keyword
https://read.qxmd.com/read/38532596/potential-of-the-quorum-quenching-and-plant-growth-promoting-halotolerant-bacillus-toyonensis-aa1ec1-as-biocontrol-agent
#21
JOURNAL ARTICLE
Amalia Roca, Mónica Cabeo, Carlos Enguidanos, Fernando Martínez-Checa, Inmaculada Sampedro, Inmaculada Llamas
The use of fertilizers and pesticides to control plant diseases is widespread in intensive farming causing adverse effects together with the development of antimicrobial resistance pathogens. As the virulence of many Gram-negative phytopathogens is controlled by N-acyl-homoserine lactones (AHLs), the enzymatic disruption of this type of quorum-sensing (QS) signal molecules, mechanism known as quorum quenching (QQ), has been proposed as a promising alternative antivirulence therapy. In this study, a novel strain of Bacillus toyonensis isolated from the halophyte plant Arthrocaulon sp...
March 2024: Microbial Biotechnology
https://read.qxmd.com/read/38532340/overexpression-of-osduf6-increases-salt-stress-tolerance-in-rice
#22
JOURNAL ARTICLE
Guangming Ma, Yong Zhang, Xiangyang Li
BACKGROUND: Soil salinity is one of the primary environmental stresses faced in rice production. When plants are exposed to salt stress, a series of cellular balances will be disrupted. Dufulin is an immune-induced antiviral agent used in plants. The DUF gene family influences plant response to abiotic stress, and the functional role of OsDUF6(ABA98726.1) in rice response to salt stress is being investigated here. RESULTS: Based on the transcriptome analysis of Dufulin treatment in inducing salt tolerance in rice, we selected the OsDUF6 protein located on the cell membrane and studied its molecular function by overexpressing OsDUF6...
March 26, 2024: BMC Plant Biology
https://read.qxmd.com/read/38532311/investigation-of-the-morphological-physiological-biochemical-and-catabolic-characteristics-and-gene-expression-under-drought-stress-in-tolerant-and-sensitive-genotypes-of-wild-barley-hordeum-vulgare-subsp-spontaneum-k-koch-asch-graebn
#23
JOURNAL ARTICLE
Hooman Shirvani, Ali Ashraf Mehrabi, Mohsen Farshadfar, Hooshmand Safari, Ali Arminian, Foad Fatehi, Alireza Pouraboughadareh, Peter Poczai
BACKGROUND: Barley (H. vulgare L.) is an important cereal crop cultivated across various climates globally. Barley and its ancestor (H. vulgare subsp. spontaneum) are an economically valuable model for genetic research and improvement. Drought, among various abiotic stresses, is a substantial threat to agriculture due to its unpredictable nature and significant impact on crop yield. RESULTS: This study was conducted in both greenhouse and laboratory settings. Prior to the study, wild barley accessions were pre-selected based on their sensitivity or tolerance to drought as determined from fieldwork in the 2020-2021 and 2021-2022 cropping seasons...
March 26, 2024: BMC Plant Biology
https://read.qxmd.com/read/38532219/removal-of-high-lead-concentration-by-hydroponic-cultures-of-normal-and-transformed-plants-of-scirpus-americanus-pers
#24
JOURNAL ARTICLE
Elizabeth Esquivel-Ramos, Ma Catalina Alfaro-de la Torre, María Del Socorro Santos-Díaz
Lead is a very toxic metal which affects human health. An alternative to remove it from contaminated water is the use of macrophytes, as Scirpus americanus Pers. This species is tolerant to salt and metals and has high biomass. The present research analyzed the capacity of hydroponic cultures of normal and transgenic plants (line T12) from S. americanus to remove high concentrations of lead. The antioxidant response of plants to metal exposure was also measured. The MINTEQ3.1 program was used to define the media composition in order to have the metal available to the plants...
March 27, 2024: Environmental Science and Pollution Research International
https://read.qxmd.com/read/38532186/acute-toxicity-and-anti-enterotoxigenic-activity-of-pigment-extracted-from-micrococcus-roseus
#25
JOURNAL ARTICLE
Mahmoud Yolmeh, Morteza Khomeiri, Ezzatollah Ghaemi, Mehmet Selim Şilbir
Microbial pigments are considered as one of the main sources of natural types, and the attention to them is increasing in the food and pharmaceutical industries. This study aimed to investigate the effects of pigments extracted from Micrococcus roseus (PEM) on the gene expression of a and b staphylococcal enterotoxins (sea and seb) and their acute toxicity. Real-time PCR was used to study the anti-enterotoxigenic activity of PEM against Staphylococcus aureus at sub-inhibitory concentrations. In addition, the acute toxicity of PEM was evaluated on albino mice through alkaline phosphatase (ALP), aspartate aminotransferas (AST), and alanine aminotransferase (ALT) of liver and its histopathological changes...
