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https://www.readbyqxmd.com/read/28748667/a-model-of-genital-herpes-simplex-virus-type-1-infection-in-rhesus-macaques
#1
Meropi Aravantinou, Ines Frank, Geraldine Arrode-Bruses, Moriah Szpara, Brooke Grasperge, James Blanchard, Agegnehu Gettie, Nina Derby, Elena Martinelli
BACKGROUND: Although HSV-2 is the major cause of genital lesions, HSV-1 accounts for half of new cases in developed countries. METHODS: Three healthy SHIV-SF162P3-infected Indian rhesus macaques were inoculated with 4×10(8) pfu of HSV-1 twice, with the second inoculation performed after the vaginal mucosa was gently abraded with a cytobrush. RESULTS: HSV-1 DNA was detected in vaginal swabs 5 days after the second but not the first inoculation in all three macaques...
August 2017: Journal of Medical Primatology
https://www.readbyqxmd.com/read/28748212/genetically-engineered-multilineage-differentiating-stress-enduring-cells-as-cellular-vehicles-against-malignant-gliomas
#2
Tomohiro Yamasaki, Shohei Wakao, Hiroshi Kawaji, Shinichiro Koizumi, Tetsuro Sameshima, Mari Dezawa, Hiroki Namba
Malignant glioma, the most common malignant brain tumor in adults, is difficult to treat due to its aggressive invasive nature. Enzyme/prodrug suicide gene therapy based on the herpes simplex virus thymidine kinase (HSVtk)/ganciclovir (GCV) system is an efficient strategy for treating malignant gliomas. In the present study, we evaluated treatment with multilineage-differentiating stress-enduring (Muse) cells, which are endogenous non-tumorigenic pluripotent-like stem cells that are easily collectable from the bone marrow as SSEA-3(+) cells, as carriers of the HSVtk gene...
September 15, 2017: Molecular Therapy Oncolytics
https://www.readbyqxmd.com/read/28747509/a-domain-of-herpes-simplex-virus-pul33-required-to-release-monomeric-viral-genomes-from-cleaved-concatameric-dna
#3
Kui Yang, Xiaoqun Dang, Joel D Baines
Monomeric herpesvirus DNA is cleaved from concatamers and inserted into preformed capsids through the actions of the viral terminase. The terminase of herpes simplex virus (HSV) is composed of three subunits encoded by UL15, UL28, and UL33. The UL33 encoded protein (pUL33) interacts with pUL28 but its precise role in the DNA cleavage and packaging reaction is unclear. To investigate the function of pUL33 we generated a panel of recombinant viruses with either deletions or substitutions in the most conserved regions of UL33 using a bacterial artificial chromosome system...
July 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28747200/rubella-herpes-simplex-virus-type-2-and-preeclampsia
#4
Shimos A Alshareef, Ahmed M Eltom, Abubakr M Nasr, Hamdan Z Hamdan, Ishag Adam
BACKGROUND: Preeclampsia is a major health problem. Although, the pathophysiology of preeclampsia is not fully understood, there are recent studies on association between infections and preeclampsia. OBJECTIVE: The aim of the present study was to investigate the association between maternal seropositivity of rubella, Herpes simplex virus type 2 (HSV-2) and preeclampsia. METHOD: A case -controls study (90 women in each arm) was conducted at Saad Abualila Maternity Hospital, Khartoum, Sudan...
July 26, 2017: Virology Journal
https://www.readbyqxmd.com/read/28745327/seminal-vesicle-fluid-increases-the-efficacy-of-intravaginal-hsv-2-vaccination
#5
A Varese, F Remes Lenicov, M Gonzalez Prinz, A Paletta, G Ernst, C Maeto, A Merlotti, J Sabatte, S Símula, M P Holgado, E Dantas, J Geffner, A Ceballos
Once considered merely as a vehicle for spermatozoa, it is now clear that seminal plasma (SP) induces a variety of biological actions on the female reproductive tissues able to modulate the immune response against paternal antigens. To our knowledge, the influence of SP on the immune response against sexually transmitted pathogens has not been yet evaluated. We here analyzed whether the seminal vesicle fluid (SVF), which contributes almost 60% of the SP volume in mice, could modulate the immune response against herpes simplex virus type 2 (HSV-2)...
