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María Jesús García-Sarrió, María Luz Sanz, Jesús Sanz, Azucena González-Coloma, Ana Cristina Soria
A new microwave assisted extraction (MAE) method using ethanol as solvent has been optimized by means of a Box-Behnken experimental design for the enhanced extraction of bioactive terpenoids from Mentha rotundifolia leaves; 100°C, 5 min, 1.125 g dry sample:10 mL solvent and a single extraction cycle were selected as optimal conditions. Improved performance of MAE method in terms of extraction yield and/or reproducibility over conventional solid-liquid extraction and ultrasound assisted extraction was also previously assessed...
April 14, 2018: Electrophoresis
Fatemeh Shirbani, Edward Barin, Yi-Ching Lee, Karl Ng, John Douglas Edward Parratt, Mark Butlin, Alberto P Avolio
BACKGROUND: Prevalence of cardiovascular autonomic dysfunction (CAD) in multiple sclerosis (MS) varies between studies. Cardiac autonomic function is usually assessed by cardiovascular reflex tests. We hypothesized that MS is associated with CAD, quantifiable by non-invasive means including quantification of baroreceptor sensitivity (BRS) and heart rate variability. METHODS: In this study a comprehensive suite of cardiovascular autonomic tests based only on the spontaneous changes of heart rate and blood pressure was applied to 23 MS patients and age and gender-matched controls...
March 27, 2018: Multiple Sclerosis and related Disorders
María L Martínez-Ginés, Jose M García-Domínguez, Lucía Forero, Neus Canal, Pablo Rebollo, Daniel Prefasi, Cynthia A Honan, Jorge Maurino
INTRODUCTION: Unemployment is a significant problem for people with multiple sclerosis (MS). The MS Work Difficulties Questionnaire (MSWDQ-23) is a self-report tool to assess work-related problems in people with MS across three domains: physical, psychological/cognitive, and external barriers. The aim of this study was to assess the psychometric properties of the Spanish version of the MSWDQ-23. METHODS: A multicentre, non-interventional, cross-sectional study in adult patients with relapsing-remitting multiple sclerosis (RRMS) or primary progressive (PPMS) multiple sclerosis (McDonald 2010 criteria) was conducted...
April 3, 2018: Multiple Sclerosis and related Disorders
Jaya Sharma, Kashif Shamim, Santosh Kumar Dubey
Achromobacter xylosoxidans strain SJ11, tolerating up to 4.0 mM lead nitrate, in a defined minimal medium was isolated from the waste of a battery manufacturing industry, Goa, India. Interestingly, it formed white precipitate on exposure to lead nitrate which was also evident from scanning electron micrograph (SEM). Energy dispersive X-ray spectroscopic analysis revealed the presence of lead (48.5% by weight) along with phosphorus and chlorine in the precipitate. Transmission electron microscopy (TEM) of bacterial cells clearly refuted the possibility of intracellular lead uptake confirming extracellular precipitation as a predominant mechanism of lead resistance in this bacterium...
April 12, 2018: Journal of Environmental Management
Dattatray K Bedade, Rekha S Singhal
Acrylamide is neurotoxic, genotoxic, teratogenic and carcinogenic. Its widespread use in various industrial processes leads to environmental contamination. Acrylamidase produced by certain bacteria degrade acrylamide to acrylic acid and ammonia. The present study details the isolation and identification of soil bacterium which could degrade acrylamide. Among the 18 acrylamide-degrading isolates tested, isolate ICTDB921 demonstrated superior acrylamide degradation which was confirmed by HPLC, FTIR and GC-MS...
April 6, 2018: Bioresource Technology
Zhen Long, Jia Li, Zhimou Guo, Zhaoqi Zhan, Yueqi Li, Yutian Wang, Changkun Li, Feng Ji, Lixiao Li, Taohong Huang
The conventional UV/Vis spectroscopy methods recommended by the European Pharmacopoeia (EP) for determining hexosamine, hexonic acid and methylpentose in pneumococcal polysaccharide vaccine (PPSV) hydrolates are time-consuming due to derivatization process (typically, an analysis cycle is more than 4 h) and improvements of selectivity and precision of the methods are in demand. In this study, a new approach based on hydrophilic interaction liquid chromatography and triple quadrupole mass spectrometry (HILIC-MS/MS) was optimized to overcome the drawbacks of the EP methods for simultaneous determination of methylpentose, hexose, hexosamine and hexonic acid in PPSV hydrolysates...
April 7, 2018: Journal of Pharmaceutical and Biomedical Analysis
Feliks Kogan, Audrey P Fan, Uche Monu, Andrei Iagaru, Brian A Hargreaves, Garry E Gold
OBJECTIVE: To investigate changes in bone metabolism by PET, as well as spatial relationships between bone metabolism and MRI quantitative markers of early cartilage degradation, in ACL-reconstructed knees. DESIGN: Both knees of 15 participants with unilateral reconstructed ACL tears and unaffected contralateral knees were scanned using a simultaneous 3.0T PET-MRI system following injection of 18 F-sodium fluoride(18 F-NaF). The maximum pixel standardized uptake value(SUVmax ) in the subchondral bone and the average T2 relaxation time in cartilage were measured in each knee in 8 knee compartments...
