Pseudonoma

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Haynes, William C. (Northern Utilization Research and Development Division, Peoria, Ill.) and Lenora J. Rhodes. Comparative taxonomy of crystallogenic strains of Pseudomonas aeruginosa and Pseudomonas chlororaphis. J. Bacteriol. 84:1080-1084. 1962.-Only 11 of 39 strains received in the Agricultural Research Service Culture Collection under the designation Pseudonomas chlororaphis proved to be authentic; 28 were typical, pyocyanogenic strains of P. aeruginosa. The reason for this disproportionately high rate of misidentification apparently arises from an erroneous belief that the ability to produce green and yellow crystals of chlororaphin and oxychlororaphin is confined to P...

BACKGROUND: A prevalence study regarding hospital acquired infections and particularly surgical wound infections was performed from 17-4-1995 to 17-7-1995 in the Voghera hospital, a large one in Northern Italy. METHODS: The records of all subjects who have operated since at least 24 hours have been checked and the surgical wounds have been classified according to the guidelines of CDC (Atlanta). RESULTS: The prevalence rate of surgical wound infections was 13...

Malignant external otitis is a life-threatening infection occurring in aging diabetic and immunocompromised patients. The development of new antimicrobial and diagnostic aids has modified the therapy and prognosis of the disease. We describe our experience in 12 cases seen between 1982 and 1991, and review the diagnostic and therapeutic criteria during this lapse. Ten cases were males and 11 were diabetics. The most common symptoms were unilateral otalgia and otorrhea. All had edema of the external auditory channel and nine, proliferation of granulation tissue...

The mode of interaction of the ketocarboxyl-group-recognizing enzyme oxaloacetate decarboxylase (OXAD) from Pseudonomas sp., with purpose-designed (keto)-carboxyl-terminal biomimetic monochlorotriazinyl-dyes (BM) and parent dichlorotriazinyl-dye Vilmafix blue A-R (VBAR) was investigated. Kinetic inhibition studies and determinations of KD values of the respective dye-enzyme complex from both difference spectra and enzyme inactivation studies were employed. Substratemimetic (biomimetic) dye-ligands bear a terminal (keto)carboxyl-moiety linked to the reactive chlorotriazine ring, thus mimicking the organic acid substrate of OXAD...

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A Pseudomonas species grown on polyamines was found to have the following enzymes that can dehydrogenate succinic semialdehyde: a constitutive nicotinamide adenine dinucleotide phosphate (NADP)-linked dehydrogenase, an inducible NAD-linked dehydrogenase specific for succinic semialdehyde (EC 1.2.lb), and more than one inducible NAD-linked aminoaldehyde dehydrogenase which can act on succinic semialdehyde, 3-aminopropanal, and 4-aminobutanal. These enzymes have been separated from each other by ammonium sulfate precipitation, column chromatography on diethylaminoethyl-Sephadex, and electrophoresis on polyacrylamide gel...

Nineteen soils, three freshwater lake sediments, and oxidized poultry manure were examined to determine the dominant denitrifier populations. The samples, most shown or expected to support active denitrification, were from eight countries and included rice paddy, temperate agricultural, rain forest, organic, and waste-treated soils. Over 1,500 organisms that could grow anaerobically on nitrate agar were isolated. After purification, 146 denitrifiers were obtained, as verified by production of N(2) from NO(3) (-)...

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The M.B.C.'s of gentamicin and carbenicillin against Pseudonomas aeruginosa NCTC 10490 were measured under controlled conditions using a Biophotometer. The M.B.C. of gentamicin was 15 mug/ml but even in a concentration of 1,000 mug/ml carbenicillin was not bactericidal. In further experiments, subinhibitory concentrations of gentamicin (1 mug/ml) together with varying concentrations of carbenicillin were added to a log phase culture of the organism. Under these conditions the M.B.C. of carbenicillin was now 6 mug/ml...

Lipopolysaccharide antigens were extracted from heated cell extracts of several serotypes of Pseudonomas aeruginosa. Indirect hemagglutination with the extracts indicated specific reactivity with sera from rabbits immunized with homologous serotypes of P. aeruginosa. Sera from healthy adults and patients infected with P. aeruginosa were studied subsequently and shown to possess antibodies against P. aeurginosa. In patients infected with P. aeruginosa type E, indirect hemagglutination antibody against type E was resistant to 2-mercaptoethanol (2-ME) and classified as immunoglobulin G...

The pyruvate carboxylase of Pseudonomas fluorescens was purified 160-fold from cells grown on glucose at 20 degrees C. The activity of this purified enzyme was not affected by acetyl-coenzyme A or L-aspartate, but was strongly inhibited by ADP, which was competitive towards ATP. Pyruvate gave a broken double reciprocal plot, from which two apparent Km values could be determined, namely 0-08 and 0-21 mM, from the lower and the higher concentration ranges, respectively. The apparent Km for HCO3 at pH 6-9, in the presence of the manganese ATP ion (MnATP2-), was 3-1 mM...