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Tyrosinase

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https://www.readbyqxmd.com/read/29456684/capn3-dct-mlana-and-tyrp1-are-overexpressed-in-skin-of-vitiligo-vulgaris-mexican-patients
#1
Mauricio Salinas-Santander, Víctor Trevino, Eduardo De la Rosa-Moreno, Bárbara Verduzco-Garza, Celia N Sánchez-Domínguez, Cristina Cantú-Salinas, Jorge Ocampo-Garza, Armando Lagos-Rodríguez, Jorge Ocampo-Candiani, Rocio Ortiz-López
Vitiligo is a disorder causing skin depigmentation, in which several factors have been proposed for its pathogenesis: Environmental, genetic and biological aspects of melanocytes, even those of the surrounding keratinocytes. However, the lack of understanding of the mechanisms has complicated the task of predicting the development and progression. The present study used microarray analysis to characterize the transcriptional profile of skin from Vitiligo Vulgaris (VV) patients and the identified transcripts were validated using targeted high-throughput RNA sequencing in a broader set of patients...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29453917/comparative-chemical-investigation-and-evaluation-of-antioxidant-and-tyrosinase-inhibitory-effects-of-withania-somnifera-l-dunal-and-solanum-nigrum-l-berries
#2
Abdul Hameed, Naveed Akhtar
In the present study, berries of two different species of Solanaceae family, Withania somnifera (WS) and Solanum nigrum (SN), were extracted in methanol and then fractionated with solvents, ranging from non-polar to polar, for their phytochemical profiling and investigation of antioxidant and tyrosinase enzyme inhibition capacity. The methanolic extract and n-hexane, ethyl acetate (WSEA, SNEA) and aqueous fractions were chemically analyzed and evaluated for biological activity. Total flavonoids and total phenolics were quantified in WSEA (96...
March 1, 2018: Acta Pharmaceutica
https://www.readbyqxmd.com/read/29448823/chemical-constituents-of-genista-numidica-spach-aerial-parts-and-their-antimicrobial-antioxidant-and-antityrosinase-activities
#3
Naima Boutaghane, Abdulmagid Alabdul Magid, Amin Abedini, Anaïs Cafolla, Hanène Djeghim, Sophie C Gangloff, Laurence Voutquenne-Nazabadioko, Zahia Kabouche
A previously undescribed triterpenoid saponin, 3-O-[α-l-rhamnopyranosyl-(1→2)-{β-d-glucopyranosyl-(1→6)-}β-d-galactopyranosyl-(1→2)-β-d-glucuronopyranosyl]-sophoradiol (1), in addition to twenty-nine known constituents (2-30) were isolated from the aerial parts of Genista numidica Spach. Structures elucidation was performed by comprehensive 1D- and 2D-NMR analyses and HRESIMS. The extracts, fractions and isolated compounds were evaluated for their antibacterial, antioxidant and tyrosinase inhibitory activities...
February 15, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29448223/2d-transition-metal-carbide-mxene-as-a-robust-biosensing-platform-for-enzyme-immobilization-and-ultrasensitive-detection-of-phenol
#4
Lingxia Wu, Xianbo Lu, Dhanjai, Zhong-Shuai Wu, Yanfeng Dong, Xiaohui Wang, Shuanghao Zheng, Jiping Chen
MXene-Ti 3 C 2 , as a new class of two-dimensional (2D) transition metal carbides (or nitrides), has been synthesized by exfoliating pristine Ti 3 AlC 2 phases with hydrofluoric acid. The SEM and XRD images show that the resultant MXene possesses a graphene-like 2D nanostructure. and the surface of MXene has been partially terminated with -OH, thus providing a favorable microenvironment for enzyme immobilization and retaining their bioactivity and stability. Considering the unique metallic conductivity, biocompatibility and good dispersion in aqueous phase, the as-prepared MXene was explored as a new matrix to immobilize tyrosinase (a model enzyme) for fabricating a mediator-free biosensor for ultrasensitive and rapid detection of phenol...
February 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29448090/uhplc-qtof-ms-analysis-of-bioactive-constituents-from-two-romanian-goji-lycium-barbarum-l-berries-cultivars-and-their-antioxidant-enzyme-inhibitory-and-real-time-cytotoxicological-evaluation
#5
Andrei Mocan, Cadmiel Moldovan, Gökhan Zengin, Onur Bender, Marcello Locatelli, Mario Simirgiotis, Arzu Atalay, Dan C Vodnar, Sascha Rohn, Gianina Crișan
Goji (Lycium barbarum L.) berries are emphasized as healthy food or are used widely as dietary supplements. In the present study, the antioxidant and enzyme inhibitory activities of berries extracts from two selected Romanian cultivars (cv. Erma and cv. Biglifeberry) have been evaluated. UHPLC-QTOF-MS analysis results revealed the presence of vitamin C and related compounds, phenolic acids, flavonoids, fatty acids (oxylipins), and spermidine derivatives. In particular, cv. Erma showed a strong tyrosinase-inhibitory effect, whereas cv...