April 12, 2018: Osteoarthritis and Cartilage
Takatoshi Yonezawa, Tomohisa Watari, Kazuho Ashizawa, Daisuke Hanada, Takako Yanagiya, Naoki Watanabe, Takashi Terada, Yutaka Tomoda, Satoshi Fujii
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been incorporated into pathogenic bacterial identification methods and has improved their rapidity. Various methods have been reported to directly identify bacteria with MALDI-TOF MS by pretreating culture medium in blood culture bottles. Rapid BACpro® (Nittobo Medical Co., Ltd.) is a pretreatment kit for effective collection of bacteria with cationic copolymers. However, the Rapid BACpro® pretreatment kit is adapted only for MALDI Biotyper (Bruker Daltonics K...
April 12, 2018: Journal of Microbiological Methods
Antoni Olona, Ximena Terra, Jeong-Hun Ko, Carme Grau-Bové, Montserrat Pinent, Anna Ardevol, Ana Garcia Diaz, Aida Moreno-Moral, Matthew Edin, David Bishop-Bailey, Darryl C Zeldin, Timothy J Aitman, Enrico Petretto, Mayte Blay, Jacques Behmoaras
OBJECTIVE: When molecular drivers of healthy adipogenesis are perturbed, this can cause hepatic steatosis. The role of arachidonic acid (AA) and its downstream enzymatic cascades, such as cyclooxygenase, in adipogenesis is well established. The exact contribution of the P450 epoxygenase pathway, however, remains to be established. Enzymes belonging to this pathway are mainly encoded by the CYP2J locus which shows extensive allelic expansion in mice. Here we aimed to establish the role of endogenous epoxygenase during adipogenesis under homeostatic and metabolic stress conditions...
March 9, 2018: Molecular Metabolism
Ioanna Dagiasi, Victor Vall, Eva Kumlien, Joachim Burman, Johan Zelano
PURPOSE: The prevalence of epilepsy is increased in multiple sclerosis (MS), but information on AED treatment and seizure outcome is scarce. We describe epilepsy characteristics including the use of AEDs and proportion of seizure-free patients at two tertiary hospitals in Sweden. METHOD: We retrospectively studied electronic medical records of all patients with a diagnosis of MS and seizures at Sahlgrenska university hospital and Uppsala university hospital. Clinical data were reviewed until 2017...
April 5, 2018: Seizure: the Journal of the British Epilepsy Association
O Morgan Hall, Cody J Peer, Courtney D Fitzhugh, William D Figg
Analysis of cyclophosphamide (CP) and its metabolite, 4-hydroxycyclophosphamide (4OHCP), in a single assay has the ability to improve sampling techniques benefitting both the patients who are receiving the drug and the clinicians drawing samples. Due to instability in plasma (t1/2  = 4 min), immediate stabilization of 4OHCP with phenylhydrazine is necessary upon sample draw. After stabilization, 4OHCP and the stable CP prodrug concentrations can be analytically measured to elucidate the pharmacokinetics, including half-life and exposure parameters (Cmax and AUC)...
April 10, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Qiong Lou, Jun Wen, Yuanying Jiang, Jinghua Huang, Guorong Fan, Fenyun Song
A reliable liquid chromatography/tandem mass (LC-MS/MS) method was developed for quantitation of 3', 4'-dimethoxy flavonol-3-β-d-glucopyranoside (DF3G) in rat plasma using pantoprazole as the internal standard. An Agilent C18 column (100 mm × 3 mm, 3.5 μm) was performed for chromatographic separation with the mobile phase composed of methanol-water (containing formic acid) at a flow rate of 0.4 mL/min. A triple-quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source in positive ion mode was applied to quantitative analysis by multiple reacting monitoring (MRM)...
April 7, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Julien Crepier, Agnès Le Masle, Nadège Charon, Florian Albrieux, Pascal Duchene, Sabine Heinisch
Extensive characterization of complex mixtures requires the combination of powerful analytical techniques. A Supercritical Fluid Chromatography (SFC) method was previously developed, for the specific case of fast pyrolysis bio oils, as an alternative to gas chromatography (GC and GC × GC) or liquid chromatography (LC and LC × LC), both separation methods being generally used prior to mass spectrometry (MS) for the characterization of such complex matrices. In this study we investigated the potential of SFC hyphenated to high resolution mass spectrometry (SFC-HRMS) for this characterization using Negative ion Atmospheric Pressure Chemical ionization ((-)APCI) for the ionization source...
