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2013: Kidney International Supplements
(no author information available yet)
No abstract text is available yet for this article.
2013: Kidney International Supplements
Yanli Yang, Jun Ma, Liyun Zhao
No abstract text is available yet for this article.
September 4, 2018: Journal of Critical Care
Nudrat Noor, Geng Zong, Ellen W Seely, Marc Weisskopf, Tamarra James-Todd
BACKGROUND: Low to moderate acute cadmium exposure has been associated with increased risk of chronic diseases, such as cardiovascular and kidney disease. Little is known about the association between urinary cadmium levels-an indicator of longer-term exposure-and metabolic syndrome (MetS). METHODS: We analysed data from 3982 participants aged 20-<80 years of the National Health and Nutrition Examination Survey 2001-2014. Urinary cadmium levels were measured and adjusted for creatinine using spot urine samples...
September 19, 2018: Environment International
RuiHua Dong, JingSi Chen, JianHeng Zheng, MeiRu Zhang, Han Zhang, Min Wu, ShuGuang Li, Bo Chen
The effect of human exposure to phthalates and consequent contribution to the development of cardiometabolic health problems is unknown. However, oxidative stress has been established as playing an important role in the pathogenesis of cardiometabolic outcomes. In this study, we aimed to explore whether exposure to phthalate metabolites could induce cardiometabolic risk by increasing oxidative stress in a diabetic population from Shanghai. We collected paired blood and urine samples from a total of 300 volunteers, and measured 10 phthalate metabolites in urine and biomarkers of oxidative stress from serum including glucose and lipid levels, and liver and kidney damage...
September 19, 2018: Environment International
Matheus Credendio Eiras, Cristiana Libardi Miranda-Furtado, Artur Fernandes, Ester Silveira Ramos
Birds have a ZZ and ZW sex chromosome system (male and female, respectively). On the short arm of the Z chromosome of Gallus gallus domesticus there is a repetitive region called MHM region, which is absent on the W chromosome, that causes a natural copy number difference of the MHM region between the sexes, making possible the development of a quantitative PCR (qPCR) sexing assay, based on the Z chromosome double dose. Twenty-seven samples of tissues from eight adult Gallus gallus domesticus (four males and four females) were used to establish the parameters of the MHM copy-number sexing assay...
September 15, 2018: Theriogenology
Lina Shan, Bingjun Bai, Yiming Lv, Binbin Xie, Xuefeng Huang, Hongbo Zhu
Peritoneal metastasis from colorectal cancer (CRC) is related to poor prognosis. Intraperitoneal chemotherapy is an efficient method to treat peritoneal metastasis (PM); however, the outcomes remain unsatisfactory. The present study aimed to investigate the antitumor activity of lobaplatin and its role in intraperitoneal chemotherapy. The findings showed that the proliferation of CRC was inhibited in a dose-dependent manner when DLD1 and HCT116 cells were treated with various concentrations of lobaplatin (0, 6...
September 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Entsar R Mokhtar, Eman M, Maha A Hassan, Hanaa A Abou-ELHassan, Mona G Alanany, Mohammed A Hasuna
Renal dysfunction is a key risk factor for all-cause mortality in patients with type 2 diabetes (T2D). Special attention has been raised regarding the role of soluble tumor necrosis factor receptor1 (sTNFR1) and brain natriuretic peptide (BNP) in diabetic nephropathy (DN) since they play a crucial role in the pathogenesis of T2D complications. Elevated concentrations of sTNFR1and BNP were found to be associated with progression to end stage renal disease (ESRD) in T2D. We determined serum levels of sTNFR1 and BNP in T2D patients and correlated them with various clinical variables especially kidney function and urinary albumin creatinine ratio (UACR)...
