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https://www.readbyqxmd.com/read/28231564/mating-of-2-laboratory-saccharomyces-cerevisiae-strains-resulted-in-enhanced-production-of-2-phenylethanol-by-biotransformation-of-l-phenylalanine
#1
Jolanta Mierzejewska, Aleksandra Tymoszewska, Karolina Chreptowicz, Kamil Krol
2-Phenylethanol (2-PE) is an aromatic alcohol with a rosy scent which is widely used in the food, fragrance, and cosmetic industries. Promising sources of natural 2-PE are microorganisms, especially yeasts, which can produce 2-PE by biosynthesis and biotransformation. Thus, the first challenging goal in the development of biotechnological production of 2-PE is searching for highly productive yeast strains. In the present work, 5 laboratory Saccharomyces cerevisiae strains were tested for the production of 2-PE...
February 24, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28231267/accuracy-of-optical-biometry-combined-with-placido-disc-corneal-topography-for-intraocular-lens-power-calculation
#2
Giacomo Savini, Kenneth J Hoffer, Piero Barboni, Nicole Balducci, Domenico Schiano-Lomoriello, Pietro Ducoli
PURPOSE: To investigate the accuracy of a new optical biometer for intraocular lens (IOL) power calculation in eyes undergoing cataract surgery. METHODS: Consecutive eyes of patients undergoing cataract surgery with the same IOL model were enrolled in a prospective cohort study. Axial length (AL) and corneal power were measured with an optical biometer based on optical low-coherence interferometry and Placido-disc corneal topography. IOL power was calculated with the Hoffer Q, Holladay 1 and SRK/T formulas...
2017: PloS One
https://www.readbyqxmd.com/read/28231148/potential-use-of-gamma-irradiated-ethnic-ready-to-eat-foods-to-improve-the-nutritional-status-of-landslide-victims
#3
REVIEW
Zubaidah Irawati Koenari, Carmen M Siagian, Bona Simanungkalit, Asti Nilatany, Indra Mustika Pratama, Deudeu Lasmawati, Cecep M Nurcahya
The safety and high quality of ethnic ready-to-eat foods as a source of nourishment and food supplies chain to the remote areas become particularly important. Consuming healthy and balanced nutritional foods means eating better quality foods in proper quantities. Such foods can be developed through a preservation technique by using ionizing radiation. Although implementation of the technology for certain foodstuffs has been implemented in some countries, application of the process to a complete set of meals for selected target groups is still very limited...
July 26, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28231022/caution-regarding-catheter-directed-thrombolysis-cteph-mistaken-for-acute-submassive-pe
#4
Timothy M Fernandes, Victor G Pretorius, Nick H Kim
No abstract text is available yet for this article.
February 23, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/28228575/risk-of-pulmonary-embolism-after-cerebral-venous-thrombosis
#5
Ava L Liberman, Alexander E Merkler, Gino Gialdini, Steven R Messé, Michael P Lerario, Santosh B Murthy, Hooman Kamel, Babak B Navi
BACKGROUND AND PURPOSE: Cerebral vein thrombosis (CVT) is a type of venous thromboembolism. Whether the risk of pulmonary embolism (PE) after CVT is similar to the risk after deep venous thrombosis (DVT) is unknown. METHODS: We performed a retrospective cohort study using administrative data from all emergency department visits and hospitalizations in California, New York, and Florida from 2005 to 2013. We identified patients with CVT or DVT and the outcome of PE using previously validated International Classification of Diseases, Ninth Revision, Clinical Modification codes...
February 22, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28228116/examination-of-motor-skill-competency-in-students-evidence-based-physical-education-curriculum
#6
Weiyun Chen, Austin Hammond-Bennett, Andrew Hypnar
BACKGROUND: Researchers found that children with a competent level of motor skill performance are more likely to be physically active. This study examined how well K-1 students demonstrated motor skill competency in relation to Physical Education Content Standard 1. METHODS: Participants were K-1 grade students (N = 1,223-1,588; boys = 568-857; girls = 526-695; Mean age = 5.5 yrs old) who were enrolled in nine elementary schools. The K-1 students' motor skill competency in running, weight transferring, hand dribbling, and underhand catching skills was assessed using four PE Metrics skill assessment rubrics in the intervention year 1 and year 2, respectively...
