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https://www.readbyqxmd.com/read/28748655/high-throughput-automated-microbial-bioreactor-system-used-for-clone-selection-and-rapid-scale-down-process-optimisation
#1
M Lourdes Velez-Suberbie, John Pj Betts, Kelly L Walker, Colin Robinson, Barney Zoro, Eli Keshavarz-Moore
High throughput automated fermentation systems have become a useful tool in early bioprocess development. In this study, we investigated a 24 x 15 mL single use microbioreactor system, ambr 15f, designed for microbial culture. We compared the fed-batch growth and production capabilities of this system for two Escherichia coli strains, BL21 (DE3) and MC4100, and two industrially relevant molecules, hGH and scFv. In addition, different carbon sources were tested using bolus, linear or exponential feeding strategies, showing the capacity of the ambr 15f system to handle automated feeding...
July 27, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28748371/acute-effects-of-heated-resistance-exercise-in-female-and-male-power-athletes
#2
Julia R Casadio, Adam G Storey, Fabrice Merien, Andrew E Kilding, James D Cotter, Paul B Laursen
PURPOSE: To determine the effects of heated resistance exercise on thermal strain, neuromuscular function and hormonal responses in power athletes. METHODS: Sixteen (n = 8 female; 8 male) highly trained power athletes completed a combined strength and power resistance exercise session in hot (HOT ~30 °C) and temperate (CON ~20 °C) conditions. Human growth hormone (hGH), cortisol and testosterone concentrations in plasma, peak power (counter-movement jump, CMJ) and peak force (isometric mid-thigh pull) were measured before and after each training session; thermoregulatory responses were monitored during training...
July 26, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28730965/efficient-non-invasive-plasmid-dna-administration-into-tibialis-cranialis-muscle-of-little-mice
#3
C R Cecchi, E Higuti, E R Lima, D P Vieira, P L Squair, C N Peroni, P Bartolini
An alternative treatment for growth hormone deficiency based on hGH-DNA administration, followed by electro gene transfer, was investigated by injecting the plasmid into surgically exposed or non-exposed quadriceps or tibialis muscle of immunodeficient "little" mice. Previously optimized electrotransfer conditions were also improved via a new combination of high/low voltage pulses: after 3 days, serum hGH was determined. Both groups exhibited similar results: 5.0 ± 2.2 (SD) and 3.5 ± 0.9 ng hGH/ml (P>0...
July 21, 2017: Current Molecular Medicine
https://www.readbyqxmd.com/read/28724261/development-of-a-sandwich-enzyme-linked-immunosorbent-assay-for-dtmp-gh-fusion-protein-by-rational-immunogen-selection
#4
Song Wang, Mingqiang Shen, Shilei Chen, Cheng Wang, Fang Chen, Mo Chen, Gaomei Zhao, Xinze Ran, Tianmin Cheng, Yongping Su, Yang Xu, Junping Wang
dTMP-GH is a chimeric protein containing a tandem dimer of thrombopoietin mimetic peptide (dTMP) fused to human growth hormone (hGH) prepared previously by our team. It shows significant bioactivity in promoting thrombocytopoiesis, but detection of intact dTMP-GH in plasma is still a challenge due to the presence of endogenous hGH. In this study, a rabbit polyclonal antibody with high affinity to dTMP was obtained with a BSA-conjugated immunogen composed of 20 amino acids sequence spanning two TMP and the linker...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28720174/selective-determination-of-human-growth-hormone-somatropin-in-the-presence-of-its-chemical-degradation-products
#5
Mamdouh R Rezk, Marwa F Mohamed, Faten Abdelaziz Fathalla, Mostafa A Shehata
A rapid and sensitive HPLC method was developed and validated for selective determination of the human growth hormone (hGH) somatropin in the presence of its deamidated and oxidized degradation products. Reversed-phase chromatography with an acetonitrile and ammonium bicarbonate mobile phase in gradient elution mode was used. A short run time of 15 min allowed rapid and cost-effective analysis, with an average retention time of 7.4 min for native hGH, 6.2 min for its deamidated form, and 4.3 and 6 min for its oxidized variants...