January 2018: Egyptian Journal of Immunology
Hari Prasad, Donna K Dang, Kalyan C Kondapalli, Niranjana Natarajan, Valeriu Cebotaru, Rajini Rao
We observed significant and selective up-regulation of the Na+ /H+ exchanger NHA2 (SLC9B2) in cysts of patients with autosomal dominant polycystic kidney disease. Using the MDCK model of cystogenesis, we find NHA2 increases cyst size. Silencing or pharmacological inhibition of NHA2 inhibits cyst formation in vitro. Polycystin-1 represses NHA2 expression via Ca2+ /NFAT signalling whereas the dominant negative membrane-anchored C-terminal fragment (PC1-MAT) increased NHA2 levels. Drugs (caffeine, theophylline) and hormones (vasopressin, aldosterone) known to exacerbate cysts elicit NHA2 expression...
September 22, 2018: Journal of Physiology
R J Chance, G A Cameron, M Fordyce, P Noguera, T Wang, C Collins, C J Secombes, B Collet
Fish are the second most widely utilized vertebrate group used for scientific procedures in the United Kingdom, but the development and application of 3Rs (the principles of replacement, reduction, and refinement) in aquaculture disease research lags behind methodologies in place for mammalian studies. With a need for individual monitoring and non-lethal sampling, the effect of repeat anaesthesia on experimental fish needs to be better understood. This study to analyses the effect of repeat anaesthesia with MS-222, metomidate and AQUI-S upon the gill and general health of post-smolt Atlantic salmon Salmo salar...
September 22, 2018: Journal of Fish Biology
Laurine Robert, Grégoire Ficheur, Bertrand Décaudin, Juliette Gellens, Michel Luyckx, Renaud Perichon, Sophie Gautier, François Puisieux, Emmanuel Chazard, Jean-Baptiste Beuscart
Drug-induced hyperkalemia is a frequent and severe complication in hospital setting. Other risk factors may also induce hyperkalemia but the combination of drugs and precipitating factors has not been extensively studied. The aim was to identify drug-induced hyperkalemia events in hospitalized older patients and to describe their combinations with precipitating factors. Two experts analyzed independently retrospective data of patients aged 75 years or more. Experts identified 471 hyperkalemia events and concluded that 379 (80...
September 22, 2018: Clinical Pharmacology and Therapeutics
Syuan-Hao Syu, Yung-Wei Lin, Ke-Hsun Lin, Liang-Ming Lee, Chi-Hao Hsiao, Yu-Ching Wen
Immunosuppressive therapies decreased the incidence of acute kidney rejection after kidney transplantation, but also increased the risk of infections and sepsis. This study aimed to identify the risk factors associated with complications and/or graft failure in kidney transplant patients with sepsis. A total of 14,658 kidney transplant patients with sepsis, identified in the National Inpatient Sample (NIS) database (data from 2005-2014), were included in the study and classified into three groups: patients without complications or graft failure/dialysis (Group 1), patients with complications only (Group 2), and patients with complications and graft failure/dialysis (Group 3)...
September 16, 2018: Bosnian Journal of Basic Medical Sciences
Tia A Arena, Peter D Harms, Athena W Wong
Transient transfection of mammalian cells is used in the biotechnology industry to quickly supply recombinant protein for research and large molecule drug development. Here, we describe a method for high throughput transient transfection of Human Embryonic Kidney 293 (HEK293) cells in 30 mL tubespins using polyethylenimine (PEI) as a transfection reagent. An automated liquid handler can be used to perform pipetting steps for transfecting batches of 96 tubespins, and septa in the tubespin caps allow for rapid processing without decapping...
2018: Methods in Molecular Biology
David L Hacker, Laurence Durrer, Soraya Quinche
Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of about 15 mL and 400 mL, respectively.
2018: Methods in Molecular Biology
Denis L'Abbé, Louis Bisson, Christian Gervais, Eric Grazzini, Yves Durocher
Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection process of suspension-growing human embryo kidney 293 cells using two commercially available serum-free media and polyethylenimine as the transfection reagent...
