keyword
MENU ▼
Read by QxMD icon Read
search

Blood type lectin

keyword
https://www.readbyqxmd.com/read/28988747/structural-insights-into-vlr-fine-specificity-for-blood-group-carbohydrates
#1
Bernard C Collins, Robin J Gunn, Tanya R McKitrick, Richard D Cummings, Max D Cooper, Brantley R Herrin, Ian A Wilson
High-quality reagents to study and detect glycans with high specificity for research and clinical applications are severely lacking. Here, we structurally and functionally characterize several variable lymphocyte receptor (VLR)-based antibodies from lampreys immunized with O erythrocytes that specifically recognize the blood group H-trisaccharide type II antigen. Glycan microarray analysis and biophysical data reveal that these VLRs exhibit greater specificity for H-trisaccharide compared with the plant lectin UEA-1, which is widely used in blood typing...
September 27, 2017: Structure
https://www.readbyqxmd.com/read/28980000/increased-indoleamine-2-3-dioxygenase-and-quinolinic-acid-expression-in-microglia-and-m%C3%A3-ller-cells-of-diabetic-human-and-rodent-retina
#2
Ping Hu, Nicholas H Hunt, Frank Arfuso, Lynn C Shaw, Mohammad Nasir Uddin, Meidong Zhu, Raj Devasahayam, Samuel J Adamson, Vicky L Benson, Tailoi Chan-Ling, Maria B Grant
Purpose: We investigated the relationship between inflammation, neuronal loss, and expression of indoleamine 2, 3-dioxygenase (IDO) and quinolinic acid (QUIN) in the retina of subjects with type 1 diabetes (T1D) and type 2 diabetes (T2D) and in the retina of rats with T1D. Methods: Retinas from T1D (n = 7), T2D (n = 13), and 20 age-matched nondiabetic human donors and from T1D (n = 3) and control rats (n = 3) were examined using immunohistochemistry for IDO, QUIN, cluster of differentiation 39 (CD39), ionized calcium-binding adaptor molecule (Iba-1, for macrophages and microglia), Vimentin (VIM; for Müller cells), neuronal nuclei (NeuN; for neurons), and UEA1 lectin (for blood vessels)...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28970495/molecular-basis-of-human-cd22-function-and-therapeutic-targeting
#3
June Ereño-Orbea, Taylor Sicard, Hong Cui, Mohammad T Mazhab-Jafari, Samir Benlekbir, Alba Guarné, John L Rubinstein, Jean-Philippe Julien
CD22 maintains a baseline level of B-cell inhibition to keep humoral immunity in check. As a B-cell-restricted antigen, CD22 is targeted in therapies against dysregulated B cells that cause autoimmune diseases and blood cancers. Here we report the crystal structure of human CD22 at 2.1 Å resolution, which reveals that specificity for α2-6 sialic acid ligands is dictated by a pre-formed β-hairpin as a unique mode of recognition across sialic acid-binding immunoglobulin-type lectins. The CD22 ectodomain adopts an extended conformation that facilitates concomitant CD22 nanocluster formation on B cells and binding to trans ligands to avert autoimmunity in mammals...
October 2, 2017: Nature Communications
https://www.readbyqxmd.com/read/28905193/siglec-8-as-mast-cell-selective-target-developing-paradigms-amidst-inconvenient-truths
#4
Gerhard J Molderings
Due to the limited efficacy of current drugs in treating systemic mast cell activation disease, there is an urgent need for more effective drugs selectively acting at mast cells. In the past, a large number of compounds have been claimed to be effective and mast cell selective on the basis of cell culture experiments and studies on blood leukocytes which could not be verified in organ and animal studies. Nevertheless, over time in review papers about potential mast cell targets mast cell selectivity of these targets has been no longer challenged...
September 14, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28867209/identification-and-molecular-profiling-of-dc-sign-like-from-big-belly-seahorse-hippocampus-abdominalis-inferring-its-potential-relevancy-in-host-immunity
#5
Eunyoung Jo, Don Anushka Sandaruwan Elvitigala, Qiang Wan, Minyoung Oh, Chulhong Oh, Jehee Lee
Dendritic-cell-specific ICAM-3-grabbing non-integrin (DC-SIGN) is a C-type lectin that functions as a pattern recognition receptor by recognizing pathogen-associated molecular patterns (PAMPs). It is also involved in various events of the dendritic cell (DC) life cycle, such as DC migration, antigen capture and presentation, and T cell priming. In this study, a DC-SIGN-like gene from the big belly seahorse Hippocampus abdominalis (designated as ShDCS-like) was identified and molecularly characterized. The putative, complete ORF was found to be 1368 bp in length, encoding a protein of 462 amino acids with a molecular mass of 52...