July 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28700563/the-relationship-between-serum-levels-of-prolactin-and-growth-hormone-in-the-early-postnatal-period
#6
Jonathan Daliot, Tami Laron-Kenet, Mohammad Wattad, Anat Ben-Dor, Pearl Lilos, Zvi Laron
BACKGROUND: In the neonatal period, pituitary hormones including prolactin (PRL) and growth hormone (hGH) are secreted in high amounts due to immature feedback mechanisms. As both hormones are secreted in part by the same somatomammotrophic cells, we investigated their relationship in newborns in relation to sex, gestational week, method of delivery and anthropometric data. METHODS: Serum levels of PRL and hGH were measured in blood from 225 newborns. Newborn data were extracted from medical records...
July 12, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28659813/human-peritoneal-mesothelial-cell-death-induced-by-high-glucose-hypertonic-solution-involves-ca-2-and-na-ions-and-oxidative-stress-with-the-participation-of-pkc-nox2-and-pi3k-akt-pathways
#7
Felipe Simon, Pablo Tapia, Ricardo Armisen, Cesar Echeverria, Sebastian Gatica, Alejandro Vallejos, Alejandro Pacheco, Maria E Sanhueza, Miriam Alvo, Erico Segovia, Rubén Torres
Chronic peritoneal dialysis (PD) therapy is equally efficient as hemodialysis while providing greater patient comfort and mobility. Therefore, PD is the treatment of choice for several types of renal patients. During PD, a high-glucose hyperosmotic (HGH) solution is administered into the peritoneal cavity to generate an osmotic gradient that promotes water and solutes transport from peritoneal blood to the dialysis solution. Unfortunately, PD has been associated with a loss of peritoneal viability and function through the generation of a severe inflammatory state that induces human peritoneal mesothelial cell (HPMC) death...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28656605/somapacitan-a-once-weekly-reversible-albumin-binding-gh-derivative-in-children-with-gh-deficiency-a-randomized-dose-escalation-trial
#8
Tadej Battelino, Michael Højby Rasmussen, Jean De Schepper, Nehama Zuckerman-Levin, Zoran Gucev, Lars Sävendahl
OBJECTIVE: To evaluate the safety, local tolerability, pharmacodynamics and pharmacokinetics of escalating single doses of once-weekly somapacitan, a reversible, albumin-binding GH derivative, versus once-daily GH in children with GH deficiency (GHD). DESIGN: Phase 1, randomized, open-label, active-controlled, dose-escalation trial (NCT01973244). PATIENTS: Thirty-two pre-pubertal GH-treated children with GHD were sequentially randomized 3:1 within each of four cohorts to a single dose of somapacitan (0...
June 28, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28617312/autocrine-human-growth-hormone-promotes-invasive-and-cancer-stem-cell-like-behavior-of-hepatocellular-carcinoma-cells-by-stat3-dependent-inhibition-of-claudin-1-expression
#9
Yi-Jun Chen, Ming-Liang You, Qing-Yun Chong, Vijay Pandey, Qiu-Shi Zhuang, Dong-Xu Liu, Lan Ma, Tao Zhu, Peter E Lobie
Despite progress in diagnosis and treatment of hepatocellular carcinoma (HCC), the clinical outcome is still unsatisfactory. Increased expression of human growth hormone (hGH) in HCC has been reported and is associated with poor survival outcome in HCC patients. Herein, we investigated the mechanism of the oncogenic effects of hGH in HCC cell lines. In vitro functional assays demonstrated that forced expression of hGH in these HCC cell lines promoted cell proliferation, cell survival, anchorage-independent growth, cell migration, and invasion, as previously reported...
June 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28611730/exploiting-nanobodies-in-the-detection-and-quantification-of-human-growth-hormone-via-phage-sandwich-enzyme-linked-immunosorbent-assay
#10
Hossam Murad, Jana Mir Assaad, Rasha Al-Shemali, Abdul Qader Abbady
BACKGROUND: Monitoring blood levels of human growth hormone (hGH) in most children with short stature deficiencies is crucial for taking a decision of treatment with extended course of daily and expensive doses of recombinant hGH (rhGH or Somatropin(®)). Besides, misusing of rhGH by sportsmen is banned by the World Anti-Doping Agency and thus sensitive GH-detecting methods are highly welcome in this field. Nanobodies are the tiniest antigen-binding entity derived from camel heavy chain antibodies...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28595133/nonclinical-pharmacokinetic-and-pharmacodynamic-characterisation-of-somapacitan-a-reversible-non-covalent-albumin-binding-growth-hormone
#11
Peter Thygesen, Henrik Sune Andersen, Carsten Behrens, Johannes Josef Fels, Leif Nørskov-Lauritsen, Christian Rischel, Nils Langeland Johansen
OBJECTIVE: Somapacitan is an albumin-binding growth hormone derivative intended for once weekly administration, currently in clinical development for treatment of adult as well as juvenile GH deficiency. Nonclinical in vivo pharmacological characterisation of somapacitan was performed to support the clinical trials. Here we present the pharmacokinetic and pharmacodynamic effects of somapacitan in rats, minipigs, and cynomolgus monkeys. METHODS: Pharmacokinetic studies investigating exposure, absorption, clearance, and bioavailability after single intravenous (i...