February 12, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29447970/development-of-a-new-paper-based-nano-biosensor-based-on-the-co-catalytic-effect-of-tyrosinase-from-banana-peel-tissue-musa-cavendish-and-functionalized-silica-nanoparticles-for-voltammetric-determination-of-l-tyrosine
#6
Mohadeseh Rahimi-Mohseni, Jahan Bakhsh Raoof, Reza Ojani, Tahereh A Aghajanzadeh, Ayemeh Bagheri Hashkavayi
A new and facile method for electrochemical determination of L-tyrosine was designed. Tyrosinase from banana peel tissue was immobilized at fiber matrices of the paper disc via physical adsorption. The mediator potassium hexacyanoferrate and 3-mercaptopropyl trimethoxysilane-functionalized silica nanoparticles were added to the paper disc, respectively. The modified paper disc was placed on the top of the graphite screen printed electrode and electrochemical characterization of this biosensor was studied by cyclic voltammetry and electrochemical impedance spectroscopy methods...
February 12, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29447969/novel-piperonal-1-3-4-thiadiazolium-2-phenylamines-mesoionic-derivatives-synthesis-tyrosinase-inhibition-evaluation-and-hsa-binding-study
#7
Natália Drumond Lopes, Otávio Augusto Chaves, Márcia C C de Oliveira, Carlos Mauricio R Sant'Anna, Danilo Sousa-Pereira, José Carlos Netto-Ferreira, Aurea Echevarria
A novel series of piperonal mesoionic derivatives (PMI 1-6) was synthesized. Tyrosinase inhibition in the presence of PMI-1, -2, -3, -4, -5 and -6 as well as human serum albumin (HSA) binding studies with PMI-5 and PMI-6 were done by spectroscopic and theoretical methods. The mesoionic compound PMI-5 is the most promising tyrosinase inhibitor with a noncompetitive inhibitory mechanism and an IC 50 = 124 μmol L -1 . In accordance with the kinetic profile, molecular docking results show that PMI-5 is able to interact favorably with the tyrosinase active site containing the substrate molecule, L-DOPA, interacting with Val-247, Phe-263 and Val-282 residues...
February 12, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29443872/ap-1-kif13a-blocking-peptides-impair-melanosome-maturation-and-melanin-synthesis
#8
Cécile Campagne, Léa Ripoll, Floriane Gilles-Marsens, Graça Raposo, Cédric Delevoye
Melanocytes are specialized cells that generate unique organelles called melanosomes in which melanin is synthesized and stored. Melanosome biogenesis and melanocyte pigmentation require the transport and delivery of melanin synthesizing enzymes, such as tyrosinase and related proteins (e.g., TYRP1), from endosomes to maturing melanosomes. Among the proteins controlling endosome-melanosome transport, AP-1 together with KIF13A coordinates the endosomal sorting and trafficking of TYRP1 to melanosomes. We identify here β1-adaptin AP-1 subunit-derived peptides of 5 amino acids that block the interaction of KIF13A with AP-1 in cells...
February 14, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29441618/enhanced-drug-photosafety-by-interchromophoric-interaction-due-to-intramolecular-charge-separation
#9
Ming-De Li, Zhiping Yan, Ruixue Zhu, David Lee Phillips, Isabel Aparici-Espert, Virginie Lhiaubet-Vallet, Miguel Angel Miranda
Imatinib is a tyrosinase inhibitor employed for the treatment of some kinds of human cancer. This drug has a low phototoxicity toward DNA but its pyridylpyrimidine (1) fragment by itself exhibits significant phototoxicitiy. The intrinsic mechanism that leads to the enhanced photosafety of Imatinib is not yet known. Here, the properties of the excited state and interchromophoric interactions of Imatinib have been explored using ultrafast laser flash photolysis and agarose electrophoresis studies. An intramolecular charge separation is directly observed upon the illuminated imatinib, which accounts for the relaxation of its excited state...
February 14, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29439519/biochemical-mechanism-of-rhododendrol-induced-leukoderma
#10
REVIEW
Shosuke Ito, Kazumasa Wakamatsu
-4-(4-hydroxyphenyl)-2-butanol (rhododendrol (RD))-a skin-whitening ingredient-was reported to induce leukoderma in some consumers. We have examined the biochemical basis of the RD-induced leukoderma by elucidating the metabolic fate of RD in the course of tyrosinase-catalyzed oxidation. We found that the oxidation of racemic RD by mushroom tyrosinase rapidly produces RD-quinone, which gives rise to secondary quinone products. Subsequently, we confirmed that human tyrosinase is able to oxidize both enantiomers of RD...