December 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28865488/distinct-transcriptional-and-metabolic-profiles-associated-with-empathy-in-buddhist-priests-a-pilot-study
#6
Junji Ohnishi, Satoshi Ayuzawa, Seiji Nakamura, Shigeko Sakamoto, Miyo Hori, Tomoko Sasaoka, Eriko Takimoto-Ohnishi, Masakazu Tanatsugu, Kazuo Murakami
BACKGROUND: Growing evidence suggests that spiritual/religious involvement may have beneficial effects on both psychological and physical functions. However, the biological basis for this relationship remains unclear. This study explored the role of spiritual/religious involvement across a wide range of biological markers, including transcripts and metabolites, associated with the psychological aspects of empathy in Buddhist priests. METHODS: Ten professional Buddhist priests and 10 age-matched non-priest controls were recruited...
September 2, 2017: Human Genomics
https://www.readbyqxmd.com/read/28860481/increased-expression-of-siglec-9-in-chronic-obstructive-pulmonary-disease
#7
Zhilin Zeng, Miao Li, Meijia Wang, Xiaomei Wu, Qinghai Li, Qin Ning, Jianping Zhao, Yongjian Xu, Jungang Xie
Chronic obstructive pulmonary disease (COPD) is a common inflammatory lung disease. Sialic acid-binding immunoglobulin-type lectins 9 (Siglec-9) is predominantly expressed on innate immune cells and has been shown to exert regulatory effect on immune cells through glycan recognition. Soluble Siglec-9 (sSiglec-9), the extracellular region of Siglec-9, might fulfill its function partly by competitive inhibiting siglec-9 binding to its ligands; however, the role of Siglec-9 and sSiglec-9 in the pathogenesis COPD remain largely unknown...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28858976/galectin-3-pharmacological-inhibition-attenuates-early-renal-damage-in-spontaneously-hypertensive-rats
#8
Ernesto Martínez-Martínez, Jaime Ibarrola, Amaya Fernández-Celis, Laurent Calvier, Celine Leroy, Victoria Cachofeiro, Patrick Rossignol, Natalia López-Andrés
BACKGROUND: The pharmacological blockade of galectin-3 (Gal-3), a β-galactoside-binding lectin, reduces renal impairment in acute kidney injury, hyperaldosteronism or nephropathy. We herein investigated the effects of pharmacological Gal-3 inhibition by modified citrus pectin (MCP) in renal damage in spontaneously hypertensive rats (SHRs). METHODS AND RESULTS: Gal-3 inhibition did not modify blood pressure levels in 30-week-old SHR. Kidney weight was higher in SHR, with no effect of MCP treatment (100 mg/kg/day in the drinking water)...
August 30, 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28858830/exposure-to-low-levels-of-lead-in-utero-and-umbilical-cord-blood-dna-methylation-in-project-viva-an-epigenome-wide-association-study
#9
Shaowei Wu, Marie-France Hivert, Andres Cardenas, Jia Zhong, Sheryl L Rifas-Shiman, Golareh Agha, Elena Colicino, Allan C Just, Chitra Amarasiriwardena, Xihong Lin, Augusto A Litonjua, Dawn L DeMeo, Matthew W Gillman, Robert O Wright, Emily Oken, Andrea A Baccarelli
BACKGROUND: Early-life exposure to lead is associated with deficits in neurodevelopment and with hematopoietic system toxicity. DNA methylation may be one of the underlying mechanisms for the adverse effects of prenatal lead on the offspring, but epigenome-wide methylation data for low levels of prenatal lead exposure are lacking. OBJECTIVES: We investigated the association between prenatal maternal lead exposure and epigenome-wide DNA methylation in umbilical cord blood nucleated cells in Project Viva, a prospective U...
August 25, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28854748/pattern-recognition-receptor-genes-expression-profiling-in-indigenous-chickens-of-india-and-white-leghorn
#10
S Haunshi, Arun Kumar Burramsetty, T R Kannaki, K S Raja Ravindra, R N Chatterjee
Pattern recognition receptors (PRR) such as Toll-like receptors, NOD-like receptors, RIG-I helicase receptors, and C-type lectin receptors play a critical role in innate immunity as a first line of defense against invading pathogens through recognition of pathogen and/or damage-associated molecular patterns. Genetic makeup of birds is known to play a role in resistance or susceptibility to various infectious diseases. Therefore, the present study was carried out to elucidate the differential expression of PRR and some of the cytokine genes in peripheral blood mononuclear cells of indigenous chicken breeds such as Ghagus and Nicobari and an exotic chicken breed, White Leghorn (WLH)...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28836308/siglec-h-is-a-microglia-specific-marker-that-discriminates-microglia-from-cns-associated-macrophages-and-cns-infiltrating-monocytes
#11
Hiroyuki Konishi, Masaaki Kobayashi, Taikan Kunisawa, Kenta Imai, Akira Sayo, Bernard Malissen, Paul R Crocker, Katsuaki Sato, Hiroshi Kiyama
Several types of myeloid cell are resident in the CNS. In the steady state, microglia are present in the CNS parenchyma, whereas macrophages reside in boundary regions of the CNS, such as perivascular spaces, the meninges and choroid plexus. In addition, monocytes infiltrate into the CNS parenchyma from circulation upon blood-brain barrier breakdown after CNS injury and inflammation. Although several markers, such as CD11b and ionized calcium-binding adapter molecule 1 (Iba1), are frequently used as microglial markers, they are also expressed by other types of myeloid cell and microglia-specific markers were not defined until recently...