February 23, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28227934/effect-of-embedding-dimension-on-complexity-measures-in-identifying-arrhythmia
#7
Radhagayathri K Udhayakumar, Chandan Karmakar, Peng Li, Marimuthu Palaniswami, Radhagayathri K Udhayakumar, Chandan Karmakar, Peng Li, Marimuthu Palaniswami, Marimuthu Palaniswami, Radhagayathri K U Udhayakumar, Peng Li, Chandan Karmakar
Entropy measures like Approximate entropy (ApEn) and Sample entropy (SampEn) are well established tools to analyze Heart Rate Variability (HRV) data. Critical parameters involved in these computations namely embedding dimension m and tolerance r are in most cases assumed to be 2 and 0.2*signal SD (standard devaition) respectively. Such assumptions do not work fairly across data sets and thus create misleading results in many cases. Problems with r have been addressed with the advent of newer entropy measures like Permutation entropy (PE), Fuzzy entropy (FuzzyEn) and Distribution entropy (DistEn) that simply eliminate, modify or replace r from calculations...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227468/patient-emulator-a-tool-for-testing-mechanical-ventilation-therapies
#8
Nikolaos Karamolegkos, Antonio Albanese, Fernando Isaza, Nicolas W Chbat, Nikolaos Karamolegkos, Antonio Albanese, Fernando Isaza, Nicolas W Chbat, Nikolaos Karamolegkos, Antonio Albanese, Nicolas W Chbat, Fernando Isaza
Several modes of mechanical ventilation are clinically available. The differences among them in terms of efficacy and patient outcomes are not clear yet. Testing and comparison of mechanical ventilation modes via human or animal trials is a very challenging and costly process. In this paper, we present the patient emulator (PE), a novel system that can be used as a platform for in-silico testing of mechanical ventilation therapies. The system is based on a large-scale integrated mathematical model of the human cardiopulmonary system interfaced with a physical ventilator via a controlled piston-cylinder actuator...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227129/modeling-of-pharyngoesophageal-segment-during-tracheoesophageal-phonation-in-total-laryngectomy-patients-with-preliminary-validation
#9
Teng Zhang, Siwei Bai, Ian Cook, Michal Szczesniak, Julia Maclean, Socrates Dokos, Teng Zhang, Siwei Bai, Ian Cook, Michal Szczesniak, Julia Maclean, Socrates Dokos, Siwei Bai, Michal Szczesniak, Socrates Dokos, Ian Cook, Julia Maclean, Teng Zhang
For patients with large or recurrent laryngeal cancer, total laryngectomy surgery is usually performed. In the absence of a larynx, tracheoesophageal (TE) voice is the gold standard for alaryngeal phonation. The mechanisms underlying TE voice are unknown, but pharyngoesophageal (PE) segment vibration has been shown to play a key role. The aim of this study was to systematically explore the mechanics of PE segment vibration during TE phonation. A 2D axisymmetric finite-element model with two domains representing the air and PE wall was developed...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28224261/determination-of-glucose-exchange-rates-and-permeability-of-erythrocyte-membrane-in-preeclampsia-and-subsequent-oxidative-stress-related-protein-damage-using-dynamic-19-f-nmr
#10
Elizabeth Dickinson, John R P Arnold, Julie Fisher
The cause of the pregnancy condition preeclampsia (PE) is thought to be endothelial dysfunction caused by oxidative stress. As abnormal glucose tolerance has also been associated with PE, we use a fluorinated-mimic of this metabolite to establish whether any oxidative damage to lipids and proteins in the erythrocyte membrane has increased cell membrane permeability. Data were acquired using (19)F Dynamic-NMR (DNMR) to measure exchange of 3-fluoro-3-deoxyglucose (3-FDG) across the membrane of erythrocytes from 10 pregnant women (5 healthy control women, and 5 from women suffering from PE)...
