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https://www.readbyqxmd.com/read/28578412/hsp70-facilitates-trans-membrane-transport-of-bacterial-adp-ribosylating-toxins-into-the-cytosol-of-mammalian-cells
#1
Katharina Ernst, Johannes Schmid, Matthias Beck, Marlen Hägele, Meike Hohwieler, Patricia Hauff, Anna Katharina Ückert, Anna Anastasia, Michael Fauler, Thomas Jank, Klaus Aktories, Michel R Popoff, Cordelia Schiene-Fischer, Alexander Kleger, Martin Müller, Manfred Frick, Holger Barth
Binary enterotoxins Clostridium (C.) botulinum C2 toxin, C. perfringens iota toxin and C. difficile toxin CDT are composed of a transport (B) and a separate non-linked enzyme (A) component. Their B-components mediate endocytic uptake into mammalian cells and subsequently transport of the A-components from acidic endosomes into the cytosol, where the latter ADP-ribosylate G-actin resulting in cell rounding and cell death causing clinical symptoms. Protein folding enzymes, including Hsp90 and peptidyl-prolyl cis/trans isomerases facilitate transport of the A-components across endosomal membranes...
June 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28574444/validation-of-the-performance-of-international-ovarian-tumor-analysis-iota-methods-in-the-diagnosis-of-early-stage-ovarian-cancer-in-a-non-screening-population
#2
Wouter Froyman, Laure Wynants, Chiara Landolfo, Tom Bourne, Lil Valentin, Antonia Testa, Povilas Sladkevicius, Dorella Franchi, Daniela Fischerova, Luca Savelli, Ben Van Calster, Dirk Timmerman
BACKGROUND: The aim of this study was to assess and compare the performance of different ultrasound-based International Ovarian Tumor Analysis (IOTA) strategies and subjective assessment for the diagnosis of early stage ovarian malignancy. METHODS: This is a secondary analysis of a prospective multicenter cross-sectional diagnostic accuracy study that included 1653 patients recruited at 18 centers from 2009 to 2012. All patients underwent standardized transvaginal ultrasonography by experienced ultrasound investigators...
June 2, 2017: Diagnostics
https://www.readbyqxmd.com/read/28574228/apkc-%C3%AE-p-sp1-snail-signaling-induces-epithelial-mesenchymal-transition-and-immunosuppression-in-cholangiocarcinoma
#3
Yawei Qian, Wei Yao, Tao Yang, Yan Yang, Yan Liu, Qi Shen, Jian Zhang, Weipeng Qi, Jianming Wang
Cholangiocarcinoma (CCA) is a highly malignant bile duct cancer that tends to invade and metastasize early. The epithelial-mesenchymal transition (EMT) has been implicated in cancer cell invasion and metastasis, as well as in cancer cell evasion of host immunity. In this study, we investigated the interaction between atypical protein kinase C-iota (aPKC-ι) and Snail in the regulation of EMT and its relationship to CCA immunosuppression. Our results demonstrated that aPKC-ι, Snail, and infiltrated immunosuppressive cells were significantly up-regulated in CCA tumor tissues and were linked to poor prognosis...
June 2, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28528740/angubindin-1-a-novel-paracellular-absorption-enhancer-acting-at-the-tricellular-tight-junction
#4
Susanne M Krug, Tomohiro Hayaishi, Daisuke Iguchi, Akihiro Watari, Azusa Takahashi, Michael Fromm, Masahiro Nagahama, Hiroyuki Takeda, Yoshiaki Okada, Tatsuya Sawasaki, Takefumi Doi, Kiyohito Yagi, Masuo Kondoh
A limiting barrier for mucosal absorption of drugs is the tight junction (TJ). TJs exist between two adjacent cells (bicellular TJ, bTJ) and at the sites where three cells meet (tricellular TJ, tTJ). We present a novel approach which employs a physiologically regulated pathway for the passage of large molecules through the tTJ. Main barrier-relevant tTJ proteins are tricellulin and angulin-1 to -3. We developed an angulin binder from Clostridium perfringens iota-toxin (Ib) whose receptor is angulin-1. An Ib fragment corresponding to amino acids 421-664 (Ib421-664) of iota-toxin proved to bind in cells expressing angulin-1 and -3, but not angulin-2...