August 24, 2017: Glia
https://www.readbyqxmd.com/read/28835437/clec-2-contributes-to-hemostasis-independently-of-classical-hemitam-signaling-in-mice
#12
Elizabeth J Haining, Deya Cherpokova, Karen Wolf, Isabelle C Becker, Sarah Beck, Johannes A Eble, David Stegner, Steve P Watson, Bernhard Nieswandt
C-type lectin-like receptor 2 (CLEC 2) is a platelet receptor that is critical during development in blood-lymph separation and implicated in thrombus stability in thrombosis and hemostasis. It is the only known platelet activatory receptor that participates in both these aspects of platelet function and is the only one to signal through a hemi-immunoreceptor tyrosine-based activation motif (hemITAM). Current investigations into the function of CLEC 2 in vivo have focused on knock-out studies in which both the receptor and its signaling are deleted, making it impossible to explore the possible signaling independent functions of the receptor which are indicated by its only known physiological ligand, podoplanin, being an integral membrane protein...
August 23, 2017: Blood
https://www.readbyqxmd.com/read/28806750/characterization-of-novel-bangle-lectin-from-photorhabdus-asymbiotica-with-dual-sugar-binding-specificity-and-its-effect-on-host-immunity
#13
Gita Jančaříková, Josef Houser, Pavel Dobeš, Gabriel Demo, Pavel Hyršl, Michaela Wimmerová
Photorhabdus asymbiotica is one of the three recognized species of the Photorhabdus genus, which consists of gram-negative bioluminescent bacteria belonging to the family Morganellaceae. These bacteria live in a symbiotic relationship with nematodes from the genus Heterorhabditis, together forming a complex that is highly pathogenic for insects. Unlike other Photorhabdus species, which are strictly entomopathogenic, P. asymbiotica is unique in its ability to act as an emerging human pathogen. Analysis of the P...
August 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28764923/association-of-rs1285933-single-nucleotide-polymorphism-in-clec5a-gene-with-dengue-severity-and-its-functional-effects
#14
Caroline Xavier-Carvalho, Renata Duarte da Silva Cezar, Naishe Matos Freire, Carla Mola, Victor Fiesta, Thiago Gomes de Toledo-Pinto, Luciana Gomes Fialho Torres, Rodrigo Feliciano do Carmo, Luydson Richardson Silva Vasconcelos, Marli Tenório Cordeiro, Paulo Baptista, Elzinandes Leal de Azeredo, Rivaldo Venâncio da Cunha, Luiz José de Souza, Antonio Guilherme Pacheco, Claire Fernandes Kubelka, Patrícia Moura, Milton Ozorio Moraes
Outbreaks of the Zika, dengue, and chikungunya viruses, especially in the Americas, pose a global threat due to their rapid spread and difficulty controlling the vector. Extreme phenotypes are often observed, from asymptomatic to severe clinical manifestations, which are well-studied in dengue. Host variations are also important contributors to disease outcomes, and many case-control studies have associated single nucleotide polymorphisms (SNPs) with severe dengue. Here, we found that the TC genotype and T-carriers for SNP rs1285933 in the C-type lectin superfamily member 5 (CLEC5A) gene was associated with severe dengue in a Northern Brazilian population (OR=2...
