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Abc Transporter

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https://www.readbyqxmd.com/read/29684377/biphasic-modulation-of-camp-levels-by-the-contraceptive-nomegestrol-acetate-impact-on-p-glycoprotein-expression-and-activity-in-hepatic-cells
#1
Guillermo Nicolás Tocchetti, Camila Juliana Domínguez, Felipe Zecchinati, Maite Rocío Arana, María Laura Ruiz, Silvina Stella Maris Villanueva, Johanna Weiss, Aldo Domingo Mottino, Juan Pablo Rigalli
ABC transporters are key players in drug excretion with alterations in their expression and activity by therapeutic agents potentially leading to drug-drug interactions. The interaction potential of nomegestrol acetate (NMGA), a synthetic progestogen increasingly used as oral contraceptive, had never been explored. In this work we evaluated (1) the effect of NMGA on ABC transporters in the human hepatic cell line HepG2 and (2) the underlying molecular mechanism. NMGA (5, 50 and 500 nM) increased P-glycoprotein (P-gp) expression at both protein and mRNA levels and reduced intracellular calcein accumulation, indicating an increase also in transporter activity...
April 20, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29682513/lung-cancer-stem-cells-origin-characteristics-and-therapy
#2
REVIEW
D Prabavathy, Y Swarnalatha, Niveditha Ramadoss
Cancer stem cells (CSCs) have gained an increasing attention recently in cancer research. CSCs have ability to generate new tumor through their stem cell properties, essentially self-renewal potential and differentiation into multiple cell lineages. Extensive evidences report that CSCs are resistant to many conventional therapies and mediate tumor recurrence. CSCs of lung cancer are well recognized by their specific markers such as CD133, CD44, ABCG2 and ALDH1A1 together with the CSC characteristics including spheroid and colony formation...
2018: Stem Cell Investigation
https://www.readbyqxmd.com/read/29682213/pharmacogenetics-based-area-under-curve-model-can-predict-efficacy-and-adverse-events-from-axitinib-in-individual-patients-with-advanced-renal-cell-carcinoma
#3
Yoshiaki Yamamoto, Ryouichi Tsunedomi, Yusuke Fujita, Toru Otori, Mitsuyoshi Ohba, Yoshihisa Kawai, Hiroshi Hirata, Hiroaki Matsumoto, Jun Haginaka, Shigeo Suzuki, Rajvir Dahiya, Yoshihiko Hamamoto, Kenji Matsuyama, Shoichi Hazama, Hiroaki Nagano, Hideyasu Matsuyama
We investigated the relationship between axitinib pharmacogenetics and clinical efficacy/adverse events in advanced renal cell carcinoma (RCC) and established a model to predict clinical efficacy and adverse events using pharmacokinetic and gene polymorphisms related to drug metabolism and efflux in a phase II trial. We prospectively evaluated the area under the plasma concentration-time curve (AUC) of axitinib, objective response rate, and adverse events in 44 consecutive advanced RCC patients treated with axitinib...
March 30, 2018: Oncotarget
https://www.readbyqxmd.com/read/29681147/transcriptomic-analysis-of-xylan-oligosaccharides-utilization-systems-in-pediococcus-acidilactici-strain-bcc-1
#4
Zhao Lei, Yu Qin Wu, Wei Nie, Dafei Yin, Xiaonan Yin, Yuming Guo, Samuel Aggrey, Jianmin Yuan
Xylanoligosaccharides (XOS) is the hydrolysates of xylan. To compare the proliferation effect of XOS, glucose, fructooligosaccharides (FOS), xylose, XOS and a media without carbohydrate source (control) on Pediococcus acidilactici strain BCC-1. The de-novo sequencing of Pediococcus acidilactici strain BCC-1 was conducted, and the underlying mechanism of prebiotic xylooligosaccharide between xylose and XOS was revealed through transcriptomic analysis. Compared to FOS, glucose and xylose, XOS exhibits a good performance in promoting the fermentation of Pediococcus acidilactici BCC-1...
