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https://www.readbyqxmd.com/read/28631624/printing-induced-cell-injury-evaluation-during-laser-printing-of-3t3-mouse-fibroblasts
#1
Zhengyi Zhang, Wenxuan Chai, Ruitong Xiong, Lei Zhou, Yong Huang
Three-dimensional bioprinting has emerged as a promising solution for the freeform fabrication of living cellular constructs, which can be used for tissue/organ transplantation and tissue models. During bioprinting, some living cells are unavoidably injured and may become necrotic or apoptotic cells. This study aims to investigate the printing-induced cell injury and evaluates injury types of post-printing cells using the annexin V/7-aminoactinomycin D and FAM-DEVD-FMK/propidium iodide assays during laser printing of NIH 3T3 mouse fibroblasts...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28628156/-atlantoaxial-reduction-and-fixation-guided-by-the-intraoperative-ct
#2
(no author information available yet)
OBJECTIVE: To evaluate the clinical result of atlantoaxial reduction and fixation guided by the intraoperative CT. METHODS: Sixteen cases were retrospectively studied, including seven males and nine females, with the mean age of 49.9 years. Twelve cases were diagnosed as chronic atlantoaxial instability or dislocation, while four cases as acute odontoid fracture and dislocation. Among the sixteen cases, fourteen underwent atlantoaxial fusion, while two underwent temporary atlantoaxial fixation without fusion...
June 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28626968/3d-printing-all-aromatic-polyimides-using-mask-projection-stereolithography-processing-the-nonprocessable
#3
Maruti Hegde, Viswanath Meenakshisundaram, Nicholas Chartrain, Susheel Sekhar, Danesh Tafti, Christopher B Williams, Timothy E Long
High-performance, all-aromatic, insoluble, engineering thermoplastic polyimides, such as pyromellitic dianhydride and 4,4'-oxydianiline (PMDA-ODA) (Kapton), exhibit exceptional thermal stability (up to ≈600 °C) and mechanical properties (Young's modulus exceeding 2 GPa). However, their thermal resistance, which is a consequence of the all-aromatic molecular structure, prohibits processing using conventional techniques. Previous reports describe an energy-intensive sintering technique as an alternative technique for processing polyimides with limited resolution and part fidelity...
June 19, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28619543/circumferential-three-dimensional-printed-tracheal-grafts-research-model-feasibility-and-early-results
#4
Faiz Y Bhora, Erik E Lewis, Sadiq S Rehmani, Adil Ayub, Wissam Raad, Adnan M Al-Ayoubi, Robert S Lebovics
BACKGROUND: Methods for tracheal graft research have presented persistent challenges to investigators, and three-dimensional (3D)-printed biosynthetic grafts offer one potential development platform. We aimed to develop an efficient research platform for customizable circumferential 3D-printed tracheal grafts and evaluate feasibility and early structural integrity with a large-animal model. METHODS: Virtual 3D models of porcine subject tracheas were generated using preoperative computed tomography scans...
June 13, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28613055/-comparison-of-adaptation-and-microstructure-of-titanium-upper-complete-denture-base-fabricated-by-selecting-laser-melting-and-electron-beam-melting
#5
Y Ye, Y Y Xiong, J R Zhu, J Sun
Objective: To fabricate Ti alloy frameworks for a maxillary complete denture with three-dimensional printing (3DP) technique, such as selective laser melting (SLM) and electron beam melting (EBM), and to evaluate the microstructure of these frameworks and their adaptation to the die stone models. Methods: Thirty pairs of edentulous casts were divided into 3 groups randomly and equally. In each group, one of the three techniques (SLM, EBM, conventional technique) was used to fabricate Ti alloy frameworks. The base-cast sets were transversally sectioned into 3 sections at the distal of canines, mesial of first molars, and the posterior palatal zone...
June 9, 2017: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/28610885/three-dimensional-printed-bioengineered-tracheal-grafts-preclinical-results-and-potential-for-human-use
#6
Sadiq S Rehmani, Adnan M Al-Ayoubi, Adil Ayub, Michael Barsky, Erik Lewis, Raja Flores, Robert Lebovics, Faiz Y Bhora
BACKGROUND: We aimed to develop a process using three-dimensional (3D) printing to create bioengineered tracheal grafts (BETGs) for reconstruction of anterior tracheal defects in a large-animal model (porcine) that would have translational relevance for potential human use. METHODS: Preoperative computed tomographic scans were used to create virtual 3D models of the animal airways. Anatomically scaled tracheal grafts were subsequently developed using 3D-printed polycaprolactone and extracellular matrix...
