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https://www.readbyqxmd.com/read/28448035/bioluminescence-imaging-of-neuroinflammation-in-transgenic-mice-after-peripheral-inoculation-of-alpha-synuclein-fibrils
#1
Sara Breid, Maria E Bernis, Julius B Tachu, Maria C Garza, Holger Wille, Gültekin Tamgüney
To study the prion-like behavior of misfolded alpha-synuclein, mouse models are needed that allow fast and simple transmission of alpha-synuclein prionoids, which cause neuropathology within the central nervous system (CNS). Here we describe that intraglossal or intraperitoneal injection of alpha-synuclein fibrils into bigenic Tg(M83(+/-):Gfap-luc(+/-)) mice, which overexpress human alpha-synuclein with the A53T mutation from the prion protein promoter and firefly luciferase from the promoter for glial fibrillary acidic protein (Gfap), is sufficient to induce neuropathologic disease...
April 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28442942/comparison-in-the-quality-of-distractors-in-three-and-four-options-type-of-multiple-choice-questions
#2
Nourelhouda A A Rahma, Mahdi M A Shamad, Muawia E A Idris, Omer Abdelgadir Elfaki, Walyedldin E M Elfakey, Karimeldin M A Salih
INTRODUCTION: The number of distractors needed for high quality multiple choice questions (MCQs) will be determined by many factors. These include firstly whether English language is their mother tongue or a foreign language; secondly whether the instructors who construct the questions are experts or not; thirdly the time spent on constructing the options is also an important factor. It has been observed by Tarrant et al that more time is often spent on constructing questions than on tailoring sound, reliable, and valid distractors...
2017: Advances in Medical Education and Practice
https://www.readbyqxmd.com/read/28440544/-age-related-effect-of-cell-death-on-fiber-morphology-and-number-in-tongue-muscle
#3
Heidi Kletzien, Allison J Hare, Glen Leverson, Nadine P Connor
INTRODUCTION: Multiple pathways may exist for age-related tongue muscle degeneration. Cell death is one mechanism contributing to muscle atrophy and decreased function. We hypothesized with aging, apoptosis and apoptotic regulators would be increased, and muscle fiber size and number would be reduced in extrinsic tongue muscles. METHODS: Cell death indices, expression of caspase-3 and Bcl-2, and measures of muscle morphology and number were determined in extrinsic tongue muscles of young and old rats...
April 25, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/28439442/a-prospective-randomised-clinical-trial-for-the-comparison-of-two-techniques-for-the-insertion-of-proseal-laryngeal-mask-airway-in-adults-index-finger-insertion-technique-versus-90%C3%A2-rotation-technique
#4
Pavan V Dhulkhed, Sunil V Khyadi, Parbati B Jamale, Vithal K Dhulkhed
OBJECTIVE: The 90° rotation technique for inserting the Proseal laryngeal mask airway (PLMA) is reported to be better than the standard index finger insertion technique to improve the insertion success rate. The objective of this study was to evaluate and compare the ease of insertion through the 90° rotation and standard insertion techniques in terms of number of attempts, duration of insertion and occurrence of complications. METHODS: One hundred and twenty adult patients were allocated to either a standard technique group or rotation technique group with 60 patients in each...
April 2017: Turkish Journal of Anaesthesiology and Reanimation
https://www.readbyqxmd.com/read/28438836/a-wnt-notch-pax7-signaling-network-supports-tissue-integrity-in-tongue-development
#5
Xiao-Jing Zhu, Xueyan Yuan, Min Wang, Yukun Fang, Yudong Liu, Xiaoyun Zhang, Xueqin Yang, Yan Li, Jianying Li, Feixue Li, Zhong-Min Dai, Mengsheng Qiu, Ze Zhang, Zunyi Zhang
The tongue is one of the major structures involved in human food intake and speech. Tongue malformations such as aglossia, microglossia, and ankyloglossia are congenital birth defects, greatly affecting individuals' quality of life. However, the molecular basis of the tissue-tissue interactions that ensure tissue morphogenesis to form a functional tongue remains largely unknown. Here we show that ShhCre-mediated epithelial deletion of Wntless (Wls), the key regulator for intracellular Wnt trafficking, leads to lingual hypoplasia in mice...