May 24, 2017: Growth Hormone & IGF Research
https://www.readbyqxmd.com/read/28588001/successful-growth-hormone-therapy-in-cornelia-de-lange-syndrome
#12
Michael de Graaf, Sarina G Kant, Jan Maarten Wit, Egbert Johan Willem Redeker, Gijs Willem Eduard Santen, Annemieke Johanna Maria Henriëtta Verkerk, André Gerardus Uitterlinden, Monique Losekoot, Wilma Oostdijk
Cornelia de Lange Syndrome (CdLS) is a heterogeneous syndrome, both clinically and genetically, in its classical form characterised by distinctive facial features, intra-uterine growth retardation, short stature, developmental delay and anomalies in multiple organ systems. NIPBL, SMC1A, SMC3, RAD21 and HDAC8, all involved in the Cohesin pathway, have been identified to cause CdLS. Growth hormone (GH) secretion has been reported as normal, and to our knowledge there are no reports on the effect of recombinant human GH (r-hGH) treatment in CdLS patients...
June 7, 2017: Journal of Clinical Research in Pediatric Endocrinology
https://www.readbyqxmd.com/read/28551577/bioactive-growth-hormone-in-older-men-and-women-it-s-relationship-to-immune-markers-and-healthspan
#13
William J Kraemer, Mary J Kennett, Andrea M Mastro, Roger J McCarter, Connie J Rogers, William H DuPont, Shawn D Flanagan, William J Turbitt, Maren S Fragala, Emily M Post, Wesley C Hymer
OBJECTIVE: The consequences of age-related decline in the somatotropic axis of humans are complex and remain largely unresolved. We tested the hypothesis that hGH measurements of plasma by bioassay vs immunoassay from samples obtained from free-living, elderly individuals would reveal a dichotomy in GH activities that are correlated with the functional status of the donors, i.e. their healthspan. DESIGN: Forty-one men and women of advanced age (men: N=16, age, 80...
June 2017: Growth Hormone & IGF Research
https://www.readbyqxmd.com/read/28546545/extensive-phenotypic-characterization-of-a-new-transgenic-mouse-reveals-pleiotropic-perturbations-in-physiology-due-to-mesenchymal-hgh-minigene-expression
#14
Aimilios Kaklamanos, Jan Rozman, Manolis Roulis, Niki Karagianni, Maria Armaka, Moya Wu, Laura Brachthäuser, Julia Calzada-Wack, Marion Horsch, Johannes Beckers, Birgit Rathkolb, Thure Adler, Frauke Neff, Eckhard Wolf, Valerie Gailus-Durner, Helmut Fuchs, Martin Hrabe de Angelis, George Kollias
The human growth hormone (hGH) minigene used for transgene stabilization in mice has been recently identified to be locally expressed in the tissues where transgenes are active and associated with phenotypic alterations. Here we extend these findings by analyzing the effect of the hGH minigene in TgC6hp55 transgenic mice which express the human TNFR1 under the control of the mesenchymal cell-specific CollagenVI promoter. These mice displayed a fully penetrant phenotype characterized by growth enhancement accompanied by perturbations in metabolic, skeletal, histological and other physiological parameters...
May 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28537648/an-anterior-inferior-iliac-spine-avulsion-fracture-in-a-14-year-old-boy-on-human-growth-hormone
#15
Anthony J Scillia, Todd P Pierce, Anthony Festa, Peter Goljan, Vincent K McInerney
We present the case of a young man with an anterior inferior iliac spine (AIIS) avulsion fracture who was taking human growth hormone (HGH) at the time his injury was diagnosed. He presented with chronic hip pain and no traumatic event. Physical exam revealed symptoms of hip impingement. Upon imaging, he was diagnosed with an AIIS avulsion fracture and underwent surgical repair. He returned to full activity six months post-operatively. At 18-months post-operatively, he was pain-free and performing all activities without difficulty...
