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https://www.readbyqxmd.com/read/29471500/molecular-identification-of-leishmania-spp-in-sand-flies-diptera-psychodidae-phlebotominae-in-the-len%C3%A3-%C3%A3-is-maranhenses-national-park-brazil
#1
Adalberto Alves Pereira-Filho, Raquel Silva Fonteles, Maria da Conceição Abreu Bandeira, Jorge Luiz Pinto Moraes, José Manuel Macário Rebêlo, Maria Norma Melo
Sand flies are very common in the region of Lençóis Maranhenses National Park, an important tourist attraction in Brazil. However, the role of some species and their relative importance locally in Leishmania Ross 1903 transmission is unclear. The objective of this study was to identify Leishmania infection in phlebotomine sand flies collected around the Lençóis Maranhenses National Park, an important conservation area and popular international/national tourist destination with a high incidence of leishmaniasis...
February 20, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29470862/arabidopsis-hipp27-is-a-host-susceptibility-gene-for-the-beet-cyst-nematode-heterodera-schachtii
#2
Zoran S Radakovic, Muhammad Shahzad Anjam, Elizabeth Escobar, Divykriti Chopra, Javier Cabrera, Ana Cláudia Silva, Carolina Escobar, Miroslaw Sobczak, Florian M W Grundler, Shahid Siddique
Sedentary plant-parasitic cyst nematodes are obligate biotrophs that infect the roots of their host plant. Their parasitism is based on modification of root cells to form a hypermetabolic syncytium from which the nematodes draw their nutrients. The aim of this study was to identify nematode susceptibility genes in Arabidopsis thaliana and to characterize their roles in supporting the parasitism of Heterodera schachtii. By selecting genes that were most strongly upregulated in response to cyst nematode infection, we identified HIPP27 (HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 27) as a host susceptibility factor required for beet cyst nematode infection and development...
February 22, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29470856/leishmania-donovani-inhibits-ferroportin-translation-by-modulating-fbxl5-irp2-axis-for-its-growth-within-host-macrophages
#3
Nupur Kanti Das, Sandhya Sandhya, G Vishnu Vivek, Rajiv Kumar, Amit Kumar Singh, Saswat Kumar Bal, Sanju Kumari, Chinmay K Mukhopadhyay
Hepcidin mediated ferroportin (Fpn) degradation in macrophages is a well adopted strategy to limit iron availability towards invading pathogens. Leishmania donovani (LD), a protozoan parasite, resides within macrophage and competes with host for availing iron. Using in vitro and in vivo model of infection we reveal that LD decreases Fpn abundance in host macrophages by hepcidin independent mechanism. Unaffected level of Fpn-FLAG in LD infected J774 macrophage confirms that Fpn down-regulation is not due its degradation...
February 22, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29470711/mast-cell-nerve-interaction-in-the-colon-of-trypanosoma-cruzi-infected-individuals-with-chagasic-megacolon
#4
Patrícia Rocha Martins, Rodolfo Duarte Nascimento, Aline Tomaz Dos Santos, Enio Chaves de Oliveira, Patricia Massara Martinelli, Débora d'Avila Reis
Chagas disease is an infection caused by the parasite Trypanosoma cruzi that affects millions of people worldwide and is endemic in Latin America. Megacolon is the most frequent complication of the digestive chronic form and happens due to lesions of the enteric nervous system. The neuronal lesions seem to initiate in the acute phase and persist during the chronic phase, albeit the mechanisms involved in this process are still debated. Among the cells of the immune system possibly involved in this pathological process is the mast cell (MC) due to its well-known role in the bi-directional communication between the immune and nervous systems...
February 22, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29470556/no-evidence-for-association-between-apol1-kidney-disease-risk-alleles-and-human-african-trypanosomiasis-in-two-ugandan-populations
#5
Magambo Phillip Kimuda, Harry Noyes, Julius Mulindwa, John Enyaru, Vincent Pius Alibu, Issa Sidibe, Dieuodonne Mumba Ngoyi, Christiane Hertz-Fowler, Annette MacLeod, Özlem Tastan Bishop, Enock Matovu
BACKGROUND: Human African trypanosomiasis (HAT) manifests as an acute form caused by Trypanosoma brucei rhodesiense (Tbr) and a chronic form caused by Trypanosoma brucei gambiense (Tbg). Previous studies have suggested a host genetic role in infection outcomes, particularly for APOL1. We have undertaken candidate gene association studies (CGAS) in a Ugandan Tbr and a Tbg HAT endemic area, to determine whether polymorphisms in IL10, IL8, IL4, HLAG, TNFA, TNX4LB, IL6, IFNG, MIF, APOL1, HLAA, IL1B, IL4R, IL12B, IL12R, HP, HPR, and CFH have a role in HAT...