June 11, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28598722/improvement-of-mechanical-strength-and-osteogenic-potential-of-calcium-sulfate-based-hydroxyapatite-3-dimensional-printed-scaffolds-by-%C3%AE%C2%B5-polycarbonate-coating
#7
Beom-Su Kim, Sun-Sik Yang, Ho Park, Se-Hwan Lee, Young-Sam Cho, Jun Lee
Powder-based three-dimensional (3D) printing is an excellent method to fabricate complex-shaped scaffolds for tissue engineering. However, their lower mechanical strength restricts their application in bone tissue engineering. Here, we created a 3D-printed scaffold coated with a ε-polycaprolactone (PCL) polymer solution (5 and 10 w/v %) to improve the mechanical strength of the scaffold. The 3D scaffold was fabricated from calcium sulfate hemihydrate powder (CaSO4-1/2 H2O), transformed into hydroxyapatite (HAp) by treatment with a hydrothermal reaction in an NH4H2PO4 solution...
June 21, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28594387/3d-printing-based-integrated-water-quality-sensing-system
#8
Muinul Banna, Kaustav Bera, Ryan Sochol, Liwei Lin, Homayoun Najjaran, Rehan Sadiq, Mina Hoorfar
The online and accurate monitoring of drinking water supply networks is critically in demand to rapidly detect the accidental or deliberate contamination of drinking water. At present, miniaturized water quality monitoring sensors developed in the laboratories are usually tested under ambient pressure and steady-state flow conditions; however, in Water Distribution Systems (WDS), both the pressure and the flowrate fluctuate. In this paper, an interface is designed and fabricated using additive manufacturing or 3D printing technology-material extrusion (Trade Name: fused deposition modeling, FDM) and material jetting-to provide a conduit for miniaturized sensors for continuous online water quality monitoring...
June 8, 2017: Sensors
https://www.readbyqxmd.com/read/28541035/three-dimensional-printing-of-silver-microarchitectures-using-newtonian-nanoparticle-inks
#9
Sanghyeon Lee, Jung Hyun Kim, Muhammad Wajahat, Hwakyung Jeong, Won Suk Chang, Sung Ho Cho, Ji Tae Kim, Seung Kwon Seol
Although three-dimensional (3D) printing has recently emerged as a technology to potentially bring about the next industrial revolution, the limited selection of usable materials restricts its use to simple prototyping. In particular, metallic 3D printing with submicrometer spatial resolution is essential for the realization of 3D-printed electronics. Herein, a meniscus-guided 3D printing method that exploits a low-viscosity (∼7 mPa·s) silver nanoparticle (AgNP) ink meniscus with Newtonian fluid characteristics (which is compatible with conventional inkjet printers) to fabricate 3D silver microarchitectures is reported...
June 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28488804/three-dimensional-shape-and-surface-features-distinguish-multiple-sclerosis-lesions-from-nonspecific-white-matter-disease
#10
Braeden D Newton, Katy Wright, Mandy D Winkler, Francesca Bovis, Masaya Takahashi, Ivan E Dimitrov, Maria Pia Sormani, Marco C Pinho, Darin T Okuda
BACKGROUND AND PURPOSE: There remains a need to further refine the ability of clinicians to differentiate multiple sclerosis (MS) from other disease etiologies. Here, we illustrate the value of 3-dimensional (3D) geometric shape and surface lesion characteristics between disease states. METHODS: Standardized 3-Tesla 3D brain magnetic resonance imaging studies were performed on enrolled MS and nonspecific white matter (NSWM) patients. Focal supratentorial lesions were identified, reconstructed using maximum intensity projection, manually segmented, and 3D printed...
