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Blood type lectin

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https://www.readbyqxmd.com/read/29447367/endometrial-natural-killer-nk-cells-reveal-a-tissue-specific-receptor-repertoire
#1
D Feyaerts, T Kuret, B van Cranenbroek, S van der Zeeuw-Hingrez, O W H van der Heijden, A van der Meer, I Joosten, R G van der Molen
STUDY QUESTION: Is the natural killer (NK) cell receptor repertoire of endometrial NK (eNK) cells tissue-specific? SUMMARY ANSWER: The NK cell receptor (NKR) expression profile in pre-pregnancy endometrium appears to have a unique tissue-specific phenotype, different from that found in NK cells in peripheral blood, suggesting that these cells are finely tuned towards the reception of an allogeneic fetus. WHAT IS KNOWN ALREADY: NK cells are important for successful pregnancy...
February 13, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29427319/distinguishing-myelofibrosis-from-polycythemia-vera-and-essential-thrombocythemia-the-utility-of-enumerating-circulating-stem-cells-with-aberrant-hmicl-expression-by-flow-cytometry
#2
L L Herborg, L Nederby, H C Hasselbalch, A Aggerholm, A S Roug
INTRODUCTION: Diagnosing BCR-ABL negative myeloproliferative neoplasms (MPN) may be challenging due to overlapping features and lack of robust discriminatory parameters, especially between essential thrombocythemia (ET) and prefibrotic myelofibrosis (MF). Circulating immature hematopoietic cells are variably present in polycythemia vera (PV), ET, and MF. The C-type lectin hMICL is aberrantly expressed on hematopoietic stem cells in the majority of acute myeloid leukemia patients. However, the hMICL expression in MPN, having varying propensity of leukemic transformation, is unsettled...
February 10, 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29384203/improving-the-recombinant-human-erythropoietin-glycosylation-through-microsomes-in-cho-cell-free-system
#3
Chandrasekhar Gurramkonda, Aniruddha Rao, Shayan Borhani, Manohar Pilli, Sevda Deldari, Xudong Ge, Niloufar Pezeshk, Tzu-Chiang Han, Michael Tolosa, Yordan Kostov, Leah Tolosa, David W Wood, Krishna Vattem, Douglas D Frey, Govind Rao
Cell-Free Protein Synthesis (CFPS) offers many advantages for the production of recombinant therapeutic proteins using the CHO cell-free system. However, many complex proteins are still difficult to express using this method. To investigate the current bottlenecks in cell-free glycoprotein production, we chose erythropoietin (40% glycosylated), an essential endogenous hormone which stimulates the development of red blood cells. Here, we report the production of recombinant erythropoietin (EPO) using CHO cell-free system...
January 31, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29373511/poly-n-acetyllactosamine-neo-glycoproteins-as-nanomolar-ligands-of-human-galectin-3-binding-kinetics-and-modeling
#4
Ladislav Bumba, Dominic Laaf, Vojtěch Spiwok, Lothar Elling, Vladimír Křen, Pavla Bojarová
Galectin-3 (Gal-3) is recognized as a prognostic marker in several cancer types. Its involvement in tumor development and proliferation makes this lectin a promising target for early cancer diagnosis and anti-cancer therapies. Gal-3 recognizes poly-N-acetyllactosamine (LacNAc)-based carbohydrate motifs of glycoproteins and glycolipids with a high specificity for internal LacNAc epitopes. This study analyzes the mode and kinetics of binding of Gal-3 to a series of multivalent neo-glycoproteins presenting complex poly-LacNAc-based oligosaccharide ligands on a scaffold of bovine serum albumin...
January 26, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29361560/transient-loss-of-venous-integrity-during-developmental-vascular-remodeling-leads-to-red-blood-cell-extravasation-and-clearance-by-lymphatic-vessels
#5
Yang Zhang, Nina Daubel, Simon Stritt, Taija Mäkinen
Maintenance of blood vessel integrity is critical for vascular homeostasis and mainly controlled at the level of endothelial cell (EC) junctions. Regulation of endothelial integrity has largely been investigated in the mature quiescent vasculature. Less is known how integrity is maintained during vascular growth and remodeling involving extensive junctional reorganization. Here we show that embryonic mesenteric blood vascular remodeling is associated with a transient loss of venous integrity and concomitant extravasation of red blood cells (RBC), followed by their clearance by the developing lymphatic vessels...