July 26, 2017: Mucosal Immunology
https://www.readbyqxmd.com/read/28744639/expression-of-ifn-inducible-genes-with-antiviral-function-oas1-and-mx1-in-health-and-under-conditions-of-recurrent-herpes-simplex-infection
#6
A V Karaulov, A E Shulzhenko, A V Karsonova
We studied the expression of IFN-inducible genes OAS1 and Mx1 in lysates of peripheral blood mononuclear cells from patients suffering from recurrent Herpes simplex infections in comparison with healthy people. To induce the expression of the studied genes, blood mononuclears were incubated with recombinant IFN-α2b in concentrations of 1, 10, and 100 U/ml for 3 h and then the content of the studied transcripts was evaluated. Relative expression of OAS1 and Mx1 in patients with recurrent forms of Herpes simplex both during the acute stage and clinical remission did not differ significantly from that in healthy people after stimulation with IFN-α2b in a concentration of 1 U/ml and in higher concentrations (10 and 100 U/ml)...
July 25, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28743747/the-ul8-subunit-of-the-helicase-primase-complex-of-herpes-simplex-virus-promotes-dna-annealing-and-has-a-high-affinity-for-replication-forks
#7
Oya Bermek, Sandra K Weller, Jack D Griffith
During lytic infection, herpes simplex virus (HSV) DNA is replicated by a mechanism involving DNA recombination. For instance, replication of the HSV-1 genome produces X- and Y-branched structures, reminiscent of recombination intermediates. HSV-1's replication machinery includes a trimeric helicase/primase is composed of helicase (UL5) and primase (UL52) subunits and a third subunit, UL8. UL8 has been reported to stimulate the helicase and primase activities of the complex in the presence of ICP8, an HSV-1 protein that functions as an annealase, a protein that binds complementary single-stranded (ss)DNA and facilitates its annealing to duplex DNA...
July 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28743741/cdk9-and-spt5-are-specifically-required-for-expression-of-herpes-simplex-virus-1-replication-dependent-late-genes
#8
Zhiyuan Zhao, Ka-Wei Tang, Isabella Muylaert, Tore Samuelsson, Per Elias
DNA replication greatly enhances expression of the Herpes simplex virus 1 (HSV-1) γ2 late genes by still unknown mechanisms. Here we demonstrate that 5,6-dichloro-1-β--D-ribofuranosylbenzimidazole (DRB), an inhibitor of CDK9, suppresses expression of γ2 late genes with an IC50 of 5 μM, which is at least ten times lower than the IC50 required for inhibition of expression of early genes. The effect of DRB could not be explained by inhibition of DNA replication per se or loading of RNA polymerase II to late promoters and subsequent reduction of transcription...
July 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28739437/short-term-exercise-training-enhances-cell-mediated-responses-to-hsv-1-vaccine-in-mice
#9
Molanouri Shamsi M, Najedi Sh, Hassan Zm, Isanejad A, Mahdavi M
Exercise (with appropriate intensity and duration) is a natural modulator of immune responses and may be useful to increase the vaccine response towards antigen. According to the fact that rural area responding butter than urbon area to vaccine protocol, this study was undertaken to test the hypothesis that short term exercise training as an adjuvant for antigen such as herpes simplex virus type 1 (HSV-1) in animal models. Mice with/without access to short term exercise training were immunized intramuscularly with inactivated KOS strain of HSV-1...
July 21, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28737006/unrecognized-viral-infections-and-chromosome-abnormalities-as-a-cause-of-fetal-death-examination-with-fluorescence-in%C3%A2-situ-hybridization-immunohistochemistry-and-polymerase-chain-reaction
#10
Bente Ediassen Opsjøn, Svein Arne Nordbø, Christina Vogt
Fifteen to 50% of fetal deaths remain unexplained after post-mortem examination depending on inclusion criteria and classification systems. Our aim was to examine a selection of unexplained fetal deaths in order to investigate whether any common chromosome aberrations or viral infections were present. Reports from 351 fetal autopsies performed at the Department of Pathology and Medical Genetics at St. Olavs University Hospital from 2001 through 2010 were reviewed. Of these, 105 fetal deaths were classified as unexplained...