May 18, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28512173/clinical-utility-of-risk-models-to-refer-patients-with-adnexal-masses-to-specialized-oncology-care-multicenter-external-validation-using-decision-curve-analysis
#5
Laure Wynants, Dirk Timmerman, Jan Verbakel, Antonia Testa, Luca Savelli, Daniela Fischerová, Dorella Franchi, Caroline D Van Holsbeke, Elisabeth Epstein, Wouter Froyman, Stefano Guerriero, Alberto Rossi, Robert Fruscio, Francesco Leone, Tom Bourne, Lil Valentin, Ben Van Calster
To evaluate the utility of pre-operative diagnostic models for ovarian cancer based on ultrasound and/or biomarkers for referring patients to specialized oncology care. The investigated models were RMI, ROMA, and three models from the International Ovarian Tumor Analysis (IOTA) group (LR2, ADNEX, and the Simple Rules risk score, SRRisks). <p>DESIGN: A secondary analysis of prospectively collected data from two cross-sectional cohort studies performed to externally validate diagnostic models.</p> <p>PARTICIPANTS: A total of 2763 patients (2403 in dataset 1 and 360 in dataset 2) from 18 centers (11 oncology centers and 7 non-oncology hospitals) in 6 countries...
May 16, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28464022/a-central-region-in-the-minor-capsid-protein-of-papillomaviruses-facilitates-viral-genome-tethering-and-membrane-penetration-for-mitotic-nuclear-entry
#6
Inci Aydin, Ruth Villalonga-Planells, Lilo Greune, Matthew P Bronnimann, Christine M Calton, Miriam Becker, Kun-Yi Lai, Samuel K Campos, M Alexander Schmidt, Mario Schelhaas
Incoming papillomaviruses (PVs) depend on mitotic nuclear envelope breakdown to gain initial access to the nucleus for viral transcription and replication. In our previous work, we hypothesized that the minor capsid protein L2 of PVs tethers the incoming vDNA to mitotic chromosomes to direct them into the nascent nuclei. To re-evaluate how dynamic L2 recruitment to cellular chromosomes occurs specifically during prometaphase, we developed a quantitative, microscopy-based assay for measuring the degree of chromosome recruitment of L2-EGFP...
May 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28288666/reliability-of-preoperative-evaluation-of-postmenopausal-ovarian-tumors
#7
Riikka Johanna Niemi, Sami Kristian Saarelainen, Tiina Hannele Luukkaala, Johanna Unelma Mäenpää
BACKGROUND: Preoperative evaluation of ovarian tumors is challenging. This study was undertaken to evaluate the performance of conventional two-dimensional (2D) ultrasound and CA125 in predicting malignant or benign nature of pelvic masses, and to investigate if three-dimensional power Doppler (3DPD) ultrasound provides any added value. Ninety-six postmenopausal and four perimenopausal women with supposed ovarian tumors were examined by standardized 2D and 3DPD ultrasounds preoperatively...
March 14, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28280379/amylmetacresol-2-4-dichlorobenzyl-alcohol-hexylresorcinol-or-carrageenan-lozenges-as-active-treatments-for-sore-throat
#8
Martina Morokutti-Kurz, Christine Graf, Eva Prieschl-Grassauer
Up to 80% of sore throats are caused by viruses. Several over the counter products are available which provide symptomatic, not causal relief. For such lozenges, containing the antiseptics and local anesthetics amylmetacresol (AMC) and 2,4-dichlorobenzyl alcohol (DCBA) or hexylresorcinol (HR), recently an additional virucidal effect was published. Therefore, we tested a set of Strepsils(®) lozenges, containing either HR (Max [#2]) or AMC/DCBA (Original [#3], Extra Strong [#4], Warm [#5], Orange and Vitamin C [#6], Sugar free Lemon [#7], Children/Strawberry [#8] and Soothing Honey and Lemon [#9]) for their antiviral efficiency against representatives of respiratory viruses known to cause sore throat: human rhinovirus (HRV) 1a, HRV8, influenza virus A H1N1n, Coxsackievirus A10, and human coronavirus (hCoV) OC43...
2017: International Journal of General Medicine
https://www.readbyqxmd.com/read/28271890/comparison-of-implicit-and-explicit-solvation-models-for-iota-cyclodextrin-conformation-analysis-from-replica-exchange-molecular-dynamics
#9
Wasinee Khuntawee, Manaschai Kunaseth, Chompoonut Rungnim, Suradej Intagorn, Peter Wolschann, Nawee Kungwan, Thanyada Rungrotmongkol, Supot Hannongbua
Large ring cyclodextrins have become increasingly important for drug delivery applications. In this work, we have performed replica-exchange molecular dynamics simulations using both implicit and explicit water solvation models to study the conformational diversity of iota-cyclodextrin containing 14 α-1,4 glycosidic linked d-glucopyranose units (CD14). The new quantifiable calculation methods are proposed to analyze the openness, bending, and twisted conformation of CD14 in terms of circularity, biplanar angle, and one-directional conformation (ODC)...