April 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28435450/exome-sequencing-of-oral-squamous-cell-carcinoma-reveals-molecular-subgroups-and-novel-therapeutic-opportunities
#6
Shih-Chi Su, Chiao-Wen Lin, Yu-Fan Liu, Wen-Lang Fan, Mu-Kuan Chen, Chun-Ping Yu, Wei-En Yang, Chun-Wen Su, Chun-Yi Chuang, Wen-Hsiung Li, Wen-Hung Chung, Shun-Fa Yang
Oral squamous cell carcinoma (OSCC), an epithelial malignancy affecting a variety of subsites in the oral cavity, is prevalent in Asia. The survival rate of OSCC patients has not improved over the past decades due to its heterogeneous etiology, genetic aberrations, and treatment outcomes. Improvement in therapeutic strategies and tailored treatment options is an unmet need. To unveil the mutational spectrum, whole-exome sequencing of 120 OSCC from male individuals in Taiwan was conducted. Analyzing the contributions of the five mutational signatures extracted from the dataset of somatic variations identified four groups of tumors that were significantly associated with demographic and clinical features...
2017: Theranostics
https://www.readbyqxmd.com/read/28434900/breast-cancer-in-a-pectoralis-major-myocutaneous-flap-used-for-the-reconstruction-of-tongue-cancer-a-case-report
#7
Eiji Hirai, Shunji Sarukaw, Kozo Yamamoto, Masahiro Okamoto
Breast cancers are the most common cancers in women. However, breast cancer occurring in a pectoralis major myocutaneous flap is extremely rare. This article describes a case of breast cancer occurring in such a flap used for reconstruction of the tongue in a 72-year-old woman. Follow-up computed tomogram depicted a slowly growing mass in the flap. Thirty-nine months postoperatively, a fine-needle aspiration biopsy specimen taken from the lesion suggested glandular carcinoma. The patient was diagnosed with breast cancer in the neck area of the flap and tumor excision was performed...
March 29, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28434085/exploring-the-oral-microflora-of-preschool-children
#8
Wen Ren, Qun Zhang, Xuenan Liu, Shuguo Zheng, Lili Ma, Feng Chen, Tao Xu, Baohua Xu
The oral cavity is one of the most important and complicated habitats in our body and supports diverse microbial communities. In this study, we aimed to determine the bacterial diversity and composition of various oral micro-niches. Samples were collected from supragingival plaque, saliva, and tongue coating from 10 preschool children (30 samples total). 16S rRNA gene pyrosequencing dataset generated 314,639 clean reads with an average of 10,488 ± 2,787 reads per sample. The phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Fusobacteria were predominant, accounting for more than 90% of the total sequences...
April 22, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28419671/pediatric-oral-injuries-presented-to-a-japanese-pediatric-emergency-room
#9
Nobuaki Inoue
BACKGROUND: Oral injuries are common among children, but the study comparing oral injuries between oral injuries with foreign objects in children's mouth and without objects is limited. The aim of this study is to compare injury patterns and their effects to children who presented to a Japanese pediatric emergency room (ER) with and without foreign objects in their mouth. METHODS: Children who were brought to the emergency room in Tokyo Metropolitan Medical Center for oral injuries from January 1(st) , 2011 to December 31(st) , 2012, were included...
April 17, 2017: Pediatrics International: Official Journal of the Japan Pediatric Society
https://www.readbyqxmd.com/read/28417103/effect-of-age-and-severity-of-facial-palsy-on-taste-thresholds-in-bell-s-palsy-patients
#10
Jung Min Park, Myung Gu Kim, Junyang Jung, Sung Su Kim, A Ra Jung, Sang Hoon Kim, Seung Geun Yeo
BACKGROUND AND OBJECTIVES: To investigate whether taste thresholds, as determined by electrogustometry (EGM) and chemical taste tests, differ by age and the severity of facial palsy in patients with Bell's palsy. SUBJECTS AND METHODS: This study included 29 patients diagnosed with Bell's palsy between January 2014 and May 2015 in our hospital. Patients were assorted into age groups and by severity of facial palsy, as determined by House-Brackmann Scale, and their taste thresholds were assessed by EGM and chemical taste tests...