May 10, 2017: Journal of Neuroimaging: Official Journal of the American Society of Neuroimaging
https://www.readbyqxmd.com/read/28471308/fabrication-of-orientation-controlled-3d-tissues-using-a-layer-by-layer-technique-and-3d-printed-a-thermoresponsive-gel-frame
#11
Yoshinari Tsukamoto, Takami Akagi, Fumiaki Shima, Mitsuru Akashi
Herein, we report the fabrication of orientation-controlled tissues similar to heart and nerve tissues using a cell accumulation and three-dimensional (3D) printing technique. We first evaluated the 3D shaping ability of hydroxybutyl chitosan (HBC), a thermoresponsive polymer, by using a robotic dispensing 3D printer. HBC polymer could be laminated to a height of 1124 ± 14 μm. Based on this result, we fabricated 3D gel frames of various shapes, such as square, triangular, rectangular, and circular, for shape control of 3D tissue and then normal human cardiac fibroblasts (NHCFs) coated with extracellular matrix nanofilms were seeded in the frames...
June 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28467835/self-supporting-nanoclay-as-internal-scaffold-material-for-direct-printing-of-soft-hydrogel-composite-structures-in-air
#12
Yifei Jin, Chengcheng Liu, Wenxuan Chai, Ashley Compaan, Yong Huang
Three dimensional (3D) bioprinting technology enables the freeform fabrication of complex constructs from various hydrogels and is receiving increasing attention in tissue engineering. The objective of this study is to develop a novel self-supporting direct hydrogel printing approach to extrude complex 3D hydrogel composite structures in air without the help of a support bath. Laponite, a member of the smectite mineral family, is investigated to serve as an internal scaffold material for the direct printing of hydrogel composite structures in air...
May 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28445375/a-new-classification-of-three-dimensional-printing-technologies-systematic-review-of-three-dimensional-printing-for-patient-specific-craniomaxillofacial-surgery
#13
Carly A Jacobs, Alexander Y Lin
BACKGROUND: Three-dimensional printing technology has been advancing in surgical applications. This systematic review examines its patient-specific applications in craniomaxillofacial surgery. METHODS: Terms related to "three-dimensional printing" and "surgery" were searched on PubMed on May 4, 2015; 313 unique articles were returned. Inclusion and exclusion criteria concentrated on patient-specific surgical applications, yielding 141 full-text articles, of which 33 craniomaxillofacial articles were analyzed...
May 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28440728/characterization-of-chemical-contaminants-generated-by-a-desktop-fused-deposition-modeling-3-dimensional-printer
#14
Aleksandr B Stefaniak, Ryan F LeBouf, Jinghai Yi, Jason Ham, Timothy Nurkewicz, Diane E Schwegler-Berry, Bean T Chen, J Raymond Wells, Matthew G Duling, Robert B Lawrence, Stephen B Martin, Alyson R Johnson, M Abbas Virji
Printing devices are known to emit chemicals into the indoor atmosphere. Understanding factors that influence release of chemical contaminants from printers is necessary to develop effective exposure assessment and control strategies. In this study, a desktop fused deposition modeling (FDM) 3-D printer using acrylonitrile butadiene styrene (ABS) or polylactic acid (PLA) filaments and two monochrome laser printers were evaluated in a 0.5 m(3) chamber. During printing, chamber air was monitored for vapors using a real-time photoionization detector (results expressed as isobutylene equivalents) to measure total volatile organic compound (TVOC) concentrations, evacuated canisters to identify specific VOCs by off-line gas chromatography-mass spectrometry (GC-MS) analysis, and liquid bubblers to identify carbonyl compounds by GC-MS...
April 25, 2017: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/28438549/poly-methyl-vinyl-ether-co-maleic-acid-pectin-based-hydrogel-forming-systems-gel-film-and-microneedles
#15
Yusuf Kemal Demir, Ayşegül Ülkü Metin, Betül Şatıroğlu, Mehmet Ertuğrul Solmaz, Veysel Kayser, Karsten Mäder
Cross-linking of natural and synthetic polymers is widely explored to achieve the desired material properties (mechanical strength, drug loading capacity, swelling and erosion rates). However, the potential of polymers produced by crosslinking poly (methyl vinyl ether-co-maleic acid) (PMVE/MA) and pectin (PE) in pharmaceutics is mainly unexplored so far. We have investigated the effect of various esterification conditions and pectin content on the physicochemical properties. Materials have been characterized by fourier transform infrared, differential scanning calorimetry and scanning electron microscopy...