April 24, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28264336/the-genus-i-manota-i-williston-diptera-mycetophilidae-in-peruvian-amazonia-with-description-of-sixteen-new-species-and-notes-on-local-species-richness
#10
Heikki Hippa, Olavi Kurina, Ilari E Sääksjärvi
A comprehensive study of material of the worldwide fungus gnat genus Manota Williston, sampled from the Allpahuayo-Mishana National Reserve in Peruvian Amazonia, was conducted. The following 16 species are described as new: M. aligera sp. n., M. aristoseta sp. n., M. calva sp. n., M. ciliata sp. n., M. exigua sp. n., M. digitata sp. n., M. flabellata sp. n., M. iquitosensis sp. n., M. limulata sp. n., M. micella sp. n., M. minutula sp. n., M. nuda sp. n., M. parvula sp. n., M. pauloides sp. n., M. pustulosa sp...
February 21, 2017: Zootaxa
https://www.readbyqxmd.com/read/28250728/case-report-of-ovarian-torsion-mimicking-ovarian-cancer-as-an-uncommon-late-complication-of-laparoscopic-supracervical-hysterectomy
#11
Michał Ciebiera, Arkadiusz Baran, Aneta Słabuszewska-Jóźwiak, Grzegorz Jakiel
Laparoscopic supracervical hysterectomy (LSH) is an example of a partial hysterectomy, performed due to benign gynaecological complaints. Better endoscopic instruments and operational techniques have led to a great reduction in the number of abdominal hysterectomies. It is believed that LSH is a safe and minimally invasive hysterectomy technique. The Cochrane Database meta-analysis proves the benefits of minimally invasive surgery compared with abdominal gynaecological surgery, including decreased pain, surgical-site infections and hospital stay, quicker return to activity, and fewer postoperative adhesions...
December 2016: Przeglad Menopauzalny, Menopause Review
https://www.readbyqxmd.com/read/28215985/detection-of-clostridium-perfringens-toxin-genes-in-the-gut-microbiota-of-autistic-children
#12
Sydney M Finegold, Paula H Summanen, Julia Downes, Karen Corbett, Tomoe Komoriya
We studied stool specimens from 33 autistic children aged 2-9 years with gastrointestinal (GI) abnormalities and 13 control children without autism and without GI symptoms. We performed quantitative comparison of all Clostridium species and Clostridium perfringens strains from the fecal microbiota by conventional, selective anaerobic culture methods. We isolated C. perfringens strains and performed PCR analysis for the main C. perfringens toxin genes, alpha, beta, beta2, epsilon, iota and C. perfringens enterotoxin gene...
June 2017: Anaerobe
https://www.readbyqxmd.com/read/28210460/cloning-and-expression-of-clostridium-perfringens-type-d-vaccine-strain-epsilon-toxin-gene-in-e-coli-as-a-recombinant-vaccine-candidate
#13
Parastoo Aziminia, Reza Pilehchian-Langroudi, Kasra Esmaeilnia
BACKGROUND AND OBJECTIVES: Clostridium perfringens, a Gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. C. perfringens is subdivided into five types A to E based on its four major alpha, beta, epsilon and iota toxins. The aim of the present study was cloning and expression of C. perfringens type D vaccine strain epsilon toxin gene. MATERIALS AND METHODS: Genomic DNA was extracted and the epsilon toxin gene was amplified using Pfu DNA polymerase...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28199340/crystal-structure-and-structure-based-mutagenesis-of-actin-specific-adp-ribosylating-toxin-cpile-a-as-novel-enterotoxin
#14
Waraphan Toniti, Toru Yoshida, Toshiharu Tsurumura, Daisuke Irikura, Chie Monma, Yoichi Kamata, Hideaki Tsuge
Unusual outbreaks of food poisoning in Japan were reported in which Clostridium perfringens was strongly suspected to be the cause based on epidemiological information and fingerprinting of isolates. The isolated strains lack the typical C. perfringens enterotoxin (CPE) but secrete a new enterotoxin consisting of two components: C. perfringens iota-like enterotoxin-a (CPILE-a), which acts as an enzymatic ADP-ribosyltransferase, and CPILE-b, a membrane binding component. Here we present the crystal structures of apo-CPILE-a, NAD+-CPILE-a and NADH-CPILE-a...