March 27, 2024: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/38531969/deciphering-functional-roles-of-protein-succinylation-and-glutarylation-using-genetic-code-expansion
#26
JOURNAL ARTICLE
Maria Weyh, Marie-Lena Jokisch, Tuan-Anh Nguyen, Maximilian Fottner, Kathrin Lang
Post-translational modifications (PTMs) dynamically regulate cellular processes. Lysine undergoes a range of acylations, including malonylation, succinylation (SucK) and glutarylation (GluK). These PTMs increase the size of the lysine side chain and reverse its charge from +1 to -1 under physiological conditions, probably impacting protein structure and function. To understand the functional roles of these PTMs, homogeneously modified proteins are required for biochemical studies. While the site-specific encoding of PTMs and their mimics via genetic code expansion has facilitated the characterization of the functional roles of many PTMs, negatively charged lysine acylations have defied this approach...
March 26, 2024: Nature Chemistry
https://read.qxmd.com/read/38531509/the-effect-of-enzymatic-and-viability-dye-treatment-in-combination-with-long-range-pcr-on-assessing-tulane-virus-infectivity
#27
JOURNAL ARTICLE
Sarah M Stoppel, Bjørn Tore Lunestad, Mette Myrmel
Human norovirus (HuNoV) is regularly involved in food-borne infections. To detect infectious HuNoV in food, RT-qPCR remains state of the art but also amplifies non-infectious virus. The present study combines pre-treatments, RNase and propidium monoazide, with three molecular analyses, including long-range PCR, to predominantly detect infectious Tulane virus (TuV), a culturable HuNoV surrogate. TuV was exposed to inactivating conditions to assess which molecular method most closely approximates the reduction in infectious virus determined by cell culture (TCID50 )...
March 24, 2024: Journal of Virological Methods
https://read.qxmd.com/read/38531206/assessing-combined-effects-of-long-term-exposure-to-copper-and-marine-heatwaves-on-the-reef-forming-serpulid-ficopomatus-enigmaticus-through-a-biomarker-approach
#28
JOURNAL ARTICLE
Verdiana Vellani, Alessia Cuccaro, Matteo Oliva, Carlo Pretti, Monia Renzi
Sessile benthic organisms can be affected by global changes and local pressures, such as metal pollution, that can lead to damages at different levels of biological organization. Effects of exposure to marine heatwaves (MHWs) alone and in combination with environmentally relevant concentration of copper (Cu) were evaluated in the reef-forming tubeworm Ficopomatus enigmaticus using a multi-biomarker approach. Biomarkers of cell membrane damage, enzymatic antioxidant defences, metabolic activity, neurotoxicity, and DNA integrity were analyzed...
March 25, 2024: Marine Pollution Bulletin
https://read.qxmd.com/read/38531187/evaluation-of-the-operational-conditions-of-the-glucose-oxidase-and-catalase-multienzymatic-system-through-enzyme-co-immobilization-on-amino-hierarchical-porous-silica
#29
JOURNAL ARTICLE
Tamara Galaz, Carminna Ottone, Karen Rodríguez-Núñez, Claudia Bernal
Hexaric acids have attracted attention lately because they are platform chemicals for synthesizing pharmaceuticals. In particular, gluconic acid is one of the most studied because it is readily available in nature. In this work, operational conditions like temperature and pH were evaluated for the enzymatic production of gluconic acid. For this purpose, glucose oxidase (GOx) and catalase (CAT) were individually immobilized and co-immobilized using amino-silica as support. The catalytic performance of the enzymes both as separate biocatalysts (GOx or CAT) and as an enzymatic complex (GOx-CAT) was assessed in terms of enzymatic activity and stability at temperatures 45 °C and 50 °C and pH 6 to 8...
March 19, 2024: Carbohydrate Research
https://read.qxmd.com/read/38531133/biotransformation-characteristics-of-tetracycline-by-strain-serratia-marcescens-msm2304-and-its-mechanism-evaluation-based-on-products-analysis-and-genomics
#30
JOURNAL ARTICLE
Siyu Wang, Jie Han, Ziyi Ge, Xu Su, Yixuan Chen, Jun Meng
Microbial biotransformation is a recommended and reliable method in face of formidable tetracycline (TC) with broad-spectrum antibacterial activity. Herein, comprehensive characteristics of a newfound strain and its molecular mechanism in process of TC bioremediation were involved in this study. Specifically, Serratia marcescens MSM2304 isolated from pig manure sludge grew well in presence of TC and achieved optimal removal efficiency of 61% under conditions of initial TC concentration of 10 mg/L, pH of 7...
