M Raquel Pacheco, João P Jacinto, Daniela Penas, Tomás Calmeiro, Ana V Almeida, Miriam Colaço, Elvira Fortunato, Nykola C Jones, Søren V Hoffmann, M Manuela A Pereira, Pedro Tavares, Alice S Pereira
A missense mutant of a Dps protein (DNA-binding protein from starved cells) from Marinobacter hydrocarbonoclasticus was used as a building block to develop a new supramolecular assembly complex which enhances the iron uptake, a physiological function of this mini-ferritin. The missense mutation was conducted in an exposed and flexible region of the N-terminal, wherein a threonine residue in position 10 was replaced by a cysteine residue (DpsT10C). This step enabled a click chemistry approach to the variant DpsT10C, where a thiol-ene coupling occurs...
November 25, 2020: Organic & Biomolecular Chemistry