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https://www.readbyqxmd.com/read/29672960/thai-herbal-antipyretic-22-formula-apf22-inhibits-uva-mediated-melanogenesis-through-activation-of-nrf2-regulated-antioxidant-defense
#1
Tasanee Onkoksoong, Saowanee Jeayeng, Naravat Poungvarin, Saowalak Limsaengurai, Onusa Thamsermsang, Pinpat Tripatara, Pravit Akarasereenont, Uraiwan Panich
Thai herbal antipyretic 22 formula (APF22), a polyherbal formula, has been traditionally used to treat dermatologic problems including hyperpigmentation. Exposure of the skin to ultraviolet A (UVA) causes abnormal melanin production induced by photooxidative stress. This study thus aimed to investigate the protective effects of APF22 extracts and phenolic compounds, ferulic acid (FA), and gallic acid (GA; used as positive control and reference compounds), on melanogenesis through modulation of nuclear factor E2-related factor 2 (Nrf2) signaling and antioxidant defenses in mouse melanoma (B16F10) cells exposed to UVA...
April 19, 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29669854/synthetic-biology-based-cellular-biomedical-tattoo-for-detection-of-hypercalcemia-associated-with-cancer
#2
Aizhan Tastanova, Marc Folcher, Marius Müller, Gieri Camenisch, Aaron Ponti, Thomas Horn, Maria S Tikhomirova, Martin Fussenegger
Diagnosis marks the beginning of any successful therapy. Because many medical conditions progress asymptomatically over extended periods of time, their timely diagnosis remains difficult, and this adversely affects patient prognosis. Focusing on hypercalcemia associated with cancer, we aimed to develop a synthetic biology-inspired biomedical tattoo using engineered cells that would (i) monitor long-term blood calcium concentration, (ii) detect onset of mild hypercalcemia, and (iii) respond via subcutaneous accumulation of the black pigment melanin to form a visible tattoo...
April 18, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29667556/ultrasound-promoted-green-synthesis-docking-study-of-indole-spliced-thiadiazole-%C3%AE-amino-phosphonates-as-anticancer-agents-and-anti-tyrosinase-agents
#3
Anna Pratima G Nikalje, Pramod Gawhane, Shailee Tiwari, Jaiprakash Sangshetti, Manoj Damale
In this work, a new series of diethyl ((1H-indole-3-yl)((5-phenyl-1,3,4-thiadiazole-2- yl)amino)methyl)phosphonate derivatives 6(a-j) were designed and synthesized in Ultrasound by green protocol using Kabachnik-Fields reaction. The structures of the synthesized compounds were confirmed by spectral analysis such as elemental analyses, IR, 1H NMR, 13C NMR, 31P NMR and mass spectra. The synthesized compounds 6(a-j) were appraised for their in vitro anticancer activity against human cancer cell lines such as SK-MEL-2 (melanoma), IMR-32 (Neuroblastoma), HT-29(Colon) and also on normal murine embryonic fibroblast NIH/3T3 by Sulforhodamine B (SRB) assay, using Adriamycin as a standard drug...
April 17, 2018: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29663060/a-novel-fluorescent-biosensor-for-adrenaline-detection-and-tyrosinase-inhibitor-screening
#4
Ziping Liu, Shasha Liu
In this work, a novel simple fluorescent biosensor for the highly sensitive and selective detection of adrenaline was established. Firstly, water-soluble CuInS2 quantum dots (QDs) capped by L-Cys were synthesized via a hydrothermal synthesis method. Then, the positively charged adrenaline was assembled on the surface of CuInS2 QDs due to the electrostatic interactions and hydrogen bonding, which led to the formation of adrenaline-CuInS2 QD (Adr-CuInS2 QD) electrostatic complexes. Tyrosinase (TYR) can catalyze adrenaline to generate H2 O2 , and additionally oxidize the adrenaline to adrenaline quinone...
April 17, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29658579/identification-of-tyr-mutations-in-patients-with-oculocutaneous-albinism
#5
Wan Sun, Yanjie Shen, Shan Shan, Liyun Han, Yang Li, Zheng Zhou, Zilin Zhong, Jianjun Chen
Oculocutaneous albinism (OCA) is a set of autosomal recessive disorders characterized by hypopigmented hair, skin and eyes. Homozygous or compound heterozygous mutations in the tyrosinase (TYR) gene can cause OCA1, which is the most common and severe subtype of albinism. In the present study, 17 patients with non‑syndromic OCA were enrolled from eight provinces of China and were non‑consanguineous, with the exception of Patient 4000301. Total genomic DNA was isolated from peripheral blood. Screening was performed for the whole exons and their flanking regions of the TYR gene using Sanger sequencing and the pathogenicity of variants was predicted using in silico analysis...