January 22, 2018: Development
https://www.readbyqxmd.com/read/29316944/car-t-cells-targeting-cll-1-as-an-approach-to-treat-acute-myeloid-leukemia
#6
Jinghua Wang, Siyu Chen, Wei Xiao, Wende Li, Liang Wang, Shuo Yang, Weida Wang, Liping Xu, Shuangye Liao, Wenjian Liu, Yang Wang, Nawei Liu, Jianeng Zhang, Xiaojun Xia, Tiebang Kang, Gong Chen, Xiuyu Cai, Han Yang, Xing Zhang, Yue Lu, Penghui Zhou
BACKGROUND: Acute myeloid leukemia (AML) is one of the most common types of adult acute leukemia. Standard chemotherapies can induce complete remission in selected patients; however, a majority of patients eventually relapse and succumb to the disease. Thus, the development of novel therapeutics for AML is urgently needed. Human C-type lectin-like molecule-1 (CLL-1) is a type II transmembrane glycoprotein, and its expression is restricted to myeloid cells and the majority of AML blasts...
January 10, 2018: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/29296975/cell-surface-c-type-lectin-like-receptor-clec-1-dampens-dendritic-cell-activation-and-downstream-th17-responses
#7
Maria Dolores Lopez Robles, Annaick Pallier, Virginie Huchet, Laetitia Le Texier, Severine Remy, Cecile Braudeau, Laurence Delbos, Aurelie Moreau, Cedric Louvet, Carole Brosseau, Pierre-Joseph Royer, Antoine Magnan, Franck Halary, Regis Josien, Maria-Cristina Cuturi, Ignacio Anegon, Elise Chiffoleau
Dendritic cells (DCs) represent essential antigen-presenting cells that are critical for linking innate and adaptive immunity, and influencing T-cell responses. Among pattern recognition receptors, DCs express C-type lectin receptors triggered by both exogenous and endogenous ligands, therefore dictating pathogen response, and also shaping T-cell immunity. We previously described in rat, the expression of the orphan C-type lectin-like receptor-1 (CLEC-1) by DCs and demonstrated in vitro its inhibitory role in downstream T helper 17 (Th17) activation...
March 28, 2017: Blood Advances
https://www.readbyqxmd.com/read/29288201/murine-red-blood-cells-lack-ligands-for-b-cell-siglecs-allowing-strong-activation-by-erythrocyte-surface-antigens
#8
Fernando Spiller, Corwin M Nycholat, Chika Kikuchi, James C Paulson, Matthew S Macauley
CD22 and sialic acid-binding Ig-like lectin (Siglec)-G are members of the Siglec family of inhibitory coreceptors expressed on B cells that participate in enforcement of peripheral B cell tolerance. We have shown previously that when a BCR engages its cognate Ag on a cell surface that also expresses Siglec ligands, B cell Siglecs are recruited to the immunological synapse, resulting in suppression of BCR signaling and B cell apoptosis. Because all cells display sialic acids, and CD22 and Siglec-G have distinct, yet overlapping, specificities for sialic acid-containing glycan ligands, any cell could, in principle, invoke this tolerogenic mechanism for cell surface Ags...
December 29, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29273833/prospective-observational-study-on-the-association-between-serum-mannose-binding-lectin-levels-and-severe-outcome-in-critically-ill-patients-with-pandemic-influenza-type-a-h1n1-infection
#9
Elie Zogheib, Remy Nyga, Marjorie Cornu, Boualem Sendid, Julien Monconduit, Vincent Jounieaux, Julien Maizel, Christine Segard, Taïeb Chouaki, Hervé Dupont
BACKGROUND: Mannose-binding lectin (MBL) plays an important role in the innate immune response. In addition to activating the complement, MBL can induce cytokine production and contribute to a deleterious inflammatory response with severe A(H1N1)pdm09 virus infection. Our aim was to determine if serum MBL levels correlate with the risk of mortality in intensive care units (ICU) patients with A(H1N1)pdm09 infection. METHODS: Prospective observational study was performed in ICU patients with acute respiratory distress syndrome due to influenza A(H1N1)pdm09 virus...
December 22, 2017: Lung
https://www.readbyqxmd.com/read/29210476/delayed-revascularization-of-islets-after-transplantation-by-il-6-blockade-in-pig-to-non-human-primate-islet-xenotransplantation-model
#10
Byoung-Hoon Min, Jun-Seop Shin, Jong-Min Kim, Seong-Jun Kang, Hyun-Je Kim, Il-Hee Yoon, Su-Kyoung Park, Ji-Won Choi, Min-Suk Lee, Chung-Gyu Park
BACKGROUND: Pancreatic islet transplantation is currently proven as a promising treatment for type 1 diabetes patients with labile glycemic control and severe hypoglycemia unawareness. Upon islet transplantation, revascularization is essential for proper functioning of the transplanted islets. As IL-6 is important for endothelial cell survival and systemic inflammation related to xenograft, the effect of IL-6 receptor antagonist, tocilizumab, on revascularization of the transplanted islets was examined in pig to non-human primate islet xenotransplantation model...