February 22, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29470516/evaluation-of-diagnostic-methods-for-the-detection-of-intestinal-schistosomiasis-in-endemic-areas-with-low-parasite-loads-saline-gradient-helmintex-kato-katz-and-rapid-urine-test
#6
Warllem Junio Oliveira, Fernanda do Carmo Magalhães, Andressa Mariana Saldanha Elias, Vanessa Normandio de Castro, Vivian Favero, Catieli Gobetti Lindholz, Áureo Almeida Oliveira, Fernando Sergio Barbosa, Frederico Gil, Maria Aparecida Gomes, Carlos Graeff-Teixeira, Martin Johannes Enk, Paulo Marcos Zech Coelho, Mariângela Carneiro, Deborah Aparecida Negrão-Corrêa, Stefan Michael Geiger
BACKGROUND: In some tropical countries, such as Brazil, schistosomiasis control programs have led to a significant reduction in the prevalence and parasite burden of endemic populations. In this setting, the Kato-Katz technique, as the standard diagnostic method for the diagnosis of Schistosoma mansoni infections, which involves the analysis of two slides from one fecal sample, loses its sensitivity. As a result, a significant number of infected individuals are not detected. The objective of this study was to perform extensive parasitological testing of up to three fecal samples and include a rapid urine test (POC-CCA) in a moderate prevalence area in Northern Minas Gerais, Brazil, and evaluate the performance of each test separately and in combination...
February 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29469990/differential-metabolic-pathway-analysis-of-the-proteomes-of-leishmania-donovani-and-leptomonas-seymouri
#7
Neeloo Singh, Renu Goel, Ekta Jain
PURPOSE: Although in trypanosomatids, monoxeny (Leptomonas) is ancestral to dixeny (Leishmania), however clinical cases of visceral leishmanisis with Leptomonas co-infection are increasingly being reported from India. Using a proteogenomic approach, we carried out a detailed proteome analysis of these two kinetoplastid parasites viz. Leishmania and its sister Leptomonas, to catalog the key proteins associated with and therefore possibly responsible for phenotype changes in Leptomonas evolution and domestication as co-infection with Leishmania...
February 22, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29469522/-in-vitro-pro-angiogenic-activity-of-echinococcus-granulosus-hydatid-cysts-from-experimentally-infected-mice
#8
Yin Jian-Hai, Shen Yu-Juan, Yu Ai-Ping, Cao Jian-Ping
OBJECTIVE: To preliminarily study the pro-angiogenic activity of Echinococcus granulosus hydatid cysts against human umbilical vein endothelial cells in vitro and the transcriptional level of potential pro-angiogenic factors. METHODS: The hydatid cysts and protoscolex derived from experimentally infected mice were collected and cultured in vitro , then the human umbilical vein endothelial cells were stimulated by the supernatant and cyst fluid respectively, and the angiogenesis was observed and analyzed through a microscope and the angiogenesis mode of the software NIH Image J...
May 23, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469460/-knowledge-attitude-and-practice-kap-of-foodborne-parasitic-diseases-among-middle-school-students-in-xuzhou-city
#9
Zhou Qi, Liu Ya-Peng, Li Li
OBJECTIVE: To investigate the status of knowledge, attitude and practice (KAP) of foodborne parasitic diseases among middle school students in Xuzhou City, so as to provide a reference to the health education. METHODS: A total of four middle schools were selected and their students were investigated with the basic information questionnaire and questions of foodborne parasitic diseases. RESULTS: The awareness rates of parasitic diseases, hazards and transmission were 56...