March 25, 2024: Journal of Environmental Management
https://read.qxmd.com/read/38530809/spindle-checkpoint-activation-by-fungal-orthologs-of-the-s-cerevisiae-mps1-kinase
#31
JOURNAL ARTICLE
Amy Fabritius, Anabel Alonso, Andrew Wood, Shaheen Sulthana, Mark Winey
There is an ongoing need for antifungal agents to treat humans. Identification of new antifungal agents can be based on screening compounds using whole cell assays. Screening compounds that target a particular molecule is possible in budding yeast wherein sophisticated strain engineering allows for controlled expression of endogenous or heterologous genes. We have considered the yeast Mps1 protein kinase as a reasonable target for antifungal agents because mutant or druggable forms of the protein, upon inactivation, cause rapid loss of cell viability...
2024: PloS One
https://read.qxmd.com/read/38530542/the-inhibition-mechanisms-of-three-structurally-different-salvianolic-acids-on-the-non-enzymatic-glycation-of-bovine-serum-albumin
#32
JOURNAL ARTICLE
Guo Feng, Yu Yan, Mengfan Wang, Zhao Gao, Yinan Zhao, Xin Peng
The antiglycation mechanisms of three structurally different salvianolic acids (Sals) including salvianolic acid A (Sal-A), salvianolic acid B (Sal-B) and salvianolic acid C (Sal-C) were investigated using the bovine serum albumin (BSA)-fructose model. The results showed that the three compounds could inhibit the formation of glycation products, maintain protein structural stability, mitigate the development of amyloid fibrils and scavenge radicals. Notably, Sal-A possessed the highest anti-glycated activity compared with Sal-B and Sal-C...
March 26, 2024: Plant Foods for Human Nutrition
https://read.qxmd.com/read/38530416/cuco-2-o-4-nanoneedle-arrays-growth-on-carbon-cloth-as-a-non-enzymatic-electrochemical-sensor-with-low-detection-limit-ketoprofen-recognition
#33
JOURNAL ARTICLE
Yan Liu, Yuying Xin, Xin Wang, Xianfa Zhang, Yingming Xu, Xiaoli Cheng, Shan Gao, Lihua Huo
An electrochemical sensor for detecting ketoprofen was constructed by in-situ grown copper cobaltate (CuCo2 O4 ) nanoneedle arrays on a carbon cloth (CC) substrate. The resulting porous nanoneedle arrays not only expose numerous electrochemically active sites but also significantly enhance the electrochemical apparent active area and current transmission efficiency. By leveraging its electrochemical properties, the sensor achieves an impressive detection limit for ketoprofen of 0.7 pM, with a linear range spanning from 2 pM ~ 2 µM...
March 26, 2024: Mikrochimica Acta
https://read.qxmd.com/read/38530342/emergence-of-catalytic-function-in-prebiotic-information-coding-polymers
#34
JOURNAL ARTICLE
Alexei V Tkachenko, Sergei Maslov
Life as we know it relies on the interplay between catalytic activity and information processing carried out by biological polymers. Here we present a plausible pathway by which a pool of prebiotic information-coding oligomers could acquire an early catalytic function, namely sequence-specific cleavage activity. Starting with a system capable of non-enzymatic templated replication, we demonstrate that even non-catalyzed spontaneous cleavage would promote proliferation by generating short fragments that act as primers...
March 26, 2024: ELife
https://read.qxmd.com/read/38530250/enzymatic-activity-of-cgas-in-the-presence-of-three-types-of-dnas-limited-cgas-stimulation-by-single-stranded-hiv-1-sl2-dna
#35
JOURNAL ARTICLE
Mineyuki Mizuguchi, Niko Kyan, Suzuka Nishimata, Yuko Nabeshima, Takayuki Obita
Cyclic GMP-AMP (cGAMP) synthase (cGAS) is activated by binding to DNA. Activated cGAS produces 2'3'-cGAMP, which subsequently binds to the adaptor protein STING (stimulator of interferon genes). This interaction triggers the cGAS/STING signaling pathway, leading to the production of type I interferons. Three types of DNA, namely double-stranded DNA longer than 40 base pairs, a 70-nucleotide single-stranded HIV-1 DNA known as SL2, and Y-form DNA with unpaired guanosine trimers (G3 Y-form DNA), induce interferon production by activating cGAS/STING signaling...