February 21, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28224250/abscisic-acid-sucrose-and-auxin-coordinately-regulate-berry-ripening-process-of-the-fujiminori-grape
#11
Haifeng Jia, Zhenqiang Xie, Chen Wang, Lingfei Shangguan, Ning Qian, Mengjie Cui, Zhongjie Liu, Ting Zheng, Mengqi Wang, Jinggui Fang
The aim of this study was to examine the effect of abscisic acid (ABA), sucrose, and auxin on grape fruit development and to assess the mechanism of these three factors on the grape fruit ripening process. Different concentrations of ABA, sucrose, and auxin were used to treat the grape fruit, and the ripening-related indices, such as physiological and molecular level parameters, were analyzed. The activity of BG protein activity was analyzed during the fruit development. Sucrose, ABA, and auxin influenced the grape fruit sugar accumulation in different ways, as well as the volatile compounds, anthocyanin content, and fruit firmness...
February 21, 2017: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/28224155/metabonomic-analysis-of-quercetin-against-the-toxicity-of-acrylamide-in-rat-urine
#12
Wei Bao, Can Cao, Siqi Li, Lu Bo, Meiyan Zhang, Xiujuan Zhao, Ying Liu, Changhao Sun
This research aims to determine whether quercetin has protective effects against the toxicity of acrylamide (AA) using metabonomic technology. Randomly, the rats were assigned into a control group, AA treatment group, quercetin treatment group and quercetin plus AA treatment group. Quercetin and AA were administered to rats daily via gavage and drinking water for 16 weeks, respectively. To detect the metabonomic profiles of urine, ultra-performance liquid chromatography/mass spectrometry was used. A total of 15 metabolites, including biomarkers of AA exposure (GAMA, AAMA, and iso-AAMA) and quercetin exposure (quercetin and isorhamnetin), were identified...
February 22, 2017: Food & Function
https://www.readbyqxmd.com/read/28223967/secretome-prediction-of-two-m-tuberculosis-clinical-isolates-reveals-their-high-antigenic-density-and-potential-drug-targets
#13
Fernanda Cornejo-Granados, Zyanya L Zatarain-Barrón, Vito A Cantu-Robles, Alfredo Mendoza-Vargas, Camilo Molina-Romero, Filiberto Sánchez, Luis Del Pozo-Yauner, Rogelio Hernández-Pando, Adrián Ochoa-Leyva
The Excreted/Secreted (ES) proteins play important roles during Mycobacterium tuberculosis invasion, virulence, and survival inside the host and they are a major source of immunogenic proteins. However, the molecular complexity of the bacillus cell wall has made difficult the experimental isolation of the total bacterial ES proteins. Here, we reported the genomes of two Beijing genotype M. tuberculosis clinical isolates obtained from patients from Vietnam (isolate 46) and South Africa (isolate 48). We developed a bioinformatics pipeline to predict their secretomes and observed that ~12% of the genome-encoded proteins are ES, being PE, PE-PGRS, and PPE the most abundant protein domains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28222922/biomass-production-and-carbon-sequestration-of-a-short-rotation-forest-with-different-poplar-clones-in-northwest-china
#14
Yan Meifang, Wang Lu, Ren Honghui, Zhang Xinshi
Short Rotation Forestry (SRF) is of interest as producers of biomass for bio-energy, but also as carbon (C) sinks to mitigate CO2 emission. To investigate biomass production and C sequestration of SRF, ecosystem C stock (including C stored in tree biomass, litter and soil), NPP (net primary productivity), heterotrophic respiration (Rh) and NEP (net ecosystem productivity) of three poplar clone plantations were estimated by repeated field sampling in northwest China. Ecosystem C stock (105.62MgCha(-1)) was significantly lower in PB (P...
February 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28222709/combined-application-versus-topical-and-intravenous-application-of-tranexamic-acid-following-primary-total-hip-arthroplasty-a-meta-analysis
#15
Pei Zhang, Yuan Liang, Pengtao Chen, Yongchao Fang, Jinshan He, Jingcheng Wang
BACKGROUND: The use of intravenous (IV) or topical tranexamic acid (TXA) in total hip arthroplasty has been proven to be effective and safe in total hip arthroplasty. However, which of these two administration routes is better has not been determined. The combined administration of TXA has been used in total knee arthroplasty with satisfactory results. We hypothesized that combined application of TXA may be the most effective way without increased rate of thrombotic events such as deep vein thrombosis (DVT) and pulmonary embolisms (PE) in patients subjected to primary total hip replacement (THA)...
