug K Allen
Photorespiration is a central component of photosynthesis; however to better understand its role it should be viewed in the context of an integrated metabolic network rather than a series of individual reactions that operate independently. Isotopically nonstationary (13)C metabolic flux analysis (INST-MFA), which is based on transient labeling studies at metabolic steady state, offers a comprehensive platform to quantify plant central metabolism. In this chapter, we describe the application of INST-MFA to investigate metabolism in leaves...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822128/quantification-of-photorespiratory-intermediates-by-mass-spectrometry-based-approaches
#11
Stéphanie Arrivault, Toshihiro Obata
Photorespiration is an essential metabolic process in plants occurring via the oxygenase reaction of RuBisCO. In order to understand this process, it is essential to determine the amounts of intermediates involved. For this purpose we combined mass spectrometry-based approaches and the use of authentic standards for the quantification of photorespiratory intermediates. Here we describe protocols for the extraction and quantification of 2-phosphoglycolate (2PG) by LC-MS/MS and serine, glycine, glycolate, hydroxypyruvate, glyoxylate, and glycerate by GC-MS...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822126/dimethyl-labeling-based-quantification-of-the-lysine-acetylome-and-proteome-of-plants
#12
Ines Lassowskat, Markus Hartl, Fabian Hosp, Paul J Boersema, Matthias Mann, Iris Finkemeier
Photorespiratory enzymes in different cellular compartments have been reported to be posttranslational modified by phosphorylation, disulfide formation, S-nitrosylation, glutathionylation, and lysine acetylation. However, not much is known yet about the function of these modifications to regulate the activities, localizations, or interactions of the proteins in this metabolic pathway. Hence, it will be of great importance to study these modifications and their temporal and conditional occurrence in more detail...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822119/five-questions-answered-a-review-of-autologous-hematopoietic-stem-cell-transplantation-for-the-treatment-of-multiple-sclerosis
#13
REVIEW
Harold L Atkins, Mark S Freedman
Multiple sclerosis (MS) is thought to be an autoimmune disease targeting the central nervous system leading to demyelination, and axonal and neuronal damage, resulting in progressive disability. More intensive therapies such as immunodepletion with hematopoietic stem-cell rescue are being used at a time prior to patients becoming irreversibly disabled. Over the last 15 years, there has been a shift away from using autologous hematopoietic stem-cell transplants (aHSCT) to treat patients with progressive MS, towards treating those with active inflammation and relapses...
August 18, 2017: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
https://www.readbyqxmd.com/read/28822061/co-ultramicronized-palmitoylethanolamide-luteolin-facilitates-the-development-of-differentiating-and-undifferentiated-rat-oligodendrocyte-progenitor-cells
#14
Stephen D Skaper, Massimo Barbierato, Laura Facci, Mila Borri, Gabriella Contarini, Morena Zusso, Pietro Giusti
Oligodendrocytes, the myelin-producing cells of the central nervous system (CNS), have limited capability to bring about repair in chronic CNS neuroinflammatory demyelinating disorders such as multiple sclerosis (MS). MS lesions are characterized by a compromised pool of undifferentiated oligodendrocyte progenitor cells (OPCs) unable to mature into myelin-producing oligodendrocytes. An attractive strategy may be to replace lost OLs and/or promote their maturation. N-palmitoylethanolamine (PEA) is an endogenous fatty acid amide signaling molecule with anti-inflammatory and neuroprotective actions...
August 18, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28822035/identification-and-functional-characterization-of-a-p-coumaroyl-coa-2-hydroxylase-involved-in-the-biosynthesis-of-coumarin-skeleton-from-peucedanum-praeruptorum-dunn
#15
Ruolan Yao, Yucheng Zhao, Tingting Liu, Chuanlong Huang, Sheng Xu, Ziwei Sui, Jun Luo, Lingyi Kong
A p-coumaroyl CoA 2'-hydroxylase responsible for the formation of coumarin lactone ring was identified from Peucedanum praeruptorum Dunn and functionally characterized in vitro. Coumarins are important plant secondary metabolites with a variety of biological activities. Ortho-hydroxylation of cinnamates leads to the formation of coumarin lactone ring and is generally thought to be a key step in coumarin biosynthesis. However, ortho-hydroxylases, especially p-coumaroyl CoA 2'-hydroxylase (C2'H) responsible for the biosynthesis of the most common coumarin skeleton, have received insufficient attention...