May 24, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/28525404/growth-hormone-treatment-for-growth-hormone-deficiency-and-idiopathic-short-stature-new-guidelines-shaped-by-the-presence-and-absence-of-evidence
#16
Adda Grimberg, David B Allen
PURPOSE OF REVIEW: The Pediatric Endocrine Society recently published new guidelines for the use of human growth hormone (hGH) and human insulin-like growth factor-I (hIGF-I) treatment for growth hormone deficiency, idiopathic short stature, and primary IGF-I deficiency in children and adolescents. This review places the new guidelines in historical contexts of the life cycle of hGH and the evolution of US health care, and highlights their future implications. RECENT FINDINGS: The new hGH guidelines, the first to be created by the Grading of Recommendations Assessment, Development and Evaluation approach, are more conservative than their predecessors...
August 2017: Current Opinion in Pediatrics
https://www.readbyqxmd.com/read/28515770/high-level-expression-of-recombinant-human-growth-hormone-in-escherichia-coli-crucial-role-of-translation-initiation-region
#17
Mahsa Ghavim, Khalil Abnous, Fatemeh Arasteh, Sahar Taghavi, Maryam Sadat Nabavinia, Mona Alibolandi, Mohammad Ramezani
For high-throughput production of recombinant protein in Escherichia coli (E. coli), besides important parameters such as efficient vector with strong promoter and compatible host, other important issues including codon usage, rare codons, and GC content specially at N-terminal region should be considered. In the current study, the effect of decreasing the percentage of GC nucleotides and optimizing codon usage at N-terminal region of human growth hormone (hGH) cDNA on the level of its expression in E. coli were investigated...
April 2017: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28503764/interpretation-of-seemingly-contradictory-data-low-nmr-s-2-order-parameters-observed-in-helices-and-high-nmr-s-2-order-parameters-in-disordered-loops-of-the-protein-hgh-at-low-ph
#18
Lorna J Smith, Roya Athill, Wilfred F van Gunsteren, Niels Hansen
At low pH, human growth hormone (hGH) adopts a partially folded state, in which the native helices are maintained, but the long loop regions and side-chain packing become disordered. Some of the S(2) order parameters for backbone N-H vectors derived from NMR relaxation measurements on hGH at low pH initially seem contradictory. Three isolated residues (15, 20, and 171) in helices A and D exhibit low order parameter values (<0.5) indicating flexibility, whereas residue 143 in the centre of a long flexible loop region has a high order parameter (0...
May 15, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28427901/the-20kda-and-22kda-forms-of-human-growth-hormone-hgh-exhibit-different-intracellular-signalling-profiles-and-properties
#19
Liu Yao-Xia, Chen Jing-Yan, Tang Xia-Lian, Chen Ping, Zhang Min
Human Growth Hormone (hGH) includes two main variants. The first is 22kDa GH (22K-GH), which is predominant in the blood circulation. The second most abundant variant is 20K-GH, which makes up 5-10% of the blood circulation. Both bind and activate the same receptor, called the human growth hormone receptor (GHR). However, the reason why 22K-GH and 20K-GH exhibit similar, but not identical physiological activities remains poorly understood. In this article, the intracellular signalling profiles between these two hormones were examined...
April 17, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28421307/an-efficient-and-rapid-method-to-monitor-the-oxidative-degradation-of-protein-pharmaceuticals-probing-tyrosine-oxidation-with-fluorogenic-derivatization
#20
Rupesh Bommana, Olivier Mozziconacci, Y John Wang, Christian Schöneich
PURPOSE: The loss of potency of protein therapeutics can be linked to the oxidation of specific amino acid residues leading to a great variety of oxidative modifications. The comprehensive identification of these oxidative modifications requires high-resolution mass spectrometry analysis, which requires time and expensive resources. Here, we propose a fluorogenic derivatization method of oxidized Tyr and Phe yielding benzoxazole derivatives, as an orthogonal technique for the rapid screening of protein oxidation...
April 18, 2017: Pharmaceutical Research
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