April 22, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29678880/structural-and-thermodynamic-insights-into-%C3%AE-1-2-glucooligosaccharide-capture-by-a-solute-binding-protein-in-listeria-innocua
#5
Koichi Abe, Naoki Sunagawa, Tohru Terada, Yuta Takahashi, Takatoshi Arakawa, Kiyohiko Igarashi, Masahiro Samejima, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima, Shinya Fushinobu
β-1,2-Glucans are bacterial carbohydrates that exist in cyclic or linear forms and play an important role in infections and symbioses involving Gram-negative bacteria. Although several β-1,2-glucan-associated enzymes have been characterized, little is known about how β-1,2-glucan and its shorter oligosaccharides (Sopn s) are captured and imported into the bacterial cell. Here, we report the biochemical and structural characteristics of Sopn -binding protein (SO-BP, Lin1841) associated with the ABC transporter from the Gram-positive bacterium Listeria innocua Calorimetric analysis revealed that SO-BP specifically binds to Sopn s with degree of polymerization of 3 or more, with Kd  values in the micromolar range...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678549/title-reversal-of-drug-resistance-by-planetary-ball-milled-pbm-nanoparticle-loaded-with-resveratrol-and-docetaxel-in-prostate-cancer
#6
Santosh Kumar Singh, James W Lillard, Rajesh Singh
The folate receptor (FR) is a valued target that is highly expressed in various cancers, which will expedite the development of ligand-receptor binding based cancer therapeutics. In the present investigation, through tissue microarray analysis, we report higher levels of folate receptor expression in prostate cancer (PCa) tissue derived from patients, which were minimal in normal tissue. For folate-receptor based targeted therapy of PCa, we generated novel planetary ball milled (PBM) nanoparticles (NPs) encapsulated with resveratrol (RES), and in combination with docetaxel (DTX) and conjugated with folic acid (FA) on the surface...
April 17, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29678468/the-s-layer-biogenesis-system-of-synechocystis-6803-role-of-sll1180-and-sll1181-e-coli-hlyb-and-hlyd-analogs-as-type-i-secretion-components-for-sll1951-export
#7
Rachna Agarwal, Julian P Whitelegge, Sanjay Saini, Amit Prakash Shrivastav
Multiple secretion pathways are known for export of protein(s) forming the S-layer in bacteria. The unicellular model cyanobacterium Synechocystis sp. strain PCC 6803 (hereafter S. 6803) also possesses a well-defined S-layer composed of Sll1951 protein. However, the mechanism of its secretion is not completely understood. In the present study, the putative T1SS (Type I secretion system) components, Sll1180 and Sll1181 [inner membrane ABC transporter and membrane fusion protein (MFP), respectively] were characterized for their role in Sll1951 secretion...
April 18, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29675872/identification-and-quantification-of-blood-brain-barrier-transporters-in-isolated-rat-brain-microvessels
#8
Hajar Al Feteisi, Zubida M Al-Majdoub, Brahim Achour, Narciso Couto, Amin Rostami-Hodjegan, Jill Barber
The blood-brain barrier (BBB) maintains brain homeostasis by tightly regulating the exchange of molecules with systemic circulation. It consists primarily of microvascular endothelial cells surrounded by astrocytic endfeet, pericytes, and microglia. Understanding the make-up of transporters in rat BBB is essential to the translation of pharmacological and toxicological observations into humans. In this study, experimental workflows are presented in which the optimization of (a) isolation of rat brain microvessels, (b) enrichment of endothelial cells, and (c) extraction and digestion of proteins were evaluated, followed by identification and quantification of BBB proteins...
April 20, 2018: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29673999/ribociclib-shows-potential-for-pharmacokinetic-drug-drug-interactions-being-a-substrate-of-abcb1-and-potent-inhibitor-of-abcb1-abcg2-and-cyp450-isoforms-in-vitro
#9
Ales Sorf, Jakub Hofman, Radim Kučera, Frantisek Staud, Martina Ceckova
Ribociclib is a novel cyclin-dependent kinase (CDK) 4 and 6 selective inhibitor that recently gained breakthrough therapy status and global approval for advanced breast cancer treatment. ATP-binding cassette (ABC) transporters may become a site of severe drug interactions and a mechanism of multidrug resistance (MDR) development. With respect to rapid progress of ribociclib in the clinical field, we aimed to identify its interactions with ABC transporters and cytochrome P450 (CYP) isoenzymes and evaluate its potential to overcome transporter-mediated MDR using established in vitro methods...
April 16, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29672568/rna-seq-transcriptome-analysis-of-amaranthus-palmeri-with-differential-tolerance-to-glufosinate-herbicide
#10
Reiofeli A Salas-Perez, Christopher A Saski, Rooksana E Noorai, Subodh K Srivastava, Amy L Lawton-Rauh, Robert L Nichols, Nilda Roma-Burgos
Amaranthus palmeri (Amaranthaceae) is a noxious weed in several agroecosystems and in some cases seriously threatens the sustainability of crop production in North America. Glyphosate-resistant Amaranthus species are widespread, prompting the use of alternatives to glyphosate such as glufosinate, in conjunction with glufosinate-resistant crop cultivars, to help control glyphosate-resistant weeds. An experiment was conducted to analyze the transcriptome of A. palmeri plants that survived exposure to 0.55 kg ha-1 glufosinate...