2018: Methods in Molecular Biology
Hang-Kun Ma, Yue Liu, Bo Li, Ying Zhang, Lin-Juan Sun, Feng-Qin Xu
OBJECTIVE: To evaluate the efficacy of oral Chinese medicine (CM) in comparison with donepezil, a cholinesterase inhibitor (ChEI), for the treatment of Alzheimer's disease (AD). METHODS: Randomized controlled trials (RCTs) have been searched, and the effect of CM compared with donepezil in AD has been investigated. An electronic search of MEDLINE, Excerpta Medica Database (EMBASE), Cochrane Library, Chinese Biological Medicine Database (CBMdisc), and China National Knowledge Infrastructure (CNKI) to identify articles in English and Chinese from the inception of the database until October 18, 2015...
September 22, 2018: Chinese Journal of Integrative Medicine
Satoru Mizushiri, Makoto Daimon, Hiroshi Murakami, Aya Kamba, Sho Osonoi, Masato Yamaichi, Koki Matsumura, Jutaro Tanabe, Yuki Matsuhashi, Miyuki Yanagimachi, Itoyo Tokuda, Shizuka Kurauchi, Kaori Sawada
Association between serum calcium (Ca) levels and kidney dysfunction progression in a non-chronic kidney disease (CKD) population has not been well elucidated, especially in consideration for classical metabolic risk conditions such as hypertension, dyslipidemia, and diabetes, and those related to Ca metabolism. Among participants of the population-based Iwaki study of Japanese people, those with an estimated glomerular filtration rate (eGFR) ≧60 ml/min/1.73 m2 and age ≧40 years, and who attended the study consecutively in 2014 and 2015 were enrolled (gender (M/F): 218/380; age: 58...
September 21, 2018: Scientific Reports
Harilaos Filippakis, Amine Belaid, Brian Siroky, Constance Wu, Nicola Alesi, Thomas Hougard, Julie Nijmeh, Hilaire C Lam, Elizabeth P Henske
Tuberous Sclerosis Complex (TSC), a rare genetic disorder with mechanistic target of rapamycin complex 1 (mTORC1) hyperactivation, is characterized by multi-organ hamartomatous benign tumors including brain, skin, kidney, and lung (Lymphangioleiomyomatosis). mTORC1 hyperactivation drives metabolic reprogramming including glucose and glutamine utilization, protein, nucleic acid and lipid synthesis. To investigate the mechanisms of exogenous nutrients uptake in Tsc2-deficient cells, we measured dextran uptake, a polysaccharide internalized via macropinocytosis...
September 21, 2018: Scientific Reports
Sunita P Ho, Ling Chen, Frances I Allen, Ryan S Hsi, Alex R Shimotake, Scott V Wiener, Misun Kang, Andrew M Minor, Marshall L Stoller
Nephrocalcinosis often begins on a calcium phosphate deposit, at the tip of the medullo-papillary complex (MPC) known as Randall's plaque (RP). Contextualizing proximally observed biominerals within the MPC has led us to postulate a mechanobiological switch that can trigger interstitial biomineralization at the MPC tip, remote from the intratubular biominerals. Micro X-ray computed tomography scans of human MPCs correlated with transmission and scanning electron micrographs, and X-ray energy dispersive spectrometry demonstrated novel findings about anatomically-specific biominerals...
September 21, 2018: Scientific Reports
Omar Alkandari, Lieuko Nguyen, Diane Hebert, Valerie Langlois, Natasha A Jawa, Rulan S Parekh, Lisa A Robinson
BACKGROUND AND OBJECTIVES: AKI is associated with progression of CKD. Little is known about AKI after kidney transplantation in pediatric recipients. We aim to describe the epidemiology, risk factors, consequences, and outcomes of AKI in this population. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We performed a retrospective longitudinal analysis of pediatric kidney transplant recipients followed at The Hospital for Sick Children (Toronto, Canada) from 2001 to 2012...
September 21, 2018: Clinical Journal of the American Society of Nephrology: CJASN
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