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https://www.readbyqxmd.com/read/29051092/a-study-on-the-targeted-surveillance-of-nosocomial-infection-in-intensive-care-units-of-177-hospitals-in-jiangsu-province-of-china
#1
Yang Li, Xiao-Li Cao, Hai Ge, Yi-Hong Jiang, Hong Zhou, Wei Zheng
BACKGROUND: Nosocomial infections (NIs) impact care and costs in hospitals across the globe. There are minimal data on targeted surveillance of NI in intensive care units (ICUs), and data specific to the risk factors for NI are especially limited. METHODS: One hundred seventy-seven secondary and tertiary hospitals performed NI targeted surveillance in their ICUs. The data were collected and summarized by Minke software, then fed back once per quarter. RESULTS: The incidence of NI appeared to decrease, and the incidence of NI per 1000 patient-days and adjusted incidence were 25...
October 16, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29030089/knockdown-of-hif-1%C3%AE-inhibits-the-proliferation-and-migration-of-outer-root-sheath-cells-exposed-to-hypoxia-in-vitro-an-involvement-of-shh-pathway
#2
Haihua Zhang, Weixiao Nan, Xingchao Song, Shiyong Wang, Huazhe Si, Guangyu Li
AIMS: Outer root sheath (ORS) is a highly proliferative component of a hair follicle. This study is performed to investigate whether hypoxia-induced elevation of hypoxia-inducible factor (HIF)-1α, a transcriptional activator, contributes to the outgrowth of ORS cells in vitro. MAIN METHODS: Hair follicles with intact ORS collected from 4-month old male American minks were cultured in normoxic or hypoxic condition (3% oxygen) for 7days. Primary ORS cells isolated from the mink hair follicles were exposed to hypoxia for 12, 24 or 48h, and their proliferation was analyzed with immunofluorescence assay using anti-proliferating cell nuclear antigen (PCNA) antibody...
October 10, 2017: Life Sciences
https://www.readbyqxmd.com/read/28984379/use-of-pcb-and-teq-concentrations-in-scat-from-mink-neovison-vison-fed-fish-from-the-upper-hudson-river-to-predict-dietary-and-hepatic-concentrations-and-health-effects
#3
Steven J Bursian, John Kern, Richard E Remington, Jane E Link
To evaluate health effects associated with consumption of PCB-contaminated fish from the upper Hudson River, farm-raised mink were fed diets containing fish collected from the river. Endpoints assessed included adult reproductive performance, offspring growth and mortality, and organ mass and pathology of adults and their offspring. Scat samples were collected from adult males at the time of necropsy and from adult females just prior to whelping. Scat samples were analyzed for polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs)...
October 6, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28963476/comparative-transcriptome-analysis-of-mink-neovison-vison-skin-reveals-the-key-genes-involved-in-the-melanogenesis-of-black-and-white-coat-colour
#4
Xingchao Song, Chao Xu, Zongyue Liu, Zhigang Yue, Linling Liu, Tongao Yang, Bo Cong, Fuhe Yang
Farmed mink (Neovison vison) is one of the most important fur-bearing species worldwide, and coat colour is a crucial qualitative characteristic that contributes to the economic value of the fur. To identify additional genes that may play important roles in coat colour regulation, Illumina/Solexa high-throughput sequencing technology was used to catalogue the global gene expression profiles in mink skin with two different coat colours (black and white). RNA-seq analysis indicated that a total of 12,557 genes were differentially expressed in black versus white minks, with 3,530 genes up-regulated and 9,027 genes down-regulated in black minks...
September 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28954472/comparative-molecular-analysis-of-strains-of-the-aleutian-disease-virus-isolated-from-farmed-and-wild-mink
#5
Andrzej Jakubczak, Marek Kowalczyk, Krzysztof Kostro, Grazyna Jezewska-Witkowska
INTRODUCTION AND OBJECTIVE: Aleutian Disease is a significant biological factor causing substantial losses in mink farming. The virus inducing the disease also infects wild populations which may constitute an asymptomatic reservoir. To compare genetic variants of the AMD virus occurring in wild and farmed mink populations, an analysis was performed on a fragment of the VP2 protein sequence of the virus infecting both populations, taken from different living environments. MATERIAL AND METHODS: Genetic material was isolated from 11 farmed animals in which anti-AMDV antibodies had been detected and from 20 wild animals...