2017: PloS One
https://www.readbyqxmd.com/read/28095404/entropy-transfer-between-residue-pairs-and-allostery-in-proteins-quantifying-allosteric-communication-in-ubiquitin
#15
Aysima Hacisuleyman, Burak Erman
It has recently been proposed by Gunasakaran et al. that allostery may be an intrinsic property of all proteins. Here, we develop a computational method that can determine and quantify allosteric activity in any given protein. Based on Schreiber's transfer entropy formulation, our approach leads to an information transfer landscape for the protein that shows the presence of entropy sinks and sources and explains how pairs of residues communicate with each other using entropy transfer. The model can identify the residues that drive the fluctuations of others...
January 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28077248/alternative-splicing-at-exon-2-results-in-the-loss-of-the-catalytic-activity-of-mouse-dna-polymerase-iota-in-vitro
#16
Konstantin Y Kazachenko, Nataliya A Miropolskaya, Leonid V Gening, Vyacheslav Z Tarantul, Alena V Makarova
Y-family DNA polymerase iota (Pol ι) possesses both DNA polymerase and dRP lyase activities and was suggested to be involved in DNA translesion synthesis and base excision repair in mammals. The 129 strain of mice and its derivatives have a natural nonsense codon mutation in the second exon of the Pol ι gene resulting in truncation of the Pol ι protein. These mice were widely used as a Pol ι-null model for in vivo studies of the Pol ι function. However whether 129-derived strains of mice are fully deficient in the Pol ι functions was a subject of discussion since Pol ι mRNA undergoes alternative splicing at exon 2...
February 2017: DNA Repair
https://www.readbyqxmd.com/read/28070856/mir-29c-prkci-regulates-axonal-growth-of-dorsal-root-ganglia-neurons-under-hyperglycemia
#17
Longfei Jia, Lei Wang, Michael Chopp, Chao Li, Yi Zhang, Alexandra Szalad, Zheng Gang Zhang
Diabetes initially induces distal axonal damage of peripheral nerves, but molecular mechanisms that mediate axonal injury are not fully understood. MircoRNAs (miRNAs) regulate axonal growth. We found that diabetic db/db mice exhibited substantial upregulation of miR-29c in dorsal root ganglia (DRG) neurons, sciatic nerve, and foot pad tissues. Bioinformatic analysis revealed PRKCI, a gene that encodes a member of the protein kinase C (PKC) iota, as a putative target for miR-29c. Western blot analysis showed that diabetic mice exhibited a considerable reduction of PRKCI protein levels in sciatic nerve tissues and DRG neurons...
January 9, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28004457/clinical-and-ultrasound-characteristics-of-surgically-removed-adnexal-lesions-with-a-largest-diameter-%C3%A2-2-5-cm-a-pictorial-essay
#18
A Di Legge, P Pollastri, R Mancari, M Ludovisi, F Mascilini, D Franchi, D Jurkovic, M E Coccia, D Timmerman, G Scambia, A Testa, L Valentin
OBJECTIVES: The aim was to describe the ultrasound characteristics, the indications for surgery and the histological diagnoses of surgically removed adnexal masses with largest diameter <2.5 cm ("very small tumors"), to estimate sensitivity and specificity with regard to malignancy of subjective assessment of ultrasound images of very small tumors, and to present a gallery of ultrasound images of surgically removed very small tumors especially those causing diagnostic difficulties...
December 22, 2016: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27995173/response-to-letter-to-the-editor-concerning-validation-of-iota-adnex-model
#19
Sebastian Szubert, Andrzej Wojtowicz, Patryk Zywica
No abstract text is available yet for this article.
November 2016: Gynecologic Oncology Reports
https://www.readbyqxmd.com/read/27936952/reliability-of-the-ninds-common-data-elements-cranial-tomography-ct-rating-variables-for-traumatic-brain-injury-tbi
#20
Leah Harburg, Erin McCormack, Kimbra Kenney, Carol Moore, Kelly Yang, Pieter Vos, Bram Jacobs, Christopher J Madden, Ramon Diaz-Arrastia, Tanya Bogoslovsky
BACKGROUND: Non-contrast head computer tomography (CT) is widely used to evaluate eligibility of patients after acute traumatic brain injury (TBI) for clinical trials. The NINDS Common Data Elements (CDEs) TBI were developed to standardize collection of CT variables. The objectives of this study were to train research assistants (RAs) to rate CDEs and then to evaluate their performance. The aim was to assess inter-rater reliability (IRR) of CDEs between trained RAs and a neurologist and to evaluate applicability of CDEs in acute and sub-acute TBI to test the feasibility of using CDE CT ratings in future trials and ultimately in clinical practice...
2017: Brain Injury: [BI]
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