keyword
MENU ▼
Read by QxMD icon Read
search

PerR protein

keyword
https://www.readbyqxmd.com/read/29133393/sequential-induction-of-fur-regulated-genes-in-response-to-iron-limitation-in-bacillus-subtilis
#1
Hualiang Pi, John D Helmann
Bacterial cells modulate transcription in response to changes in iron availability. The ferric uptake regulator (Fur) senses intracellular iron availability and plays a central role in maintaining iron homeostasis in Bacillus subtilis Here we utilized FrvA, a high-affinity Fe(2+) efflux transporter from Listeria monocytogenes, as an inducible genetic tool to deplete intracellular iron. We then characterized the responses of the Fur, FsrA, and PerR regulons as cells transition from iron sufficiency to deficiency...
November 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29076974/on-the-role-of-illness-duration-and-nutrient-restriction-in-cholestatic-alterations-that-occur-during-critical-illness
#2
Marc Jenniskens, Fabian Güiza, Marlies Oorts, Sarah Vander Perre, Sarah Derde, Thomas Dufour, Steven Thiessen, Pieter Annaert, Greet Van den Berghe, Lies Langouche
BACKGROUND AND AIMS: Elevated markers of cholestasis are common in response to critical illness, and associated with adverse outcome. The role of illness duration and of nutrient restriction on underlying molecular pathways of such cholestatic responses have not been thoroughly investigated. METHODS: In a mouse model of surgery- and sepsis-induced critical illness, molecular pathways of cholestasis were investigated up to 7 days. To assess which changes are explained by illness-induced lack of feeding, nutrient-restricted healthy mice were studied and compared with ad libitum fed healthy mice...
October 25, 2017: Shock
https://www.readbyqxmd.com/read/28938859/redox-sensing-by-fe-2-in-bacterial-fur-family-metalloregulators
#3
Azul Pinochet-Barros, John D Helmann
SIGNIFICANCE: Iron is required for growth and is often redox active under cytosolic conditions. As a result of its facile redox chemistry, iron homeostasis is intricately involved with oxidative stress. Bacterial adaptation to iron limitation and oxidative stress often involves ferric uptake regulator (Fur) proteins: a diverse set of divalent cation-dependent, DNA-binding proteins that vary widely in both metal selectivity and sensitivity to metal-catalyzed oxidation. Recent Advances: Bacteria contain two Fur family metalloregulators that use ferrous iron (Fe(2+)) as their cofactor, Fur and PerR...
October 31, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28434086/the-inability-of-bacillus-licheniformis-perr-mutant-to-grow-is-mainly-due-to-the-lack-of-perr-mediated-fur-repression
#4
Jung-Hoon Kim, Yoon-Mo Yang, Chang-Jun Ji, Su-Hyun Ryu, Young-Bin Won, Shin-Yeong Ju, Yumi Kwon, Yeh-Eun Lee, Hwan Youn, Jin-Won Lee
PerR, a member of Fur family protein, is a metal-dependent H2O2 sensing transcription factor that regulates genes involved in peroxide stress response. Industrially important bacterium Bacillus licheniformis contains three PerR-like proteins (PerRBL, PerR2, and PerR3) compared to its close relative Bacillus subtilis. Interestingly, unlike other bacteria including B. subtilis, no authentic perR BL null mutant could be established for B. licheniformis. Thus, we constructed a conditional perR BL mutant using a xylose-inducible promoter, and investigated the genes under the control of PerRBL...
June 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28303483/proliferation-and-differentiation-of-adipose-tissue-in-prolonged-lean-and-obese-critically-ill-patients
#5
Chloë Goossens, Sarah Vander Perre, Greet Van den Berghe, Lies Langouche
BACKGROUND: In prolonged non-obese critically ill patients, preservation of adipose tissue is prioritized over that of the skeletal muscle and coincides with increased adipogenesis. However, we recently demonstrated that in obese critically ill mice, this priority was switched. In the obese, the use of abundantly available adipose tissue-derived energy substrates was preferred and counteracted muscle wasting. These observations suggest that different processes are ongoing in adipose tissue of lean vs...