July 24, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28736976/living-donor-liver-transplantation-for-neonatal-fulminant-hepatitis-due-to-herpes-simplex-virus-infection
#11
Rodrigo Vincenzi, Eduardo A Fonseca, Karina M O Roda, Gilda Porta, Helry L Candido, Marcel R Benavides, Katia R M Leite, Rogerio C Afonso, Plinio Turine-Neto, Cristiane M F Ribeiro, Paulo Chapchap, João Seda-Neto
Although rare, ALF caused by disseminated HSV infection is associated with high mortality in the neonatal population. This condition is often diagnosed relatively late due to the absence of specific signs. We present a case involving a neonate with ALF submitted to living donor liver transplantation without a prior diagnosis. The patient had no skin or mucosal lesions, and IgM serology was negative for HSV-1 and HSV-2. Immunohistochemical staining of the liver explant was positive for herpes virus infection, and the patient subsequently received antiviral drug treatment, with a good outcome...
July 23, 2017: Pediatric Transplantation
https://www.readbyqxmd.com/read/28735999/results-of-real-time-multiplex-polymerase-chain-reaction-assay-in-renal-transplant-recipients-with-sterile-pyuria
#12
M Sarier, M Demir, S Goktas, I Duman, M Buyukkinaci, Y Yuksel, S Tekin, A H Yavuz, A Sengul
Urinary tract infections are a major cause of morbidity and hospitalization after renal transplantation. Patients treated with immunosuppressive drugs suffer not only from common uropathogens but also from opportunistic infections caused by unusual uropathogens. Sterile pyuria is associated with numerous infectious agents including viruses, fungi, and atypical or fastidious organisms. The objective of this study was to investigate the pathogens using real-time multiplex polymerase chain reaction (rtMPCR) assay in sterile pyuria of renal transplant recipients...
July 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28734843/a-high-resolution-melting-hrm-technology-based-assay-for-cost-efficient-clinical-detection-and-genotyping-of-herpes-simplex-virus-hsv-1-and-hsv-2
#13
M Lieveld, A Carregosa, I Benoy, N Redzic, M Berth, D Vanden Broeck
Genital herpes can be caused by two very similar viruses, herpes simplex virus (HSV)-1 or HSV-2. These two HSV types cannot be distinguished clinically, but genotyping is recommended in the first-episodes of genital herpes to guide counselling and management. Quantitative polymerase chain reaction (qPCR) is the preferred diagnostic method for HSV typing. However, commercial qPCR methods use expensive fluorescent labeled probes for detection. Furthermore, most low-cost methods are not able to differentiate between HSV-1 and -2...
July 19, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28732069/saccharomyces-boulardii-cncm-i-745-supplementation-reduces-gastrointestinal-dysfunction-in-an-animal-model-of-ibs
#14
Paola Brun, Melania Scarpa, Chiara Marchiori, Gloria Sarasin, Valentina Caputi, Andrea Porzionato, Maria Cecilia Giron, Giorgio Palù, Ignazio Castagliuolo
BACKGROUND: We evaluated the effect of Saccharomyces boulardii CNCM I-745 on intestinal neuromuscular anomalies in an IBS-type mouse model of gastrointestinal motor dysfunctions elicited by Herpes Simplex Virus type 1 (HSV-1) exposure. METHODS: Mice were inoculated intranasally with HSV-1 (102 PFU) or vehicle at time 0 and 4 weeks later by the intragastric (IG) route (108 PFU). Six weeks after IG inoculum, mice were randomly allocated to receive oral gavage with either S...