December 26, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469448/-trends-of-soil-transmitted-nematode-infections-in-jiangxi-province
#10
Zeng Xiao-Jun, Jiang Wei-Sheng, Xie Shu-Ying, Ge Jun, Li Zhao-Jun, Hang Chun-Qin, Liu Yue-Min, Yi Fang-Yu, Chen Hong-Gen
OBJECTIVE: To analyze the status and trends of soil-transmitted nematode infections in Jiangxi Province from 1989 to 2014, so as to provide the evidence for generating the strategy of soil-transmitted nematode prevention and control. METHODS: The data of three epidemiological surveys on human parasitic diseases (in 1989, 2002 and 2014) were classified and analyzed. The stool examination by Kato-Katz's thick smear method was adopted for the investigation of soil-transmitted nematode infections...
December 5, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469443/-studies-on-resistance-of-schistosoma-to-praziquantel-xvii-biological-characteristics-of-praziquantel-resistant-isolates-of-schistosoma-japonicum-in-mice
#11
Qian Ke, Liang You-Sheng, Wang Wei, Qu Guo-Li, Li Hong-Jun, Yang Zhen-Kun, Zhao Zheng-Yang, Xing Yuntian, Dai Jian-Rong
OBJECTIVE: To investigate the biological characteristics of the praziquantel-resistant isolate of Schistosoma japonicum in mice, so as to explore the pathogenicity to definitive hosts and transmission intensity of the praziquantel-resistant isolate of S. japonicum . METHODS: Mice were infected with the cercariae released from two praziquantel-resistant isolates and two praziquantel-susceptible isolates of S. japonicum . The mouse- Oncomelania hupensis snail-mouse cycle was established and maintained in the laboratory...
December 26, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469442/-studies-on-resistance-of-schistosoma-to-praziquantel-x%C3%A2-biological-characteristics-of-praziquantel-resistant-isolates-of-schistomoma-japonicum-in-oncomelania-hupensis-snails
#12
Qian Ke, Liang You-Sheng, Wang Wei, Qu Guo-Li, Li Hong-Jun, Yang Zhen-Kun, Zhao Zheng-Yang, Xing Yuntian, Dai Jian-Rong
OBJECTIVE: To investigate the difference of biological characteristics between the praziquantel-resistant and -susceptible isolates of Schistosoma japonicum in intermediate host Oncomelania hupensis snails. METHODS: Mice were infected with cercariae of praziquantel-resistant and -susceptible isolates of S. japonicum , and the parasite eggs were collected 37 days post-infection to hatch miracidium. Then, the snails were infected with the miracidium of each parasite isolate...
December 26, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469368/-survey-of-human-major-parasitic-diseases-in-shixing-county-from-2002-to-2016
#13
Deng Yue-Jiao, Xie Lun-Geng, Huang Chun-Yan, Zhang Shi-Wu
OBJECTIVE: To analyze the results of 3 investigations (2002-2004, 2008-2009, and 2016) of major parasitic diseases in Shixing County, so as to provide the evidence for formulating further control strategies. METHODS: With the stratified cluster sampling method, 5 administrative villages were investigated. A total of 200 residents aged above 3 years were sampled in each village to investigate the eggs of soil-transmitted nematodes with Kato-Katz technique, and the swab method was applied for detecting the eggs of Enterobius vermicularis in children aged 3-9 years...
July 28, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469367/-analysis-of-human-intestinal-nematode-infections-in-nanjing-city-from-2006-to-2015
#14
He Yi-Sha, Li Yan-Jing, Xie Chao-Yong
OBJECTIVE: To analyze the epidemiological situation of human intestinal nematode infections in Nanjing City from 2006 to 2015, so as to provide the reference for formulating prevention and control measures. METHODS: The surveillance data of human intestinal nematode infections in Nanjing City from 2006 to 2015 were collected and analyzed statistically. RESULTS: From 2006 to 2015, 98 804 person-times of residents were surveyed in Nanjing City, and 465 person-times of residents were detected with intestinal nematode infections...
July 28, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469358/-survey-and-analysis-of-epidemic-status-of-principal-human-parasitosis-in-ecological-region-of-huaiyang-hills-of-henan-province-in-2015
#15
Zhang Ya-Lan, Zhu Yan-Kun, Chen Wei-Qi, Deng Yan, Lin Xi-Meng, Li Peng, Zhang Hong-Wei
OBJECTIVE: To understand the epidemic status of principal human parasitosis in the ecological region of Huaiyang hills of Henan Province. METHODS: According to the scheme of The 3rd National Survey of Principal Human Parasites made by National Institute of Parasitic Diseases, the survey was performed based on the ecological regions. The stratified cluster sampling was made combined with the economic and geographical conditions. The infections of intestinal helminths and protozoans in permanent residents were respectively detected by Kato-Kats technique and iodine solution...