April 13, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29656242/a-highly-sensitive-electrochemical-biosensor-for-phenol-derivatives-using-a-graphene-oxide-modified-tyrosinase-electrode
#6
Yue Wang, Fengge Zhai, Yasushi Hasebe, Hongmin Jia, Zhiqiang Zhang
The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5×10-8 M to 5×10-5 M, a lower detection limit of 3×10-8 M, a current maximum (Imax ) of 65...
April 9, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29642633/structural-requirements-of-alkylglyceryl-l-ascorbic-acid-derivatives-for-melanogenesis-inhibitory-activity
#7
Norihisa Taira, Yushi Katsuyama, Masato Yoshioka, Osamu Muraoka, Toshio Morikawa
l-Ascorbic acid has multifunctional benefits on skin aesthetics, including inhibition of melanin production, and is widely used in cosmetics. It, however, has low stability and poor skin penetration. We hypothesize that alkylglyceryl-l-ascorbic acid derivatives, highly stable vitamin C-alkylglycerol conjugates, would have similar anti-melanogenic activity with better stability and penetration. We test 28 alkylglyceryl-l-ascorbic acid derivatives ( 1 - 28 ) on theophylline-stimulated B16 melanoma 4A5 cells to determine if they inhibit melanogenesis and establish any structure-function relationships...
April 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29642438/sesamol-inhibited-melanogenesis-by-regulating-melanin-related-signal-transduction-in-b16f10-cells
#8
Po-Yuan Wu, Ya-Jhen You, Yi-Jung Liu, Chien-Wei Hou, Chin-Sheng Wu, Kuo-Ching Wen, Chien-Yih Lin, Hsiu-Mei Chiang
Melanin is synthesized through a series of interactions catalyzed by melanogenic enzymes such as tyrosinase, dopachrome tautomerase (tyrosinase-related protein-2; TRP-2), and tyrosinase-related protein-1 (TRP-1). Tyrosinase plays a key role in catalysing the initial and limiting steps of melanogenesis. The melanin that results from melanogenesis has the protective effect of absorbing ultraviolet radiation. However, overproduction of melanin, in addition to altering the appearance of skin, may lead to skin disorders such as melasma, solar lentigo, and postinflammatory hyperpigmentation...
April 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29625258/chemical-characterization-with-in-vitro-biological-activities-of-gypsophila-species
#9
Dimitrina Zheleva-Dimitrova, Gokhan Zengin, Vessela Balabanova, Yulian Voynikov, Valentin Lozanov, Irina Lazarova, Reneta Gevrenova
Methanol-aqueous extracts from the aerial parts of Gypsophila glomerata (GGE), G. trichotoma (GTE) and G. perfoliata (GPE) were investigated for antioxidant potential using different in vitro models, as well as for phenolic and flavonoid contents. The possible anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glucosidase activities were also tested. The flavonoid variability was analyzed using ultra high-performance liquid chromatography (UHPLC) coupled with hybrid quadrupole-Orbitrap high resolution mass spectrometry (HRMS)...
March 21, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29624845/neuroprotective-potential-and-chemical-profile-of-alternatively-cultivated-ganoderma-lucidum-basidiocarps
#10
Jasmina Lj Ćilerdžić, Ivana V Sofrenić, Vele V Tešević, Ilija D Brčeski, Sonja N Duletić-Laušević, Jelena B Vukojević, Mirjana M Stajić
Various neurodegenerative diseases are the main challenges to the modern medicine and there is a great need for novel, natural, neuroprotective agents. Ganoderma lucidum is a well-known medicinal mushroom which health benefits have been confirmed by numerous studies. As demand for its basidiocarps is increased and traditional cultivation on hardwoods is not environmentally-friendly and economically-justified, finding of alternative substrates is necessary. The aim of the study was to assess the effect of alternative cultivation substrates on the chemical profile of G...