April 2017: Journal of Audiology & Otology
https://www.readbyqxmd.com/read/28413553/excision-of-hypoglossal-neurinoma-by-condyle-sparing-suboccipital-keyhole-approach
#11
Harjinder Bhatoe
Hypoglossal neurinomas are rare tumors that present with gradually worsening unilateral wasting of the tongue. The tumors often attain large size prior to detection and often extend into the hypoglossal canal. Large tumors can distort the brainstem and cerebellum. Preoperative diagnosis can often be made by magnetic resonance imaging (MRI) with contrast enhancement. Traditionally, surgery for these tumors has entailed large transcondylar approaches, often necessitating occipitocervical stabilization. We managed one 23-year-old female patient with a large hypoglossal neurinoma...
January 2017: Asian Journal of Neurosurgery
https://www.readbyqxmd.com/read/28412967/factors-associated-with-lingual-tonsil-hypertrophy-in-canadian-adults
#12
Matthew S Harris, Brian W Rotenberg, Kathryn Roth, Leigh J Sowerby
BACKGROUND: Hypertrophy of the lingual tonsil tissue in the adult patient is thought to contribute to the pathophysiology of obstructive sleep apnea. The underlying etiology of lingual tonsil hypertrophy (LTH) in the adult patient is unclear and likely multifactorial. Previous studies have suggested that the lingual tonsils may undergo compensatory hyperplasia post-tonsillectomy in children, although it is unknown if this occurs or persists into adulthood. The purpose of this study was to determine what factors are associated with LTH in a population of Canadian adults...
April 17, 2017: Journal of Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/28412801/-preliminary-report-on-the-application-of-femtosecond-laser-assisted-anterior-capsulotomy-in-intumescent-white-cataract-surgery
#13
T T Peng, Y Wang, X Y Bao
Objective: To assess the effect of femtosecond laser-assisted anterior capsulotomy in intumescent white cataract surgery. Methods: Retrospective case series study. One hundred and fifty cataract patients (150 eyes) from June 2014 to June 2015 in Wuhan Aier ophthalmology hospital were enrolled in this study. They were divided into three groups: 50 intumescent white cataract patients (50 eyes) in the study group were performed femtosecond laser-assisted capsulotomy (LenSx system) operation selectively. Fifty intumescent white cataract patients (50 eyes) underwent normal manual continuous circular capsulorhexis as the control group 1...
April 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28403413/can-tongue-thickness-measured-by-ultrasonography-predict-difficult-tracheal-intubation
#14
W Yao, Bin Wang
Background.: Increased tongue thickness is likely to be associated with difficult airways. However, no methods to evaluate tongue thickness were available. Currently, tongue thickness can be measured by ultrasonography. The present study investigated the predictive value of tongue thickness to predict difficult tracheal intubation. Methods.: Adult patients undergoing tracheal intubation and general anaesthesia were enrolled in the study. Tongue thickness was assessed using submental ultrasonography in the median sagittal plane before anaesthesia...
April 1, 2017: British Journal of Anaesthesia
https://www.readbyqxmd.com/read/28403280/electropalatography-and-its-correlation-to-tongue-movement-ultrasonography-in-speech-analysis
#15
Luciana da Silva Barberena, Caroline Rodrigues Portalete, Simone Nicolini de Simoni, Ana Cristina Melo Prates, Márcia Keske-Soares, Renata Mancopes
Purpose: To analyze the use of electropalatography and ultrasonography regarding speech therapy through literature narrative review. Research strategies: A literature review was conducted at PubMed and Scielo databases, using descriptors as electropalatography, electropalatography AND evaluation, electropalatography AND therapy, electropalatography AND ultrasonography, electropalatography AND speech. Selection criteria: The research criteria selected in the database were: studies in the past five years and studies in humans...