September 21, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28952752/field-line-localized-destabilization-of-ballooning-modes-in-three-dimensional-tokamaks
#6
M Willensdorfer, T B Cote, C C Hegna, W Suttrop, H Zohm, M Dunne, E Strumberger, G Birkenmeier, S S Denk, F Mink, B Vanovac, L C Luhmann
Field-line localized ballooning modes have been observed at the edge of high confinement mode plasmas in ASDEX Upgrade with rotating 3D perturbations induced by an externally applied n=2 error field and during a moderate level of edge localized mode mitigation. The observed ballooning modes are localized to the field lines which experience one of the two zero crossings of the radial flux surface displacement during one rotation period. The localization of the ballooning modes agrees very well with the localization of the largest growth rates from infinite-n ideal ballooning stability calculations using a realistic 3D ideal magnetohydrodynamic equilibrium...
August 25, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28952075/persistent-changes-in-circulating-white-blood-cell-populations-after-splenectomy
#7
Minke A E Rab, Aafke Meerveld-Eggink, Heleen van Velzen-Blad, Douwe van Loon, Ger T Rijkers, Okke de Weerdt
The effect of splenectomy on the incidence of infections and thromboembolisms has been investigated thoroughly. Nevertheless, the long-term effects of splenectomy on immunological profile and circulating blood counts have not been described before. To study such long-term effects, we analysed several parameters in splenectomised trauma patients and compared the results of this group ("otherwise healthy patients") to patients with a specific underlying disease. We measured platelet count, leukocytes and differential, lymphocyte subsets, serum levels of immunoglobulins, and complement pathways in 113 patients...
September 26, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/28951203/high-resolution-phylogeny-providing-insights-towards-the-epidemiology-zoonotic-aspects-and-taxonomy-of-sapoviruses
#8
A F Barry, R Durães-Carvalho, E F Oliveira-Filho, A A Alfieri, W H M Van der Poel
The evolution, epidemiology and zoonotic aspects of Sapoviruses (SaV) are still not well explored. In this study, we applied high-resolution phylogeny to investigate the epidemiological and zoonotic origins as well as taxonomic classification of animal and human SaV. Bayesian framework analyses showed an increase in porcine SaV (PoSaV) population dynamics and genetic diversity between 1975 and 1982, resulting in a SaV gene flow and generation of new strains among porcine and human populations. Our results also show the contribution of different animal populations involved in SaV epidemiology and highlight zoonotic aspects, as exemplified by the crucial role that swine, dogs, mink and humans play in SaV spread...
September 23, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28941157/hepatitis-e-virus-detected-from-chinese-laboratory-ferrets-and-farmed-mink
#9
Lin Wang, W Gong, H Fu, M Li, Y Zhang, Z Luo, Q Xu, L Wang
A novel genotype of hepatitis E virus (HEV) has been discovered in ferrets and mink worldwide. However, the situation in China is unknown. Diverse HEV RNA belonging to HEV genotype C2 was detected in this study from 17.5% (18/103) of the laboratory ferrets and 1.9% (2/107) of farmed mink in China. Phylogenetic analysis showed that the ferret HEV strains clustered into three groups with known strains detected from the United States and the Netherlands. Attempted transmission of ferret HEV to two rabbits was not successful...
September 22, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28923508/generation-of-an-infectious-clone-of-amdv-and-identification-of-capsid-residues-essential-for-infectivity-in-cell-culture
#10
Ji Xi, Yanlong Zhang, Jigui Wang, Yongle Yu, Xiaomei Zhang, Zhaoda Li, Shangjin Cui, Weiquan Liu
Pathogenic strains of Aleutian mink disease virus (AMDV) such as Utah-1 do not replicate in cell culture (e.g., Crandell Rees feline kidney cells) while the in vitro-adapted AMDV strain ADV-Gorham (ADV-G) is not pathogenic. Here, we constructed a full-length infectious clone (pADV-G). Alignment of the VP2 gene of ADV-G with that of other AMDV strains revealed many amino acid (a.a.) residues conserved among pathogenic isolates that differed in ADV-G. Four virulence-associated, conserved residues of pADV-G VP2 were studied by site-directed mutagenesis (H92A, Q94S, Y115F, and I116L)...