April 6, 2018: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29621941/anti-melanogenic-effects-of-resorcinol-are-mediated-by-suppression-of-camp-signaling-and-activation-of-p38-mapk-signaling
#11
Mingyeong Kang, See-Hyoung Park, Sae Woong Oh, Seung Eun Lee, Ju Ah Yoo, Youn Hwa Nho, Sukyeon Lee, Byung Seok Han, Jae Youl Cho, Jongsung Lee
In this study, we investigated the inhibitory mechanisms of resorcinol in B16F10 mouse melanoma cells. We found that resorcinol reduced both the melanin content and tyrosinase activity in these cells. In addition, resorcinol suppressed the expression of melanogenic gene microphthalmia-associated transcriptional factor (MITF) and its downstream target genes tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2. In addition, we found that resorcinol reduced intracellular cAMP levels and protein kinase A (PKA) activity, and increased phosphorylation of the p38 mitogen-activated protein kinase (MAPK)...
April 5, 2018: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29621499/new-insight-into-the-allosteric-effect-of-l-tyrosine-on-mushroom-tyrosinase-during-l-dopa-production
#12
Sorour Hassani, Behzad Gharechaei, Somayeh Nikfard, Mostafa Fazli, Nematollah Gheibi, Renaud Hardré, Raymond L Legge, Kamahldin Haghbeen
Kinetics studies of L-tyrosine (LTy) ortho-hydroxylation by mushroom tyrosinase (MT) confirmed that MT was severely, but not completely, inhibited at higher concentrations of LTy. Despite the availability of the crystal structure reports, no allosteric site has been identified on MT. To examine the assumption that a non-specific binding site works as a regulatory site, docking simulations were run for the second molecule of L-tyrosine (LTy2 ) on the complexes of the first L-tyrosine molecule (LTy1 ) with the heavy chain (H) of MT (LTy1 /HMT) and its dimer with the light chain (Ty1 /LHMT)...
April 2, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29614034/novel-1-e-3-e-5-e-1-6-bis-substituted-phenyl-hexa-1-3-5-triene-analogs-inhibit-melanogenesis-in-b16f10-cells-and-zebrafish
#13
Jisun Oh, Jungeun Kim, Jin Ho Jang, Sangwoo Lee, Chul Min Park, Woo-Keun Kim, Jong-Sang Kim
The present study aimed to evaluate the anti-melanogenic activity of 1,6-diphenyl-1,3,5-hexatriene and its derivatives in B16F10 murine melanoma cells and zebrafish embryos. Twenty five (1 E ,3 E ,5 E )-1,6- bis (substituted phenyl)hexa-1,3,5-triene analogs were synthesized and their non-cytotoxic effects were predictively analyzed using three-dimensional quantitative structure-activity relationship approach. Inhibitory activities of these synthetic compounds against melanin synthesis were determined by evaluating melanin content and melanogenic regulatory enzyme expression in B16F10 cells...
April 3, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29609010/artemisia-asiatica-ethanol-extract-exhibits-anti-photoaging-activity
#14
Deok Jeong, Jongsung Lee, Seong-Gu Jeong, Yo Han Hong, Sulgi Yoo, Sang Yun Han, Ji Hye Kim, Sunggyu Kim, Jin Sic Kim, Young Soo Chung, Jong-Hoon Kim, Young-Su Yi, Jae Youl Cho
ETHNOPHARMACOLOGICAL RELEVANCE: Artemisia asiatica Nakai is a traditional herbal plant that has long been used in anti-inflammatory, anti-infective and skin protective remedies. AIM OF THE STUDY: In this study, traditionally known skin-protective activity of Artemisia asiatica Nakai was examined with its ethanol extract (Aa-EE) under various photoaging conditions using skin-originated cells, and the underlying mechanism was also examined using various types of cells...
March 30, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29606116/successful-production-of-genome-edited-rats-by-the-rgonad-method
#15
Tomoe Kobayashi, Masumi Namba, Takayuki Koyano, Masaki Fukushima, Masahiro Sato, Masato Ohtsuka, Makoto Matsuyama
BACKGROUND: Recent progress in development of the CRISPR/Cas9 system has been shown to be an efficient gene-editing technology in various organisms. We recently developed a novel method called Genome-editing via Oviductal Nucleic Acids Delivery (GONAD) in mice; a novel in vivo genome editing system that does not require ex vivo handling of embryos, and this technology is newly developed and renamed as "improved GONAD" (i-GONAD). However, this technology has been limited only to mice...