August 18, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28821947/the-influence-of-microvascular-injury-on-native-t1-and-t2-relaxation-values-after-acute-myocardial-infarction-implications-for-non-contrast-enhanced-infarct-assessment
#16
Lourens F H J Robbers, Robin Nijveldt, Aernout M Beek, Paul F A Teunissen, Maurits R Hollander, P Stefan Biesbroek, Henk Everaars, Peter M van de Ven, Mark B M Hofman, Niels van Royen, Albert C van Rossum
OBJECTIVES: Native T1 mapping and late gadolinium enhancement (LGE) imaging offer detailed characterisation of the myocardium after acute myocardial infarction (AMI). We evaluated the effects of microvascular injury (MVI) and intramyocardial haemorrhage on local T1 and T2* values in patients with a reperfused AMI. METHODS: Forty-three patients after reperfused AMI underwent cardiovascular magnetic resonance imaging (CMR) at 4 [3-5] days, including native MOLLI T1 and T2* mapping, STIR, cine imaging and LGE...
August 18, 2017: European Radiology
https://www.readbyqxmd.com/read/28821874/global-methylation-correlates-with-clinical-status-in-multiple-sclerosis-patients-in-the-first-year-of-ifnbeta-treatment
#17
María Jesús Pinto-Medel, Begoña Oliver-Martos, Patricia Urbaneja-Romero, Isaac Hurtado-Guerrero, Jesús Ortega-Pinazo, Pedro Serrano-Castro, Óscar Fernández, Laura Leyva
The alteration of DNA methylation patterns are a key component of disease onset and/or progression. Our objective was to evaluate the differences in Long Interspersed Nuclear Element-1 (LINE-1) methylation levels, as a surrogate marker of global DNA methylation, between multiple sclerosis (MS) patients and healthy controls. In addition, we assessed the association of LINE-1 methylation with clinical disease activity in patients treated with IFNbeta (IFNβ). We found that individuals with high levels of LINE-1 methylation showed 6-fold increased risk of suffering MS...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821789/isolation-characterization-and-toxicological-potential-of-alternaria-mycotoxins-tea-aoh-and-ame-in-different-alternaria-species-from-various-regions-of-india
#18
Mukesh Meena, Prashant Swapnil, R S Upadhyay
Alternaria species produce various sorts of toxic metabolites during their active growth and causes severe diseases in many plants by limiting their productivity. These toxic metabolites incorporate various mycotoxins comprising of dibenzo-α-pyrone and some tetramic acid derivatives. In this study, we have screened out total 48 isolates of Alternaria from different plants belonging to different locations in India, on the basis of their pathogenic nature. Pathogenicity testing of these 48 strains on susceptible tomato variety (CO-3) showed 27...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821776/mineralization-and-preservation-of-an-extremotolerant-bacterium-isolated-from-an-early-mars-analog-environment
#19
F Gaboyer, C Le Milbeau, M Bohmeier, P Schwendner, P Vannier, K Beblo-Vranesevic, E Rabbow, F Foucher, P Gautret, R Guégan, A Richard, A Sauldubois, P Richmann, A K Perras, C Moissl-Eichinger, C S Cockell, P Rettberg, Marteinsson, E Monaghan, P Ehrenfreund, L Garcia-Descalzo, F Gomez, M Malki, R Amils, P Cabezas, N Walter, F Westall
The artificial mineralization of a polyresistant bacterial strain isolated from an acidic, oligotrophic lake was carried out to better understand microbial (i) early mineralization and (ii) potential for further fossilisation. Mineralization was conducted in mineral matrixes commonly found on Mars and Early-Earth, silica and gypsum, for 6 months. Samples were analyzed using microbiological (survival rates), morphological (electron microscopy), biochemical (GC-MS, Microarray immunoassay, Rock-Eval) and spectroscopic (EDX, FTIR, RAMAN spectroscopy) methods...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821774/pre-coincidence-brain-activity-predicts-the-perceptual-outcome-of-streaming-bouncing-motion-display
#20
Song Zhao, Yajie Wang, Lina Jia, Chengzhi Feng, Yu Liao, Wenfeng Feng
When two identical visual discs move toward each other on a two-dimensional visual display, they can be perceived as either "streaming through" or "bouncing off" each other after their coincidence. Previous studies have observed a strong bias toward the streaming percept. Additionally, the incidence of the bouncing percept in this ambiguous display could be increased by various factors, such as a brief sound at the moment of coincidence and a momentary pause of the two discs. The streaming/bouncing bistable motion phenomenon has been studied intensively since its discovery...
August 18, 2017: Scientific Reports
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