Sandra P Santos, Yang Yang, Margarida T G Rosa, Mafalda A A Rodrigues, Claire Bouthier De La Tour, Suzanne Sommer, Miguel Teixeira, Maria A Carrondo, Peter Cloetens, Isabel A Abreu, Célia V Romão
The bacterium Deinococcus radiodurans is highly resistant to several stress conditions, such as radiation. According to several reports, manganese plays a crucial role in stress protection, and a high Mn/Fe ratio is essential in this process. However, mobilization of manganese and iron, and the role of DNA-binding-proteins-under-starved-conditions during oxidative-stress remained open questions. We used synchrotron-based X-ray fluorescence imaging at nano-resolution to follow element-relocalization upon stress, and its dependency on the presence of Dps proteins, using dps knockout mutants...
November 20, 2019: Scientific Reports