April 2, 2018: BMC Biotechnology
https://www.readbyqxmd.com/read/29603875/toll-like-receptor-2-and-3-enhance-melanogenesis-and-melanosome-transport-in-human-melanocytes
#16
AutSaaya Koike, Kenshi Yamasaki, Takeshi Yamauchi, Mai Inoue, Ryoko Shimada-Ohmori, Kenichiro Tsuchiyama, Setsuya Aiba
Because little is known about how the innate immune response influences skin pigmentation, we examined whether Toll-like receptor (TLR) agonists participate in melanogenesis and melanosome transportation. We observed that TLR2/2 agonist HKLM and TLR3 agonist Poly(I:C) increased the amount of extracellular melanin from primary human epidermal melanocytes. HKLM, but not Poly(I:C), increased the melanogenic genes such as tyrosinase and dopachrome tautomerase. Poly(I:C) increased the expression of Rab27A, a molecule that facilitates melanosome transport to perimembranous actin filament...
March 30, 2018: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/29602773/pd-l1-independent-mechanisms-control-the-resistance-of-melanoma-to-cd4-t-cell-adoptive-immunotherapy
#17
Stephen R Goding, Kyle A Wilson, Carolyn Rosinsky, Paul Andrew Antony
Immunotherapy is becoming the standard of care for melanoma. However, resistance to therapy is a major problem. Previously, we showed that tumor-specific, cytotoxic CD4+ T cells from tyrosinase-related protein 1 transgenic mice could overcome secondary resistance to recurring melanoma when anti-programmed cell death 1 ligand (PD-L1) checkpoint blockade was combined with either anti-lymphocyte-activated gene 3 (LAG-3) Abs or depletion of tumor-specific regulatory T (Treg ) cells. In this study, we show that PD-L1 expressed by the host, not B16 melanoma, plays a major role in the early stages of exhaustion or primary resistance...
March 30, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29602084/a-comprehensive-lc-ms-ms-method-validation-for-the-quantitative-investigation-of-37-fingerprint-phytochemicals-in-achillea-species-a-detailed-examination-of-a-coarctata-and-a-monocephala
#18
Mustafa Abdullah Yilmaz, Abdulselam Ertas, Ismail Yener, Mehmet Akdeniz, Oguz Cakir, Muhammed Altun, Ibrahim Demirtas, Mehmet Boga, Hamdi Temel
The current study aims to optimize and validate a comprehensive LC-MS/MS method for the quantification of 37 phytochemicals (15 phenolic acids, 17 flavonoids, 3 non-phenolic organic acids, 1 phenolic aldehyde and 1 benzopyrene) in Achillea species. Though Achillea species were chosen as real life samples, the current method is applicable to a wide range of plant species. The developed method was fully validated in terms of linearity, accuracy (recovery), inter-day and intra-day precision (repeatability), limits of detection and quantification (LOD/LOQ) and relative standard uncertainty (U% at 95% confidence level (k = 2))...
March 10, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29597932/synthesis-and-antitumor-antityrosinase-and-antioxidant-activities-of-xanthone
#19
Bei-Dou Zhou, Li-Lan Zeng, Yu-Gui Tong, Jia-Ying Fang, Zhi-Peng Ruan, Xiao-Yun Zeng, Yuan-Yuan Fang, Gui-Fen Xu, Dong-Bao Hu
Ten substituted 1,3-dihydroxyxanthones were synthesized in one step. The yields ranged from 40 to 76%. Compounds 8-10 were first reported. Next, the compounds' in vitro anti-proliferative activities against nine human cancer cell lines, antityrosinase, and antioxidant activities were evaluated. Compounds 1, 4, 6-7, and 9-10 exhibited enhanced cytotoxicity against certain cancer cells. Compounds 2, 8, 9, and 10 inhibited tyrosinase activity to a certain extent. In addition, compound 4 exhibited the best antioxidant activity, which was consistent with theoretical calculations...
March 30, 2018: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/29597026/adhesive-gland-transcriptomics-uncovers-a-diversity-of-genes-involved-in-glue-formation-in-marine-tube-building-polychaetes
#20
Jean-Philippe Buffet, Erwan Corre, Evelyne Duvernois-Berthet, Jérôme Fournier, Pascal Jean Lopez
Tube-building sabellariid polychaetes are hermatypic organisms capable of forming vast reefs in highly turbulent marine habitats. Sabellariid worms assemble their tube by gluing together siliceous and calcareous clastic particles using a polyelectrolytic biocement. Here, we performed transcriptomic analyses to investigate the genes that are differentially expressed in the parathorax region, which contains the adhesive gland and tissues, from the rest of the body. We found a large number of candidate genes to be involved in the composition and formation of biocement in two species: Sabellaria alveolata and Phragmatopoma caudata...
March 26, 2018: Acta Biomaterialia
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