2018: PloS One
https://www.readbyqxmd.com/read/29670629/regulation-of-human-%C3%AE-%C3%AE-t-cells-by-btn3a1-protein-stability-and-atp-binding-cassette-transporters
#11
David A Rhodes, Hung-Chang Chen, James C Williamson, Alfred Hill, Jack Yuan, Sam Smith, Harriet Rhodes, John Trowsdale, Paul J Lehner, Thomas Herrmann, Matthias Eberl
Activation of human Vγ9/Vδ2 T cells by "phosphoantigens" (pAg), the microbial metabolite ( E )-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP) and the endogenous isoprenoid intermediate isopentenyl pyrophosphate, requires expression of butyrophilin BTN3A molecules by presenting cells. However, the precise mechanism of activation of Vγ9/Vδ2 T cells by BTN3A molecules remains elusive. It is not clear what conformation of the three BTN3A isoforms transmits activation signals nor how externally delivered pAg accesses the cytosolic B30...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29666229/drought-delays-development-of-the-sorghum-root-microbiome-and-enriches-for-monoderm-bacteria
#12
Ling Xu, Dan Naylor, Zhaobin Dong, Tuesday Simmons, Grady Pierroz, Kim K Hixson, Young-Mo Kim, Erika M Zink, Kristin M Engbrecht, Yi Wang, Cheng Gao, Stephanie DeGraaf, Mary A Madera, Julie A Sievert, Joy Hollingsworth, Devon Birdseye, Henrik V Scheller, Robert Hutmacher, Jeffery Dahlberg, Christer Jansson, John W Taylor, Peggy G Lemaux, Devin Coleman-Derr
Drought stress is a major obstacle to crop productivity, and the severity and frequency of drought are expected to increase in the coming century. Certain root-associated bacteria have been shown to mitigate the negative effects of drought stress on plant growth, and manipulation of the crop microbiome is an emerging strategy for overcoming drought stress in agricultural systems, yet the effect of drought on the development of the root microbiome is poorly understood. Through 16S rRNA amplicon and metatranscriptome sequencing, as well as root metabolomics, we demonstrate that drought delays the development of the early sorghum root microbiome and causes increased abundance and activity of monoderm bacteria, which lack an outer cell membrane and contain thick cell walls...
April 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29665654/hypericin-affects-cancer-side-populations-via-competitive-inhibition-of-bcrp
#13
Jana Vargová, Jaromír Mikeš, Rastislav Jendželovský, Lucia Mikešová, Barbora Kuchárová, Ľubomír Čulka, Radek Fedr, Ján Remšík, Karel Souček, Alois Kozubík, Peter Fedoročko
OBJECTIVE: Cancer stem-like cells (CSLCs) are considered a root of tumorigenicity and resistance. However, their identification remains challenging. The use of the side population (SP) assay as a credible marker of CSLCs remains controversial. The SP assay relies on the elevated activity of ABC transporters that, in turn, can be modulated by hypericin (HYP), a photosensitizer and bioactive compound of St. John's Wort (Hypericum perforatum), a popular over-the-counter antidepressant. Here we aimed to comprehensively characterize the SP phenotype of cancer cells and to determine the impact of HYP on these cells...
March 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29665404/efficacy-of-recombinant-protein-vaccines-for-protection-against-nocardia-seriolae-infection-in-the-largemouth-bass-micropterus-salmoides
#14
Ping-Yueh Ho, Yao-Chung Chen, Shun Maekawa, Hsiang-Hui Hu, An-Wei Tsai, Yung-Fu Chang, Pei-Chi Wang, Shih-Chu Chen
A reverse vaccinology-based survey of potent antigens associated with fish nocardiosis was conducted using the largemouth bass, Micropterus salmoides, with an aim to develop subunit vaccines. The antigens selected from the virulent strain Nocardia seriolae 961113 include the gene products of NGL2579 (GAPDH), NGL5701 (MMP), NGL4377 (OCTase), NGL4486 (ABC transporter), NGL3372 (LLE), NGL3388 (GHf10), NGL6627 (Antigen-85), NGL6696 (Esterase), and NGL6936 (CBP). These antigens were heterologously expressed in E...
April 14, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29662735/effect-of-superoxide-dismutase-and-manganese-on-superoxide-tolerance-in-lactobacillus-casei-strain-shirota-and-analysis-of-multiple-manganese-transporters
#15
Masaki Serata, Emi Yasuda, Tomoyuki Sako
The Lactobacillus casei / paracasei group accumulates a high level of manganese, which works to scavenge superoxide anions produced during aerobic growth. The genome of L. casei strain Shirota, however, also codes the gene for superoxide dismutase (SOD), sodA , which catalyzes the dismutation of superoxide anion into hydrogen peroxide and oxygen. We anticipated that the SOD and/or manganese may contribute to the aerobic growth of L. casei Shirota and tried to clarify how L. casei Shirota can eliminate the toxicity of superoxide anion...