December 2017: Intensive Care Medicine Experimental
https://www.readbyqxmd.com/read/28104400/the-difference-in-in%C3%A2-vivo-sensitivity-between-bacillus-licheniformis-perr-and-bacillus-subtilis-perr-is-due-to-the-different-cellular-environments
#6
Jung-Hoon Kim, Young-Bin Won, Chang-Jun Ji, Yoon-Mo Yang, Su-Hyun Ryu, Shin-Yeong Ju, Yumi Kwon, Yeh-Eun Lee, Jin-Won Lee
PerR, a member of Fur family of metal-dependent regulators, is a major peroxide sensor in many Gram positive bacteria, and controls the expression of genes involved in peroxide resistance. Bacillus licheniformis, a close relative to the well-studied model organism Bacillus subtilis, contains three PerR-like proteins (PerRBL, PerR2 and PerR3) in addition to Fur and Zur. In the present study, we characterized the role of PerRBL in B. licheniformis. In vitro and in vivo studies indicate that PerRBL, like PerRBS, uses either Fe(2+) or Mn(2+) as a corepressor and only the Fe(2+)-bound form of PerRBL senses low levels of H2O2 by iron-mediated histidine oxidation...
February 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28099473/the-staphylococcus-aureus-srrab-regulatory-system-modulates-hydrogen-peroxide-resistance-factors-which-imparts-protection-to-aconitase-during-aerobic-growth
#7
Ameya A Mashruwala, Jeffrey M Boyd
The SrrAB two-component regulatory system (TCRS) positively influences the transcription of genes involved in aerobic respiration in response to changes in respiratory flux. Hydrogen peroxide (H2O2) can arise as a byproduct of spontaneous interactions between dioxygen and components of respiratory pathways. H2O2 damages cellular factors including protein associated iron-sulfur cluster prosthetic groups. We found that a Staphylococcus aureus strain lacking the SrrAB two-component regulatory system (TCRS) is sensitive to H2O2 intoxication...
2017: PloS One
https://www.readbyqxmd.com/read/27935957/intracellular-zn-ii-intoxication-leads-to-dysregulation-of-the-perr-regulon-resulting-in-heme-toxicity-in-bacillus-subtilis
#8
Pete Chandrangsu, John D Helmann
Transition metal ions (Zn(II), Cu(II)/(I), Fe(III)/(II), Mn(II)) are essential for life and participate in a wide range of biological functions. Cellular Zn(II) levels must be high enough to ensure that it can perform its essential roles. Yet, since Zn(II) binds to ligands with high avidity, excess Zn(II) can lead to protein mismetallation. The major targets of mismetallation, and the underlying causes of Zn(II) intoxication, are not well understood. Here, we use a forward genetic selection to identify targets of Zn(II) toxicity...
December 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27814344/subclinical-mastitis-occurs-frequently-in-association-with-dramatic-changes-in-inflammatory-anti-inflammatory-breast-milk-components
#9
Edouard Tuaillon, Johanes Viljoen, Pierre Dujols, Gilles Cambonie, Pierre-Alain Rubbo, Nicolas Nagot, Ruth M Bland, Stéphanie Badiou, Marie-Louise Newell, Philippe Van de Perre
BACKGROUND: Subclinical mastitis (SCM) is a frequent, but poorly characterized entity that may influence immune development of breastfed infants. Mechanisms driving the emergence of SCM and changes in immunological content of human milk during SCM remain to be explored. In this study, the breast milk environment was to describe during SCM. METHODS: One hundred and ten samples of mature breast milk were collected from 44 healthy, HIV-negative mothers, included in a large infant feeding intervention cohort (ANRS 1271/Vertical Transmission Study)...
April 2017: Pediatric Research
https://www.readbyqxmd.com/read/27703070/cap0037-a-novel-global-regulator-of-clostridium-acetobutylicum-metabolism
#10
Ngoc-Phuong-Thao Nguyen, Sonja Linder, Stefanie K Flitsch, Bettina Schiel-Bengelsdorf, Peter Dürre, Philippe Soucaille
An operon comprising two genes, CA_P0037 and CA_P0036, that encode proteins of unknown function that were previously shown to be highly expressed in acidogenic cells and repressed in solventogenic and alcohologenic cells is located on the pSOL1 megaplasmid of Clostridium acetobutylicum upstream of adhE2 A CA_P0037::int (189/190s) mutant in which an intron was inserted at position 189/190 in the sense strand of CA_P0037 was successfully generated by the Targetron technique. The resultant mutant showed significantly different metabolic flux patterns in acidogenic (producing mainly lactate, butyrate, and butanol) and alcohologenic (producing mainly butyrate, acetate, and lactate) chemostat cultures but not in solventogenic or batch cultures...