February 21, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28222263/reaction-mechanism-of-covalent-modification-of-phosphatidylethanolamine-lipids-by-reactive-aldehydes-4-hydroxy-2-nonenal-and-4-oxo-2-nonenal
#16
Katarina Vazdar, Danijela Vojta, Davor Margetić, Mario Vazdar
4-hydroxy-2-nonenal (HNE) and 4-oxo-2-nonenal (ONE) are biologically important reactive aldehydes formed during oxidative stress in phospholipid bilayers. They are highly reactive species due to presence of several reaction centers and can react with amino acids in peptides and proteins, as well as phosphoethanolamine (PE) lipids, thus modifying their biological activity. The aim of this work is to study in a molecular detail the reactivity of HNE and ONE towards PE lipids in a simplified system containing only lipids and reactive aldehydes in dichloromethane as an inert solvent...
February 21, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28220329/disproportional-increase-of-pulmonary-embolism-in-young-females-in-germany-trends-from-2005-to-2014
#17
F Santosa, Ch Moerchel, Ch Berg, K Kröger
We analysed the number of pulmonary embolism (PE) in young females aged 10-39 years in the period from 2005 to 2014 in Germany to see any trends that possibly may reflect some influences of newer oral contraceptives. Detailed lists of all hospitalized cases with the principal diagnosis PE coded as I26 in the years 2005-2014 were provided by the Federal Statistical Office. In males, the absolute number of all hospitalized cases with the principal diagnosis PE increased from 16,066 in 2005 to 25,364 in 2014 (rate +57...
February 20, 2017: Journal of Thrombosis and Thrombolysis
https://www.readbyqxmd.com/read/28220203/acinetobacter-halotolerans-sp-nov-a-novel-halotolerant-alkalitolerant-and-hydrocarbon-degrading-bacterium-isolated-from-soil
#18
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
A novel aerobic, non-motile, halotolerant, alkalitolerant, hydrocarbon degrading, and rod shaped bacterium, designated strain R160(T), was isolated from soil in South Korea. Cells were Gram-staining-negative, catalase-positive, and oxidase-negative. This strain grew up to 7% of NaCl and in the pH range of 6-11 (optimum 7.0-10.0). The isolate degraded 51.7 ± 1.3% of hydrocarbon components (C-18, C-20, and C-22) and 45.8 ± 1.4% oil components (kerosene, diesel, and gasoline). Phylogenetic analysis based on 16 S rRNA gene sequences revealed that strain R160(T) formed a lineage within the genus Acinetobacter, and was closely related to 'Acinetobacter oleivorans' DR1(T) (97...
February 20, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28219782/characterization-of-phospholipid-nitroxidation-by-lc-ms-in-biomimetic-models-and-in-h9c2-myoblast-using-a-lipidomic-approach
#19
Tânia Melo, Pedro Domingues, Teresa M Ribeiro-Rodrigues, Henrique Girão, Marcela A Segundo, M Rosário M Domingues
Under nitroxidative stress conditions, lipids are prone to be modified by reaction with reactive nitrogen species (RNS) and different modifications were reported to occur in fatty acids. However, in the case of phospholipids (PL) studied under nitroxidative stress conditions, only nitroalkene derivatives of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), were reported when using both in vitro biomimetic conditions and in vivo model system of type 1 diabetes mellitus. Therefore, in order to further explore other nitroxidative modifications of PL, a biomimetic model of nitroxidation combined with liquid chromatography mass spectrometry (MS) and MS/MS approaches were used to characterize the nitrated and nitroxidized derivatives of PCs and PEs...
February 17, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28218957/cyclic-fatigue-resistance-of-a-novel-rotary-file-manufactured-using-controlled-memory-ni-ti-technology-compared-to-a-file-made-from-m-wire-file
#20
E Alshwaimi
AIM: To compare the cyclic fatigue properties of a novel file made using controlled memory NiTi technology with those of files made from M-wire. METHODOLOGY: Twelve files with similar cross-sectional geometry and tip size from each of the following groups were tested: Proflexendo made from CMT (PE; size 30 .04) (Nexden, Houston, Tx, USA), ProFile Vortex made from M-wire (PV; size 30 .04) (Dentsply Tulsa Dental, Tulsa, OK, USA), and ProTaper Universal made from regular alloy (PU; F3) (Dentsply Tulsa Dental)...
February 20, 2017: International Endodontic Journal
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