February 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29438574/protective-effects-of-an-extract-of-the-freshwater-microalga-scenedesmus-rubescens-on-uv-irradiated-skin-cells
#11
Remo Campiche, Petra Sandau, Elke Kurth, Marco Massironi, Dominik Imfeld, Rolf Schuetz
BACKGROUND: Skin aging results from intrinsic but also extrinsic factors of which UV-irradiation is a main cause. It is hence of interest to have means to protect skin from UV -irradiation induced damage. We selected an extract of the freshwater microalga Scenedesmus rubescens and assessed its potential to protect skin from photoaging caused by UV-irradiation. METHODS: Skin cells in-vitro and ex-vivo were analyzed for markers of UV-irradiation induced photodamage such as decreased viability, decreased collagen content, hyperpigmentation, and sunburn cells...
February 13, 2018: International Journal of Cosmetic Science
https://www.readbyqxmd.com/read/29436631/trp%C3%A2-2-mediates-coat-color-pigmentation-in-sheep-skin
#12
Linli Xue, Yanan Li, Bingling Zhao, Tianzhi Chen, Yanjun Dong, Ruiwen Fan, Jingwei Li, Haidong Wang, Xiaoyan He
Tyrosinase‑related protein 2 (TRP‑2) is one of the most important members of the tyrosinase family, and is a key enzyme involved in melanin biosynthesis. In the present study, a skin transcriptome profile, immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction were used to investigate TRP‑2 expression in sheep with different coat colors, namely, black, white and black‑white. TRP‑2 was overexpressed in melanocytes in order to study the effect of TRP‑2 on melanin production...
February 6, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29430350/bioactive-properties-of-chamaerops-humilis-l-antioxidant-and-enzyme-inhibiting-activities-of-extracts-from-leaves-seeds-pulp-and-peel
#13
Sandra Gonçalves, Joana Medronho, Elsa Moreira, Clara Grosso, Paula B Andrade, Patrícia Valentão, Anabela Romano
In this work we evaluated methanolic extracts from different parts (leaves, seeds, fruit peel and pulp) of Chamaerops humilis L. for antioxidant activity and the ability to inhibit enzymes linked with neurodegenerative diseases: acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (TYR). The total content of phenolics, flavonoids and condensed tannins was also determined. The antioxidant and inhibitory activities of the extracts varied significantly according to the tissue. Seed extracts showed the greatest ability to scavenge DPPH (IC50 = 81...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29428974/malignant-rhabdoid-tumors-originating-within-and-outside-the-central-nervous-system-are-clinically-and-molecularly-heterogeneous
#14
Emilia M Pinto, Dima Hamideh, Armita Bahrami, Brent A Orr, Tong Lin, Stanley Pounds, Gerard P Zambetti, Alberto S Pappo, Amar Gajjar, Sameer Agnihotri, Alberto Broniscer
Multifocal synchronous or metachronous atypical teratoid rhabdoid tumors (ATRTs) and non-central nervous system malignant rhabdoid tumors (extra-CNS MRTs) are rare cancers. We reviewed the clinical and radiologic characteristics of affected patients seen at our institution. Genotyping and analysis of copy number abnormalities (CNAs) in SMARCB1 were performed in germline and tumor samples. Tumor samples underwent genome-wide DNA methylation and CNA analysis. The median age at diagnosis of 21 patients was 0.6 years...
February 10, 2018: Acta Neuropathologica
https://www.readbyqxmd.com/read/29427719/antibacterial-activity-of-hemocyanin-from-red-swamp-crayfish-procambarus-clarkii
#15
Zhendong Qin, V Sarath Babu, Quanyuan Wan, Asim Muhammad, Jun Li, Jiangfeng Lan, Li Lin
Hemocyanins (HMC): the copper-containing respiratory proteins present in invertebrate hemolymph, which plays many essential roles in the immune system. Currently, little is known about the HMC domains of Procambarus clarkii (P. clarkii) and their function in antimicrobial immune response. In this present study, we comparatively studied the expression pattern of native PcHMC with the three recombinant proteins of variable domains of crayfish hemocyanin (PcHMC-N, N-terminal domain of hemocyanin; PcHMC-T, tyrosinase domain of hemocyanin; and PcHMC-C, C-terminal domain of hemocyanin)...