December 6, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29204053/induction-of-precocious-intestinal-maturation-in-t-cell-deficient-athymic-neonatal-rats
#11
Ester Arévalo Sureda, Catherine Gidlund, Björn Weström, Olena Prykhodko
AIM: To investigate whether gut maturation could be induced precociously in an athymic T-cell deficient neonatal rat model. METHODS: Fourteen day-old athymic (nude) rats (NIH-Foxn1rnu) were gavaged with either phytohaemagglutinin - a lectin from red kidney beans (PHA); trypsin - a protease (Prot); or water - vehicle (control) as a single dose on one day or once a day for 3-day. The nude rats were either nurtured by their mothers or cross-fostered by conventional foster dams of the Sprague-Dawley strain from days 3-5 after birth...
November 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29198892/rtb-lectin-mediated-delivery-of-lysosomal-%C3%AE-l-iduronidase-mitigates-disease-manifestations-systemically-including-the-central-nervous-system
#12
Li Ou, Michael J Przybilla, Brenda Koniar, Chester B Whitley
Mucopolysaccharidosis type I (MPS I) is a lysosomal disease resulting from deficiency in the α-L-iduronidase (IDUA) hydrolase and subsequent accumulation of glycosaminoglycan (GAG). Clinically, enzyme replacement therapy (ERT) with IDUA achieves negligible neurological benefits presumably due to blood-brain-barrier (BBB) limitations. To investigate the plant lectin ricin B chain (RTB) as a novel carrier for enzyme delivery to the brain, an IDUA:RTB fusion protein (IDUAL), produced in N. benthamiana leaves, was tested in a murine model of Hurler syndrome (MPS I)...
November 28, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/29187378/platelet-glycoprotein-vi-aids-in-local-immunity-during-gram-negative-pneumonia-derived-sepsis
#13
Theodora A M Claushuis, Alex F de Vos, Bernhard Nieswandt, Louis Boon, Joris J T H Roelofs, Onno J de Boer, Cornelis J van 't Veer, Tom van der Poll
Platelet collagen receptor glycoprotein(GP)VI and podoplanin receptor C-type lectin-like receptor (CLEC-)2 are receptors implicated in platelet activation that both signal via an immunoreceptor-tyrosine-based activation motif. Platelets are necessary for host defense and prevention of hemorrhage during sepsis, but the role of platelet GPVI and CLEC2 herein is unknown. To investigate this we infected mice depleted of platelet GPVI or CLEC2 by antibody treatment or GPVI-/- mice with the common human sepsis pathogen Klebsiella pneumoniae via the airways to induce pneumonia derived sepsis...
November 29, 2017: Blood
https://www.readbyqxmd.com/read/29154855/identification-and-characterization-of-a-calcium-dependent-lily-type-lectin-from-black-rockfish-sebastes-schlegelii-molecular-antennas-are-involved-in-host-defense-via-pathogen-recognition
#14
Roopasingam Kugapreethan, Qiang Wan, Jehanathan Nilojan, Jehee Lee
Lily-type lectins (LTLs) are soluble pathogen recognition receptors (PRRs) that contain one or more characteristic carbohydrate recognition domains (CRDs), through which LTLs bind reversibly and specifically to cognate sugar moieties present on the invading pathogen membrane and trigger the host innate immune responses. In this study, we identified a LTL homolog (SsLTL) from black rockfish (Sebastes schlegelii) transcriptome database and its functional roles in innate immunity was investigated in vitro and in vivo...
November 14, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29101228/mass-spectrometric-revival-of-an-l-rhamnose-and-d-galactose-specific-lectin-from-a-lost-strain-of-streptomyces
#15
Yoko Fujita-Yamaguchi, Karine Bagramyan, Yoshiki Yamaguchi, Akemi Ikeda, Naoshi Dohmae, Teresa B Hong, Markus Kalkum
Blood type B-specific Streptomyces S27S5 hemagglutinin (SHA) was discovered and characterized in the 1970s. Although strain 27S5 has been lost, the purified SHA protein survived intact under frozen conditions and retained its activity. Using modern techniques, here we further characterized SHA. Fourier-transform ion cyclotron resonance MS analysis determined SHA's average molecular mass as 13,314.67 Da. MS of digested SHA peptides, Streptomyces genomic database matching, and N-terminal sequencing solved the 131-residue amino acid sequence of SHA...