July 29, 2017: Human Immunology
https://www.readbyqxmd.com/read/28751823/global-autorecognition-and-activation-of-complement-by-mannan-binding-lectin-in-a-mouse-model-of-type-1-diabetes
#15
Esben Axelgaard, Jakob Appel Østergaard, Saranda Haxha, Steffen Thiel, Troels Krarup Hansen
Increasing evidence links mannan-binding lectin (MBL) to late vascular complications of diabetes. MBL is a complement-activating pattern recognition molecule of the innate immune system that can mediate an inflammation response through activation of the lectin pathway. In two recent animal studies, we have shown that autoreactivity of MBL is increased in the kidney in diabetic nephropathy. We hypothesize that long-term exposure to uncontrolled high blood glucose in diabetes may mediate formation of neoepitopes in several tissues and that MBL is able to recognize these structures and thus activate the lectin pathway...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28751211/microbial-f-type-lectin-domains-with-affinity-for-blood-group-antigens
#16
Sonal Mahajan, Aasawari Khairnar, Ritika Bishnoi, T N C Ramya
F-type lectins are fucose binding lectins with characteristic fucose binding and calcium binding motifs. Although they occur with a selective distribution in viruses, prokaryotes and eukaryotes, most biochemical studies have focused on vertebrate F-type lectins. Recently, using sensitive bioinformatics search techniques on the non-redundant database, we had identified many microbial F-type lectin domains with diverse domain organizations. We report here the biochemical characterization of F-type lectin domains from Cyanobium sp...
September 23, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28734845/leveraging-siglec-8-endocytic-mechanisms-to-kill-human-eosinophils-and-malignant-mast-cells
#17
Jeremy A O'Sullivan, Daniela J Carroll, Yun Cao, Adriano N Salicru, Bruce S Bochner
BACKGROUND: Sialic acid-binding immunoglobulin-like lectin (Siglec)-8 is a cell-surface protein expressed selectively on human eosinophils, mast cells, and basophils, making it an ideal target for the treatment of diseases involving these cell types. However, the effective delivery of therapeutic agents to these cells requires an understanding of the dynamics of Siglec-8 surface expression. OBJECTIVES: We sought to determine whether Siglec-8 is endocytosed in human eosinophils and malignant mast cells, identify mechanisms underlying its endocytosis, and demonstrate whether a toxin can be targeted to Siglec-8-bearing cells to kill these cells...
July 20, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28718199/the-clec12a-receptor-marks-human-basophils-potential-implications-for-minimal-residual-disease-detection-in-acute-myeloid-leukemia
#18
Marie Toft-Petersen, Anne Stidsholt Roug, Trine Plesner, Lene Ebbesen, Gordon D Brown, Line Nederby
BACKGROUND: The transmembrane receptor C-type lectin domain family 12, member A (CLEC12A) is known to be highly expressed on monocytes and neutrophils and is a reliable leukemia associated marker in acute myeloid leukemia. Consequently, detailed knowledge of the various normal cell types expressing this receptor is essential. We have observed CLEC12A to be expressed on CD45lowSSClowCD14-CD123+ basophils in peripheral blood (PB) and in this study, we aimed at verifying this observation and further delineate the CD45lowSSClowCD14-CD123 + CLEC12A+ subpopulation...
July 17, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28715655/macrophage-exosomes-as-natural-nanocarriers-for-protein-delivery-to-inflamed-brain
#19
Dongfen Yuan, Yuling Zhao, William A Banks, Kristin M Bullock, Matthew Haney, Elena Batrakova, Alexander V Kabanov
Recent work has stimulated interest in the use of exosomes as nanocarriers for delivery of small drugs, RNAs, and proteins to the central nervous system (CNS). To overcome the blood-brain barrier (BBB), exosomes were modified with brain homing peptides that target brain endothelium but likely to increase immune response. Here for the first time we demonstrate that there is no need for such modification to penetrate the BBB in mammals. The naïve macrophage (Mϕ) exosomes can utilize, 1) on the one hand, the integrin lymphocyte function-associated antigen 1 (LFA-1) and intercellular adhesion molecule 1 (ICAM-1), and, 2) on the other hand, the carbohydrate-binding C-type lectin receptors, to interact with brain microvessel endothelial cells comprising the BBB...
October 2017: Biomaterials
https://www.readbyqxmd.com/read/28713010/hemolectin-expression-reveals-functional-heterogeneity-in-honey-bee-apis-mellifera-hemocytes
#20
Erika Gábor, Gyöngyi Cinege, Gábor Csordás, Tibor Török, Katalin Folkl-Medzihradszky, Zsuzsanna Darula, István Andó, Éva Kurucz
The identification of molecular markers considerably facilitated the classification and functional analysis of blood cell types. Apis mellifera hemocytes have been classified by morphological criteria and lectin binding properties; however, the use of molecular markers has been minimal. Here we describe a monoclonal antibody to a non-phagocytic subpopulation of A. mellifera hemocytes and to a constituent of the hemolymph clot. We demonstrate that the antibody identifies the A. mellifera hemolectin, a protein carrying human von Willebrand factor homology domains, characteristic of proteins involved in blood coagulation and platelet aggregation in mammals...
July 13, 2017: Developmental and Comparative Immunology
keyword
keyword
15233
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"