April 21, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28431980/shear-stress-alterations-in-the-celiac-trunk-of-patients-with-a-continuous-flow-left-ventricular-assist-device-as-shown-by-in-silico-and-in-vitro-flow-analyses
#16
Francesco Scardulla, Salvatore Pasta, Leonardo D'Acquisto, Sergio Sciacca, Valentina Agnese, Christian Vergara, Alfio Quarteroni, Francesco Clemenza, Diego Bellavia, Michele Pilato
BACKGROUND: The use of left ventricular assist devices (LVADs) to treat advanced cardiac heart failure is constantly increasing, although this device leads to high risk for gastrointestinal bleeding. METHODS: Using in-silico flow analysis, we quantified hemodynamic alterations due to continuous-flow LVAD (HeartWare, Inc., Framingham, MA) in the celiac trunk and major branches of the abdominal aorta, and then explored the relationship between wall shear stress (WSS) and celiac trunk orientation...
March 24, 2017: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/28389072/the-characteristics-of-mineral-trioxide-aggregate-polycaprolactone-3-dimensional-scaffold-with-osteogenesis-properties-for-tissue-regeneration
#17
Yung-Cheng Chiu, Hsin-Yuan Fang, Tuan-Ti Hsu, Cheng-Yao Lin, Ming-You Shie
INTRODUCTION: The aim of this study was to investigate whether the mineral trioxide aggregate/polycaprolactone (MTA/PCL) hybrid 3-dimensional (3D) scaffold supplies a suitable microenvironment for the osteogenic differentiation of human dental pulp cells (hDPCs) and to further consider the effect of the MTA/PCL composite on the biological performance of hybrid scaffolds. METHODS: MTA was suspended in absolute alcohol and dropped slowly into PCL that was generated with the printable MTA-matrix...
April 4, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28388595/customized-knee-prosthesis-in-treatment-of-giant-cell-tumors-of-the-proximal-tibia-application-of-3-dimensional-printing-technology-in-surgical-design
#18
Wenbin Luo, Lanfeng Huang, He Liu, Wenrui Qu, Xin Zhao, Chenyu Wang, Chen Li, Tao Yu, Qing Han, Jincheng Wang, Yanguo Qin
BACKGROUND We explored the application of 3-dimensional (3D) printing technology in treating giant cell tumors (GCT) of the proximal tibia. A tibia block was designed and produced through 3D printing technology. We expected that this 3D-printed block would fill the bone defect after en-bloc resection. Importantly, the block, combined with a standard knee joint prosthesis, provided attachments for collateral ligaments of the knee, which can maintain knee stability. MATERIAL AND METHODS A computed tomography (CT) scan was taken of both knee joints in 4 patients with GCT of the proximal tibia...
April 7, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28383365/design-and-validation-of-a-three-dimensional-printed-flexible-canine-otoscopy-teaching-model
#19
Belle Marie D Nibblett, Mary Mauldin Pereira, Fortune Sithole, Paul A D Orchard, Eric B Bauman
INTRODUCTION: A teaching model was sought to improve canine otoscopy skill and reduce use of teaching dogs. METHODS: An otoscopy teaching model was printed in a flexible medium on a desktop three-dimensional printer from a magnetic resonance image of a canine external ear canal. The model was mounted in a polyvinyl dog mannequin. Validation of the teaching model was sought from student, faculty, and dog perspective. Student perception of prelaboratory training was assessed using a survey regarding their experience...
April 5, 2017: Simulation in Healthcare: Journal of the Society for Simulation in Healthcare
https://www.readbyqxmd.com/read/28375818/chondrocyte-attachment-proliferation-and-differentiation-on-three-dimensional-polycaprolactone-fumarate-scaffolds
#20
Eric R Wagner, Joshua Parry, Mahrokh Dadsetan, Dalibel Bravo, Scott M Riester, Andre J van Wijnen, Michael J Yaszemski, Sanjeev Kakar
Current treatment options for cartilage injuries are limited. The goals of this study are to create a biodegradable polymer scaffold with the capabilities of sustaining chondrocyte growth and proliferation, enable cell-to-cell communication and tissue regeneration through large pores, and assess the biological augmentation of the scaffold capabilities using platelet lysate (PL). We synthesized biodegradable polycaprolactone fumarate (PCLF) scaffolds to allow cell-cell communication through large interconnected pores...
March 31, 2017: Tissue Engineering. Part A
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