March 26, 2024: Bioscience Reports
https://read.qxmd.com/read/38530222/enzymes-as-indispensable-markers-in-disease-diagnosis
#36
REVIEW
Shehabaz Usman Pathan, Akash Kharwar, Madaje Amir Ibrahim, Shashi Bala Singh, Priyanka Bajaj
Enzymes have been used for disease diagnosis for many decades; however, advancements in technology like ELISA and flow cytometry-based detection have significantly increased their use and have increased the sensitivity of detection. Technological advancements in recombinant enzyme production have increased enzymatic stability, and the use of colorimetric-based and florescence-based assays has led to their increased use as biomarkers for disease detection. Enzymes like acid phosphatase, cathepsin, lactate dehydrogenase, thymidine kinase and creatine kinase are indispensable markers for diagnosing cancer, cardiovascular diseases and others...
March 26, 2024: Bioanalysis
https://read.qxmd.com/read/38530114/cysteine-independent-crispr-associated-protein-labeling-for-presentation-and-co-delivery-of-molecules-toward-genetic-and-epigenetic-regulations
#37
JOURNAL ARTICLE
Sadiya Tanga, Arpita Hota, Arkadeep Karmakar, Paramita Banerjee, Basudeb Maji
Labeling of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) associated proteins (Cas) is a huge challenge for their genome engineering applications. Cysteine-mediated bioconjugation is the most efficient strategy for labeling Cas proteins. Introducing a cysteine residue in the protein at the right place might be challenging without perturbing the enzymatic activity. We report a method that does not require cysteine residues for small molecule presentation on the CRISPR-associated protein SpCas9 for in vitro protein detection, probing cellular protein expression, and nuclear co-delivery of molecules in mammalian cells...
March 26, 2024: Chembiochem: a European Journal of Chemical Biology
https://read.qxmd.com/read/38530063/-not-available
#38
REVIEW
Liyan Jia, Shan Gao, Yan Qiao
Liquid-liquid phase separation (LLPS) is responsible for the emergence of intracellular membrane-less organelles and the development of coacervate protocells. Benefitting from the advantages of simplicity, precision, programmability, and noninvasiveness, light has become an effective tool to regulate the assembly dynamics of LLPS, and mediate various biochemical processes associated with LLPS. In this review, recent advances in optically controlling membrane-less organelles within living organisms are summarized, thereby modulating a series of biological processes including irreversible protein aggregation pathologies, transcription activation, metabolic flux, genomic rearrangements, and enzymatic reactions...
March 26, 2024: Small Methods
https://read.qxmd.com/read/38529951/nanoarchitectonics-of-bimetallic-cu-co-doped-nitrogen-carbon-nanozyme-functionalized-hydrogel-with-nir-responsive-phototherapy-for-synergistic-mitigation-of-drug-resistant-bacterial-infections
#39
JOURNAL ARTICLE
Sheng-Nan Song, Xin-Liu Zhao, Xiao-Chan Yang, Yong Ding, Feng-Di Ren, Xue-Yao Pang, Bo Li, Ji-Yuan Hu, Yu-Zhen Chen, Wei-Wei Gao
Superbug infections and transmission have become major challenges in the contemporary medical field. The development of novel antibacterial strategies to efficiently treat bacterial infections and conquer the problem of antimicrobial resistance (AMR) is extremely important. In this paper, a bimetallic CuCo-doped nitrogen-carbon nanozyme-functionalized hydrogel (CuCo/NC-HG) has been successfully constructed. It exhibits photoresponsive-enhanced enzymatic effects under near-infrared (NIR) irradiation (808 nm) with strong peroxidase (POD)-like and oxidase (OXD)-like activities...
March 26, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38529932/asparaginyl-endopeptidase-mediated-peptide-cyclization-for-phage-display
#40
JOURNAL ARTICLE
Xiao-Cui Wan, Yan-Ni Zhang, Hua Zhang, Ying Chen, Zhi-Hui Cui, Wen-Jing Zhu, Ge-Min Fang
We report here an enzymatic strategy for asparaginyl endopeptidase-mediated peptide cyclization. Incorporation of chloroacetyl groups into the recognition sequence of Oa AEP1 enabled intramolecular cyclization with Cys residues. Combining this strategy and phage display, we identified nanomolar macrocyclic peptide ligands targeting TEAD4. One of the bicyclic peptides binds to TEAD4 with a K D value of 139 nM, 16 times lower than its linear analogue, demonstrating the utility of this platform in discovering high-affinity macrocyclic peptide ligands...
March 26, 2024: Organic Letters
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