February 7, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29427586/inhibition-of-human-tyrosinase-requires-molecular-motifs-distinctively-different-from-mushroom-tyrosinase
#16
Tobias Mann, Wolfram Gerwat, Jan Batzer, Kerstin Eggers, Cathrin Scherner, Horst Wenck, Franz Stäb, Vincent J Hearing, Klaus-Heinrich Röhm, Ludger Kolbe
Tyrosinase is the rate-limiting enzyme of melanin production and, accordingly, is the most prominent target to inhibit hyperpigmentation. Numerous tyrosinase inhibitors have been identified, but most of those lack clinical efficacy because they were identified using mushroom tyrosinase as the target. Therefore, we used recombinant human tyrosinase to screen a library of 50,000 compounds and compared the active screening hits with well-known whitening ingredients. Hydroquinone and its derivative arbutin only weakly inhibited human tyrosinase with a half-maximal inhibitory concentration (IC50) in the millimolar range, while kojic acid showed a weak efficacy (IC50 > 400 μM)...
February 7, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29425653/in-vitro-and-in-silico-perspectives-on-biological-and-phytochemical-profile-of-three-halophyte-species-a-source-of-innovative-phytopharmaceuticals-from-nature
#17
Gokhan Zengin, Zaahira Aumeeruddy-Elalfi, Adriano Mollica, Mustafa Abdullah Yilmaz, Mohamad Fawzi Mahomoodally
BACKGROUND: Halophytes are considered as valuable sources of traditional drugs in different countries. PURPOSE: The present study aimed to evaluate biological and chemical fingerprints of three halophytes (Arthrocnemum macrostachyum (Moric.) C, Koch, Halimione portulacoides (L.) Aellen and Salicornia europaea L.). MATERIALS AND METHODS: The antioxidant and enzymatic inhibitory potential (acetylcholinesterase, butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase) were assessed...
January 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29425647/predicting-tyrosinase-inhibition-by-3d-qsar-pharmacophore-models-and-designing-potential-tyrosinase-inhibitors-from-traditional-chinese-medicine-database
#18
Hongwei Gao
BACKGROUND: Tyrosinase plays a key role in the formation of skin melanin. The excessive accumulation of skin melanin will cause the serious aesthetic problems for human beings. HYPOTHESIS/PURPOSE: To find the potent tyrosinase inhibitors using computational simulation from TCM Database@Taiwan. STUDY DESIGN: Inhibitors of tyrosinase have been thought as potential drugs for the decrease of melanin synthesis in the process of pigmentation. To develop new tyrosinase inhibitors, we performed a virtual screening from Traditional Chinese medicine (TCM) and Druglike Databases using the best 3D QSAR pharmacophore model as a 3D search query...
January 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29413752/development-of-radioiodine-labeled-acetaminophen-for-specific-high-contrast-imaging-of-malignant-melanoma
#19
Wen Jing Zhu, Masato Kobayashi, Kohei Yamada, Kodai Nishi, Kotaro Takahashi, Asuka Mizutani, Ryuichi Nishii, Leo G Flores, Naoto Shikano, Munetaka Kunishima, Keiichi Kawai
INTRODUCTION: Due to its poor prognosis, specific imaging for early detection of malignant melanoma is strongly desired. Although radioiodine-labeled 4-hydroxyphenylcysteamine, which we previously developed, has good affinity for tyrosinase, an enzyme in the melanin metabolic pathway, image contrast of the melanoma:organ ratios is not sufficiently high for detection of primary melanoma and metastases at early injection times. In this study, we developed radioiodine-labeled acetaminophen (I-AP) for specific, high-contrast imaging of malignant melanoma...
January 3, 2018: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/29408614/proteomic-analyses-for-profiling-regulated-proteins-enzymes-by-fucus-vesiculosus-fucoidan-in-b16-melanoma-cells-a-combination-of-enzyme-kinetics-functional-study
#20
Zhi-Jiang Wang, Li Zheng, Jun-Mo Yang, Yani Kang, Yong-Doo Park
Fucoidans are complex sulfated polysaccharides that have a wide range of biological activities. Previously, we reported the various effects of Fucus vesiculosus fucoidan on tyrosinase and B16 melanoma cells. In this study, to identify fucoidan-targeted proteins in B16 melanoma cells, we performed a proteomics study and integrated enzyme kinetics. We detected 19 candidate proteins dysregulated by fucoidan treatment. Among the probed proteins, the enzyme kinetics of two candidate enzymes, namely lactate dehydrogenase (LDH) as an upregulated protein and superoxide dismutase (SOD) as a downregulated enzyme, were determined...
February 3, 2018: International Journal of Biological Macromolecules
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