April 7, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Lin-Yang Cheng, Chun-Lin Chen, Yueh-Hsiung Kuo, Tsung-Hsien Chang, I-Wei Lin, Shih-Wei Wang, Mei-Ing Chung, Jih-Jung Chen
A new polyprenylated polycyclic acylphloroglucinol, garcimultiflorone K (1), has been isolated from the stems of Garcinia multiflora, together with two known compounds, garcimultiflorone A (2) and garcimultiflorone B (3). The structure of new compound 1 was determined through spectroscopic methods including 1D and 2D NMR and MS analyses. The anti-angiogenic and anti-cancer effects of compounds 1-3 were evaluated in human endothelial progenitor cells (EPCs) and cancer cells. Of these, garcimultiflorone K (1) displayed the most potent anti-angiogenic property by suppressing cell growth and tube formation of EPCs...
April 6, 2018: Bioorganic & Medicinal Chemistry Letters
Jaakko Haverinen, Minna Hassinen, Hanna Korajoki, Matti Vornanen
Na+ channel α-subunit composition of the zebrafish heart and electrophysiological properties of Na+ current (INa ) of zebrafish ventricular myocytes were examined. Eight Na+ channel α-subunits were expressed in both atrium and ventricle of the zebrafish heart. Nav 1.5Lb, an orthologue to the human Nav 1.5, was clearly the predominant isoform in both chambers representing 65.2 ± 4.1% and 83.1 ± 2.1% of all Na+ channel transcripts in atrium and ventricle, respectively. Nav 1.4b, an orthologue to human Nav 1...
April 12, 2018: Progress in Biophysics and Molecular Biology
Jun Yan, Clay M Winterford, Vibeke S Catts, Betty K Pat, Michael P Pender, Pamela A McCombe, Judith M Greer
The NF-κB signalling pathway plays an important role in controlling cellular immune responses, inflammation and apoptosis. In multiple sclerosis (MS), there is evidence of dysregulation of NF-κB signalling in patients with a relapsing-remitting disease course, but thus far there is little information on whether it is also dysregulated in patients with progressive disease. We hypothesised that patients with progressive MS would have more activation of NF-κB than relapsing-remitting MS patients. Using several different methods, we showed that there was more nuclear translocation of p65 in cells from progressive MS patients, particularly in T cells and monocytes...
April 6, 2018: Journal of Neuroimmunology
Z Yousefi, M Kolahi, A Majd, P Jonoubi
Cadmium is an important environmental pollutant which genetically, physiologically and biochemically affects the cell. Phytochelatins (PC) are involved in one of the phytoremediation mechanisms, in which they are able to bind heavy metals, such as cadmium. The objective of this study was to evaluate morphometric, antioxidant enzyme activity, and SoPCS gene expression in sugarcane growing under cadmium stress. After propagation, samples were cultured in triplicate for 14 days in modified MS medium containing CdCl2 (100, 250, 500 µmol)...
April 12, 2018: Ecotoxicology and Environmental Safety
Minghua Zhu, Hongxia Zhao, Deming Xia, Juan Du, Huaijun Xie, Jingwen Chen
An accelerated solvent extraction (ASE) with in-cell clean-up method coupled to ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to determine 21 antibiotics in sea cucumber. The analytes include 10 sulfonamides, 4 fluoroquinolones, 3 amphenicols, 2 beta-lactams, 1 lincosamide and trimethoprim. Optimal parameters of ASE method were obtained at 80 °C, 1 static cycle of 5 min with methanol/acetonitrile (1/1, v/v) using 2 g of C18 as adsorbent. Recoveries at 50...
August 30, 2018: Food Chemistry
Anna Dawiec-Liśniewska, Antoni Szumny, Daria Podstawczyk, Anna Witek-Krowiak
The first article of a two-article series presents pervaporation (PV) of volatile aroma compounds from fruit juice hydrolates (plum, apple, blackcurrant and cherry). The purpose of this research was to evaluate the effectiveness of fruit juice hydrolates separation on a laboratory scale (inert gas flow pervaporation system) and semi-technical (vacuum pervaporation system) by means of pervaporation. To create precise matrices for hydrolates before and after pervaporation for each of the separated systems, solid phase microextraction (SPME) technique and the gas chromatography-mass spectrometry (GC-MS) was applied...
August 30, 2018: Food Chemistry
Dongwen Hu, Yang Xu, Jiahong Xie, Chongde Sun, Xiaodong Zheng, Wei Chen
This research aimed to investigate the protective effects of a new mulberry cultivar J33 with simulated gastrointestinal digestion against palmitic acid (PA)-induced lipotoxicity. LC-MS analysis revealed that the contents of four flavonoid glycosides (quercetin rhamnosylhexoside hexoside, quercetin rhamnosylhexoside, quercetin hexoside, kaempferol rhamnosylhexoside) increased after digestion. Besides, mulberry digest (MBD) at 0.5-2 mg/mL significantly reduced PA-induced lipotoxicity in human hepatocytes, while mulberry extract without digestion (MBE) showed no protection...
August 30, 2018: Food Chemistry
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