July 24, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469342/-precision-control-is-essential-to-achieve-the-goal-of-schistosomiasis-elimination-in-china-summary-of-the-second-forum-on-schistosomiasis-control-in-china
#16
(no author information available yet)
The Second Forum on Schistosomiasis Control in China was successfully held in Jiangsu Institute of Parasitic Diseases during the period between 21th and 23th, September, 2017. Based on the topic "Implementation of precision control to accelerate the progress towards schistosomiasis elimination", the forum discussed three macro problems pertaining to how to improve the theory and policy of precision control during the progress towards schistosomiasis elimination in China, and six technical problems regarding how to achieve more precise management of the residual sources of infections and more precise monitoring of transmission risk...
October 15, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469323/-molecular-epidemiology-of-cryptosporidium-infection-in-infants-with-diarrhea-in-wuhan-city
#17
Liu Xiao-Jie, Mao Tie-Bo, Wu Peng, Zhou Rui
OBJECTIVE: To understand the prevalence of Cryptosporidium infection in diarrhea infants under 2 years old in Wuhan City, so as to provide the epidemiological evidence for the prevention and treatment of cryptosporidiosis. METHODS: The fecal samples from infants under 2 years old with diarrhea were collected in Hubei General Hospital and Central South Hospital in Wuhan City, Hubei Province from August 2014 to July 2015. The fecal samples were stored in 2.5% potassium dichromate at 4 ℃ after filtered...
March 14, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29468033/recolonizing-gray-wolves-increase-parasite-infection-risk-in-their-prey
#18
Ines Lesniak, Ilja Heckmann, Mathias Franz, Alex D Greenwood, Emanuel Heitlinger, Heribert Hofer, Oliver Krone
The recent recolonization of Central Europe by the European gray wolf ( Canis lupus ) provides an opportunity to study the dynamics of parasite transmission for cases when a definitive host returns after a phase of local extinction. We investigated whether a newly established wolf population increased the prevalence of those parasites in ungulate intermediate hosts representing wolf prey, whether some parasite species are particularly well adapted to wolves, and the potential basis for such adaptations. We recorded Sarcocystis species richness in wolves and Sarcocystis prevalence in ungulates harvested in study sites with and without permanent wolf presence in Germany using microscopy and DNA metabarcoding...
February 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29468014/a-comparative-analysis-of-wolbachia-induced-host-reproductive-phenotypes-reveals-transition-rate-heterogeneity
#19
Heinrich Zu Dohna, Carine Houry, Zakaria Kambris
The endosymbiotic bacterium Wolbachia infects a wide range of arthropods and their relatives. It is an intracellular parasite transmitted through the egg from mother to offspring. Wolbachia can spread and persist through various means of host reproductive manipulation. How these different mechanisms of host manipulation evolved in Wolbachia is unclear. Which host reproductive phenotype is most likely to be ancestral and whether evolutionary transitions between some host phenotypes are more common than others remain unanswered questions...
February 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29467757/genetic-regulation-of-guanylate-binding-proteins-2b-and-5-during-leishmaniasis-in-mice
#20
Yahya Sohrabi, Valeryia Volkova, Tatyana Kobets, Helena Havelková, Imtissal Krayem, Martina Slapničková, Peter Demant, Marie Lipoldová
Interferon-induced GTPases [guanylate-binding proteins (GBPs)] play an important role in inflammasome activation and mediate innate resistance to many intracellular pathogens, but little is known about their role in leishmaniasis. We therefore studied expression of Gbp2b / Gbp1 and Gbp5 mRNA in skin, inguinal lymph nodes, spleen, and liver after Leishmania major infection and in uninfected controls. We used two different groups of related mouse strains: BALB/c, STS, and CcS-5, CcS-16, and CcS-20 that carry different combinations of BALB/c and STS genomes, and strains O20, C57BL/10 (B10) and B10...
2018: Frontiers in Immunology
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