April 10, 2017: CoDAS
https://www.readbyqxmd.com/read/28403069/using-a-pedicle-pectoralis-major-musculocutaneous-flap-in-head-and-neck-reconstruction-after-modified-radical-mastectomy-a-case-report
#16
Jen-Wu Huang, Nai-Yuan Wu, Yi-Ying Lin
RATIONALE: A pedicle pectoralis major musculocutaneous (PMMC) flap is one of the strategies for head and neck reconstruction. Seldom studies reported the case in which the skin area of previous modified radical mastectomy (MRM) had been used as a PMMC flap in head and neck reconstruction. PATIENT CONCERNS: An 84-year-old female who had suffered from left breast cancer and undergone a left modified radical mastectomy (MRM) more than 20 years earlier. DIAGNOSES: She had squamous cell carcinoma of the tongue and had undergone partial glossection and left modified radial neck dissection...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28396208/efficacy-of-a-high-potency-o1-manisa-foot-and-mouth-disease-vaccine-in-cattle-against-heterologous-challenge-with-a-field-virus-from-the-o-me-sa-ind-2001-lineage-collected-in-north-africa
#17
Emma Fishbourne, Anna B Ludi, Ginette Wilsden, Pip Hamblin, Bob Statham, Abdelghani Bin-Tarif, Emiliana Brocchi, Santina Grazioli, Aldo Dekker, Phaedra Eblé, Donald P King
Outbreaks of foot-and-mouth disease (FMD) in North Africa (2013) and the Gulf States (2013) of the Middle East have been caused by a FMD viral lineage (O/ME-SA/Ind-2001) that was before 2013 restricted to the Indian Sub-continent. This study was undertaken to assess the in vivo efficacy of a FMD virus emergency vaccine type O1 Manisa against heterologous challenge with a representative field virus (O/ALG/3/2014) from this emerging lineage. This widely available vaccine was selected since in vitro vaccine-matching results gave inconclusive results as to whether or not it would be protective...
May 9, 2017: Vaccine
https://www.readbyqxmd.com/read/28390748/effectiveness-of-radiotherapy-ozone-on-tumoral-tissue-and-survival-in-tongue-cancer-rat-model
#18
Remzi Dogan, Aysenur Meric Hafız, Huriye Senay Kiziltan, Alper Yenigun, Nur Buyukpinarbaslili, Ali Hikmet Eris, Orhan Ozturan
OBJECTIVE: The objective of this study is to examine therapeutic effect of the combination of radiotherapy and ozone which features increasing of the destruction of cancer cells by increasing oxygen level of the body on advanced tongue cancer induced in rats. METHODS: A total of 36 female rats were included in this study as 4 groups. Group 1 (Cancer, n=8): 4NQO. Group 2 (Cancer+Radiotherapy, n=10): 4NQO+Radiotherapy. Group 3 (Cancer+Ozone+Radiotherapy, n=10): 4NQO+Ozone+Radiotherapy...
April 5, 2017: Auris, Nasus, Larynx
https://www.readbyqxmd.com/read/28390130/oral-diseases-a-14-year-experience-of-a-chilean-institution-with-a-systematic-review-from-eight-countries
#19
C Rivera, C Jones-Herrera, P Vargas, B Venegas, D Droguett
BACKGROUND: Retrospective studies to assess the distribution of oral diseases (ODs) are helpful in estimating the prevalence of oral diagnoses in the population, and thus help in preventive and curative services. Prevalence and frequency data for ODs are available from many countries, but information from Chile is scarce. MATERIAL AND METHODS: This study investigated the frequency of OD in a Chilean population. For this, we included all patients treated at the University of Talca (UTALCA, Chile) between 2001 and 2014...
April 8, 2017: Medicina Oral, Patología Oral y Cirugía Bucal
https://www.readbyqxmd.com/read/28370229/more-than-just-the-helix-a-series-of-free-flaps-from-the-ear
#20
Patrik Lassus, Annastiina Husso, Jyrki Vuola, Andrew J Lindford
AIM: We herein present our experience using free flaps harvested from the ear region in facial, nasal and intraoral reconstruction. PATIENTS AND METHODS: Between 2011 and 2016, 19 patients underwent reconstruction using 20 free flaps from the ear region based on the superficial temporal vessels. There were 10 males and 9 females with a mean age of 57 years. Defect aetiology consisted of post-tumour ablation (n = 15), trauma (n = 2) and burn scar (n = 2)...
April 1, 2017: Microsurgery
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