October 4, 2016: MBio
https://www.readbyqxmd.com/read/27676423/circulating-3-t1am-and-3-5-t2-in-critically-ill-patients-a-cross-sectional-observational-study
#11
Lies Langouche, Ina Lehmphul, Sarah Vander Perre, Josef Köhrle, Greet Van den Berghe
BACKGROUND: Critical illness is hallmarked by low circulating thyroxine (T4) and triiodothyronine (T3) concentrations, in the presence of elevated reverse T3 (rT3) and low-normal thyrotropin (TSH), referred to as nonthyroidal illness (NTI). Thyroid hormone (TH) metabolism is substantially increased during NTI, in part explained by enhanced deiodinase 3 (D3) activity. T4- and T3-sulfate concentrations are elevated, due to suppressed D1 activity in the presence of unaltered sulfotransferase activity, and 3,3'-diiodothyronine (3,3'-T2) concentrations are normal...
December 2016: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/27225459/pprm-is-necessary-for-up-regulation-of-kate1-encoding-the-major-catalase-of-deinococcus-radiodurans-under-unstressed-culture-conditions
#12
Sun-Wook Jeong, Ho Seong Seo, Min-Kyu Kim, Jong-Il Choi, Heon-Man Lim, Sangyong Lim
Deinococcus radiodurans is a poly-extremophilic organism, capable of tolerating a wide variety of different stresses, such as gamma/ultraviolet radiation, desiccation, and oxidative stress. PprM, a cold shock protein homolog, is involved in the radiation resistance of D. radiodurans, but its role in the oxidative stress response has not been investigated. In this study, we investigated the effect of pprM mutation on catalase gene expression. pprM disruption decreased the mRNA and protein levels of KatE1, which is the major catalase in D...
June 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27176811/bacillus-licheniformis-contains-two-more-perr-like-proteins-in-addition-to-perr-fur-and-zur-orthologues
#13
Jung-Hoon Kim, Chang-Jun Ji, Shin-Yeong Ju, Yoon-Mo Yang, Su-Hyun Ryu, Yumi Kwon, Young-Bin Won, Yeh-Eun Lee, Hwan Youn, Jin-Won Lee
The ferric uptake regulator (Fur) family proteins include sensors of Fe (Fur), Zn (Zur), and peroxide (PerR). Among Fur family proteins, Fur and Zur are ubiquitous in most prokaryotic organisms, whereas PerR exists mainly in Gram positive bacteria as a functional homologue of OxyR. Gram positive bacteria such as Bacillus subtilis, Listeria monocytogenes and Staphylococcus aureus encode three Fur family proteins: Fur, Zur, and PerR. In this study, we identified five Fur family proteins from B. licheniformis: two novel PerR-like proteins (BL00690 and BL00950) in addition to Fur (BL05249), Zur (BL03703), and PerR (BL00075) homologues...
2016: PloS One
https://www.readbyqxmd.com/read/26946370/the-listeria-monocytogenes-fur-regulated-virulence-protein-frva-is-an-fe-ii-efflux-p1b4-type-atpase
#14
Hualiang Pi, Sarju J Patel, José M Argüello, John D Helmann
Listeria monocytogenes FrvA (Lmo0641) is critical for virulence in the mouse model and is an ortholog of the Bacillus subtilis Fur- and PerR-regulated Fe(II) efflux P1B4 -type ATPase PfeT. Previously, FrvA was suggested to protect against heme toxicity. Here, we demonstrate that an frvA mutant is sensitive to iron intoxication, but not to other metals. Expression of frvA is induced by high iron and this induction requires Fur. FrvA functions in vitro as a divalent cation specific ATPase most strongly activated by ferrous iron...
June 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/26873375/virulence-factors-of-streptococcus-pyogenes-strains-from-women-in-peri-labor-with-invasive-infections
#15
E Golińska, M van der Linden, G Więcek, D Mikołajczyk, A Machul, A Samet, A Piórkowska, M Dorycka, P B Heczko, M Strus
Invasive group A streptococcal (GAS) infections constitute an important epidemiological problem. Many cases occur in women during the postnatal period. The objective of this study was to evaluate the presence of the genes responsible for production of iron-chelating protein (perR) and superantigens (speA, speB, speC, speF, speG, speH, speI, speJ, speK, speL, speM, smeZ, and ssa) in S. pyogenes strains isolated from invasive infections in women after delivery. Furthermore, this study sought to verify whether S...