2018: Bioscience of Microbiota, Food and Health
https://www.readbyqxmd.com/read/29660518/hoxb4-knockdown-enhances-the-cytotoxic-effect-of-paclitaxel-and-cisplatin-by-downregulating-abc-transporters-in-ovarian-cancer-cells
#16
Ying Li, Jingli Sun, Shaofeng Gao, Heping Hu, Pengmu Xie
Therapeutic effects of anti-cancer drugs for ovarian cancer were limited due to the rapid development of chemotherapy resistance. The aim of this study was to test whether knockdown of Homeobox B4 (HOXB4) enhanced the cytotoxic effect of paclitaxel and cisplatin in ovarian cancer cells. HOXB4 expressions at mRNA and protein levels were upregulated in Taxol-resistant A2780 (A2780/Taxol) and DDP-resistant SKOV-3 (SKOV-3/DDP) cells. HOXB4 knockdown enhanced the cytotoxic effects of Taxol and DDP in A2780/Taxol and SKOV-3/DDP cells, respectively...
April 13, 2018: Gene
https://www.readbyqxmd.com/read/29660159/cannabis-and-anti-cancer-drugs-societal-usage-and-expected-pharmacological-interactions-a-review
#17
REVIEW
Régis Bouquié, Guillaume Deslandes, Hélène Mazaré, Marion Cogné, Julien Mahé, Matthieu Grégoire, Pascale Jolliet
Cannabis is a plant that has been used for centuries to relieve a wide range of symptoms. Since the 1960s, interest in medical research into this plant has grown steadily. Already very popular for recreational use, a growing number of consumers not accustomed to using cannabis for psychoactive purposes, have begun to use it as an alternative or complement to mainstream pharmaceutical medicines. The principal unsubstantiated or "social" uses of cannabis are based mainly on data that is at best controversial, but usually not scientifically proven...
April 16, 2018: Fundamental & Clinical Pharmacology
https://www.readbyqxmd.com/read/29658612/let-7d-3p-is-associated-with-apoptosis-and-response-to-neoadjuvant-chemotherapy-in-ovarian-cancer
#18
Raúl García-Vázquez, Dolores Gallardo Rincón, Erika Ruiz-García, Abelardo Meneses García, Olga N Hernández De La Cruz, Horacio Astudillo-De La Vega, David Isla-Ortiz, Laurence A Marchat, Yarely M Salinas-Vera, Ángeles Carlos-Reyes, Sullivan López-González, Rosalio Ramos-Payan, César López-Camarillo
Altered expression of microRNAs contributes to the heterogeneous biological behavior of human malignancies and it may correlate with the clinical pathological features of patients. The let-7 microRNA family is frequently downregulated in human cancers and its aberrant expression may be a useful marker for prediction of the clinical response to therapy in patients. In the present study, we analyzed the expression of three members of the let-7 family (let-7a-3p, let-7d-3p and let-7f), which remains largely uncharacterized in ovarian cancer tissues...
April 12, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29651284/transcriptomic-and-ectoine-analysis-of-halotolerant-nocardiopsis-gilva-yim-90087-t-under-salt-stress
#19
Jian Han, Quan-Xiu Gao, Yong-Guang Zhang, Li Li, Osama A A Mohamad, Manik Prabhu Narsing Rao, Min Xiao, Wael N Hozzein, Dalal H M Alkhalifah, Yong Tao, Wen-Jun Li
The genus Nocardiopsis is an unique actinobacterial group that widely distributed in hypersaline environments. In this study, we investigated the growth conditions, transcriptome analysis, production and accumulation of ectoine by Nocardiopsis gilva YIM 90087T under salt stress. The colony color of N. gilva YIM 90087T changed from yellow to white under salt stress conditions. Accumulation of ectoine and hydroxyectoine in cells was an efficient way to regulate osmotic pressure. The ectoine synthesis was studied by transferring the related genes ( ectA, ectB , and ectC ) to Escherichia coli ...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29643473/revisiting-the-role-of-abc-transporters-in-multidrug-resistant-cancer
#20
REVIEW
Robert W Robey, Kristen M Pluchino, Matthew D Hall, Antonio T Fojo, Susan E Bates, Michael M Gottesman
Most patients who die of cancer have disseminated disease that has become resistant to multiple therapeutic modalities. Ample evidence suggests that the expression of ATP-binding cassette (ABC) transporters, especially the multidrug resistance protein 1 (MDR1, also known as P-glycoprotein or P-gp), which is encoded by ABC subfamily B member 1 (ABCB1), can confer resistance to cytotoxic and targeted chemotherapy. However, the development of MDR1 as a therapeutic target has been unsuccessful. At the time of its discovery, appropriate tools for the characterization and clinical development of MDR1 as a therapeutic target were lacking...
April 11, 2018: Nature Reviews. Cancer
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