2017: PloS One
https://www.readbyqxmd.com/read/28729548/multiplex-polymerase-chain-reaction-for-pathogen-detection-in-donor-recipient-corneal-transplant-tissue-and-donor-storage-solution
#15
Takehiro Hariya, Kazuichi Maruyama, Sunao Sugita, Masayo Takahashi, Shunji Yokokura, Kota Sato, Yasuhiro Tomaru, Norio Shimizu, Toru Nakazawa
Corneal transplantation is a safe, reliable method of restoring visual acuity in patients with corneal disorders. Although it has a very high success rate, rejection can still occur, especially if the site is infected. Therefore, seeking to find better ways to manage infection risk, this study investigated a new technique, based on multiplex polymerase chain reaction (mPCR), to identify pathogens, including viruses, bacteria, and fungi, in corneal transplantation recipient sites, donor corneas and the donor cornea storage solution...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729112/no-208-guidelines-for-the-management-of-herpes-simplex-virus-in-pregnancy
#16
Deborah M Money, Marc Steben
OBJECTIVE: To provide recommendations for the management of genital herpes infection in women who want to get pregnant or are pregnant and for the management of genital herpes in pregnancy and strategies to prevent transmission to the infant. OUTCOMES: More effective management of complications of genital herpes in pregnancy and prevention of transmission of genital herpes from mother to infant. EVIDENCE: Medline was searched for articles published in French or English related to genital herpes and pregnancy...
August 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28728870/outpatient-dermatology-consultation-impacts-the-diagnosis-and-management-of-pediatric-oncology-patients-a-retrospective-study
#17
Hannah Song, Sarah N Robinson, Jennifer T Huang
BACKGROUND: The impact of dermatology consultation on the care of children with oncologic conditions is unknown. OBJECTIVE: To review outpatient dermatology visits and the resulting impact on diagnosis and management of pediatric oncology patients. METHOD: Retrospective review of pediatric oncology patients with outpatient dermatology visits at a tertiary care center from 2008 to 2015. RESULTS: The most common dermatologic diagnoses in 516 patients were skin infections (21...
July 17, 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28727740/genetically-engineered-suicide-gene-in-mesenchymal-stem-cells-using-a-tet-on-system-for-anaplastic-thyroid-cancer
#18
Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
Anaplastic thyroid cancer (ATC) is the most aggressive malignancy of the thyroid, during which undifferentiated tumors arise from the thyroid follicular epithelium. ATC has a very poor prognosis due to its aggressive behavior and poor response to conventional therapies. Gene-directed enzyme/prodrug therapy using genetically engineered mesenchymal stromal cells (MSC) is a promising therapeutic strategy. The doxycycline (DOX)-controlled Tet inducible system is the most widely utilized regulatory system and could be a useful tool for therapeutic gene-based therapies...
2017: PloS One
https://www.readbyqxmd.com/read/28726789/predominant-area-of-brain-lesions-in-neonates-with-herpes-simplex-encephalitis
#19
H Kidokoro, L S de Vries, C Ogawa, Y Ito, A Ohno, F Groenendaal, S Saitoh, A Okumura, Y Ito, J Natsume
OBJECTIVE: Nonspecific manifestations and a varied distribution of brain lesions can delay the diagnosis of herpes simplex encephalitis (HSE) in neonates. The aim of this study was to report predominant brain lesions in neonatal HSE, and then to investigate the association between pattern of predominant brain lesions, clinical variables and neurodevelopmental outcome. STUDY DESIGN: A multicenter retrospective study was performed in neonates diagnosed with HSE between 2009 and 2014...
July 20, 2017: Journal of Perinatology: Official Journal of the California Perinatal Association
https://www.readbyqxmd.com/read/28723708/subclinical-herpesvirus-shedding-among-hiv-1-infected-men-on-antiretroviral-therapy
#20
Arcadio Agudelo-Hernandez, Yue Chen, Arlene Bullotta, William G Buchanan, Cynthia R Klamar-Blain, Luann Borowski, Sharon A Riddler, Charles R Rinaldo, Bernard Jc Macatangay
DESIGN: We evaluated the subclinical shedding of 6 different herpesviruses in antiretroviral drug-treated HIV(+) men who have sex with men (MSM), and determined how this is associated with markers of inflammation and immune activation. METHODS: We obtained blood, semen, throat washing, urine, and stool from 15 antiretroviral-treated HIV-1-infected MSM with CD4 T cell reconstitution, and 12 age-matched HIV(-) MSM from the Multicenter AIDS Cohort Study at 4 timepoints over 24 weeks to measure DNA levels of cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus 1 and 2, human herpesvirus 6 (HHV6), and HHV8...
July 18, 2017: AIDS
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