May 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/26677871/oxidization-without-substrate-unfolding-triggers-proteolysis-of-the-peroxide-sensor-perr
#16
Bo-Eun Ahn, Tania A Baker
Peroxide operon regulator (PerR) is a broadly conserved hydrogen peroxide sensor in bacteria, and oxidation of PerR at its regulatory metal-binding site is considered irreversible. Here, we tested whether this oxidation specifically targets PerR for proteolysis. We find that oxidizing conditions stimulate PerR degradation in vivo, and LonA is the principal AAA+ (ATPases associated with diverse cellular activities) protease that degrades PerR. Degradation of PerR by LonA is recapitulated in vitro, and biochemical dissection of this degradation reveals that the presence of regulatory metal and PerR-binding DNA dramatically extends the half-life of the protein...
January 5, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/26261021/pfet-a-p1b4-type-atpase-effluxes-ferrous-iron-and-protects-bacillus-subtilis-against-iron-intoxication
#17
Guohua Guan, Azul Pinochet-Barros, Ahmed Gaballa, Sarju J Patel, José M Argüello, John D Helmann
Iron is an essential element for nearly all cells and limited iron availability often restricts growth. However, excess iron can also be deleterious, particularly when cells expressing high affinity iron uptake systems transition to iron rich environments. Bacillus subtilis expresses numerous iron importers, but iron efflux has not been reported. Here, we describe the B. subtilis PfeT protein (formerly YkvW/ZosA) as a P1B4 -type ATPase in the PerR regulon that serves as an Fe(II) efflux pump and protects cells against iron intoxication...
November 2015: Molecular Microbiology
https://www.readbyqxmd.com/read/26141822/refined-analysis-of-the-campylobacter-jejuni-iron-dependent-independent-fur-and-perr-transcriptomes
#18
James Butcher, Rebecca A Handley, Arnoud H M van Vliet, Alain Stintzi
BACKGROUND: The genome of Campylobacter jejuni contains two iron activated Fur-family transcriptional regulators, CjFur and CjPerR, which are primarily responsible for regulating iron homeostasis and oxidative stress respectively. Both transcriptional regulators have been previously implicated in regulating diverse functions beyond their primary roles in C. jejuni. To further characterize their regulatory networks, RNA-seq was used to define the transcriptional profiles of C. jejuni NCTC11168 wild type, Δfur, ΔperR and ΔfurΔperR isogenic deletion mutants under both iron-replete and iron-limited conditions...
2015: BMC Genomics
https://www.readbyqxmd.com/read/26134568/staphylococcus-aureus-perr-is-a-hypersensitive-hydrogen-peroxide-sensor-using-iron-mediated-histidine-oxidation
#19
Chang-Jun Ji, Jung-Hoon Kim, Young-Bin Won, Yeh-Eun Lee, Tae-Woo Choi, Shin-Yeong Ju, Hwan Youn, John D Helmann, Jin-Won Lee
In many Gram-positive bacteria PerR is a major peroxide sensor whose repressor activity is dependent on a bound metal cofactor. The prototype for PerR sensors, the Bacillus subtilis PerRBS protein, represses target genes when bound to either Mn(2+) or Fe(2+) as corepressor, but only the Fe(2+)-bound form responds to H2O2. The orthologous protein in the human pathogen Staphylococcus aureus, PerRSA, plays important roles in H2O2 resistance and virulence. However, PerRSA is reported to only respond to Mn(2+) as corepressor, which suggests that it might rely on a distinct, iron-independent mechanism for H2O2 sensing...
August 14, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26070785/transcription-factors-that-defend-bacteria-against-reactive-oxygen-species
#20
REVIEW
James A Imlay
Bacteria live in a toxic world in which their competitors excrete hydrogen peroxide or superoxide-generating redox-cycling compounds. They protect themselves by activating regulons controlled by the OxyR, PerR, and SoxR transcription factors. OxyR and PerR sense peroxide when it oxidizes key thiolate or iron moieties, respectively; they then induce overlapping sets of proteins that defend their vulnerable metalloenzymes. An additional role for OxyR in detecting electrophilic compounds is possible. In some nonenteric bacteria, SoxR appears to control the synthesis and export of redox-cycling compounds, whereas in the enteric bacteria it defends the cell against the same agents...
2015: Annual Review of Microbiology
keyword
keyword
15092
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"