September 18, 2017: Virus Research
https://www.readbyqxmd.com/read/28917308/prevalence-of-pentatrichomonas-hominis-infections-in-six-farmed-wildlife-species-in-jilin-china
#11
Xianhe Li, Jianhua Li, Xichen Zhang, Zhengtao Yang, Ju Yang, Pengtao Gong
Pentatrichomonas hominis is an anaerobic flagellated protozoan that primarily parasitizes the gastrointestinal tract and is a conditional pathogen. It has an extensive host range and is well known as a potential causative agent of zoonotic disease. The objective of this study was to provide the first findings of the prevalence of P. hominis in six farmed wildlife species, sika deer (S.D.), Rex rabbits (R.R.), blue foxes (B.F.), silver foxes (S.F.), raccoon dogs (R.D.) and minks (M.), that are commercially important in Jilin Province, China...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28903765/antimicrobial-resistance-among-pathogenic-bacteria-from-mink-neovison-vison-in-denmark
#12
Nanett Kvist Nikolaisen, Desireé Corvera Kløve Lassen, Mariann Chriél, Gitte Larsen, Vibeke Frøkjær Jensen, Karl Pedersen
BACKGROUND: For proper treatment of bacterial infections in mink, knowledge of the causative agents and their antimicrobial susceptibility patterns is crucial. The used antimicrobials are in general not registered for mink, i.e. most usage is "off-label". In this study, we report the patterns of antimicrobial resistance among pathogenic bacteria isolated from Danish mink during the period 2014-2016. The aim of this investigation was to provide data on antimicrobial resistance and consumption, to serve as background knowledge for new veterinary guidelines for prudent and optimal antimicrobial usage in mink...
September 13, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28888627/genetic-and-pathogenic-characterization-of-a-novel-reassortant-mammalian-orthoreovirus-3-mrv3-from-a-diarrheic-piglet-and-seroepidemiological-survey-of-mrv3-in-diarrheic-pigs-from-east-china
#13
Pan Qin, Huan Li, Jing-Wei Wang, Bin Wang, Rong-Hui Xie, Hui Xu, Ling-Yan Zhao, Long Li, Yongfei Pan, Yanhua Song, Yao-Wei Huang
Mammalian orthoreoviruses (MRVs), which cause gastrointestinal and respiratory illness, have been isolated from a wide variety of mammalian species including bats, minks, pigs and humans. Here we report the isolation and genetic and pathogenic characterization of a novel MRV type 3 (MRV3), named MRV-ZJ2013, from the diarrheic feces of piglets in Zhejiang province, China. Genomic and phylogenetic analysis shows that MRV-ZJ2013 may have originated from reassortments among mink, bat, and pig MRVs, suggesting the hypothesis that interspecies transmission has occurred in pig herds...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28886754/on-farm-biosecurity-practices-and-causes-of-preweaning-mortality-in-canadian-commercial-mink-kits
#14
Nicole Compo, David L Pearl, Brian Tapscott, Amanda Storer, Jutta Hammermueller, Marina Brash, Patricia V Turner
BACKGROUND: Mink are an important animal commodity group in Canada and excessive kit mortality represents a significant loss to production. National biosecurity standards have been developed for Canadian mink farms, but it is unclear how well these standards have been implemented as there are no studies correlating management practices of mink producers with causes of death in mink kits. To that end, we surveyed Ontario mink producers on their biosecurity and management practices and conducted almost 5660 post mortem examinations on found-dead, preweaned kits to characterize mink farm biosecurity practices and causes of death in preweaned kits...