November 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29089413/transcriptomes-of-major-renal-collecting-duct-cell-types-in-mouse-identified-by-single-cell-rna-seq
#16
Lihe Chen, Jae Wook Lee, Chung-Lin Chou, Anil V Nair, Maria A Battistone, Teodor G Păunescu, Maria Merkulova, Sylvie Breton, Jill W Verlander, Susan M Wall, Dennis Brown, Maurice B Burg, Mark A Knepper
Prior RNA sequencing (RNA-seq) studies have identified complete transcriptomes for most renal epithelial cell types. The exceptions are the cell types that make up the renal collecting duct, namely intercalated cells (ICs) and principal cells (PCs), which account for only a small fraction of the kidney mass, but play critical physiological roles in the regulation of blood pressure, extracellular fluid volume, and extracellular fluid composition. To enrich these cell types, we used FACS that employed well-established lectin cell surface markers for PCs and type B ICs, as well as a newly identified cell surface marker for type A ICs, c-Kit...
November 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29078142/electronegative-l5-ldl-induces-the-production-of-g-csf-and-gm-csf-in-human-macrophages-through-lox-1-involving-nf-%C3%AE%C2%BAb-and-erk2-activation
#17
Tzu-Ching Yang, Po-Yuan Chang, Tzu-Ling Kuo, Shao-Chun Lu
BACKGROUND AND AIMS: Circulating levels of granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF) are associated with the severity of acute myocardial infarction (AMI). However, what causes increases in G-CSF and GM-CSF is unclear. In this study, we investigated whether L5-low-density lipoprotein (LDL), a mildly oxidized LDL from AMI, can induce G-CSF and GM-CSF production in human macrophages. METHODS: L1-LDL and L5-LDL were isolated through anion-exchange chromatography from AMI plasma...
October 14, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29075513/morphology-of-brood-pouch-formation-in-the-pot-bellied-seahorse-hippocampus-abdominalis
#18
Mari Kawaguchi, Ryohei Okubo, Akari Harada, Kazuki Miyasaka, Kensuke Takada, Junya Hiroi, Shigeki Yasumasu
BACKGROUND: The reproductive strategies of vertebrates are diverse. Seahorses (Pisces: Syngnathidae) possess the unique characteristic of male pregnancy; i.e., males, not females, incubate embryos in a specialized structure called a 'brood pouch'. The brood pouch is formed along the ventral midline of the tail. The lumen of the brood pouch is surrounded by loose connective tissue, called pseudoplacenta, and dermis. RESULTS: We visualized and evaluated the morphology of brood pouch formation in Hippocampus abdominalis to gain generalizable insights into this process in seahorses...
2017: Zoological Letters
https://www.readbyqxmd.com/read/29053425/association-of-mannose-binding-lectin-rs1800450-and-tumor-necrotic-factor-%C3%AE-rs1800620-polymorphism-with-helicobacter-pylori-in-type-ii-diabetes-mellitus
#19
Elnaz Mortazavi, Behnaz Eslami, Parisa Aghahosseini, Fatemeh Ahron, Armagan Amininejad, Sepideh Mahmoodi, Hadis Satarpour, Nilofar Radmanesh, Hossein Rassi
Type II diabetes mellitus (T2DM) is the prevalent type of diabetes, including 90% of the cases world-wide. Helicobacter pylori plays a pathogenic role in the development of T2DM. The host genetic factors have a significant impact on the clinical outcome and anatomical distribution of H. pylori infection and polymorphisms in several genes such as tumor necrotic factor (TNF)-α and mannose-binding lectin (MBL) and are considered to increase the risk for the development of T2DM. In this study, we investigate the prevalence rate of H...
October 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28988747/structural-insights-into-vlr-fine-specificity-for-blood-group-carbohydrates
#20
Bernard C Collins, Robin J Gunn, Tanya R McKitrick, Richard D Cummings, Max D Cooper, Brantley R Herrin, Ian A Wilson
High-quality reagents to study and detect glycans with high specificity for research and clinical applications are severely lacking. Here, we structurally and functionally characterize several variable lymphocyte receptor (VLR)-based antibodies from lampreys immunized with O erythrocytes that specifically recognize the blood group H-trisaccharide type II antigen. Glycan microarray analysis and biophysical data reveal that these VLRs exhibit greater specificity for H-trisaccharide compared with the plant lectin UEA-1, which is widely used in blood typing...
November 7, 2017: Structure
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