September 8, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28873943/novel-sequence-types-of-extended-spectrum-and-acquired-ampc-beta-lactamase-producing-escherichia-coli-and-escherichia-clade-v-isolated-from-wild-mammals
#15
Carla Andrea Alonso, Leticia Alcalá, Carmen Simón, Carmen Torres
The closer contact with wildlife due to the growing human population and the destruction of natural habitats emphasizes the need of gaining insight into the role of animals as source of antimicrobial resistance. Here, we aim at characterizing the antimicrobial resistance genes and phylogenetic distribution of commensal Escherichia coli from 62 wild mammals. Isolates exhibiting resistance to ≥1 antibiotic were detected in 25.8% of the animals and 6.4% carried an extended-spectrum beta-lactamase (ESBL)/AmpC-producing E...
August 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28871070/pearls-oy-sters-frontal-lobe-epilepsy-presenting-as-complex-stereotyped-movements
#16
Jennifer Vermilion, Jonathan W Mink
No abstract text is available yet for this article.
September 5, 2017: Neurology
https://www.readbyqxmd.com/read/28867359/novel-heterozygous-notch3-pathogenic-variant-found-in-two-chinese-patients-with-cadasil
#17
Shufeng Li, Yifan Chen, Haitao Shan, Fang Ma, Minke Shi, Jun Xue
NOTCH3 mutations have been described to cause cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Here, we report 2 CADASIL patients from a Chinese family. Whole genome sequencing was performed on the two CADASIL patients. The novel variant c.128G>C in exon 2 of NOTCH3 was identified and confirmed through PCR-Sanger sequencing (Human Genome Variation Society nomenclature: HGVS: NOTCH3 c.128G>C; p.Cys43Ser). The heterozygous NOTCH3 variant cause a cysteine to serine substitution at codon 43...
August 31, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28863818/depletion-of-membrane-cholesterol-suppresses-drosophila-transient-receptor-potential-like-trpl-channel-activity
#18
Maximilian Peters, Ben Katz, Shaya Lev, Rachel Zaguri, Rita Gutorov, Baruch Minke
Cholesterol is an essential compound of higher eukaryotic cell membranes and a known modulator of ion channel activity. Changes in phospholipids and cholesterol composition of cell membranes are known to alter the activity of ion channels. However, there is little knowledge on the effects of cholesterol on transient receptor potential (TRP) channels. In this study, we explore the effects of cholesterol depletion on the Drosophila photoreceptor channel TRP-like (TRPL), when expressed in tissue culture cells...
2017: Current Topics in Membranes
https://www.readbyqxmd.com/read/28854681/short-term-effect-of-oral-amoxicillin-treatment-on-the-gut-microbial-community-composition-in-farm-mink-neovison-vison
#19
Laura Meier Marker, Anne Sofie Hammer, Lars Andresen, Pernille Isaack, Tove Clausen, Kevin Byskov, Oliver Lykke Honoré, Søren Krogh Jensen, Martin Iain Bahl
It is well documented that antibiotics have pronounced modulatory effects on the intestinal bacterial community of both humans and animals, with potential health consequences. The gut microbiota of mink has however attracted little attention due to low bacterial load and fast gastrointestinal transit time, questioning its relevance. In this study, we hypothesise that oral amoxicillin treatment affects the gut microbiota in mink. This was investigated in a controlled trial including 24 animals of which 12 were treated with amoxicillin for 7 days...
July 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28854252/molecular-markers-in-keratins-from-mysticeti-whales-for-species-identification-of-baleen-in-museum-and-archaeological-collections
#20
Caroline Solazzo, William Fitzhugh, Susan Kaplan, Charles Potter, Jolon M Dyer
Baleen has been harvested by indigenous people for thousands of years, as well as collected by whalers as an additional product of commercial whaling in modern times. Baleen refers to the food-filtering system of Mysticeti whales; a full baleen rack consists of dozens of plates of a tough and flexible keratinous material that terminate in bristles. Due to its properties, baleen was a valuable raw material used in a wide range of artefacts, from implements to clothing. Baleen is not widely used today, however, analyses of this biomolecular tissue have the potential to contribute to conservation efforts, studies of genetic diversity and a better understanding of the exploitation and use of Mysticeti whales in past and recent times...
2017: PloS One
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